Haitao Sun, Y. Wang, L. Bai, Ce Liu, Yun Xu, Shuo Gao, Wenxue Jiang, Li Ping Yang, Gongshi Liu
This experiment was conducted to evaluate the effects of dietary inclusion of fermented ginger straw on the growth performance, gastrointestinal tract development and caecal fermentation of fattening rabbits. A total of 160 45-d-old Laiwu black rabbits were randomly divided into 4 groups and fed 0% (Control), 5, 10 or 15% fermented ginger straw in their diet as a replacement for peanut straw powder. The trial lasted for 7 d of adaptation and 43 d for testing. Growth performance was recorded from 52 to 95 d of age (n=5 per treatment with 30 rabbits, 3 males and 3 females per replicate), TTAD of nutrients from 91 to 95 d of age, and gastrointestinal tract development, caecum fermentation and carcass traits were determined at 95 d of age (n=5 per treatment with 10 rabbits, 1 males and 1 females in per replicate).The results showed that the average daily gain and final body weight in the experimental groups (5, 10 and 15% fermented ginger straw) were higher than in the control group (P<0.05). However, the average daily feed intake in the 15% group was higher than in the other groups, while the total tract apparent digestibility of crude protein, ether extract, neutral detergent fibre and acid detergent fibre were lower than in the control group (P<0.05), and the relative weights of the stomach, small intestine and caecum content in the 15% substitution group were higher than those in the control group (P<0.05). In addition, the thickness of the muscle layer in the 15% substitution group was higher than that in the other groups (P<0.05). Moreover, pH and total volatile fatty acids concentration in the caecal content were similar among the 4 groups (P>0.05). The current work shows that fermented ginger straw could be used as roughage material in fattening rabbit production up to a dietary dose of 10%.
{"title":"Dietary inclusion of fermented ginger straw effect on the growth performance, gastrointestinal tract development and caecal fermentation of fattening rabbits","authors":"Haitao Sun, Y. Wang, L. Bai, Ce Liu, Yun Xu, Shuo Gao, Wenxue Jiang, Li Ping Yang, Gongshi Liu","doi":"10.4995/wrs.2022.16093","DOIUrl":"https://doi.org/10.4995/wrs.2022.16093","url":null,"abstract":"This experiment was conducted to evaluate the effects of dietary inclusion of fermented ginger straw on the growth performance, gastrointestinal tract development and caecal fermentation of fattening rabbits. A total of 160 45-d-old Laiwu black rabbits were randomly divided into 4 groups and fed 0% (Control), 5, 10 or 15% fermented ginger straw in their diet as a replacement for peanut straw powder. The trial lasted for 7 d of adaptation and 43 d for testing. Growth performance was recorded from 52 to 95 d of age (n=5 per treatment with 30 rabbits, 3 males and 3 females per replicate), TTAD of nutrients from 91 to 95 d of age, and gastrointestinal tract development, caecum fermentation and carcass traits were determined at 95 d of age (n=5 per treatment with 10 rabbits, 1 males and 1 females in per replicate).The results showed that the average daily gain and final body weight in the experimental groups (5, 10 and 15% fermented ginger straw) were higher than in the control group (P<0.05). However, the average daily feed intake in the 15% group was higher than in the other groups, while the total tract apparent digestibility of crude protein, ether extract, neutral detergent fibre and acid detergent fibre were lower than in the control group (P<0.05), and the relative weights of the stomach, small intestine and caecum content in the 15% substitution group were higher than those in the control group (P<0.05). In addition, the thickness of the muscle layer in the 15% substitution group was higher than that in the other groups (P<0.05). Moreover, pH and total volatile fatty acids concentration in the caecal content were similar among the 4 groups (P>0.05). The current work shows that fermented ginger straw could be used as roughage material in fattening rabbit production up to a dietary dose of 10%.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47191657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Rouillon, S. Camugli, O. Carion, A. Echegaray, G. Delhomme, E. Schmitt
The use of fluoroquinolone antibiotics was legally restricted by the European Commission in March 2019. Since the extender for rabbit semen Galap® contained this antibiotic, it became necessary to modify it. The purpose of this study was to search for another molecule, based on its antimicrobial activity and also on the conservation, motility and fertility of semen diluted with the new extender. Several bacterial strains were isolated from 10 poor-quality ejaculates, including Enterococcus spp., Staphylococcus aureus and Proteus spp. They were then tested for antimicrobial susceptibility. Out of 15 antibiotics evaluated, gentamicin was the one targeting such bacteria. In vivo tests were then carried out to assess the effects of this antibiotic change on sperm parameters. Up to 26 pools of good quality semen (total motility >70%) were diluted in original Galap® or in this extender with gentamicin. Ejaculates were analysed on the day (D) of collection and up to 6 d of storage at +17°C. After 24 h storage, the motility in the new extender was reduced by 7.7% compared to the original; this decrease did not worsen by storing. After 6 d of storage, no difference between the two media was detected. A total of 360 females were then inseminated with pools of 4 to 5 good quality semen diluted 1:10 in both extenders, following the routine protocol of rabbit semen processing centres. There was no difference in female fertility or prolificacy between both formulations. In conclusion, these preliminary results suggest that the addition of gentamicin to this extender is useful and also has no adverse effect on fertility or prolificacy.
{"title":"Antimicrobials in a rabbit semen extender: effects on reproduction","authors":"C. Rouillon, S. Camugli, O. Carion, A. Echegaray, G. Delhomme, E. Schmitt","doi":"10.4995/wrs.2022.17132","DOIUrl":"https://doi.org/10.4995/wrs.2022.17132","url":null,"abstract":"The use of fluoroquinolone antibiotics was legally restricted by the European Commission in March 2019. Since the extender for rabbit semen Galap® contained this antibiotic, it became necessary to modify it. The purpose of this study was to search for another molecule, based on its antimicrobial activity and also on the conservation, motility and fertility of semen diluted with the new extender. Several bacterial strains were isolated from 10 poor-quality ejaculates, including Enterococcus spp., Staphylococcus aureus and Proteus spp. They were then tested for antimicrobial susceptibility. Out of 15 antibiotics evaluated, gentamicin was the one targeting such bacteria. In vivo tests were then carried out to assess the effects of this antibiotic change on sperm parameters. Up to 26 pools of good quality semen (total motility >70%) were diluted in original Galap® or in this extender with gentamicin. Ejaculates were analysed on the day (D) of collection and up to 6 d of storage at +17°C. After 24 h storage, the motility in the new extender was reduced by 7.7% compared to the original; this decrease did not worsen by storing. After 6 d of storage, no difference between the two media was detected. A total of 360 females were then inseminated with pools of 4 to 5 good quality semen diluted 1:10 in both extenders, following the routine protocol of rabbit semen processing centres. There was no difference in female fertility or prolificacy between both formulations. In conclusion, these preliminary results suggest that the addition of gentamicin to this extender is useful and also has no adverse effect on fertility or prolificacy.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42211722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Ahmed, N. Ali, M. Abdelhafez, H. Darwish, A. El-keredy
Rabbit breeds in Egypt are local and adapted foreign breeds that have been imported since the middle of the last century. Stressful environmental conditions including climatic changes, exposure to diseases and breeding selection have an influence on how gene flow has shaped the genetic diversity of the breeds. Mitochondrial DNA D-loop is a genetic marker used to trace the geographic distribution of genetic variation for the investigation of expansions, migrations and other gene flow patterns. The study aimed to determine the genetic diversity of the mitochondrial DNA D-loop (mtDNA D-loop) in Black Baladi, Red Baladi, Gabali, APRI line and New Zealand breeds to gather the scientific data required to create a proper conservation and sustainable management plan. Blood samples were taken from animals unrelated to each other. A 332-bp of mtDNA D-loop was successfully amplified and alignment sequences were deposited in the GenBank database. The results detected six haplotypes in the five breeds. Haplotype diversity within individual breeds varied from 0 (Red Baladi) to 0.551±0.114 (Gabali). The nucleotide diversity (π) value was relatively low (0.001-0.006), with greater values in APRI and New Zealand. Pairwise distances between breeds yielded varying values ranging from 0 to 0.254, and the values between the Red Baladi and other breeds were comparatively high, with pairwise distances from 0.172 to 0.254. The phylogenetic analysis involved 74 nucleotide sequences of the Egyptian rabbit and thirty-one sequences retrieved from GenBank of the reference samples of different haplogroups. The results of the phylogenetic analysis correlated to the reference mtDNA GenBank database showed that the five Egyptian rabbit breeds were grouped into haplotypes A, B and K. The results of the genetic diversity using mtDNA shed light on the importance of the local breed’s genetic diversity information and revealed unique mtDNA haplotypes, which is an important finding for breeding strategies designed to conserve genetic variants and provide sustainable management.
{"title":"Mitochondrial D-loop sequences and haplotypes diversity in Egyptian rabbit breeds","authors":"S. Ahmed, N. Ali, M. Abdelhafez, H. Darwish, A. El-keredy","doi":"10.4995/wrs.2022.17235","DOIUrl":"https://doi.org/10.4995/wrs.2022.17235","url":null,"abstract":"Rabbit breeds in Egypt are local and adapted foreign breeds that have been imported since the middle of the last century. Stressful environmental conditions including climatic changes, exposure to diseases and breeding selection have an influence on how gene flow has shaped the genetic diversity of the breeds. Mitochondrial DNA D-loop is a genetic marker used to trace the geographic distribution of genetic variation for the investigation of expansions, migrations and other gene flow patterns. The study aimed to determine the genetic diversity of the mitochondrial DNA D-loop (mtDNA D-loop) in Black Baladi, Red Baladi, Gabali, APRI line and New Zealand breeds to gather the scientific data required to create a proper conservation and sustainable management plan. Blood samples were taken from animals unrelated to each other. A 332-bp of mtDNA D-loop was successfully amplified and alignment sequences were deposited in the GenBank database. The results detected six haplotypes in the five breeds. Haplotype diversity within individual breeds varied from 0 (Red Baladi) to 0.551±0.114 (Gabali). The nucleotide diversity (π) value was relatively low (0.001-0.006), with greater values in APRI and New Zealand. Pairwise distances between breeds yielded varying values ranging from 0 to 0.254, and the values between the Red Baladi and other breeds were comparatively high, with pairwise distances from 0.172 to 0.254. The phylogenetic analysis involved 74 nucleotide sequences of the Egyptian rabbit and thirty-one sequences retrieved from GenBank of the reference samples of different haplogroups. The results of the phylogenetic analysis correlated to the reference mtDNA GenBank database showed that the five Egyptian rabbit breeds were grouped into haplotypes A, B and K. The results of the genetic diversity using mtDNA shed light on the importance of the local breed’s genetic diversity information and revealed unique mtDNA haplotypes, which is an important finding for breeding strategies designed to conserve genetic variants and provide sustainable management.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43096498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Growth and development of hair follicles (HF) is a complex and dynamic process in most mammals. As HF growth and development regulate rabbit wool yield, exploring the role of genes involved in HF growth and development may be relevant. In this study, the coding sequence of the Angora rabbit (Oryctolagus cuniculus) WIF1 gene was cloned. The length of the coding region sequence was found to be 1140 bp, which encodes 379 amino acids. Bioinformatics analysis indicated that the WIF1 protein was unstable, hydrophilic and located in the extracellular region, contained a putative signal peptide and exhibited a high homology in different mammals. Moreover, WIF1 was significantly downregulated in the high wool production in the Angora rabbit group. Overexpression and knockdown studies revealed that WIF1 regulates HF growth and development-related genes and proteins, such as LEF1 and CCND1. WIF1 activated β-catenin/TCF transcriptional activity, promoted cell apoptosis and inhibited cellular proliferation. These results indicate that WIF1 might be important for HF development. This study, therefore, provides a theoretical foundation for investigating WIF1 in HF growth and development.
{"title":"Characterisation and functional analysis of the WIF1 gene and its role in hair follicle growth and development of the Angora rabbit","authors":"Bohao Zhao, Jiali Li, Xi-Yan Zhang, Zhiyuan Bao, Yang Chen, Xinsheng Wu","doi":"10.4995/wrs.2022.17353","DOIUrl":"https://doi.org/10.4995/wrs.2022.17353","url":null,"abstract":"Growth and development of hair follicles (HF) is a complex and dynamic process in most mammals. As HF growth and development regulate rabbit wool yield, exploring the role of genes involved in HF growth and development may be relevant. In this study, the coding sequence of the Angora rabbit (Oryctolagus cuniculus) WIF1 gene was cloned. The length of the coding region sequence was found to be 1140 bp, which encodes 379 amino acids. Bioinformatics analysis indicated that the WIF1 protein was unstable, hydrophilic and located in the extracellular region, contained a putative signal peptide and exhibited a high homology in different mammals. Moreover, WIF1 was significantly downregulated in the high wool production in the Angora rabbit group. Overexpression and knockdown studies revealed that WIF1 regulates HF growth and development-related genes and proteins, such as LEF1 and CCND1. WIF1 activated β-catenin/TCF transcriptional activity, promoted cell apoptosis and inhibited cellular proliferation. These results indicate that WIF1 might be important for HF development. This study, therefore, provides a theoretical foundation for investigating WIF1 in HF growth and development.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48400828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Freezing and thawing processes result in production and accumulation of high concentrations of reactive oxygen species that are detrimental to spermatozoal motility and fertility. Therefore, supplementation of exogenous source of antioxidants to freezing diluent is crucial. The aim of the present study was to investigate for the first time whether supplementation of semen diluent with alpha linolenic acid (ALA) can improve motility, viability, membrane integrity, antioxidant status and fertility of post-thaw rabbit spermatozoa. Semen was collected and pooled from fifteen New Zealand white rabbit bucks. Semen samples were diluted with a tris-citrate-glucose (TCG) extender supplemented with ALA (0, 50, 75 and 100 μmol). Then, extended rabbit semen was cooled at 5°C and cryopreserved in liquid nitrogen. After thawing, spermatozoal quality parameters (individual motility %, viability %, osmotic resistance %, and acrosome integrity %), antioxidant activity (SOD, CAT, and GSH activities), lipid peroxidation (malondialdehyde) and fertility (conception and kindling rates) were evaluated. Results revealed that supplementation of rabbit semen extender with 50 μmol ALA significantly (P<0.05) increased spermatozoal characteristics including motility (56.54%), viability (60.01%), acrosome status (72.66%) and membrane integrity (59.13%). The activity of semen antioxidant enzymes (SOD, CAT, and GSH) showed a significant improvement with a marked decrease in lipid peroxidation. Moreover, the conception (73.30%) and kindling (70.00%) rates were significantly (P<0.05) higher in does inseminated with thawed semen treated with 50 μmol ALA in comparison with other concentrations (0, 75 and 100 μmol). In summary, supplementation of rabbit semen extender with 50 μmol ALA improved motility, viability, membrane integrity, acrosome integrity, antioxidant enzymes activity and fertility of post-thaw rabbit spermatozoa. Our findings suggested that higher concentrations of ALA are detrimental to post-thaw characteristics of New Zealand white rabbit buck spermatozoa. To achieve better results, the semen freezing extender should be supplemented with ALA at lower concentrations, especially 50 μmol.
{"title":"Influence of alpha linolenic acid on the motility, viability, antioxidant activity and fertility of frozen-thawed New Zealand white rabbit buck semen","authors":"A. Fadl, K. El-Shahat, E. Abdelnaby","doi":"10.4995/wrs.2022.17042","DOIUrl":"https://doi.org/10.4995/wrs.2022.17042","url":null,"abstract":"Freezing and thawing processes result in production and accumulation of high concentrations of reactive oxygen species that are detrimental to spermatozoal motility and fertility. Therefore, supplementation of exogenous source of antioxidants to freezing diluent is crucial. The aim of the present study was to investigate for the first time whether supplementation of semen diluent with alpha linolenic acid (ALA) can improve motility, viability, membrane integrity, antioxidant status and fertility of post-thaw rabbit spermatozoa. Semen was collected and pooled from fifteen New Zealand white rabbit bucks. Semen samples were diluted with a tris-citrate-glucose (TCG) extender supplemented with ALA (0, 50, 75 and 100 μmol). Then, extended rabbit semen was cooled at 5°C and cryopreserved in liquid nitrogen. After thawing, spermatozoal quality parameters (individual motility %, viability %, osmotic resistance %, and acrosome integrity %), antioxidant activity (SOD, CAT, and GSH activities), lipid peroxidation (malondialdehyde) and fertility (conception and kindling rates) were evaluated. Results revealed that supplementation of rabbit semen extender with 50 μmol ALA significantly (P<0.05) increased spermatozoal characteristics including motility (56.54%), viability (60.01%), acrosome status (72.66%) and membrane integrity (59.13%). The activity of semen antioxidant enzymes (SOD, CAT, and GSH) showed a significant improvement with a marked decrease in lipid peroxidation. Moreover, the conception (73.30%) and kindling (70.00%) rates were significantly (P<0.05) higher in does inseminated with thawed semen treated with 50 μmol ALA in comparison with other concentrations (0, 75 and 100 μmol). In summary, supplementation of rabbit semen extender with 50 μmol ALA improved motility, viability, membrane integrity, acrosome integrity, antioxidant enzymes activity and fertility of post-thaw rabbit spermatozoa. Our findings suggested that higher concentrations of ALA are detrimental to post-thaw characteristics of New Zealand white rabbit buck spermatozoa. To achieve better results, the semen freezing extender should be supplemented with ALA at lower concentrations, especially 50 μmol.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41815591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present study, three experiments were designed to identify the most appropriate technique for freezing rabbit semen. Experiment 1 aimed to determine the optimal levels of dimethyl sulfoxide (DMSO) contents in freezing medium and their effects on individual bucks. Semen ejaculates for each buck (n=15 bucks) were mixed and split into three portions for extension with a freezing medium containing varying concentrations of DMSO (0.75, 1.0, and 1.4 M). Diluted semen samples were packaged in 0.25 mL straws and suspended above liquid nitrogen (LN) for 10 min, then dipped in LN. A few days after freezing, post-thaw semen evaluation was assessed, and according to the results, six bucks and an extender containing 0.75 M of DMSO were used for experiments 2 and 3. In experiment 2, the pooled semen from 6 bucks was divided into two portions for packaging in two straw sizes (0.25 and 0.50 mL). Each straw size was divided into five groups and suspended at different heights above LN (2, 4, 6, 8, and 10 cm) for 10 minutes before being preserved in LN. In experiment 3, the pooled semen was divided into four portions for dilution with freezing medium containing different concentrations of bovine serum albumin (BSA; 0, 2.5, 5.0, and 7.5 mg/mL). Semen samples were packaged in a 0.50 mL straw and suspended 10 min, 4 cm above LN for freezing. Pre-freezing and post-thawing, semen samples were evaluated for semen quality. Results showed that the extender containing 0.75 M DMSO had higher significant values for post-thaw sperm motility, longevity, acrosome integrity and sperm plasma membrane permeability. Bucks’ individuality had significant effects on post-thaw motility, acrosome and sperm plasma membrane integrity. A significant interaction was recorded between DMSO concentrations and bucks’ individuality on sperm longevity. Semen package sizes had no significant effects on the evaluated parameters. Semen was frozen at 2 and 4 cm above LN had significantly better post-thaw quality. BSA at concentrations 5 and 7.5 mg/mL improved recovery rates of acrosome integrity and sperm membrane permeability. DMSO 0.75 M and freezing 4 cm above LN seem to be more adequate for rabbit semen cryopreservation. The appropriate level of DMSO differs between bucks, as the post-thaw sperm longevity is affected. BSA enhanced acrosome and sperm membrane integrity. Results obtained will need further investigation to be confirmed in the field.
{"title":"Cryopreservation of rabbit semen: impacts of permeable and non-permeable mixture of cryoprotectant, male group individuality, freezing rate, semen package size and antioxidant bovine serum albumin on rabbit semen freezability","authors":"K. Mohammed, G. Darwish, Z. Rawash, Amany M. Taha","doi":"10.4995/wrs.2022.17303","DOIUrl":"https://doi.org/10.4995/wrs.2022.17303","url":null,"abstract":"In the present study, three experiments were designed to identify the most appropriate technique for freezing rabbit semen. Experiment 1 aimed to determine the optimal levels of dimethyl sulfoxide (DMSO) contents in freezing medium and their effects on individual bucks. Semen ejaculates for each buck (n=15 bucks) were mixed and split into three portions for extension with a freezing medium containing varying concentrations of DMSO (0.75, 1.0, and 1.4 M). Diluted semen samples were packaged in 0.25 mL straws and suspended above liquid nitrogen (LN) for 10 min, then dipped in LN. A few days after freezing, post-thaw semen evaluation was assessed, and according to the results, six bucks and an extender containing 0.75 M of DMSO were used for experiments 2 and 3. In experiment 2, the pooled semen from 6 bucks was divided into two portions for packaging in two straw sizes (0.25 and 0.50 mL). Each straw size was divided into five groups and suspended at different heights above LN (2, 4, 6, 8, and 10 cm) for 10 minutes before being preserved in LN. In experiment 3, the pooled semen was divided into four portions for dilution with freezing medium containing different concentrations of bovine serum albumin (BSA; 0, 2.5, 5.0, and 7.5 mg/mL). Semen samples were packaged in a 0.50 mL straw and suspended 10 min, 4 cm above LN for freezing. Pre-freezing and post-thawing, semen samples were evaluated for semen quality. Results showed that the extender containing 0.75 M DMSO had higher significant values for post-thaw sperm motility, longevity, acrosome integrity and sperm plasma membrane permeability. Bucks’ individuality had significant effects on post-thaw motility, acrosome and sperm plasma membrane integrity. A significant interaction was recorded between DMSO concentrations and bucks’ individuality on sperm longevity. Semen package sizes had no significant effects on the evaluated parameters. Semen was frozen at 2 and 4 cm above LN had significantly better post-thaw quality. BSA at concentrations 5 and 7.5 mg/mL improved recovery rates of acrosome integrity and sperm membrane permeability. DMSO 0.75 M and freezing 4 cm above LN seem to be more adequate for rabbit semen cryopreservation. The appropriate level of DMSO differs between bucks, as the post-thaw sperm longevity is affected. BSA enhanced acrosome and sperm membrane integrity. Results obtained will need further investigation to be confirmed in the field.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48288361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was carried out to estimate general combining ability (GCA) and specific combining ability (SCA) of pre-and post-weaning traits from a partial diallel cross in three rabbit breeds: Local rabbit (L), Flemish Giant (F), and Rex (R). Body weight at 0 (BW0), 30 (BW30), 42 (BW42), and 63 (BW63) days of age, average daily gains from 0 to 30 d of age (ADG0-30), from 30 to 42 d of age (ADG30-42), from 42 to 63 d of age (ADG42-63), and from 30 to 63 d of age (ADG30-63), litter size at birth (LS0) and at weaning (LS42), and mortality at weaning (MR42) were studied in crossing LL, FF, RR, LF, LR, and FR. Local breed had the highest GCA for BW0, BW30, BW42, and average daily gain before weaning (ADG0-30, and ADG30-42) compared to Flemish Giant and Rex, while GCA of Local breed for average daily gain after weaning (ADG42-63), litter size (LS0, and LS42), and mortality (MR42) was higher than for Rex and similar to that of Flemish Giant. Crossing LF and LR showed higher SCA for BW30, BW42, BW63, ADG0-30, ADG42-63 and ADG30-63 than FR. In conclusion, based on GCA and SCA, the Indonesian Local breed has a high genetic potential in the crossing with Flemish Giant and Rex breeds.
{"title":"Partial diallel cross for assessing genetic merit of local rabbit breed","authors":"A. Setiaji, E. Kurnianto, S. Sutopo","doi":"10.4995/wrs.2022.14990","DOIUrl":"https://doi.org/10.4995/wrs.2022.14990","url":null,"abstract":"This study was carried out to estimate general combining ability (GCA) and specific combining ability (SCA) of pre-and post-weaning traits from a partial diallel cross in three rabbit breeds: Local rabbit (L), Flemish Giant (F), and Rex (R). Body weight at 0 (BW0), 30 (BW30), 42 (BW42), and 63 (BW63) days of age, average daily gains from 0 to 30 d of age (ADG0-30), from 30 to 42 d of age (ADG30-42), from 42 to 63 d of age (ADG42-63), and from 30 to 63 d of age (ADG30-63), litter size at birth (LS0) and at weaning (LS42), and mortality at weaning (MR42) were studied in crossing LL, FF, RR, LF, LR, and FR. Local breed had the highest GCA for BW0, BW30, BW42, and average daily gain before weaning (ADG0-30, and ADG30-42) compared to Flemish Giant and Rex, while GCA of Local breed for average daily gain after weaning (ADG42-63), litter size (LS0, and LS42), and mortality (MR42) was higher than for Rex and similar to that of Flemish Giant. Crossing LF and LR showed higher SCA for BW30, BW42, BW63, ADG0-30, ADG42-63 and ADG30-63 than FR. In conclusion, based on GCA and SCA, the Indonesian Local breed has a high genetic potential in the crossing with Flemish Giant and Rex breeds.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45729557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Dalle Zotte, Gianluca Pranzo, Sandro Tenti, Z. Szendrő, A. Szabó
Twenty-five Pannon White male rabbits reared and fed in similar conditions were slaughtered at 11 weeks of age. The longissimus thoracis et lumborum muscles (LTL; right and left) were removed at 24 h post-mortem and allocated to four sampling/storage treatments: the left side of LTL muscle was divided in half perpendicularly, with the posterior portion being analysed within one day (fresh), and the anterior portion vacuum packaged and stored for 1 mo at –20°C (whole-frozen); the right LTL side was ground with half of the product vacuum packaged and frozen for 1 mo at –20°C (ground-frozen), whereas the other half was freeze-dried, vacuum packaged, and stored for 1 mo at 4°C (freeze-dried refrigerated). Treatments impacted percentages of total saturated (P<0.01), monounsaturated (P<0.05), and polyunsaturated fatty acids (P<0.001), whole-frozen treatment affecting the most the fatty acids profile of the meat. Method of preparation and storage of meat samples before performing fatty acid analysis had an impact on the percentage of specific fatty acids, which could render the precision of study-to-study comparisons less reliable.
{"title":"Sample preparation and storage effects on fatty acid profile of rabbit Longissimus thoracis et lumborum muscle","authors":"A. Dalle Zotte, Gianluca Pranzo, Sandro Tenti, Z. Szendrő, A. Szabó","doi":"10.4995/wrs.2022.17160","DOIUrl":"https://doi.org/10.4995/wrs.2022.17160","url":null,"abstract":"Twenty-five Pannon White male rabbits reared and fed in similar conditions were slaughtered at 11 weeks of age. The longissimus thoracis et lumborum muscles (LTL; right and left) were removed at 24 h post-mortem and allocated to four sampling/storage treatments: the left side of LTL muscle was divided in half perpendicularly, with the posterior portion being analysed within one day (fresh), and the anterior portion vacuum packaged and stored for 1 mo at –20°C (whole-frozen); the right LTL side was ground with half of the product vacuum packaged and frozen for 1 mo at –20°C (ground-frozen), whereas the other half was freeze-dried, vacuum packaged, and stored for 1 mo at 4°C (freeze-dried refrigerated). Treatments impacted percentages of total saturated (P<0.01), monounsaturated (P<0.05), and polyunsaturated fatty acids (P<0.001), whole-frozen treatment affecting the most the fatty acids profile of the meat. Method of preparation and storage of meat samples before performing fatty acid analysis had an impact on the percentage of specific fatty acids, which could render the precision of study-to-study comparisons less reliable.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42055675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As in many African countries, the rabbit meat sector in Algeria lacks a structured market system. Very few studies have approached this issue. Thus, this paper aims to investigate the main parameters of rabbit meat marketing. To meet this objective, a descriptive survey method was provided, using a structured questionnaire, literature review and direct observation. Our study has covered the key areas of rabbit production including ten wilayas (i.e. geopolitical districts, regions, provinces, areas) situated in the northeastern part of Algeria. A survey was carried out among 32 butchers. Data were obtained through a field survey using face-to-face interviews. The result shows that the butchers are mostly men, belonging to six main wilayas; the majority of them are married, with an average age of 45 yr, and most have secondary school level or baccalaureate educational qualifications. Cross tabulations have shown a significant coefficient between location of butchers and four marketing factors i.e. rabbit selling criteria, sales volume per week, type of meat sold, and type of clientele. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) allow us to classify and to show the correlation between the different wilayas and the influential marketing factors. Rabbit meat market seems to be modest, fragmented and not organised, due to many obstacles, mainly lack of knowledge and awareness of rabbit meat, high cost production, high selling price and unavailability. Despite these setbacks, there is an opportunity to enhance rabbit marketing by increasing production, supporting breeders, reducing the selling price of rabbit and educating people about the nutritional quality of this kind of meat. To the best of our knowledge, this research is the first to create the sociogeographic profile of rabbit butchers and investigate the association between the different aspects and factors linked to rabbit meat marketing.
{"title":"Descriptive analysis of rabbit meat marketing parameters in the north-east of Algeria","authors":"Ibtissem Sanah, Abdelghani Boudjellal, S. Becila","doi":"10.4995/wrs.2022.16649","DOIUrl":"https://doi.org/10.4995/wrs.2022.16649","url":null,"abstract":"As in many African countries, the rabbit meat sector in Algeria lacks a structured market system. Very few studies have approached this issue. Thus, this paper aims to investigate the main parameters of rabbit meat marketing. To meet this objective, a descriptive survey method was provided, using a structured questionnaire, literature review and direct observation. Our study has covered the key areas of rabbit production including ten wilayas (i.e. geopolitical districts, regions, provinces, areas) situated in the northeastern part of Algeria. A survey was carried out among 32 butchers. Data were obtained through a field survey using face-to-face interviews. The result shows that the butchers are mostly men, belonging to six main wilayas; the majority of them are married, with an average age of 45 yr, and most have secondary school level or baccalaureate educational qualifications. Cross tabulations have shown a significant coefficient between location of butchers and four marketing factors i.e. rabbit selling criteria, sales volume per week, type of meat sold, and type of clientele. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) allow us to classify and to show the correlation between the different wilayas and the influential marketing factors. Rabbit meat market seems to be modest, fragmented and not organised, due to many obstacles, mainly lack of knowledge and awareness of rabbit meat, high cost production, high selling price and unavailability. Despite these setbacks, there is an opportunity to enhance rabbit marketing by increasing production, supporting breeders, reducing the selling price of rabbit and educating people about the nutritional quality of this kind of meat. To the best of our knowledge, this research is the first to create the sociogeographic profile of rabbit butchers and investigate the association between the different aspects and factors linked to rabbit meat marketing.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45454137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The study aimed to assess the growth performance, haematology, serum biochemistry, gonadal and extragonadal sperm reserves of two breeds of rabbit bucks fed dietary monosodium glutamate (MSG) at varying inclusion levels (0.00, 0.25, 0.50 and 0.75 g/kg diet). A total of 320 sexually mature New Zealand White Bucks and Dutch Belted Bucks aged 8 to 10 mo with average weight ranging from 1.34 to 1.96 kg were used for the study, which lasted 8 wk. The bucks were weighed and distributed to the four treatment diets. Each treatment was replicated 10 times with four bucks per replicate in a 2×4 factorial experiment. At the end of the feeding trial, 2 bucks per replicate were euthanised. Blood samples were collected from the jugular veins for haematological and serum analyses and their reproductive tracts were dissected. The testes and epididymides were carefully sampled, weighed and processed. The results showed that the bucks fed the diet containing 0.25 g MSG/kg had the best (P<0.05) feed conversion ratio and daily weight gain, daily sperm production and sperm production efficiency. The inclusions of up to 0.75 g MSG/kg diet did not compromise the bucks’ health status, performance and reproductive potential, irrespective of their breeds. However, optimum performance and sperm production were recorded at 0.25 g MSG/kg diet. This study suggests that dietary MSG at 0.25 g/kg in diet can significantly improve rabbit feed palatability, thereby bringing about optimum growth performance, sperm production, and efficiency without causing any physiological imbalance ino the bucks.
{"title":"Performance, haemato-biochemical and reproductive potential indices of New Zealand White and Dutch Belted rabbit bucks fed diets containing monosodium glutamate","authors":"O. Adu, O. J. Olarotimi, C. A. Chineke","doi":"10.4995/wrs.2022.15683","DOIUrl":"https://doi.org/10.4995/wrs.2022.15683","url":null,"abstract":"The study aimed to assess the growth performance, haematology, serum biochemistry, gonadal and extragonadal sperm reserves of two breeds of rabbit bucks fed dietary monosodium glutamate (MSG) at varying inclusion levels (0.00, 0.25, 0.50 and 0.75 g/kg diet). A total of 320 sexually mature New Zealand White Bucks and Dutch Belted Bucks aged 8 to 10 mo with average weight ranging from 1.34 to 1.96 kg were used for the study, which lasted 8 wk. The bucks were weighed and distributed to the four treatment diets. Each treatment was replicated 10 times with four bucks per replicate in a 2×4 factorial experiment. At the end of the feeding trial, 2 bucks per replicate were euthanised. Blood samples were collected from the jugular veins for haematological and serum analyses and their reproductive tracts were dissected. The testes and epididymides were carefully sampled, weighed and processed. The results showed that the bucks fed the diet containing 0.25 g MSG/kg had the best (P<0.05) feed conversion ratio and daily weight gain, daily sperm production and sperm production efficiency. The inclusions of up to 0.75 g MSG/kg diet did not compromise the bucks’ health status, performance and reproductive potential, irrespective of their breeds. However, optimum performance and sperm production were recorded at 0.25 g MSG/kg diet. This study suggests that dietary MSG at 0.25 g/kg in diet can significantly improve rabbit feed palatability, thereby bringing about optimum growth performance, sperm production, and efficiency without causing any physiological imbalance ino the bucks.","PeriodicalId":23902,"journal":{"name":"World Rabbit Science","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46524724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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