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The effect of silver nanoparticles and preparations of various pharmacological groups on the bactericidal activity of S. enteritidis ATCC 13076. 银纳米颗粒及不同药理学组制剂对肠炎链球菌ATCC 13076杀菌活性的影响。
Pub Date : 2023-06-01 DOI: 10.30917/att-vk-1814-9588-2023-3-16
E. Nefedova
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引用次数: 0
Biological activity of quinoxaline derivatives. 喹诺啉衍生物的生物活性。
Pub Date : 2023-06-01 DOI: 10.30917/att-vk-1814-9588-2023-3-11
A. Klimenko, V. V. Chekrysheva, A. Zubenko, L. Fetisov, A. E. Svyatogorova, E. N. Avagyan
{"title":"Biological activity of quinoxaline derivatives.","authors":"A. Klimenko, V. V. Chekrysheva, A. Zubenko, L. Fetisov, A. E. Svyatogorova, E. N. Avagyan","doi":"10.30917/att-vk-1814-9588-2023-3-11","DOIUrl":"https://doi.org/10.30917/att-vk-1814-9588-2023-3-11","url":null,"abstract":"","PeriodicalId":23579,"journal":{"name":"Veterinaria i kormlenie","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88828793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of the microbiological complex "BONAKA-APK-N" on the quality of pork when feeding feed contaminated with storage molds. 微生物复合物“BONAKA-APK-N”对饲喂受霉菌污染的猪肉饲料质量的影响。
Pub Date : 2023-06-01 DOI: 10.30917/att-vk-1814-9588-2023-3-14
E. Levina, N. Zabashta, E. Golovko, E. Lisovitskaya, I. Sinelshchikova
{"title":"Influence of the microbiological complex \"BONAKA-APK-N\" on the quality of pork when feeding feed contaminated with storage molds.","authors":"E. Levina, N. Zabashta, E. Golovko, E. Lisovitskaya, I. Sinelshchikova","doi":"10.30917/att-vk-1814-9588-2023-3-14","DOIUrl":"https://doi.org/10.30917/att-vk-1814-9588-2023-3-14","url":null,"abstract":"","PeriodicalId":23579,"journal":{"name":"Veterinaria i kormlenie","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83340653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Productivity and indicators of reproductive ability of different breeds at the same conditions of Central Yakutia. 中雅库特不同品种在相同条件下的生产力和繁殖能力指标。
Pub Date : 2023-06-01 DOI: 10.30917/att-vk-1814-9588-2023-3-10
L. Zakharova, N. Chernogradskaya, A. Popova, N. D. Ivanova, R. Ivanov
{"title":"Productivity and indicators of reproductive ability of different breeds at the same conditions of Central Yakutia.","authors":"L. Zakharova, N. Chernogradskaya, A. Popova, N. D. Ivanova, R. Ivanov","doi":"10.30917/att-vk-1814-9588-2023-3-10","DOIUrl":"https://doi.org/10.30917/att-vk-1814-9588-2023-3-10","url":null,"abstract":"","PeriodicalId":23579,"journal":{"name":"Veterinaria i kormlenie","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83847116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new method for obtaining the canine distemper virus antigen for RIHA. 一种获取RIHA犬瘟热病毒抗原的新方法。
Pub Date : 2023-06-01 DOI: 10.30917/att-vk-1814-9588-2023-3-7
A. Elakov, A. Gulyukin, S. Alekseenkova, E. Drozdova, A. V. Kapustin
{"title":"A new method for obtaining the canine distemper virus antigen for RIHA.","authors":"A. Elakov, A. Gulyukin, S. Alekseenkova, E. Drozdova, A. V. Kapustin","doi":"10.30917/att-vk-1814-9588-2023-3-7","DOIUrl":"https://doi.org/10.30917/att-vk-1814-9588-2023-3-7","url":null,"abstract":"","PeriodicalId":23579,"journal":{"name":"Veterinaria i kormlenie","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76660795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioencapsulation methods of Artemia used in feeding sturgeon fish. 青蒿在鲟鱼饲料中的生物胶囊化方法。
Pub Date : 2023-06-01 DOI: 10.30917/att-vk-1814-9588-2023-3-8
A. Elizarova, D. Tyulin, G. Shishanov, I.E. Lippo, A. V. Brigida
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引用次数: 0
Efficiency of the modern drug in case of eimeriosis of chickens of the egg direction in the conditions of a poultry farm with extensive farming. 在粗放型养鸡场条件下,现代药物对鸡蛋方向鸡艾美耳虫病的疗效。
Pub Date : 2023-06-01 DOI: 10.30917/att-vk-1814-9588-2023-3-15
B. M. Makhieva, S. Kabardiev
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引用次数: 0
Clinical and sonographic changes in the uterus of repeatedly over -walking cows with subclinical endometritis 亚临床子宫内膜炎反复过行奶牛子宫的临床和超声改变
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-2
V. Avdeenko, N. Slesarenko, G. S. Nikitin, S. Loshchinin, A. P. Belyakova, E. Shirokova
The research was carried out at a modern high-tech dairy enterprise using robotic milking (GEA DairyProQ JV "Donskoye" of the Volgograd region with the productivity of cows over 15,000 kg for 305 days of lactation. Rectal palpation of the uterus in cows with subclinical endometritis showed the presence of a pronounced cavity, which was 2.78 times higher than that in clinically healthy animals of the same study period, and the thickness of the uterine wall in subclinical endometritis averaged 9.23± 0.21 mm, which was 1.41 times higher than similar data in healthy animals. According to sonography data, the endometrium acquired an echoneodnomogeneous structure, which is caused by the ongoing inflammatory process. Dilated blood vessels are visualized in the myometrium. The revealed zone of reduced density at the border of the muscular and mucous layers is characterized by hyperechogenicity, which indicates signs of developing edema. The study of the perimeter revealed no structural changes. The detailed sonographic signs of subclinical endometritis in repeatedly overworking highly productive cows showed that the endometrial condition did not correspond to normal echographic criteria in more than half of cases in sick cows during estrus. The presence of variable in shape and size areas of increased echogenicity in the horns of the uterus of the M-echo during the hunting period is noted. Hyperechogenic formations in the basal layer of the endometrium were found in every fifth sick cow with signs of subclinical endometritis who repeatedly overdo it, and in every second, with the manifestation of hunting, a clinically healthy animal. The endometrial echostructure was found to be inconsistent with the stage of sexual cycle arousal in cows with subclinical endometritis in comparison with repeatedly over-walking animals without symptoms of uterine pathology. When comparing the obtained linear parameters of the uterus by rectal palpation of its anatomical structures with the parameters of the sonographic data, their correspondence was noted in both study groups. Ultrasound diagnostics provides an objective assessment of the state of the uterus and allows you to differentiate the nature of the pathological process.
该研究是在伏尔加格勒地区的一家现代高科技乳品企业(GEA dairy proq JV“Donskoye”)使用机器人挤奶进行的,奶牛的产奶量超过15,000公斤,泌乳期为305天。亚临床子宫内膜炎奶牛直肠触诊子宫可见明显腔,是同期临床健康动物的2.78倍;亚临床子宫内膜炎奶牛子宫壁厚度平均为9.23±0.21 mm,是健康动物的1.41倍。根据超声资料,子宫内膜获得回声异质结构,这是由持续的炎症过程引起的。肌层可见扩张的血管。肌层和黏液层边界的密度降低区表现为高回声,提示发生水肿的迹象。对周长的研究显示没有结构变化。反复过度工作的高产奶牛的亚临床子宫内膜炎的详细超声征象表明,在发情期超过一半的病牛中,子宫内膜状况不符合正常的超声标准。我们注意到,在子宫角的m回声中存在形状和大小不等的回声增强区。在每5头有亚临床子宫内膜炎迹象的病牛中,每5头有反复过度的子宫内膜炎的病牛中,就会发现子宫内膜基底层的高回声形成;在每一秒钟,有狩猎表现的临床健康动物中,也会发现高回声形成。亚临床子宫内膜炎奶牛的子宫内膜回声结构与无子宫病理症状的反复行走动物的性周期觉醒阶段不一致。将直肠触诊子宫解剖结构所获得的子宫线性参数与超声数据参数进行比较,两个研究组均注意到其对应性。超声诊断提供了子宫状态的客观评估,并允许您区分病理过程的性质。
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引用次数: 0
Clinical and histological characteristics of bursitis in the area of the tarsal joint in cattle. 牛跗骨关节区滑囊炎的临床和组织学特征。
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-20
K. I. Sharykina, S. Pozyabin, E. N. Borhunova, M. Kachalin
Orthopedic pathologies are widespread in cattle (cattle) and cause significant economic damage to livestock. According to the literature, lesions of the limbs of varying severity in dairy cows are recorded in 70% of the livestock. Cattle with orthopedic pathologies lose exercise, their feed intake decreases, which leads to a decrease in their milk and meat productivity. One of the pathologies requiring close attention is aseptic and purulent bursitis of the tarsal joint. Despite the wide distribution of bursitis, the question of effective methods for diagnosing and treating sick animals remains open. This is important, since bursitis tends to progress to a complicated stage, and very often doctors have to deal with purulent bursitis, which can be prevented at an earlier stage of pathology development. In our opinion, when diagnosing and monitoring the treatment of bursitis, it is advisable to use such methods of clinical diagnostics as microscopy of synovial fluid, determination of sensitivity to antibiotics, cytological and histological studies, studies on markers of rheumatoid arthritis, C-reactive protein, diphenylamine test, antistreptolysin- Oh, rheumatoid factor. In addition, it seems relevant to conduct histological studies aimed at improving theoretical knowledge about the pathogenesis of bursitis in the early stages. It should be noted that today the treatment of bursitis by conservative and surgical methods requires large physical and material costs, is lengthy and does not always guarantee a positive result. The paper presents the results of studying the structure and characteristics of orthopedic pathologies in cattle in the Moscow region and information on the prevalence of bursitis. Based on complex studies carried out using clinical, morphological and microbiological methods, the characteristics of reactive and purulent bursitis are presented. In addition, data are presented on the information content of pathomorphological studies and the feasibility of their use in the algorithm for the complex diagnosis of bursitis. The proposed algorithm makes it possible to verify the diagnosis and start treatment in a timely manner, which in turn leads to the preservation of animal productivity.
骨科疾病在牛(牛)中广泛存在,并对牲畜造成重大的经济损失。根据文献记载,70%的奶牛有不同程度的肢体损伤。患有骨科疾病的牛缺乏锻炼,采食量减少,导致产奶和产肉量下降。其中一个需要密切关注的病理是无菌性化脓性滑囊炎的跗骨关节。尽管滑囊炎分布广泛,但诊断和治疗患病动物的有效方法问题仍然存在。这是很重要的,因为滑囊炎往往会发展到一个复杂的阶段,医生经常不得不处理化脓性滑囊炎,这可以在病理发展的早期阶段进行预防。我们认为,在诊断和监测滑囊炎的治疗时,建议采用滑液镜检、抗生素敏感性测定、细胞学和组织学研究、类风湿关节炎标志物研究、c反应蛋白、二苯胺试验、抗溶血素、类风湿因子等临床诊断方法。此外,开展旨在提高对早期滑囊炎发病机制的理论认识的组织学研究似乎是相关的。值得注意的是,目前通过保守和手术方法治疗滑囊炎需要大量的物理和材料成本,时间长,并不能保证总是有积极的结果。本文介绍了研究结果的结构和特征的骨科病理在牛在莫斯科地区和信息的流行滑囊炎。基于临床,形态学和微生物学方法进行的复杂研究,提出了反应性和化脓性滑囊炎的特征。此外,还介绍了病理形态学研究的信息内容及其在滑囊炎复杂诊断算法中使用的可行性。提出的算法使诊断的验证和及时开始治疗成为可能,从而保护动物的生产力。
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引用次数: 0
Strategy of PCR-RFLP genotyping of BLV and its correspondence to phylogenetic classification BLV PCR-RFLP基因分型策略及其与系统发育分类的对应关系
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-4
R. Vafin, Kh.Kh. Gilmanov, P. Shastin, V. Savinov, S. Lopunov, A. Gulyukin
The current classification of Bovine leukemia virus includes 12 genotypes that can be identified by phylogenetic analysis of sequenced nucleotide sequences of the BLV env gene locus, as well as by updated PCR-RFLP genotyping strategies. The purpose of this study was to systematize knowledge about the genetic diversity of Bovine leukemia virus with typing of BLV isolates using the previously developed PCR-RFLP genotyping strategy and assess its consistency with the phylogenetic classification of the studied viral pathogen. The study was carried out in the laboratory of leukemia of the FSBSI FSC VIEV RAS. A bioinformatic search in GenBank NCBI indicates the deposition of nucleotide sequences of the env gene locus from at least 1110 BLV isolates, the genotypic affiliation of which was established by alignment of the analyzed DNA and subsequent phylogenetic analysis. Interpretation of calculated PCR-RFLP profiles of 1110 BLV isolates generated by restriction mapping of the env gene locus for 5 restriction endonucleases (PvuII, SspI, HphI (AsuHPI isoschizomer), HaeIII, and BstYI (BstX2I isoschizomer)) led to an extension of the previously updated genotyping strategy ten new combinations of PCR-RFLP profiles. At the same time, 4 out of 10 new and 2 out of 58 previously described combinations of PCR-RFLP profiles no longer had a genotype-associated status. Thus, the total proportion of genotype-associated combinations of PCR-RFLP profiles was 91.2%, and the proportion of isolates identified by the proposed genotyping strategy with predominantly deposited sequences of the BLV env gene locus in GenBank NCBI was 90,3%. The addition of the previously developed PCR-RFLP genotyping strategy for BLV with two new restriction endonucleases DdeI (BstDEI isoschizomer) and HpyCH4III (Bst4CI isoschizomer) opens the prospect for its full consistency with the phylogenetic classification, which is dynamically developing due to new knowledge about the genetic diversity of Bovine leukemia virus. Additionally, it should be noted that the nucleotide sequences of the env gene locus of 68 of the listed type isolates of 12 known BLV genotypes can be considered as reference in the phylogenetic analysis of the genetic diversity of the representatives of the studied viral pathogen in terms of their genotypic affiliation.
目前牛白血病病毒的分类包括12种基因型,这些基因型可以通过对BLV env基因位点的测序核苷酸序列的系统发育分析以及最新的PCR-RFLP基因分型策略进行鉴定。本研究的目的是利用先前开发的PCR-RFLP基因分型策略对BLV分离株进行分型,从而系统化地了解牛白血病病毒的遗传多样性,并评估其与所研究病毒病原体系统发育分类的一致性。这项研究是在FSBSI FSC VIEV RAS白血病实验室进行的。在GenBank NCBI中进行的生物信息学搜索表明,至少有1110株BLV分离株中存在env基因位点的核苷酸序列,通过分析的DNA比对和随后的系统发育分析确定了其基因型隶属关系。通过对5种限制性内切酶(PvuII、SspI、HphI (AsuHPI同分异构体)、HaeIII和BstYI (BstX2I同分异构体)的环境基因位点进行限制性内切定位,对1110株BLV分离株的计算PCR-RFLP图谱进行了解释,将先前更新的基因分型策略扩展为10种新的PCR-RFLP图谱组合。同时,10个新的PCR-RFLP组合中有4个和58个先前描述的PCR-RFLP组合中有2个不再具有基因型相关状态。因此,PCR-RFLP基因型相关组合的总比例为91.2%,采用该基因型策略鉴定的BLV env基因位点在GenBank NCBI中主要沉积序列的分离株比例为90.3%。由于对牛白血病病毒遗传多样性的新认识,先前开发的含有两种新的限制性内切酶DdeI (BstDEI同分异构体)和HpyCH4III (Bst4CI同分异构体)的BLV PCR-RFLP基因分型策略的加入,为其与系统发育分类的完全一致性开辟了前景。此外,值得注意的是,在列出的12种已知BLV基因型中,68种类型分离物的env基因位点的核苷酸序列可作为所研究病毒病原体代表基因型亲缘关系遗传多样性系统发育分析的参考。
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