I Zehbe, M Schmidt, M Maeurer, C Leo, M Höckel, H Pilch
Objective: Cervical cancer is associated with infection of epithelial cells with the human papillomavirus (HPV) type 16 and HPV18. A functional signalling machinery in T-cells is required in order to successfully fight and eradicate HPV16+ transformed epithelial cells. One of the key signalling molecules associated with the T-cell receptor (TCR) is the homodimeric zeta chain molecule.
Material and methods: 28 formalin fixed und paraffin embedded samples of cervical tissue with cervical intraepithelial lesions CIN I (n = 3), CIN III (n = 7), invasive cervical carcinoma (CC) (n = 13) and normal cervical tissue (n = 5) has been evaluated for HPV-PCR und zeta chain immunohistochemistry. For immunohistochemistry a monoclonal IgG1 anti TZR zeta chain-antibody (mAb) has been used (clone 6B 10.2, Santa Cruz, Heidelberg, Germany). According to the performed Western-Blot analysis on peripheral blood monocytes (PBMCs) the used mAb has specifically recognized TCR zeta chains.
Results: We show reduced protein zeta chain expression associated with invasive cervical cancer, but not with pre-invasive HPV16-positive lesions or HPV16-negative normal cervix tissue.
Conclusions: Thus, reduced TCR zeta chain expression is not necessarily linked to a chronic viral infection, nor to the presence of transformed cells, but rather to the stromal invasion of the cancer lesion.
目的:宫颈癌与人乳头瘤病毒(HPV) 16型和HPV18型上皮细胞感染有关。为了成功地对抗和根除HPV16+转化的上皮细胞，t细胞中的功能信号机制是必需的。与t细胞受体(TCR)相关的关键信号分子之一是二聚体zeta链分子。材料与方法:对宫颈上皮内病变CIN I (n = 3)、CIN III (n = 7)、浸润性宫颈癌(n = 13)和正常宫颈组织(n = 5) 28例宫颈组织进行HPV-PCR和zeta链免疫组织化学检测。免疫组化使用单克隆IgG1抗TZR zeta链抗体(mAb)(克隆6B 10.2, Santa Cruz, Heidelberg，德国)。根据对外周血单核细胞(PBMCs)进行的Western-Blot分析，所使用的单抗特异性识别TCR zeta链。结果:我们发现zeta链蛋白表达减少与侵袭性宫颈癌有关，但与侵袭前hpv16阳性病变或hpv16阴性正常宫颈组织无关。结论:因此，TCR zeta链表达的减少不一定与慢性病毒感染有关，也不一定与转化细胞的存在有关，而是与癌病变的间质浸润有关。
{"title":"Different T-cell receptor (TCR) zeta chain expression in cervical cancer and its precursor lesions.","authors":"I Zehbe, M Schmidt, M Maeurer, C Leo, M Höckel, H Pilch","doi":"10.1055/s-2006-933423","DOIUrl":"https://doi.org/10.1055/s-2006-933423","url":null,"abstract":"<p><strong>Objective: </strong>Cervical cancer is associated with infection of epithelial cells with the human papillomavirus (HPV) type 16 and HPV18. A functional signalling machinery in T-cells is required in order to successfully fight and eradicate HPV16+ transformed epithelial cells. One of the key signalling molecules associated with the T-cell receptor (TCR) is the homodimeric zeta chain molecule.</p><p><strong>Material and methods: </strong>28 formalin fixed und paraffin embedded samples of cervical tissue with cervical intraepithelial lesions CIN I (n = 3), CIN III (n = 7), invasive cervical carcinoma (CC) (n = 13) and normal cervical tissue (n = 5) has been evaluated for HPV-PCR und zeta chain immunohistochemistry. For immunohistochemistry a monoclonal IgG1 anti TZR zeta chain-antibody (mAb) has been used (clone 6B 10.2, Santa Cruz, Heidelberg, Germany). According to the performed Western-Blot analysis on peripheral blood monocytes (PBMCs) the used mAb has specifically recognized TCR zeta chains.</p><p><strong>Results: </strong>We show reduced protein zeta chain expression associated with invasive cervical cancer, but not with pre-invasive HPV16-positive lesions or HPV16-negative normal cervix tissue.</p><p><strong>Conclusions: </strong>Thus, reduced TCR zeta chain expression is not necessarily linked to a chronic viral infection, nor to the presence of transformed cells, but rather to the stromal invasion of the cancer lesion.</p>","PeriodicalId":23881,"journal":{"name":"Zentralblatt fur Gynakologie","volume":"128 5","pages":"266-70"},"PeriodicalIF":0.0,"publicationDate":"2006-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-2006-933423","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26331375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Audiovisuelle Narkoseaufklärung in der Geburtshilfe","authors":"T. Laux, C. Madler","doi":"10.1055/S-2006-950550","DOIUrl":"https://doi.org/10.1055/S-2006-950550","url":null,"abstract":"","PeriodicalId":23881,"journal":{"name":"Zentralblatt fur Gynakologie","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2006-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80147193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Wo sind die Fortschritte in der gynäkologischen Onkologie","authors":"M. Kaufmann","doi":"10.1055/S-2006-950567","DOIUrl":"https://doi.org/10.1055/S-2006-950567","url":null,"abstract":"","PeriodicalId":23881,"journal":{"name":"Zentralblatt fur Gynakologie","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2006-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80236554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Puhl, C. Interthal, C. Lindner, V. Passuello, D. Macchiella, E. Steiner, H. Kölbl
{"title":"Der Einfluss maternalen Alters auf die Assoziation eines isolierten fetalen intrakardialen echogenen Fokus mit einer chromosomalen Aberration","authors":"A. Puhl, C. Interthal, C. Lindner, V. Passuello, D. Macchiella, E. Steiner, H. Kölbl","doi":"10.1055/S-2006-950544","DOIUrl":"https://doi.org/10.1055/S-2006-950544","url":null,"abstract":"","PeriodicalId":23881,"journal":{"name":"Zentralblatt fur Gynakologie","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2006-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85596219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Rody, U. Holtrich, C. Solbach, V. Müller, E. Ruckhäberle, R. Gätje, M. Gehrmann, G. Minckwitz, T. Karn, M. Kaufmann
{"title":"Das Mammastammzellkonzept – ein theoretisches Konstrukt oder klinisch bedeutsam?","authors":"A. Rody, U. Holtrich, C. Solbach, V. Müller, E. Ruckhäberle, R. Gätje, M. Gehrmann, G. Minckwitz, T. Karn, M. Kaufmann","doi":"10.1055/S-2006-950561","DOIUrl":"https://doi.org/10.1055/S-2006-950561","url":null,"abstract":"","PeriodicalId":23881,"journal":{"name":"Zentralblatt fur Gynakologie","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2006-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76366780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Wieviel Zurückhaltung ist geboten beim Einsatz transvaginaler Netze? Überlegungen zur Indikationsstellung innovativer Methoden","authors":"W. Adelhardt","doi":"10.1055/S-2006-950519","DOIUrl":"https://doi.org/10.1055/S-2006-950519","url":null,"abstract":"","PeriodicalId":23881,"journal":{"name":"Zentralblatt fur Gynakologie","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2006-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83606559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Audiovisuelle Narkoseaufklärung in der operativen Gynäkologie","authors":"T. Laux, C. Madler","doi":"10.1055/S-2006-950569","DOIUrl":"https://doi.org/10.1055/S-2006-950569","url":null,"abstract":"","PeriodicalId":23881,"journal":{"name":"Zentralblatt fur Gynakologie","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2006-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85785600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Melchert, L. Bauer, U. Kraus-Tiefenbacher, F. Wenz
{"title":"Intraoperative Strahlentherapie des Mammakarzinoms – Derzeitiger Stellenwert","authors":"F. Melchert, L. Bauer, U. Kraus-Tiefenbacher, F. Wenz","doi":"10.1055/S-2006-950564","DOIUrl":"https://doi.org/10.1055/S-2006-950564","url":null,"abstract":"","PeriodicalId":23881,"journal":{"name":"Zentralblatt fur Gynakologie","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2006-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79936589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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