An experiment was conducted to investigate the effect of cobalt supplementation on hair follicle development in rabbits. Rex rabbits (30-d-old, n=180) were divided randomly into five equal treatment groups: rabbits fed a basal diet (control, measured cobalt content of 0.27 mg/kg) or rabbits fed a basal diet with an additional 0.1, 0.4, 1.6 or 6.4 mg/kg cobalt (in the form of cobalt sulfate) supplementation (measured cobalt contents of 0.35, 0.60, 1.83 and 6.62 mg/kg, respectively). Treatment with 6.4 mg/kg cobalt significantly decreased hair follicle density ( P 0.05). The addition of dietary cobalt at the highest level examined (6.4 mg/kg) significantly increased the gene expression of bone morphogenetic protein (BMP) 2 and BMP4 in skin tissue ( P 0.05). Compared with their levels in the control group, dietary cobalt treatment significantly suppressed the protein levels of p-mechanistic target of rapamycin (mTOR) and p-ribosomal protein S6 protein kinase ( P 0.05). In conclusion, cobalt at the highest concentration examined inhibited hair follicle development, which may have involved the mTOR-BMP signalling pathway.
{"title":"High doses of cobalt inhibited hair follicle development in Rex Rabbits","authors":"L. Liu, Q. Gao, C. Wang, Z. Fu, K. Wang, F. Li","doi":"10.4995/wrs.2019.12038","DOIUrl":"https://doi.org/10.4995/wrs.2019.12038","url":null,"abstract":"An experiment was conducted to investigate the effect of cobalt supplementation on hair follicle development in rabbits. Rex rabbits (30-d-old, n=180) were divided randomly into five equal treatment groups: rabbits fed a basal diet (control, measured cobalt content of 0.27 mg/kg) or rabbits fed a basal diet with an additional 0.1, 0.4, 1.6 or 6.4 mg/kg cobalt (in the form of cobalt sulfate) supplementation (measured cobalt contents of 0.35, 0.60, 1.83 and 6.62 mg/kg, respectively). Treatment with 6.4 mg/kg cobalt significantly decreased hair follicle density ( P 0.05). The addition of dietary cobalt at the highest level examined (6.4 mg/kg) significantly increased the gene expression of bone morphogenetic protein (BMP) 2 and BMP4 in skin tissue ( P 0.05). Compared with their levels in the control group, dietary cobalt treatment significantly suppressed the protein levels of p-mechanistic target of rapamycin (mTOR) and p-ribosomal protein S6 protein kinase ( P 0.05). In conclusion, cobalt at the highest concentration examined inhibited hair follicle development, which may have involved the mTOR-BMP signalling pathway.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":"27 1","pages":"217-225"},"PeriodicalIF":0.8,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46282107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of this study was to investigate the effects of two sublethal doses of gossypol (GOS) (4 and 20 mg/kg of body weight), administered every other day, on some haematological, biochemical, enzymatic and electrolytic properties and amino and fatty acids in male rabbit blood plasma. The experiment lasted for 16 wk and included two phases: 1) administration period; rabbits were given the experimental doses of GOS for 8 wk; and 2) recovery period; rabbits were allowed 8 wk for complete withdrawal of drugs from the plasma. Results showed that low levels of gossypol increased ( P <0.01) haemoglobin, mean corpuscular haemoglobin and white blood cells compared to control. Plasma total protein was increased ( P <0.01) by the low GOS dose in both experimental phases. Likewise, glucose concentration was increased ( P <0.01) by the high GOS dose during the recovery period. Aspartate aminotransferase and alanine aminotransferase enzymes were increased ( P <0.01) by the high dose of GOS treatment only. Low GOS dose increased ( P <0.01) blood plasma Na+ concentration in the recovery period only. Results revealed that total essential amino acids (EAA), and EAA/non-EAA ratio were not affected in a dose-dependent manner during the treatment phase expect for plasma proline, which was increased along with non-EAA ( P <0.01) by high GOS dose. Additionally, GOS administration did not affect total unsaturated fatty acids (USFA), total saturated fatty acids (SFA) and SFA/USFA ratio in a dose-dependent manner. In conclusion, Gossypol treatment affected rabbit haematological parameters and biochemical properties of blood plasma in a dose-dependent manner.
{"title":"Effects of sublethal doses of gossypol on haematological properties and biochemical metabolites of male rabbit","authors":"T. Ramadan, A. Rashad","doi":"10.4995/wrs.2019.11253","DOIUrl":"https://doi.org/10.4995/wrs.2019.11253","url":null,"abstract":"The purpose of this study was to investigate the effects of two sublethal doses of gossypol (GOS) (4 and 20 mg/kg of body weight), administered every other day, on some haematological, biochemical, enzymatic and electrolytic properties and amino and fatty acids in male rabbit blood plasma. The experiment lasted for 16 wk and included two phases: 1) administration period; rabbits were given the experimental doses of GOS for 8 wk; and 2) recovery period; rabbits were allowed 8 wk for complete withdrawal of drugs from the plasma. Results showed that low levels of gossypol increased ( P <0.01) haemoglobin, mean corpuscular haemoglobin and white blood cells compared to control. Plasma total protein was increased ( P <0.01) by the low GOS dose in both experimental phases. Likewise, glucose concentration was increased ( P <0.01) by the high GOS dose during the recovery period. Aspartate aminotransferase and alanine aminotransferase enzymes were increased ( P <0.01) by the high dose of GOS treatment only. Low GOS dose increased ( P <0.01) blood plasma Na+ concentration in the recovery period only. Results revealed that total essential amino acids (EAA), and EAA/non-EAA ratio were not affected in a dose-dependent manner during the treatment phase expect for plasma proline, which was increased along with non-EAA ( P <0.01) by high GOS dose. Additionally, GOS administration did not affect total unsaturated fatty acids (USFA), total saturated fatty acids (SFA) and SFA/USFA ratio in a dose-dependent manner. In conclusion, Gossypol treatment affected rabbit haematological parameters and biochemical properties of blood plasma in a dose-dependent manner.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":"27 1","pages":"237-248"},"PeriodicalIF":0.8,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44777380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of this study was to add to the current understanding of rabbit caecal microbiota. This involved describing its microbial composition and linking this to live performance parameters, as well as determining the effects of dietary quercetin (Qrc) supplementation (2 g/kg feed) and sex on the microbial population. The weight gain and feed conversion ratio of twelve New Zealand White rabbits was measured from 5 to 12 wk old, blood was sampled at 11 wk old for the determination of serum hormone levels, and the rabbits were slaughtered and caecal samples collected at 13 wk old. Ion 16STM metagenome sequencing was used to determine the microbiota profile. The dominance of Firmicutes (72.01±1.14% of mapped reads), Lachnospiraceae (23.94±1.01%) and Ruminococcaceae (19.71±1.07%) concurred with previous reports, but variation both between studies and individual rabbits was apparent beyond this. Significant correlations between microbial families and live performance parameters were found, suggesting that further research into the mechanisms of these associations could be useful. Negative correlations with the caecal flavonoid content were found, but the latter was not affected by diet, and the effects of quercetin supplementation on the microbiota were very limited, possibly due to the absorption of the quercetin-aglycone from the gastrointestinal tract prior to the caecum. Nonetheless, Clostridiales Family XIII. Incertae Sedis was more abundant in the quercetin-supplemented rabbits (Control: 0.003±0.003%; Qrc: 0.020±0.000; P=0.005), as was the genus Anaerofustis (Control: 0.000±0.002; Qrc: 0.010±0.002; P=0.003). Serum cortisol levels were higher in females, and several microbial families differed between the sexes. Most were more abundant in female rabbits, including the most abundant, the family Eubacteriaceae (Male: 2.93±0.40; Female: 4.73±0.40; P=0.01).
{"title":"Composition of rabbit caecal microbiota and the effects of dietary quercetin supplementation and sex thereupon","authors":"M. K. North, A. Dalle Zotte, L. Hoffman","doi":"10.4995/wrs.2019.11905","DOIUrl":"https://doi.org/10.4995/wrs.2019.11905","url":null,"abstract":"The purpose of this study was to add to the current understanding of rabbit caecal microbiota. This involved describing its microbial composition and linking this to live performance parameters, as well as determining the effects of dietary quercetin (Qrc) supplementation (2 g/kg feed) and sex on the microbial population. The weight gain and feed conversion ratio of twelve New Zealand White rabbits was measured from 5 to 12 wk old, blood was sampled at 11 wk old for the determination of serum hormone levels, and the rabbits were slaughtered and caecal samples collected at 13 wk old. Ion 16STM metagenome sequencing was used to determine the microbiota profile. The dominance of Firmicutes (72.01±1.14% of mapped reads), Lachnospiraceae (23.94±1.01%) and Ruminococcaceae (19.71±1.07%) concurred with previous reports, but variation both between studies and individual rabbits was apparent beyond this. Significant correlations between microbial families and live performance parameters were found, suggesting that further research into the mechanisms of these associations could be useful. Negative correlations with the caecal flavonoid content were found, but the latter was not affected by diet, and the effects of quercetin supplementation on the microbiota were very limited, possibly due to the absorption of the quercetin-aglycone from the gastrointestinal tract prior to the caecum. Nonetheless, Clostridiales Family XIII. Incertae Sedis was more abundant in the quercetin-supplemented rabbits (Control: 0.003±0.003%; Qrc: 0.020±0.000; P=0.005), as was the genus Anaerofustis (Control: 0.000±0.002; Qrc: 0.010±0.002; P=0.003). Serum cortisol levels were higher in females, and several microbial families differed between the sexes. Most were more abundant in female rabbits, including the most abundant, the family Eubacteriaceae (Male: 2.93±0.40; Female: 4.73±0.40; P=0.01).","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46088968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carmen Escribá-Pérez, Amparo Baviera-Puig, Luis Montero-Vicente, Juan Buitrago-Vera
The nutritional and dietary properties of rabbit meat make it an ideal food for children, recommended by the World Health Organisation. However, the presence of children under 18 in the home has been found to decrease the frequency of rabbit meat consumption. If we focus on households with children under 18, 52.5% of minors do not consume rabbit meat. The main reason why children (intended as people under 18 yr old) do not consume rabbit meat is the fact that they do not like it (40.9%) and because it is not bought/eaten at home (30.9%). Faced with this situation, there is a pressing need to seek appropriate strategies to adapt rabbit meat for consumption by the youngest family members. In light of the results, the following strategies are proposed. First, the development of functional foods for babies and children, such as rabbit meat enriched with ω3 and docosahexaenoic acid. Secondly, improving meat tenderness. Third, adapting rabbit meat presentations for children (burgers, nuggets, sausages, marinades …), converting them into convenience products for parents and extending their shelf life. Fourth, adapting the labelling/packaging for children to attract attention of both parents and offspring. Finally, developing communication strategies on the nutritional value of rabbit meat aimed at both children and parents. It is observed that if minors consume rabbit meat, they also eat other types of meat such as lamb and beef more often. Therefore, in this type of households a varied and complete diet is consumed in terms of meat consumption, so it would be necessary to rethink joint communication strategies among the three meat sectors. Promoting rabbit meat consumption among the under 18s has several consequences, as in the future they will be in charge of household purchases or share this responsibility.
{"title":"Children's consumption of rabbit meat","authors":"Carmen Escribá-Pérez, Amparo Baviera-Puig, Luis Montero-Vicente, Juan Buitrago-Vera","doi":"10.4995/wrs.2019.11991","DOIUrl":"https://doi.org/10.4995/wrs.2019.11991","url":null,"abstract":"The nutritional and dietary properties of rabbit meat make it an ideal food for children, recommended by the World Health Organisation. However, the presence of children under 18 in the home has been found to decrease the frequency of rabbit meat consumption. If we focus on households with children under 18, 52.5% of minors do not consume rabbit meat. The main reason why children (intended as people under 18 yr old) do not consume rabbit meat is the fact that they do not like it (40.9%) and because it is not bought/eaten at home (30.9%). Faced with this situation, there is a pressing need to seek appropriate strategies to adapt rabbit meat for consumption by the youngest family members. In light of the results, the following strategies are proposed. First, the development of functional foods for babies and children, such as rabbit meat enriched with ω3 and docosahexaenoic acid. Secondly, improving meat tenderness. Third, adapting rabbit meat presentations for children (burgers, nuggets, sausages, marinades …), converting them into convenience products for parents and extending their shelf life. Fourth, adapting the labelling/packaging for children to attract attention of both parents and offspring. Finally, developing communication strategies on the nutritional value of rabbit meat aimed at both children and parents. It is observed that if minors consume rabbit meat, they also eat other types of meat such as lamb and beef more often. Therefore, in this type of households a varied and complete diet is consumed in terms of meat consumption, so it would be necessary to rethink joint communication strategies among the three meat sectors. Promoting rabbit meat consumption among the under 18s has several consequences, as in the future they will be in charge of household purchases or share this responsibility.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45428012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Minor, S. Boucher, L. Joudou, R. Mellet, M. Sourice, T. L. Moullec, A. Nicolier, F. Beilvert, A. Sigognault-Flochlay
In 2010, a variant of the rabbit haemorrhagic disease virus (RHDV) belonging to a new GI.2 genotype was identified in France and rapidly spread worldwide. Due to antigenic difference, new vaccines including G1.2 strains have been developed to confer adequate protection. An increase in the pathogenicity of the circulating strains was recently reported. The objective of this experimental study was to characterise the infection with a highly pathogenic GI.2/RHDV2/b isolate (2017) and assess the efficacy of Filavac VHD K C+V vaccine (Filavie) against this strain. Four and 10-wk-old specific pathogen-free rabbits were inoculated with a recommended dose of vaccine. After 7 d, controls and vaccinated rabbits were challenged and clinically monitored for 14 d. All animals were necropsied and blood, organs and urine were sampled for quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis. In adult groups, regular nasal and rectal swabbing were performed, and faeces were collected after death to monitor RNA shedding. In control groups, the challenge strain induced acute RHD between 31 and 72 h post-inoculation, with a mortality rate of 100% for kits and 89% for adult rabbits. Except for a shorter mean time to death in kits, similar clinical signs and lesions were observed between age groups. The vaccination significantly prevented all mortality, clinical signs, detection of viral RNA in serum and gross lesions in kits and adult rabbits. In adult groups, we also demonstrated that vaccine significantly protected from detectable RNA shedding via naso-conjunctival and rectal routes. Two weeks after challenge, RNA copies were not detected by PCR in the liver, spleen, lungs, kidneys, faeces and urine of vaccinated adult rabbits. The findings for kits were similar, except that very low levels of RNA were present in the liver and spleen of a few rabbits. These data show that immunisation prevented any significant viral multiplication and/or allowed a rapid clearance. We concluded that, despite the quick evolution of GI.2/RHDV2/b strains, the protection conferred by the vaccine remains adequate. In the context of coexistence of both GI.1 and GI.2 genotypes in some countries, with the circulation of multiples recombinant viruses, the vaccination should be based on the association of strains from both genotypes.
2010年，一种属于新的GI.2基因型的兔出血症病毒（RHDV）变体在法国被发现，并在全球迅速传播。由于抗原差异，包括G1.2菌株在内的新疫苗已经被开发出来，以提供足够的保护。最近报道了循环菌株的致病性增加。本实验研究的目的是描述高致病性GI.2/RHDV2/b分离株（2017）的感染特征，并评估Filavac VHD K C+V疫苗（Filavie）对该菌株的效力。用推荐剂量的疫苗接种4周龄和10周龄的无特定病原体的兔。7天后，对对照组和接种疫苗的兔子进行攻击并进行14天的临床监测。对所有动物进行尸检，并对血液、器官和尿液进行取样，进行定量逆转录聚合酶链式反应（RT-qPCR）分析。在成年组中，定期进行鼻腔和直肠拭子检查，并在死亡后收集粪便以监测RNA的脱落。在对照组中，激发菌株在接种后31至72小时诱导急性RHD，试剂盒的死亡率为100%，成年兔的死亡率为89%。除了试剂盒中的平均死亡时间较短外，不同年龄组之间观察到相似的临床症状和病变。疫苗接种显著预防了所有死亡、临床症状、血清中病毒RNA的检测以及试剂盒和成年兔的大体病变。在成年组中，我们还证明了疫苗通过鼻结膜和直肠途径显著防止可检测的RNA脱落。激发后两周，通过PCR在接种疫苗的成年兔的肝、脾、肺、肾、粪便和尿液中未检测到RNA拷贝。试剂盒的研究结果相似，只是少数兔子的肝脏和脾脏中存在非常低水平的RNA。这些数据表明，免疫预防了任何显著的病毒增殖和/或允许快速清除。我们得出的结论是，尽管GI.2/RHDV2/b毒株进化迅速，但疫苗提供的保护仍然足够。在一些国家，在GI.1和GI.2基因型共存的情况下，随着多种重组病毒的传播，疫苗接种应基于两种基因型菌株的关联。
{"title":"Rabbit haemorrhagic disease: experimental study of a recent highly pathogenic GI.2/RHDV2/b strain and evaluation of vaccine efficacy","authors":"O. Minor, S. Boucher, L. Joudou, R. Mellet, M. Sourice, T. L. Moullec, A. Nicolier, F. Beilvert, A. Sigognault-Flochlay","doi":"10.4995/wrs.2019.11082","DOIUrl":"https://doi.org/10.4995/wrs.2019.11082","url":null,"abstract":"In 2010, a variant of the rabbit haemorrhagic disease virus (RHDV) belonging to a new GI.2 genotype was identified in France and rapidly spread worldwide. Due to antigenic difference, new vaccines including G1.2 strains have been developed to confer adequate protection. An increase in the pathogenicity of the circulating strains was recently reported. The objective of this experimental study was to characterise the infection with a highly pathogenic GI.2/RHDV2/b isolate (2017) and assess the efficacy of Filavac VHD K C+V vaccine (Filavie) against this strain. Four and 10-wk-old specific pathogen-free rabbits were inoculated with a recommended dose of vaccine. After 7 d, controls and vaccinated rabbits were challenged and clinically monitored for 14 d. All animals were necropsied and blood, organs and urine were sampled for quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis. In adult groups, regular nasal and rectal swabbing were performed, and faeces were collected after death to monitor RNA shedding. In control groups, the challenge strain induced acute RHD between 31 and 72 h post-inoculation, with a mortality rate of 100% for kits and 89% for adult rabbits. Except for a shorter mean time to death in kits, similar clinical signs and lesions were observed between age groups. The vaccination significantly prevented all mortality, clinical signs, detection of viral RNA in serum and gross lesions in kits and adult rabbits. In adult groups, we also demonstrated that vaccine significantly protected from detectable RNA shedding via naso-conjunctival and rectal routes. Two weeks after challenge, RNA copies were not detected by PCR in the liver, spleen, lungs, kidneys, faeces and urine of vaccinated adult rabbits. The findings for kits were similar, except that very low levels of RNA were present in the liver and spleen of a few rabbits. These data show that immunisation prevented any significant viral multiplication and/or allowed a rapid clearance. We concluded that, despite the quick evolution of GI.2/RHDV2/b strains, the protection conferred by the vaccine remains adequate. In the context of coexistence of both GI.1 and GI.2 genotypes in some countries, with the circulation of multiples recombinant viruses, the vaccination should be based on the association of strains from both genotypes.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45261492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The 18th edition of the French Rabbit Days (Journées de la Recherche Cunicole) was organized by the association CUNICULTURE, in association with the INRA (Institut National de la Recherche Agronomique) and the ASFC (French Branch of the WRSA), in Nantes, during one and a half days 27-28 May 2019. A total of 32 peer reviewed communications, including 3 synthesis, were orally presented to about 90 attendants. The 2 most relevant subjects were 1/ studies on and possibilities of control of the viral haemorrhagic disease caused by the new RHDV2, and 2/ control of digestive disorders, particularly coccidiosis, with nonchemical feed additives or diet formulation. One part of the communications was also devoted to the efforts made for the promotion of rabbit meat in France and to the comportment of consumers in relation with rabbit meat. The full texts of the communications, in French, are available on the WRSA website at the URL : http://world-rabbit-science.com/Other-Proceedings/2019-18th%20Rabbit%20Days/000-E-Book-JRC2019.pdf
第18届法国兔日(journacimes de la Recherche Cunicole)于2019年5月27日至28日在南特举行，由CUNICULTURE协会与法国国家农学研究所(INRA)和法国WRSA分会(ASFC)联合举办。共向约90名与会者口头提交了32份经同行审查的来文，其中包括3份综合来文。2个最相关的课题是1/研究和控制由新型RHDV2引起的病毒性出血性疾病的可能性，2/用非化学饲料添加剂或日粮配方控制消化系统疾病，特别是球虫病。通信的一部分还专门讨论了为在法国推广兔肉所作的努力以及消费者在食用兔肉时的举止。来文的法文全文可在WRSA网站上查阅，网址为:http://world-rabbit-science.com/Other-Proceedings/2019-18th%20Rabbit%20Days/000-E-Book-JRC2019.pdf
{"title":"Abstracts of the 18th French Rabbit Days","authors":"C. Abstracts","doi":"10.4995/wrs.2019.11975","DOIUrl":"https://doi.org/10.4995/wrs.2019.11975","url":null,"abstract":"<p>The 18<sup>th</sup> edition of the French Rabbit Days (Journées de la Recherche Cunicole) was organized by the association CUNICULTURE, in association with the INRA (Institut National de la Recherche Agronomique) and the ASFC (French Branch of the WRSA), in Nantes, during one and a half days 27-28 May 2019. A total of 32 peer reviewed communications, including 3 synthesis, were orally presented to about 90 attendants. The 2 most relevant subjects were 1/ studies on and possibilities of control of the viral haemorrhagic disease caused by the new RHDV2, and 2/ control of digestive disorders, particularly coccidiosis, with nonchemical feed additives or diet formulation. One part of the communications was also devoted to the efforts made for the promotion of rabbit meat in France and to the comportment of consumers in relation with rabbit meat. The full texts of the communications, in French, are available on the WRSA website at the URL : <a href=\"http://world-rabbit-science.com/Other-Proceedings/2019-18th%20Rabbit%20Days/000-E-Book-JRC2019.pdf\">http://world-rabbit-science.com/Other-Proceedings/2019-18th%20Rabbit%20Days/000-E-Book-JRC2019.pdf</a></p>","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45555828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Savietto, E. Martínez-Paredes, Juan José Pascual
Environmental insults of different nature and intensity, such as fluctuation in the air temperature, which can affect access to food, its quality and diseases, are a reality in any livestock system. This is much more important when the insults occur in early life, conditioning the development and adult life of animals. In ecology, for instance, it is widely accepted that high quality offspring are more reactive against predators, occupy better territories and find more mates, resulting in longer lives and greater fitness. It is also a given that adults exposed to famine or disease as juveniles have shorter lives and produce fewer offspring. To determine whether the environment influences the development and lifetime reproductive performance of rabbit females, we designed an experiment combining two factors: nest and pubescent development. Nest development was measured by recording the average daily gain of 864 females during suckling and during their pubescent life (63 to 184 d old), and body development was conditioned by providing animals a high-energy control (C) or a fibre-rich (F) diet. However, in the course of the study, 191 of the 864 pubescent females were exposed to rabbit haemorrhagic disease (RHD). This unexpected environmental insult was considered as a third experimental factor influencing the reproductive performance of rabbit females. Contrary to expectation, fast suckling gain impairs reproductive lifespan, resulting in fewer newborn kits produced in a female lifetime. Although females on diet F lived 37 d longer than females on diet C, this difference was only perceived in their pubescent life. In addition, the exposure to RHD interacted with suckling gain (SG). Exposed females with a fast SG produced more kits as adults, but in the absence of the virus, high SG females produced fewer newborn kits. These results open new insights into the management of future breeders during nesting and pubescent life.
{"title":"Influences of environment on the development and lifetime reproductive performance in domestic rabbit females","authors":"D. Savietto, E. Martínez-Paredes, Juan José Pascual","doi":"10.4995/wrs.2019.11968","DOIUrl":"https://doi.org/10.4995/wrs.2019.11968","url":null,"abstract":"Environmental insults of different nature and intensity, such as fluctuation in the air temperature, which can affect access to food, its quality and diseases, are a reality in any livestock system. This is much more important when the insults occur in early life, conditioning the development and adult life of animals. In ecology, for instance, it is widely accepted that high quality offspring are more reactive against predators, occupy better territories and find more mates, resulting in longer lives and greater fitness. It is also a given that adults exposed to famine or disease as juveniles have shorter lives and produce fewer offspring. To determine whether the environment influences the development and lifetime reproductive performance of rabbit females, we designed an experiment combining two factors: nest and pubescent development. Nest development was measured by recording the average daily gain of 864 females during suckling and during their pubescent life (63 to 184 d old), and body development was conditioned by providing animals a high-energy control (C) or a fibre-rich (F) diet. However, in the course of the study, 191 of the 864 pubescent females were exposed to rabbit haemorrhagic disease (RHD). This unexpected environmental insult was considered as a third experimental factor influencing the reproductive performance of rabbit females. Contrary to expectation, fast suckling gain impairs reproductive lifespan, resulting in fewer newborn kits produced in a female lifetime. Although females on diet F lived 37 d longer than females on diet C, this difference was only perceived in their pubescent life. In addition, the exposure to RHD interacted with suckling gain (SG). Exposed females with a fast SG produced more kits as adults, but in the absence of the virus, high SG females produced fewer newborn kits. These results open new insights into the management of future breeders during nesting and pubescent life.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48699252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Badr, I. El-Shawaf, M. Khalil, M. Refaat, S. Ramadan
The limited rabbit resources in Egypt are threatened by the danger of extinction, whereas genetic diversity studies of native breeds could play a vital role in conservation and improvement of these breeds. In this study, 3 native rabbit breeds: Gabali (G), Baladi Red (BR) and Baladi Black (BB), in addition to New Zealand White (NZW), were genotyped using 12 microsatellite markers. All the typed microsatellites were polymorphic by average number of alleles 5.25 per locus. Observed and expected heterozygosity per locus averaged 0.62 and 0.68, respectively. The average polymorphic information content was 0.71 and the highest polymorphic information content was recorded in locus SOL33 by 0.85. All the studied loci except SAT7 and SAT2 showed deviation from Hardy-Weinberg equilibrium with significant level. The inbreeding coefficient of the individuals relative to the total population was 0.07. The within-population heterozygote deficit averaged 0.07 and ranged from 0.141 in BR to 0.015 in BB breeds. The highest pairwise differentiation among the populations was recorded between BB and NZW (0.071), while the lowest value was recorded between BR and both of G (0.038) and BB (0.039). The lowest pairwise Nei’s genetic distance was recorded between BR and BB (0.190), while the highest was recorded between NZW and BB breeds (0.409). BR and G populations were clustered together forming an admixed mosaic cluster. BR recorded the highest contribution in the aggregate genetic diversity based on the three prioritisation methods used.
{"title":"Molecular genetic diversity and conservation priorities of Egyptian rabbit breeds","authors":"O. Badr, I. El-Shawaf, M. Khalil, M. Refaat, S. Ramadan","doi":"10.4995/wrs.2019.8923","DOIUrl":"https://doi.org/10.4995/wrs.2019.8923","url":null,"abstract":"The limited rabbit resources in Egypt are threatened by the danger of extinction, whereas genetic diversity studies of native breeds could play a vital role in conservation and improvement of these breeds. In this study, 3 native rabbit breeds: Gabali (G), Baladi Red (BR) and Baladi Black (BB), in addition to New Zealand White (NZW), were genotyped using 12 microsatellite markers. All the typed microsatellites were polymorphic by average number of alleles 5.25 per locus. Observed and expected heterozygosity per locus averaged 0.62 and 0.68, respectively. The average polymorphic information content was 0.71 and the highest polymorphic information content was recorded in locus SOL33 by 0.85. All the studied loci except SAT7 and SAT2 showed deviation from Hardy-Weinberg equilibrium with significant level. The inbreeding coefficient of the individuals relative to the total population was 0.07. The within-population heterozygote deficit averaged 0.07 and ranged from 0.141 in BR to 0.015 in BB breeds. The highest pairwise differentiation among the populations was recorded between BB and NZW (0.071), while the lowest value was recorded between BR and both of G (0.038) and BB (0.039). The lowest pairwise Nei’s genetic distance was recorded between BR and BB (0.190), while the highest was recorded between NZW and BB breeds (0.409). BR and G populations were clustered together forming an admixed mosaic cluster. BR recorded the highest contribution in the aggregate genetic diversity based on the three prioritisation methods used.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45636005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vocalisations can be used as reliable indicators of pain, but little information is available in rabbits, where acoustic tools for farming environments can be used for welfare judgements. The aim of this study was to compare vocalisations produced during copula ejaculation and electro-ejaculation (EE), with or without general anaesthesia, in domestic rabbits. Vocalisations of nine New Zealand white adult males were digitally recorded. The number of males vocalising and vocal characteristics including high, low, maximum and fundamental frequencies and duration of the vocalisations were analysed. There were no differences in the number of males vocalising or any vocalisation parameter between the 1st and 2nd ejaculation while copulating, even though the fundamental frequency increased in all males in the 2nd ejaculation (P=0.008). More males vocalised while mating than while being electro-ejaculated (P=0.03), and all vocalisation parameters were greater during EE than while mating (P=0.004). The use or not of anaesthesia during EE did not modify any of the parameters evaluated. It was concluded that: 1) more males vocalised during copula ejaculation than while being electro-ejaculated; 2) bio-acoustic analysis allowed us to identify aversive utterance vocalisations, which are characterised with higher frequencies, that those from non-aversive stimulus; and 3) at least with the anaesthetic combination and the responses studied, anaesthesia had no effect on the acoustic characteristics of the vocalisation emitted during EE in rabbits.
{"title":"Acoustic characteristics of vocalisations emitted by the domestic rabbit (Oryctolagus cuniculus) during copula ejaculation and electro-ejaculation with or without anaesthesia","authors":"A. Orihuela, R. Ungerfeld","doi":"10.4995/wrs.2019.10809","DOIUrl":"https://doi.org/10.4995/wrs.2019.10809","url":null,"abstract":"Vocalisations can be used as reliable indicators of pain, but little information is available in rabbits, where acoustic tools for farming environments can be used for welfare judgements. The aim of this study was to compare vocalisations produced during copula ejaculation and electro-ejaculation (EE), with or without general anaesthesia, in domestic rabbits. Vocalisations of nine New Zealand white adult males were digitally recorded. The number of males vocalising and vocal characteristics including high, low, maximum and fundamental frequencies and duration of the vocalisations were analysed. There were no differences in the number of males vocalising or any vocalisation parameter between the 1st and 2nd ejaculation while copulating, even though the fundamental frequency increased in all males in the 2nd ejaculation (P=0.008). More males vocalised while mating than while being electro-ejaculated (P=0.03), and all vocalisation parameters were greater during EE than while mating (P=0.004). The use or not of anaesthesia during EE did not modify any of the parameters evaluated. It was concluded that: 1) more males vocalised during copula ejaculation than while being electro-ejaculated; 2) bio-acoustic analysis allowed us to identify aversive utterance vocalisations, which are characterised with higher frequencies, that those from non-aversive stimulus; and 3) at least with the anaesthetic combination and the responses studied, anaesthesia had no effect on the acoustic characteristics of the vocalisation emitted during EE in rabbits.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47470434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Sirotkin, A. Štochmaľová, R. Grossmann, S. Alwasel, A. Harrath
The bioflavonoid quercetin is a component of food with numerous biological effects, but its function in reproductive processes remains to be investigated. This study aimed to examine the direct action of quercetin on steroid hormone release in rabbit ovaries. We analysed the effect of quercetin (0, 1, 10, and 100 ng/mL) on cultured rabbit ovarian fragments. The release of progesterone (P4), testosterone (T) and estradiol (E2) were analysed by enzyme immunoassay. Quercetin promoted P4, T, and E2 release by rabbit ovarian fragments. These observations indicate that quercetin can directly stimulate rabbit ovarian steroidogenesis – an essential regulator of reproduction and fecundity. The application of dietary quercetin for control of rabbit reproduction is discussed.
{"title":"Quercetin directly promotes rabbit ovarian steroidogenesis","authors":"A. Sirotkin, A. Štochmaľová, R. Grossmann, S. Alwasel, A. Harrath","doi":"10.4995/wrs.2019.11816","DOIUrl":"https://doi.org/10.4995/wrs.2019.11816","url":null,"abstract":"The bioflavonoid quercetin is a component of food with numerous biological effects, but its function in reproductive processes remains to be investigated. This study aimed to examine the direct action of quercetin on steroid hormone release in rabbit ovaries. We analysed the effect of quercetin (0, 1, 10, and 100 ng/mL) on cultured rabbit ovarian fragments. The release of progesterone (P4), testosterone (T) and estradiol (E2) were analysed by enzyme immunoassay. Quercetin promoted P4, T, and E2 release by rabbit ovarian fragments. These observations indicate that quercetin can directly stimulate rabbit ovarian steroidogenesis – an essential regulator of reproduction and fecundity. The application of dietary quercetin for control of rabbit reproduction is discussed.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45841119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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