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Characterization of optical parameters of breast cancercell line - BT474 by polarimetry technique 用偏振法测定乳腺癌细胞系BT474的光学参数
Pub Date : 2019-09-16 DOI: 10.31276/vjste.61(3).25-32
Thanh T. Nguyen, Minh-Vy Huynh, Thanh-Hai Le, T. Pham
Breast cancer is a well-known health issue that has been a major focus for healthcare professionals for quite some time. Still, the most common noninvasive diagnostic tool - mammography - results in a high false positive rate along with risks of exposure to radiation. These disadvantages are magnified and become more severe when screenings are done repeatedly. To tackle this problem, we introduce a novel framework for uncomplicated diagnosis of breast cancer. Our method utilizes the analytical technique of Mueller matrix decomposition and Stokes vector polarimetry from a polarized light system consisting of a helium-neon laser (wavelength of 632.5 nm), a quarter-wave plate, polarizers, and a Stokes polarimeter. Thus, this technique introduces no radiation. We extracted nine optical parameters of a breast cancer cell line - BT474 - and determined the relationshipand separation power of these parameters to cancerous cells and healthy cells. Specifically, the samples were designed as a two-dimensional cellular model of malignant breast tumours that combined a range of four cell densities - 104, 105, 106, and 107 cells - per an area of 9 cm2.Nine optical parameters - orientation angle of linear birefringence (α), retardance or linear birefringence (β), optical rotation angle or circular birefringence (γ), orientation angle of linear dichroism (θd), linear dichroism (D), circular dichroism (R), degrees of linear depolarization (e1 and e2), and degree of circular depolarization (e3) - were extracted from a total of 40 samples using the polarized light system. The results revealed the positive correlations between three cell densities (104, 105, and 106) and the orientation angle of linear birefringence (R2 = 0.8038), linear birefringence (R2 = 0.8627), and linear dichroism (R2 = 0.9662). Meanwhile, both the orientation angle of linear dichroism and circular dichroism illustrated the negative correlation with that range of cell densities with R2 = 0.9983 and 0.9447, respectively. This proves that the optical parameters measured demonstrate significant association with the cells’ characteristics and thus, the proposed method could pave the way for an accessible diagnosis of breast cancer.
乳腺癌是一个众所周知的健康问题,很长一段时间以来一直是医疗保健专业人员关注的主要问题。尽管如此,最常见的非侵入性诊断工具——乳房x光检查——导致高假阳性率以及暴露于辐射的风险。当反复进行筛查时,这些缺点会被放大并变得更加严重。为了解决这个问题,我们引入了一个简单诊断乳腺癌的新框架。我们的方法利用Mueller矩阵分解和Stokes矢量偏振法的分析技术,从一个由氦氖激光器(波长为632.5 nm)、四分之一波片、偏振器和Stokes偏振计组成的偏振光系统中获得。因此,这种技术不会产生辐射。我们提取了乳腺癌细胞系BT474的9个光学参数,并确定了这些参数与癌细胞和健康细胞的关系和分离能力。具体来说,这些样本被设计成恶性乳腺肿瘤的二维细胞模型,它结合了四种细胞密度的范围——每9平方厘米的面积上有104、105、106和107个细胞。从40个样品中提取了线性双折射取向角(α)、延迟或线性双折射取向角(β)、旋光角或圆双折射取向角(γ)、线二色取向角(θd)、线二色取向角(D)、圆二色取向角(R)、线去极化度(e1和e2)、圆去极化度(e3)等9个光学参数。结果表明,细胞密度(104、105、106)与线性双折射取向角(R2 = 0.8038)、线性双折射取向角(R2 = 0.8627)、线性二色性取向角(R2 = 0.9662)呈正相关。同时,线二色取向角和圆二色取向角与细胞密度范围呈负相关,R2分别为0.9983和0.9447。这证明了测量的光学参数与细胞的特征有显著的关联,因此,提出的方法可以为乳腺癌的诊断铺平道路。
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引用次数: 0
Partitioning of fluoxetine into mixed lipid bilayer containing 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) 氟西汀在含有1,2-二棕榈酰-sn-甘油-3-磷酸甘油(DPPG)和1,2-二硬脂酰-sn-甘油-3-磷酸胆碱(dsc)的混合脂质双分子层中的分配
Pub Date : 2019-09-16 DOI: 10.31276/vjste.61(3).16-24
Anh T. Sy, Vy T. H. Pham, T. Nguyen
In this study, the partitioning of fluoxetine, an antidepressant of selective serotonin reuptake inhibitor class into a mixture containing anionic and zwitterionic lipid vesicles was evaluated using second derivative spectrophotometry. The partition coefficients (Kp) of fluoxetine into the large unilamellar vesicles (LUVs) composed of zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) containing 0 mol%, 10 mol%, 20 mol%, and 30 mol% of anionic 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG) were measured in HEPES buffer at pH 7.4. The result revealed that when more negatively charged lipids incorporated into the LUVs, the condensing effect on the binary phospholipid membrane impeded the partitioning of positively charged fluoxetine, resulting in the decrease in the Kpvalues. This study adds a deeper understanding of how antidepressant fluoxetine exerts its effect on anionic-containing biological membranes, shedding light onto drug delivery systems in the pharmaceutical field.
在这项研究中,氟西汀是一种选择性5 -羟色胺再摄取抑制剂类抗抑郁药,用二阶导数分光光度法评估了氟西汀在含有阴离子和两性离子脂质囊泡的混合物中的分配。在pH 7.4的HEPES缓冲液中,测定了氟西汀在含有0、10、20、30 mol%阴离子1、2-二硬脂酰-sn-甘油-3-磷酸甘油(DPPG)的两性离子1、2-二硬脂酰-sn-甘油-3-磷酸甘油(DPPG)的大单层囊泡(LUVs)中的分配系数(Kp)。结果表明,当更多带负电荷的脂质加入到luv中时,对二元磷脂膜的凝聚作用阻碍了带正电荷的氟西汀的分配,导致kp值降低。这项研究加深了对抗抑郁药氟西汀如何对含阴离子的生物膜发挥作用的理解,为制药领域的药物输送系统提供了新的思路。
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引用次数: 0
The current state of domestic water consumption and the feasibility of implementing a rainwater harvesting system in the coastal zone of the Vietnamese Mekong delta: the case study of the Vinh Chau town, Soc Trang province 越南湄公河三角洲沿海地区生活用水现状及实施雨水收集系统的可行性:以上庄省荣洲镇为例
Pub Date : 2019-09-16 DOI: 10.31276/vjste.61(3).90-96
N. Nguyen, T. Tran, Van Der Heiden Dion, D. Dinh, P. Van
The research was conducted to assess the feasibility of the installation of a rainwater harvesting system for households facing difficulties in using tap water and groundwater for domestic purposes in a coastal area of the Vietnamese Mekong delta - the case study of the Vinh Chau town, Soc Trang province. Direct household interviews and literature review were executed to comprehend the current state of domestic water consumption. According to the research results, Vinh Chau was among the towns seeing moderate rainfall in the delta (~ 1,776 mm/year), and rainwater was used frequently by the local people. However, local households did not employ proper harvesting techniques, hence the harvested rainwater did not meet the required quality for domestic use. Additionally, difficulties in using tap water, groundwater, and surface water were notable. The quality of tap water was not suitable for household consumption because of the presence of aluminum, and the price was high relative to the household’s income. Salinization of surface and groundwater, as well as degradation in quantity of ground water, made water of acceptable quality scarce. The research results also indicated that the use of rainwater harvested by a proper system demonstrated a higher cost efficiency than that of other water resources. Therefore, the installation of a rainwater harvesting system for water supply should be encouraged in order to provide a safe and efficient alternative water source for domestic use, and to contribute to the relief of domestic water-related issues and pressure on groundwater extraction in the study area.
这项研究是为了评估在越南湄公河三角洲沿海地区为家庭使用自来水和地下水遇到困难的家庭安装雨水收集系统的可行性——以越南上庄省永洲镇为例。采用直接入户访谈和文献回顾的方法了解家庭用水现状。根据研究结果,荣洲是三角洲地区雨量适中的城镇之一(约1776毫米/年),当地居民经常使用雨水。然而,当地家庭没有采用适当的收集技术,因此收集的雨水不符合家庭使用的要求。此外,使用自来水、地下水和地表水的困难也很明显。由于铝的存在,自来水的质量不适合家庭消费,并且相对于家庭收入而言价格较高。地表水和地下水的盐碱化,以及地下水数量的减少,使得质量可以接受的水稀少。研究结果还表明,利用适当系统收集的雨水比利用其他水资源具有更高的成本效益。因此,应鼓励安装雨水收集供水系统,以便为家庭使用提供一种安全和有效的替代水源,并有助于缓解与家庭用水有关的问题和对研究地区地下水开采的压力。
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引用次数: 0
Initial characterisation of exosomes released by umbilical cord-derivedmesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions 脐带源性间充质干细胞和成熟树突状细胞在“良好生产规范”条件下释放外泌体的初步表征
Pub Date : 2019-09-16 DOI: 10.31276/vjste.61(3).45-51
Hoang Huong Diem, Bui Thi Van Khanh, H. Nhung, N. Liêm, T. Uyen
Exosomes represent an important mode of intercellular communication and play key roles in many physiological and pathological processes. Exosomes have hitherto exhibited their capacity to modulate biological activities through their carrying of functional molecules such as proteins, lipids, and genetic materials. In the current study, we investigated exosomes released by mature dendritic cells (mDCs) and umbilical cord-derived mesenchymal stem cells (UCMSCs) under Good Manufacturing Practice (GMP) conditions. Ultracentrifugation was used to isolate and purify the exosomes. Additionally, a transmission electron microscope (TEM) and immunoblotting were used to characterise exosomal morphology and markers. The preliminary results showed that both mDCs and UCMSCs secreted exosomes into GMP culture media. Exosomes exhibited a cup-shaped morphology and showed positive for CD63. Additionally, no difference was observed between mDC-derived exosomes and UCMSC-derived exosomes regarding marker expression or morphology. This data indicates the potential for further development of GMP exosomes for clinical application.
外泌体是细胞间通讯的重要方式,在许多生理和病理过程中起着关键作用。迄今为止,外泌体通过携带功能分子(如蛋白质、脂质和遗传物质)显示出调节生物活动的能力。在本研究中,我们研究了成熟树突状细胞(mDCs)和脐带源性间充质干细胞(UCMSCs)在良好生产规范(GMP)条件下释放的外泌体。采用超离心分离纯化外泌体。此外,透射电子显微镜(TEM)和免疫印迹法用于表征外泌体形态和标记物。初步结果表明,mDCs和UCMSCs均在GMP培养基中分泌外泌体。外泌体呈杯状,CD63阳性。此外,mdc衍生的外泌体和ucmsc衍生的外泌体在标记物表达或形态上没有差异。这一数据表明GMP外泌体有进一步开发用于临床应用的潜力。
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引用次数: 0
Synthesis and characterisation of dysprosium-doped borate glasses for use in radiation dosimeters 辐射剂量计用掺镝硼酸玻璃的合成与表征
Pub Date : 2019-09-16 DOI: 10.31276/vjste.61(3).03-08
R. Omar
This paper reports on the synthesis and characterisation of Dy2O3-doped magnesium borate (MB) glasses containing different modifiers, lithium, calcium, and sodium oxides. Glasses composed of (70-z)B2O3-20Li2O; CaO; Na2O-10MgO-zDy2O3 (where 0.05≤z≤0.7 mol%) were prepared using the melt-quenching method. X-ray diffraction (XRD) pattern of the as-quenched samples verified their amorphous character. Differential thermal analysis (DTA) confirmed excellent glass-forming ability and thermal stability in the range of 0.60-0.67 and 0.18-0.82, respectively. The energy dispersive X-ray (EDX) spectra verified the precise elemental traces in the studied glasses. Furthermore, MB glasses doped with 0.1 mol% of Dy2O3 and modified with lithium oxide were found to have the best soft tissue equivalence (Zeff≈8.13). In short, the proposed MB glass system doped with dysprosium ions (Dy3+) was established as effective for accurate radiation detection in emergency situations.
本文报道了含有不同改性剂、锂、钙、氧化钠的dy2o3掺杂硼酸镁玻璃的合成和表征。(70-z)B2O3-20Li2O玻璃;曹;采用熔淬法制备了Na2O-10MgO-zDy2O3(0.05≤z≤0.7 mol%)。x射线衍射(XRD)图证实了其非晶性。差热分析(DTA)证实该材料具有良好的玻璃形成能力,热稳定性在0.60 ~ 0.67和0.18 ~ 0.82之间。能量色散x射线(EDX)光谱证实了所研究玻璃中精确的元素痕迹。此外,掺入0.1 mol% Dy2O3并用氧化锂修饰的MB玻璃具有最佳的软组织等效性(Zeff≈8.13)。总之,所提出的掺杂镝离子(Dy3+)的MB玻璃体系可以有效地在紧急情况下进行精确的辐射检测。
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引用次数: 0
On the melting of interstitial alloys FeH, FeSi and FeC with a body-centred cubic structure under pressure 具有体心立方结构的FeH、FeSi和FeC间质合金在压力下的熔化
Pub Date : 2019-06-30 DOI: 10.31276/VJSTE.61(2).17-22
N. Hoc, N. Hoa, Tran Dinh Cuong, D. Thang, Hanoi Vietnam Communications
Taking the model of interstitial alloy AB with a body-centred cubic structure and the condition of absolute stability for the crystalline state, we derive analytic expression for the temperature of the limit of absolute stability for the crystalline state, the melting temperature, and the equation for the melting curve of this alloy using the statistical moment method. The results allow us to determine the melting temperature of alloy AB under pressure as well as at zero pressure. In limit cases, we obtain the melting theory of main metal A with a body-centred cubic structure. The theoretical results are numerically applied for alloys FeH, FeSi and FeC using different potentials.
以体心立方结构的间隙合金AB为模型,在晶态绝对稳定的条件下,用统计矩法推导出了该合金的晶态绝对稳定极限温度、熔点和熔点曲线的解析表达式。结果使我们可以确定AB合金在压力和零压力下的熔化温度。在极限情况下,我们得到了主体金属A具有体心立方结构的熔化理论。将理论结果应用于不同电势下FeH、FeSi和FeC合金。
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引用次数: 5
Synthesis and application of graphene aerogel as an adsorbent for water treatment 石墨烯气凝胶水处理吸附剂的合成及应用
Pub Date : 2019-06-13 DOI: 10.31276/VJSTE.61(2).23-28
T. Nguyen, T. Nguyen, H. Tran, M. Nguyen, Huu Hieu Nguyen
In this research, graphene aerogel (GA) was fabricated by chemical reduction method, in which ethylenediamine (EDA) was used as a reducing and functionalising agent. The characterisation of GA was studied by density, field-emission scanning electron microscope, Brunauer-Emmett-Teller (BET) specific surface area, Fourier transform infrared spectroscopy, and X-ray diffraction. The results of the analysis showed that GA exhibits low density, ranging from 4-8 mg/cm3, high porosity, and BET specific surface area changes from 176 to 1845 m2/g. It was found that the suitable content of EDA on the synthesis of GA is 30 μl. The obtained GA was used as an adsorbent for removal of oils and methylene blue (MB) from aqueous solutions. The maximum adsorption capacities of GA for lubricant and crude oils are 160 g/g and 110 g/g respectively. The effecting factors including pH, contact time, and initial concentrations on the adsorption capacity of GA for MB were investigated. The adsorption process of MB onto GA followed the pseudo-second-order kinetic and well-fitted to Langmuir isotherm model. The maximum adsorption capacity for MB from linear Langmuir model was calculated to be 212.76 mg/g at pH 7. Accordingly, GA could be used as a potential adsorbent for removal of oils and MB from water.
本研究以乙二胺(EDA)为还原剂,采用化学还原法制备了石墨烯气凝胶(GA)。采用密度、场发射扫描电镜、BET比表面积、傅里叶变换红外光谱和x射线衍射对GA进行了表征。分析结果表明,GA的密度低,为4 ~ 8 mg/cm3,孔隙率高,BET比表面积为176 ~ 1845 m2/g。结果表明,EDA在GA合成中的适宜含量为30 μl。所得GA作为吸附剂用于去除水溶液中的油脂和亚甲基蓝(MB)。GA对润滑油和原油的最大吸附量分别为160 g/g和110 g/g。考察了pH、接触时间、初始浓度等因素对GA对MB吸附能力的影响。MB在GA上的吸附过程符合拟二级动力学,符合Langmuir等温模型。通过线性Langmuir模型计算,在pH为7时,对MB的最大吸附量为212.76 mg/g。因此,GA可以作为一种潜在的吸附剂用于去除水中的油脂和MB。
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引用次数: 2
Determination of aquaculture distribution by using remote sensing technology in Thanh Phu district, Ben Tre province, Vietnam 利用遥感技术确定越南本特省清富区水产养殖分布
Pub Date : 2019-06-13 DOI: 10.31276/VJSTE.61(2).35-41
N. T. H. Diep, Thitinat Korsem, N. T. Cần, Walaiporn Phonphan, V. Minh
Aquaculture is an important economic activity in the coastal zone of Vietnam. Thanh Phu is one the coastal districts in Ben Tre province that rears brackish aquaculture. In recent years, farmers could not grow shrimp because of salinity intrusion and market price fluctuation. This study aims to determine aquaculture and fallow aquaculture pond distribution by using the three indices of NDVI (Normalized Difference Vegetation Index), MNDWI (Modified Normalized Difference Water Index) and NDBaI (Modified Difference Bareness Index) on Landsat 8 imagery. The results reveal that remote sensing can support the detection of aquaculture and fallow ponds with a high accuracy of 77%. The total aquaculture area is approximately 13,093.65 ha, of which the total fallow area is 581.49 ha (roughly 4.44% of the total aquaculture area). Moreover, the fallow ponds are randomly distributed in all four ecological zones and mostly in the fourth ecological region (about 73.92%). In the fourth region, saline concentration in water is from 20 to 30‰, which directly influences cultured shrimp farms. The results also indicate the spatial distribution of aquaculture ponds and ineffective aquaculture locations using Landsat 8 imagery via index image analysis. The findings support the local management’s decision making on further aquaculture planning.
水产养殖是越南沿海地区一项重要的经济活动。清富是本崔省的沿海地区之一,主要从事微咸水产养殖。近年来,由于盐碱化入侵和市场价格波动,养殖户无法养殖对虾。本研究旨在利用Landsat 8影像上的归一化植被指数(NDVI)、修正归一化水体指数(MNDWI)和修正光秃指数(NDBaI) 3个指数来确定养殖和休耕养殖池塘的分布。结果表明,遥感可以支持水产养殖和休耕池塘的检测,准确率高达77%。水产养殖总面积约13093.65 ha,其中休耕总面积581.49 ha,约占水产养殖总面积的4.44%。休耕池在4个生态区均呈随机分布,其中以第4生态区居多(约占73.92%)。第4区海水含盐量在20 ~ 30‰之间,直接影响对虾养殖场。通过对Landsat 8影像的指数分析,得出了养殖池塘和无效养殖地点的空间分布规律。研究结果支持了当地管理层对进一步水产养殖规划的决策。
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引用次数: 6
A comparison of PM2.5 and PAHs in ambient air between an urban background site and a background site in Ho Chi Minh city 胡志明市城市背景点与背景点环境空气中PM2.5和PAHs的比较
Pub Date : 2019-06-13 DOI: 10.31276/VJSTE.61(2).79-83
T. To, Doan Thien Chi Nguyen, X. Le, Huu Huy Duong
This study aims to evaluate the differences between PM2.5, PAHs concentration, PAHs species, and their gas/particle partitioning in an urban background site as opposed to another background site. Saigon zoo was selected as a representative of the urban background site (UBS), and the Can Gio mangrove reserve was selected as the other background site (BS). PM2.5 and gas and particle-phase PAHs samples were collected at the UBS between March 2017 and February 2018, while the samples were collected at the BS for one week in the dry season (April) and in the rainy season (October). The results demonstrated that both PM2.5 and PAHs concentrations at the UBS were significantly higher than those at the BS. The PM2.5 concentration at the UBS and BS ranged from 13.3 to 67.6 μg/m3 and from 5.1 to 22.7 μg/m3, respectively. The total concentration of 14 PAHs at the UBS was 2.6 times greater than that at BS. Phenanthrene (Phe) was the dominant PAH in ambient air at both sites. Benzo[g,h,i] perylene (BghiP) and Indeno[1, 2, 3-cd]pyrene (InP), which indicate the traffic source, accounted for 3.6 and 3.3% of total PAHs at the UBS, while that at the BS accounted for 1.5 and 1.3%, respectively. This result suggested that vehicular emission strongly affected the UBS site.
本研究旨在评估PM2.5、多环芳烃浓度、多环芳烃种类及其气体/颗粒分布在城市背景站点与其他背景站点之间的差异。城市背景点(UBS)以西贡动物园为代表,另一背景点(BS)以芹焦红树林保护区为代表。2017年3月至2018年2月在UBS收集PM2.5和气体及颗粒相多环芳烃样本,在旱季(4月)和雨季(10月)在BS收集一周样本。结果表明,UBS的PM2.5和PAHs浓度均显著高于BS。UBS和BS的PM2.5浓度分别在13.3 ~ 67.6 μg/m3和5.1 ~ 22.7 μg/m3之间。UBS的14种多环芳烃总浓度是BS的2.6倍。在两个地点的环境空气中,菲(Phe)是主要的多环芳烃。作为交通源的苯并[g,h,i]苝(bhip)和茚二[1,2,3 -cd]芘(InP)在UBS和BS中分别占总PAHs的3.6%和3.3%,而在BS中分别占1.5%和1.3%。这一结果表明,车辆排放对UBS场地的影响很大。
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引用次数: 3
Assessment of hydro-climatological drought conditions for Hong-Thai Binh river watershed in Vietnam using high-resolution model simulation 利用高分辨率模式模拟评估越南洪泰平河流域水文气候干旱条件
Pub Date : 2019-06-13 DOI: 10.31276/VJSTE.61(2).90-96
H. V. Cuong, D. Nam, Trinh Quang Toan
Understating hydro-climatological conditions in a transboundary is always challenging because of issues in sharing available data among riparian countries. The present study has explored the hydro-climatological drought conditions over Hong-Thai Binh river watershed (H-TBRW) based on the downscaled rainfall and reproduced streamflow by the state-of-the-art coupled regional hydroclimate model. The standardized precipitation index (SPI) and streamflow drought index (SDI) indicators are used to define the climatological and hydrological drought conditions, respectively. Both SPI and SDI are derived from the precipitation and streamflow data reproducibility for the H-TBRW during 1950-2015. The results demonstrate a slight increasing trend in both climatological and hydrological conditions. Over the H-TBRW, results reveal that the Da and Thao rivers strongly expect drought conditions; meanwhile, the remaining rivers are very likely to experience similar drought conditions as in the past.
由于在沿岸国家之间共享现有数据的问题,在跨界地区低估水文气候条件总是具有挑战性的。本研究利用最先进的耦合区域水文气候模式,基于缩小尺度的降雨和再现的流量,探讨了洪泰平河流域(H-TBRW)的水文气候干旱条件。标准化降水指数(SPI)和河流干旱指数(SDI)指标分别用于确定气候和水文干旱条件。SPI和SDI均来源于1950-2015年H-TBRW降水和径流数据的再现性。结果表明,气候和水文条件都有轻微的增加趋势。结果表明,在h - thbrw上,达河和邵河强烈预期干旱条件;与此同时,剩下的河流很可能经历与过去类似的干旱状况。
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引用次数: 1
期刊
Vietnam Journal of Science, Technology and Engineering
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