T. Truong, Trang T P Phan, Linh Le, D. Nguyen, H. Nguyen, D. T. Dang
Introduction: The formation of G-quadruplex plays a key role in many biological processes. Therefore, visualization of G-quadruplex is highly essential for design of G-quadruplex-targeted small molecules (drugs). Herein, we report on an engineered fluorescent protein probe which was able to distinguish G-quadruplex topologies. Methods: The fluorescent protein probe was generated by genetically incorporating yellow fluorescent protein (YFP) to RNA helicase associated with AU-rich element (RHAU) peptide motif. Results: This probe could selectively bind and visualize parallel G-quadruplex structure (T95-2T) at high affinity (Kd~130 nM). Visualization of the parallel G-quadruplex by RHAU-YFP could be easily observed in vitro by using normal Gel Doc or the naked eye. Conclusion: The YFP probe could be encoded in cells to provide a powerful tool for detection of parallel G-quadruplexes both in vitro and in vivo.
g -四重体的形成在许多生物过程中起着关键作用。因此，g -四聚体的可视化对于g -四聚体靶向小分子(药物)的设计至关重要。在此，我们报道了一种工程荧光蛋白探针，它能够区分g -四重体拓扑结构。方法:将黄色荧光蛋白(YFP)与富au元素(RHAU)肽基序相关的RNA解旋酶基因结合，制备荧光蛋白探针。结果:该探针能以高亲和力(Kd~130 nM)选择性结合并可视化平行g -四联体结构(T95-2T)。通过正常凝胶或肉眼观察，RHAU-YFP可以很容易地在体外观察到平行g -四联体的可视化。结论:YFP探针可在细胞内编码，为体外和体内平行g -四联体的检测提供了强有力的工具。
{"title":"Engineering yellow fluorescent protein probe for visualization of parallel DNA G-quadruplex","authors":"T. Truong, Trang T P Phan, Linh Le, D. Nguyen, H. Nguyen, D. T. Dang","doi":"10.32508/STDJ.V21I3.461","DOIUrl":"https://doi.org/10.32508/STDJ.V21I3.461","url":null,"abstract":"Introduction: The formation of G-quadruplex plays a key role in many biological processes. Therefore, visualization of G-quadruplex is highly essential for design of G-quadruplex-targeted small molecules (drugs). Herein, we report on an engineered fluorescent protein probe which was able to distinguish G-quadruplex topologies. \u0000Methods: The fluorescent protein probe was generated by genetically incorporating yellow fluorescent protein (YFP) to RNA helicase associated with AU-rich element (RHAU) peptide motif. \u0000 Results: This probe could selectively bind and visualize parallel G-quadruplex structure (T95-2T) at high affinity (Kd~130 nM). Visualization of the parallel G-quadruplex by RHAU-YFP could be easily observed in vitro by using normal Gel Doc or the naked eye. \u0000Conclusion: The YFP probe could be encoded in cells to provide a powerful tool for detection of parallel G-quadruplexes both in vitro and in vivo. \u0000 ","PeriodicalId":285953,"journal":{"name":"Science and Technology Development Journal","volume":"583 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123414327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nguyen Dien Quoc Bao, Le Hoang Chien, C. Tao, Trịnh Hoa Lăng
Elastic 12C+12C angular distributions at three bombarding energies of 102.1, 112.0 and 126.1 MeV were analyzed in the framework of optical model (OM) and compared to the experimental data. The reality of the OM analysis using the double folding potential depends on the chosen nuclear density distributions. In this work, we use two available models of nuclear density distributions obtained from the electron scattering experiments and the density functional theory (DFT). The OM results show that the former gives better description of the 12C nuclear density distribution than the latter. Therefore, the DFT should be worked on for improving the nuclear density description of 12C in the future.
{"title":"Analysis of 12C+12C scattering using different nuclear density distributions","authors":"Nguyen Dien Quoc Bao, Le Hoang Chien, C. Tao, Trịnh Hoa Lăng","doi":"10.32508/stdj.v21i3.431","DOIUrl":"https://doi.org/10.32508/stdj.v21i3.431","url":null,"abstract":"Elastic 12C+12C angular distributions at three bombarding energies of 102.1, 112.0 and 126.1 MeV were analyzed in the framework of optical model (OM) and compared to the experimental data. The reality of the OM analysis using the double folding potential depends on the chosen nuclear density distributions. In this work, we use two available models of nuclear density distributions obtained from the electron scattering experiments and the density functional theory (DFT). The OM results show that the former gives better description of the 12C nuclear density distribution than the latter. Therefore, the DFT should be worked on for improving the nuclear density description of 12C in the future.","PeriodicalId":285953,"journal":{"name":"Science and Technology Development Journal","volume":"34 5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134346219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A significant contribution to natural exposure of humans is radon gas, which emanates from the soil and may concentrate in dwellings. The level of radon exposure varies around the globe, but limited data are available on the daily variations of indoor radon concentrations. In this study, indoor radon measurements were performed continuously within one week at six different places in Dalat, Vietnam using the real time Smart Radon Detector Radon Eye+. The indoor radon behavior in a day follows a sine pattern, with peak values in the early morning and lowest values in the late afternoon. There are also some fluctuations at specific times due to different weather conditions. Indoor radon concentrations in the Dalat regions were found to exceed the recommended guidelines and thresholds; excessive radon levels warranting health concern were found (150.7-340.0 Bq/m3). Some corrective actions to reduce indoor radon concentrations were recommended. Annual effective doses on different age categories were also calculated.
{"title":"Measurement of Indoor Radon Concentration in Dalat area","authors":"H. Le","doi":"10.32508/STDJ.V21I2.432","DOIUrl":"https://doi.org/10.32508/STDJ.V21I2.432","url":null,"abstract":"A significant contribution to natural exposure of humans is radon gas, which emanates from the soil and may concentrate in dwellings. The level of radon exposure varies around the globe, but limited data are available on the daily variations of indoor radon concentrations. In this study, indoor radon measurements were performed continuously within one week at six different places in Dalat, Vietnam using the real time Smart Radon Detector Radon Eye+. The indoor radon behavior in a day follows a sine pattern, with peak values in the early morning and lowest values in the late afternoon. There are also some fluctuations at specific times due to different weather conditions. Indoor radon concentrations in the Dalat regions were found to exceed the recommended guidelines and thresholds; excessive radon levels warranting health concern were found (150.7-340.0 Bq/m3). Some corrective actions to reduce indoor radon concentrations were recommended. Annual effective doses on different age categories were also calculated.","PeriodicalId":285953,"journal":{"name":"Science and Technology Development Journal","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127936307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Striped catfish (Pangasianodon hypohthalmus) is a native freshwater fish species in the Mekong Delta, Vietnam, and significantly contributes to national aqua exports. Currently, however, the sustainable development of striped catfish farming is being affected by bacterial pathogen infections, of which hemorrhagic septicemia caused by Aeromonas hydrophila bacteria is one of the most common diseases. Methods: In this study, the stability of TG25P and CT45P phages to factors such as temperature, pH, and organic solvents was investigated, with the stability of TG25P being found to be higher than that of CT45P. Results: The activity of TG25P was retained to approximately 90% and 80% at 37oC and 50oC for 1 h, respectively. Its activity was maintained to greater than 80% at pH 5-9 for 24 h and approximately 90-100% in organic solvents, such as chloroform or diethyl ether, for 1 h. In addition, the stability and activity of TG25P for the control of A. hydrophila in striped catfish pond water was also evaluated for 48 h. Conclusion: TG25P was found to be highly applicable in the creation of low-cost phage-containing products for the prospective application of phage therapy in prevention and treatment of hemorrhagic septicemia in striped catfish.
{"title":"Stability and activity of TG25P phage in control of Aeromonas hydrophila in striped catfish pond water","authors":"T. Xuân, H. A. Hoang, L. Tam","doi":"10.32508/STDJ.V21I2.429","DOIUrl":"https://doi.org/10.32508/STDJ.V21I2.429","url":null,"abstract":"Introduction: Striped catfish (Pangasianodon hypohthalmus) is a native freshwater fish species in the Mekong Delta, Vietnam, and significantly contributes to national aqua exports. Currently, however, the sustainable development of striped catfish farming is being affected by bacterial pathogen infections, of which hemorrhagic septicemia caused by Aeromonas hydrophila bacteria is one of the most common diseases. \u0000Methods: In this study, the stability of TG25P and CT45P phages to factors such as temperature, pH, and organic solvents was investigated, with the stability of TG25P being found to be higher than that of CT45P. \u0000Results: The activity of TG25P was retained to approximately 90% and 80% at 37oC and 50oC for 1 h, respectively. Its activity was maintained to greater than 80% at pH 5-9 for 24 h and approximately 90-100% in organic solvents, such as chloroform or diethyl ether, for 1 h. In addition, the stability and activity of TG25P for the control of A. hydrophila in striped catfish pond water was also evaluated for 48 h. \u0000Conclusion: TG25P was found to be highly applicable in the creation of low-cost phage-containing products for the prospective application of phage therapy in prevention and treatment of hemorrhagic septicemia in striped catfish. \u0000 ","PeriodicalId":285953,"journal":{"name":"Science and Technology Development Journal","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126674885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Recognizing human activity in a daily environment has attracted much research in computer vision and recognition in recent years. It is a difficult and challenging topic not only inasmuch as the variations of background clutter, occlusion or intra-class variation in image sequences but also inasmuch as complex patterns of activity are created by interactions among people-people or people-objects. In addition, it also is very valuable for many practical applications, such as smart home, gaming, health care, human-computer interaction and robotics. Now, we are living in the beginning age of the industrial revolution 4.0 where intelligent systems have become the most important subject, as reflected in the research and industrial communities. There has been emerging advances in 3D cameras, such as Microsoft's Kinect and Intel's RealSense, which can capture RGB, depth and skeleton in real time. This creates a new opportunity to increase the capabilities of recognizing the human activity in the daily environment. In this research, we propose a novel approach of daily activity recognition and hypothesize that the performance of the system can be promoted by combining multimodal features. Methods: We extract spatial-temporal feature for the human body with representation of parts based on skeleton data from RGB-D data. Then, we combine multiple features from the two sources to yield the robust features for activity representation. Finally, we use the Multiple Kernel Learning algorithm to fuse multiple features to identify the activity label for each video. To show generalizability, the proposed framework has been tested on two challenging datasets by cross-validation scheme. Results: The experimental results show a good outcome on both CAD120 and MSR-Daily Activity 3D datasets with 94.16% and 95.31% in accuracy, respectively. Conclusion: These results prove our proposed methods are effective and feasible for activity recognition system in the daily environment.
{"title":"Multiple Modal Features and Multiple Kernel Learning for Human Daily Activity Recognition","authors":"V. Vo, Hoang M Pham","doi":"10.32508/STDJ.V21I2.441","DOIUrl":"https://doi.org/10.32508/STDJ.V21I2.441","url":null,"abstract":"Introduction: Recognizing human activity in a daily environment has attracted much research in computer vision and recognition in recent years. It is a difficult and challenging topic not only inasmuch as the variations of background clutter, occlusion or intra-class variation in image sequences but also inasmuch as complex patterns of activity are created by interactions among people-people or people-objects. In addition, it also is very valuable for many practical applications, such as smart home, gaming, health care, human-computer interaction and robotics. Now, we are living in the beginning age of the industrial revolution 4.0 where intelligent systems have become the most important subject, as reflected in the research and industrial communities. There has been emerging advances in 3D cameras, such as Microsoft's Kinect and Intel's RealSense, which can capture RGB, depth and skeleton in real time. This creates a new opportunity to increase the capabilities of recognizing the human activity in the daily environment. In this research, we propose a novel approach of daily activity recognition and hypothesize that the performance of the system can be promoted by combining multimodal features. \u0000Methods: We extract spatial-temporal feature for the human body with representation of parts based on skeleton data from RGB-D data. Then, we combine multiple features from the two sources to yield the robust features for activity representation. Finally, we use the Multiple Kernel Learning algorithm to fuse multiple features to identify the activity label for each video. To show generalizability, the proposed framework has been tested on two challenging datasets by cross-validation scheme. \u0000Results: The experimental results show a good outcome on both CAD120 and MSR-Daily Activity 3D datasets with 94.16% and 95.31% in accuracy, respectively. \u0000Conclusion: These results prove our proposed methods are effective and feasible for activity recognition system in the daily environment. \u0000 ","PeriodicalId":285953,"journal":{"name":"Science and Technology Development Journal","volume":"31 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131745659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In Viet Nam, data from Conference of Cancer organized by the Ministry of Health has shown that breast cancer is the most popular cancer in women. Current mainly treatments are surgery, chemotherapy, and radiotherapy. However, the rate of recurrence after five years was very high. One of the causes of high relapse is cancer cells develop multidrug-resistant (MDR) thus reduced the efficiency of treatments. In this research, MTT assay was used for measured cell viability of Vietnamese breast cancer cells (VNBRCA cells) and positive control MCF-7 cell lines after treatment with several anticancer drugs as Doxorubicin (DOX), Tamoxifen (TAM), Mitomycin C (MMC) in 48h. After that, cancer cells were treated at haft maximal inhibitory concentration (IC50) of anticancer drug and observed cell morphology, apoptosis of cellular nuclear by AO/PI staining. IC50 value of VNBRCA cells with DOX, TAM, MMC were 0.641± 0.07 µM, 4.639 ± 0.933 µM and 1.338 ± 0.176 µM, respectively, which higher than IC50 of MCF-7 with DOX, TAM, MMC was 0.168 ± 0.037 µM, 7.085 ± 0.87 µM and 0.379 ± 0.159 µM, respectively. The response of VNBRCA cells with several anticancer drugs as DOX, TAM, and MMC was lower than the response of MCF-7, therefore, it showed that the specific features of VNBRCA cells; from which develop specific treatments for Vietnamese breast cancer patients.
{"title":"Evaluation the effect of several anticancer drugs on Vietnamese breast cancer cells","authors":"O. Nguyen","doi":"10.32508/stdj.v21i2.488","DOIUrl":"https://doi.org/10.32508/stdj.v21i2.488","url":null,"abstract":"In Viet Nam, data from Conference of Cancer organized by the Ministry of Health has shown that breast cancer is the most popular cancer in women. Current mainly treatments are surgery, chemotherapy, and radiotherapy. However, the rate of recurrence after five years was very high. One of the causes of high relapse is cancer cells develop multidrug-resistant (MDR) thus reduced the efficiency of treatments. In this research, MTT assay was used for measured cell viability of Vietnamese breast cancer cells (VNBRCA cells) and positive control MCF-7 cell lines after treatment with several anticancer drugs as Doxorubicin (DOX), Tamoxifen (TAM), Mitomycin C (MMC) in 48h. After that, cancer cells were treated at haft maximal inhibitory concentration (IC50) of anticancer drug and observed cell morphology, apoptosis of cellular nuclear by AO/PI staining. IC50 value of VNBRCA cells with DOX, TAM, MMC were 0.641± 0.07 µM, 4.639 ± 0.933 µM and 1.338 ± 0.176 µM, respectively, which higher than IC50 of MCF-7 with DOX, TAM, MMC was 0.168 ± 0.037 µM, 7.085 ± 0.87 µM and 0.379 ± 0.159 µM, respectively. The response of VNBRCA cells with several anticancer drugs as DOX, TAM, and MMC was lower than the response of MCF-7, therefore, it showed that the specific features of VNBRCA cells; from which develop specific treatments for Vietnamese breast cancer patients.","PeriodicalId":285953,"journal":{"name":"Science and Technology Development Journal","volume":"185 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122068894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper employs qualitative and quantitative methods to test the theory of Philips Curve in Vietnam in the period between 2000 and 2014. The results show that the Philips Curve applies to the actual situation of the Vietnam’s economy, which is useful for both macro-economic planning by the Government and monetary policy making by the State Bank of Vietnam. The paper also suggests implications of an increased application of the Philips Curve to the economic policy management, thereby contributing to the stabilized socio-economic development in Vietnam
{"title":"Testing the Phillips curve in Vietnam","authors":"Dandan Dang, Binh Vu","doi":"10.32508/stdj.v19i1.527","DOIUrl":"https://doi.org/10.32508/stdj.v19i1.527","url":null,"abstract":"This paper employs qualitative and quantitative methods to test the theory of Philips Curve in Vietnam in the period between 2000 and 2014. The results show that the Philips Curve applies to the actual situation of the Vietnam’s economy, which is useful for both macro-economic planning by the Government and monetary policy making by the State Bank of Vietnam. The paper also suggests implications of an increased application of the Philips Curve to the economic policy management, thereby contributing to the stabilized socio-economic development in Vietnam","PeriodicalId":285953,"journal":{"name":"Science and Technology Development Journal","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134016335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A HPLC-DAD method for the determination of cysteamine supplementation in commercial animal feeds was developed. Samples were extracted with a mixture of 0.5 % hydrochloric acid – acetonitrile (90:10, v/v), matrix interferences were removed with a C18 cartridge, cysteamine was derivatized using 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB) as Ellman's reagent targeting to the thiol group in the molecule. Quantification of cysteamine was performed on a c18 column with DAD at 323 nm. The developed method had LOD of 1.1 mg/l, good linearity of the calibration curve (R2 ≥ 0.9998), high recoveries (> 92 %), and high reproducibility (RSD < 2.0%).
{"title":"Determination of cysteamine in animal feeds by high performance liquid chromatography with diode-array detection (HPLC-DAD)","authors":"P. H. Nguyen","doi":"10.32508/STDJ.V21I2.427","DOIUrl":"https://doi.org/10.32508/STDJ.V21I2.427","url":null,"abstract":"A HPLC-DAD method for the determination of cysteamine supplementation in commercial animal feeds was developed. Samples were extracted with a mixture of 0.5 % hydrochloric acid – acetonitrile (90:10, v/v), matrix interferences were removed with a C18 cartridge, cysteamine was derivatized using 5,5'-dithiobis-(2-nitrobenzoic) acid (DTNB) as Ellman's reagent targeting to the thiol group in the molecule. Quantification of cysteamine was performed on a c18 column with DAD at 323 nm. The developed method had LOD of 1.1 mg/l, good linearity of the calibration curve (R2 ≥ 0.9998), high recoveries (> 92 %), and high reproducibility (RSD < 2.0%).","PeriodicalId":285953,"journal":{"name":"Science and Technology Development Journal","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131791806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Water is one of the most important resources on Earth because of its vital role for human beings and other living creatures. During the devastating drought in the Mekong Delta in 2016, millions of people had no access to the secure source of fresh water. This issue cautioned and influenced the Vietnamese government to find an effective desalination process for the coastal areas impacted heavily by salinization. However, desalination processes require large amounts of energy which can be a problem for the coastal areas due to the lack of energy and high cost. The main purpose of the study herein was to find an alternative solution for desalination by designing a desalination system using solar energy. The design was expected to provide a small size desalination system for households. The average output of the system was about 854 ml/day with the productivity at 2223.54 ml/day/m2. The average water capture efficiency was 80.5%, the salt elimination efficiency of the system was about 99.99%, and the salinity of the output water was below 2 ppm. The results are still far from ideal but they indicate a potential solution for coping with the shortage of fresh water in coastal areas.
{"title":"Research and design of an improved solar still for desalination","authors":"Truong Thi Cam Trang, Dinh Pham, P. T. Phan","doi":"10.32508/STDJ.V21I2.423","DOIUrl":"https://doi.org/10.32508/STDJ.V21I2.423","url":null,"abstract":"Water is one of the most important resources on Earth because of its vital role for human beings and other living creatures. During the devastating drought in the Mekong Delta in 2016, millions of people had no access to the secure source of fresh water. This issue cautioned and influenced the Vietnamese government to find an effective desalination process for the coastal areas impacted heavily by salinization. However, desalination processes require large amounts of energy which can be a problem for the coastal areas due to the lack of energy and high cost. The main purpose of the study herein was to find an alternative solution for desalination by designing a desalination system using solar energy. The design was expected to provide a small size desalination system for households. The average output of the system was about 854 ml/day with the productivity at 2223.54 ml/day/m2. The average water capture efficiency was 80.5%, the salt elimination efficiency of the system was about 99.99%, and the salinity of the output water was below 2 ppm. The results are still far from ideal but they indicate a potential solution for coping with the shortage of fresh water in coastal areas.","PeriodicalId":285953,"journal":{"name":"Science and Technology Development Journal","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123643084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Human adipose derived stem cells (hASCs) have great potential for regenerative medicine. The demand for hASCs, especially in the development of off-the-shelf products, is increasing. Although the initial receipt of hASCs was relatively limited, there is now greater interest and also awareness that in vitro expansion of hASCs be further explored. The purpose of this study was to assess the integrity of mesenchymal cell characteristics and the mutant capability of chromosome number on hASCs undergoing in vitro expansion. Methods: In this study, three hASC samples from three Vietnamese people were collected and proliferated in MSCCult medium (Regemedlab, Ho Chi Minh City, Viet Nam) to the 5th cell passage. Next, hASCs were evaluated for change of mesenchymal stem cells (MSCs) characteristics including shape, immunophenotype (CD14, CD34, CD44, CD73, CD90, and/or CD166), and trilineage differentiation ability. Finally, the number of chromosomes after passages 1, 3, and 5 was evaluated by karyotyping technique. Results: The results showed that after five passages of culture, hASCs preserved the characteristic shape of MSCs, high expression of mesenchymal markers (e.g. CD44, CD73, CD90, and CD166). However, the cells also maintained their differentiation capacity to develop into various tissues such as bone, cartilage, and fat. The hASCs showed no mutation in the number of chromosomes. However, markers of hematopoietic cells (such as CD14 and CD34) exhibited heterogeneous changes between the samples during proliferation. Conclusion: In conclusion, at passage 5, hASCs retained the integrity of MSC features and there was no mutation discovered in the number of chromosomes. However, further evaluation is needed to conclude that the use of cultured cells in treatment is effective and safe.
摘要:人脂肪源性干细胞(hASCs)具有巨大的再生医学潜力。对hasc的需求正在增加，特别是在开发现成产品方面。虽然最初接受的hASCs相对有限，但现在有更大的兴趣和意识到hASCs的体外扩增有待进一步探索。本研究的目的是评估间充质细胞特征的完整性和染色体数目在体外扩增的hASCs上的突变能力。方法:本研究收集3名越南人的3份hASC样本，在MSCCult培养基(Regemedlab, Ho Chi Minh City, vietnam)中增殖至第5代。接下来，评估hASCs间充质干细胞(MSCs)特征的变化，包括形状、免疫表型(CD14、CD34、CD44、CD73、CD90和/或CD166)和三岁分化能力。最后，用核型技术对1、3、5代后的染色体数目进行分析。结果:经过5代培养，hASCs保持了MSCs的特征形态，间充质标记物(CD44、CD73、CD90、CD166)的高表达。然而，细胞也保持了分化能力，发育成骨、软骨、脂肪等多种组织。hASCs在染色体数量上未见突变。然而，造血细胞的标志物(如CD14和CD34)在增殖过程中在样品之间表现出异质变化。结论:综上所述，在传代5时，hASCs保持了MSC特征的完整性，染色体数量未发生突变。然而，需要进一步的评估才能得出使用培养细胞治疗是有效和安全的结论。
{"title":"Phenotypic and cytogenetic characterization of expanded adipose derived stem cells","authors":"N. Truong, A. Vu, Vuong M Pham","doi":"10.32508/STDJ.V21I1.426","DOIUrl":"https://doi.org/10.32508/STDJ.V21I1.426","url":null,"abstract":"Introduction: Human adipose derived stem cells (hASCs) have great potential for regenerative medicine. The demand for hASCs, especially in the development of off-the-shelf products, is increasing. Although the initial receipt of hASCs was relatively limited, there is now greater interest and also awareness that in vitro expansion of hASCs be further explored. The purpose of this study was to assess the integrity of mesenchymal cell characteristics and the mutant capability of chromosome number on hASCs undergoing in vitro expansion. Methods: In this study, three hASC samples from three Vietnamese people were collected and proliferated in MSCCult medium (Regemedlab, Ho Chi Minh City, Viet Nam) to the 5th cell passage. Next, hASCs were evaluated for change of mesenchymal stem cells (MSCs) characteristics including shape, immunophenotype (CD14, CD34, CD44, CD73, CD90, and/or CD166), and trilineage differentiation ability. Finally, the number of chromosomes after passages 1, 3, and 5 was evaluated by karyotyping technique. Results: The results showed that after five passages of culture, hASCs preserved the characteristic shape of MSCs, high expression of mesenchymal markers (e.g. CD44, CD73, CD90, and CD166). However, the cells also maintained their differentiation capacity to develop into various tissues such as bone, cartilage, and fat. The hASCs showed no mutation in the number of chromosomes. However, markers of hematopoietic cells (such as CD14 and CD34) exhibited heterogeneous changes between the samples during proliferation. Conclusion: In conclusion, at passage 5, hASCs retained the integrity of MSC features and there was no mutation discovered in the number of chromosomes. However, further evaluation is needed to conclude that the use of cultured cells in treatment is effective and safe.","PeriodicalId":285953,"journal":{"name":"Science and Technology Development Journal","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116098933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：