Pub Date : 2023-01-01DOI: 10.5376/ijms.2023.13.0003
Jin Wu, Jia Xuan
{"title":"Analysis of the Functions and Values of Marine Ecosystems","authors":"Jin Wu, Jia Xuan","doi":"10.5376/ijms.2023.13.0003","DOIUrl":"https://doi.org/10.5376/ijms.2023.13.0003","url":null,"abstract":"","PeriodicalId":22529,"journal":{"name":"The international journal of marine science","volume":"135 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74900797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5376/ijms.2023.13.0002
Jin Wu, L. Jin
{"title":"Classification and Main Characteristics of Marine Ecosystems","authors":"Jin Wu, L. Jin","doi":"10.5376/ijms.2023.13.0002","DOIUrl":"https://doi.org/10.5376/ijms.2023.13.0002","url":null,"abstract":"","PeriodicalId":22529,"journal":{"name":"The international journal of marine science","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84498779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5376/ijms.2023.13.0001
R. Mai
{"title":"Basic Concepts and Practices of Marine Ranching: A Review from Theory to Practice","authors":"R. Mai","doi":"10.5376/ijms.2023.13.0001","DOIUrl":"https://doi.org/10.5376/ijms.2023.13.0001","url":null,"abstract":"","PeriodicalId":22529,"journal":{"name":"The international journal of marine science","volume":"381 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75797652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-26DOI: 10.5376/IJMS.2021.11.0001
S. Wang, Xing Wu, F. Yu, Ting-Cong Liao, Qiongzhen Li, B. Luo, Yan Wang
Paphia amabilis is an important fishery shellfish widely distributing along Southeast Asia coast. The muscles of its foot and siphons are commonly colored bright red-orange due to the presence of C37 skeletal carotenoids. However, 10.5%-18.6% of yellow-muscled individuals (YMs) were found among the four P . amabilis wild samples from Vietnam (Co To island, CT) and China (Beihai, BH/BHA, Guangxi Province; and Zhanjiang, ZJ, Guangdong Province), the north of Beibu Gulf, South China Sea. Twenty genomic microsatellite markers were developed and 12 were employed to analyze the population genetic differentiation of three geographical populations (CT, BH, and ZJ, n=32/population), as well as YM and RM (red-muscled) subpopulations. Medium levels of genetic diversity were detected in all three populations with the number of alleles ranging from 4-21 (8.4±4.5) for CT, 4-16 (7.6±3.3) for BH and 4-18 (7.5±3.7) for ZJ. The average observed/expected heterozygosities were 0.66/0.66 for CT, 0.62/0.63 for BH, and 0.55/0.62 for ZJ, respectively. Total 4 out of 36 tests of microsatellites deviated from Hardy–Weinberg equilibrium (HWE, P <0.05 after Bonferroni correction), all in ZJ population due to the presence of null alleles suggested by MICRO-CHECK. Exact test indicated highly significant differentiations ( F ST ) between Vietnamese and two Chinese populations (0.099/0.123 between CT and BH/ZJ). No allele of any microsatellite marker was found significantly associated with muscularcoloration of P. amabilis , consequently no convergent microsatellite genetic differentiation presents between YM and RM subpopulations, supporting that epigenetic factor might play an important role in carotenoids accumulation of P. amabilis .
{"title":"Muscular Coloration Diversity and Microsatellite Genetic Differentiation for the Clam Paphia amabilis (Philippi, 1847)","authors":"S. Wang, Xing Wu, F. Yu, Ting-Cong Liao, Qiongzhen Li, B. Luo, Yan Wang","doi":"10.5376/IJMS.2021.11.0001","DOIUrl":"https://doi.org/10.5376/IJMS.2021.11.0001","url":null,"abstract":"Paphia amabilis is an important fishery shellfish widely distributing along Southeast Asia coast. The muscles of its foot and siphons are commonly colored bright red-orange due to the presence of C37 skeletal carotenoids. However, 10.5%-18.6% of yellow-muscled individuals (YMs) were found among the four P . amabilis wild samples from Vietnam (Co To island, CT) and China (Beihai, BH/BHA, Guangxi Province; and Zhanjiang, ZJ, Guangdong Province), the north of Beibu Gulf, South China Sea. Twenty genomic microsatellite markers were developed and 12 were employed to analyze the population genetic differentiation of three geographical populations (CT, BH, and ZJ, n=32/population), as well as YM and RM (red-muscled) subpopulations. Medium levels of genetic diversity were detected in all three populations with the number of alleles ranging from 4-21 (8.4±4.5) for CT, 4-16 (7.6±3.3) for BH and 4-18 (7.5±3.7) for ZJ. The average observed/expected heterozygosities were 0.66/0.66 for CT, 0.62/0.63 for BH, and 0.55/0.62 for ZJ, respectively. Total 4 out of 36 tests of microsatellites deviated from Hardy–Weinberg equilibrium (HWE, P <0.05 after Bonferroni correction), all in ZJ population due to the presence of null alleles suggested by MICRO-CHECK. Exact test indicated highly significant differentiations ( F ST ) between Vietnamese and two Chinese populations (0.099/0.123 between CT and BH/ZJ). No allele of any microsatellite marker was found significantly associated with muscularcoloration of P. amabilis , consequently no convergent microsatellite genetic differentiation presents between YM and RM subpopulations, supporting that epigenetic factor might play an important role in carotenoids accumulation of P. amabilis .","PeriodicalId":22529,"journal":{"name":"The international journal of marine science","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82639173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-10-16DOI: 10.5376/IJMS.2020.10.0006
M. Hendri, Oktaviana Lubis Delini, Rozirwan Rozirwan
The species H. micronesica and H. macroloba are a group of green seaweed (Chlorophyta) which are found in the waters of Pulau Maspari. The purposes of this study was to analyze the activity of antioxidant and phytochemicals compounds of the active extract of seaweed H. micronesica and H. macroloba originate from the Maspari Island. The procedures in this study include; collecting and handled samples, stratified maceration both samples using a solvent ethyl acetate (Hmi-EA and Hma-EA) and methanol (Hmi-M and Hma-M), extraction, antioxidants test with DPPH, phytochemical test and analysis IC 50 . The results showed that of the four extracts tested contained an extract that had the ability to inhibit free radicals which extract Hmi-EA. Active extract Hmi-EA had a value of IC 50 52.81 ppm. Active extract Hmi-EA contained alkaloids, steroids and flavonoids. Hmi-EA extract will be highly potential as an antioxidant.
H. micronesica和H. macroloba是一组绿海藻(绿藻),发现于马斯帕里岛的水域。本研究分析了产自马斯帕里岛(Maspari Island)的紫菜H. micronesica和H. macroloba活性提取物的抗氧化活性和植物化学成分的活性。本研究的程序包括;收集和处理样品,使用溶剂乙酸乙酯(Hmi-EA和Hma-EA)和甲醇(Hmi-M和Hma-M)对样品进行分层浸渍,提取,DPPH抗氧化剂测试,植物化学测试和分析IC 50。结果表明,四种提取物中含有一种具有抑制自由基能力的提取物,该提取物可提取Hmi-EA。活性提取物Hmi-EA的ic50为52.81 ppm。活性提取物Hmi-EA含有生物碱、类固醇和黄酮类化合物。Hmi-EA提取物具有很强的抗氧化潜力。
{"title":"Potential Bioactive Compound Seaweed Halimeda micronesica and Halimeda macroloba as Antioxidant and Phytochemical Screening of Active Seaweed Extracts from Maspari Island","authors":"M. Hendri, Oktaviana Lubis Delini, Rozirwan Rozirwan","doi":"10.5376/IJMS.2020.10.0006","DOIUrl":"https://doi.org/10.5376/IJMS.2020.10.0006","url":null,"abstract":"The species H. micronesica and H. macroloba are a group of green seaweed (Chlorophyta) which are found in the waters of Pulau Maspari. The purposes of this study was to analyze the activity of antioxidant and phytochemicals compounds of the active extract of seaweed H. micronesica and H. macroloba originate from the Maspari Island. The procedures in this study include; collecting and handled samples, stratified maceration both samples using a solvent ethyl acetate (Hmi-EA and Hma-EA) and methanol (Hmi-M and Hma-M), extraction, antioxidants test with DPPH, phytochemical test and analysis IC 50 . The results showed that of the four extracts tested contained an extract that had the ability to inhibit free radicals which extract Hmi-EA. Active extract Hmi-EA had a value of IC 50 52.81 ppm. Active extract Hmi-EA contained alkaloids, steroids and flavonoids. Hmi-EA extract will be highly potential as an antioxidant.","PeriodicalId":22529,"journal":{"name":"The international journal of marine science","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86826783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-04DOI: 10.5376/ijms.2020.10.0004
Bess X.Y. Chen, May H.M. Wang
When a whale dies in the ocean, its body slowly sinks to the bottom of the sea. In this process, animals and decomposers gather in the ocean to form an ecosystem. Biologists call this unique process "whale fall." Whale fall, together with ocean cold spring and hydrothermal fluid, is called the "oasis" of the ocean. Whale carcasses provide a rich source of food for all types of consumers in the vast and barren deep-sea food chain, including deep-sea fish, crustaceans, polychaetes and various small organisms. This review introduces the necessary conditions, causes and ecological characteristics of whale fall from various perspectives, and analyzes the impact of whale fall on the ocean; A large number of facts show that whale fall not only provides a huge driving force for the marine carbon flux, but also promotes the production of new species and enriches the diversity of marine organisms. Whale falling is a wonderful creation of nature. It is of great significance to understand this phenomenon for us to understand this unique marine ecosystem.
{"title":"Whale fall: the Creation of A Unique Marine Ecosystem","authors":"Bess X.Y. Chen, May H.M. Wang","doi":"10.5376/ijms.2020.10.0004","DOIUrl":"https://doi.org/10.5376/ijms.2020.10.0004","url":null,"abstract":"When a whale dies in the ocean, its body slowly sinks to the bottom of the sea. In this process, animals and decomposers gather in the ocean to form an ecosystem. Biologists call this unique process \"whale fall.\" Whale fall, together with ocean cold spring and hydrothermal fluid, is called the \"oasis\" of the ocean. Whale carcasses provide a rich source of food for all types of consumers in the vast and barren deep-sea food chain, including deep-sea fish, crustaceans, polychaetes and various small organisms. This review introduces the necessary conditions, causes and ecological characteristics of whale fall from various perspectives, and analyzes the impact of whale fall on the ocean; A large number of facts show that whale fall not only provides a huge driving force for the marine carbon flux, but also promotes the production of new species and enriches the diversity of marine organisms. Whale falling is a wonderful creation of nature. It is of great significance to understand this phenomenon for us to understand this unique marine ecosystem.","PeriodicalId":22529,"journal":{"name":"The international journal of marine science","volume":"98 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83653574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-30DOI: 10.5376/ijms.2020.10.0001
Aroloye O. Numbere
This study is based on the hypothesis that the burrowing habit of fiddler crabs in polluted environment will predispose them to heavy metal contamination. The study was carried out in Eagle Island, a mangrove community. Thirty fiddler crabs ( Uca tangeri ) were captured by hand and butchered into parts and oven dried at 70°C for 48 hours. They were sent to laboratory to determine total hydrocarbon content (THC), Cadmium (Cd), Zinc (Zn) and Lead (Pb) concentrations. The laboratory procedure involved measurement by spectrophotometric method using the HACH DR 890 colorimeter (wavelength 420 nm) for heavy metals and microwave accelerated reaction system (MARS Xpress, North Carolina) for THC. The result indicate that there was no significant difference in THC and heavy metal concentration between crab parts (F 6, 49 = 0.32, P=0.925). However, Zinc had the highest concentration in the crab parts followed by Lead and Cadmium. Zinc was the highest in body tissue (83.57±17.04) mg/L followed by intestine (70.59±1.54) mg/L and ventral shell (67.44±1.1) mg/L. Lead was highest in ventral shell (44.5±34.5) mg/L while Cadmium was highest in carapace (10.02±3.99) mg/L. The order of concentration in the body parts of U. tangeri is Zn>Pb>Cd>THC. THC and Lead were higher in males than in females while Zinc and Cadmium were higher in females. External parts have higher THC, Cd and Pb while internal parts have higher Zn. This result indicates that heavy metals bioaccumulated in fiddler crabs, which can get biomagnified in humans if consumed.
本研究基于招潮蟹在受污染环境中的穴居习性会使其易受重金属污染的假设。这项研究是在红树群落鹰岛进行的。手捕招潮蟹30只,切成部分,70℃烘箱干燥48小时。他们被送到实验室,以测定总碳氢化合物含量(THC),镉(Cd),锌(Zn)和铅(Pb)浓度。实验程序包括使用HACH DR 890比色计(波长420 nm)分光光度法测量重金属和微波加速反应系统(MARS express, North Carolina)测量四氢大麻酚。结果表明,不同部位的四氢大麻酚和重金属含量差异不显著(f6, 49 = 0.32, P=0.925)。蟹体中锌的含量最高,其次是铅和镉。锌含量最高的是体组织(83.57±17.04)mg/L,其次是肠(70.59±1.54)mg/L,腹壳(67.44±1.1)mg/L。腹壳中铅含量最高(44.5±34.5)mg/L,甲壳中镉含量最高(10.02±3.99)mg/L。丹参体内各部位的浓度顺序为Zn b> Pb b>Cd b> THC。四氢大麻酚和铅在男性中高于女性,锌和镉在女性中高于女性。外置部分THC、Cd、Pb含量较高,内置部分Zn含量较高。这一结果表明,重金属在招潮蟹体内的生物积累,如果食用,会在人体中被生物放大。
{"title":"Total Hydrocarbon and Heavy Metal Concentrations in Body Parts of Fiddler Crab (Uca tangeri) (Ocipodidae) in the Niger Delta, Nigeria","authors":"Aroloye O. Numbere","doi":"10.5376/ijms.2020.10.0001","DOIUrl":"https://doi.org/10.5376/ijms.2020.10.0001","url":null,"abstract":"This study is based on the hypothesis that the burrowing habit of fiddler crabs in polluted environment will predispose them to heavy metal contamination. The study was carried out in Eagle Island, a mangrove community. Thirty fiddler crabs ( Uca tangeri ) were captured by hand and butchered into parts and oven dried at 70°C for 48 hours. They were sent to laboratory to determine total hydrocarbon content (THC), Cadmium (Cd), Zinc (Zn) and Lead (Pb) concentrations. The laboratory procedure involved measurement by spectrophotometric method using the HACH DR 890 colorimeter (wavelength 420 nm) for heavy metals and microwave accelerated reaction system (MARS Xpress, North Carolina) for THC. The result indicate that there was no significant difference in THC and heavy metal concentration between crab parts (F 6, 49 = 0.32, P=0.925). However, Zinc had the highest concentration in the crab parts followed by Lead and Cadmium. Zinc was the highest in body tissue (83.57±17.04) mg/L followed by intestine (70.59±1.54) mg/L and ventral shell (67.44±1.1) mg/L. Lead was highest in ventral shell (44.5±34.5) mg/L while Cadmium was highest in carapace (10.02±3.99) mg/L. The order of concentration in the body parts of U. tangeri is Zn>Pb>Cd>THC. THC and Lead were higher in males than in females while Zinc and Cadmium were higher in females. External parts have higher THC, Cd and Pb while internal parts have higher Zn. This result indicates that heavy metals bioaccumulated in fiddler crabs, which can get biomagnified in humans if consumed.","PeriodicalId":22529,"journal":{"name":"The international journal of marine science","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85378363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-22DOI: 10.5376/ijms.2020.10.0002
Yong-ping Su, Jianmin Ye, Qi Liu, Yetao Zeng, Chengshun Zhang, Qingheng Wang
Cell division cycle 25 (Cdc25) is an important dual specificity phosphatase, which plays an important role in regulating the process of oocyte meiosis and embryo development. In this study, the full-length cDNA of Sn-Cdc25 was cloned from S. nudus using RACE technology. The results show that Sn-Cdc25 is 4 130 bp in length, including 3′ UTR 1 849 bp and 5′ UTR 427 bp. The Open reading frame (ORF) is 1 854 bp and encodes 617 amino acids. Sequence analysis shows that the molecular weight of Sn-Cdc25 protein is 69.58 kD, with two typical Cdc25 protein domains: M-phase inducer phosphatase domain and Rhodanese-like domain, and the active site sequence HCX 5 R that can catalyze the dephosphorylation process. Multi-sequence alignment finds that the C-terminal homology is higher than the N-terminal. The tertiary structure prediction shows that the spatial conformation of Cdc25 homologous protein and its active site are highly conservative. A total of 5 Motifs are found in Motif analysis, of which Motif 1 and Motif 2 are Paxillin LD motif and MYND domain binding motif, respectively. Phylogenetic tree analysis shows that Cdc25 is clustered into two branches: invertebrates and vertebrates. RT-PCR results show that the expression of Sn-Cdc25 , with two peaks, is significantly different in different developmental stages of oocytes. The increase in the expression of Sn-Cdc25 from primary vitellogenic stage to the late of active vitellogenic stage (O1-O3) may be related to the process of Sn-Cdc25 promoting DNA replication. When the oocytes entering metanephridium from coelomic fluid, the rapid rise of Sn-Cdc25 expression may be beneficial to the activation of maturation promoting factor (MPF). The above results have accumulated basic data for further understanding of the developmental mechanism of Sipuncula oocytes and for optimization of artificial breeding techniques.
{"title":"Cloning and Expression Analysis of Cdc25 Gene of Sipunculus nudus in Oocytes","authors":"Yong-ping Su, Jianmin Ye, Qi Liu, Yetao Zeng, Chengshun Zhang, Qingheng Wang","doi":"10.5376/ijms.2020.10.0002","DOIUrl":"https://doi.org/10.5376/ijms.2020.10.0002","url":null,"abstract":"Cell division cycle 25 (Cdc25) is an important dual specificity phosphatase, which plays an important role in regulating the process of oocyte meiosis and embryo development. In this study, the full-length cDNA of Sn-Cdc25 was cloned from S. nudus using RACE technology. The results show that Sn-Cdc25 is 4 130 bp in length, including 3′ UTR 1 849 bp and 5′ UTR 427 bp. The Open reading frame (ORF) is 1 854 bp and encodes 617 amino acids. Sequence analysis shows that the molecular weight of Sn-Cdc25 protein is 69.58 kD, with two typical Cdc25 protein domains: M-phase inducer phosphatase domain and Rhodanese-like domain, and the active site sequence HCX 5 R that can catalyze the dephosphorylation process. Multi-sequence alignment finds that the C-terminal homology is higher than the N-terminal. The tertiary structure prediction shows that the spatial conformation of Cdc25 homologous protein and its active site are highly conservative. A total of 5 Motifs are found in Motif analysis, of which Motif 1 and Motif 2 are Paxillin LD motif and MYND domain binding motif, respectively. Phylogenetic tree analysis shows that Cdc25 is clustered into two branches: invertebrates and vertebrates. RT-PCR results show that the expression of Sn-Cdc25 , with two peaks, is significantly different in different developmental stages of oocytes. The increase in the expression of Sn-Cdc25 from primary vitellogenic stage to the late of active vitellogenic stage (O1-O3) may be related to the process of Sn-Cdc25 promoting DNA replication. When the oocytes entering metanephridium from coelomic fluid, the rapid rise of Sn-Cdc25 expression may be beneficial to the activation of maturation promoting factor (MPF). The above results have accumulated basic data for further understanding of the developmental mechanism of Sipuncula oocytes and for optimization of artificial breeding techniques.","PeriodicalId":22529,"journal":{"name":"The international journal of marine science","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81271581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5376/ijms.2020.10.0005
M. Hendri
Seaweed cultivation in Pahawang Island waters have a serious problem like predators from Baronang fish and sea turtle, strong current and big waves also give big impact for seaweed cultivation at there. Bag nets is one of method who can be a solution for that problems. The aim of this research are want to know about daily growth rate (DGR) and absolute growth rate (AGR) from seaweed E. Spinosum. This research has been done in August – September 2019. The net bags was designed to be strong enough for protect seaweed from predation and strong current at there. The net bags prepared are 56 nets, with initial weight of seaweed 50 grams. The result of this research show the daily growth rate (DGR) was 5.6 % each day and for absolute growth rate (AGR) was 304.9 grams. The growth rate is good enough because growth more than 2% each day.
{"title":"Cultivation of Seaweed E.spinosum in Open Waters Using Bags Methods Around Pahawang Island Waters, South Lampung","authors":"M. Hendri","doi":"10.5376/ijms.2020.10.0005","DOIUrl":"https://doi.org/10.5376/ijms.2020.10.0005","url":null,"abstract":"Seaweed cultivation in Pahawang Island waters have a serious problem like predators from Baronang fish and sea turtle, strong current and big waves also give big impact for seaweed cultivation at there. Bag nets is one of method who can be a solution for that problems. The aim of this research are want to know about daily growth rate (DGR) and absolute growth rate (AGR) from seaweed E. Spinosum. This research has been done in August – September 2019. The net bags was designed to be strong enough for protect seaweed from predation and strong current at there. The net bags prepared are 56 nets, with initial weight of seaweed 50 grams. The result of this research show the daily growth rate (DGR) was 5.6 % each day and for absolute growth rate (AGR) was 304.9 grams. The growth rate is good enough because growth more than 2% each day.","PeriodicalId":22529,"journal":{"name":"The international journal of marine science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83016270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.5376/ijms.2020.10.0003
Fuyu Li, Dandan Chen, Yuanchao Li, Shiyao Li, Jingqiu Hou, Daoru Wang, Yan Wang
By mining the EST and genomic microsatellite sequence resources of Porites species in GenBank, and using interspecific amplification and resequencing, we developed 20 microsatellite markers for the stony coral Porites lutea, meanwhile, validated and excluded 43 microsatellite markers of its symbiotic zooxanthella. These markers of P. lutea were genotyped in one wild population (n=16) from the southwest of Hainan Island fringing reefs. Nineteen of the 20 markers showed polymorphism. The number of alleles was 2~7 (mean 3.5±1.8), and the observed heterozygosity (Ho) and expected heterozygosity (He) were 0.06~0.94 (mean 0.34±0.29) and 0.18~0.83 (mean 0.57±0.20), respectively. Four markers remained deviated from the Hardy-Weinberg Equilibrium (HWE) after multiple comparison correction for Bonferroin (PHWE<0.05). This is the first set of microsatellite markers of P. lutea. They should be useful for the research on the genetic diversity and connectivity of this species and the conservation of endangered coral reef ecosystems and reef-building coral communities in the Indo-Pacific region.
{"title":"Development of Microsatellite Markers for the Hermatypic Coral Porites lutea","authors":"Fuyu Li, Dandan Chen, Yuanchao Li, Shiyao Li, Jingqiu Hou, Daoru Wang, Yan Wang","doi":"10.5376/ijms.2020.10.0003","DOIUrl":"https://doi.org/10.5376/ijms.2020.10.0003","url":null,"abstract":"By mining the EST and genomic microsatellite sequence resources of Porites species in GenBank, and using interspecific amplification and resequencing, we developed 20 microsatellite markers for the stony coral Porites lutea, meanwhile, validated and excluded 43 microsatellite markers of its symbiotic zooxanthella. These markers of P. lutea were genotyped in one wild population (n=16) from the southwest of Hainan Island fringing reefs. Nineteen of the 20 markers showed polymorphism. The number of alleles was 2~7 (mean 3.5±1.8), and the observed heterozygosity (Ho) and expected heterozygosity (He) were 0.06~0.94 (mean 0.34±0.29) and 0.18~0.83 (mean 0.57±0.20), respectively. Four markers remained deviated from the Hardy-Weinberg Equilibrium (HWE) after multiple comparison correction for Bonferroin (PHWE<0.05). This is the first set of microsatellite markers of P. lutea. They should be useful for the research on the genetic diversity and connectivity of this species and the conservation of endangered coral reef ecosystems and reef-building coral communities in the Indo-Pacific region.","PeriodicalId":22529,"journal":{"name":"The international journal of marine science","volume":"1672 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86516137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}