Shasha Zhang, Leiyan Su, Zefeng Kang, Shoukang Zhang, Jing Wang
Purpose: To investigate the clinical efficacy of pranoprofen eye drops when combined with emedastinedifumarate eye drops for the treatment of children with allergic conjunctivitis.Methods: A total of 96 children with allergic conjunctivitis admitted to Eye Hospital, China Academy of Chinese Medical Sciences from January 2020 to 2022 were enrolled in this study. They were divided into study group (treated with both pranoprofen eye drops and emedastine difumarate eye drops), and control group (treated with emedastine difumarate eye drops alone), with 48 children in each group. The control group was given emedastine fumarate eye drops 1 drop twice a day. The study group was given pranoprofen eye drops 2 drops 4 times a day in addition to the treatment administered to control group. Patients in both groups were observed and compared after one week of treatment. Efficacy, incidence of adverse reactions, symptom scores, inflammatory factors, tear film stability indicators and immune function indicators were assessed and recorded.Results: Prior to treatment, there was no significant difference in symptom score, inflammatory factors, tear film stability indicators and immune function indicators between study and control groups. Although the above indicators improved in both groups after treatment, the study group showed significantly greater improvement than control group (p < 0.05). Overall clinical response rate in the study group (95.83 %) was higher than in the control group (77.08 %), while the incidence of adverse reaction in the study group (6.25 %) was lower than that of control group (27.08 %, p < 0.05).Conclusion: Pranoprofen eye drops, when combined with emedastine difumarate eye drops for treatment of children with allergic conjunctivitis, is more effective than the use of emedastine difumarate eye drops alone. However, further clinical trials are required to validate the findings of this study prior to adoption of the combination treatment in clinical practice.
{"title":"Effect of co-administration of pranoprofen/emedastine difumarate eye drops on efficacy, inflammatory factors, tear film stability and immune function in children with allergic conjunctivitis","authors":"Shasha Zhang, Leiyan Su, Zefeng Kang, Shoukang Zhang, Jing Wang","doi":"10.4314/tjpr.v22i7.20","DOIUrl":"https://doi.org/10.4314/tjpr.v22i7.20","url":null,"abstract":"Purpose: To investigate the clinical efficacy of pranoprofen eye drops when combined with emedastinedifumarate eye drops for the treatment of children with allergic conjunctivitis.Methods: A total of 96 children with allergic conjunctivitis admitted to Eye Hospital, China Academy of Chinese Medical Sciences from January 2020 to 2022 were enrolled in this study. They were divided into study group (treated with both pranoprofen eye drops and emedastine difumarate eye drops), and control group (treated with emedastine difumarate eye drops alone), with 48 children in each group. The control group was given emedastine fumarate eye drops 1 drop twice a day. The study group was given pranoprofen eye drops 2 drops 4 times a day in addition to the treatment administered to control group. Patients in both groups were observed and compared after one week of treatment. Efficacy, incidence of adverse reactions, symptom scores, inflammatory factors, tear film stability indicators and immune function indicators were assessed and recorded.Results: Prior to treatment, there was no significant difference in symptom score, inflammatory factors, tear film stability indicators and immune function indicators between study and control groups. Although the above indicators improved in both groups after treatment, the study group showed significantly greater improvement than control group (p < 0.05). Overall clinical response rate in the study group (95.83 %) was higher than in the control group (77.08 %), while the incidence of adverse reaction in the study group (6.25 %) was lower than that of control group (27.08 %, p < 0.05).Conclusion: Pranoprofen eye drops, when combined with emedastine difumarate eye drops for treatment of children with allergic conjunctivitis, is more effective than the use of emedastine difumarate eye drops alone. However, further clinical trials are required to validate the findings of this study prior to adoption of the combination treatment in clinical practice.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"76 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86782152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junxin Qiu, Juan Zhao, Ping Feng, Huiqin Zhou, Haiyang Li
Purpose: To investigate the correlation between traditional Chinese medicine (TCM) constitution and pregnancy outcomes in elderly primiparae.Methods: A cross-sectional case-control study was conducted on 113 elderly primiparae who were delivered in Jinshan Hospital of Fudan University, Shanghai, China from January 2019 to December 2019. The elderly primiparae was the study group. Using a random ratio of 1:3, 393 primiparous women of suitable age who were delivered in the hospital at the same period were selected as the control group. A traditional Chinese medicine (TCM) questionnaire was used to collect clinical data, and differences between the two groups were compared.Results: The incidence of gestational diabetes mellitus was significantly higher in the study group (elderl y primiparae) with phlegm dampness constitution and qi stagnation constitution than in control group (appropriate-age primiparae) (p < 0.05). The incidence of hypertension was significantly higher in the study group with yin deficiency constitution and special constitution than in control group (p < 0.05). The frequency of selective cesarean section in study group with a biased qi-deficiency constitution, yang-deficiency constitution, and yin-deficiency constitution were significantly higher than control group (p < 0.05).Conclusion: There is a correlation between pregnancy outcomes in old age and TCM constitution. Individualized diet and exercise programs may be formulated in early pregnancy (based on the type of physique) to improve pregnancy outcomes in elderly primiparae.
{"title":"Relationship between traditional Chinese medicine constitution and pregnancy outcome in elderly primiparae","authors":"Junxin Qiu, Juan Zhao, Ping Feng, Huiqin Zhou, Haiyang Li","doi":"10.4314/tjpr.v22i7.12","DOIUrl":"https://doi.org/10.4314/tjpr.v22i7.12","url":null,"abstract":"Purpose: To investigate the correlation between traditional Chinese medicine (TCM) constitution and pregnancy outcomes in elderly primiparae.Methods: A cross-sectional case-control study was conducted on 113 elderly primiparae who were delivered in Jinshan Hospital of Fudan University, Shanghai, China from January 2019 to December 2019. The elderly primiparae was the study group. Using a random ratio of 1:3, 393 primiparous women of suitable age who were delivered in the hospital at the same period were selected as the control group. A traditional Chinese medicine (TCM) questionnaire was used to collect clinical data, and differences between the two groups were compared.Results: The incidence of gestational diabetes mellitus was significantly higher in the study group (elderl y primiparae) with phlegm dampness constitution and qi stagnation constitution than in control group (appropriate-age primiparae) (p < 0.05). The incidence of hypertension was significantly higher in the study group with yin deficiency constitution and special constitution than in control group (p < 0.05). The frequency of selective cesarean section in study group with a biased qi-deficiency constitution, yang-deficiency constitution, and yin-deficiency constitution were significantly higher than control group (p < 0.05).Conclusion: There is a correlation between pregnancy outcomes in old age and TCM constitution. Individualized diet and exercise programs may be formulated in early pregnancy (based on the type of physique) to improve pregnancy outcomes in elderly primiparae.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"42 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84982990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tri Truong Van, Nhi Ho Linh Kieu, Huyen Nguyen Thi Thuong
Purpose: To investigate the effect of varying zinc concentrations on weight gain, hematological parameters, and tissue structure of the liver, kidney, and spleen in albino mice.Methods: The mice were administered daily zinc at 50, 70, and 90 mg/kg body weight. Weight gain was measured every two weeks. Hematological parameters were evaluated after 4 and 8 weeks, while biomolecular parameters were assessed after 8 weeks. Liver, kidney, and spleen were also assessed after 8 weeks using H & E staining procedures.Results: Albino mice exhibited reduced weight gain, and altered hematological parameters after 8 weeks of exposure to varying zinc concentrations, with the highest dose of 90 mg/kg body weight having the most significant impact. Hematological parameters including blood cells, hemoglobin, hematocrit, blood clotting time, aspartate aminotransferase, and alanine transaminase decreased, while bilirubin, urea, and mean corpuscular hemoglobin concentration increased. Liver and kidney tissues exhibited widespread destruction such as necrosis and hemorrhage, while red pulp invaded white pulp activity in the spleen.Conclusion: High levels of zinc from zinc chloride (90 mg/kg) affect body weight, and hematological parameters, as well as liver and kidney histology in albino mice. The most significant negative effects include the potential toxicity and adverse health consequences of excessive zinc intake. Consequently, this study provides the groundwork for investigating natural compounds that inhibit zinc toxicity.
{"title":"Effect of zinc on weight gain, hematological parameters and tissue structure of the liver, kidney and spleen in albino mice","authors":"Tri Truong Van, Nhi Ho Linh Kieu, Huyen Nguyen Thi Thuong","doi":"10.4314/tjpr.v22i7.7","DOIUrl":"https://doi.org/10.4314/tjpr.v22i7.7","url":null,"abstract":"Purpose: To investigate the effect of varying zinc concentrations on weight gain, hematological parameters, and tissue structure of the liver, kidney, and spleen in albino mice.Methods: The mice were administered daily zinc at 50, 70, and 90 mg/kg body weight. Weight gain was measured every two weeks. Hematological parameters were evaluated after 4 and 8 weeks, while biomolecular parameters were assessed after 8 weeks. Liver, kidney, and spleen were also assessed after 8 weeks using H & E staining procedures.Results: Albino mice exhibited reduced weight gain, and altered hematological parameters after 8 weeks of exposure to varying zinc concentrations, with the highest dose of 90 mg/kg body weight having the most significant impact. Hematological parameters including blood cells, hemoglobin, hematocrit, blood clotting time, aspartate aminotransferase, and alanine transaminase decreased, while bilirubin, urea, and mean corpuscular hemoglobin concentration increased. Liver and kidney tissues exhibited widespread destruction such as necrosis and hemorrhage, while red pulp invaded white pulp activity in the spleen.Conclusion: High levels of zinc from zinc chloride (90 mg/kg) affect body weight, and hematological parameters, as well as liver and kidney histology in albino mice. The most significant negative effects include the potential toxicity and adverse health consequences of excessive zinc intake. Consequently, this study provides the groundwork for investigating natural compounds that inhibit zinc toxicity.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"25 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82014930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Immune checkpoint inhibitors (ICIs) have been widely used in non-small cell lung cancer with wildtype EGFR/ALK genes. However, the effect of ICIs on advanced NSCLC with EGFR genes sensitive mutations has been controversial. There are no reports on whether ICIs monotherapy can be used in the treatment of NSCLC with EGFR sensitive mutations. A patient with metastatic lung squamous cell carcinoma with EGFR G719s mutations, high PD-L1 score and a tuberculosis history was given first-line treatment of afatinib, camrelizumab monotherapy (200 mg, intravenous, once every three weeks) from February 24,2020 to January 2023. The patient achieved partial response (PR) after treatment with camrelizumab. The progress-free survival (PFS) due to camrelizumab monotherapy was more than 34 months.It is concluded that camrelizumab has promising potential effectiveness as a treatment option for lung squamous cell cancer with EGFR G719 mutations and high PD-L1 score.
{"title":"Long response to camrelizumab in metastatic lung squamous cell carcinoma with high PD-L1 expression despite EGFR G719S mutation: A case report","authors":"Yuhua Wang, Ke Fang","doi":"10.4314/tjpr.v22i7.24","DOIUrl":"https://doi.org/10.4314/tjpr.v22i7.24","url":null,"abstract":"Immune checkpoint inhibitors (ICIs) have been widely used in non-small cell lung cancer with wildtype EGFR/ALK genes. However, the effect of ICIs on advanced NSCLC with EGFR genes sensitive mutations has been controversial. There are no reports on whether ICIs monotherapy can be used in the treatment of NSCLC with EGFR sensitive mutations. A patient with metastatic lung squamous cell carcinoma with EGFR G719s mutations, high PD-L1 score and a tuberculosis history was given first-line treatment of afatinib, camrelizumab monotherapy (200 mg, intravenous, once every three weeks) from February 24,2020 to January 2023. The patient achieved partial response (PR) after treatment with camrelizumab. The progress-free survival (PFS) due to camrelizumab monotherapy was more than 34 months.It is concluded that camrelizumab has promising potential effectiveness as a treatment option for lung squamous cell cancer with EGFR G719 mutations and high PD-L1 score. ","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"194 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74428293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To systematically review the efficacy of paclitaxel-carboplatin versus trastuzumab in combination with paclitaxel/carboplatin for the treatment of uterine serous carcinoma (USC) with high Her2/neu expression, in order to obtain an evidence-based reference for clinical treatment.Methods: Publications were obtained from China Knowledge Network (CNKI), VIP database (VIP), Wanfang database, PubMed, Embase, Cochrane Library, and Web of Science. The retrieval period was from the establishment of database to May 2022, and the efficacy of paclitaxel-carboplatin combination with trastuzumab (study group) versus paclitaxel-carboplatin (control group) for the treatment of Her2/neu high-expressing USC was collected. Two reviewers independently screened, extracted data, and evaluated the quality of evidence of included studies. RevMan 5.3 statistical software was used for meta-analysis. Outcomes included overall survival (OS), progression-free survival (PFS), objective response rate (ORR), and adverse events.Results: Meta-analysis showed that the study group had significantly longer OS than control group (p = 0.004), and PFS of study group was significantly longer than that of control group (p < 0.0001). In terms of ORR, there was no difference between the two groups (p = 0.42). However, in terms of total and high-risk adverse events, the number of patients with adverse events in study group was higher than in control group (p = 0.05), (p = 0.0001).Conclusion: Paclitaxel-carboplatin combination with trastuzumab significantly prolongs OS and PFS in USC patients with high Her2/neu expression, but it may increase the incidence of adverse events.
{"title":"Effect of the combination of trastuzumab, paclitaxel and carboplatin in the treatment of uterine serous carcinoma: A systematic review and meta-analysis","authors":"Xiaochuan Yu, Siyu Ma, Huali Wang","doi":"10.4314/tjpr.v22i7.23","DOIUrl":"https://doi.org/10.4314/tjpr.v22i7.23","url":null,"abstract":"Purpose: To systematically review the efficacy of paclitaxel-carboplatin versus trastuzumab in combination with paclitaxel/carboplatin for the treatment of uterine serous carcinoma (USC) with high Her2/neu expression, in order to obtain an evidence-based reference for clinical treatment.Methods: Publications were obtained from China Knowledge Network (CNKI), VIP database (VIP), Wanfang database, PubMed, Embase, Cochrane Library, and Web of Science. The retrieval period was from the establishment of database to May 2022, and the efficacy of paclitaxel-carboplatin combination with trastuzumab (study group) versus paclitaxel-carboplatin (control group) for the treatment of Her2/neu high-expressing USC was collected. Two reviewers independently screened, extracted data, and evaluated the quality of evidence of included studies. RevMan 5.3 statistical software was used for meta-analysis. Outcomes included overall survival (OS), progression-free survival (PFS), objective response rate (ORR), and adverse events.Results: Meta-analysis showed that the study group had significantly longer OS than control group (p = 0.004), and PFS of study group was significantly longer than that of control group (p < 0.0001). In terms of ORR, there was no difference between the two groups (p = 0.42). However, in terms of total and high-risk adverse events, the number of patients with adverse events in study group was higher than in control group (p = 0.05), (p = 0.0001).Conclusion: Paclitaxel-carboplatin combination with trastuzumab significantly prolongs OS and PFS in USC patients with high Her2/neu expression, but it may increase the incidence of adverse events.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"12 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87982175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To investigate the influence of the combined use of platelet-rich fibrin and narrow-band UV B (NB-UVB) treatment on small-area deep burn wounds in patients.Methods: A total of 86 patients with small area deep burn wounds were assigned to control and study groups (n = 43/group). The control patients received routine drugs in combination with NB-UVB, while the study group was administered a combination of platelet-rich fibrin and NB-UVB therapy. Clinical efficacy indices were.Results: In the study group, the pre and post-treatment serum levels of TNF-α were 222.75 ± 4.86 and 65.42 ± 5.33 ng/L, while those in control group were 221.45 ± 6.84 and 114.68 ± 2.53 ng/L, respectively. The study group serum concentrations of IL-8 at pre- and post-treatment were 120.75 ± 4.53 ng/L and 45.39 ± 8.26 ng/L, while the control group values were 122.38 ± 2.65 and 79.52 ± 2.34 ng/L, respectively. After treatment, the serum levels of TNF-α and IL-8 were significantly down-regulated in both groups, relative to pre-treatment values, with lower levels in the study group (p < 0.001). Wound healing time and frequency of dressing change in the study group were 20.6 ± 3.6 days and 7.1 ± 2.5 times, respectively, which were significantly lower than the corresponding control group values of 40.3 ± 10.7 days and 20.5 ± 5.6 times (p < 0.001). There was a significantly higher incidence of scars in the control group than in study group (p < 0.05).Conclusion: Combined use of platelet-rich fibrin and NB-UVB in the treatment of small area deep burn wounds lowers the serum levels of inflammatory factors. However, the combined therapy should be subjected to further clinical trials prior to application in clinical practice.
{"title":"Clinical effect of platelet-rich fibrin in combination with narrow-band ultraviolet B treatment in patients with small area deep burn wounds","authors":"Jialei Yang, Xia Li, H. Liu, Xiao Zhao","doi":"10.4314/tjpr.v22i7.13","DOIUrl":"https://doi.org/10.4314/tjpr.v22i7.13","url":null,"abstract":"Purpose: To investigate the influence of the combined use of platelet-rich fibrin and narrow-band UV B (NB-UVB) treatment on small-area deep burn wounds in patients.Methods: A total of 86 patients with small area deep burn wounds were assigned to control and study groups (n = 43/group). The control patients received routine drugs in combination with NB-UVB, while the study group was administered a combination of platelet-rich fibrin and NB-UVB therapy. Clinical efficacy indices were.Results: In the study group, the pre and post-treatment serum levels of TNF-α were 222.75 ± 4.86 and 65.42 ± 5.33 ng/L, while those in control group were 221.45 ± 6.84 and 114.68 ± 2.53 ng/L, respectively. The study group serum concentrations of IL-8 at pre- and post-treatment were 120.75 ± 4.53 ng/L and 45.39 ± 8.26 ng/L, while the control group values were 122.38 ± 2.65 and 79.52 ± 2.34 ng/L, respectively. After treatment, the serum levels of TNF-α and IL-8 were significantly down-regulated in both groups, relative to pre-treatment values, with lower levels in the study group (p < 0.001). Wound healing time and frequency of dressing change in the study group were 20.6 ± 3.6 days and 7.1 ± 2.5 times, respectively, which were significantly lower than the corresponding control group values of 40.3 ± 10.7 days and 20.5 ± 5.6 times (p < 0.001). There was a significantly higher incidence of scars in the control group than in study group (p < 0.05).Conclusion: Combined use of platelet-rich fibrin and NB-UVB in the treatment of small area deep burn wounds lowers the serum levels of inflammatory factors. However, the combined therapy should be subjected to further clinical trials prior to application in clinical practice.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"96 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87220961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhan Yao, Shuna Liu, Chunmei Zheng, Qiangwu Li, Liya Wang
Purpose: To determine the effect of procyanidin A1 (PCA1) on sepsis.Methods: Dulbecco’s Modified Eagle Medium (DMEM) was employed to incubate mouse hepatic cell line AML12. The AML12 cells treated with lipopolysaccharide (LPS, 50 μg/mL) was used to establish a sepsis cell model. Cell viability was evaluated using CCK-8 assay, while cell apoptosis was assessed by flow cytometry. Aspartate transaminase (AST), alanine aminotransferase (ALT), IL-6 and TNF-α levels were evaluated by enzyme linked immunosorbent assay (ELISA). Protein expressions were assessed using western blot assay.Results: The viability of AML12 cells decreased following treatment with IL-1β, but this change was offset by PCA1 treatment (40 or 80 μM). Similarly, cell apoptosis was enhanced after LPS treatment, but this change was attenuated by PCA1 treatment. The AST, ALT, IL-6 and TNF-α levels were all elevated after LPS treatment, but these changes were also reversed by PCA1 treatment, indicating that PCA1 suppressed LPS-induced liver injury and inflammation. Furthermore, the protein levels of p-p65/p65 and p-IκBα increased, and IκBα lowered following LPS treatment, but these effects were reversed by PCA1 treatment, indicating that PCA1 retarded NF-κB pathway.Conclusion: PCA1 alleviates sepsis-induced liver injury by inhibiting inflammation through NF-κB pathway. This suggestes that PCA1 may be an therapeutic agent for the treatment of sepsis.
{"title":"Procyanidin A1 improves sepsis-induced liver injury by inhibiting inflammation","authors":"Zhan Yao, Shuna Liu, Chunmei Zheng, Qiangwu Li, Liya Wang","doi":"10.4314/tjpr.v22i7.8","DOIUrl":"https://doi.org/10.4314/tjpr.v22i7.8","url":null,"abstract":"Purpose: To determine the effect of procyanidin A1 (PCA1) on sepsis.Methods: Dulbecco’s Modified Eagle Medium (DMEM) was employed to incubate mouse hepatic cell line AML12. The AML12 cells treated with lipopolysaccharide (LPS, 50 μg/mL) was used to establish a sepsis cell model. Cell viability was evaluated using CCK-8 assay, while cell apoptosis was assessed by flow cytometry. Aspartate transaminase (AST), alanine aminotransferase (ALT), IL-6 and TNF-α levels were evaluated by enzyme linked immunosorbent assay (ELISA). Protein expressions were assessed using western blot assay.Results: The viability of AML12 cells decreased following treatment with IL-1β, but this change was offset by PCA1 treatment (40 or 80 μM). Similarly, cell apoptosis was enhanced after LPS treatment, but this change was attenuated by PCA1 treatment. The AST, ALT, IL-6 and TNF-α levels were all elevated after LPS treatment, but these changes were also reversed by PCA1 treatment, indicating that PCA1 suppressed LPS-induced liver injury and inflammation. Furthermore, the protein levels of p-p65/p65 and p-IκBα increased, and IκBα lowered following LPS treatment, but these effects were reversed by PCA1 treatment, indicating that PCA1 retarded NF-κB pathway.Conclusion: PCA1 alleviates sepsis-induced liver injury by inhibiting inflammation through NF-κB pathway. This suggestes that PCA1 may be an therapeutic agent for the treatment of sepsis.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"37 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78051297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lan Shou, Lingling Zhou, Jinhua Hu, Qianru Zhu, Hong Luo
Purpose: To investigate the potential role of sanggenon C alleviating in insulin resistance.Methods: HepG2 cell line was incubated with increasing concentrations of sanggenon C at 1, 5, 10, 15 or 20 μM for 4 h. to induce cytotoxicity, and then further incubated with 100 μM palmitic acid to induce insulin resistance. HepG2 cells without sanggenon C and palmitic acid treatment servered as control group. Glucose uptake was determined by measuring 2-NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)-amino]-D-glucose) fluorescence intensity using a microplate reader. Oil Red O staining was used to assess intracellular lipid accumulation, while oxidative stress was evaluated by enzyme-linked immunosorbent assay (ELISA).Results: Palmitic acid significantly decreased glucose uptake and increased intracellular lipid accumulation in HepG2 (p < 0.01), while sanggenon C enhanced t glucose uptake and lowered lipid accumulation in insulin-resistant HepG2 (p < 0.01). Sanggenon C significantly attenuated palmitic acidinduced increase in p-insulin receptor substrate 1 (p-IRS1), as well as decrease in p-AKT and p-FOXO1 (p < 0.01). Palmitic acid also induced oxidative stress in HepG2 through the up-regulation of reactive oxygen species (ROS) and malondialdehyde (MDA), as well as the down-regulation of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). However, sanggenon C reduced ROS and MDA levels (p < 0.05), and enhanced SOD and GSH-Px in insulin-resistant HepG2 (p < 0.05). However, sanggenon C significantly increased p-AMP-activated protein kinase (p-AMPK) levels and p-ACC (acetyl-CoA carboxylase) in insulin-resistant HepG2 (p < 0.01).Conclusion: Sanggenon C lowers oxidative stress and ameliorates lipid accumulation thereby alleviating palmitic acid-induced insulin-resistant HepG2 cells via activation of AMPK pathway, thus suggesting that it is a potential strategy for overcoming insulin resistance.
{"title":"Sanggenon C alleviates palmitic acid-induced insulin resistance in HepG2 cells via AMPK pathway","authors":"Lan Shou, Lingling Zhou, Jinhua Hu, Qianru Zhu, Hong Luo","doi":"10.4314/tjpr.v22i7.1","DOIUrl":"https://doi.org/10.4314/tjpr.v22i7.1","url":null,"abstract":"Purpose: To investigate the potential role of sanggenon C alleviating in insulin resistance.Methods: HepG2 cell line was incubated with increasing concentrations of sanggenon C at 1, 5, 10, 15 or 20 μM for 4 h. to induce cytotoxicity, and then further incubated with 100 μM palmitic acid to induce insulin resistance. HepG2 cells without sanggenon C and palmitic acid treatment servered as control group. Glucose uptake was determined by measuring 2-NBDG (2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)-amino]-D-glucose) fluorescence intensity using a microplate reader. Oil Red O staining was used to assess intracellular lipid accumulation, while oxidative stress was evaluated by enzyme-linked immunosorbent assay (ELISA).Results: Palmitic acid significantly decreased glucose uptake and increased intracellular lipid accumulation in HepG2 (p < 0.01), while sanggenon C enhanced t glucose uptake and lowered lipid accumulation in insulin-resistant HepG2 (p < 0.01). Sanggenon C significantly attenuated palmitic acidinduced increase in p-insulin receptor substrate 1 (p-IRS1), as well as decrease in p-AKT and p-FOXO1 (p < 0.01). Palmitic acid also induced oxidative stress in HepG2 through the up-regulation of reactive oxygen species (ROS) and malondialdehyde (MDA), as well as the down-regulation of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). However, sanggenon C reduced ROS and MDA levels (p < 0.05), and enhanced SOD and GSH-Px in insulin-resistant HepG2 (p < 0.05). However, sanggenon C significantly increased p-AMP-activated protein kinase (p-AMPK) levels and p-ACC (acetyl-CoA carboxylase) in insulin-resistant HepG2 (p < 0.01).Conclusion: Sanggenon C lowers oxidative stress and ameliorates lipid accumulation thereby alleviating palmitic acid-induced insulin-resistant HepG2 cells via activation of AMPK pathway, thus suggesting that it is a potential strategy for overcoming insulin resistance. ","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"2014 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88167926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: To investigate the therapeutic effect of combined administration of laser and Fufang xueshuantong capsules in patients with diabetic retinopathy, and its influence on ocular hemodynamics.Methods: A total of 193 patients with diabetic retinopathy in the Ophthalmology Department of Hebei University Affiliated Hospital from October 2020 to April 2022 were retrospectively analyzed. The patients were divided into two groups: study group (127 patients) and control group (66 patients). Control group received laser therapy in the fundus while the study group was administered 3 capsules (i.e., 1.5 g) of Fufang xueshuantong thrice daily for 7 days. Changes in the fundus and visual acuity, hemodynamic indices of the ophthalmic artery, and changes in macular edema diameter, and adverse reactions in both groups were recorded.Results: In the study group, after treatment, eyeground changes were especially effective (67.37 %), effective (30.51 %), or ineffective (2.12 %). Visual acuity improved (58.90 %), (unchanged at 38.56 %), or decreased (2.54 %). In the control group, laser treatment was especially effective (42.61 %), effective (44.35 %), or ineffective (13.04 %); however, visual acuity increased (36.52 %), unchanged (47.83 %), or decreased (15.65 %). The changes were significant (p < 0.05). There was no significant difference in ophthalmic artery hemodynamics between the two groups before treatment (p > 0.05). After treatment, however, peak systolic velocity (Psv), end diastolic blood flow velocity (Edv), mean velocity (Vm), resistance index (RI), and pulsatile index (PI) in the study group were significantly different from those of the control group (p < 0.05); There was also no significant difference between the two groups before and 1 day after the operation (p > 0.05), neither was there a significant difference in adverse reactions between the two groups (p < 0.05).Conclusion: Fufang xueshuantong capsule is effective in the treatment of diabetic retinopathy, and significantly improves ophthalmic artery hemodynamics. Further clinical trials, however, are required for the validation of the findings of this study.
{"title":"Comparative effect of laser therapy and Fufang xueshuantong capsules in patients with diabetic retinopathy and ocular hemodynamic characteristics","authors":"Xinming Peng, Peng Chen, Yiwen Liu, Yajing Lv, Guang-yu Chen, Jing Wang","doi":"10.4314/tjpr.v22i7.19","DOIUrl":"https://doi.org/10.4314/tjpr.v22i7.19","url":null,"abstract":"Purpose: To investigate the therapeutic effect of combined administration of laser and Fufang xueshuantong capsules in patients with diabetic retinopathy, and its influence on ocular hemodynamics.Methods: A total of 193 patients with diabetic retinopathy in the Ophthalmology Department of Hebei University Affiliated Hospital from October 2020 to April 2022 were retrospectively analyzed. The patients were divided into two groups: study group (127 patients) and control group (66 patients). Control group received laser therapy in the fundus while the study group was administered 3 capsules (i.e., 1.5 g) of Fufang xueshuantong thrice daily for 7 days. Changes in the fundus and visual acuity, hemodynamic indices of the ophthalmic artery, and changes in macular edema diameter, and adverse reactions in both groups were recorded.Results: In the study group, after treatment, eyeground changes were especially effective (67.37 %), effective (30.51 %), or ineffective (2.12 %). Visual acuity improved (58.90 %), (unchanged at 38.56 %), or decreased (2.54 %). In the control group, laser treatment was especially effective (42.61 %), effective (44.35 %), or ineffective (13.04 %); however, visual acuity increased (36.52 %), unchanged (47.83 %), or decreased (15.65 %). The changes were significant (p < 0.05). There was no significant difference in ophthalmic artery hemodynamics between the two groups before treatment (p > 0.05). After treatment, however, peak systolic velocity (Psv), end diastolic blood flow velocity (Edv), mean velocity (Vm), resistance index (RI), and pulsatile index (PI) in the study group were significantly different from those of the control group (p < 0.05); There was also no significant difference between the two groups before and 1 day after the operation (p > 0.05), neither was there a significant difference in adverse reactions between the two groups (p < 0.05).Conclusion: Fufang xueshuantong capsule is effective in the treatment of diabetic retinopathy, and significantly improves ophthalmic artery hemodynamics. Further clinical trials, however, are required for the validation of the findings of this study. ","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"110 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80319658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ying Yang, Yuan Xie, Xinna Zhao, Meiyan Qi, Xuebo Liu
Purpose: To investigate the effect of pogostone on cardiac hypertrophy.Methods: An in vitro model of myocardial hypertrophy was first established by stimulating H9c2 (rat cardiomyocytes) with angiotensin II (Ang II), and the cells treated with or without pogostone. Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) were measured by western blot. Immunofluorescence staining was performed for α-actinin while cell surface area was quantified. Dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe and malondialdehyde (MDA) assay kit were used to determine reactive oxygen species (ROS) and MDA levels respectively. Apoptosis was evaluated by flow cytometry while Nrf2, p38, ERK, and JNK protein expression levels were determined by western-blot assay.Results: Compared with the control group, ANP and BNP protein expression levels, cell surface area, ROS, MDA, and apoptosis were all elevated in H9c2 cells after stimulation with Ang II (p < 0.001). Varying doses of pogostone decreased protein expressions of ANP and BNP, reduced cell surface area, decreased ROS and MDA levels, and inhibited apoptosis. Pogostone also up-regulated and inhibited the phosphorylation levels of p38 and ERK, and JNK levels in H9c2 cells.Conclusion: Pogostone reduces protein expression of ANP and BNP and up-regulated Nrf2 protein expression in H9c2 cells stimulated with angiotensin II.
{"title":"Pogostone alleviates angiotensin II-induced cardiomyocyte hypertrophy in H9c2 cells through MAPK and Nrf2 pathway","authors":"Ying Yang, Yuan Xie, Xinna Zhao, Meiyan Qi, Xuebo Liu","doi":"10.4314/tjpr.v22i7.3","DOIUrl":"https://doi.org/10.4314/tjpr.v22i7.3","url":null,"abstract":"Purpose: To investigate the effect of pogostone on cardiac hypertrophy.Methods: An in vitro model of myocardial hypertrophy was first established by stimulating H9c2 (rat cardiomyocytes) with angiotensin II (Ang II), and the cells treated with or without pogostone. Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) were measured by western blot. Immunofluorescence staining was performed for α-actinin while cell surface area was quantified. Dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe and malondialdehyde (MDA) assay kit were used to determine reactive oxygen species (ROS) and MDA levels respectively. Apoptosis was evaluated by flow cytometry while Nrf2, p38, ERK, and JNK protein expression levels were determined by western-blot assay.Results: Compared with the control group, ANP and BNP protein expression levels, cell surface area, ROS, MDA, and apoptosis were all elevated in H9c2 cells after stimulation with Ang II (p < 0.001). Varying doses of pogostone decreased protein expressions of ANP and BNP, reduced cell surface area, decreased ROS and MDA levels, and inhibited apoptosis. Pogostone also up-regulated and inhibited the phosphorylation levels of p38 and ERK, and JNK levels in H9c2 cells.Conclusion: Pogostone reduces protein expression of ANP and BNP and up-regulated Nrf2 protein expression in H9c2 cells stimulated with angiotensin II.","PeriodicalId":23347,"journal":{"name":"Tropical Journal of Pharmaceutical Research","volume":"491 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80402767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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