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Establishment of Animal Infection Model of Spirometra Mansoni and Identification of Spirometra Mansoni by Enzyme-Linked Immunosorbent Assay. 建立曼氏螺旋体动物感染模型并通过酶联免疫吸附试验鉴定曼氏螺旋体
IF 1.8 4区 医学 Q3 INFECTIOUS DISEASES Pub Date : 2025-02-01 Epub Date: 2024-10-25 DOI: 10.1089/vbz.2024.0051
Anqi Luo, Shuyu Chen, Mingye He, Xiaoruo Tan, Zhikang Li, Wei Liu, Yisong Liu

Objective: Spirometra mansoni is a crucial zoonotic parasite. Its larvae are more harmful than adult worms due to their ability to migrate through the host's tissues and organs. Therefore, it is necessary to establish an animal model of spargana for observing pathological changes and exploring diagnostic techniques. Methods: In this study, we infected Kunming mice and cats without any pathogens by feeding sparganum (with the scolex and neck) in order to understand the infection cycle of S. mansoni and explore the preservation host of sparganosis. The infection of S. mansoni was determined by fecal detection and enzyme-linked immunosorbent assay (ELISA). Results: In the model of cats, the eggs of S. mansoni were found in the feces ten days after the infection. The serum-specific IgG antibodies against S. mansoni were positive in experimental groups (mice and cats), and after sixty days, the S. mansoni worms isolated from experimental groups were collected. Conclusion: In conclusion, the experimental results show that mice and cats can be stably infected with S. mansoni through feeding sparganum (with the scolex and neck). The infection method of this study has the potential to establish a practical model for investigating the diagnostic process of S. mansoni, laying the groundwork for application and development. ELISA was used to diagnose mice and cats infected with sparganosis mansoni, providing a case for non-invasive identification of animal sparganosis.

目的:曼氏螺旋体是一种重要的人畜共患寄生虫。由于其幼虫能够在宿主的组织和器官中迁移,因此比成虫危害更大。因此,有必要建立斯巴加那虫的动物模型,以观察病理变化并探索诊断技术。研究方法为了了解曼氏疟原虫的感染周期,探索疟原虫病的保存宿主,本研究通过饲喂疟原虫(带喙和颈部)感染昆明小鼠和猫,无任何病原体。通过粪便检测和酶联免疫吸附试验(ELISA)确定曼氏沙门氏菌的感染情况。结果:在猫的模型中,感染十天后粪便中发现了曼氏沙门氏菌卵。实验组(小鼠和猫)血清中针对曼氏沙门氏菌的特异性 IgG 抗体呈阳性,60 天后,从实验组中收集到分离出的曼氏沙门氏菌虫体。结论总之,实验结果表明,小鼠和猫可以通过喂食匙吻鲟(带螯和颈部)稳定感染曼氏沙门氏菌。本研究的感染方法有可能为研究曼氏沙门氏菌的诊断过程建立一个实用模型,为应用和开发奠定基础。利用酶联免疫吸附法诊断感染曼氏疏螺旋体病的小鼠和猫,为非侵入性鉴定动物疏螺旋体病提供了案例。
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引用次数: 0
Antiparasitic Effects of Niosomal Formulations of Curcumin and Silymarin Against Toxoplasma gondii In Vitro. 姜黄素和水飞蓟素的体外制剂对弓形虫的抗寄生效应
IF 1.8 4区 医学 Q3 INFECTIOUS DISEASES Pub Date : 2025-02-01 Epub Date: 2024-08-20 DOI: 10.1089/vbz.2024.0044
Hiba Riyadh Al-Abodi

Background: Toxoplasmosis is caused by infection with Toxoplasma gondii. No Symptoms in healthy people. Notably, very dangerous symptoms in immunocompromised, or patients with immune diseases. Previous research has shown that the parasite's resistance to drugs continues to emerge and has indicated this resistance as a cause for concern. In this context, researchers have a great responsibility to search for alternative treatments, as well as to develop existing ones. Essentially, this improves the therapeutic efficacy of drugs and prevents the emergence of resistance to them. The present study aims to evaluate antitoxoplasma effects of niosomal loaded curcumin and silymarin and their synergistic effects with clindamycin against T. gondii RH strain in vitro. Materials and Methods: Experiments were conducted on the tachyzoites of T. gondii RH-strain, based on: the free and nieosomal compounds of curcumin and silymarin, in addition to the drug clindamycin. Data were collected to estimate parasite viability during exposure to the therapeutic compounds under study using a special MTT assay ((3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolioum bromide) assay: is a colorimetric assay for measuring cellular growth) kit provided by (Bio Idea Company, Tehran, Iran). Hence, the effect of the therapeutic compounds on the parasite load was studied using the quantitative molecular technique real-time PCR. Results: The results indicate that the combination of N-silymarin and N-curcumin with clindamycin has active synergistic effects against T. gondii leading to complete elimination of the parasite. Data revealed that curcumin and silymarin in both their free and nisomal forms had inhibitory effects on the parasite, and minimal toxic effects on normal cells. Conclusions: The results highlight the successful synergistic effect of clindamycin and the niosomal compounds curcumin and silymarin in completely eradicating the T. gondii RH-strain. This finding contributes positively to the field of safe and effective treatments.

背景:弓形虫病是由弓形虫感染引起的。健康人无症状。值得注意的是,免疫力低下或患有免疫疾病的患者会出现非常危险的症状。以往的研究表明,寄生虫的抗药性不断出现,并指出这种抗药性令人担忧。在这种情况下,研究人员肩负着寻找替代治疗方法和开发现有治疗方法的重大责任。从根本上说,这样做可以提高药物的疗效,防止出现抗药性。本研究旨在评估姜黄素和水飞蓟素的抗弓形虫作用,以及它们与克林霉素在体外对淋球菌 RH 株的协同作用。材料与方法:对 RH 株淋病双球菌的寄生虫进行了实验,除了药物克林霉素外,还使用了姜黄素和水飞蓟素的游离和脂质体化合物。研究人员使用由伊朗德黑兰 Bio Idea 公司提供的 MTT(3-(4,5-二甲基噻唑基-2)-2,5-二苯基四氮唑溴化物)检测试剂盒收集数据,以估计寄生虫在接触研究中的治疗化合物期间的存活率。因此,使用定量分子技术实时 PCR 研究了治疗化合物对寄生虫负荷的影响。结果显示结果表明,N-水飞蓟素和 N-姜黄素与克林霉素的组合对刚地虫具有积极的协同作用,可彻底清除寄生虫。数据显示,姜黄素和水飞蓟素的游离态和异构态对寄生虫都有抑制作用,而对正常细胞的毒性影响极小。结论研究结果表明,克林霉素与姜黄素和水飞蓟素这两种异构体化合物在彻底消灭 RH 型淋病双球菌方面具有成功的协同作用。这一发现为安全有效的治疗方法做出了积极贡献。
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引用次数: 0
Prevalence of Toscana Virus Antibodies in Residents of Bulgaria: A Nationwide Study Following the Pandemic of COVID-19. 保加利亚居民的托斯卡纳病毒抗体流行率:COVID-19 大流行后的全国性研究。
IF 1.8 4区 医学 Q3 INFECTIOUS DISEASES Pub Date : 2025-02-01 Epub Date: 2024-10-10 DOI: 10.1089/vbz.2024.0050
Iva Trifonova, Kim Ngoc, Teodora Gladnishka, Elitsa Panayotova, Evgenia Taseva, Vladislava Ivanova, Iva Vladimirova, Eleonora Kuteva, Iva Christova

Introduction: Toscana virus (TOSV) is an arthropod-borne virus transmitted by sandflies and is mainly found in countries around the Mediterranean basin. In this article, we present the findings of a seroprevalence study on TOSV in Bulgaria. We aim to assess the current epidemiological situation regarding TOSV in the country and raise clinical awareness. Methods: Serum samples (n = 1892) were collected in December 2023. Serological tests were performed using a commercial anti-TOSV ELISA kit. Results: Specific immunoglobulin G (IgG) antibodies were detected in 6.4% (121/1892) of the participants. A significantly higher seropositivity rate was found in the age group over 65 years compared with the age groups 18-29 and 40-64 (11.8% vs. 3.9% vs. 3.4%), as well as in males compared with females (8.0% vs. 5.3%). The seroprevalence rates in districts ranged from 0% to 18.5%. Higher seropositivity was found in the southern and northern regions. Conclusions: The seroprevalence rate of TOSV in Bulgaria, found in this study, is a significant decrease compared with the seropositivity rate of 24.5% reported in the country in 2018. The reasons for this are unknown and could possibly be related to the COVID-19 pandemic and the constantly changing environmental conditions. There is also a possibility that the higher seropositivity detected in 2018 together with the rise in clinical cases reported from endemic countries around that time might have been due to an unrecognized TOSV outbreak taking place in this period. Continued clinical awareness and surveillance are necessary for recognition and management of potential cases of TOSV neuroinfection, especially during summer.

简介托斯卡纳病毒(TOSV)是一种由沙蝇传播的节肢动物病毒,主要存在于地中海盆地周边国家。本文介绍了保加利亚 TOSV 血清流行率研究的结果。我们的目的是评估 TOSV 在保加利亚的流行现状,并提高临床认识。研究方法2023 年 12 月采集了血清样本(n = 1892)。使用商用抗 TOSV ELISA 试剂盒进行血清学检测。结果6.4%的参与者(121/1892)检测到特异性免疫球蛋白 G (IgG) 抗体。与 18-29 岁和 40-64 岁年龄组相比,65 岁以上年龄组的血清阳性率明显更高(11.8% vs. 3.9% vs. 3.4%);与女性相比,男性的血清阳性率也明显更高(8.0% vs. 5.3%)。各地区的血清阳性率从 0% 到 18.5% 不等。南部和北部地区的血清阳性率较高。结论与2018年保加利亚报告的24.5%的血清阳性率相比,本研究发现的保加利亚TOSV血清流行率显著下降。其原因尚不清楚,可能与 COVID-19 大流行和不断变化的环境条件有关。还有一种可能性是,2018 年检测到的较高血清阳性率以及流行国家在这一时期报告的临床病例增加,可能是由于这一时期爆发了一场未被发现的 TOSV 疫情。要识别和处理潜在的 TOSV 神经感染病例,尤其是在夏季,有必要继续进行临床宣传和监测。
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引用次数: 0
The Burden of Acinetobacter baumannii Among Pet Dogs and Cats with Respiratory Illness Outside the Healthcare Facilities: A Possible Public Health Concern. 医疗机构外患呼吸道疾病的宠物猫狗中鲍曼不动杆菌的负担:可能引发的公共卫生问题。
IF 1.8 4区 医学 Q3 INFECTIOUS DISEASES Pub Date : 2025-02-01 Epub Date: 2024-10-15 DOI: 10.1089/vbz.2024.0082
Alaa A Shaker, Ahmed Samir, Hala M Zaher, Khaled A Abdel-Moein

Background: Researchers paid more attention to nosocomial Acinetobacter baumannii in veterinary hospitals worldwide; however, the research scope toward community-acquired A. baumannii infections among animals is largely ignored. Therefore, the current study aimed to investigate the role of diseased dogs and cats suffering from respiratory illness in transmission of community-acquired A. baumannii infection and its public health threat. Materials and methods: Oral swabs were collected from 154 pet animals with respiratory signs, including 80 cats and 74 dogs (outpatient visits). The obtained swabs were cultured on CHROMagar™ MH Orientation media for isolation of A. baumannii, and identification of suspected isolates was conducted via Gram staining, conventional biochemical tests, and molecular detection of the blaOXA-51-like gene. Antimicrobial susceptibility testing of A. baumannii isolates was carried out using the disc diffusion method. Results: Overall, 10 (6.5%) out of 154 diseased pet animals were positive for A. baumannii, where 6 (8.1%) and 4 (5%) dogs and cats were positive, respectively. Multidrug-resistant (MDR) A. baumannii was found in 3.9% of the examined animals. The phylogenetic tree analysis revealed that the obtained sequences from dogs and cats were closely related to human and animal sequences. Conclusion: The occurrence of MDR A. baumannii among dogs and cats suffering from respiratory illness highlights the potential role of pet animals in the dissemination of MDR A. baumannii in the community.

背景:世界各地的研究人员对兽医院中的鲍曼不动杆菌给予了更多关注,但对动物中社区获得性鲍曼不动杆菌感染的研究范围却大多被忽视。因此,本研究旨在调查患有呼吸道疾病的患病猫狗在社区获得性鲍曼不动杆菌感染传播中的作用及其对公共卫生的威胁。材料和方法:从 154 只出现呼吸道症状的宠物身上采集口腔拭子,其中包括 80 只猫和 74 只狗(门诊患者)。获得的拭子在 CHROMagar™ MH Orientation 培养基上进行培养,以分离鲍曼不动杆菌,并通过革兰氏染色、常规生化测试和 blaOXA-51 样基因的分子检测对可疑分离物进行鉴定。采用碟片扩散法对鲍曼尼氏菌分离株进行抗菌药敏感性检测。结果显示总体而言,154 只患病宠物中有 10 只(6.5%)对鲍曼林杆菌呈阳性,其中狗和猫分别有 6 只(8.1%)和 4 只(5%)呈阳性。在3.9%的受检动物中发现了耐多药(MDR)鲍曼林杆菌。系统发生树分析表明,从狗和猫身上获得的序列与人类和动物的序列密切相关。结论在患有呼吸道疾病的狗和猫中出现 MDR 鲍曼尼菌突出表明了宠物在 MDR 鲍曼尼菌在社区传播中的潜在作用。
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引用次数: 0
Bat Viral Shedding: A Review of Seasonal Patterns and Risk Factors. 蝙蝠病毒脱落:季节性模式和危险因素综述。
IF 1.8 4区 医学 Q3 INFECTIOUS DISEASES Pub Date : 2025-01-21 DOI: 10.1089/vbz.2024.0091
Yannan Niu, Clifton D McKee

Background: Bats act as reservoirs for a variety of zoonotic viruses, sometimes leading to spillover into humans and potential risks of global transmission. Viral shedding from bats is an essential prerequisite to bat-to-human viral transmission and understanding the timing and intensity of viral shedding from bats is critical to mitigate spillover risks. However, there are limited investigations on bats' seasonal viral shedding patterns and their related risk factors. We conducted a comprehensive review of longitudinal studies on bat viruses with spillover potential to synthesize patterns of seasonal viral shedding and explore associated risk factors. Methods: We extracted data from 60 reviewed articles and obtained 1085 longitudinal sampling events. We analyzed viral shedding events using entropy values to quantitatively assess whether they occur in a consistent, pulsed pattern in a given season. Results: We found that clear seasonal shedding patterns were common in bats. Eight out of seventeen species-level analyses presented clear seasonal patterns. Viral shedding pulses often coincide with bats' life cycles, especially in weaning and parturition seasons. Juvenile bats with waning maternal antibodies, pregnant bats undergoing immunity changes, and hibernation periods with decreased immune responses could be potential risk factors influencing seasonal shedding patterns. Conclusion: Based on our findings, we recommend future longitudinal studies on bat viruses that combine direct viral testing and serological testing, prioritize longitudinal research following young bats throughout their developmental stages, and broaden the geographical range of longitudinal studies on bat viruses based on current surveillance reports. Our review identified critical periods with heightened viral shedding for some viruses in bat species, which would help promote efforts to minimize spillovers and prevent outbreaks.

背景:蝙蝠是多种人畜共患病毒的宿主,有时会导致病毒外溢到人类身上,并存在全球传播的潜在风险。蝙蝠的病毒脱落是蝙蝠向人类传播病毒的必要先决条件,了解蝙蝠病毒脱落的时间和强度对于减轻溢出风险至关重要。然而,对蝙蝠季节性病毒传播模式及其相关危险因素的调查有限。我们对具有外溢潜力的蝙蝠病毒的纵向研究进行了全面回顾,以综合季节性病毒脱落的模式并探索相关的危险因素。方法:我们从60篇综述文章中提取数据,获得1085个纵向抽样事件。我们使用熵值分析病毒脱落事件,定量评估它们是否在给定季节以一致的脉冲模式发生。结果:我们发现明显的季节性脱落模式在蝙蝠中很常见。在17个物种层面的分析中,有8个呈现出明确的季节模式。病毒脱落的脉冲通常与蝙蝠的生命周期一致,尤其是在断奶和分娩季节。母源抗体减弱的幼蝠、经历免疫变化的怀孕蝙蝠以及免疫反应下降的冬眠期可能是影响季节性脱落模式的潜在危险因素。结论:基于我们的研究结果,我们建议未来对蝙蝠病毒进行纵向研究,将直接病毒检测和血清学检测相结合,优先考虑对年轻蝙蝠进行整个发育阶段的纵向研究,并在现有监测报告的基础上扩大蝙蝠病毒纵向研究的地理范围。我们的综述确定了蝙蝠物种中某些病毒高度脱落的关键时期,这将有助于促进努力尽量减少溢出和防止暴发。
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引用次数: 0
The Compound AT13148 Targeting AKT Suppresses Dengue Virus 2 Replication. 靶向AKT的化合物AT13148抑制登革病毒2型复制
IF 1.8 4区 医学 Q3 INFECTIOUS DISEASES Pub Date : 2025-01-08 DOI: 10.1089/vbz.2024.0069
Yangchao Dong, Chuantao Ye, Peijun Han, Wei Ye, Yuan Wang, Jing Yang, Zhikai Xu, Fanglin Zhang, Yingfeng Lei

Background: Dengue virus (DENV) infection, caused by serotypes DENV 1-4, represents a significant global public health challenge, with no antiviral drugs currently available for treatment. The host Protein kinase B (AKT) signaling pathway is crucial for DENV infection, presenting a potential target for antiviral drug development. Objective: This study aimed to evaluate the antiviral activity of kinase inhibitors that target the AKT pathway, focusing on the compound AT13148. Methods: A mini-screening was conducted to identify kinase inhibitors with antiviral properties against DENV-2. The effects of AT13148 on viral RNA replication and translation were assessed in a dose- and time-dependent manner following DENV-2 entry. The mechanism of action was further investigated by evaluating the impact of AT13148 on AKT kinase activity and phosphorylation status. Results: AT13148 exhibited potent antiviral activity against DENV-2, significantly inhibiting viral RNA replication and translation post-entry. The compound was found to inhibit AKT kinase activity through hyperphosphorylation. Conclusion: The findings indicate that AT13148 effectively targets the AKT pathway, demonstrating potential as an antiviral therapeutic against DENV-2 by interfering with the virus's post-entry processes. Further in vivo studies are warranted to assess the efficacy of AT13148 in controlling DENV infection.

背景:由血清型DENV 1-4引起的登革热病毒(DENV)感染是一项重大的全球公共卫生挑战,目前尚无抗病毒药物可用于治疗。宿主蛋白激酶B (AKT)信号通路对DENV感染至关重要,是抗病毒药物开发的潜在靶点。目的:本研究旨在评价靶向AKT通路的激酶抑制剂的抗病毒活性,重点研究化合物AT13148。方法:对具有DENV-2抗病毒特性的激酶抑制剂进行小型筛选。在DENV-2进入后,以剂量和时间依赖的方式评估AT13148对病毒RNA复制和翻译的影响。通过评估AT13148对AKT激酶活性和磷酸化状态的影响,进一步研究其作用机制。结果:AT13148对DENV-2具有较强的抗病毒活性,显著抑制病毒RNA的复制和翻译。发现该化合物通过过度磷酸化抑制AKT激酶活性。结论:研究结果表明AT13148有效靶向AKT通路,通过干扰DENV-2病毒的进入后过程,显示出抗病毒治疗的潜力。需要进一步的体内研究来评估AT13148控制DENV感染的有效性。
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引用次数: 0
Perspective: Incidence of Clinician-Diagnosed Lyme Disease in Manitoba, Canada 2009-2018. 透视:2009-2018年加拿大马尼托巴省临床诊断的莱姆病发病率
IF 1.8 4区 医学 Q3 INFECTIOUS DISEASES Pub Date : 2025-01-06 DOI: 10.1089/vbz.2024.0088
Richard Rusk, Salima Gasmi, Annie-Claude Bourgeois, Mandy Whitlock, Gilles R Detillieux, Kelly Stimpert, David Buckeridge, Jules K Koffi

Introduction: Lyme disease (LD) surveillance yields useful information to monitor the disease trends and spatial distribution. However, due to several factors, the Manitoba Health surveillance system, as with other systems, could be subject to underreporting. Objectives: To estimate the number and incidence of clinician-diagnosed LD over the study period in Manitoba, describe the epidemiology of clinician-diagnosed LD, and compare the findings with Manitoba Health LD surveillance data during the same period to estimate the extent of underreporting. Methods: A retrospective analysis of administrative health data was performed to calculate the number and incidence of clinician-diagnosed LD from 2009 to 2018 in Manitoba and describe the epidemiological characteristics using the International Classification of Diseases, Tenth and Ninth Revision (ICD-10-CA and ICD-9-CM) codes, and antimicrobial drug prescriptions. Conclusion: Of the 1,629,698 registrants within the Manitoba Health Insurance Registry followed over 10 years, 1658 LD events were identified. Most of the cases occurred from May to July and corresponded to the peak activity of the nymphal stage of the blacklegged tick in the province. LD events presented a bimodal distribution with a peak in children between 5 and 9 years of age for both sexes, and a peak in adults from 65 to 84 and from 50 to 74, respectively, for males and females. We estimated that an average of 165 LD events occurred annually in Manitoba (mean annual incidence of 10.17 per 100,000 population), compared with 30 cases per year reported in the surveillance system; therefore, the LD surveillance yields an important underreporting.

导言:莱姆病监测为监测疾病趋势和空间分布提供了有用的信息。然而,由于若干因素,马尼托巴省卫生监测系统与其他系统一样,可能存在漏报现象。目的:估计马尼托巴省在研究期间临床诊断的LD的数量和发病率,描述临床诊断的LD的流行病学,并将研究结果与马尼托巴省同期的LD监测数据进行比较,以估计漏报的程度。方法:回顾性分析马尼托巴省2009 - 2018年行政卫生数据,计算临床诊断的LD数量和发病率,并使用国际疾病分类第十版和第九版(ICD-10-CA和ICD-9-CM)代码和抗菌药物处方描述流行病学特征。结论:在马尼托巴健康保险登记处随访10年的1,629,698名登记者中,确定了1658例LD事件。病例多发生在5 ~ 7月,与该省黑腿蜱若虫期的活动高峰期相对应。LD事件呈双峰分布,男女均在5 - 9岁儿童中出现高峰,男性和女性分别在65 - 84岁和50 - 74岁的成年人中出现高峰。我们估计马尼托巴省每年平均发生165例LD事件(平均年发病率为每10万人10.17例),而监测系统每年报告的病例为30例;因此,LD监测产生了一个重要的漏报。
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引用次数: 0
Coinfection with Orthohantavirus and Leptospira spp. in Rats Collected from Markets in Indonesia. 印度尼西亚市场上收集的大鼠同时感染正黄病毒和钩端螺旋体的情况
IF 1.8 4区 医学 Q3 INFECTIOUS DISEASES Pub Date : 2025-01-01 Epub Date: 2024-10-18 DOI: 10.1089/vbz.2023.0170
Kozue Miura, James Chambers, Naohiro Takahashi, Harimurti Nuradji, Nlp Indi Dharmayanti, Susanti, Parriantariksina Randusari, Susan M Noor, Rahmat Setya Adji, Muharam Saepulloh, Sumarningsih, Kumiko Yoshimatsu, Nobuo Koizumi

Background: Rats are an important reservoir animal for several zoonotic pathogens worldwide, including hantaviruses and Leptospira spp., which are the causative agents of hemorrhagic fever with renal syndrome, hantavirus cardiopulmonary syndrome, and leptospirosis. Although a previous study indicated a high frequency of antihantaviral antibodies in patients with acute fever in Indonesia, circulating hantaviruses and their reservoir animals in the country remain limited. Materials and Methods: The presence of hantavirus in rats captured in the urban area of Bogor, Indonesia, from which Leptospira spp. were isolated using PCR, followed by DNA sequencing. Immunohistochemical analyses were performed to detect hantaviral and leptospiral antigens in rat kidney tissues. Results: Seoul of Orthohantavirus seoulense (SEOV) RNA was detected from 24 of 80 Rattus norvegicus (30%). SEOV and Leptospira coinfection was detected in 10 of 80 rats (12.5%). Immunohistochemistry revealed that hantavirus antigens were positively stained in the interstitial capillaries and cells, whereas Leptospira antigens were stained in the luminal side of the renal tubules. Conclusion: This study revealed a high prevalence of SEOV and SEOV and Leptospira coinfection among rats in the urban areas of Bogor, Indonesia, indicating a potential risk of rat-borne zoonotic diseases in the area.

背景:大鼠是全球几种人畜共患病病原体的重要贮藏动物,其中包括汉坦病毒和钩端螺旋体属,它们是出血热伴肾综合征、汉坦病毒心肺综合征和钩端螺旋体病的病原体。尽管之前的一项研究表明,印尼急性发热患者体内抗汉坦病毒抗体的频率很高,但该国的循环汉坦病毒及其宿主动物仍然有限。材料和方法:在印度尼西亚茂物市区捕获的老鼠中发现了汉坦病毒,利用聚合酶链式反应(PCR)从老鼠身上分离出钩端螺旋体,然后进行 DNA 测序。通过免疫组化分析检测大鼠肾脏组织中的汉坦病毒和钩端螺旋体抗原。结果80只鼠中有24只(30%)检测到首尔汉坦病毒(SEOV)RNA。在 80 只大鼠中有 10 只(12.5%)检测到 SEOV 和钩端螺旋体合并感染。免疫组化显示,汉坦病毒抗原在肾间质毛细血管和细胞中呈阳性染色,而钩端螺旋体抗原则在肾小管管腔侧染色。结论这项研究揭示了印尼茂物市区老鼠中 SEOV 和 SEOV 与钩端螺旋体混合感染的高流行率,表明该地区存在老鼠传播人畜共患病的潜在风险。
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引用次数: 0
Bartonella Species in Small Mammals in Turkey: Bartonella bilalgolemii sp. nov. Isolated from a Ural Field Mouse (Apodemus uralensis). 土耳其小型哺乳动物中的巴顿氏菌:从乌拉尔田鼠(Apodemus uralensis)身上分离出的 Bartonella bilalgolemii sp.
IF 1.8 4区 医学 Q3 INFECTIOUS DISEASES Pub Date : 2025-01-01 Epub Date: 2024-10-31 DOI: 10.1089/vbz.2024.0026
Bekir Çelebi, Rita Zgheib, Ahmet Karataş, Cahit Babür, İbrahim Mehmet Ali Öktem, Ferhat Matur, Mustafa Sözen, Bernard Davoust, Oleg Mediannikov, Pierre-Edouard Fournier

Background: The genus Bartonella is composed of Gram-negative, fastidious, facultative intracellular bacteria that can cause bacteremia in mammals and various disorders in humans. Rodents have been reported as reservoirs of more than 30 Bartonella species, seven of which cause zoonotic infections. Materials and Methods: In the present study, the isolation of Bartonella sp. was attempted from 150 spleen samples from 13 rodent species (mostly Apodemus species) from three geographically different regions in Turkey. Results: Bartonella sp. was successfully isolated from 65 of these 150 samples (43%). The prevalences of Bartonella sp. in tested rodents in the regions of Giresun, Yozgat, and Burdur were 68%, 44%, and 16%, respectively. Using polymerase chain reaction/sequence analysis of the citrate synthase-coding gene (gltA), Bartonellaisolates were classified seven species including B. taylorii, B. grahamii, B. birtlesii, B. mastomydis, and three putatively new Bartonella species. We performed further identification techniques for one of the three Bartonella species that were different from the validated Bartonella species according to the gltA sequence analysis. Conclusion: Here, we report the genomic and phenotypic characterization of Bartonella sp. strain G70 that was isolated from the splenic tissue of an Apodemus uralensis (Pallas 1881), the Ural field mouse, captured in the Giresun region of northeastern Turkey. Bartonella sp. strainG70 (RSKK 22001) was characterized by whole genome and partial gene (gltA, 16S ribosomal RNA) sequencing and comparison, scanning electron microscopy, biochemical tests, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. This novel Bartonella is a Gram-negative, rod-shaped bacterium and has neither flagella nor pili. The genome from strain G70 was 1,606,969-bp-long with a G + C content of 35.7%. Bartonella rochalimae was found to be the closest phylogenetic relative of strain G70 (OrthoANI = 90.5%, digital DNA-DNA hybridization = 41.4%). We therefore propose that this new species be named Bartonella bilalgolemii sp. nov. with strain G70T as the type strain.

背景:巴顿氏菌属由革兰氏阴性、繁殖快、细胞内兼性细菌组成,可导致哺乳动物菌血症和人类各种疾病。据报道,啮齿动物是 30 多种巴顿氏菌的贮藏地,其中 7 种会引起人畜共患病。材料和方法:本研究尝试从土耳其三个不同地区 13 种啮齿动物(主要是 Apodemus 种)的 150 份脾脏样本中分离巴顿氏菌。结果:在这 150 份样本中,有 65 份(43%)成功分离出巴顿氏杆菌。吉雷松、约兹加特和布尔杜尔地区受检啮齿动物的巴顿氏菌感染率分别为 68%、44% 和 16%。通过对柠檬酸合成酶编码基因(gltA)进行聚合酶链反应/序列分析,巴顿菌分离物被分为 7 个种,包括泰勒氏巴顿菌(B. taylorii)、格雷厄姆氏巴顿菌(B. grahamii)、伯特氏巴顿菌(B. birtlesii)、马斯托米迪氏巴顿菌(B. mastomydis)和 3 个推测的巴顿菌新种。根据 gltA 序列分析,我们对这三个巴顿菌种中的一个进行了进一步的鉴定。结论在此,我们报告了从土耳其东北部吉雷松地区捕获的乌拉尔田鼠 Apodemus uralensis (Pallas 1881) 脾脏组织中分离出的巴顿菌 G70 株的基因组和表型特征。通过全基因组和部分基因(gltA、16S 核糖体 RNA)测序和比较、扫描电子显微镜、生化测试和基质辅助激光解吸电离飞行时间质谱分析,对巴顿氏菌 G70 株(RSKK 22001)进行了鉴定。这种新型巴顿氏菌是一种革兰氏阴性、杆状细菌,既无鞭毛也无纤毛。菌株 G70 的基因组长 1,606,969 bp,G+C 含量为 35.7%。研究发现,罗沙利玛氏巴顿菌是 G70 菌株在系统发育上的近亲(OrthoANI = 90.5%,数字 DNA-DNA 杂交 = 41.4%)。因此,我们建议将这一新种命名为 Bartonella bilalgolemii sp.
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引用次数: 0
Brucellosis Coinfecting with Liver Hydatid Cyst: Case Report and Literature Review of Zoonoses Coinfection of Human Brucellosis. 布鲁氏菌病合并肝包虫囊肿:人畜共患病合并感染人类布鲁氏菌病的病例报告和文献综述。
IF 1.8 4区 医学 Q3 INFECTIOUS DISEASES Pub Date : 2025-01-01 Epub Date: 2024-10-18 DOI: 10.1089/vbz.2024.0062
Dai Peijun, Shen Weiwei, Pu Zhongshu

Background: Brucellosis is a major global public health problem. Brucellosis usually coinfects with zoonoses because of their similar reservoir and infection routes. Materials and Methods: Here, we present a patient who was diagnosed with coinfecting with liver cyst and brucellosis. Results: A 57-year-old female farmer was hospitalized with right hip joint pain approximately 4 weeks earlier. The patient reported a history of hydatid cysts and a family history of brucellosis. Abdominal computerized tomography and ultrasonography revealed a liver hydatid cyst. The standard agglutination test titer of Wright's test was 1:100. Computerized tomography and magnetic resonance imaging revealed brucellosis hip arthritis. The patient was treated with a combination of doxycycline, rifampicin, and cefotaxime. The symptoms immediately improved. The patient was well and asymptomatic during the 6-month follow-up. We reviewed the literature on other zoonoses superinfection with brucellosis. Conclusion: Brucellosis coinfects with multiple zoonosis organisms and poses a serious health threat to humans. Awareness of possible brucellosis coinfection with other zoonoses, especially professionally exposed persons in endemic regions, is warranted.

背景:布鲁氏菌病是一个重大的全球公共卫生问题。布鲁氏菌病通常与人畜共患疾病同时感染,因为它们有相似的储库和感染途径。材料与方法:本文介绍了一名被诊断为肝囊肿和布鲁氏菌病并发感染的患者。结果:一名 57 岁的女性农民约 4 周前因右髋关节疼痛住院。患者称有包虫囊肿病史和布鲁氏菌病家族史。腹部计算机断层扫描和超声波检查发现了肝包虫囊肿。赖特氏试验的标准凝集试验滴度为 1:100。计算机断层扫描和磁共振成像显示患者患有布鲁氏菌病髋关节炎。患者接受了强力霉素、利福平和头孢他啶的联合治疗。症状立即得到改善。在 6 个月的随访期间,患者状况良好,无任何症状。我们查阅了有关其他人畜共患病超级感染布鲁氏菌病的文献。结论是布鲁氏菌病与多种人畜共患病同时感染,对人类健康构成严重威胁。需要警惕布鲁氏菌病可能与其他人畜共患病,特别是流行地区的职业接触者。
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Vector borne and zoonotic diseases
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