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Studies on the Biochemical Composition of Parthenium hysterophorus L. in Different Seasons 不同季节子宫草的生化成分研究
Pub Date : 1900-01-01 DOI: 10.5958/J.2231-1742.2.2.014
P. K. Mishra, B. Maurya, Prasann Kumar
During 2006 Parthenium hysterophorus L. plant material was collected from the Agricultural Research Farm, B.H.U. during the rainy and winter season, to investigate changes in content of the some biochemical compounds in different plant parts (root, stem and leaf). High content of both protein and starch was recorded in leaves in both seasons. The content of reducing sugars and carbohydrates were higher during rainy season in all parts of Parthenium as compared to the winter season, while non- reducing sugars were highest in leaf material during the rainy season as compare to winter season.
2006年,在丰水季和冬季,在北京农业研究农场采集了宫草(Parthenium hysterophorus L.)植物材料,研究了不同植物部位(根、茎、叶)某些生化化合物含量的变化。两个季节的叶片蛋白质和淀粉含量都很高。雨季各部位的还原糖和碳水化合物含量均高于冬季,而叶片中非还原糖含量在雨季均高于冬季。
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引用次数: 27
Callus, as a Prospective Source of Proficient Cells for Determining Phenolic Contents and Antioxidant Potential in Clitoria ternatea L. 愈伤组织作为测定阴蒂酚类物质含量和抗氧化潜能的潜在细胞来源。
Pub Date : 1900-01-01 DOI: 10.5958/J.2231-1750.3.2.009
Najma Ismail, R. N. Verma, A. Batra
A potential medicinal legume Clitoria ternatea L., commonly known as butterfly pea, has been investigated for determining phenolic contents and antioxidant potential. In the present study, callus was used as a prospective source of proficient cells obtained from leaf explants of C. ternatea L. Maximum callus was obtained by Murashige and Skoog, 1962 (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2.0 mg/l). The different in vivo and in vitro (callus) extracts, viz., ethanolic, acetone, petroleum ether, were used for total phenolic contents and antioxidant activity. The total phenol varied from mg/g Distilled Water (dw) in the various extracts was screened for their free-radical scavenging properties using ascorbic acid as standard antioxidant. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging effect of the extracts was determined spectrophotometrically. The highest radical scavenging effect was observed in in vitro acetone extracts with IC50%: 24.4±0.34 μg/ml. The greater amount of phenolic compounds leads to more potent radical scavenging effect as shown by the same extract of C. ternatea L.
本文研究了一种具有药用潜力的豆科植物,俗称蝶豆,其酚类物质含量和抗氧化能力。在本研究中,愈伤组织被作为一种潜在的成熟细胞来源,从三叶参(C. ternatea l .)的叶片外植体中获得,在Murashige和Skoog, 1962 (MS)培养基中添加2,4-二氯苯氧乙酸(2.0 mg/l),获得最多的愈伤组织。用乙醇、丙酮、石油醚等不同的愈伤组织提取物测定其总酚含量和抗氧化活性。以抗坏血酸为标准抗氧化剂,对不同提取物中总酚(mg/g蒸馏水,dw)的清除自由基性能进行了筛选。分光光度法测定了各提取物对1,1-二苯基-2-苦酰肼自由基的清除作用。体外清除自由基效果最好的是丙酮提取物,IC50%为24.4±0.34 μg/ml。相同提取物中酚类化合物含量越高,自由基清除作用越强。
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引用次数: 0
Food Security and Nutritional Safety: A Challenge Ahead 粮食安全和营养安全:未来的挑战
Pub Date : 1900-01-01 DOI: 10.5958/J.2231-1750.3.1.002
Prasanna Kumar
Today humanity is facing a daunting challenge. The natural resources, both on land and water, are increasingly in demand to cater to the human needs and expectations. In order to feed the burgeoning population of India and to attain food security, the mission oriented efforts for increasing food productivity and its sustainability through advanced agro and biotechnological means are to be ventured. However, problems of climate change, biodiversity, desertification, water depletion, pollutants and toxicants, etc are being manifested, which in turn are becoming unfriendly to plant, animal and human health. There is an urgent need for developing methods to alleviate the environmental disorders in order to boost crop productivity. Soil health is defined as the continued capacity of soil to function as a vital living system, by recognising that it contains biological elements that are key to the ecosystem function within land use boundaries. Food security is defined as the state when people have physical and economic access to sufficient, safe and nutritious food to meet their dietary needs and food preferences for an active and healthy life. Future global environment change such as increased climate variability, land degradation and loss of biodiversity, in the context of social, political and economic changes, may bring unprecedented stress on the food production system and food security. Soil health management, however, can play a significant role in climate change mitigation and adaptation to reduce the vulnerability of Indian agriculture. Although worldwide there are clearly enormous number of people to feed, the high yields associated with mono cropping are not worth the long-term environmental and health costs of using fossil fuels and degradation of soil with chemical inputs. Integrated nutrient management, organic farming, biofertilisers, bioremediation, biocontrol and plant growth promoting rhizobacteria are important means for optimising crop production on both short term and long term basis to achieve sustainable production without deterioration of the environment and at the same time maintain the soil fertility.
今天,人类正面临着一项艰巨的挑战。为了满足人类的需要和期望,对土地和水两方面的自然资源的需求日益增加。为了养活印度迅速增长的人口并实现粮食安全,必须冒险进行以任务为导向的努力,通过先进的农业和生物技术手段提高粮食生产力及其可持续性。然而,气候变化、生物多样性、荒漠化、水资源枯竭、污染物和有毒物质等问题正在显现,这些问题反过来又对植物、动物和人类健康变得不友好。为了提高作物产量,迫切需要研究减轻环境失调的方法。土壤健康被定义为土壤作为一个重要的生命系统发挥作用的持续能力,认识到土壤包含对土地利用边界内生态系统功能至关重要的生物要素。粮食安全的定义是,人们在物质上和经济上能够获得充足、安全和有营养的食物,以满足其饮食需求和食物偏好,从而过上积极健康的生活。在社会、政治和经济变化的背景下,未来的全球环境变化,如气候变率增加、土地退化和生物多样性丧失,可能给粮食生产系统和粮食安全带来前所未有的压力。然而,土壤健康管理可以在减缓和适应气候变化方面发挥重要作用,以减少印度农业的脆弱性。虽然世界范围内显然有大量人口需要养活,但单一作物带来的高产量并不值得付出使用化石燃料和化学投入导致土壤退化的长期环境和健康代价。综合养分管理、有机耕作、生物肥料、生物修复、生物防治和促进植物生长的根瘤菌是优化作物短期和长期产量的重要手段,可以在不破坏环境的情况下实现可持续生产,同时保持土壤肥力。
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引用次数: 28
Conventional and Non-Conventional Strategies for Conservation of Medicinal Plants-A Mini Review 药用植物传统与非传统保护策略综述
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2016.00012.3
S. Qadir, B. Ali, Rouf Quadir, A. Fatima, Nahidatun N. Chisti, S. Shah
The medicinal plants have been identified as one of the most important biodiversities and a great treasure bestowed by nature. This biodiversity is constantly being altered throughout the world, both by anthropogenic as well as non-anthropogenic players. Global export of the products obtained from medicinal plants (used both by traditional and modern system of medicine) is exhibiting a declining trend during a couple of decades. The excessive consumption has put a huge pressure on their abundance, sustainability and even threatened the survival of some of the species. Therefore, serious efforts are required at individual, organisational and government level, to protect and conserve medicinal plants and make a judicious use of them. This can be accomplished by adopting different procedures and techniques broadly categorised into (a) conventional method such as in-situ and ex-situ conservation and (b) non-conventional method such as plant tissue culture. This review gives a comprehensive coverage to different ways and means, which can be exploited to conserve the precious wealth of medicinal plants and plant biodiversity in larger perspective.
药用植物已被确定为最重要的生物多样性之一,是大自然赋予的伟大财富。这种生物多样性在世界各地不断受到人为因素和非人为因素的改变。从药用植物(传统和现代医学系统使用)中获得的产品的全球出口在几十年内呈现下降趋势。过度的消费给它们的丰富性和可持续性带来了巨大的压力,甚至威胁到一些物种的生存。因此,需要在个人、组织和政府层面认真努力,保护和保存药用植物,并明智地利用它们。这可以通过采用不同的程序和技术来实现,大致分为(a)传统方法,如原位和非原位保护和(b)非传统方法,如植物组织培养。本文综述了不同的方法和手段,以期从更大的角度保护珍贵的药用植物资源和植物生物多样性。
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引用次数: 1
Germination and Seedling Growth of Coriandrum sativum L. Under Varying Levels of Mixed Cadmium and Copper 不同镉铜混合水平下芫荽种子萌发及幼苗生长研究
Pub Date : 1900-01-01 DOI: 10.5958/J.2231-1742.2.1.008
S. Faizan, Irfana Haneef, S. Kausar, R. Perveen
Elevated levels of heavy metals in contaminated soils are widely spread and concerns have been raised over the potential risks to humans, animals and agricultural crops. This study was conducted to evaluate the effect of mixed cadmium and copper on seed germination and seedling growth of coriander. Treatments were 0 (control), 25, 50, and 100 mg/kg which were made by equal amounts of cadmium (1/2) and copper (1/2). From the data obtained in the presence of different concentration of metals, Germination Index (GI), Metal Tolerance Index (MTI), and Percent Phytotoxicity was calculated. Increasing the concentration of heavy metal mixture to 100 mg/kg showed a significant decrease in seed germination as compared to control treatment (p<0.01). Heavy metal mixture concentrations of 25 mg/kg increased seedling fresh and dry weight (p<0.01) as compared to control. However, the heavy metal mixture concentration of 100 mg/kg produced the lowest amount of both seedling fresh and dry weight. A negative response of root and shoot length of coriander to heavy metal mixture application relative to control treatment was observed at 100 mg/kg (p<0.01). The study suggests that cultivation of coriander in metal polluted soils should be avoided or appropriate control measures be adopted to maintain the heavy metal content of the soil below the damage threshold level.
受污染土壤中重金属含量升高的情况普遍存在,人们对人类、动物和农作物的潜在风险表示担忧。本试验研究了镉铜混合处理对香菜种子萌发和幼苗生长的影响。镉(1/2)和铜(1/2)的处理分别为0(对照)、25、50和100 mg/kg。根据不同浓度金属处理下获得的数据,计算萌发指数(GI)、金属耐受性指数(MTI)和植物毒性百分比。当重金属混合物浓度增加到100 mg/kg时,种子萌发率显著低于对照(p<0.01)。重金属混合物浓度为25 mg/kg时,幼苗鲜重和干重均显著高于对照(p<0.01)。而重金属混合物浓度为100 mg/kg时,对幼苗鲜重和干重的影响最小。施用100 mg/kg重金属时,香菜根、茎长与对照相比呈负相关(p<0.01)。研究表明,应避免在金属污染土壤中种植香菜或采取适当的控制措施,使土壤重金属含量保持在损害阈值以下。
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引用次数: 2
Occurrence of Diseases on Muga Silkworm Host Plant Species Litsea monopetala Roxb. Under Prevailing Weather Conditions during Different Seasons in District Bageshwar, Uttarakhand 家蚕寄主植物Litsea monopetala Roxb的病害发生。在北阿坎德邦Bageshwar地区不同季节的主要天气条件下
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2016.00004.4
Chandrakanta, A. Paliwal, D. Paliwal
Muga silk is cultivated through rearing of the insect silkworm Antheraea assamensis Helfer on the leaves of its host plant Litsea monopetala Roxb. under open weather conditions. During the course of development, the host plants of silkworm are found prone to various diseases. The leaves of host plants because of disease infection become unpalatable for the muga silkworms which ultimately affects on quality and quantity of muga silk production. In the present study, during the year 2012 and 2013, a survey on foliar diseases on the muga silkworm primary host plant L. monopetala Roxb. was carried out in District Bageshwar of Uttarakhand, India and recorded greatest foliar damage (FD) and per cent disease index (PDI) because of the diseases that is red rust (Cephaleuros sp.) FD 19.02% and PDI 11.98, leaf spot (Phyllosticta sp.) FD 7.14% and PDI 5.28, brown blight (Colletotrichum gloeosporioides Penz.) FD 11.63% and PDI 7.31% and grey blight [Pestalotiopsis theae (Thuem.) Stey.] FD 9.30% and PDI 7.11% and others during August-October on the host plant species under open weather during respective years.
蚕丝是通过在寄主植物Litsea monopetala Roxb的叶子上饲养昆虫蚕Antheraea assamensis Helfer来培育的。在开放的天气条件下。蚕的寄主植物在发育过程中容易发生各种疾病。寄主植物的叶片因病害感染而变得难吃,最终影响蚕丝产量和质量。本研究在2012年和2013年对家蚕主要寄主植物L. monopetala Roxb的叶面病害进行了调查。在印度北阿坎德邦的Bageshwar地区进行了叶损(FD)和病害指数(PDI)最大的试验,其中红锈病(Cephaleuros sp.) FD为19.02%,PDI为11.98,叶斑病(Phyllosticta sp.) FD为7.14%,PDI为5.28,褐枯病(Colletotrichum gloeosporioides Penz.)FD 11.63%, PDI 7.31%;灰疫病[拟盘多毛孢];Stey。8 - 10月对不同年份露天条件下寄主植物的FD值为9.30%,PDI值为7.11%等。
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引用次数: 0
Screening of Withania somnifera L. Germplasm for Resistance against Leaf Spot Caused by Alternaria alternata (Fr.) Keissler 稻瘟病抗性稻瘟病种质的筛选
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2016.00009.3
Mohammad Zuhaib, Shabbir Ashraf
Ashwagandha (Withania somnifera L. Dunal), commonly called as ‘winter cherry. The roots, leaves and fruits of this medicinal plant possess tremendous medicinal value. In recent years the demand of Ashwagandha in the international market increases by many folds. Plant diseases are the major constraints in the production of Ashwagandha. In this study seven Ashwagandha genotypes were screened against Alternaria leaf spot under pot condition on the basis of disease severity and percent reduction in yield. Among seven genotypes RAS-10 Showed consistently resistant reaction. IC-310620(A) and IC-283942 were tolerant and moderately tolerant, while remaining genotypes were found to be susceptible to highly susceptible. During the estimation of reduction in chlorophyll and carotenoid content of the 7 genotypes, the reduction in chlorophyll content was maximum in genotype RAS-10 and minimum in IC-283942. The reduction in carotenoid content was maximum in genotype RAS-98 and minimum in RAS-7.
Ashwagandha (Withania somnifera L. Dunal),通常被称为“冬季樱桃”。这种药用植物的根、叶和果实具有巨大的药用价值。近年来,国际市场对阿什瓦甘达的需求增长了许多倍。植物病害是制约阿什瓦甘达生产的主要因素。本研究在盆栽条件下,根据病害严重程度和减产率筛选了7个印度甘薯基因型。在7个基因型中,RAS-10表现出一致的耐药反应。IC-310620(A)和IC-283942为耐和中耐型,其余基因型为高敏感型。在对7个基因型叶绿素和类胡萝卜素含量降低的估计中,基因型RAS-10的叶绿素含量降低幅度最大,IC-283942的叶绿素含量降低幅度最小。类胡萝卜素含量的降低在基因型RAS-98中最大,在基因型RAS-7中最小。
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引用次数: 2
Effect of Different Concentration of Thiamine Hydrochloride (Vitamin-B1) on Growth and Yield of Mustard (Brassica juncea L.) var. Varuna 不同浓度盐酸硫胺素(维生素b1)对芥菜生长和产量的影响
Pub Date : 1900-01-01 DOI: 10.5958/J.2231-1750.3.2.014
A. Sajjad, Samiullah
One pot experiment was conducted according to simple randomised block design on Mustard (Brassica juncea L.) Var. Varuna to establish the most suitable concentration for soaking of mustard seeds in thiamine hydrochloride solutions of concentrations, that is, 0.01, 0.02, 0.03, 0.06, 0.09, 0.12% on the performance of mustard. The seeds were soaked in thiamine hydrochloride solutions for 4 h. Water-soaked seeds were treated as control. Uniform basal dose of N90, P30 and K30 fertilizers were given to the soil before filling in the pots at the rate of NPKkg/ha. The soaked seeds were sown in clay pots containing autoclaved sandy loam soil and compost in 3:1 mixture. The parameters such as shoot dry weight, root dry weight, leaf area, crop growth rate (CGR), relative growth rate (RGR) and net assimilation rate (NAR) were calculated from 40 to 120 days after sowing. Number of pods per plant, 1,000 seed weight, yield, biological yield and harvest index were measured at harvest. Soaking concentration of 0.03% of thiamine hydrochloride was found to be the best in comparison to any other concentration of the treatment for most of the parameter studied. In growth parameters, leaf area, plant dry weight, root dry weight showed significant results of the early stages of sampling [that is, 40–80 Days after sowing (DAS)]. CGR also followed the same trend, however, RGR and NAR did not follow the same at any stage of the sampling. Yield parameters like pod number per plant, biological yield and seed yield registered significant result. Thus, it was concluded that soaking of mustard seeds in 0.03% thiamine hydrochloride ensured better growth and yield.
采用简单随机区组设计对芥菜(Brassica juncea L.)进行盆栽试验。以Varuna . Varuna为研究对象,确定了在盐酸硫胺素溶液中浸泡芥菜籽的最适宜浓度,即0.01、0.02、0.03、0.06、0.09、0.12%对芥菜性能的影响。将种子在盐酸硫胺素溶液中浸泡4 h,水浸种子作为对照。填盆前均匀施硝态氮(N90)、硝态氮(P30)和硝态氮(K30)基肥,施量为NPKkg/ha。将浸透的种子播种在装有蒸压砂壤土和堆肥的粘土罐中,混合比例为3:1。在播种后40 ~ 120 d,计算地上部干重、根干重、叶面积、作物生长率(CGR)、相对生长率(RGR)和净同化率(NAR)等参数。收获时测定单株荚果数、千粒重、产量、生物产量和收获指数。对于大多数研究的参数,与其他任何浓度的处理相比,发现0.03%盐酸硫胺素的浸泡浓度是最好的。在生长参数方面,叶面积、植株干重、根系干重在取样初期(即播种后40-80天)表现出显著的结果。CGR也遵循相同的趋势,然而,RGR和NAR在采样的任何阶段都没有遵循相同的趋势。单株荚果数、生物产量和种子产量等产量参数均取得显著成果。由此可见,在0.03%盐酸硫胺素中浸泡芥菜籽能获得较好的生长和产量。
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引用次数: 0
Response of Melissa officinalis Induced by Application of Pseudomonas fluorescens Isolated from Different Agro Climatic Zones of Karnataka 卡纳塔克邦不同农业气候带荧光假单胞菌对茉莉的诱导作用
Pub Date : 1900-01-01 DOI: 10.5958/2231-1750.2015.00001.3
S. Bohra, N. Krithika, M. Nanditha, G. Sudha, C. Sandeep, C. K. Suresh
Melissa officinalis is an aromatically important plant grown all over the world. It is used for different medical purposes as tonic, antispasmodic, carminative, diaphoretic, sedative-hypnotic strengthening the memory, relief of stress-induced headache and has antioxidant properties. The fluorescent Pseudomonas have emerged as the largest and potentially most promising group of the plant growth promoting rhizobacteria with their simple nutritional requirement, flexible metabolism, their short generation time and motility. Through several mechanisms, they promote plant growth and inhibit soil borne or seed borne phytopathogens. This present work is carried out in Department of Biotechnology, U.A.S., G.K.V.K., Bangalore. This study aims to isolate and identify the Psuedomonas fluorescens from rhizospheric soil from different agroclimatic zones of Karnataka. Molecular characterisation was done through the RAPD technique. The isolates found to be highly polymorphic. The RAPD bands are analysed by Statistica software. The dendrogram clearly depicted that all the isolates form two major clusters and isolates from zone 1 and zone 4 spanning the extremes. Efficient strain of Pseudomanas was screened out, which is responsible for increasing biomass of Melissa officinalis. Ten treatments of Pseudomonas fluorescens are made from ten different agro climatic zones with one control. These treatments are inoculated on 2 week-old seedlings of Melissa officinalis and allowed to grow for 60 days. Plant height, weight, chlorophyll content, total soluble sugars, and proteins are estimated at a regular interval of time. All the data are subjected to MStat C software for statistical analysis. It is observed that the Psuedomonas fluorescen isolated from zone 6 induced potentially increased growth in Melissa officinalis and is responsible for plant growth promotion.
梅莉莎(Melissa officinalis)是一种重要的芳香植物,生长在世界各地。它被用于不同的医疗目的,如滋补,抗痉挛,驱风,发汗,镇静催眠,加强记忆,缓解压力引起的头痛,并具有抗氧化特性。荧光假单胞菌以其营养需求简单、代谢灵活、生成时间短、能动性强等优点,成为植物促生菌中最大的一类,也是最有发展潜力的一类。它们通过多种机制促进植物生长,抑制土壤或种子传播的植物病原体。目前的工作是在美国班加罗尔g.k.v.k.生物技术部门进行的。本研究旨在从卡纳塔克邦不同农业气候带的根际土壤中分离和鉴定荧光假单胞菌。通过RAPD技术进行分子表征。发现分离株具有高度多态性。利用Statistica软件对RAPD波段进行分析。树状图清楚地描述了所有分离株形成两个主要集群,并且分离株来自1区和4区,跨越了两个极端。筛选出了对茉莉生物量增加有重要作用的假单胞菌高效菌株。荧光假单胞菌的十种处理方法是在一个对照下从十个不同的农业气候带制成的。这些处理接种在2周龄的梅莉莎幼苗上,让其生长60天。植物的高度、重量、叶绿素含量、总可溶性糖和蛋白质按一定的时间间隔进行估计。所有数据交由MStat C软件进行统计分析。结果表明,从6区分离的荧光假单胞菌对茉莉的生长具有潜在的促进作用,具有促进植株生长的作用。
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引用次数: 0
Application of Methanolic Extracts of Botanicals for the Management of M. incognita Infecting Tomato and Chilli 植物甲醇提取物在番茄、辣椒黑僵菌防治中的应用
Pub Date : 1900-01-01 DOI: 10.5958/J.2231-1742.1.2.012
A. Usman, M. Siddiqui
Leaf extracts of two plants such as Murraya koenigii L. Spreng and Vitex negundo L. were used as bare-root dip treatment for the management of phytonematode, Meloidogyne incognita infecting tomato (Lycopersicon esculentum) and chilli (Capsicum annum) plants. Significant reduction was observed in the root-knot development caused by M. incognita on the experimental plants. Leaf extracts of Murraya caused relatively higher inhibition in root-knot development and nematode multiplication than Vitex. Improvement in plant growth was noted. The efficacy of root-dip treatment with respect to improvement in plant weight and reduction in disease incidence increased with the increase in concentration of leaf extracts and dip duration.
以两种植物的叶片提取物(Murraya koenigii L.春)和牡荆(Vitex negundo L.)为原料,进行裸根浸染处理,防治番茄(Lycopersicon esculentum)和辣椒(Capsicum annum)的植物线虫(Meloidogyne incognita)。结果表明,黑穗病菌对试验植株的根结发育有明显的抑制作用。枸杞叶提取物对根结发育和线虫繁殖的抑制作用高于牡荆。注意到植物生长的改善。根浸处理在改善植株重量和降低病害发生率方面的效果随着叶片提取物浓度的增加和浸渍时间的延长而增加。
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引用次数: 3
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Journal of Functional and Environmental Botany
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