Veterinarni Medicina最新文献_第6页

Lactobacillus sp. participated in the adaptation of Rongchang piglets to cold stress 乳酸菌参与了荣昌仔猪对冷应激的适应

4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina

Pub Date : 2023-10-24 DOI: 10.17221/54/2023-vetmed

J Chai, X Long, P Wu, J Wang, X Wu, Z Tu, M Wei, Z Guo, T Zhang, L Chen

Rongchang piglets were easily induced toÂ cold stress and diarrhoea inÂ the winter when raised inÂ an open hog house. However, they also gradually recovered under mid-cold stress. Other studies have suggested gut microbiome might beÂ involved inÂ the host energy metabolism toÂ relieve stress. ToÂ study how toÂ adapt Rongchang piglets toÂ cold stress byÂ gut microbiome, thirty Rongchang piglets were randomly divided into aÂ mild cold stress group and aÂ control group for 30Â consecutive days. The findings revealed that the piglets had low growth performance and aÂ high diarrhoea rate and mortality rate during the first half ofÂ the cold treatment, but subsequently stabilised. The level ofÂ cortisol (COR) also displayed aÂ similar trend. InÂ the mild cold stress group, the relative abundance ofÂ Muribaculaceae significantly increased onÂ dayÂ 15, and the predominant bacterial onÂ dayÂ 30 was Lactobacillus sp. Our results indicated that the Rongchang piglet’s production performance and health were impaired atÂ the start ofÂ the mild cold stress. However, asÂ time passed, the body could progressively adapt toÂ the low temperature, and Lactobacillus sp. participated inÂ this process. This study provides new insight into how toÂ alleviate health damage caused byÂ cold stress.

荣昌仔猪在开放式猪舍inÂ饲养时，冬季容易诱发toÂ冷应激和腹泻inÂ。然而，在中低温胁迫下，它们也逐渐恢复。其他研究表明，肠道微生物群可能beÂ参与inÂ宿主的能量代谢toÂ缓解压力。ToÂ研究toÂ如何适应荣昌仔猪toÂ冷应激byÂ肠道菌群，将30头荣昌仔猪随机分为aÂ轻度冷应激组和aÂ对照组，连续30Â d。研究结果显示，仔猪的生长性能较低，aÂ腹泻率和死亡率在上半年ofÂ冷处理期间较高，但随后稳定下来。皮质醇水平ofÂ (COR)也表现出aÂ相似的趋势。InÂ轻度冷应激组，相对丰度ofÂ Muribaculaceae显著增加onÂ dayÂ 15，优势菌onÂ dayÂ 30为乳杆菌sp.我们的结果表明，从ofÂ轻度冷应激开始，荣昌仔猪的生产性能和健康受到损害。然而，asÂ随着时间的推移，身体逐渐适应toÂ低温，乳杆菌参与了inÂ这一过程。这项研究为toÂ如何减轻byÂ冷应激造成的健康损害提供了新的见解。

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引用次数: 0

Seroprevalence and haemato-biochemical effects of bovine leucosis in buffalo, Punjab, Pakistan 巴基斯坦旁遮普省水牛白血病的血清患病率和血液生化效应

4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina

Pub Date : 2023-10-24 DOI: 10.17221/57/2023-vetmed

A Rahman, M Kashif, A Nasir, S Ehtisham-ul-Haque, H Ullah, A Sikandar, I Ahmed, AU Rehman, MA Saeed, MW Nazar, M Rizwan, S Saher, A Abbas

Enzootic bovine leucosis isÂ caused byÂ bovine leukaemia virus (BLV), aÂ Deltaretrovirus belonging toÂ the family Retroviridae. BLV causes huge economic losses toÂ the dairy industry inÂ the form ofÂ decreased milk production, premature culling, and poor reproductive performance ofÂ the animals. The aim ofÂ the present study was toÂ determine the seroprevalence ofÂ BLV infection inÂ buffalo inÂ two districts ofÂ Punjab, Pakistan. AÂ total ofÂ 384 samples were collected and analysed using aÂ commercial indirect enzyme-linked immunosorbent assay (ELISA) toÂ investigate the seroprevalence ofÂ BLV through the detection ofÂ the anti-BLV gp51 antibody. AÂ predesigned data questionnaire proforma was employed toÂ find out the association ofÂ risk factors with disease. Overall, 18.2% ofÂ buffaloes were seropositive for BLV inÂ the study population. The results revealed aÂ significant association (PÂ <Â 0.05) ofÂ age with BLV infection. Furthermore, milk yield and pregnancy had aÂ significant association with the seroprevalence ofÂ BLV infection inÂ buffalo whereas noÂ significant association was found with sex, breeding, and health status. Biochemical and oxidative stress markers revealed aÂ significant decrease inÂ liver enzymes alanine transaminase (ALT) and aspartate transaminase (AST), glutathione peroxidase (GPX), and superoxide dismutase (SOD) inÂ seropositive animals asÂ compared toÂ healthy animals. ItÂ is concluded that BLV has aÂ considerable prevalence inÂ buffalo inÂ Punjab, Pakistan and there isÂ aÂ dire need toÂ investigate the disease epidemiology atÂ both national and international levels and strategies should beÂ developed toÂ implement anÂ effective control program.

牛地方性白血病isÂ引起byÂ牛白血病病毒(BLV)， aÂ德尔塔病毒属于toÂ逆转录病毒科。BLV造成巨大的经济损失toÂ乳业inÂ形式ofÂ产奶量下降，过早扑杀，繁殖性能差ofÂ动物。目的ofÂ本研究的目的是toÂ确定血清阳性率ofÂ乙型肝炎病毒感染inÂ水牛inÂ两个地区ofÂ巴基斯坦旁遮普省。AÂ共收集ofÂ 384份样本，采用aÂ商业间接酶联免疫吸附试验(ELISA)进行分析toÂ通过检测ofÂ抗BLV gp51抗体调查ofÂ BLV的血清阳性率。AÂ采用预先设计的数据问卷形式toÂ了解ofÂ危险因素与疾病的关系。总体而言，研究人群中18.2% ofÂ水牛BLV血清呈阳性inÂ。结果显示:aÂ年龄与BLV感染有显著相关性(PÂ <Â 0.05)。此外，产奶量和妊娠与ofÂ BLV感染inÂ水牛血清阳性率有aÂ显著相关，而与性别、繁殖和健康状况有noÂ显著相关。生化和氧化应激指标显示aÂ血清阳性动物与toÂ健康动物相比显著降低inÂ肝脏酶丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、谷胱甘肽过氧化物酶(GPX)和超氧化物歧化酶(SOD) inÂ。ItA得出BLV aA相当流行加载在水牛在旁遮普,巴基斯坦有isA aA急需toA调查疾病流行病学atA国家和国际层面和策略都应该beA发达toA实现安娜有效的控制程序。

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引用次数: 0

The importance of slaughterhouses in monitoring the occurrence of tail biting in pigs - Review 屠宰场监测猪咬尾事件的重要性综述

4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina

Pub Date : 2023-09-29 DOI: 10.17221/85/2023-vetmed

M Svoboda, N Hodkovicová, A Siwicki, W Szweda

Tail biting inÂ pigs represents aÂ very serious problem inÂ modern pig farming, particularly with the intensification ofÂ the industry. ItÂ is considered aÂ multifactorial syndrome and can beÂ caused byÂ various factors, leading toÂ significant economic losses through reduced weight gain and partial orÂ total condemnation ofÂ slaughtered carcasses due toÂ secondary bacterial infections. The aim ofÂ this article isÂ to summarise the current knowledge regarding tail biting inÂ pigs, with aÂ primary focus onÂ the use ofÂ slaughterhouses for evaluating tail lesions and monitoring their prevalence. The introduction addresses the factors influencing the incidence ofÂ tail biting inÂ pig farms and prevention strategies. Subsequent sections cover topics such asÂ tail docking, the negative effects ofÂ pig tail biting, the advantages and drawbacks ofÂ examining tail lesions inÂ slaughterhouses, and the methodical procedure for evaluating such lesions. Additionally, the article discusses the relationship between tail lesions and meat inspection findings, asÂ well asÂ the prevalence ofÂ tail lesions inÂ various European countries.

咬尾inÂ猪代表aÂ非常严重的问题inÂ现代养猪业，特别是随着集约化ofÂ产业。ItÂ被认为是aÂ多因素综合征，可以beÂ引起byÂ多种因素，导致toÂ显著的经济损失，通过减少体重增加和部分orÂ全部谴责ofÂ屠宰的尸体由于toÂ继发细菌感染。目的ofÂ本文isÂ总结目前关于咬尾的知识inÂ猪，aÂ主要关注onÂ使用ofÂ屠宰场评估尾巴损伤和监测其流行情况。介绍了影响发病的因素ofÂ咬尾inÂ猪场及预防策略。随后的章节涵盖了asÂ断尾，负面影响ofÂ猪咬尾，优点和缺点ofÂ检查尾巴病变inÂ屠宰场，以及评估这种病变的系统程序。此外，文章讨论了尾部病变与肉类检验结果的关系，asÂ以及asÂ发病率ofÂ尾部病变inÂ各个欧洲国家。

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引用次数: 0

Retrospective study using biosensor data of a milking Holstein cow with jejunal haemorrhage syndrome 利用生物传感器数据对一头患有空肠出血综合征的荷斯坦奶牛进行回顾性研究

4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina

Pub Date : 2023-09-29 DOI: 10.17221/73/2023-vetmed

S Ha, S Kang, M Jung, E Jeon, S Hwang, J Lee, J Kim, YC Bae, J Park, UH Kim

Jejunal haemorrhage syndrome (JHS) isÂ aÂ sporadic and fatal enterotoxaemic disease inÂ dairy cows associated with acute development and poor prognosis despite treatment. A 5-year-old Holstein cow with noÂ reported pregnancy, three calving numbers, and 303 days inÂ milk presented with hypothermia, discomfort, and inappetence. Anaemia, dehydration, faeces with blood clots, and absence ofÂ rumen and bowel movements were observed. WeÂ identified the presence ofÂ neutrophilia, hyperglycaemia, hypoproteinaemia, azotaemia, hyperlactatemia, hypocalcaemia, hypermagnesemia, hypokalaemia, and hypochloraemia through blood analyses. Necropsy and histopathologic examination revealed aÂ dilated bluish-purple jejunum, blood clots within the jejunum, neutrophil infiltration into the submucosa ofÂ the jejunum, and vascular necrosis. Retrospective examination revealed extraordinary patterns ofÂ rumination time, activity, rumen mobility, and rumen temperature using biosensors and decreased milk yield. The abnormalities inÂ the affected cow were detected before recognition byÂ farm workers. ToÂ the best ofÂ our knowledge, this isÂ the first report toÂ examine data from biosensors inÂ aÂ cow with JHS. Our findings suggest that using biometric data may help understand the development ofÂ JHS.

空肠出血综合征(JHS) isÂ aÂ散发和致命的肠毒血症疾病inÂ与奶牛急性发育和治疗后预后不良相关。一头5岁的荷斯坦奶牛noÂ报告怀孕，产犊3次，产奶303天inÂ，表现为体温过低、不适和食欲不振。观察到贫血，脱水，粪便中有血块，没有ofÂ瘤胃和肠道运动。WeÂ通过血液分析确定了ofÂ中性粒细胞、高血糖、低蛋白血症、氮血症、高乳酸血症、低钙血症、高镁血症、低钾血症和低氯血症的存在。尸检及组织病理学检查显示aÂ空肠蓝紫色扩张，空肠内有血块，ofÂ空肠粘膜下层有中性粒细胞浸润，血管坏死。回顾性检查显示异常模式ofÂ反刍时间、活动、瘤胃流动性和瘤胃温度使用生物传感器和减少的产奶量。异常情况inÂ患病奶牛在被检测到之前就被识别byÂ农场工人。ToÂ最好的ofÂ我们的知识，这isÂ第一份报告toÂ检查数据从生物传感器inÂ aÂ奶牛与JHS。我们的研究结果表明，使用生物识别数据可能有助于了解ofÂ JHS的发展。

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引用次数: 0

Evaluation of Streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genes 亚临床绵羊乳腺炎链球菌的分子鉴定及毒力因子和耐药基因的测定

4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina

Pub Date : 2023-09-29 DOI: 10.17221/42/2023-vetmed

V Ozavci, HT Yuksel Dolgun, S Kirkan, Y Seferoglu, Z Semen, U Parin

Streptococcus (S.) species are important pathogens that cause mastitis inÂ sheep. The study aimed toÂ examine Streptococcus species inÂ sheep milk with subclinical mastitis, assessing their prevalence, antimicrobial resistance, and virulence genes. AÂ total ofÂ 200 milk samples were collected from sheep farms inÂ İzmir’s five districts. Out ofÂ 32 (28.6%) Streptococcus isolates identified byÂ phenotypic methods, 25 were genotypically identified asÂ S.Â uberis, 5Â asÂ S.Â agalactiae, and 2 asÂ S.Â dysgalactiae. Disk diffusion was used toÂ determine the antimicrobial resistance ofÂ the isolates. PCR was employed toÂ identify antimicrobial resistance and virulence genes inÂ the isolates. The highest resistance was found for cloxacillin (100%), and the highest sensitivity was found for florfenicol (84%). The most common resistance gene combination was tetM+tetS (3/32) for S.Â uberis inÂ 9.4%. AÂ total ofÂ five virulence genes were detected. GapC+sua (56.2%) constituted the most common gene pattern. The highest virulence gene gapC was detected inÂ 78.1% (25/32) ofÂ the isolates. The cylE gene was not detected (0%) inÂ the isolates. Streptococcus species may play aÂ role inÂ mastitis inÂ sheep, emphasising the need for meticulous hygienic milking practices.

链球菌(s)种是引起乳腺炎inÂ羊的重要病原体。该研究旨在toÂ检测亚型乳腺炎羊奶链球菌inÂ，评估其患病率、抗菌素耐药性和毒力基因。AÂ共ofÂ从inÂ İzmir的五个区羊场收集了200份牛奶样本。在ofÂ 32株(28.6%)链球菌分离株中，byÂ表型方法鉴定出25株为基因典型鉴定为asÂ S.Â uberis, 5Â asÂ S.Â无乳型和2株asÂ S.Â无乳型。采用纸片扩散法toÂ测定菌株的耐药性ofÂ。采用PCR技术toÂ鉴定菌株的耐药和毒力基因inÂ。耐药最高的是氯西林(100%)，敏感性最高的是氟苯尼考(84%)。S.Â uberis最常见的耐药基因组合为tetM+tetS (3/32) inÂ 9.4%。AÂ共检测到ofÂ 5个毒力基因。GapC+sua(56.2%)是最常见的基因模式。毒力最强的基因为gapC inÂ 78.1% (25/32) ofÂ。未检出cylE基因(0%)inÂ。链球菌种类可能发挥aÂ作用inÂ乳腺炎inÂ羊，强调需要细致卫生挤奶的做法。

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引用次数: 0

Plasma bile acids in healthy green iguanas and iguanas with chronic liver diseases 健康绿鬣蜥和慢性肝病鬣蜥血浆胆汁酸的研究

4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina

Pub Date : 2023-09-29 DOI: 10.17221/10/2023-vetmed

Z Knotek, Z Knotkova, E Cermakova, GM Dorrestein, KO Heckers, D Komenda

The aim ofÂ the study was toÂ establish reference values for plasma bile acid (BA) concentrations inÂ aÂ collection ofÂ healthy green iguanas and toÂ compare the results with BA concentrations inÂ iguana patients presented toÂ the clinic with various types ofÂ chronic liver diseases, patients with other chronic diseases and healthy iguanas that were presented for routine orÂ pre-surgical health check-up. The concentration ofÂ BA was determined using the enzymatic colorimetric method. Mean plasma bile acid concentration inÂ 110 samples from healthy green iguanas fasted for 24Â h was higher (15.89Â ±Â 15.61Â μmol/l) than plasma bile acid concentration inÂ the same iguanas fasted for 48Â h (9.56 ± 8.52Â μmol/l) (PÂ <Â 0.01). The 3α-hydroxy bile acid concentration was significantly altered inÂ 9 patients suffering from chronic liver diseases (diagnosed byÂ histology) (84.85Â ±Â 22.29Â µmol/l). BA concentration inÂ one iguana with hepatocellular adenoma (13.0Â µmol/l) was within the interval ofÂ BA inÂ healthy iguanas. Mean plasma BA concentration inÂ 10Â green iguanas that were suffering from various types ofÂ chronic diseases, but without any hepatopathy was 7.85Â ±Â 4.86Â μmol/l. The mean plasma BA concentration inÂ 18Â samples from green iguanas presented toÂ the clinic for routine health check-ups and 17Â green iguana females with preovulatory follicle stasis (POFS) syndrome presented for ovariectomy was 11.95Â ±Â 9.43Â μmol/l and 12.97Â ±Â 9.06Â μmol/l, respectively. The data collected from this study suggest that plasma bile acids are significantly increased in green iguanas suffering from chronic liver diseases.

的目的研究toA建立等离子胆汁酸引用值(BA)浓度在aA收集耀目健康绿鬣蜥和toA比较结果与BA浓度在鬣蜥患者toA诊所提出各种慢性肝脏疾病,患有其他慢性疾病和健康的鬣蜥,orA术前常规健康检查。用酶比色法测定BA的浓度ofÂ。意味着等离子胆汁酸浓度在110个样本从健康的绿鬣蜥禁食24 h较高(15.89±15.61μmol / l)比等离子胆汁酸浓度在同一鬣蜥禁食48 h(9.56±8.52μmol / l) (PA < 0.01)。3α-羟基胆汁酸浓度显著改变inÂ 9例慢性肝病患者(诊断为byÂ组织学)(84.85Â±Â 22.29Âµmol/l)。BA浓度inÂ 1只肝细胞腺瘤鬣蜥(13.0Âµmol/l)在正常鬣蜥(ofÂ BA inÂ)区间内。患有各种类型ofÂ慢性疾病但无肝脏病变的绿鬣蜥平均血浆BA浓度为7.85Â±Â 4.86Â μmol/l。绿鬣蜥的平均血浆BA浓度inÂ 18Â分别为toÂ常规健康体检的绿鬣蜥样本和17Â卵巢切除术时出现排卵前卵泡淤积(POFS)综合征的绿鬣蜥样本，分别为11.95Â±Â 9.43Â μmol/l和12.97Â±Â 9.06Â μmol/l。本研究收集的数据表明，患有慢性肝病的绿鬣蜥血浆胆汁酸显著增加。

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引用次数: 0

Incidence of aflatoxin M1 in cows' milk in Pakistan, effects on milk quality and evaluation of therapeutic management in dairy animals 巴基斯坦牛奶中黄曲霉毒素M1的发病率、对牛奶质量的影响以及奶牛治疗管理的评价

4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina

Pub Date : 2023-06-30 DOI: 10.17221/18/2023-vetmed

I Ullah, A Nasir, M Kashif, A Sikandar, M Sajid, M Adil, A Rehman, MU Iqbal, H Ullah

The present study was aimed at measuring the concentration of aflatoxin M1 (AFM1) in the milk of Holstein Friesian cows, its effect on the milk quality and seasonal trends, as well as to investigate the efficacy of a commercial clay-based toxin binder. For this purpose, milk samples from dairy cows (n = 72) were collected and assayed for AFM1 before employing a clay-based toxin binder. The milk samples (n = 72) were collected from selected animals, revealing that 69.4% of the milk samples had AFM1 levels above the United States permissible limit (0.5 µg/kg). The incidence of AFM1 in milk during the winter and summer was 82.5% and 53.1%, respectively. Owing to the presence of AFM1, the level of milk fat, solids-not-fat, and protein were found to be low. Subsequently, the affected animals were divided into two groups, i.e., AFM1 positive control (n = 10) and the experimental group (n = 40). The experimental group of animals were fed the clay-based toxin binder at 25 g/animal/day. A progressive decrease of 19.8% in the AFM1 levels was observed on day 4 and on day 7 (53.6%) in the treatment group. Furthermore, the fat, solids-non-fat and protein increased significantly in the milk. In conclusion, a high level of AFM1 contamination occurs in the milk in Pakistan, affecting the quality of the milk production. Clay-based toxin binders may be used to ensure the milk quality and to protect the animal and consumer health.

本研究旨在测定荷斯坦黑黑马奶牛牛奶中黄曲霉毒素M1 (AFM1)的浓度、对牛奶品质的影响和季节变化趋势，并研究一种商业化粘土基毒素粘合剂的效果。为此，从奶牛(n = 72)收集牛奶样本，并在使用粘土基毒素粘合剂之前检测AFM1。从选定的动物中收集的牛奶样本(n = 72)显示，69.4%的牛奶样本的AFM1水平高于美国允许限量(0.5µg/kg)。冬季和夏季牛奶中AFM1的发病率分别为82.5%和53.1%。由于AFM1的存在，牛奶脂肪、非脂肪固体和蛋白质的水平都很低。随后将染病动物分为两组，即AFM1阳性对照组(n = 10)和实验组(n = 40)。试验组动物以25 g/只/d的剂量饲喂粘土基毒素黏合剂。治疗组AFM1水平在第4天和第7天进行性下降19.8%(53.6%)。此外，牛奶中的脂肪、非脂肪固体和蛋白质含量显著增加。总之，巴基斯坦的牛奶中发生了高浓度的AFM1污染，影响了牛奶生产的质量。粘土基毒素粘合剂可用于保证牛奶质量，保护动物和消费者的健康。

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引用次数: 0

In vitro evaluation of the composition and acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mites. 荨麻叶乙酸乙酯提取物对疥螨的体外杀螨效果及成分评价

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina

Pub Date : 2023-05-24 eCollection Date: 2023-05-01 DOI: 10.17221/6/2023-VETMED

Fei Liao, Taotao Bao, Guangyao Tao, Yanchun Hu, Changquan Han

In veterinary medicine, natural products provide an alternative to chemical agents for mite management. In the present study, the acaricidal efficacy of Urtica fissa leaf ethyl acetate extract against Sarcoptes scabiei mites was examined. The chemical composition of the extract was determined using liquid chromatography-mass spectrometry (LC-MS) analysis. The ethyl acetate extract was found to be extremely toxic to mites at a concentration of 100 mg/ml (m/v), killing all S. scabiei within two hours. The median lethal time (LT50) values for ethyl acetate extract concentrations of 25, 50, and 100 mg/ml against S. scabiei were 1.706, 1.204, and 0.750 h, respectively. The median lethal dosage (LC50) for S. scabiei was 19.14 mg/ml at two hours. The chemical composition of the ethyl acetate extract was evaluated using LC-MS, showing that the major components were schaftoside (8.259%), carnosol (6.736%), prostaglandin A2 (5.94%), 13(S)-HpOTrE (4.624%), nandrolone (4.264%), 1H-indole-3-carboxaldehyde (4.138%), 9-oxoODE (3.206%), and stearidonic acid (2.891%). In conclusion, these findings indicate that Urtica fissa contains promising new acaricidal compounds capable of successfully controlling animal mites.

在兽医学中，天然产品为螨虫管理提供了化学药剂的替代品。本研究考察了荨麻叶乙酸乙酯提取物对疥螨的杀螨效果。采用液相色谱-质谱(LC-MS)分析方法测定提取物的化学成分。发现乙酸乙酯提取物在浓度为100 mg/ml (m/v)时对螨虫极具毒性，可在2小时内杀死所有疥螨。25、50和100 mg/ml乙酸乙酯提取物对金黄色葡萄球菌的中位致死时间(LT50)分别为1.706、1.204和0.750 h。2 h的中位致死剂量(LC50)为19.14 mg/ml。采用LC-MS对乙酸乙酯提取物的化学成分进行鉴定，主要成分为schaftoside(8.259%)、carnosol(6.736%)、前列腺素A2(5.94%)、13(S)-HpOTrE(4.624%)、诺龙酮(4.264%)、1h -吲哚-3-甲醛(4.138%)、9-oxoODE(3.206%)和硬脂酸(2.891%)。综上所述，这些发现表明，荨麻含有有希望的新型杀螨化合物，能够成功地控制动物螨。

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引用次数: 0

The impact of urine storage methods on the results of quantitative bacterial culture in dog and cat. 尿液储存方法对犬、猫定量细菌培养结果的影响。

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina

Pub Date : 2023-03-30 eCollection Date: 2023-03-01 DOI: 10.17221/111/2022-VETMED

Ching-Jung Lien, Shang-Lin Wang

Quantitative bacterial culture (QBC) is the gold standard for determining urinary tract infections. However, the majority of urine samples were sent to a lab for further bacterial culture. Therefore, the storage condition was vital to maintain the quality and accuracy of the urine samples. The main objective of our study was to examine the urine QBC outcomes of (1) immediate culture, (2) culture after urine sample storage at ambient temperature for 24 h, and (3) culture after urine sample storage at 4 °C for 24 hours. There were 49 feline samples and 30 canine samples included in this study. All QBC samples kept at ambient temperature and refrigerator were consistent with immediate QBC in cats. Eight positive results from immediate QBC were in accordance with refrigerated results in dogs. There were ten positive results in the room-temperature sample with two false-positive results. Our study showed that storing conditions at room temperature or refrigeration for 24 h does not impact the results of QBC in cat urine samples. For dog samples, chilled samples have a higher accuracy rate than room temperature samples, although the overall agreement was still satisfactory.

定量细菌培养(QBC)是检测尿路感染的金标准。然而，大多数尿液样本被送到实验室进行进一步的细菌培养。因此，保存条件对保持尿样的质量和准确性至关重要。我们研究的主要目的是检查(1)即时培养，(2)尿液样本在室温下储存24小时后的培养，以及(3)尿液样本在4°C下储存24小时后的培养。本研究共纳入49个猫科动物样本和30个犬类样本。所有在室温和冰箱中保存的QBC样品与猫的即时QBC一致。即刻QBC的8个阳性结果与冷藏犬的结果一致。室温样品有10个阳性结果，2个假阳性结果。我们的研究表明，在室温或冷藏24小时的条件下，猫尿样品中的QBC结果不受影响。对于狗的样本，冷冻样本的准确率高于室温样本，尽管总体上的一致性仍然令人满意。

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引用次数: 0

Corrigendum: The effect of brown seaweed and polyphenol supplementation in male rabbits on the blood profile and antioxidant markers 勘误:褐藻和多酚补充剂对雄性家兔血液特征和抗氧化标志物的影响

4区农林科学 Q3 VETERINARY SCIENCES

Veterinarni Medicina

Pub Date : 2023-01-19 DOI: 10.17221/4/2023-vetmed

M Massanyi

引用次数: 0