Pub Date : 2000-02-29DOI: 10.11416/KONTYUSHIGEN1930.69.21
A. Kurioka, M. Yamazaki, H. Hirano
{"title":"Characterization of serine protease inhibitors from wild cocoons of Antheraea yamamai and Bombyx mandarina in comparison with those from Bombyx mori","authors":"A. Kurioka, M. Yamazaki, H. Hirano","doi":"10.11416/KONTYUSHIGEN1930.69.21","DOIUrl":"https://doi.org/10.11416/KONTYUSHIGEN1930.69.21","url":null,"abstract":"","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83444252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-02-29DOI: 10.11416/KONTYUSHIGEN1930.69.63
A. Kurioka, M. Yamazaki, H. Hirano
{"title":"Distribution of trypsin inhibitor in the cocoon floss of the silkworm, Bombyx mori","authors":"A. Kurioka, M. Yamazaki, H. Hirano","doi":"10.11416/KONTYUSHIGEN1930.69.63","DOIUrl":"https://doi.org/10.11416/KONTYUSHIGEN1930.69.63","url":null,"abstract":"","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83274653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-02-29DOI: 10.11416/KONTYUSHIGEN1930.69.39
Y. Sugimura, Akio Uchida, Tomoki Adachi, Haruna Hasegawa, E. Kotani, T. Furusawa
{"title":"Major improvements in gene delivery into mulberry leaf cells by particle inflow gun","authors":"Y. Sugimura, Akio Uchida, Tomoki Adachi, Haruna Hasegawa, E. Kotani, T. Furusawa","doi":"10.11416/KONTYUSHIGEN1930.69.39","DOIUrl":"https://doi.org/10.11416/KONTYUSHIGEN1930.69.39","url":null,"abstract":"","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88241560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-12-28DOI: 10.11416/KONTYUSHIGEN1930.68.491
Zhen-Li Tu, K. Shirai, Rensuke Kanekatsu, K. Kiguchi, Y. Kobayashi, M. Taguchi, Hiroshi Watanabe
{"title":"Effects of local heavy ion beam irradiation on the hemopoietic organs of the silkworm, Bombyx mori","authors":"Zhen-Li Tu, K. Shirai, Rensuke Kanekatsu, K. Kiguchi, Y. Kobayashi, M. Taguchi, Hiroshi Watanabe","doi":"10.11416/KONTYUSHIGEN1930.68.491","DOIUrl":"https://doi.org/10.11416/KONTYUSHIGEN1930.68.491","url":null,"abstract":"","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78304159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-12-28DOI: 10.11416/KONTYUSHIGEN1930.68.515
R. Murakami, A. Shirata
Myrothecium leaf spot of mulberry, caused by Myrothecium roridum was reported in 1994 (TAKAHASHI et al.,1994). M.. roriduyn produces toxins against mulberry(MURAKAMI et al.,1995) and the toxins may be one of the pathogenic factors of the fungus(1VIURAKAMI and SHIRATA, 1998).However, there is no clear evidence of the role of the toxins for the factor in pathogenicity. In this paper, the mutants with a weak pathogenicity were induced by irradiation with ultraviolet rays(UV)to clear the role of toxins in the pathogenicity of M. roriduyn, and discussed the relation between the toxins and pathogenicity. Materials and methods Microorganisms and culture:M. yoriduyn(M9403) was isolated from infected mulberry leaves planted from mulberry field in Kagoshima Prefecture in 1994. M. roridum was incubated on potato sucrose agar medium(PSA:broth made of potato 200g, sucrose 20 g, agar 18 g/1 liter)at 25ーC and abundant spores were formed at 2 weeks after inoculation. The spores were washed with sterilized water 3 times and used.
1994年报道了桑树Myrothecium叶斑病，由Myrothecium roridum引起(TAKAHASHI et al,1994)。M . .roriduyn对桑树产生毒素(MURAKAMI et al.，1995)，毒素可能是桑树真菌的致病因素之一(1VIURAKAMI and SHIRATA, 1998)。然而，没有明确的证据表明毒素在致病因素中的作用。本文利用紫外线照射诱导致病性较弱的突变体，明确毒素在毒霉致病性中的作用，并探讨毒素与致病性的关系。材料与方法微生物与培养:M。从1994年鹿儿岛县桑田种植的病桑叶中分离到yoriduyn(M9403)。在25℃的马铃薯蔗糖琼脂培养基(PSA:马铃薯200g、蔗糖20 g、琼脂18 g/1 l)上培养，接种2周后形成大量孢子。用灭菌水冲洗孢子3次后使用。
{"title":"Mutants of Myrothecium roridum with weak pathogenicity induced by irradiation with ultraviolet rays and toxin production ability","authors":"R. Murakami, A. Shirata","doi":"10.11416/KONTYUSHIGEN1930.68.515","DOIUrl":"https://doi.org/10.11416/KONTYUSHIGEN1930.68.515","url":null,"abstract":"Myrothecium leaf spot of mulberry, caused by Myrothecium roridum was reported in 1994 (TAKAHASHI et al.,1994). M.. roriduyn produces toxins against mulberry(MURAKAMI et al.,1995) and the toxins may be one of the pathogenic factors of the fungus(1VIURAKAMI and SHIRATA, 1998).However, there is no clear evidence of the role of the toxins for the factor in pathogenicity. In this paper, the mutants with a weak pathogenicity were induced by irradiation with ultraviolet rays(UV)to clear the role of toxins in the pathogenicity of M. roriduyn, and discussed the relation between the toxins and pathogenicity. Materials and methods Microorganisms and culture:M. yoriduyn(M9403) was isolated from infected mulberry leaves planted from mulberry field in Kagoshima Prefecture in 1994. M. roridum was incubated on potato sucrose agar medium(PSA:broth made of potato 200g, sucrose 20 g, agar 18 g/1 liter)at 25ーC and abundant spores were formed at 2 weeks after inoculation. The spores were washed with sterilized water 3 times and used.","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87247150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-12-28DOI: 10.11416/KONTYUSHIGEN1930.68.469
R. Murakami, H. Yasui, A. Shirata, A. Kato
Myrothecium roridum Tode: Frides, causing leaf spot of mulberry, produces toxins against mulberry. The fungus produces the toxins not only in lesions on mulberry leaves but also on potato sucrose agar medium. Seven toxins, soluble in ethyl acetate, but not in water, were isolated using high pressure liquid chromatograph. Six of the seven toxins were identified with spectroscopic analysis as trichothecenes, including myrotoxin B and D. The remaining toxin was not a trichothecene. The majority (95.1 %) of the isolated toxins was myrotoxin B, which had the highest toxicity against mulberry in the isolated toxins. When spores of M. roridum except for the toxins were used as inoculum on mulberry leaves, no lesions developed. However, the lesion was induced when the spores of M. roridum were inoculated with myrotoxin B at the concentration of which it was not enough to cause necrosis alone. Therefore, myrotoxin B may be an important role in the pathogenicity of M. roridum.
{"title":"Production of myrotoxin B by Myrothecium roridum isolated from Myrothecium leaf spot of mulberry in Japan","authors":"R. Murakami, H. Yasui, A. Shirata, A. Kato","doi":"10.11416/KONTYUSHIGEN1930.68.469","DOIUrl":"https://doi.org/10.11416/KONTYUSHIGEN1930.68.469","url":null,"abstract":"Myrothecium roridum Tode: Frides, causing leaf spot of mulberry, produces toxins against mulberry. The fungus produces the toxins not only in lesions on mulberry leaves but also on potato sucrose agar medium. Seven toxins, soluble in ethyl acetate, but not in water, were isolated using high pressure liquid chromatograph. Six of the seven toxins were identified with spectroscopic analysis as trichothecenes, including myrotoxin B and D. The remaining toxin was not a trichothecene. The majority (95.1 %) of the isolated toxins was myrotoxin B, which had the highest toxicity against mulberry in the isolated toxins. When spores of M. roridum except for the toxins were used as inoculum on mulberry leaves, no lesions developed. However, the lesion was induced when the spores of M. roridum were inoculated with myrotoxin B at the concentration of which it was not enough to cause necrosis alone. Therefore, myrotoxin B may be an important role in the pathogenicity of M. roridum.","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83126388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-12-28DOI: 10.11416/KONTYUSHIGEN1930.68.521
T. Kako
{"title":"Dyeabilities of chitosan-treated tussah silk dyed with acid, basic and disperse dyes","authors":"T. Kako","doi":"10.11416/KONTYUSHIGEN1930.68.521","DOIUrl":"https://doi.org/10.11416/KONTYUSHIGEN1930.68.521","url":null,"abstract":"","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78821355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-12-28DOI: 10.11416/KONTYUSHIGEN1930.68.507
C. Takabayashi, H. Tsuboi, H. Aizawa, M. Iwadare, E. Miyazaki
{"title":"Simulation and actual reeling results of the cocoon filaments reeling pattern performance on the tube type “Silk shell”","authors":"C. Takabayashi, H. Tsuboi, H. Aizawa, M. Iwadare, E. Miyazaki","doi":"10.11416/KONTYUSHIGEN1930.68.507","DOIUrl":"https://doi.org/10.11416/KONTYUSHIGEN1930.68.507","url":null,"abstract":"","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91172335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-12-28DOI: 10.11416/KONTYUSHIGEN1930.68.455
Yukihiro Sato, Masa Sato, Takayuki Shimizu, Koichi Suzuki
{"title":"Bioassay of an Antifungal Fraction prepared from Larval Hemolymph of the Silkworm, Bombyx mori","authors":"Yukihiro Sato, Masa Sato, Takayuki Shimizu, Koichi Suzuki","doi":"10.11416/KONTYUSHIGEN1930.68.455","DOIUrl":"https://doi.org/10.11416/KONTYUSHIGEN1930.68.455","url":null,"abstract":"","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73477819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1999-12-28DOI: 10.11416/KONTYUSHIGEN1930.68.443
Zhen-Li Tu, Shuhei Yamasaki, K. Shirai, Rensuke Kanekatsu, K. Kiguchi, Y. Kobayashi, M. Taguchi, Hiroshi Watanabe
{"title":"Effects of general and local irradiation of heavy ion beams on the development and morphogenesis of the silkworm, Bombyx mori","authors":"Zhen-Li Tu, Shuhei Yamasaki, K. Shirai, Rensuke Kanekatsu, K. Kiguchi, Y. Kobayashi, M. Taguchi, Hiroshi Watanabe","doi":"10.11416/KONTYUSHIGEN1930.68.443","DOIUrl":"https://doi.org/10.11416/KONTYUSHIGEN1930.68.443","url":null,"abstract":"","PeriodicalId":22665,"journal":{"name":"The journal of sericultural science of Japan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77223990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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