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CYP2A6 activity and its effect on nicotine metabolic kinetics in different metabolic activation systems 不同代谢激活系统中CYP2A6活性及其对尼古丁代谢动力学的影响
Tobacco Science & Technology
Pub Date : 2019-03-12 DOI: 10.16135/J.ISSN1002-0861.2019.0094
Wang Huiting, Li Xiang, Hua Chenfeng, Xu Liangtao, Zhao Ge, Shang Ping-ping, Qiao Liangjun, Zhao Junwei, L. Huimin, Peng Guixin, Xie Fuwei
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引用次数: 0
Influences of cut tobacco width on cut tobacco structure, physical and chemical indexes and sensory quality of cigarette with medium circumference 烟丝宽度对中围卷烟烟丝结构、理化指标及感官品质的影响
Tobacco Science & Technology
Pub Date : 2019-03-07 DOI: 10.16135/J.ISSN1002-0861.2019.0089
Wang Zongying, Shi Jianxin, W. Yonghong, Zhao Min, Zhou Xianli, Song Zuguo, Pan Guangle, Zhang Er-qiang, Zhao Hanwen, Gong Jiahao, Xue Lei, Deng Baoan
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引用次数: 0
烟草 BELL 基因家族鉴定及其温室条件下的表达模式分析 烟草 BELL 基因家族鉴定及其温室条件下的表达模式分析
Tobacco Science & Technology
Pub Date : 2019-03-06 DOI: 10.16135/J.ISSN1002-0861.2019.0082
赵森森 武明珠 王根发 罗朝鹏 王晨 李泽锋 杨军 王中 宋卫娜, Song Weina, 赵森森, Zhao Sensen, 武明珠, Wu Mingzhu, 王根发, Wang Genfa, 罗朝鹏, Luo Zhaopeng, 王晨, Wang Chen, 李泽锋, Li Zefeng, 杨军, Yang Jun, 王中, Wang Zhong
BELL基因是植物中普遍存在的一类基因，其编码的同源异型结构域转录因子参与调控了植物叶片、花序等多个组织的发育过程。为明确烟草BELL基因的结构和功能，通过同源序列比对和功能域分析鉴定了普通烟草BELL基因，结合系统进化和基因表达模式分析对烟草BELL基因的功能进行了初步预测。结果表明，从普通烟草基因组中共鉴定出28个NtBELLs基因，分布于15条不同的染色体上。系统进化分析表明，烟草NtBELLs基因可分为4组，每组参与调控烟草不同的发育过程。NtBELLs基因在6个烟草组织中呈现不同的表达模式，且具有明显的组织特异性。干旱和盐胁迫能显著诱导部分NtBELLs基因的表达，表明该基因家族可能参与了植物抵御非生物胁迫的过程。
BELL基因是植物中普遍存在的一类基因，其编码的同源异型结构域转录因子参与调控了植物叶片、花序等多个组织的发育过程。为明确烟草BELL基因的结构和功能，通过同源序列比对和功能域分析鉴定了普通烟草BELL基因，结合系统进化和基因表达模式分析对烟草BELL基因的功能进行了初步预测。结果表明，从普通烟草基因组中共鉴定出28个NtBELLs基因，分布于15条不同的染色体上。系统进化分析表明，烟草NtBELLs基因可分为4组，每组参与调控烟草不同的发育过程。NtBELLs基因在6个烟草组织中呈现不同的表达模式，且具有明显的组织特异性。干旱和盐胁迫能显著诱导部分NtBELLs基因的表达，表明该基因家族可能参与了植物抵御非生物胁迫的过程。
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引用次数: 0
Effects of deep tillage on soil temperature and humidity, root development and economic traits of flue-cured tobacco 深耕对烤烟土壤温湿度、根系发育及经济性状的影响
Tobacco Science & Technology
Pub Date : 2019-03-01 DOI: 10.16135/J.ISSN1002-0861.2019.0074
Liu Zhixuan, Z. Qing-ming, Rang Zhongwen, Li Juan, L. Yongjun, Tang Chungui, Zhong Yue-feng
{"title":"Effects of deep tillage on soil temperature and humidity, root development and economic traits of flue-cured tobacco","authors":"Liu Zhixuan, Z. Qing-ming, Rang Zhongwen, Li Juan, L. Yongjun, Tang Chungui, Zhong Yue-feng","doi":"10.16135/J.ISSN1002-0861.2019.0074","DOIUrl":"https://doi.org/10.16135/J.ISSN1002-0861.2019.0074","url":null,"abstract":"","PeriodicalId":23146,"journal":{"name":"Tobacco Science & Technology","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79506571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Effects of breakable capsules on filter rod, physical characteristics and mainstream smoke of cigarettes 易碎胶囊对卷烟滤棒、物理特性及主流烟雾的影响
Tobacco Science & Technology
Pub Date : 2019-02-19 DOI: 10.16135/j.issn1002-0861.2019.0057
Z. Zhigang, He Jian, Guo Qiulin, Hou Zhilong, Bao Wenhua, Bao Xudong
{"title":"Effects of breakable capsules on filter rod, physical characteristics and mainstream smoke of cigarettes","authors":"Z. Zhigang, He Jian, Guo Qiulin, Hou Zhilong, Bao Wenhua, Bao Xudong","doi":"10.16135/j.issn1002-0861.2019.0057","DOIUrl":"https://doi.org/10.16135/j.issn1002-0861.2019.0057","url":null,"abstract":"","PeriodicalId":23146,"journal":{"name":"Tobacco Science & Technology","volume":"34 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85057356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
基质匹配溶剂校准-GC-MS/MS法测定卷烟主流烟气B[a]P 基质匹配溶剂校准-GC-MS/MS法测定卷烟主流烟气B[a]P
Tobacco Science & Technology
Pub Date : 2019-02-18 DOI: 10.16135/J.ISSN1002-0861.2019.0056
廖惠云, Liao Huiyun, 王瑞, Wang Rui, 张华, Zhang Hua, 吴洋, Wu Yang, 曹毅, Cao Yi, 张媛, Zhang Yuan, 王晨辉, W. Chenhui
为建立基质匹配溶剂校准-GC-MS/MS法快速测定卷烟主流烟气中B[a]P的方法，卷烟烟气经捕集后，采用振荡提取方式，萃取液直接进行色谱分析。考察了基质匹配溶剂的制备过程，比较了色谱进样方式、质谱参数等条件对目标物出峰情况的影响，优选了样品前处理步骤及样品提取方式。结果表明：①在优化条件下，目标物在1.04~20.8 ng/mL浓度范围内线性良好（R2为0.999 6），加标回收率在94.62%~104.81%之间，日内和日间相对标准偏差（RSDs）分别介于3.13%~3.80%和5.41%~6.94%之间，检出限为0.14 ng/支，定量限为0.46 ng/支，低于国标方法报道值。②18个不同盒标焦油量的细支和常规卷烟分析结果显示，本方法测定结果与现行国标方法基本一致。该方法具有操作简便、耗费溶剂少、灵敏度高等特点，适合于卷烟主流烟气B[a]P释放量的快速测定。
为建立基质匹配溶剂校准-GC-MS/MS法快速测定卷烟主流烟气中B[a]P的方法，卷烟烟气经捕集后，采用振荡提取方式，萃取液直接进行色谱分析。考察了基质匹配溶剂的制备过程，比较了色谱进样方式、质谱参数等条件对目标物出峰情况的影响，优选了样品前处理步骤及样品提取方式。结果表明：①在优化条件下，目标物在1.04~20.8 ng/mL浓度范围内线性良好（R2为0.999 6），加标回收率在94.62%~104.81%之间，日内和日间相对标准偏差（RSDs）分别介于3.13%~3.80%和5.41%~6.94%之间，检出限为0.14 ng/支，定量限为0.46 ng/支，低于国标方法报道值。②18个不同盒标焦油量的细支和常规卷烟分析结果显示，本方法测定结果与现行国标方法基本一致。该方法具有操作简便、耗费溶剂少、灵敏度高等特点，适合于卷烟主流烟气B[a]P释放量的快速测定。
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引用次数: 0
Effect of membrane combination technology on chemical components of tobacco extract 膜组合技术对烟草浸膏化学成分的影响
Tobacco Science & Technology
Pub Date : 2019-02-09 DOI: 10.16135/J.ISSN1002-0861.2019.0060
Zheng Jianyu, Liu Jing, Zhou Guiyuan, Y. Peipei, Dong Gao-feng, Wang Baoxing, Wu Jianlin, L. Meihong, Zhou Xiao, Shang Kechao
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引用次数: 0
Effects of filter ventilation on releases of major nitrogen-containing heterocyclic basic aroma components in mainstream cigarette smoke 过滤嘴通风对主流卷烟烟气中主要含氮杂环碱性香气成分释放的影响
Tobacco Science & Technology
Pub Date : 2019-02-01 DOI: 10.16135/J.ISSN1002-0861.2019.0048
Zhu Xian-yue, Cui Chi, Xie Yu-long, Liu Xingliang, Cai Junlan, Li Huaiqi, Huaping Lei, Tan Tao, Wang Hongbo, Zhang Xiaobing, Zhai Yujun, Peng Guixin
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引用次数: 0
Influences of stem processing on physical properties of cut stems 茎加工对切茎物理性质的影响
Tobacco Science & Technology
Pub Date : 2019-01-31 DOI: 10.16135/j.issn1002-0861.2019.0046
Liu Minchang, Liu Yang, W. Wu, Wen Ruoyu, Kong Zhen, Li Bin, Z. Dabo
{"title":"Influences of stem processing on physical properties of cut stems","authors":"Liu Minchang, Liu Yang, W. Wu, Wen Ruoyu, Kong Zhen, Li Bin, Z. Dabo","doi":"10.16135/j.issn1002-0861.2019.0046","DOIUrl":"https://doi.org/10.16135/j.issn1002-0861.2019.0046","url":null,"abstract":"","PeriodicalId":23146,"journal":{"name":"Tobacco Science & Technology","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75409656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
15 N isotope tracing analysis of nitrogen accumulation and ammonia volatilization in upper leaves of flue-cured tobacco varieties 烤烟品种上部叶片氮素积累和氨挥发的15n同位素示踪分析
Tobacco Science & Technology
Pub Date : 2019-01-29 DOI: 10.16135/j.issn1002-0861.2019.0044
徐清泉, Xu Qingquan, 刘化冰, Liu Huabing, 尚晓颍, Shan Xiaoying, 程昌合, Chen Changhe, 张晓兵, Zhang Xiaobing, 夏琛, Xia Chen, 张勇刚, Zhang Yonggang, 金慧清, Jin Huiqing, 徐世晓, Xu Shixiao, 杨铁钊, Yang Tiezhao, 邹光进, Zou Guangjin
为明确贵州黔东南烟区不同烤烟品种上部叶对土壤氮源和肥料氮源的利用差异，以当地主栽品种云烟87和K326为试验材料，通过施用15N同位素标记的15NH415NO3肥（基肥，70%）和K15NO3肥（追肥，30%）研究了两个品种上部叶的氮素积累和氨挥发的差异性。两个品种烤烟上部叶氮素含量（质量分数）随生育期的推进呈先升高后降低的趋势，两个品种烤烟吸收土壤氮素含量占烟株全氮含量的比例随生育期的推进而逐渐升高，对上部叶的氮素营养贡献率较大；云烟87上部叶氨挥发量大于K326，两个品种烤烟上部叶氨挥发的氮源主要来自肥料氮。黔东南烟区土壤氮对上部叶的氮素营养起主导作用，不同烤烟品种上部叶对土壤氮和肥料氮的利用存在差异。
为明确贵州黔东南烟区不同烤烟品种上部叶对土壤氮源和肥料氮源的利用差异，以当地主栽品种云烟87和K326为试验材料，通过施用15N同位素标记的15NH415NO3肥（基肥，70%）和K15NO3肥（追肥，30%）研究了两个品种上部叶的氮素积累和氨挥发的差异性。两个品种烤烟上部叶氮素含量（质量分数）随生育期的推进呈先升高后降低的趋势，两个品种烤烟吸收土壤氮素含量占烟株全氮含量的比例随生育期的推进而逐渐升高，对上部叶的氮素营养贡献率较大；云烟87上部叶氨挥发量大于K326，两个品种烤烟上部叶氨挥发的氮源主要来自肥料氮。黔东南烟区土壤氮对上部叶的氮素营养起主导作用，不同烤烟品种上部叶对土壤氮和肥料氮的利用存在差异。
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