首页 > 最新文献

The American journal of pediatric hematology/oncology最新文献

英文 中文
Bone marrow transplantation for sickle cell anemia. 骨髓移植治疗镰状细胞性贫血。
M R Abboud, S M Jackson, J Barredo, J Beatty, J Laver

Purpose: To investigate the role of bone marrow transplantation in patients with severe sickle cell anemia (SCA).

Patients and methods: We have designed a protocol for selecting patients with severe SCA who may benefit from bone marrow transplantation (BMT). On the basis of this protocol, a girl 3 9/12 years of age who had severe recurrent pain crises and splenic dysfunction received a BMT from her brother, who is homozygous for hemoglobin A.

Results: Transplantation resulted in prompt engraftment, followed by durable hematologic and immunologic reconstitution with donor cells. One year after BMT, the patient continued to do well. She did not experience any graft versus host disease, her growth velocity increased, and recovery of splenic function was demonstrated. Since undergoing BMT, she has not experienced any painful crises.

Conclusions: Bone marrow transplantation is an effective therapeutic modality that should be considered in patients with severe SCA.

目的:探讨骨髓移植在重度镰状细胞性贫血(SCA)患者中的作用。患者和方法:我们设计了一个方案来选择可能受益于骨髓移植(BMT)的严重SCA患者。在此方案的基础上,一名患有严重复发性疼痛危机和脾功能障碍的3 /12岁女孩接受了血红蛋白a纯合子的兄弟的BMT。结果:移植导致迅速植入,随后是供体细胞持久的血液学和免疫学重建。BMT后一年,患者继续表现良好。她没有发生任何移植物抗宿主病,生长速度加快,脾脏功能恢复。自从接受BMT以来,她没有经历过任何痛苦的危机。结论:骨髓移植是严重SCA患者应考虑的有效治疗方式。
{"title":"Bone marrow transplantation for sickle cell anemia.","authors":"M R Abboud,&nbsp;S M Jackson,&nbsp;J Barredo,&nbsp;J Beatty,&nbsp;J Laver","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the role of bone marrow transplantation in patients with severe sickle cell anemia (SCA).</p><p><strong>Patients and methods: </strong>We have designed a protocol for selecting patients with severe SCA who may benefit from bone marrow transplantation (BMT). On the basis of this protocol, a girl 3 9/12 years of age who had severe recurrent pain crises and splenic dysfunction received a BMT from her brother, who is homozygous for hemoglobin A.</p><p><strong>Results: </strong>Transplantation resulted in prompt engraftment, followed by durable hematologic and immunologic reconstitution with donor cells. One year after BMT, the patient continued to do well. She did not experience any graft versus host disease, her growth velocity increased, and recovery of splenic function was demonstrated. Since undergoing BMT, she has not experienced any painful crises.</p><p><strong>Conclusions: </strong>Bone marrow transplantation is an effective therapeutic modality that should be considered in patients with severe SCA.</p>","PeriodicalId":22558,"journal":{"name":"The American journal of pediatric hematology/oncology","volume":"16 1","pages":"86-9"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19299164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Overview: bone marrow transplantation in the 1990s. 概述:20世纪90年代的骨髓移植。
R Parkman
{"title":"Overview: bone marrow transplantation in the 1990s.","authors":"R Parkman","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22558,"journal":{"name":"The American journal of pediatric hematology/oncology","volume":"16 1","pages":"3-5"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19299251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bone marrow transplantation for thalassemia. The USA experience. 骨髓移植治疗地中海贫血。美国的经验。
M C Walters, K M Sullivan, R J O'Reilly, F Boulad, J Brockstein, K Blume, M Amylon, F L Johnson, M Klemperer, J Graham-Pole

Purpose: We have reviewed the results of bone marrow transplantation in 30 patients with thalassemia major who were treated in the United States.

Patients and methods: Ten patients who underwent transplantation in Seattle and 20 patients from five other U.S. centers were identified through a survey of the International Bone Marrow Transplant Registry. These transplants were performed between November 1981 and April 1992 in patients with diverse ethnic backgrounds and ranged in age from 6 months to 14 years (median 4.0 years). Twenty-seven of the 30 patients received marrow from a human leukocyte antigen (HLA)-identical sibling or other family member, one patient received HLA-matched marrow from an unrelated donor, and two patients were given haploidentical but HLA-mismatched marrow from a related donor. Cytoreductive (preparative) therapy varied among institutions and pretransplant risk categories. In general, patients were given busulfan (12-24 mg/kg) or dimethylmyleran (5 mg/kg) in combination with cyclophosphamide (120-240 mg/kg). A subset of patients were given total body irradiation (TBI) at a dose of 720 cGy followed by cyclophosphamide (120 mg/kg).

Results: Sixteen of 27 patients (59%) who received marrow from an HLA-identical family member are event-free survivors, with a duration of follow-up ranging from 2 months to > 10 years after transplantation. Six of these 27 patients (22%) had recurrence of thalassemia and five (19%) died. The estimated actuarial rate of thalassemia recurrence was 24% and the rate of event-free survival was 57%. Only one of the three patients who received marrow from HLA-nonidentical or unrelated donors survives event-free. Liver biopsies were not routinely performed before transplant. Thus, classification of patients into Lucarelli risk groups was not possible. A modified risk classification was devised by using liver size and iron status assessed by the regularity of chelation and the serum ferritin level. With use of this classification, there was no significant difference in event-free survival between transplant risk groups.

Conclusions: The findings observed in this small series of patients confirms that thalassemia can be cured with bone marrow transplantation. Although most patients are event-free survivors, a significant number experienced recurrence of their disease. A cooperative multicenter trial of U.S. transplant centers may be necessary to evaluate the use of marrow transplantation for thalassemia and to determine optimal treatment.

目的:我们回顾了在美国接受骨髓移植治疗的30例重度地中海贫血患者的结果。患者和方法:通过国际骨髓移植登记处的一项调查,确定了在西雅图接受移植的10名患者和来自美国其他5个中心的20名患者。这些移植手术于1981年11月至1992年4月进行,患者具有不同的种族背景,年龄从6个月到14岁(中位4.0岁)。在30名患者中,27名患者接受了来自人类白细胞抗原(HLA)相同的兄弟姐妹或其他家庭成员的骨髓,1名患者接受了来自无关供者的HLA匹配的骨髓,2名患者接受了来自相关供者的单倍相同但HLA不匹配的骨髓。细胞减少(准备)治疗因机构和移植前风险类别而异。一般情况下,患者给予丁硫丹(12-24 mg/kg)或二甲酰基胺(5 mg/kg)联合环磷酰胺(120-240 mg/kg)。一部分患者接受720 cGy剂量的全身照射(TBI),随后接受环磷酰胺(120 mg/kg)照射。结果:27例接受hla相同家族成员骨髓移植的患者中有16例(59%)是无事件幸存者,移植后随访时间从2个月到> 10年不等。27例患者中有6例(22%)地中海贫血复发,5例(19%)死亡。估计地中海贫血的精算复发率为24%,无事件生存率为57%。接受hla不相同或无亲缘关系供者骨髓的3名患者中,只有1名存活。移植前未常规行肝活检。因此,将患者分为Lucarelli危险组是不可能的。采用肝大小和铁状态,通过螯合规律和血清铁蛋白水平评估,设计了改良的风险分类。使用这种分类,移植风险组间无事件生存率无显著差异。结论:在这一小部分患者中观察到的结果证实了地中海贫血可以通过骨髓移植治愈。虽然大多数患者是无事件幸存者,但相当多的患者经历了疾病复发。美国移植中心的多中心合作试验可能是必要的,以评估骨髓移植对地中海贫血的应用,并确定最佳治疗方法。
{"title":"Bone marrow transplantation for thalassemia. The USA experience.","authors":"M C Walters,&nbsp;K M Sullivan,&nbsp;R J O'Reilly,&nbsp;F Boulad,&nbsp;J Brockstein,&nbsp;K Blume,&nbsp;M Amylon,&nbsp;F L Johnson,&nbsp;M Klemperer,&nbsp;J Graham-Pole","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>We have reviewed the results of bone marrow transplantation in 30 patients with thalassemia major who were treated in the United States.</p><p><strong>Patients and methods: </strong>Ten patients who underwent transplantation in Seattle and 20 patients from five other U.S. centers were identified through a survey of the International Bone Marrow Transplant Registry. These transplants were performed between November 1981 and April 1992 in patients with diverse ethnic backgrounds and ranged in age from 6 months to 14 years (median 4.0 years). Twenty-seven of the 30 patients received marrow from a human leukocyte antigen (HLA)-identical sibling or other family member, one patient received HLA-matched marrow from an unrelated donor, and two patients were given haploidentical but HLA-mismatched marrow from a related donor. Cytoreductive (preparative) therapy varied among institutions and pretransplant risk categories. In general, patients were given busulfan (12-24 mg/kg) or dimethylmyleran (5 mg/kg) in combination with cyclophosphamide (120-240 mg/kg). A subset of patients were given total body irradiation (TBI) at a dose of 720 cGy followed by cyclophosphamide (120 mg/kg).</p><p><strong>Results: </strong>Sixteen of 27 patients (59%) who received marrow from an HLA-identical family member are event-free survivors, with a duration of follow-up ranging from 2 months to > 10 years after transplantation. Six of these 27 patients (22%) had recurrence of thalassemia and five (19%) died. The estimated actuarial rate of thalassemia recurrence was 24% and the rate of event-free survival was 57%. Only one of the three patients who received marrow from HLA-nonidentical or unrelated donors survives event-free. Liver biopsies were not routinely performed before transplant. Thus, classification of patients into Lucarelli risk groups was not possible. A modified risk classification was devised by using liver size and iron status assessed by the regularity of chelation and the serum ferritin level. With use of this classification, there was no significant difference in event-free survival between transplant risk groups.</p><p><strong>Conclusions: </strong>The findings observed in this small series of patients confirms that thalassemia can be cured with bone marrow transplantation. Although most patients are event-free survivors, a significant number experienced recurrence of their disease. A cooperative multicenter trial of U.S. transplant centers may be necessary to evaluate the use of marrow transplantation for thalassemia and to determine optimal treatment.</p>","PeriodicalId":22558,"journal":{"name":"The American journal of pediatric hematology/oncology","volume":"16 1","pages":"11-7"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19297288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bone marrow transplantation for sickle cell disease. The United States experience. 骨髓移植治疗镰状细胞病。美国的经验。
F L Johnson, W C Mentzer, K A Kalinyak, K M Sullivan, M R Abboud

Purpose: As of June 1992, five patients with sickle cell disease had been treated by matched sibling bone marrow transplantation in the United States.

Patients and methods: Three patients underwent transplantations for complications related to sickle cell disease, two with previous cerebrovascular accidents (CVAs) and one who had had multiple severe vasoocclusive crises. Two patients had other indications for allogeneic bone marrow transplantation: one had acute myeloid leukemia and the other had Morquio's disease. The patients' ages ranged from 3 to 10 years, and four were girls. Ages of the donors ranged from 4 to 13 years; four of the donors were boys and three carried the sickle cell trait. For four patients, the preparative regimen consisted of busulfan and cyclophosphamide given either alone or combined with antithymocyte globulin (ATG). The patient with leukemia was prepared with cyclophosphamide and total body irradiation (TBI). The regimens for prophylaxis of graft-versus-host disease (GVHD) included various combinations of cyclosporine A, methotrexate, and prednisone.

Results: The patient with Morquio's disease failed to engraft but underwent a successful retransplantation from the same donor. All patients eventually demonstrated donor engraftment and the donor's hemoglobin electrophoretic pattern posttransplant. Two patients had moderately severe GVHD of the skin and gastrointestinal tract, which resolved with prednisone therapy. One of these patients developed transient chronic GVHD involving the skin. Other acute complications included mild venoocclusive disease of the liver, central line infection with bacteremias, uterine hemorrhage in one patient, and pseudomonas sepsis in another.

Conclusions: Both patients who underwent transplantation after CVAs have experienced subsequent neurological events. However, with a median follow-up of 16 months (range 8 months to 9.3 years), all patients are surviving in good to excellent clinical condition and appear to have benefitted from treatment by bone marrow transplantation.

目的:截至1992年6月,美国有5例镰状细胞病患者接受了匹配的兄弟姐妹骨髓移植治疗。患者和方法:3例患者因镰状细胞病相关并发症接受移植,2例患者既往有脑血管事故(cva), 1例患者有多次严重的血管闭合性危象。两名患者有异基因骨髓移植的其他适应症:一名患有急性髓性白血病,另一名患有莫基奥氏病。患者年龄从3岁到10岁不等,其中4例为女孩。捐赠者的年龄从4岁到13岁不等;其中4名捐赠者是男孩,3名携带镰状细胞特征。对于4名患者,预备方案包括单用或联合抗胸腺细胞球蛋白(ATG)给药的丁硫凡和环磷酰胺。白血病患者采用环磷酰胺和全身照射(TBI)治疗。预防移植物抗宿主病(GVHD)的方案包括环孢素A、甲氨蝶呤和强的松的各种组合。结果:Morquio病患者移植失败,但成功地从同一供体再次移植。所有患者最终均表现出供体移植和供体移植后的血红蛋白电泳模式。2例患者有皮肤和胃肠道的中重度GVHD,经强的松治疗消退。其中一名患者发展为一过性慢性移植物抗宿主病,并累及皮肤。其他急性并发症包括轻微的肝脏静脉闭塞性疾病,一例伴有细菌血症的中央静脉感染,一例子宫出血,另一例假单胞菌脓毒症。结论:CVAs术后接受移植的两例患者均出现了后续的神经系统事件。然而,中位随访16个月(8个月至9.3年),所有患者的临床状况良好至优异,似乎从骨髓移植治疗中受益。
{"title":"Bone marrow transplantation for sickle cell disease. The United States experience.","authors":"F L Johnson,&nbsp;W C Mentzer,&nbsp;K A Kalinyak,&nbsp;K M Sullivan,&nbsp;M R Abboud","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>As of June 1992, five patients with sickle cell disease had been treated by matched sibling bone marrow transplantation in the United States.</p><p><strong>Patients and methods: </strong>Three patients underwent transplantations for complications related to sickle cell disease, two with previous cerebrovascular accidents (CVAs) and one who had had multiple severe vasoocclusive crises. Two patients had other indications for allogeneic bone marrow transplantation: one had acute myeloid leukemia and the other had Morquio's disease. The patients' ages ranged from 3 to 10 years, and four were girls. Ages of the donors ranged from 4 to 13 years; four of the donors were boys and three carried the sickle cell trait. For four patients, the preparative regimen consisted of busulfan and cyclophosphamide given either alone or combined with antithymocyte globulin (ATG). The patient with leukemia was prepared with cyclophosphamide and total body irradiation (TBI). The regimens for prophylaxis of graft-versus-host disease (GVHD) included various combinations of cyclosporine A, methotrexate, and prednisone.</p><p><strong>Results: </strong>The patient with Morquio's disease failed to engraft but underwent a successful retransplantation from the same donor. All patients eventually demonstrated donor engraftment and the donor's hemoglobin electrophoretic pattern posttransplant. Two patients had moderately severe GVHD of the skin and gastrointestinal tract, which resolved with prednisone therapy. One of these patients developed transient chronic GVHD involving the skin. Other acute complications included mild venoocclusive disease of the liver, central line infection with bacteremias, uterine hemorrhage in one patient, and pseudomonas sepsis in another.</p><p><strong>Conclusions: </strong>Both patients who underwent transplantation after CVAs have experienced subsequent neurological events. However, with a median follow-up of 16 months (range 8 months to 9.3 years), all patients are surviving in good to excellent clinical condition and appear to have benefitted from treatment by bone marrow transplantation.</p>","PeriodicalId":22558,"journal":{"name":"The American journal of pediatric hematology/oncology","volume":"16 1","pages":"22-6"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19297290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The U.S. National Marrow Donor Program. 美国国家骨髓捐赠计划。
H A Perkins, J A Hansen

Purpose: The National Marrow Donor Program (NMDP) of the United States has nearly 650,000 unrelated potential marrow donors in its registry and > 1,225 marrow transplants have been performed.

Patient and methods: In 1991, 43% of patients who requested a search found at least one HLA-A-, -B-, -DR-identical donor in the files. The chance of finding a donor match is much better within one's own ethnic group. The individuals enrolled in the donor file are 67.0% white, 3.8% black, 3.0% Asian, 3.9% Hispanic, and 0.8% Native American. Therefore, patients who belong to ethnic minorities are at an obvious disadvantage in obtaining marrow donors. Because of this deficiency, the program has embarked on an aggressive campaign of recruitment of minority donors.

Results: Reciprocal search agreements with other countries have made another 200,000 potential donors available, but it is not likely that black patients will find help by this route.

Conclusions: Several efforts are being made to speed up the search process and to ensure more accurate definition of identities. These efforts include prospective HLA-DR typing of donors in the file, storage of a sample of frozen blood from each donor to permit class II typing (HLA-DR, -DQ) by DNA techniques, and eliminating the mixed lymphocyte culture test as a requirement for designating a given donor as HLA identical.

目的:美国国家骨髓捐赠计划(NMDP)在其登记中有近65万名无血缘关系的潜在骨髓捐赠者,已经进行了超过1225例骨髓移植。患者和方法:1991年,43%的患者要求搜索至少有一个HLA-A, - b, - dr相同的供体在文件中。在自己的种族中找到匹配的供体的机会要大得多。在捐献档案中登记的个体中,白人占67.0%,黑人占3.8%,亚裔占3.0%,西班牙裔占3.9%,美洲原住民占0.8%。因此,少数民族患者在获得骨髓供体方面处于明显的劣势。由于这方面的不足,该项目开始积极招募少数族裔捐助者。结果:与其他国家的互惠搜索协议使另外20万名潜在的捐赠者得以获得,但黑人患者不太可能通过这种途径获得帮助。结论:正在作出若干努力,以加快搜索过程,确保更准确地确定身份。这些努力包括在档案中对献血者进行前瞻性HLA- dr分型,存储每个献血者的冷冻血液样本,以便通过DNA技术进行II类分型(HLA- dr, -DQ),并取消将混合淋巴细胞培养试验作为指定给定献血者HLA相同的要求。
{"title":"The U.S. National Marrow Donor Program.","authors":"H A Perkins,&nbsp;J A Hansen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>The National Marrow Donor Program (NMDP) of the United States has nearly 650,000 unrelated potential marrow donors in its registry and > 1,225 marrow transplants have been performed.</p><p><strong>Patient and methods: </strong>In 1991, 43% of patients who requested a search found at least one HLA-A-, -B-, -DR-identical donor in the files. The chance of finding a donor match is much better within one's own ethnic group. The individuals enrolled in the donor file are 67.0% white, 3.8% black, 3.0% Asian, 3.9% Hispanic, and 0.8% Native American. Therefore, patients who belong to ethnic minorities are at an obvious disadvantage in obtaining marrow donors. Because of this deficiency, the program has embarked on an aggressive campaign of recruitment of minority donors.</p><p><strong>Results: </strong>Reciprocal search agreements with other countries have made another 200,000 potential donors available, but it is not likely that black patients will find help by this route.</p><p><strong>Conclusions: </strong>Several efforts are being made to speed up the search process and to ensure more accurate definition of identities. These efforts include prospective HLA-DR typing of donors in the file, storage of a sample of frozen blood from each donor to permit class II typing (HLA-DR, -DQ) by DNA techniques, and eliminating the mixed lymphocyte culture test as a requirement for designating a given donor as HLA identical.</p>","PeriodicalId":22558,"journal":{"name":"The American journal of pediatric hematology/oncology","volume":"16 1","pages":"30-4"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19299252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beta-S gene cluster haplotypes modulate hematologic and hemorheologic expression in sickle cell anemia. Use in predicting clinical severity. β - s基因簇单倍型调节镰状细胞性贫血的血液学和血液流变学表达。用于预测临床严重程度。
D R Powars, H J Meiselman, T C Fisher, A Hiti, C Johnson

Purpose: The rate of progression of major organ failure in sickle cell anemia is genetically controlled. It is the direct consequence of the sickle cell-evoked vasculopathy.

Patients and methods: Presence of the beta S gene cluster haplotypes and alpha gene deletions as genetic markers indicate the expected frequency of illness and the risk of end-stage major organ failure. The risk of irreversible soft tissue organ failure is greatest in patients with a Central African Republic (CAR) chromosome, whereas morbidity is consistently lowest in patients with a Senegalese chromosome. Presence of alpha-thalassemia-2 decreases the risk of soft tissue organ failure in all haplotype combinations.

Results: Other laboratory abnormalities, when combined with haplotype and alpha gene status, also predict the risk of clinical morbidity. The mean hemoglobin level (or red blood cell count) is lowest in patients with the most severe clinical manifestations. On the other hand, the platelet count and leukocyte count as well as the plasma fibrinogen level are elevated in the sickest patients. A threshold level of hemoglobin F at 1.2 g/dl (approximately 20% hemoglobin F) decreases the risk of major organ failure and is attained most frequently in those with a Senegalese chromosome. Hemorheologic findings observed during the most stable state of patients with sickle cell anemia indicate two trends: (a) the mean percentage of dense red cells is nearly twice as high in the maximal severity patients as compared with the minimal severity patients; and (b) mean red cell rigidity is greatest in the maximal severity group and least in the minimal severity group. These findings suggest that a greater percentage of dense, poorly deformable red cells are present in sickle cell patients in the genotypic category of maximal severity.

Conclusions: The combination of the beta S gene cluster haplotype and alpha-gene status correlates with both phenotypic laboratory findings (hematologic profile) and morbidity. These associations increase our ability to predict clinical severity and the future risk of major organ failure.

目的:镰状细胞性贫血主要器官功能衰竭的进展速度是遗传控制的。这是镰状细胞引起的血管病变的直接后果。患者和方法:存在β S基因簇单倍型和α基因缺失作为遗传标记表明疾病的预期频率和终末期主要器官衰竭的风险。中非共和国染色体患者发生不可逆软组织器官衰竭的风险最大,而塞内加尔染色体患者的发病率始终最低。在所有单倍型组合中,α -地中海贫血-2的存在降低了软组织器官衰竭的风险。结果:其他实验室异常,当与单倍型和α基因状态相结合时,也可以预测临床发病的风险。在临床表现最严重的患者中,平均血红蛋白水平(或红细胞计数)最低。另一方面,在病情最严重的患者中,血小板计数和白细胞计数以及血浆纤维蛋白原水平升高。血红蛋白F的阈值为1.2 g/dl(约20%血红蛋白F)可降低主要器官衰竭的风险,最常见于具有塞内加尔染色体的患者。在镰状细胞性贫血患者最稳定状态时观察到的血液流变学结果显示了两个趋势:(a)最严重患者的致密红细胞平均百分比几乎是最轻微患者的两倍;(b)最大严重程度组平均红细胞硬度最大,最小严重程度组平均红细胞硬度最小。这些发现表明,在最严重的基因型类型中,镰状细胞患者中存在较大比例的致密、不易变形的红细胞。结论:β S基因簇单倍型和α基因状态的组合与表型实验室结果(血液学特征)和发病率相关。这些关联增加了我们预测临床严重程度和未来主要器官衰竭风险的能力。
{"title":"Beta-S gene cluster haplotypes modulate hematologic and hemorheologic expression in sickle cell anemia. Use in predicting clinical severity.","authors":"D R Powars,&nbsp;H J Meiselman,&nbsp;T C Fisher,&nbsp;A Hiti,&nbsp;C Johnson","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>The rate of progression of major organ failure in sickle cell anemia is genetically controlled. It is the direct consequence of the sickle cell-evoked vasculopathy.</p><p><strong>Patients and methods: </strong>Presence of the beta S gene cluster haplotypes and alpha gene deletions as genetic markers indicate the expected frequency of illness and the risk of end-stage major organ failure. The risk of irreversible soft tissue organ failure is greatest in patients with a Central African Republic (CAR) chromosome, whereas morbidity is consistently lowest in patients with a Senegalese chromosome. Presence of alpha-thalassemia-2 decreases the risk of soft tissue organ failure in all haplotype combinations.</p><p><strong>Results: </strong>Other laboratory abnormalities, when combined with haplotype and alpha gene status, also predict the risk of clinical morbidity. The mean hemoglobin level (or red blood cell count) is lowest in patients with the most severe clinical manifestations. On the other hand, the platelet count and leukocyte count as well as the plasma fibrinogen level are elevated in the sickest patients. A threshold level of hemoglobin F at 1.2 g/dl (approximately 20% hemoglobin F) decreases the risk of major organ failure and is attained most frequently in those with a Senegalese chromosome. Hemorheologic findings observed during the most stable state of patients with sickle cell anemia indicate two trends: (a) the mean percentage of dense red cells is nearly twice as high in the maximal severity patients as compared with the minimal severity patients; and (b) mean red cell rigidity is greatest in the maximal severity group and least in the minimal severity group. These findings suggest that a greater percentage of dense, poorly deformable red cells are present in sickle cell patients in the genotypic category of maximal severity.</p><p><strong>Conclusions: </strong>The combination of the beta S gene cluster haplotype and alpha-gene status correlates with both phenotypic laboratory findings (hematologic profile) and morbidity. These associations increase our ability to predict clinical severity and the future risk of major organ failure.</p>","PeriodicalId":22558,"journal":{"name":"The American journal of pediatric hematology/oncology","volume":"16 1","pages":"55-61"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18517037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ethical issues. How can we distinguish clinical research from innovative therapy? 伦理问题。如何区分临床研究和创新疗法?
J Lantos

The difference between research and innovative therapy is based on the goals rather than the risks or newness of the therapy. The threat to patients from research is not that an untested treatment may be hazardous. Instead, the danger is that the loyalty of their physician may be compromised by the goals of research. In the traditional conception of research, it is assumed that we know what constitutes standard therapy and how effective it is. The goal of research is to compare the effectiveness of an innovation with the standard therapy. However, when rapid progress is being made, it becomes difficult to measure improvements due to introduction of new therapies. It is difficult to determine which of many successful therapies is "best." As a result, rapid progress makes all therapies, including both new ones and old ones, nonvalidated therapies. In such situations, scientific norms about the degree of certainty that we must have in order to judge a therapy as being efficacious are based on the values of the individuals involved; rather than on any value-free statistical or scientific calculations. There are identifiable communities (ethnic groups) that are specifically effected by certain genetic diseases, as well as communities consisting of patients who have certain nongenetic diseases. When these groups (patient advocates) and communities are politically organized, they should be consulted and allowed to participate in the process of devising strategies to evaluate new therapies.

研究和创新疗法之间的区别是基于目标,而不是基于治疗的风险或新颖性。研究对患者的威胁并不是未经测试的治疗可能是危险的。相反,危险的是,他们的医生的忠诚可能会受到研究目标的影响。在传统的研究概念中,假设我们知道什么是标准疗法以及它的有效性。研究的目的是比较创新疗法与标准疗法的有效性。然而,当取得快速进展时,很难衡量由于引入新疗法而取得的改善。在众多成功的治疗方法中,很难确定哪一种是“最好的”。因此,快速发展使得所有疗法,包括新疗法和旧疗法,都成为未经验证的疗法。在这种情况下,为了判断一种治疗是否有效,我们必须拥有的确定性程度的科学规范是基于相关个体的价值观;而不是基于任何无价值的统计或科学计算。有一些可确定的社区(族裔群体)特别受到某些遗传疾病的影响,也有一些由患有某些非遗传疾病的患者组成的社区。当这些团体(患者倡导者)和社区在政治上组织起来时,应该征求他们的意见,并允许他们参与制定评估新疗法的策略的过程。
{"title":"Ethical issues. How can we distinguish clinical research from innovative therapy?","authors":"J Lantos","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The difference between research and innovative therapy is based on the goals rather than the risks or newness of the therapy. The threat to patients from research is not that an untested treatment may be hazardous. Instead, the danger is that the loyalty of their physician may be compromised by the goals of research. In the traditional conception of research, it is assumed that we know what constitutes standard therapy and how effective it is. The goal of research is to compare the effectiveness of an innovation with the standard therapy. However, when rapid progress is being made, it becomes difficult to measure improvements due to introduction of new therapies. It is difficult to determine which of many successful therapies is \"best.\" As a result, rapid progress makes all therapies, including both new ones and old ones, nonvalidated therapies. In such situations, scientific norms about the degree of certainty that we must have in order to judge a therapy as being efficacious are based on the values of the individuals involved; rather than on any value-free statistical or scientific calculations. There are identifiable communities (ethnic groups) that are specifically effected by certain genetic diseases, as well as communities consisting of patients who have certain nongenetic diseases. When these groups (patient advocates) and communities are politically organized, they should be consulted and allowed to participate in the process of devising strategies to evaluate new therapies.</p>","PeriodicalId":22558,"journal":{"name":"The American journal of pediatric hematology/oncology","volume":"16 1","pages":"72-5"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19299256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Experimental therapy of sickle cell disease. Use of hydroxyurea. 镰状细胞病的实验治疗。羟基脲的使用。
S Charache

Purpose: Therapy of sickle cell disease with hydroxyurea is experimental.

Patients and methods: We have begun a randomized blinded clinical trial to determine its clinical utility. The efficacy of this drug is unproved and its risks, which include mutagenesis, teratogenesis and carcinogenesis, are poorly understood. These risks are explicitly stated in our consent forms. A significant number of patients who are asked to enroll refuse to enter the study. This refusal is probably because of individual variations in perception of risk and personal inconvenience, as well as differences in perception of personal benefit. We have a few hints as to which patients are more likely to produce increased amounts of fetal hemoglobin, but our findings do not indicate which patients are most likely to show a good clinical response.

Results: Our study group decided not to treat patients under 18 years of age with hydroxyurea until clinical efficacy of the drug is proved in adults. We have criteria for selecting patients for entry into our ongoing study, but the criteria are based more on study design than on an estimate of present or future severity of the manifestations of sickle cell disease.

Conclusions: Features of our previous study and results of the present trial may be helpful in defining indications for bone marrow transplantation in children with sickle cell disease.

目的:羟基脲治疗镰状细胞病是实验性的。患者和方法:我们已经开始了一项随机盲法临床试验,以确定其临床效用。这种药物的疗效尚未得到证实,其风险,包括诱变、致畸和致癌,也知之甚少。这些风险在我们的同意书中都有明确说明。有相当数量的患者被要求参加这项研究。这种拒绝可能是因为个体对风险和个人不便的感知存在差异,以及对个人利益的感知存在差异。我们有一些提示,哪些患者更有可能产生胎儿血红蛋白量增加,但我们的发现并没有表明哪些患者最有可能表现出良好的临床反应。结果:我们的研究组决定在证实该药物在成人中的临床疗效之前,不使用羟基脲治疗18岁以下的患者。我们有选择患者进入我们正在进行的研究的标准,但这些标准更多地基于研究设计,而不是基于对镰状细胞病目前或未来表现严重程度的估计。结论:我们先前研究的特点和本试验的结果可能有助于确定镰状细胞病儿童骨髓移植的适应症。
{"title":"Experimental therapy of sickle cell disease. Use of hydroxyurea.","authors":"S Charache","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>Therapy of sickle cell disease with hydroxyurea is experimental.</p><p><strong>Patients and methods: </strong>We have begun a randomized blinded clinical trial to determine its clinical utility. The efficacy of this drug is unproved and its risks, which include mutagenesis, teratogenesis and carcinogenesis, are poorly understood. These risks are explicitly stated in our consent forms. A significant number of patients who are asked to enroll refuse to enter the study. This refusal is probably because of individual variations in perception of risk and personal inconvenience, as well as differences in perception of personal benefit. We have a few hints as to which patients are more likely to produce increased amounts of fetal hemoglobin, but our findings do not indicate which patients are most likely to show a good clinical response.</p><p><strong>Results: </strong>Our study group decided not to treat patients under 18 years of age with hydroxyurea until clinical efficacy of the drug is proved in adults. We have criteria for selecting patients for entry into our ongoing study, but the criteria are based more on study design than on an estimate of present or future severity of the manifestations of sickle cell disease.</p><p><strong>Conclusions: </strong>Features of our previous study and results of the present trial may be helpful in defining indications for bone marrow transplantation in children with sickle cell disease.</p>","PeriodicalId":22558,"journal":{"name":"The American journal of pediatric hematology/oncology","volume":"16 1","pages":"62-6"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18517038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing clinical severity in children with sickle cell disease. Preliminary results from a cooperative study. 评估儿童镰状细胞病的临床严重程度合作研究的初步结果。
G L Bray, L Muenz, N Makris, L S Lessin

Purpose: Although it is clear that sickle cell disease is curable with bone marrow transplantation, there are few objective criteria that are helpful in the identification of suitable candidates for this aggressive and potentially life-threatening procedure. This disease is characterized by a highly variable clinical course, and there is a need to intervene with marrow transplant before the onset of disease-mediated chronic organ damage. These factors high-light the need for a clinical severity index that can prospectively identify patients who are at high risk for a turbulent clinical course and a poor prognosis.

Patients and methods: We used the Cooperative Study of Sickle Cell Disease data base to identify features of the disease in early childhood (i.e., < 2 years of age) that are associated either with significant morbidity later in childhood or early mortality. Our study population includes the 1,944 children who entered the study before 12 years of age. Univariate analysis showed that factors associated with the occurrence of cerebrovascular accident (51 patients) include hematocrit, rate of change of pocked red cell count, and polymer fraction at 40% oxygen saturation (PF40). Only low hematocrit was predictive of death in this pediatric cohort (45 disease-related deaths).

Results: Our ability to identify other potential factors that correlate with these outcome measures is limited by their small numbers. Hence, it was necessary to designate a different endpoint whose relationship with various clinical and laboratory parameters could be assessed. To accomplish this, a distribution of acute events, which were defined as any episode of pain or acute chest syndrome, was calculated. Also, the age-specific "expected" event rate, defined as the mean number of events per patient-year of observation, was determined.

Conclusions: The relationship between various aspects of sickle cell disease and high positive deviance from the expected event rate will be assessed in a cohort of 519 children who entered the study prior to 7 months of age and were followed beyond their second birthday.

目的:虽然镰状细胞病是可以通过骨髓移植治愈的,但很少有客观的标准可以帮助确定这种具有侵袭性和潜在威胁生命的手术的合适人选。这种疾病的特点是临床病程变化很大,在疾病介导的慢性器官损伤发生之前,需要进行骨髓移植干预。这些因素突出了对临床严重程度指数的需求,该指数可以前瞻性地识别处于动荡临床过程高风险和预后不良的患者。患者和方法:我们使用镰状细胞病合作研究数据库来确定儿童早期(即< 2岁)与儿童后期显著发病率或早期死亡率相关的疾病特征。我们的研究人群包括1944名12岁前进入研究的儿童。单因素分析显示脑血管意外(51例)发生的相关因素包括红细胞压积、袋状红细胞计数变化率和40%氧饱和度下的聚合物分数(PF40)。在该儿科队列中,只有低血细胞比容可预测死亡(45例疾病相关死亡)。结果:我们识别与这些结果测量相关的其他潜在因素的能力受到数量少的限制。因此,有必要指定一个不同的终点,其与各种临床和实验室参数的关系可以评估。为了做到这一点,计算了急性事件的分布,定义为任何疼痛或急性胸综合征的发作。此外,确定了特定年龄的“预期”事件发生率,定义为每个患者-年观察的平均事件数。结论:将在519名儿童队列中评估镰状细胞病各方面与预期事件高阳性偏差率之间的关系,这些儿童在7个月前进入研究,并随访至两岁以上。
{"title":"Assessing clinical severity in children with sickle cell disease. Preliminary results from a cooperative study.","authors":"G L Bray,&nbsp;L Muenz,&nbsp;N Makris,&nbsp;L S Lessin","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>Although it is clear that sickle cell disease is curable with bone marrow transplantation, there are few objective criteria that are helpful in the identification of suitable candidates for this aggressive and potentially life-threatening procedure. This disease is characterized by a highly variable clinical course, and there is a need to intervene with marrow transplant before the onset of disease-mediated chronic organ damage. These factors high-light the need for a clinical severity index that can prospectively identify patients who are at high risk for a turbulent clinical course and a poor prognosis.</p><p><strong>Patients and methods: </strong>We used the Cooperative Study of Sickle Cell Disease data base to identify features of the disease in early childhood (i.e., < 2 years of age) that are associated either with significant morbidity later in childhood or early mortality. Our study population includes the 1,944 children who entered the study before 12 years of age. Univariate analysis showed that factors associated with the occurrence of cerebrovascular accident (51 patients) include hematocrit, rate of change of pocked red cell count, and polymer fraction at 40% oxygen saturation (PF40). Only low hematocrit was predictive of death in this pediatric cohort (45 disease-related deaths).</p><p><strong>Results: </strong>Our ability to identify other potential factors that correlate with these outcome measures is limited by their small numbers. Hence, it was necessary to designate a different endpoint whose relationship with various clinical and laboratory parameters could be assessed. To accomplish this, a distribution of acute events, which were defined as any episode of pain or acute chest syndrome, was calculated. Also, the age-specific \"expected\" event rate, defined as the mean number of events per patient-year of observation, was determined.</p><p><strong>Conclusions: </strong>The relationship between various aspects of sickle cell disease and high positive deviance from the expected event rate will be assessed in a cohort of 519 children who entered the study prior to 7 months of age and were followed beyond their second birthday.</p>","PeriodicalId":22558,"journal":{"name":"The American journal of pediatric hematology/oncology","volume":"16 1","pages":"50-4"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19299254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Availability of related donors for bone marrow transplantation in sickle cell anemia. 镰状细胞性贫血患者骨髓移植相关供体的可获得性。
W C Mentzer, S Heller, P R Pearle, E Hackney, E Vichinsky

Purpose: To determine who might qualify for allogeneic bone marrow transplantation (BMT), we reviewed the medical records of all 143 patients with sickle cell anemia under the age of 16 years who were registered at our center.

Patients and methods: A total of 135 records were complete and were used to estimate donor availability and disease severity. The mean number of siblings per patient was two, but this number decreased to 0.73 if half-siblings and siblings who had sickle cell anemia were excluded. Probability calculations indicated that a human leukocyte antigen (HLA)-matched sibling donor would be available for only 18% of patients with sickle cell disease.

Results: With regard to clinical severity, if only stroke and chronic debilitating pain are considered criteria for bone marrow transplantation, only 16% of sickle cell patients would qualify, but with use of the broader criteria of the National Collaborative Study, 38% of patients would qualify. However, not all parents will consent to have bone marrow transplantation for their child, and only a minority of patients (18%) will have an HLA-matched sibling donor. Thus, as few as 1-2% of the total population of children with sickle cell anemia will ultimately qualify for marrow transplantation. Increasing the number who can undergo transplantation will require increasing the size of the donor pool.

Conclusions: Search for other therapies not based on marrow transplantation should continue. For the majority of patients with sickle cell disease, these nontransplant treatments offer the best chance for enabling patients to achieve greater longevity and a better quality of life.

目的:为了确定谁有资格进行同种异体骨髓移植(BMT),我们回顾了在我们中心登记的所有143例16岁以下镰状细胞性贫血患者的医疗记录。患者和方法:共有135份完整的记录,用于估计供体可用性和疾病严重程度。每位患者的平均兄弟姐妹数为2个,但如果排除同父异母的兄弟姐妹和患有镰状细胞性贫血的兄弟姐妹,这一数字降至0.73。概率计算表明,只有18%的镰状细胞病患者能找到与人类白细胞抗原(HLA)匹配的同胞供体。结果:关于临床严重程度,如果仅考虑中风和慢性衰弱性疼痛作为骨髓移植的标准,只有16%的镰状细胞患者符合条件,但使用国家合作研究的更广泛标准,38%的患者符合条件。然而,并不是所有的父母都会同意为他们的孩子进行骨髓移植,只有少数患者(18%)会有一个匹配hla的兄弟姐妹供体。因此,只有1-2%的镰状细胞性贫血患儿最终有资格进行骨髓移植。增加能够接受移植的人数将需要增加供体池的规模。结论:应继续寻找非基于骨髓移植的其他治疗方法。对于大多数镰状细胞病患者来说,这些非移植治疗为使患者获得更长的寿命和更好的生活质量提供了最好的机会。
{"title":"Availability of related donors for bone marrow transplantation in sickle cell anemia.","authors":"W C Mentzer,&nbsp;S Heller,&nbsp;P R Pearle,&nbsp;E Hackney,&nbsp;E Vichinsky","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To determine who might qualify for allogeneic bone marrow transplantation (BMT), we reviewed the medical records of all 143 patients with sickle cell anemia under the age of 16 years who were registered at our center.</p><p><strong>Patients and methods: </strong>A total of 135 records were complete and were used to estimate donor availability and disease severity. The mean number of siblings per patient was two, but this number decreased to 0.73 if half-siblings and siblings who had sickle cell anemia were excluded. Probability calculations indicated that a human leukocyte antigen (HLA)-matched sibling donor would be available for only 18% of patients with sickle cell disease.</p><p><strong>Results: </strong>With regard to clinical severity, if only stroke and chronic debilitating pain are considered criteria for bone marrow transplantation, only 16% of sickle cell patients would qualify, but with use of the broader criteria of the National Collaborative Study, 38% of patients would qualify. However, not all parents will consent to have bone marrow transplantation for their child, and only a minority of patients (18%) will have an HLA-matched sibling donor. Thus, as few as 1-2% of the total population of children with sickle cell anemia will ultimately qualify for marrow transplantation. Increasing the number who can undergo transplantation will require increasing the size of the donor pool.</p><p><strong>Conclusions: </strong>Search for other therapies not based on marrow transplantation should continue. For the majority of patients with sickle cell disease, these nontransplant treatments offer the best chance for enabling patients to achieve greater longevity and a better quality of life.</p>","PeriodicalId":22558,"journal":{"name":"The American journal of pediatric hematology/oncology","volume":"16 1","pages":"27-9"},"PeriodicalIF":0.0,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19299250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
The American journal of pediatric hematology/oncology
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1