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JOURNAL OF THE BELARUSIAN STATE UNIVERSITY ECOLOGY最新文献

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DAMAGE CAUSED BY LARVAE OF MINING FLY (PHYTOMYZA AGROMYZINA MEIGEN, 1830) TO DOGWOODS IN URBAN GREEN SPACES 采蝇(phytomyza agromyzina meigen)幼虫对城市绿地山茱萸的危害
Pub Date : 1900-01-01 DOI: 10.46646/2521-683x/2021-2-15-20
M. V. Lazarenko, S. Buga
According to the results of the investigations, carried out in 2017–2020 in the green spaces of cities in Belarus, the damage caused by larvae of Phytomyza agromyzina Meigen, 1830 to Siberian dogwood (Cornus alba L.) and blood twig dogwood (Cornus sanguinea (L.) Opiz), based on determining the relative area of damaged leaf surface, was assessed. The values of this parameter for individual leaf blades varied in the range from 0.86 to 63.58 %, while the average for the samples did not exceed 24.47 %. The total area of Ph. agromyzina mines on individual Cornus leaf blades ranged from 0.23 to 3.65 cm2. Using the nonparametric Mann–Whitney and Kolmogorov–Smirnov tests, the differences in medians and the integral differences between the samples were estimated. These values were not statistically significant when comparing data for Minsk and Vitebsk, and different years for both C. alba and C. sanguinea. However, in the values of the relative area of the damaged surface between the samples of both Cornus species the differences were statistically significant (p <0.05).
根据2017-2020年在白俄罗斯城市绿地进行的调查结果,1830型绿僵菌(Phytomyza agromyzina Meigen, 1830)幼虫对西伯利亚山茱萸(Cornus alba L.)和血枝山茱萸(Cornus sanguinea (L.))的危害。在确定叶片表面受损相对面积的基础上,对其进行评价。该参数对单个叶片的取值范围为0.86 ~ 63.58%,而对样品的平均值不超过24.47%。山茱萸单个叶片上的Ph. agromyzina地雷总面积为0.23 ~ 3.65 cm2。使用非参数Mann-Whitney检验和Kolmogorov-Smirnov检验,估计样本之间的中位数差异和积分差异。当比较明斯克和维捷布斯克的数据以及不同年份的白桦和血桦数据时,这些值没有统计学意义。两种山茱萸样品的损伤面相对面积值差异有统计学意义(p <0.05)。
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引用次数: 0
PHOTOPROTECTIVE, CYTOTOXIC AND PHOTOMODIFYING ACTIVITY OF LICHEN BIOMASS NON-POLAR FRACTIONS AGAINST HUMAN KERATINOCYTE (HaCAT) 地衣生物质非极性组分对人角质细胞(HaCAT)的光保护、细胞毒和光电化活性
Pub Date : 1900-01-01 DOI: 10.46646/2521-683x/2021-2-29-35
Volga M. Khramchankova, M. V. Matveyenkau
In vitro, the photoprotective, cytotoxic and photomodifying properties of hexane and benzene extracts from the widely distributed in Belarus lichens Cladonia arbuscula, Evernia prunastri, Hypogymnia physodes, Ramalina pollinaria and Xanthoria parietina in the culture of human keratinocytes (HaCAT), are estimated. It was found that these extracts are not photoprotective, they have a photomodifying effect upon irradiation of keratinocyte cultures with ultraviolet light. Hexane extract of E. prunastri and benzene extracts of R. pollinaria and H. physodes at a concentration of 2.5 μg/ml are photoprotectors that weaken the effect of ultraviolet radiation by 1.6 ÷ 1.8 times. Extracts of C. arbuscula and X. parietina are photosensitizers – they increase the toxic effect of ultraviolet radiation by 10 or more times, regardless of the concentration of the extract in the nutrient medium. Hexane and benzene extracts of C. arbuscula and H. physodes lichens are cytotoxic to the HaCAT culture. With an increase in the dose of ultraviolet irradiation of keratinocytes from zero to lethal values, the benzene extract of R. pollinaria acted as a photoprotector at a concentration of 2.5 μg / ml, and as a photosensitizer at higher concentrations. The hexane and benzene extracts of E. prunastri and H. physodes exhibited sensitizing properties that increased with an increase in the concentration of lichen extracts. C. arbuscula hexane extract and X. parietina benzene extract were the most potent photosensitizers.
在体外,对广泛分布于白俄罗斯的地衣Cladonia arbuscula、Evernia prunastri、Hypogymnia physodes、Ramalina pollinaria和Xanthoria parietina的己烷和苯提取物在人角质形成细胞(HaCAT)培养中的光保护、细胞毒性和光修饰特性进行了评估。发现这些提取物不具有光保护作用,在紫外线照射角质细胞培养物时,它们具有光修饰作用。紫李己烷萃取物和花楸、花楸的苯萃取物浓度为2.5 μg/ml时具有光保护剂作用,可使紫外线的作用减弱1.6 ~ 1.8倍。丛树花和顶叶花的提取物是光敏剂——无论其在营养培养基中的浓度如何,它们都能使紫外线辐射的毒性作用增加10倍或更多。正己烷和苯提取物对HaCAT培养具有细胞毒性。随着紫外线照射角化细胞的剂量从0到致死值的增加,在2.5 μg / ml浓度下,传粉菊苯提取物具有光保护剂作用,在更高浓度下具有光敏剂作用。青苔提取物的正己烷和苯提取物的致敏性随青苔提取物浓度的增加而增加。竹节木己烷提取物和竹节木苯提取物是最有效的光敏剂。
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引用次数: 0
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JOURNAL OF THE BELARUSIAN STATE UNIVERSITY ECOLOGY
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