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Variation in Genetic Diversity in Okra (Abelmoschus esculentus L.) 秋葵遗传多样性的变异
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2017.00008.7
D. K. Saryam, S. Mittra, A. Mehta., S. Prajapati
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引用次数: 1
The Effect of Drought Stress on the Activity of Antioxidant Enzymes of Glycyrrhiza glabra 干旱胁迫对甘草抗氧化酶活性的影响
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2016.00003.2
Vida Nasrollahi, A. Mirzaie-asl, L. Khodaei, S. Jamalian
Drought stress is a crucial factor that affects plant growth and development. Reposition of reactive oxygen species (ROS) is one of the biochemical changes happening when plants are exposed to stress. Plants use enzymatic antioxidant defense mechanisms to remove ROS. In the current study, variations of antioxidant enzymes were measured in leaves and stolons of licorice plants (Glycyrrhiza glabra) growing under normal and drought conditions. Samples were harvested at 2, 16, 26 and 28 days after drought imposition treatment. These samples assigned to three drought treatments, without stress (S2), moderate stress (S16) and severe stress (S26 and S28). In comparison with control samples, superoxide dismutase (SOD), ascorbate peroxidase (APX) and peroxidase (POD) activities were up regulated by moderate and severe drought stress in leaves and stolons. The maximum activities of these enzymes were detected in severe stress. Catalase (CAT) and glutathione reductase (GR) activities were decreased in all stressed samples. The increased activities of SOD, APX and POD in severe stress indicated the important role of these enzymes in protecting licorice plants against oxidative stress.
干旱胁迫是影响植物生长发育的重要因素。活性氧(ROS)的重新定位是植物在逆境中发生的生化变化之一。植物利用酶促抗氧化防御机制去除活性氧。本研究对正常和干旱条件下生长的甘草叶片和匍匐茎中抗氧化酶的变化进行了测定。分别在干旱胁迫处理后的第2、16、26和28天收获样品。这些样品分为无胁迫(S2)、中度胁迫(S16)和重度胁迫(S26和S28) 3个处理。与对照相比,中重度干旱胁迫显著上调了叶片和匍匐茎超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)和过氧化物酶(POD)活性。这些酶的活性在严重胁迫下达到最大值。过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性均降低。SOD、APX和POD在严重胁迫下活性升高,表明这些酶在保护甘草抗氧化胁迫中的重要作用。
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引用次数: 1
Plant Breeding and Secondary Metabolite Overviews 植物育种与次生代谢物综述
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2017.00013.0
Nahida Tun Nisa, Z. Kaloo, S. Qadir
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引用次数: 0
Studies on Frequency of Chlorophyll and Morphological Mutants in Chickpea 鹰嘴豆叶绿素和形态突变频率的研究
Pub Date : 1900-01-01 DOI: 10.5958/J.2231-1742.2.1.007
Mehrajuddin Bhat, Samiullah Khan, M. I. Kozgar
The effect of potent mutagenic chemicals, ethylmethane sulphonate (EMS), sodium azide (SA) and hydrazine hydrate (HZ), on two varieties, namely Avrodhi and BG- 256 of chickpea has been described in relation to chlorophyll and morphological mutations induced in the M2 generation. The frequency of mutations in terms of total plant basis was found to be higher at a lower concentration and concomitantly decreased with the increasing concentrations of EMS, whereas in SA and HZ it does not show any definite trend. Spectrum of chlorophyll mutations included xantha type followed by chlorina type, which outnumbered other types, namely albino, tigrina, and viridis in both the varieties, whereas the morphological mutants include changes for plant height, growth habit, foliage, pod and seed size, flowers, flowering, and maturity period. Frequency of mutations on variety basis indicated that both varieties were found to respond to the mutagenic treatments differently and on mutagen basis show relative specificity. The mutagenized population provides invaluable material for selecting types with the ideal combination of desirable features.
本文报道了强诱变化学物质甲烷磺酸乙酯(EMS)、叠氮化钠(SA)和水合肼(HZ)对2个鹰嘴豆品种Avrodhi和BG- 256在M2代诱导的叶绿素和形态突变的影响。在全株基础上,突变频率在较低浓度下较高,并随EMS浓度的增加而降低,而在SA和HZ中则没有明显的变化趋势。叶绿素突变谱以黄原型居首,其次是绿原型,白化型、绿原型和绿原型居首,形态突变谱包括株高、生长习性、叶片、荚果和种子大小、花、开花和成熟期的变化。突变频率在品种基础上表明两个品种对诱变处理的反应不同,在诱变原基础上表现出相对特异性。诱变群体为选择具有理想特征组合的品种提供了宝贵的材料。
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引用次数: 2
Impacts of Some Biostimulants on the Phenolic Synthesis and Furanochromones Content in Ammi visnaga L. 几种生物刺激剂对虾蛄酚类合成及呋喃酮含量的影响。
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2017.00009.9
H. Khattab, I. Talaat, Aisha Ahmed
The traditional medicinal Ammi visnaga L. plants can be considered as an important economic source of raw materials for the drug industry. Nowadays, increasing the drug raw materials by using safe and favourable treatments is an important issue. So searching for the proper concentration of the suitable bioregulators which achieving high growth rate and enhancing chemical constituents of A. visnaga plants is the goal of this investigation. Foliar application of different concentrations of eitheramino acids (tyrosine and phenylalanine) and phenolic acids (trans-cinnamic acid, benzoic acid and salicylic acid) displayed significant positive effects on the accumulation of chemical constituents including phenolic compounds, flavonoids and total furanochromones in A. visnaga L. Moreover, the high-performance liquid chromatography analysis showed that some phenolic acids are highly increased, decreased or disappear in A. visnaga plants treated with various concentrations of amino acids and phenolic compounds as being compared with untreated control plants. Ferulic acid, cinnamic acid, coumaric acid and P-hydroxy benzoic acid are the most dominant phenolics in Ammi plants. Phenolics are more effective in stimulating the accumulation of phenols, flavonoids, chromones and consequently the scavenging capacity compared with the investigated aromatic amino acids. Such stimulatory effects are positively related to the applied investigated concentrations of the applied bioregulators. Indeed, Ammi plants treated with phenolics particularly benzoic acid (20 mg L−1) exhibited the greatest accumulated levels of phenolics and chromones there by enhancing thee conomic source of raw materials for drug industry.
传统药用植物阿米(Ammi visnaga L.)可被认为是制药业重要的原料经济来源。目前,利用安全、有利的处理方法来增加药物原料是一个重要的问题。因此,寻找合适的生物调节剂浓度,既能提高麻花的生长速度,又能提高麻花的化学成分,是本研究的目标。叶面施用不同浓度的氨基酸(酪氨酸和苯丙氨酸)和酚酸(反式肉桂酸、苯甲酸和水杨酸)对香姜中酚类化合物、黄酮类化合物和总呋喃醌等化学成分的积累有显著的正向影响,且高效液相色谱分析表明,部分酚酸含量显著增加;与未处理的对照植物相比,不同浓度的氨基酸和酚类化合物处理后的麻花草植株的氨基酸含量降低或消失。阿魏酸、肉桂酸、香豆酸和对羟基苯甲酸是阿米属植物中最主要的酚类物质。与所研究的芳香氨基酸相比,酚类物质更有效地刺激酚类、黄酮类和色素的积累,从而提高清除能力。这种刺激效应与应用研究的应用生物调节剂浓度呈正相关。事实上,经苯甲酸(20 mg L−1)处理的蜜属植物的酚类物质特别是苯甲酸(20 mg L−1)积累的酚类物质和色素含量最高,从而增加了制药厂原料的三个经济来源。
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引用次数: 3
Morphological and Anatomical Effects of the Herbicide Roundup on Zea mays (Poaceae) 除草剂农达对玉米(禾科)形态和解剖的影响
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2014.00003.1
Burçak Tütünoglu, Özlem Aksoy
The toxicity of a widely used herbicide Roundup, which contains the active ingredient glyphosate, on sweet corn (Zea mays) was investigated. After the determination of EC50 value for root growth, three different concentrations as low (4 ml/L), medium (8 ml/L) and high dose (16 ml/L) were applied to plant samples for 48 and 72 h. At the end of the application period, samples were comparatively studied in terms of their root and leaf anatomy, the upper and lower surface of leaf stomata indexes as well as chlorophyll contents to obtain morphological and anatomical information. Each concentration of glyphosate caused severe toxic effects on Zea mays cells and the strongest toxic effect was observed at the concentration level of 16 ml/L. At the end of the application procedure, the measurements of the parts of the Z.mays root treated with Roundup, the upper and lower surface of leaf stomata indexes, chlorophyll and total carotenoid concentrations were determined in root apical meristematic cells by using squashing techniques.
研究了广泛使用的草甘膦除草剂农达对甜玉米(Zea mays)的毒性。测定根系生长的EC50值后,将低浓度(4 ml/L)、中浓度(8 ml/L)和高剂量(16 ml/L) 3种浓度分别施用于植物样品48和72 h。施用期结束时,对样品的根、叶解剖、叶片上、下表面气孔指数以及叶绿素含量进行对比研究,获取形态解剖信息。各浓度草甘膦对玉米细胞均有较强的毒性作用,在浓度为16 ml/L时毒性作用最强。在施用过程结束时,采用压扁技术测定了农达处理的玉米根各部位、叶片上、下表面气孔指数、根尖分生组织细胞中叶绿素和总类胡萝卜素浓度。
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引用次数: 1
Population and Phytosociological Patterns of Two Different Altitudes with Emphasis on Medicinal Plants in Garhwal Himalaya 以药用植物为重点的加尔瓦尔-喜马拉雅地区两种不同海拔的种群和植物社会学格局
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2017.00014.2
A. Bisht
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引用次数: 0
High Frequency Clonal Multiplication of Stevia rebaudiana Bertoni, Sweetener of the Future 甜菊糖的高频克隆繁殖,未来甜味剂
Pub Date : 1900-01-01 DOI: 10.5958/J.2231-1742.1.1.007
Shiwali Sharma, A. Shahzad
The present study describes an efficient, rapid clonal propagation protocol for a natural sweetener herb, Stevia rebaudiana. Shoot tips and nodal segments were inoculated on Murashige and Skoog’s medium [MS 1962] containing different cytokinin and auxin alone or in combinations. Shoot tips were proved to be better in comparison to nodal segments having higher rate of shoot induction and multiplication. Best shoot multiplication from both the explants was obtained on MS medium supplemented with 5.0 µM 6benzyladenine (BA) and 1.0 µM a-naphthalene acetic acid (NAA). A maximum of 19.60 shoots per shoot tip and 16.00 shoots per nodal segment with an average shoot length of 4.70 and 4.28 cm were obtained. Subculturing of the regenerating tissue on the optimized treatment showed further enhancement in shoot proliferation without any decline in multiplication rate. The regenerated microshoots showed the most efficient rooting on half strength MS medium augmented with 2.5 µM indole-3-butyric acid (IBA). Plantlets went through a hardening phase prior to ex vitro transfer and established in earthen pots containing garden soil and green manure (1:1). The established plantlets were uniform and identical to mother plant with respect to growth characteristics and vegetative morphology.
本研究描述了一种高效,快速克隆繁殖方案的天然甜味剂草药,甜菊糖。茎尖和节段分别接种于单独或联合含有不同细胞分裂素和生长素的Murashige和Skoog培养基[MS 1962]上。茎尖比节段具有更高的诱导增殖率。两种外植体在添加5.0µM 6benzyladenine (BA)和1.0µM a-萘乙酸(NAA)的MS培养基上增殖效果最好。每个茎尖和节段的芽数分别为19.60和16.00个,平均芽长分别为4.70和4.28 cm。经优化处理的再生组织继代培养,茎部增殖能力进一步增强,但增殖率没有下降。再生微芽在添加2.5µM吲哚-3-丁酸(IBA)的半强度MS培养基上生根效果最好。试管苗在体外移植前经历了一个硬化阶段,并在含有花园土壤和绿肥(1:1)的陶罐中培养。建立的植株在生长特征和营养形态上与母株一致。
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引用次数: 3
Elucidating the Dynamics of Physiology and Essential Oil Production in Lemongrass Using Alginate Oligomers under Field Conditions 利用海藻酸酯低聚物在田间条件下研究柠檬草的生理动态和精油生产
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2017.00004.X
Hassan Jaleel, M. A. Khan, B. Ahmad, A. Shabbir, Y. Sadiq, M. Uddin, L. Varshney
Cymbopogon flexuosus (Steud.) Wats. (lemongrass) essential oil (EO) is valued for its aromatic and medicinal properties as it holds an important share among volatile oils traded worldwide and exhibits massive diversity in commercial and consumer utility. The last decade witnessed several pot experiments to test the ability of radiation-processed sodium alginate as a plant growth elicitor. Therefore, the present work was designed to authenticate the pot experiment findings and the study was carried out in the field (30 × 30 m2), according to the simple randomised block design at Agricultural Farm, Aligarh Muslim University, Aligarh, UP (India). Different foliar concentrations of irradiated sodium alginate (ISA; 0 (control), 40, 60, 80 and 100 mg L–1) were applied to scrutinise the performance of lemongrass with regard to ISA under field conditions. The effect of ISA, at a given concentration range of 40–100 mg L–1, was found significant in terms of growth, physiological and biochemical as well as quality attributes. However, ISA-60 mg L–1 proved most favourable and substantially enhanced the activities of carbonic anhydrase and nitrate reductase by 18.4 and 15.1%, respectively and chlorophyll fluorescence by 12.8% in comparison to control. Further, due to applied treatment, the content and yield of EO was increased by 35.6 and 91.4%, respectively, over control. Moreover, citral yield was also enriched by 90.4% over control due to ISA applied at 60 mg L–1.
柔顺银脚龙寺庙。(柠檬草)精油(EO)因其芳香和药用特性而受到重视,因为它在世界范围内交易的挥发油中占有重要份额,并在商业和消费用途中表现出巨大的多样性。过去十年见证了几个盆栽实验,以测试辐射处理的海藻酸钠作为植物生长促进剂的能力。因此,本研究旨在验证盆栽试验结果,并根据简单随机区组设计在印度北方邦阿里加尔穆斯林大学农业农场(30 × 30 m2)进行。辐照海藻酸钠(ISA)叶片浓度的变化;分别用0(对照)、40、60、80和100 mg L-1,考察柠檬草在田间条件下的ISA性能。在40 ~ 100 mg L-1的浓度范围内,对玉米的生长、生理生化和品质性状均有显著影响。其中,ISA-60 mg L-1处理效果最好,与对照相比,碳酸酐酶和硝酸还原酶活性分别提高了18.4%和15.1%,叶绿素荧光活性提高了12.8%。此外,由于施用处理,EO的含量和产量分别比对照提高了35.6%和91.4%。此外,在60 mg L-1的辐照条件下,柠檬醛的产量比对照提高了90.4%。
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引用次数: 4
Enhancement of Growth and Yield of Mustard (Brassica Juncea L.) Var. Varuna by Thiamine Hydrochloride (Vitamin-B1) Application 促进芥菜(Brassica Juncea L.)生长和产量盐酸硫胺素(维生素b1)的应用
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2015.00004.9
Azhar Sajjad, Samiullah
One field experiment was conducted according to simple randomised block design on mustard (Brassica juncea L.) Var. Varuna to establish the most suitable concentration for soaking of mustard seeds in thiamine hydrochloride solutions of concentrations, that is, 0.01, 0.02, 0.03, 0.06, 0.09 and 0.12% on the performance of mustard. The parameters such as plant dry weight, leaf area, leaf area index (LAI), crop growth rate (CGR), relative growth rate (RGR) and net assimilation rate (NAR) were calculated from 40 to 120 days after sowing (DAS). Yield and its components such as number of pods per plant, 1000 seed weight, yield; biological yield, harvest index and oil yield were measured at harvest. In general soaking of seeds in 0.03% of thiamine hydrochloride solution was found to be the best in comparison to any other concentration of the treatment for most of the parameter studied. In growth parameters, leaf area, plant dry weight showed significant results at the early stages of sampling (that is, 40–80 DAS). CGR also followed the same trend; however, RGR and NAR did not follow the same at any stage of the sampling. Yield parameters like the pod number per plant, biological yield and seed yield as well as oil yield registered significant result. Thus, it was concluded that soaking of mustard seeds in 0.03% thiamine hydrochloride ensured better growth and yield.
采用简单随机区组设计对芥菜(Brassica juncea L.)进行田间试验。以Varuna . Varuna为研究对象,确定了在盐酸硫胺素溶液中浸泡芥菜籽的最适宜浓度,即0.01、0.02、0.03、0.06、0.09和0.12%对芥菜性能的影响。在播后40 ~ 120 d计算植株干重、叶面积、叶面积指数(LAI)、作物生长率(CGR)、相对生长率(RGR)和净同化率(NAR)等参数。产量及其构成要素,如单株荚果数、千粒重、产量;收获时测定生物产量、收获指数和油脂产量。总的来说,与其他任何浓度的处理相比,在0.03%盐酸硫胺素溶液中浸泡种子对大多数参数的影响是最好的。在生长参数、叶面积、植株干重方面,取样初期(即40-80 DAS)表现出显著的结果。CGR也遵循同样的趋势;然而,RGR和NAR在抽样的任何阶段都没有遵循相同的方法。单株荚果数、生物产量、种子产量、油料产量等产量参数均取得显著成果。由此可见,在0.03%盐酸硫胺素中浸泡芥菜籽能获得较好的生长和产量。
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引用次数: 3
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Journal of Functional and Environmental Botany
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