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19 Mikroorganismen als Symbionten und Antagonisten 19微生物是共生和对立主义者
Pub Date : 2022-01-01 DOI: 10.1055/b-0041-183810
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引用次数: 0
7 Wachstum und Ernährung der Mikroorganismen 7微生物的生长和营养
Pub Date : 2022-01-01 DOI: 10.1055/B-0037-144641
G. Fuchs
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引用次数: 0
1 Die Mikroorganismen – eine kurze Einführung 1微生物学——初步的介绍
Pub Date : 2022-01-01 DOI: 10.1055/b-0037-144632
G. Fuchs
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引用次数: 0
10 Transport durch die Cytoplasmamembran
Pub Date : 2022-01-01 DOI: 10.1055/b-0037-144646
G. Fuchs
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引用次数: 0
20 Mikroorganismen im Dienste des Menschen: Biotechnologie 20微生物为人类服务:生物技术
Pub Date : 2022-01-01 DOI: 10.1055/B-0037-144661
G. Fuchs
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引用次数: 0
12 Oxidation anorganischer Verbindungen: chemolithotrophe Lebensweise 12无生命化学药剂氧化
Pub Date : 2022-01-01 DOI: 10.1055/b-0037-144648
G. Fuchs
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引用次数: 0
17 Mikrobielle Vielfalt, Evolution und Systematik "微生物多样性,进化和种类史系统学
Pub Date : 2022-01-01 DOI: 10.1055/b-0041-183808
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引用次数: 0
Protein patterns of Streptomyces griseus strains during development. 灰色链霉菌菌株在发育过程中的蛋白质模式。
Pub Date : 1984-01-01 DOI: 10.1002/jobm.3630240315
S Vitális, I Békési, G Valu, G Szabó

Protein patterns from mycelial extracts of Streptomyces griseus strains No. 45H and No. 52-1 were studied by one dimensional gradient PAGE with 20 cm run. The results are reproducible, the protein patterns are the same for the same developmental stage in a given strain. There are well-defined characteristic changes of the protein patterns during the life cycle. The proteins of spores show conspicuous differences compared to protein patterns of the old (72 h) mycelia in the same culture. There is no difference between protein patterns of spores from submerged and from solid media. The protein patterns of two closely related Streptomyces griseus strains significantly differ from each other. After pulse labelling with (14C)-labelled protein hydrolyzate for 40 min, the specific activities of the proteins show considerable differences. There are characteristic bands with low specific activity and others with high incorporation. The fluorograms of the 16, 40 and 64 h cultures show that different proteins are being synthesized intensively at different ages of the culture.

采用一维梯度PAGE技术研究了灰色链霉菌45H和52-1菌株菌丝体提取物的蛋白图谱。结果是可重复的,在同一菌株的相同发育阶段,蛋白质模式是相同的。在生命周期中,蛋白质模式有明确的特征变化。在相同的培养条件下,孢子的蛋白质与老菌丝(72 h)的蛋白质模式有显著差异。浸没培养基和固体培养基中孢子的蛋白质形态没有差别。两株亲缘关系较近的灰色链霉菌的蛋白谱差异显著。用(14C)标记的蛋白水解物脉冲标记40分钟后,蛋白质的比活性显示出相当大的差异。有低比活性的特征带和高结合的特征带。16h、40h和64h培养的荧光图显示,在不同的培养年龄,不同的蛋白质正在密集合成。
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引用次数: 0
Nuclear inheritance of the biosynthesis of cyclopenin and cyclopenol in Penicillium cyclopium. 环青霉环青霉素和环酚生物合成的核遗传。
Pub Date : 1984-01-01 DOI: 10.1002/jobm.3630240908
Z S Mohamed, R W Todorova-Draganova, M Luckner

Balanced heterokarions were grown from Penicillium cyclopium aux-glu 1, a glutamic acid auxotroph producing benzodiazepine alkaloids of the cyclopenin-cyclopenol group, and P. viridicatum aux-met 1, a methionine auxotroph forming these alkaloids in traces only. In contrast to the hyphae of the parent strains, the hyphae of the heterokarions were dark orange-brown and grew well on media without the auxotrophic factors. In surface cultures they synthesized the benzodiazepine alkaloids cyclopenin and cyclopenol in amounts similar to those formed by the hyphae of P. cyclopium aux-glu 1. From the monokariotic conidiospores of the heterokarions homokariotic daughter strains were obtained which were similar to the parent strains in every respect. Hence no exchange of features of cyclopenin-cyclopenol biosynthesis took place between the parent strains at the stage of the heterokarion. This result indicates that the formation of cyclopenin and cyclopenol in P. cyclopium aux-glu 1 and the nearly complete lack of biosynthesis of these compounds in P. viridicatum aux-met 1 is encoded within the nucleus and is not influenced by plasmic genetic material.

从产生环青霉素-环苯酚类苯二氮卓类生物碱的谷氨酸营养不良菌青霉cyclopium aux-glu 1和产生这些生物碱的蛋氨酸营养不良菌P. viglomeratum aux-met 1中培养出平衡的异离子型。与亲本菌株的菌丝相比,异基因菌丝呈深橙褐色,在不添加营养不良因子的培养基上生长良好。在表面培养中,他们合成了苯二氮卓类生物碱环penin和环penol,其量与P. cyclopium aux-glu 1菌丝形成的量相似。从异核子代的单核分生孢子中获得了与亲本各方面相似的异核子代。因此,在异离子化阶段,亲本菌株之间没有环萜素-环萜醇生物合成特征的交换。这一结果表明,环萜素和环萜醇在P. cyclopium aux-glu 1中的形成以及这些化合物在P. vipumatum aux-met 1中几乎完全缺乏生物合成是在细胞核内编码的,不受质遗传物质的影响。
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引用次数: 0
Occurrence of gentamicin-producing micromonosporae in Lake Balaton (W. Hungary). 匈牙利西部巴拉顿湖产庆大霉素小单孢子菌的发生。
Pub Date : 1984-01-01 DOI: 10.1002/jobm.3630240915
Z Szabó

The total number of platable bacteria in the wet mud material characterized by the predominant occurrence of high-magnesian calcite and protodolomite of the Lake Balaton fluctuates between 3.5 X 10(4) and 2.3 X 10(5) g-1. Actinomycetes constitute about 25-30 per cent of this mud-community and not more than half of them belong to the genus Micromonospora. The presence of 10 species of this genus was detected among the isolated and studied strains identified by means of conventional methods and computer aided numerical analysis in which 20 authentic, mostly type strains of Micromonospora spp. were also involved. All of the three species of gentamicin-producing Micromonospora (M. echinospora, M. purpurea and M. sagamiensis) known so far were found in mud samples. These three species form stable but less numerous populations (less than 0.5 per cent of the total platable microflora) in the Balaton's mud, the members of which are capable to produce gentamicins at about 40-50 micrograms ml-1 level at most.

巴拉顿湖以高镁方解石和原白云石为主的湿泥物质中可平板细菌总数在3.5 × 10(4) ~ 2.3 × 10(5) g-1之间波动。放线菌约占这一淤泥群落的25- 30%,其中不超过一半属于小单孢子菌属。通过常规方法和计算机辅助数值分析鉴定的分离和研究菌株中检出该属10种,其中包括20株真实的、以型为主的小单孢子菌。目前已知的3种产庆大霉素的小单孢菌(棘孢菌、紫孢菌和sagamiensis)均在泥样中发现。这三个物种在巴拉顿的泥浆中形成稳定但数量较少的种群(不到总平板微生物群的0.5%),其中的成员最多能够产生约40-50微克ml-1的庆大霉素。
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引用次数: 0
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