Pub Date : 1994-01-01DOI: 10.14924/DERMATOL.104.743
大西 誉光
{"title":"syringomaおよびclear cell syringomaにおけるケラチン発現の免疫組織化学的検討","authors":"大西 誉光","doi":"10.14924/DERMATOL.104.743","DOIUrl":"https://doi.org/10.14924/DERMATOL.104.743","url":null,"abstract":"","PeriodicalId":22553,"journal":{"name":"The Japanese journal of dermatology","volume":"13 1","pages":"743"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82380532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-01-01DOI: 10.14924/DERMATOL.101.1101
澄乃 中島, 眞治 森岡
{"title":"創傷治癒過程,特に表皮細胞遊走性に及ぼすPA/Plasmin系プロテアーゼおよび各種細胞成長因子の影響について","authors":"澄乃 中島, 眞治 森岡","doi":"10.14924/DERMATOL.101.1101","DOIUrl":"https://doi.org/10.14924/DERMATOL.101.1101","url":null,"abstract":"","PeriodicalId":22553,"journal":{"name":"The Japanese journal of dermatology","volume":"6 1","pages":"1101"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88700864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1991-01-01DOI: 10.14924/DERMATOL.101.1253
裕史 中尾
{"title":"マウスmelanoma転移に果たすlysosome酵素の役割―特に腫瘍と周辺組織およびprotease inhibitorとの相互作用について―","authors":"裕史 中尾","doi":"10.14924/DERMATOL.101.1253","DOIUrl":"https://doi.org/10.14924/DERMATOL.101.1253","url":null,"abstract":"","PeriodicalId":22553,"journal":{"name":"The Japanese journal of dermatology","volume":"89 1","pages":"1253"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85959710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1990-10-01DOI: 10.14924/DERMATOL.100.1183
F. Sakamoto, M. Ito, K. Oguro, T. Kazama, H. Fiziwara, M. Tezuka, Y. Sato
Adenosquamous carcinoma, which arose on the face of a 83-year-old woman was studied ultrastructurally and immunohistochemically. The tumor was composed of squamoid cells and mucinous cells including signet-ring cells. Electron microscopically, the tumor cells developed numerous microvilli on the cell surface, and had tonofilaments, well-developed rough endoplasmic reticulum and secretory granules of a mucinous type in the cytoplasm. The signet-ring cells had a large intracytoplasmic cavity containing fine fibrillar, mucin-like substance. Occasionally observed were intercellular masses, around which the tumor cells formed hemidesmosome-like and basal lamina-like structures. Immunohistochemical staining revealed foci of positive reaction for laminin surrounding the tumor cells. The tumor cells displayed positive stainings for stratified-epithelial keratins, but not for simple-epithelial keratins. From these findings, it was concluded that the present tumor might be a carcinoma of squamous cell origin, showing a mucinous metaplasia.
{"title":"Adenosquamous carcinoma of the skin―電顕的および免疫組織化学的検索―","authors":"F. Sakamoto, M. Ito, K. Oguro, T. Kazama, H. Fiziwara, M. Tezuka, Y. Sato","doi":"10.14924/DERMATOL.100.1183","DOIUrl":"https://doi.org/10.14924/DERMATOL.100.1183","url":null,"abstract":"Adenosquamous carcinoma, which arose on the face of a 83-year-old woman was studied ultrastructurally and immunohistochemically. The tumor was composed of squamoid cells and mucinous cells including signet-ring cells. Electron microscopically, the tumor cells developed numerous microvilli on the cell surface, and had tonofilaments, well-developed rough endoplasmic reticulum and secretory granules of a mucinous type in the cytoplasm. The signet-ring cells had a large intracytoplasmic cavity containing fine fibrillar, mucin-like substance. Occasionally observed were intercellular masses, around which the tumor cells formed hemidesmosome-like and basal lamina-like structures. Immunohistochemical staining revealed foci of positive reaction for laminin surrounding the tumor cells. The tumor cells displayed positive stainings for stratified-epithelial keratins, but not for simple-epithelial keratins. From these findings, it was concluded that the present tumor might be a carcinoma of squamous cell origin, showing a mucinous metaplasia.","PeriodicalId":22553,"journal":{"name":"The Japanese journal of dermatology","volume":"72 1","pages":"1183"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73842427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: We cultured human hair follicle cells (HFC) and epidermal keratinocytes (EK) using mixed collagen membranes as the substrate for the culture, and studied the degree of differentiation of these cells. Morphologically, polygonal epithelioid arrangements were seen in both cultures. However, in the culture of EK compared with HFC, large denucleated cells appeared within shorter periods of culture and stratification of cells were more prominent. In the culture of HFC, keratohyalin granules were not observed and cornified envelope formation was suppressed compared with that in EK. Then it is suggested that HFC and EK are different in the degree of keratinization and of other nature.
{"title":"Hair follicle cellとepidermal keratinocyteの相違点について―培養細胞による検討―","authors":"Ryuichiro Kuwana, Seiji Arase, Yasushi Sadamoto, Katsuyuki Takeda","doi":"10.14924/DERMATOL.100.163","DOIUrl":"https://doi.org/10.14924/DERMATOL.100.163","url":null,"abstract":": We cultured human hair follicle cells (HFC) and epidermal keratinocytes (EK) using mixed collagen membranes as the substrate for the culture, and studied the degree of differentiation of these cells. Morphologically, polygonal epithelioid arrangements were seen in both cultures. However, in the culture of EK compared with HFC, large denucleated cells appeared within shorter periods of culture and stratification of cells were more prominent. In the culture of HFC, keratohyalin granules were not observed and cornified envelope formation was suppressed compared with that in EK. Then it is suggested that HFC and EK are different in the degree of keratinization and of other nature.","PeriodicalId":22553,"journal":{"name":"The Japanese journal of dermatology","volume":"100 1","pages":"163"},"PeriodicalIF":0.0,"publicationDate":"1990-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87905837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1989-08-01DOI: 10.14924/DERMATOL.99.985
T. Komatsu, S. Tajima, T. Nishikawa
In this study, we applied the antibody against the molecular weight 15,000 protein purified from breast cyst fluid (GCDFP-15) to cutaneous tissue, especially to sweat glands. Breast cyst fluid was obtained by needle aspiration from patients with gross cystic disease. DEAE-cellulose column chromatography was performed and followed by SDS-polyacrylamide gel electrophoresis. Antisera against the purified protein were prepared in rabbits. We stained specimens of normal skin by the PAP method. The normal apocrine gland cells were strong positive and eccrine gland dark cells were positive. Eccrine gland clear cells were weak positive or negative. Duct cells of both glands were negative. From these observations, we suggest that this antibody is useful for detecting seromucous cells of sweat glands, including both the apocrine gland cells and eccrine dark cells in the skin.
{"title":"乳腺嚢胞内蛋白（GCDFP-15）に対する抗体を用いた正常汗腺組織の免疫組織化学的検討","authors":"T. Komatsu, S. Tajima, T. Nishikawa","doi":"10.14924/DERMATOL.99.985","DOIUrl":"https://doi.org/10.14924/DERMATOL.99.985","url":null,"abstract":"In this study, we applied the antibody against the molecular weight 15,000 protein purified from breast cyst fluid (GCDFP-15) to cutaneous tissue, especially to sweat glands. Breast cyst fluid was obtained by needle aspiration from patients with gross cystic disease. DEAE-cellulose column chromatography was performed and followed by SDS-polyacrylamide gel electrophoresis. Antisera against the purified protein were prepared in rabbits. We stained specimens of normal skin by the PAP method. The normal apocrine gland cells were strong positive and eccrine gland dark cells were positive. Eccrine gland clear cells were weak positive or negative. Duct cells of both glands were negative. From these observations, we suggest that this antibody is useful for detecting seromucous cells of sweat glands, including both the apocrine gland cells and eccrine dark cells in the skin.","PeriodicalId":22553,"journal":{"name":"The Japanese journal of dermatology","volume":"19 1","pages":"985"},"PeriodicalIF":0.0,"publicationDate":"1989-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82802777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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