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Analysis of the Functions and Values of Marine Ecosystems 海洋生态系统的功能与价值分析
Pub Date : 2023-01-01 DOI: 10.5376/ijms.2023.13.0003
Jin Wu, Jia Xuan
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引用次数: 0
Classification and Main Characteristics of Marine Ecosystems 海洋生态系统的分类与主要特征
Pub Date : 2023-01-01 DOI: 10.5376/ijms.2023.13.0002
Jin Wu, L. Jin
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引用次数: 0
Basic Concepts and Practices of Marine Ranching: A Review from Theory to Practice 海洋牧场的基本概念与实践:从理论到实践的回顾
Pub Date : 2023-01-01 DOI: 10.5376/ijms.2023.13.0001
R. Mai
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引用次数: 0
Muscular Coloration Diversity and Microsatellite Genetic Differentiation for the Clam Paphia amabilis (Philippi, 1847) 蛤的肌肉颜色多样性和微卫星遗传分化(Philippi, 1847)
Pub Date : 2021-02-26 DOI: 10.5376/IJMS.2021.11.0001
S. Wang, Xing Wu, F. Yu, Ting-Cong Liao, Qiongzhen Li, B. Luo, Yan Wang
Paphia amabilis  is an important fishery shellfish widely distributing along Southeast Asia coast. The muscles of its foot and siphons are commonly colored bright red-orange due to the presence of C37 skeletal carotenoids. However, 10.5%-18.6% of yellow-muscled individuals (YMs) were found among the four  P .  amabilis  wild samples from Vietnam (Co To island, CT) and China (Beihai, BH/BHA, Guangxi Province; and Zhanjiang, ZJ, Guangdong Province), the north of Beibu Gulf, South China Sea. Twenty genomic microsatellite markers were developed and 12 were employed to analyze the population genetic differentiation of three geographical populations (CT, BH, and ZJ, n=32/population), as well as YM and RM (red-muscled) subpopulations. Medium levels of genetic diversity were detected in all three populations with the number of alleles ranging from 4-21 (8.4±4.5) for CT, 4-16 (7.6±3.3) for BH and 4-18 (7.5±3.7) for ZJ. The average observed/expected heterozygosities were 0.66/0.66 for CT, 0.62/0.63 for BH, and 0.55/0.62 for ZJ, respectively. Total 4 out of 36 tests of microsatellites deviated from Hardy–Weinberg equilibrium (HWE,  P <0.05 after Bonferroni correction), all in ZJ population due to the presence of null alleles suggested by MICRO-CHECK. Exact test indicated highly significant differentiations ( F ST ) between Vietnamese and two Chinese populations (0.099/0.123 between CT and BH/ZJ). No allele of any microsatellite marker was found significantly associated with muscularcoloration of  P. amabilis , consequently no convergent microsatellite genetic differentiation presents between YM and RM subpopulations, supporting that epigenetic factor might play an important role in carotenoids accumulation of  P. amabilis .
巴菲亚是一种重要的渔业贝类,广泛分布在东南亚沿海地区。由于C37类骨骼胡萝卜素的存在,它的足部肌肉和虹吸管通常呈明亮的红橙色。然而,黄肌个体(YMs)占10.5%-18.6%。越南(关岛,CT)和中国(北海,BH/BHA,广西)的野山梨标本;南海北部湾北部的湛江(广东省湛江)。开发了20个基因组微卫星标记,利用12个基因组微卫星标记分析了3个地理群体(CT、BH和ZJ, n=32/个)以及YM和RM(红肌亚群)的群体遗传分化。3个群体的等位基因数量分别为4 ~ 21(8.4±4.5)个、4 ~ 16(7.6±3.3)个和4 ~ 18(7.5±3.7)个,均具有中等水平的遗传多样性。CT、BH和ZJ的平均观察杂合度分别为0.66/0.66、0.62/0.63和0.55/0.62。36个微卫星检测中有4个偏离Hardy-Weinberg平衡(HWE,经Bonferroni校正P <0.05),均发生在ZJ群体中,原因是MICRO-CHECK提示存在零等位基因。未发现任何微卫星标记的等位基因与amabilis肌色显著相关,因此在YM和RM亚群之间不存在趋同的微卫星遗传分化,支持表观遗传因素可能在amabilis类胡萝卜素积累中起重要作用。
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引用次数: 0
Potential Bioactive Compound Seaweed Halimeda micronesica and Halimeda macroloba as Antioxidant and Phytochemical Screening of Active Seaweed Extracts from Maspari Island 具有潜在生物活性的复方微褐藻和大褐藻抗氧化作用及马斯帕里岛活性海藻提取物的植物化学筛选
Pub Date : 2020-10-16 DOI: 10.5376/IJMS.2020.10.0006
M. Hendri, Oktaviana Lubis Delini, Rozirwan Rozirwan
The species H. micronesica and H. macroloba are a group of green seaweed (Chlorophyta) which are found in the waters of  Pulau Maspari. The purposes of this study was to analyze the activity of antioxidant and phytochemicals compounds of the active extract of seaweed H. micronesica and H. macroloba originate from the Maspari Island. The procedures in this study include; collecting and handled samples, stratified maceration both samples using a solvent ethyl acetate (Hmi-EA and Hma-EA) and methanol (Hmi-M and Hma-M), extraction, antioxidants test with DPPH, phytochemical test and analysis IC 50 . The results showed that of the four extracts tested contained an extract that had the ability to inhibit free radicals which extract Hmi-EA. Active extract Hmi-EA had a value of IC 50 52.81 ppm. Active extract Hmi-EA contained alkaloids, steroids and flavonoids. Hmi-EA extract will be highly potential as an antioxidant.
H. micronesica和H. macroloba是一组绿海藻(绿藻),发现于马斯帕里岛的水域。本研究分析了产自马斯帕里岛(Maspari Island)的紫菜H. micronesica和H. macroloba活性提取物的抗氧化活性和植物化学成分的活性。本研究的程序包括;收集和处理样品,使用溶剂乙酸乙酯(Hmi-EA和Hma-EA)和甲醇(Hmi-M和Hma-M)对样品进行分层浸渍,提取,DPPH抗氧化剂测试,植物化学测试和分析IC 50。结果表明,四种提取物中含有一种具有抑制自由基能力的提取物,该提取物可提取Hmi-EA。活性提取物Hmi-EA的ic50为52.81 ppm。活性提取物Hmi-EA含有生物碱、类固醇和黄酮类化合物。Hmi-EA提取物具有很强的抗氧化潜力。
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引用次数: 0
Whale fall: the Creation of A Unique Marine Ecosystem 鲸鱼坠落:一个独特的海洋生态系统的创造
Pub Date : 2020-06-04 DOI: 10.5376/ijms.2020.10.0004
Bess X.Y. Chen, May H.M. Wang
When a whale dies in the ocean, its body slowly sinks to the bottom of the sea. In this process, animals and decomposers gather in the ocean to form an ecosystem. Biologists call this unique process "whale fall." Whale fall, together with ocean cold spring and hydrothermal fluid, is called the "oasis" of the ocean.  Whale carcasses provide a rich source of food for all types of consumers in the vast and barren deep-sea food chain, including deep-sea fish, crustaceans, polychaetes and various small organisms.  This review introduces the necessary conditions, causes and ecological characteristics of whale fall from various perspectives, and analyzes the impact of whale fall on the ocean; A large number of facts show that whale fall not only provides a huge driving force for the marine carbon flux, but also promotes the production of new species and enriches the diversity of marine organisms. Whale falling is a wonderful creation of nature. It is of great significance to understand this phenomenon for us to understand this unique marine ecosystem.
当鲸鱼在海洋中死亡时,它的身体会慢慢沉入海底。在这个过程中,动物和分解者聚集在海洋中形成一个生态系统。生物学家称这种独特的过程为“鲸鱼坠落”。鲸鱼瀑布与海洋冷泉和热液一起被称为海洋的“绿洲”。鲸鱼尸体为广阔而贫瘠的深海食物链中的各类消费者提供了丰富的食物来源,包括深海鱼类、甲壳类动物、多毛类动物和各种小生物。本文从多个角度介绍了鲸落的必要条件、原因和生态特征,并分析了鲸落对海洋的影响;大量事实表明,鲸落不仅为海洋碳通量提供了巨大的动力,而且促进了新物种的产生,丰富了海洋生物的多样性。鲸鱼坠落是大自然奇妙的创造。了解这一现象对我们了解这一独特的海洋生态系统具有重要意义。
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引用次数: 0
Total Hydrocarbon and Heavy Metal Concentrations in Body Parts of Fiddler Crab (Uca tangeri) (Ocipodidae) in the Niger Delta, Nigeria 尼日利亚尼日尔三角洲招潮蟹(Uca tangeri) (Ocipodidae)身体部位的总碳氢化合物和重金属浓度
Pub Date : 2020-04-30 DOI: 10.5376/ijms.2020.10.0001
Aroloye O. Numbere
This study is based on the hypothesis that the burrowing habit of fiddler crabs in polluted environment will predispose them to heavy metal contamination. The study was carried out in Eagle Island, a mangrove community. Thirty fiddler crabs ( Uca tangeri ) were captured by hand and butchered into parts and oven dried at 70°C for 48 hours. They were sent to laboratory to determine total hydrocarbon content (THC), Cadmium (Cd), Zinc (Zn) and Lead (Pb) concentrations. The laboratory procedure involved measurement by spectrophotometric method using the HACH DR 890 colorimeter (wavelength 420 nm) for heavy metals and microwave accelerated reaction system (MARS Xpress, North Carolina) for THC. The result indicate that there was no significant difference in THC and heavy metal concentration between crab parts (F 6, 49 = 0.32, P=0.925). However, Zinc had the highest concentration in the crab parts followed by Lead and Cadmium. Zinc was the highest in body tissue (83.57±17.04) mg/L followed by intestine (70.59±1.54) mg/L and ventral shell (67.44±1.1) mg/L. Lead was highest in ventral shell (44.5±34.5) mg/L while Cadmium was highest in carapace (10.02±3.99) mg/L. The order of concentration in the body parts of U. tangeri is Zn>Pb>Cd>THC. THC and Lead were higher in males than in females while Zinc and Cadmium were higher in females. External parts have higher THC, Cd and Pb while internal parts have higher Zn. This result indicates that heavy metals bioaccumulated in fiddler crabs, which can get biomagnified in humans if consumed.
本研究基于招潮蟹在受污染环境中的穴居习性会使其易受重金属污染的假设。这项研究是在红树群落鹰岛进行的。手捕招潮蟹30只,切成部分,70℃烘箱干燥48小时。他们被送到实验室,以测定总碳氢化合物含量(THC),镉(Cd),锌(Zn)和铅(Pb)浓度。实验程序包括使用HACH DR 890比色计(波长420 nm)分光光度法测量重金属和微波加速反应系统(MARS express, North Carolina)测量四氢大麻酚。结果表明,不同部位的四氢大麻酚和重金属含量差异不显著(f6, 49 = 0.32, P=0.925)。蟹体中锌的含量最高,其次是铅和镉。锌含量最高的是体组织(83.57±17.04)mg/L,其次是肠(70.59±1.54)mg/L,腹壳(67.44±1.1)mg/L。腹壳中铅含量最高(44.5±34.5)mg/L,甲壳中镉含量最高(10.02±3.99)mg/L。丹参体内各部位的浓度顺序为Zn b> Pb b>Cd b> THC。四氢大麻酚和铅在男性中高于女性,锌和镉在女性中高于女性。外置部分THC、Cd、Pb含量较高,内置部分Zn含量较高。这一结果表明,重金属在招潮蟹体内的生物积累,如果食用,会在人体中被生物放大。
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引用次数: 1
Cloning and Expression Analysis of Cdc25 Gene of Sipunculus nudus in Oocytes 裸星虫卵母细胞Cdc25基因的克隆及表达分析
Pub Date : 2020-04-22 DOI: 10.5376/ijms.2020.10.0002
Yong-ping Su, Jianmin Ye, Qi Liu, Yetao Zeng, Chengshun Zhang, Qingheng Wang
Cell division cycle 25 (Cdc25) is an important dual specificity phosphatase, which plays an important role in regulating the process of oocyte meiosis and embryo development. In this study, the full-length cDNA of Sn-Cdc25 was cloned from S. nudus using RACE technology. The results show that Sn-Cdc25 is 4 130 bp in length, including 3′ UTR 1 849 bp and 5′ UTR 427 bp. The Open reading frame (ORF) is 1 854 bp and encodes 617 amino acids. Sequence analysis shows that the molecular weight of Sn-Cdc25 protein is 69.58 kD, with two typical Cdc25 protein domains: M-phase inducer phosphatase domain and Rhodanese-like domain, and the active site sequence HCX 5 R that can catalyze the dephosphorylation process. Multi-sequence alignment finds that the C-terminal homology is higher than the N-terminal. The tertiary structure prediction shows that the spatial conformation of Cdc25 homologous protein and its active site are highly conservative. A total of 5 Motifs are found in Motif analysis, of which Motif 1 and Motif 2 are Paxillin LD motif and MYND domain binding motif, respectively. Phylogenetic tree analysis shows that Cdc25 is clustered into two branches: invertebrates and vertebrates. RT-PCR results show that the expression of Sn-Cdc25 , with two peaks, is significantly different in different developmental stages of oocytes. The increase in the expression of Sn-Cdc25 from primary vitellogenic stage to the late of active vitellogenic stage (O1-O3) may be related to the process of Sn-Cdc25 promoting DNA replication. When the oocytes entering metanephridium from coelomic fluid, the rapid rise of Sn-Cdc25 expression may be beneficial to the activation of maturation promoting factor (MPF). The above results have accumulated basic data for further understanding of the developmental mechanism of Sipuncula oocytes and for optimization of artificial breeding techniques.
细胞分裂周期25 (Cell division cycle 25, Cdc25)是一种重要的双特异性磷酸酶,在调节卵母细胞减数分裂和胚胎发育过程中起重要作用。本研究利用RACE技术从裸鼠中克隆出Sn-Cdc25全长cDNA。结果表明,Sn-Cdc25全长4 130 bp,其中3′UTR为1 849 bp, 5′UTR为427 bp。开放阅读框(ORF)全长1 854 bp,编码617个氨基酸。序列分析表明,Sn-Cdc25蛋白分子量为69.58 kD,具有两个典型的Cdc25蛋白结构域:m期诱导磷酸酶结构域和rhodanese样结构域,活性位点序列hcx5r可催化去磷酸化过程。多序列比对发现,c端同源性高于n端。三级结构预测表明,Cdc25同源蛋白的空间构象及其活性位点高度保守。Motif分析共发现5个Motif,其中Motif 1和Motif 2分别为Paxillin LD Motif和MYND结构域结合Motif。系统发育树分析表明,Cdc25可分为两个分支:无脊椎动物和脊椎动物。RT-PCR结果显示,Sn-Cdc25在卵母细胞不同发育阶段的表达存在显著差异,呈双峰表达。Sn-Cdc25从初发卵黄期到卵黄活跃期晚期(0 ~ 3)表达的增加可能与Sn-Cdc25促进DNA复制的过程有关。当卵母细胞从体腔液进入后肾时,Sn-Cdc25表达的迅速上升可能有利于成熟促进因子(MPF)的激活。以上结果为进一步了解星形虫卵母细胞发育机制和优化人工育种技术积累了基础数据。
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引用次数: 0
Cultivation of Seaweed E.spinosum in Open Waters Using Bags Methods Around Pahawang Island Waters, South Lampung 南楠榜岛巴哈旺岛海域开放水域袋法养殖棘藻
Pub Date : 2020-01-01 DOI: 10.5376/ijms.2020.10.0005
M. Hendri
Seaweed cultivation in Pahawang Island waters have a serious problem like predators from Baronang fish and sea turtle, strong current and big waves also give big impact for seaweed cultivation at there. Bag nets is one of method who can be a solution for that problems. The aim of this research are want to know about daily growth rate (DGR) and absolute growth rate (AGR) from seaweed E. Spinosum. This research has been done in August – September 2019. The net bags was designed to be strong enough for protect seaweed from predation and strong current at there. The net bags prepared are 56 nets, with initial weight of seaweed 50 grams. The result of this research show the daily growth rate (DGR) was 5.6 % each day and for absolute growth rate (AGR) was 304.9 grams. The growth rate is good enough because growth more than 2% each day.
巴哈旺岛海域的海藻养殖存在着男爵鱼和海龟等天敌的严重问题,强流和大浪也给巴哈旺岛海域的海藻养殖带来了很大的影响。袋网是解决这些问题的一种方法。本研究旨在了解棘藻的日生长率(DGR)和绝对生长率(AGR)。这项研究是在2019年8月至9月进行的。这些网袋设计得足够坚固,可以保护海藻不被捕食,也可以保护那里的强流。准备的网袋为56网,初始海藻重量为50克。结果表明:日生长率(DGR)为5.6%,日绝对生长率(AGR)为304.9 g。增长率足够好,因为每天的增长率超过2%。
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引用次数: 0
Development of Microsatellite Markers for the Hermatypic Coral Porites lutea 雌雄同体珊瑚孔隙微卫星标记的建立
Pub Date : 2020-01-01 DOI: 10.5376/ijms.2020.10.0003
Fuyu Li, Dandan Chen, Yuanchao Li, Shiyao Li, Jingqiu Hou, Daoru Wang, Yan Wang
By mining the EST and genomic microsatellite sequence resources of Porites species in GenBank, and using interspecific amplification and resequencing, we developed 20 microsatellite markers for the stony coral Porites lutea, meanwhile, validated and excluded 43 microsatellite markers of its symbiotic zooxanthella. These markers of P. lutea were genotyped in one wild population (n=16) from the southwest of Hainan Island fringing reefs. Nineteen of the 20 markers showed polymorphism. The number of alleles was 2~7 (mean 3.5±1.8), and the observed heterozygosity (Ho) and expected heterozygosity (He) were 0.06~0.94 (mean 0.34±0.29) and 0.18~0.83 (mean 0.57±0.20), respectively. Four markers remained deviated from the Hardy-Weinberg Equilibrium (HWE) after multiple comparison correction for Bonferroin (PHWE<0.05). This is the first set of microsatellite markers of P. lutea. They should be useful for the research on the genetic diversity and connectivity of this species and the conservation of endangered coral reef ecosystems and reef-building coral communities in the Indo-Pacific region.
通过挖掘GenBank中Porites物种的EST和基因组微卫星序列资源,利用种间扩增和重测序,开发了20个石珊瑚Porites lutea的微卫星标记,同时验证并排除了43个其共生虫黄藻的微卫星标记。这些标记在海南岛西南部一个野生种群(n=16)中进行了基因分型。20个标记中有19个存在多态性。等位基因数为2~7个(平均3.5±1.8),观察杂合度(Ho)和期望杂合度(He)分别为0.06~0.94(平均0.34±0.29)和0.18~0.83(平均0.57±0.20)。对Bonferroin进行多次比较校正后,仍有4个标记偏离Hardy-Weinberg平衡(HWE) (PHWE<0.05)。这是黄叶参的第一组微卫星标记。对研究该物种的遗传多样性和连通性,保护印度洋-太平洋地区濒危珊瑚礁生态系统和造礁珊瑚群落具有重要意义。
{"title":"Development of Microsatellite Markers for the Hermatypic Coral Porites lutea","authors":"Fuyu Li, Dandan Chen, Yuanchao Li, Shiyao Li, Jingqiu Hou, Daoru Wang, Yan Wang","doi":"10.5376/ijms.2020.10.0003","DOIUrl":"https://doi.org/10.5376/ijms.2020.10.0003","url":null,"abstract":"By mining the EST and genomic microsatellite sequence resources of Porites species in GenBank, and using interspecific amplification and resequencing, we developed 20 microsatellite markers for the stony coral Porites lutea, meanwhile, validated and excluded 43 microsatellite markers of its symbiotic zooxanthella. These markers of P. lutea were genotyped in one wild population (n=16) from the southwest of Hainan Island fringing reefs. Nineteen of the 20 markers showed polymorphism. The number of alleles was 2~7 (mean 3.5±1.8), and the observed heterozygosity (Ho) and expected heterozygosity (He) were 0.06~0.94 (mean 0.34±0.29) and 0.18~0.83 (mean 0.57±0.20), respectively. Four markers remained deviated from the Hardy-Weinberg Equilibrium (HWE) after multiple comparison correction for Bonferroin (PHWE<0.05). This is the first set of microsatellite markers of P. lutea. They should be useful for the research on the genetic diversity and connectivity of this species and the conservation of endangered coral reef ecosystems and reef-building coral communities in the Indo-Pacific region.","PeriodicalId":22529,"journal":{"name":"The international journal of marine science","volume":"1672 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86516137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
期刊
The international journal of marine science
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