R. A. Gaifutdinov, Yu. O. Kuyanova, D. A. Khelimsky, O. Krestyaninov, A. A. Tulupov, D. Parshin
Coronary artery disease is a widespread cause of death and disability in the population. Angioplasty of the coronary arteries is one of the most common methods of eliminating the cause of ischemia – stenosis of the coronary arteries. As a result of stent installation, a change in the angle of vascular bifurcation occurs usually, as well as a redistribution of volumetric blood flow in the coronary artery system. Considering the high variability of the branching angioarchitecture of these arteries, as well as the structure of their environment, the problem of predicting the specific redistribution of blood flow in these arteries remains unsolved; the main ways of its implementation are computational and experimental hemodynamics. Material and methods. This paper uses an experimental approach to explore the effect of stent placement in a model of coronary artery stenosis, and also provides an analysis of the current level of awareness of the scientific community on this issue. Results and discussion. The experiment showed that the throughput of the model increases by 14 % compared to the model with stenosis, and the redistribution of flows in the model depends not on diameters but on the anatomy of a particular vascular network. The data of the performed mathematical modeling are generally consistent with the results of the experiment before stent installation, when the coronary tree consists of several load-bearing branches, but have quantitative differences for the distal branches of the coronary artery model in the presence of an installed stent. Conclusions. The results of the work can be used to accumulate an experimental data array on the restructuring of blood flow during angioplasty, and can also be used to verify the numerical hemodynamics of the coronary arteries during the virtual installation of a stent in them to resolve stenosis.
{"title":"Towards effects from stent implantation into coronary bifurcation stenosis: experiment and simulation","authors":"R. A. Gaifutdinov, Yu. O. Kuyanova, D. A. Khelimsky, O. Krestyaninov, A. A. Tulupov, D. Parshin","doi":"10.18699/ssmj20240103","DOIUrl":"https://doi.org/10.18699/ssmj20240103","url":null,"abstract":"Coronary artery disease is a widespread cause of death and disability in the population. Angioplasty of the coronary arteries is one of the most common methods of eliminating the cause of ischemia – stenosis of the coronary arteries. As a result of stent installation, a change in the angle of vascular bifurcation occurs usually, as well as a redistribution of volumetric blood flow in the coronary artery system. Considering the high variability of the branching angioarchitecture of these arteries, as well as the structure of their environment, the problem of predicting the specific redistribution of blood flow in these arteries remains unsolved; the main ways of its implementation are computational and experimental hemodynamics. Material and methods. This paper uses an experimental approach to explore the effect of stent placement in a model of coronary artery stenosis, and also provides an analysis of the current level of awareness of the scientific community on this issue. Results and discussion. The experiment showed that the throughput of the model increases by 14 % compared to the model with stenosis, and the redistribution of flows in the model depends not on diameters but on the anatomy of a particular vascular network. The data of the performed mathematical modeling are generally consistent with the results of the experiment before stent installation, when the coronary tree consists of several load-bearing branches, but have quantitative differences for the distal branches of the coronary artery model in the presence of an installed stent. Conclusions. The results of the work can be used to accumulate an experimental data array on the restructuring of blood flow during angioplasty, and can also be used to verify the numerical hemodynamics of the coronary arteries during the virtual installation of a stent in them to resolve stenosis. ","PeriodicalId":24058,"journal":{"name":"Сибирский научный медицинский журнал","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140427097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Valova, A. E. Akulov, O. Bogomyakova, A. A. Tulupov, A. A. Cherevko
Aim of the study was to compare the results of mathematical modelling of the dependence between brain ventricle size and capillary pressure for humans and animals based on the equations of multicomponent poroelastic filtration for brain parenchyma. Material and methods. The study included two groups of animals - 4 male mice of each inbred line C57Bl/6 and BALB/C at the age of 12 weeks – and 4 healthy volunteers. The brain and cerebrospinal fluid system images of mice were obtained using an 11.7 T horizontal MR scanner, group of humans were examined using the Ingenia 3.0 T MRI scanner. An axial section at the level of –0.5 mm from bregma in the mouse groups and a frontal slice at the level of the middle of the bodies of the lateral and third ventricles, posterior to the foramen of Monroe in the human group were chosen as the geometry for mathematical modelling. Mathematical modelling is based on the stationary mathematical model of multicomponent poroelastic filtration. Multiple linear regression of mean ventricular wall displacement on fluid media interaction parameters was constructed to compare results obtained. Regression coefficients were compared using nonparametric analysis of variance based on the Kraskell–Wallis criterion and post-hoc Dunn’s criterion with Hill’s correction Results. A qualitative coincidence in the behavior of capillary pressure and mean ventricular wall displacement was demonstrated for the human and mouse groups. No significant differences were found between the two animal lines. For the animals characterized by small ventricular size (BALB/c), greater similarity to humans is observed than for the genetic line with hypertrophied ventricles (C57Bl/6). A significant difference between humans and mice is observed only for capillary-venous interaction. Conclusions. The low variance within groups and insignificant discrepancy between groups indicate the possibility of further accumulation of empirical data to establish correction coefficients of the animal model, which will bring it more in line with the model for humans. Thus, the analyzed models are sufficiently comparable with each other.
{"title":"Comparison of the mathematical modelling results of the relationship between cerebral ventricular size and capillary pressure based on experimental and clinical data","authors":"G. Valova, A. E. Akulov, O. Bogomyakova, A. A. Tulupov, A. A. Cherevko","doi":"10.18699/ssmj20240102","DOIUrl":"https://doi.org/10.18699/ssmj20240102","url":null,"abstract":"Aim of the study was to compare the results of mathematical modelling of the dependence between brain ventricle size and capillary pressure for humans and animals based on the equations of multicomponent poroelastic filtration for brain parenchyma. Material and methods. The study included two groups of animals - 4 male mice of each inbred line C57Bl/6 and BALB/C at the age of 12 weeks – and 4 healthy volunteers. The brain and cerebrospinal fluid system images of mice were obtained using an 11.7 T horizontal MR scanner, group of humans were examined using the Ingenia 3.0 T MRI scanner. An axial section at the level of –0.5 mm from bregma in the mouse groups and a frontal slice at the level of the middle of the bodies of the lateral and third ventricles, posterior to the foramen of Monroe in the human group were chosen as the geometry for mathematical modelling. Mathematical modelling is based on the stationary mathematical model of multicomponent poroelastic filtration. Multiple linear regression of mean ventricular wall displacement on fluid media interaction parameters was constructed to compare results obtained. Regression coefficients were compared using nonparametric analysis of variance based on the Kraskell–Wallis criterion and post-hoc Dunn’s criterion with Hill’s correction Results. A qualitative coincidence in the behavior of capillary pressure and mean ventricular wall displacement was demonstrated for the human and mouse groups. No significant differences were found between the two animal lines. For the animals characterized by small ventricular size (BALB/c), greater similarity to humans is observed than for the genetic line with hypertrophied ventricles (C57Bl/6). A significant difference between humans and mice is observed only for capillary-venous interaction. Conclusions. The low variance within groups and insignificant discrepancy between groups indicate the possibility of further accumulation of empirical data to establish correction coefficients of the animal model, which will bring it more in line with the model for humans. Thus, the analyzed models are sufficiently comparable with each other.","PeriodicalId":24058,"journal":{"name":"Сибирский научный медицинский журнал","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140424062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. I. Chumasov, N. Maistrenko, P. N. Romashchenko, V. Samedov, E. Petrova, D. Korzhevskii
The origin, development and differentiation of enteric nervous system neuroglia and its involvement in the pathogenesis of gastrointestinal diseases and neurodegenerative diseases have been little studied.Aim of this work is a comparative morphological study of glial cells in the ganglionic plexuses of the enteric nervous system and analysis of neuroglial relationships in chronic slow-transit constipation using immunohistochemical methods.Material and methods. Resection material obtained at the Department of Faculty Surgery, S.P. Fedorov Faculty of Surgery of S.M. Kirov Military Medical Academy during planned surgical operations was used. The objects of the study were fragments of the sigmoid and colon obtained as a result of surgery for chronic slow-transit constipation (five cases, women aged 37–40 years). The study was carried out using immunohistochemical glial markers (GFAP, S100β protein, etc.).Results. Two types of glia were found in the myenteric ganglionic plexus of the large intestine: astrocyte-like and neurolemmocytic. The astrocyte-like type is similar to the neuroglia of the central nervous system, the neurolemmocytic type is similar to the glia of the autonomic nervous system. It has been established that astrocyte-like glia is found only in the Aauerbach ganglionic plexus, while neurolemmocytes are found in all innervated tissues of the intestinal wall. Reactive, dystrophic and degenerative changes in neurocytes, glial elements, agangliogenosis in the Auerbach plexus were found in all cases of chronic slow-transit constipation. Destructive changes in the neuromuscular terminal plexuses, interstitial edema and inflammatory monocytic reaction and leukocyte infiltration in the intestinal mucosa and intestinal submucosa, found in several cases.Conclusions. The results obtained allow classifying chronic slow-transit constipation as a neurodegenerative disease.
{"title":"Pathological changes of glial cells in the enteric nervous system of the colon with chronic slow-transit constipation","authors":"E. I. Chumasov, N. Maistrenko, P. N. Romashchenko, V. Samedov, E. Petrova, D. Korzhevskii","doi":"10.18699/ssmj20230624","DOIUrl":"https://doi.org/10.18699/ssmj20230624","url":null,"abstract":"The origin, development and differentiation of enteric nervous system neuroglia and its involvement in the pathogenesis of gastrointestinal diseases and neurodegenerative diseases have been little studied.Aim of this work is a comparative morphological study of glial cells in the ganglionic plexuses of the enteric nervous system and analysis of neuroglial relationships in chronic slow-transit constipation using immunohistochemical methods.Material and methods. Resection material obtained at the Department of Faculty Surgery, S.P. Fedorov Faculty of Surgery of S.M. Kirov Military Medical Academy during planned surgical operations was used. The objects of the study were fragments of the sigmoid and colon obtained as a result of surgery for chronic slow-transit constipation (five cases, women aged 37–40 years). The study was carried out using immunohistochemical glial markers (GFAP, S100β protein, etc.).Results. Two types of glia were found in the myenteric ganglionic plexus of the large intestine: astrocyte-like and neurolemmocytic. The astrocyte-like type is similar to the neuroglia of the central nervous system, the neurolemmocytic type is similar to the glia of the autonomic nervous system. It has been established that astrocyte-like glia is found only in the Aauerbach ganglionic plexus, while neurolemmocytes are found in all innervated tissues of the intestinal wall. Reactive, dystrophic and degenerative changes in neurocytes, glial elements, agangliogenosis in the Auerbach plexus were found in all cases of chronic slow-transit constipation. Destructive changes in the neuromuscular terminal plexuses, interstitial edema and inflammatory monocytic reaction and leukocyte infiltration in the intestinal mucosa and intestinal submucosa, found in several cases.Conclusions. The results obtained allow classifying chronic slow-transit constipation as a neurodegenerative disease.","PeriodicalId":24058,"journal":{"name":"Сибирский научный медицинский журнал","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139534303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Sinitskaya, M. Khutornaya, O. Khryachkova, A. O. Poddubnyak, M. Sinitsky, M. A. Asanov, A. Ponasenko
Aim of the study was to investigate the associations between TLR gene polymorphism and the risk of rheumatic heart disease (RHD).Material and methods. 251 RHD patients (190 females and 61 males) and 300 healthy donors (190 females and 110 males), whose age was 57 [29; 77] and 53 [21; 80] years (median [lower quartile; upper quartile]), respectively, were recruited in the presented study. Eight polymorphic variants in the TLR gene were genotyped by realtime PCR.Results. We found no statistically significant differences in the frequency of eight polymorphic variants in the TLR1, TLR2, TLR4 and TLR6 genes in the general group of patients. Stratification by gender and age showed that the frequency of the C/C genotype of the TLR1 gene (rs5743551) was increased in females with RHD (11.6 %) compared to the control group (5.3 %); a risk effect was determined for recessive inheritance patterns (odds ratio 2.43, 95 % confidence interval 1.07–5.52, p = 0.029). Moreover, the combination of polymorphic variants TLR2 (rs5743708) – TLR4 (rs4689791) – TLR1 (rs5743551) – TLR2 (rs3804099) showed the greatest significance in RHD risk.Conclusions. Future research on the different populations will allow to discover the general patterns of RHD pathogenesis, which will finally lead to the establishment of therapeutic targets for treatment of streptococcal infection and RHD prevention.
{"title":"Associations of TLR gene polymorphism with the risk of rheumatic heart disease","authors":"A. Sinitskaya, M. Khutornaya, O. Khryachkova, A. O. Poddubnyak, M. Sinitsky, M. A. Asanov, A. Ponasenko","doi":"10.18699/ssmj20230622","DOIUrl":"https://doi.org/10.18699/ssmj20230622","url":null,"abstract":"Aim of the study was to investigate the associations between TLR gene polymorphism and the risk of rheumatic heart disease (RHD).Material and methods. 251 RHD patients (190 females and 61 males) and 300 healthy donors (190 females and 110 males), whose age was 57 [29; 77] and 53 [21; 80] years (median [lower quartile; upper quartile]), respectively, were recruited in the presented study. Eight polymorphic variants in the TLR gene were genotyped by realtime PCR.Results. We found no statistically significant differences in the frequency of eight polymorphic variants in the TLR1, TLR2, TLR4 and TLR6 genes in the general group of patients. Stratification by gender and age showed that the frequency of the C/C genotype of the TLR1 gene (rs5743551) was increased in females with RHD (11.6 %) compared to the control group (5.3 %); a risk effect was determined for recessive inheritance patterns (odds ratio 2.43, 95 % confidence interval 1.07–5.52, p = 0.029). Moreover, the combination of polymorphic variants TLR2 (rs5743708) – TLR4 (rs4689791) – TLR1 (rs5743551) – TLR2 (rs3804099) showed the greatest significance in RHD risk.Conclusions. Future research on the different populations will allow to discover the general patterns of RHD pathogenesis, which will finally lead to the establishment of therapeutic targets for treatment of streptococcal infection and RHD prevention.","PeriodicalId":24058,"journal":{"name":"Сибирский научный медицинский журнал","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. L. Strokova, N. Pakhomova, A. I. Shevchenko, A. A. Korytkin, V. V. Kozhevnikov, А. M. Zaidman
Funnel-shaped deformity of the chest is a serious disease with an unknown etiology and pathogenesis. Pathological changes occurring in the cartilaginous tissue of the ribs are one of the leading mechanisms for the development of chest curvature. Analysis of the phenotypic features of cells isolated from the site of localization of the pathological process will create a more complete picture of the deformation development.Aim of the study was to identify the phenotype of rib cartilage cells in funnel-shaped deformation of the thorax.Material and methods. Rib cartilage cells of children with funnel-shaped chest deformity were cultured up to the 2–3rd passage, then were identified by histochemical and immunohistochemical methods.Results and discussion. The study revealed cells of two different phenotypes. The first type is oval and polygonal chondroblasts expressing a marker of chondrogenic differentiation. The second type is the cells of the neural phenotype with the expression of early neural markers Musashi1 and PAX6 and the neurospecific protein III β-tubulin, as well as the substance Nissl.Conclusions. The analysis of the phenotypic features of rib cartilage cells with funnel-shaped curvature of the chest allowed us to expand the idea of a possible etiological factor in the deformation development.
胸部漏斗状畸形是一种病因和发病机制不明的严重疾病。肋骨软骨组织发生的病理变化是导致胸部弯曲的主要机制之一。分析从病理过程定位部位分离出的细胞的表型特征将能更全面地了解变形的发展过程。研究目的是确定胸部漏斗状变形中肋软骨细胞的表型。将漏斗状胸部畸形患儿的肋软骨细胞培养至第 2-3 期，然后通过组织化学和免疫组化方法进行鉴定。研究发现了两种不同表型的细胞。第一种是表达软骨分化标记的椭圆形和多角形软骨母细胞。第二种是神经表型细胞，表达早期神经标志物 Musashi1 和 PAX6、神经特异性蛋白 III β-tubulin，以及 Nissl 物质。通过对胸部漏斗状弯曲的肋软骨细胞表型特征的分析，我们拓展了畸形发生的可能病因的思路。
{"title":"Phenotypic features of rib cartilage cells in funnel-shaped chest deformity","authors":"E. L. Strokova, N. Pakhomova, A. I. Shevchenko, A. A. Korytkin, V. V. Kozhevnikov, А. M. Zaidman","doi":"10.18699/ssmj20230625","DOIUrl":"https://doi.org/10.18699/ssmj20230625","url":null,"abstract":"Funnel-shaped deformity of the chest is a serious disease with an unknown etiology and pathogenesis. Pathological changes occurring in the cartilaginous tissue of the ribs are one of the leading mechanisms for the development of chest curvature. Analysis of the phenotypic features of cells isolated from the site of localization of the pathological process will create a more complete picture of the deformation development.Aim of the study was to identify the phenotype of rib cartilage cells in funnel-shaped deformation of the thorax.Material and methods. Rib cartilage cells of children with funnel-shaped chest deformity were cultured up to the 2–3rd passage, then were identified by histochemical and immunohistochemical methods.Results and discussion. The study revealed cells of two different phenotypes. The first type is oval and polygonal chondroblasts expressing a marker of chondrogenic differentiation. The second type is the cells of the neural phenotype with the expression of early neural markers Musashi1 and PAX6 and the neurospecific protein III β-tubulin, as well as the substance Nissl.Conclusions. The analysis of the phenotypic features of rib cartilage cells with funnel-shaped curvature of the chest allowed us to expand the idea of a possible etiological factor in the deformation development.","PeriodicalId":24058,"journal":{"name":"Сибирский научный медицинский журнал","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. H. Al-Humairi, D. L. Speranskiy, M. Y. Minakova, N. Cherdyntseva, V. V. Udut
The aim of the work is to study the effect of new azoloazine derivatives on the level of DNA damage to Vero cells (DNA comet test) in vitro by alkaline gel electrophoresis.Material and methods. The objects of the study are 8-(piperidinocarbonyl)3-cyclohexylimidazo[5,1-d][1,2,3,5]tetrazine-4(3H)-one (1), diethyl ether 4-aminoimidazo[5,1-c][1,2,4]triazine3,8-dicarboxylic acid (2), 4-amino-8-ethoxycarbonylimidazo[5,1-c][1,2,4]triazine-3-N-(p-toluyl)carboxamide (3). Epirubicin was chosen as a comparison drug. The compounds were used in doses of 1/2, 1/10 and 1/50 IC50. The Vero cell line cultured according to the standard protocol was selected as the cell model. To assess genotoxicity, an alkaline version of the DNA comet method was used, which has a high sensitivity and allows detecting DNA damage.Results. Analysis of the data obtained indicates that the tested compounds 1-3 enhance DNA damage in non-tumor cells. Compound 1 has the most pronounced genotoxic effect. Thus, the use of this substance in a dose of ½ IC50 led to a significant increase in the length of the comet’s tail by 1.5 times. It was noted that DNA damage under the action of compound 1 in the studied doses and of epirubicin was on the same level.Conclusions. The results obtained prove that compounds 1-3 may have a potentially carcinogenic effect. However, this assumption requires further in-depth experimental studies.
这项研究的目的是通过碱性凝胶电泳法研究新型氮丙嗪衍生物对体外 Vero 细胞 DNA 损伤程度（DNA 彗星试验）的影响。研究对象为 8-(哌啶甲酰基)3-环己基咪唑并[5,1-d][1,2,3,5]四嗪-4(3H)-酮（1）、二乙醚 4-氨基咪唑并[5、1-c][1,2,4]三嗪-3,8-二羧酸 (2)、4-氨基-8-乙氧羰基咪唑并[5,1-c][1,2,4]三嗪-3-N-(对甲苯基)甲酰胺 (3)。表柔比星被选为对比药物。这些化合物的 IC50 剂量分别为 1/2、1/10 和 1/50。选择按照标准方案培养的 Vero 细胞系作为细胞模型。为了评估遗传毒性，使用了碱性版 DNA 彗星法，该方法灵敏度高，可检测 DNA 损伤。对所得数据的分析表明，测试的 1-3 号化合物会增强非肿瘤细胞的 DNA 损伤。化合物 1 的基因毒性作用最为明显。因此，使用 IC50 ½ 剂量的这种物质会导致彗尾长度显著增加 1.5 倍。我们注意到，在所研究剂量的化合物 1 和表柔比星的作用下，DNA 损伤程度相同。研究结果证明，化合物 1-3 可能具有潜在的致癌作用。不过，这一假设还需要进一步的深入实验研究。
{"title":"Effect of new antitumor compounds based on azoloazine derivatives on the level of DNA damage in normal Vero cells in the DNA comet test","authors":"A. H. Al-Humairi, D. L. Speranskiy, M. Y. Minakova, N. Cherdyntseva, V. V. Udut","doi":"10.18699/ssmj20230614","DOIUrl":"https://doi.org/10.18699/ssmj20230614","url":null,"abstract":"The aim of the work is to study the effect of new azoloazine derivatives on the level of DNA damage to Vero cells (DNA comet test) in vitro by alkaline gel electrophoresis.Material and methods. The objects of the study are 8-(piperidinocarbonyl)3-cyclohexylimidazo[5,1-d][1,2,3,5]tetrazine-4(3H)-one (1), diethyl ether 4-aminoimidazo[5,1-c][1,2,4]triazine3,8-dicarboxylic acid (2), 4-amino-8-ethoxycarbonylimidazo[5,1-c][1,2,4]triazine-3-N-(p-toluyl)carboxamide (3). Epirubicin was chosen as a comparison drug. The compounds were used in doses of 1/2, 1/10 and 1/50 IC50. The Vero cell line cultured according to the standard protocol was selected as the cell model. To assess genotoxicity, an alkaline version of the DNA comet method was used, which has a high sensitivity and allows detecting DNA damage.Results. Analysis of the data obtained indicates that the tested compounds 1-3 enhance DNA damage in non-tumor cells. Compound 1 has the most pronounced genotoxic effect. Thus, the use of this substance in a dose of ½ IC50 led to a significant increase in the length of the comet’s tail by 1.5 times. It was noted that DNA damage under the action of compound 1 in the studied doses and of epirubicin was on the same level.Conclusions. The results obtained prove that compounds 1-3 may have a potentially carcinogenic effect. However, this assumption requires further in-depth experimental studies.","PeriodicalId":24058,"journal":{"name":"Сибирский научный медицинский журнал","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139626148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ya. A. Krasner, V. V. Romanov, O. N. Fazullina, M. F. Osipenko, V. Klimontov
Aim of the study was to estimate the blood serum level of microtubule-associated protein 1 light chain 3 alpha (MAP1LC3alpha), beclin-1 (BECN-1), Bcl-2 (BCL-2), caspase-3 (CASP3) and autophagy related 5 (ATG-5) protein as potential markers of liver fibrosis in type 2 diabetes (T2D) patients with non-alcoholic fatty liver disease (NAFLD).Material and methods. A single center cross-sectional observational study was performed. Seventy four patients with T2D and NAFLD were included, 23 men and 51 women, aged 18 to 74 years. Transient elastography was used for the assessment of liver fibrosis. The severity of fibrosis was estimated using the Metavir sсore. In addition, indirect liver fibrosis indices APRI and FIB-4 were calculated. Serum concentration of MAP1LC3alpha, BECN-1, BCL-2, CASP and ATG-5 was measured by ELISA. We applied ROC analysis to assess the value of studied indicators in diagnosis of liver fibrosis.Results. Liver fibrosis stage F1 was verified in 16 subjects, stage F2 in 12, stage F3 in 7, and stage F4 in 19 individuals. Patients with advanced liver fibrosis (stage 3-4) when compared to those without fibrosis (stage 0–2) had higher content of MAP1LC3alpha (p = 0.01) and BECN-1 (p = 0.01). There were no significant differences in the level of BCL-2, CASP3 and ATG-5 between patients with different severity of fibrosis. In the ROC analysis, MAP1LC3alpha and BECN-1 showed significance for the diagnosis of severe liver fibrosis, which, however, did not exceed that of the APRI index.Conclusions. In patients with T2D and NAFLD, serum levels of MAP1LC3alpha and BECN-1 are associated with severe liver fibrosis.
{"title":"Serum level of proteins involved in apoptosis and autophagy in patients with type 2 diabetes and non-alcoholic fatty liver disease","authors":"Ya. A. Krasner, V. V. Romanov, O. N. Fazullina, M. F. Osipenko, V. Klimontov","doi":"10.18699/ssmj20230618","DOIUrl":"https://doi.org/10.18699/ssmj20230618","url":null,"abstract":"Aim of the study was to estimate the blood serum level of microtubule-associated protein 1 light chain 3 alpha (MAP1LC3alpha), beclin-1 (BECN-1), Bcl-2 (BCL-2), caspase-3 (CASP3) and autophagy related 5 (ATG-5) protein as potential markers of liver fibrosis in type 2 diabetes (T2D) patients with non-alcoholic fatty liver disease (NAFLD).Material and methods. A single center cross-sectional observational study was performed. Seventy four patients with T2D and NAFLD were included, 23 men and 51 women, aged 18 to 74 years. Transient elastography was used for the assessment of liver fibrosis. The severity of fibrosis was estimated using the Metavir sсore. In addition, indirect liver fibrosis indices APRI and FIB-4 were calculated. Serum concentration of MAP1LC3alpha, BECN-1, BCL-2, CASP and ATG-5 was measured by ELISA. We applied ROC analysis to assess the value of studied indicators in diagnosis of liver fibrosis.Results. Liver fibrosis stage F1 was verified in 16 subjects, stage F2 in 12, stage F3 in 7, and stage F4 in 19 individuals. Patients with advanced liver fibrosis (stage 3-4) when compared to those without fibrosis (stage 0–2) had higher content of MAP1LC3alpha (p = 0.01) and BECN-1 (p = 0.01). There were no significant differences in the level of BCL-2, CASP3 and ATG-5 between patients with different severity of fibrosis. In the ROC analysis, MAP1LC3alpha and BECN-1 showed significance for the diagnosis of severe liver fibrosis, which, however, did not exceed that of the APRI index.Conclusions. In patients with T2D and NAFLD, serum levels of MAP1LC3alpha and BECN-1 are associated with severe liver fibrosis.","PeriodicalId":24058,"journal":{"name":"Сибирский научный медицинский журнал","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139533634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Currently the “rejuvenation” and an increase in the number of components of the metabolic syndrome (MS) determine its clinical significance in the earlier development of diabetes mellitus, cardiovascular diseases and related complications. Aim of the study was to investigate the features of the MS clinic picture in a population of young men and womenliving in the Khanty-Mansiysk autonomous region – Yugra.Material and methods. The study included 863 young people between 18 and 44 years of age, including 344 men and women with MS and 519 people in the comparison group. Studied subgroups are represented by non-indigenous men and women living in urban and rural areas, and indigenous rural residents. The analysis of MS clinical and laboratory parameters was carried out and its clinical variants in ethnic groups were identified.Results and its discussion. According to the results of the survey of young people with metabolic syndrome, it was revealed that the combination of abdominal obesity and two components of MS were most common in young people with MS (in 50.0 % men and in 55.5 % women). The study defined regression models for each MS group and threshold values for waist circumference were set, which changes in lipid and carbohydrate metabolism were predicted.Conclusions. In groups with MS, hypertriglyceridemia was determined as its most common component. The study identified the most frequent association of abdominal obesity with hyper-LDL cholesterol and hypertriglyceridemia in patients with metabolic syndrome, in ethnic groups, this combination is more common in indigenous rural men (53.3 %) and in non-indigenous urban women (54.3 %).
目前，代谢综合征（MS）的 "年轻化 "和成分数量的增加决定了其在糖尿病、心血管疾病和相关并发症的早期发展中的临床意义。本研究的目的是调查生活在汉特-曼西民族自治区尤格拉地区的青年男女中代谢综合征的临床特征。研究对象包括 863 名 18 至 44 岁的年轻人，其中包括 344 名多发性硬化症患者和 519 名对比组患者。被研究的亚组包括居住在城市和农村地区的非土著男性和女性，以及农村土著居民。对多发性硬化症的临床和实验室参数进行了分析，并确定了其在各民族群体中的临床变异。根据对患有代谢综合征的年轻人的调查结果，发现在患有 MS 的年轻人中，腹部肥胖和 MS 的两个组成部分结合在一起是最常见的（男性占 50.0%，女性占 55.5%）。研究为每个多发性硬化症群体定义了回归模型，并设定了腰围阈值，据此预测脂质和碳水化合物代谢的变化。在多发性硬化症群体中，高甘油三酯血症是最常见的组成部分。研究发现，在代谢综合征患者中，腹部肥胖与高低密度脂蛋白胆固醇和高甘油三酯血症的关系最为密切，在各族裔群体中，这种组合在农村原住民男性（53.3%）和城市非原住民女性（54.3%）中更为常见。
{"title":"Clinical variants of the metabolic syndrome in young people living in the Khanty-Mansiysk autonomous region – Yugra","authors":"E. V. Korneeva, M. I. Voevoda","doi":"10.18699/ssmj20230617","DOIUrl":"https://doi.org/10.18699/ssmj20230617","url":null,"abstract":"Currently the “rejuvenation” and an increase in the number of components of the metabolic syndrome (MS) determine its clinical significance in the earlier development of diabetes mellitus, cardiovascular diseases and related complications. Aim of the study was to investigate the features of the MS clinic picture in a population of young men and womenliving in the Khanty-Mansiysk autonomous region – Yugra.Material and methods. The study included 863 young people between 18 and 44 years of age, including 344 men and women with MS and 519 people in the comparison group. Studied subgroups are represented by non-indigenous men and women living in urban and rural areas, and indigenous rural residents. The analysis of MS clinical and laboratory parameters was carried out and its clinical variants in ethnic groups were identified.Results and its discussion. According to the results of the survey of young people with metabolic syndrome, it was revealed that the combination of abdominal obesity and two components of MS were most common in young people with MS (in 50.0 % men and in 55.5 % women). The study defined regression models for each MS group and threshold values for waist circumference were set, which changes in lipid and carbohydrate metabolism were predicted.Conclusions. In groups with MS, hypertriglyceridemia was determined as its most common component. The study identified the most frequent association of abdominal obesity with hyper-LDL cholesterol and hypertriglyceridemia in patients with metabolic syndrome, in ethnic groups, this combination is more common in indigenous rural men (53.3 %) and in non-indigenous urban women (54.3 %).","PeriodicalId":24058,"journal":{"name":"Сибирский научный медицинский журнал","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139533911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Semichev, E. Gereng, Т. N. Chekisheva, M. A. Kyazimova
A clinical case of the development of tubular-villous adenoma with moderate epithelial dysplasia (high grade) is described, taking into account the presence of foci of squamous metaplasia in the epithelium of the glands. The pathology presented in the article according to ICD-O has code 8263/2.Description of the clinical case. The patient had an initial colonoscopy in 2020 and was diagnosed with chronic ileitis and colitis with moderate and in some areas high inflammation activity, which is most characteristic of nonspecific ulcerative colitis. The patient was treated by a gastroenterologist. In December 2021, she had a follow-up colonoscopy with the histological material sampling. Endoscopic conclusion: ulcerative colitis, total lesion, exacerbation phase.Results and discussion. When the device was inserted into the rectum at a distance of 13 cm, a neoplasm was visualized, the mucosa was pale, shiny, when trying to pass the device through the narrowing of the contact-vulnerable mucosa, with instrumental palpation, the formation was displaced by a conglomerate, a polyfragmentary biopsy was taken. In the study of histological preparations of the recto-sigmoid junction, a tubular-villous adenoma with moderate epithelial dysplasia (high grade) is determined, taking into account the presence of foci of squamous metaplasia in the epithelium of the glands. According to the literature, with ulcerative colitis affecting almost the entire colon for more than 10 years, there is an increased risk of colon cancer formation (10 %), which increases by 2–5 % annually. The possibility of malignancy is present in 10–20 % of patients with ulcerative colitis for more than 20 years. Other researchers describe the identified low-grade dysplasia as progressing to high-grade dysplasia and carcinoma in 29–54 % of cases.Conclusions. The study of patients with this pathology should be carried out against the background of thorough preparation of the large intestine with modern preparations using high-tech verification methods and polyfragmental biopsy with a qualified assessment of biopsy specimens.
{"title":"Tubular-villous adenoma against the background of ulcerative colitis (clinical case)","authors":"E. Semichev, E. Gereng, Т. N. Chekisheva, M. A. Kyazimova","doi":"10.18699/ssmj20230628","DOIUrl":"https://doi.org/10.18699/ssmj20230628","url":null,"abstract":"A clinical case of the development of tubular-villous adenoma with moderate epithelial dysplasia (high grade) is described, taking into account the presence of foci of squamous metaplasia in the epithelium of the glands. The pathology presented in the article according to ICD-O has code 8263/2.Description of the clinical case. The patient had an initial colonoscopy in 2020 and was diagnosed with chronic ileitis and colitis with moderate and in some areas high inflammation activity, which is most characteristic of nonspecific ulcerative colitis. The patient was treated by a gastroenterologist. In December 2021, she had a follow-up colonoscopy with the histological material sampling. Endoscopic conclusion: ulcerative colitis, total lesion, exacerbation phase.Results and discussion. When the device was inserted into the rectum at a distance of 13 cm, a neoplasm was visualized, the mucosa was pale, shiny, when trying to pass the device through the narrowing of the contact-vulnerable mucosa, with instrumental palpation, the formation was displaced by a conglomerate, a polyfragmentary biopsy was taken. In the study of histological preparations of the recto-sigmoid junction, a tubular-villous adenoma with moderate epithelial dysplasia (high grade) is determined, taking into account the presence of foci of squamous metaplasia in the epithelium of the glands. According to the literature, with ulcerative colitis affecting almost the entire colon for more than 10 years, there is an increased risk of colon cancer formation (10 %), which increases by 2–5 % annually. The possibility of malignancy is present in 10–20 % of patients with ulcerative colitis for more than 20 years. Other researchers describe the identified low-grade dysplasia as progressing to high-grade dysplasia and carcinoma in 29–54 % of cases.Conclusions. The study of patients with this pathology should be carried out against the background of thorough preparation of the large intestine with modern preparations using high-tech verification methods and polyfragmental biopsy with a qualified assessment of biopsy specimens.","PeriodicalId":24058,"journal":{"name":"Сибирский научный медицинский журнал","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139533711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. B. Strok, M. Chenkurov, M. Kostyleva, D. A. Umutkuzina
A clinical case of the co-medication of valproic acid and cilastatin/imipenem in a 17-year-old girl with generalized tonic-clonic seizures is described. As an antiepileptic therapy, the child was prescribed valproic acid, the concentration of which in the blood plasma was more than 80 µg/ml, and seizures were not observed during this therapy. At the same time, due to the developed pneumonia, the girl was imipenem/cilastatin prescribed. Co-medication of drugs led to a decrease in the concentration of valproic acid in the blood plasma less than 50 µg/ ml, relapse of epileptic seizures was noted. Correction of antiepileptic therapy with phenobarbital did not lead to the seizure control. The removal of imipenem/ cilastatin contributed to the increase in the concentration of valproic acid in blood plasma above 50 µg/ ml, which corresponds to the recommended therapeutic range. Within two weeks after discontinuation of imipenem/ cilastatin, the concentration of valproic acid reached values as close as possible to the values before the start of antibiotic therapy. The authors of the article concluded that it is necessary to conduct therapeutic drug monitoring in children receiving valproic acid and imipenem/ cilastatin (antibiotic of carbapenem group), based on the known mechanisms of pharmacokinetic interaction.
{"title":"Decrease in the effectiveness of valproic acid against the background of antibacterial therapy with imipenem/cilastatin in pediatric practice: A case report","authors":"A. B. Strok, M. Chenkurov, M. Kostyleva, D. A. Umutkuzina","doi":"10.18699/ssmj20230627","DOIUrl":"https://doi.org/10.18699/ssmj20230627","url":null,"abstract":"A clinical case of the co-medication of valproic acid and cilastatin/imipenem in a 17-year-old girl with generalized tonic-clonic seizures is described. As an antiepileptic therapy, the child was prescribed valproic acid, the concentration of which in the blood plasma was more than 80 µg/ml, and seizures were not observed during this therapy. At the same time, due to the developed pneumonia, the girl was imipenem/cilastatin prescribed. Co-medication of drugs led to a decrease in the concentration of valproic acid in the blood plasma less than 50 µg/ ml, relapse of epileptic seizures was noted. Correction of antiepileptic therapy with phenobarbital did not lead to the seizure control. The removal of imipenem/ cilastatin contributed to the increase in the concentration of valproic acid in blood plasma above 50 µg/ ml, which corresponds to the recommended therapeutic range. Within two weeks after discontinuation of imipenem/ cilastatin, the concentration of valproic acid reached values as close as possible to the values before the start of antibiotic therapy. The authors of the article concluded that it is necessary to conduct therapeutic drug monitoring in children receiving valproic acid and imipenem/ cilastatin (antibiotic of carbapenem group), based on the known mechanisms of pharmacokinetic interaction.","PeriodicalId":24058,"journal":{"name":"Сибирский научный медицинский журнал","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139534111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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