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The effectiveness of the use of feed additives "AGROFIT PRO" in the diets of piglets 饲料添加剂“AGROFIT PRO”在仔猪日粮中的应用效果
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-13
Y. Lysenko, A. Shantyz, E. Yeganyan, N. Machneva, R. V. Chus, V.A. Belyak, M. Kerzhner
The article presents the results of studies on the effect of a multi-enzyme feed additive "AGROFIT PRO" on the safety, live weight gain, feed conversion and morpho-biochemical blood parameters of piglets of the rearing group. The investigated additive contains at its core the enzyme phytase and a complex of NPS enzymes, a strain-producer of Penicillium verruculosum, as well as fillers: corn starch; sodium chloride. It is used to improve the digestibility of feed, increase the safety of growth and productivity of farm animals. To set up the experiment, four groups (three experimental and one control) of piglets, 56 heads each, were formed, which were kept in a specialized farm room for the rearing group. During the experiment, all piglets were in identical conditions of keeping and feeding. The research results showed that the safety of piglets in the first and second experimental groups was at the level of 100%, and in the control and third experimental groups it was 96.4%. The increase in the experimental group was 3.8%, 1.2% and 0.3% compared to the control group, and in the experimental groups there was a decrease in feed conversion by 2.1% and 3.4%. Based on the obtained data of the general analysis of the whole blood of piglets, it can be concluded that there are no allergic reactions, pathological and inflammatory processes in the body when giving the experimental animals the studied feed additive. When conducting biochemical studies of blood serum, all the studied parameters were within the intraspecies norms; according to the results of the study, there was no negative effect of the feed additive on the organ systems of the experimental animals under test. A statistically significant difference was recorded between the control and the third experimental group in terms of creatinine, which was higher by 12.9%, and triglycerides by 34.9%.
本文介绍了多酶饲料添加剂“AGROFIT PRO”对饲养组仔猪安全性、活增重、饲料转化率和形态生化血液参数的影响。所研究的添加剂的核心含有植酸酶和NPS酶的复合物,NPS酶是疣状青霉的一种菌株生产者,以及填料:玉米淀粉;氯化钠。用于提高饲料的消化率,提高农场动物的生长安全性和生产力。试验设4组仔猪,每组56头,试验组3头,对照组1头,饲养在饲养组专用的猪场房内。试验期间,所有仔猪均处于相同的饲养和饲养条件下。研究结果表明,第一、第二试验组仔猪的安全性为100%,对照组和第三试验组仔猪的安全性为96.4%。与对照组相比,试验组饲料系数分别提高3.8%、1.2%和0.3%,试验组饲料系数分别降低2.1%和3.4%。根据所获得的仔猪全血一般分析数据,可以得出结论,给实验动物喂食所研究的饲料添加剂时,体内没有出现过敏反应、病理和炎症过程。在进行血清生化研究时,所有研究参数均在种内规范范围内;根据研究结果,该饲料添加剂对实验动物的器官系统没有负面影响。在肌酐和甘油三酯方面,对照组和第三实验组的差异有统计学意义,前者高12.9%,后者高34.9%。
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引用次数: 0
Epizootological situation on particular helminthosis of the carnivores (Canidae) - Arctic fox (Vulpes lagopus) in the territory of Arctic Yakutia 北极雅库特地区食肉动物(犬科)-北极狐(Vulpes lagopus)特有寄生虫的动物流行病学研究
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-8
L. Kokolova, E. Sleptsov, L. Gavrilieva
In this article, the authors present the results of a helminthological study of the Vulpes lagopus in the Arctic territory of Yakutia. On the territory of Yakutia, three species of representatives of the Canidae family live in a wild state - the wolf, the red fox and the Arctic fox. Representatives of the Canidae family are among the most important commercial animals whose fur is highly valued. Over the last decade, the number of fur-bearing animals in the tundra zone of Yakutia has increased significantly. As the authors point out, monitoring of the epizootic situation for especially dangerous Helminthiasis is an integral part of a set of measures to preserve the health of animals and the population in the Arctic zone. The objects of the study are Arctic foxes. Materials for the study were brought from December to March in 2020 from 26, 2021 - 36, 2022 - 162, 2023 - 121 Arctic foxes, carcasses, internal organs and tissues. According to the results of helminthological studies, the composition of helminths was represented by 16 species. The main components of the helminth fauna, in terms of frequency of occurrence and very high rates of invasion intensity, are: the cestode Alveococcus multilocularis, found in 98.1% of the studied Arctic foxes, up to 18,000 ind. of mature Alveococcus in one animal, the nematode Toxascaris leonine occurs in 92.1%, on average up to 34.8±1.6 ind. for one Arctic fox. Also, it was found that the infection of arctic foxes with larvae of Trichinella spiralis is 3%, high intensity of invasion, the cestode Diphyllobothrium latum were infected with up to 5.5% of the examined, on average, 3.5 sp. for one Arctic fox.
在这篇文章中,作者介绍了对雅库特北极地区lagopus的蠕虫学研究结果。在雅库特的领土上,有三种犬科动物的代表——狼、红狐和北极狐——生活在野生状态。犬科动物的代表是最重要的商业动物之一,它们的皮毛价值很高。在过去的十年中,雅库特冻土带的毛皮动物数量显著增加。正如作者所指出的那样,监测特别危险的蠕虫病的动物流行病情况是保护北极地区动物和人口健康的一系列措施的组成部分。这项研究的对象是北极狐。该研究的材料于2020年12月至2020年3月从26日、2021年至36日、2022年至162日、2023年至121只北极狐、尸体、内脏和组织中取出。根据蠕虫学研究结果,该地区的蠕虫组成有16种。从发生频率和入侵强度的角度来看,这些蠕虫区系的主要组成部分是:在所研究的北极狐中有98.1%的多房肺泡球菌,在一只北极狐中有多达18000个成熟肺泡球菌,在92.1%的北极狐中有leonine弓形虫,平均每只北极狐高达34.8±1.6个。另外,北极狐感染旋毛虫幼虫的比例为3%,入侵强度高,被检测的北极狐中有5.5%被感染,平均每只北极狐感染3.5只旋毛虫。
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引用次数: 0
Feed additive "Konyr-su" to increase the productivity of dairy calves 饲料添加剂“Konyr-su”提高奶牛犊牛生产能力
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-10
Yelena Kukhar, К.H. Shaikenova, M.G. Slamiya, N.A. Fogelzan
The article describes the results of research on the development and study of the effect of feed additives on the productivity of cattle, specifically dairy calves. Currently, the issue of increasing the dairy productivity of cows is acute in the Republic of Kazakhstan. The authors have developed a feed additive based on baking yeast and humic substances to increase the dairy productivity of livestock. The composition of the feed additive includes domestic potassium humate produced by NPO Kaztechnougol LLP, obtained from brown coal from the Ekibastuz deposit with content of humic substances up to 56% by dry matter. The components of the nutrient substrate, the yeast brand, the concentration of potassium humate optimizing the accumulation of yeast biomass during cultivation, fermentation parameters, and the cultivation period were selected for the manufacture of the lump additive. For the first time, the effectiveness of the feed additive was tested when used on calves of the dairy period, and its effect on metabolic processes and increasing weight gain was studied. Laboratory tests of the effectiveness of the feed additive on white mongrel mice showed that the live weight of mice in the experimental groups has an average of 27.2 ± 0.4, which is significantly higher than in the control group by 2.8 g. In the production experiment for three months in Kamyshenka LLP on calves of the Holstein-Frisian breed at the age of 2 months, the effectiveness was determined as feed additives for calves. The average daily increase in the experimental group was on average at the level of 895, 856, and 780 g, which is higher than the increase in the control group by 75 g, 126 g, and 130 g, respectively. The live weight of calves of the experimental group increased by 6 kg from the first month of use, by the end of the experiment the difference was 13.1 kg or 7.9%. The revealed trend of increasing weight gain indicates the normalization of metabolism during the drinking of the feed additive "Konyr-su" and in the subsequent period.
本文介绍了饲料添加剂对牛,特别是犊牛生产能力影响的研究进展和研究结果。目前,在哈萨克斯坦共和国,提高奶牛的产奶量是一个紧迫的问题。作者开发了一种以发酵酵母和腐殖质物质为基础的饲料添加剂,以提高牲畜的乳制品产量。饲料添加剂的组成包括NPO Kaztechnougol LLP生产的国产腐植酸钾,从Ekibastuz矿床的褐煤中获得,腐植酸物质的干物质含量高达56%。选择了营养基质成分、酵母品牌、腐植酸钾浓度、发酵参数和培养周期等因素对块状添加剂的生产进行了优化。本试验首次在奶牛期犊牛身上试验了该饲料添加剂的有效性,并研究了其对犊牛代谢过程和增重的影响。该饲料添加剂对白杂种小鼠有效性的实验室试验表明,试验组小鼠的平均活重为27.2±0.4,比对照组显著提高2.8 g。在2月龄荷尔斯坦-弗里斯兰品种犊牛上进行了为期3个月的生产试验,确定了Kamyshenka LLP作为犊牛饲料添加剂的有效性。试验组日平均增加量分别为895、856、780 g,高于对照组75 g、126 g、130 g。试验组犊牛的活重较使用第一个月增加了6 kg,试验结束时差异为13.1 kg或7.9%。所显示的增重趋势表明,在饲料添加剂“科尼苏”饲喂期间及后续阶段,代谢恢复正常。
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引用次数: 0
The relationship of selenium with the productivity of young cattle 硒与犊牛生产能力的关系
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-19
M. Chabaev, R. Nekrasov, M. I. Klementyev, N. Bogolyubova
During the scientific-economic experiment young cattle of the dairy and post-dairy period of growing of the 1st control group (C-) were fed with the basic diet (BD), whereas calves of the 2nd experimental group (C+) with BD feeding additional premix containing sodium selenite (0,30 mg Se per 1 kg of dry matter (DM) of a diet). Animals of the 3rd, 4th and 5th experimental groups (E100, E75, E50) were fed additional 0.30, 0.22 and 0.15 mg Se per 1 kg DM of diet in the form of proteinate. On average over the period of the experiment the total average daily gain (ADG) of body weight of calves in experimental groups was 719; 736; 781; 710 g or 6.8; 9.4; 16; 5.5% more compared to control. Calves from groups E100 and E75 fed 0.30 and 0.22 mg Se used 8.60, 13.75% and 8.56, 13.79% less energy fodder units (EFU) and digestible protein (DP) per 1 kg body weight gain compared to the control group (C-). The animals in the 3rd and 4th experimental groups fed 0.30 and 0.22 mg Se per 1 kg of diet had better digestible dry matter - by 3.18 and 3.92% (p<0.1), organic matter by 1.81% (p<0.1), and 3.64% (p<0.05), protein by 3.93 and 4.01% (p<0.1), fat by 1.02 and 1.80%, fibre by 1.01 and 2.79% (p<0.01), BEV by 1.91 (p<0.1) and 2.05% compared to control. Feeding calves of experimental groups 3, 4 and 5 with various levels of selenium proteinate promoted the increase of selenium concentration in hair (p<0,05) and blood serum (p>0,05) with decrease of Se removal with faeces (p<0,01, E75 vs C-) . In the experimental groups of calves calculation of conditional realization of the additionally gained live weight gain showed a higher level of gain compared to the control by 1481; 2008; 3468,5 and 1200 RUB per head respectively for the period of the experiment.
科学经济试验中,第一对照组(C-)犊牛饲喂基础饲粮(BD),第二对照组(C+)犊牛饲喂亚硒酸钠预混料(每1 kg干物质(DM)添加0.30 mg硒)。第3、4、5试验组(E100、E75、E50)每kg DM饲粮中分别添加0.30、0.22、0.15 mg硒。试验期间,试验组犊牛总平均日增重(ADG)平均为719;736;781;710克或6.8克;9.4;16;比对照组高出5.5%。饲喂0.30和0.22 mg硒的E100和E75组犊牛每千克增重消耗的能量饲料单位(EFU)和可消化蛋白质(DP)分别比对照组(C-)减少8.60、13.75%和8.56、13.79%。饲粮硒含量为0.30和0.22 mg / kg的第3和第4试验组干物质消化率分别提高了3.18%和3.92% (p< 0.05),粪硒去除率降低(p< 0.01, E75 vs C-)。试验组犊牛计算条件实现的额外增重表现出比对照组高1481倍的增重水平;2008;试验期内每头分别饲喂3468、5和1200卢布。
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引用次数: 0
Characteristics of the ichthiofauna reproducing in the middle zone of the Volgograd reservoir in the water areas from 2017 to 2022 2017 - 2022年伏尔加格勒水库中部水域鱼栖动物繁殖特征
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-7
I.A. Kitaev, D. Tyulin, A. V. Brigida, I.E. Lippo
The formation of reservoirs significantly changes all aspects of the life of various fish species, and especially the conditions for their reproduction. Creation of a new reservoir is accompanied by a change in the level and temperature regimes, which in turn leads to shifts in the ecosystem as a whole. The accumulation of significant water masses and their subsequent discharge causes a shift in the seasonal rhythm of the water runoff. With a sharp discharge of water, the spawning area is reduced, up to its complete loss, drying and death of the laid eggs. A favorable level regime, with a high parameters of spring water rise and prolonged standing of water at maximum levels, is rarely observed. For decades, the succession of the Volgograd reservoir has been observed, and at the moment it is not known what the climax point of this process will look like. All this makes it relevant to monitor the reproduction of the ichthyofauna of the reservoir as a whole and for individual water areas. This article presents the results of studies of the qualitative and quantitative composition of the ichthyofauna of the middle zone of the Volgograd reservoir in the waters near Akhmat and Zolotoye villages, Krasnoarmeisk district, Saratov region. The species composition of fish reproducing in the indicated water areas for a six-year period (from 2017 to 2022) and their number in the catches of fry net by years are shown. Dominant, subdominant and specific for each water area species were identified. The fluctuation of species diversity (Shannon index) and the dependence of the state of this indicator on the abundance of individual fish species were studied. The dynamics of the ratio of underyearlings of commercial and non-commercial fish species in catches is considered. Ichthyofauna, reproduced in the waters near the villages Akhmat and Zolotoye in the middle zone of Volgograd reservoir in the period from 2017 to 2022 represented by 21 species of various fish, of which 17 species breed in the water area near Akhmat village and 18 species breed in the water area near Zolotoye village.
水库的形成极大地改变了各种鱼类生活的各个方面,特别是它们的繁殖条件。新水库的建立伴随着水位和温度的变化,这反过来又导致了整个生态系统的变化。大量水团的积累及其随后的排放导致了径流季节节律的变化。随着水的急剧排出,产卵区域减少,直到完全消失,产卵的卵干燥和死亡。很少观测到有利的水位状态,即泉水上升的高参数和最高水位的长时间静止。几十年来,人们一直在观察伏尔加格勒水库的演替过程,但目前还不知道这一过程的高潮将是什么样子。所有这些都使得监测整个水库和个别水域鱼类的繁殖具有重要意义。本文介绍了对萨拉托夫地区克拉斯诺阿尔梅斯克地区Akhmat和Zolotoye村附近水域伏尔加格勒水库中部地区鱼系动物的定性和定量组成的研究结果。显示了6年期间(2017年至2022年)在指定水域繁殖的鱼类种类组成及其按年鱼苗网渔获量。各水域的优势种、亚优势种和特异种均有明确的分类。研究了物种多样性(Shannon指数)的波动及其状态对鱼类个体丰度的依赖关系。考虑了商业和非商业鱼类在渔获量中幼鱼比例的动态变化。2017 - 2022年在伏尔加格勒水库中部Akhmat村和Zolotoye村附近水域繁殖的鱼群,共有21种各种鱼类,其中17种在Akhmat村附近水域繁殖,18种在Zolotoye村附近水域繁殖。
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引用次数: 0
Immunological reactivity in calves obtained from revaccinated cows during immunization with vaccines from B.abortus strains 19, 104M and 82 母牛重新接种牛痘19、104M和82株牛痘免疫后小牛的免疫反应性
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-18
E. Sleptsov, N. Vinokurov, .. T. Rumyantseva
The system of anti-brucellosis measures requires constant improvement in order to increase its effectiveness and reliability. At the same time, the priority remains the creation of high specific protection for brucellosis in animals. Live vaccines from agglutinogenic strains of B.abortus 19, 104M and 82 turned out to be the most immunogenic and effective in antiepizootic relation. As a result of the conducted studies, it was found that the most pronounced general and local reactions were noted in calves vaccinated subcutaneously with a standard dose of vaccine from strain 19, less pronounced in vaccinated with vaccine from strain 104M and weakly in vaccinated with vaccine from strain 82. According to the results of serological studies of blood serum, it can be seen that all animals (100%) reacted in calves for 10-20 days after immunization with vaccines from strains 19 and 104M, and only 40% of calves vaccinated with vaccine from strain 82 reacted. At the same time, calves vaccinated with the strain 19 vaccine reacted in RA in the titers 400-1600 ME. Vaccine from strain 104M - in titers 400-800 ME, vaccine from strain 82 - in titers 25-50 ME. According to the results of serological studies, the state of tolerance was not noted in calves received from re-immunized cows after immunization with agglutinogenic anti-brucellosis vaccines. Further study of the dynamics of antibodies after the introduction of vaccines from strains 19, 104М and 82 by subcutaneous method in a full dose to calves of 3-5 months of age received from re-vaccinated cows showed that the timing of the maximum rise and extinction of the titers of agglutinating and complement-binding antibodies in the blood serum of vaccinated animals is also directly dependent on the properties of the vaccine used. Thus, as a result of serological studies, it was found that the repeated reimunization of pregnant cows with vaccines from strains B.abortus 19, 104M and 82 have no significant effect on the immunological reactivity of the offspring obtained from them, which is confirmed by the absence of a state of tolerance in calves after immunization with agglutinogenic anti-brucellosis vaccines.
需要不断改进抗布鲁氏菌病措施系统,以提高其有效性和可靠性。与此同时,优先事项仍然是为动物布鲁氏菌病建立高度特异性保护。结果表明,产弧弧菌凝集原株19、104M和82活疫苗的免疫原性和抗兽疫效果最好。根据所进行的研究,发现皮下接种标准剂量19株疫苗的犊牛出现最明显的全身和局部反应,接种104M株疫苗的犊牛反应不那么明显,接种82株疫苗的犊牛反应较弱。根据血清血清学研究结果可以看出,接种19株和104M株疫苗后,所有动物(100%)在犊牛中反应10-20天,而接种82株疫苗的犊牛只有40%反应。与此同时,接种19株疫苗的犊牛在400 ~ 1600me滴度之间发生RA反应。104M -株疫苗滴度为400-800 ME, 82 -株疫苗滴度为25-50 ME。根据血清学研究的结果,在接种了凝集原抗布鲁氏菌病疫苗后,再次免疫的奶牛对小牛的耐受状态没有被注意到。对重新接种牛的3-5月龄犊牛皮下注射19、104М和82株全剂量疫苗后抗体动态的进一步研究表明,接种动物血清中凝集抗体和补体结合抗体滴度最大上升和消失的时间也直接取决于所使用疫苗的性质。因此,通过血清学研究,我们发现妊娠奶牛反复接种产弧菌19、104M和82株疫苗对其后代的免疫反应性没有显著影响,这一点得到了犊牛接种凝集原性抗布鲁氏菌疫苗后无耐受状态的证实。
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引用次数: 0
Morphometric parameters of eggs from breeds quail for meat 肉用鹌鹑品种蛋的形态计量参数
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-5
N. German, A. Vetokh, A. Dzhagaev, E.R. Ilyina, T. Kotova
The paper shows the results of assessing eggs quality by morphometric and morphological indicators from meat-type quails, identifying significant differences in the quality indicators of the egg, which are responsible for the incubation and commercial qualities of the egg. In total, 360 eggs from 50 females were tested from Pharaoh and Texas White quails per month. Eggs for this study were collected from the age of 90 days. The shelf life of quail eggs was no more than 2 days from the moment of laying; the storage temperature was not higher than 15 ? at an average air humidity was 75%. The collection of morphological parameters (weight of the whole egg and its components) and biophysical parameters (elastic deformation of the egg, strength and thickness of the shell) was carried out on a highly sensitive analyzer Stable Micro Systems, Ohaus laboratory electronic scales and using a caliper. During the analysis of the eggs, significant differences in the morphological parameters of the eggs were established. The Texas White quail was superior to the Pharaoh quail and had at 2% more elongated shape (at the egg index). In both meat breeds shell thickness was 0.27 mm an average, howewer the difference was found in the shell thickness without an under shell membrane, namely the true shell. From the Texas quail it was 1.12 times thinner than from the Pharaoh quail. The color of the yolk in Texas white quails was more yellow, under the same conditions of keeping and feeding. The quality of protein in quails of both genotypes in our study was high, which indicates their suitability for incubation, and corresponds to good commercial qualities. However, it should be noted that the Pharaoh breed was inferior in HU by 3.78%, in IQU by 4.19%, and the protein index was 1.28 times less than that of the Texas quail.
本文展示了用形态计量学和形态学指标评价肉型鹌鹑蛋品质的结果,发现蛋的品质指标存在显著差异,这些指标决定了蛋的孵化和商品品质。每月总共对50只雌性法老白鹌鹑和德克萨斯白鹌鹑的360个蛋进行检测。本研究的鸡蛋是从90日龄开始收集的。鹌鹑蛋自下蛋之日起保质期不超过2天;贮存温度不高于15℃。平均空气湿度为75%。形态学参数(全蛋重量及其组成部分)和生物物理参数(蛋的弹性变形、蛋壳的强度和厚度)的采集在高灵敏度分析仪Stable Micro Systems、Ohaus实验室电子秤和卡尺上进行。在对卵的分析中,发现卵的形态参数存在显著差异。德克萨斯白鹌鹑优于法老鹌鹑,其长形(蛋指数)高出2%。两个肉用品种的平均壳厚均为0.27 mm,但在无下壳膜即真壳的壳厚上存在差异。德克萨斯鹌鹑比法老鹌鹑细1.12倍。在饲养和饲养条件相同的情况下,德克萨斯白鹌鹑的蛋黄颜色偏黄。本研究中两种基因型鹌鹑的蛋白质质量都很高,表明它们适合孵育,具有良好的商业品质。但值得注意的是,法老品种的HU和IQU分别比德克萨斯鹌鹑低3.78%和4.19%,蛋白质指数比德克萨斯鹌鹑低1.28倍。
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引用次数: 1
Cytotoxic effect of silver and oxide graphene nanoparticles on aberrant forms of Escherichia coli 银和氧化石墨烯纳米颗粒对异常形式大肠杆菌的细胞毒性作用
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-9
R. B. Korochkin, P. Krasochko, M. Ponaskov
The acquisition of aberrant morphological variations by microorganisms can serve as a phenotypic manifestation of antibiotic resistance, which results from ad-aptation to growth in a potentially toxic environment. Therefore, morphogenesis should be considered as an adaptive process that makes a great contribution to the ubiquity and universality of prokaryotes. The aim of the research was to evaluate the antibacterial activity of silver and oxidized graphene nanoparticles on pheno-typically aberrant forms of Escherichia coli. As a result of research, it was found that the antibacterial activity of silver nanoparticles is significantly higher compared to that of oxidized graphene nanoparticles on pleomorphic varieties of Escherichia coli, as the minimum inhibitory concentration of silver nanoparticles is lower (13.1 µg/ml) than that of oxidized graphene nanoparticles (75.3-94.9 µg/ml). The mor-phological variance of E. coli correlated with the acquisition of antibiotic resistance by the bacterium, but was not accompanied by a simultaneous increase in resistance to the nanoparticles. According to the results of atomic force microscopy, the toxic effect of oxidized graphene nanoparticles at supratoxic concentrations on aberrant forms of E. coli did not differ from that on canonical forms of the bacterium and was expressed in the destruction of the bacterial population and filling the intercel-lular space with biomass of degraded bacterial cells.
微生物获得异常形态变异可以作为抗生素耐药性的表型表现,这是由于适应在潜在有毒环境中生长而产生的。因此,形态发生应被认为是一种适应过程,对原核生物的普遍性和普遍性作出了重大贡献。该研究的目的是评估银和氧化石墨烯纳米颗粒对表型异常形式的大肠杆菌的抗菌活性。研究发现,银纳米粒子对多型大肠杆菌的抑菌活性明显高于氧化石墨烯纳米粒子,其最小抑菌浓度(13.1µg/ml)低于氧化石墨烯纳米粒子(75.3 ~ 94.9µg/ml)。大肠杆菌的形态-生理变异与细菌获得抗生素耐药性相关,但不伴随着对纳米颗粒耐药性的同时增加。根据原子力显微镜的结果,氧化石墨烯纳米颗粒在超中毒浓度下对异常形式的大肠杆菌的毒性作用与对典型形式的细菌的毒性作用没有区别,并且表现为细菌种群的破坏和降解细菌细胞的生物量填满细胞间隙。
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引用次数: 0
State of benthic invertebrate communities and water quality in the ponds of Noginsk district of Moscow region 莫斯科州诺金斯克区底栖无脊椎动物群落和池塘水质状况
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-11
I.E. Lippo, D. Tyulin, A. V. Brigida, I.A. Kitaev
Hydrochemical regime is one of the important factors affecting the species composition and the amount of benthic invertebrate communities in a water body. With heavy metal pollution and high eutrophication, the oligochaete index increases, the species diversity of benthic invertebrate communities decreases, and almost all representatives of the entomofauna disappear, with the exception of chironomids. Pollution with phosphates and ammonium nitrogen has an adverse effect on the development of benthic invertebrate communities. At present, the amount of available scientific data on the influence of hydrochemical factors on the development of benthic invertebrate communities in pond farms seems to be insufficient, the focus is on hydrological factors, and this suggests the relevance of new research in this area. The purpose of this study was to compare hydrochemical and temperature indicators with the quantitative and qualitative composition of benthic invertebrate communities in three ponds of Noginsk district of Moscow region in December 2022 - January 2023. The selection of hydrobiological materials and water samples for hydrochemical analysis was carried out in December 2022 - January 2023 in the ponds of Noginsk district of Moscow region in EPB RRIFF, a branch of the Federal State Budgetary Scientific Institution FRC RIAH named after L.K. Ernst. Pond № 1 has an area of 4,48 ha, pond № 2 has an area of 1,7 ha, pond № 3 has an area of 0,61 ha. The studies conducted in December 2022 - January 2023 showed the presence of correlation between water quality (the sum of hydrochemical indicators: concentration of ammonium and nitrate nitrogen, phosphates, total iron, general and carbonate hardness, total mineralization, as well as water temperature) and benthic invertebrate communities (qualitative and quantitative) development indicators. The best indicators of benthic invertebrate communities development were observed in the pond with the best water quality (from a fishery point of view), the worst ones were observed in the pond with the worst water quality (zoobenthos was not found in samples from this pond). In total, 6 taxones of benthic invertebrate communities were found in the studied ponds.
水化学制度是影响水体底栖无脊椎动物群落种类组成和数量的重要因素之一。在重金属污染和高富营养化的环境下,寡毛动物指数上升,底栖无脊椎动物群落物种多样性下降,除手拟虫外,几乎所有昆虫动物群的代表物种都消失了。磷酸盐和铵态氮污染对底栖无脊椎动物群落的发育有不利影响。目前,关于水化学因素对池塘养殖场底栖无脊椎动物群落发展影响的科学数据似乎不足,重点是水文因素,这表明了该领域新研究的相关性。本研究的目的是比较2022年12月至2023年1月莫斯科州诺金斯克区三个池塘的水化学和温度指标与底栖无脊椎动物群落的定量和定性组成。用于水化学分析的水生生物材料和水样的选择于2022年12月至2023年1月在莫斯科诺金斯克区的EPB RRIFF的池塘中进行,EPB RRIFF是联邦国家预算科学机构FRC RIAH的分支机构,以L.K. Ernst命名。池塘№1的面积为4,48公顷,池塘№2的面积为1,7公顷,池塘№3的面积为0,61公顷。2022年12月至2023年1月进行的研究表明,水质(水化学指标的总和:铵态氮和硝态氮浓度、磷酸盐、总铁、一般硬度和碳酸盐硬度、总矿化以及水温)与底栖无脊椎动物群落(定性和定量)发展指标之间存在相关性。底栖无脊椎动物群落发育指标在水质最好(渔业角度)的池塘中表现最好,在水质最差的池塘中表现最差(该池塘样品中未发现底栖动物)。研究池中共发现底栖无脊椎动物群落6个分类。
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引用次数: 0
Chemical contamination of feed, containing animal raw materials 含动物原料的饲料受到化学污染
Pub Date : 2023-04-01 DOI: 10.30917/att-vk-1814-9588-2023-2-17
K.A. Savenkov, D. A. Makarov, O. I. Lavrukhina, A. V. Tretyakov
Raw feed materials of animal origin intended to produce feed for livestock and “companion animals may contain undesired chemical substances able to be carried-“over in the final produce: toxic elements and veterinary drug residues “(antimicrobials, coccidiostats, antiprotozoal and sedative drugs). The carry-over of “contaminant from raw materials to ready feed is determined by it’s structure and “chemical properties, resistance to environmental conditions and processing. “Elevated levels of toxic elements in the environment are often a result of industrial “and agricultural activities (e.g. use pesticides containing toxic elements, use of “manure as fertilizers and industrial wastewaters), however in some cases “contamination may have natural geological sources. Organic forms of cadmium “and mercury may be more toxic than their inorganic forms due to higher biological “activity. Rise of anthropogenic load leads to t elevated levels of raw feed materials “contamination and susbsequently of feed contamination, posing the risk to animal “health. Literature analysis showed that monitoring of cadmium, lead, mercury and “arsenic should be focused at raw feed materials of mineral and marine origin, “especially at the fishmeal, which contains large amounts of arsenic. The main “source of companion animals feed contamination by antimicrobials are raw “materials, and for livestock cross-contamination is an important factor. Control of “cross-contamination is possible only if good manufacturing practices are applied.“
用于生产牲畜和“伴侣动物”饲料的动物源性原料可能含有不需要的化学物质,可在“最终产品”中携带:有毒元素和兽药残留物”(抗菌剂、抗球药、抗原虫药和镇静剂)。污染物从原料到预备饲料的携带性是由原料的结构和化学性质、对环境条件的耐受性和加工过程决定的。“环境中有毒元素水平升高往往是工业”和农业活动(例如使用含有有毒元素的农药,使用“粪便作为肥料和工业废水”)的结果,但在某些情况下,污染可能有自然地质来源。有机形式的镉和汞可能比无机形式的镉和汞毒性更大,因为它们的生物活性更高。人为负荷的增加导致原料“污染”水平升高,进而导致饲料污染,对动物健康构成风险。文献分析表明,对镉、铅、汞和“砷”的监测应集中在矿物和海洋原料上,特别是对含有大量砷的鱼粉。抗微生物药物污染伴侣动物饲料的主要来源是原料,对家畜交叉污染是一个重要因素。只有应用了良好的生产规范,才有可能控制交叉污染。”
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Veterinaria i kormlenie
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