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Production and quality of zucchini seeds after applying sulphur as top dressing and organic compost at planting 施硫追肥和有机堆肥对西葫芦种子产量和品质的影响
Pub Date : 2020-07-20 DOI: 10.21475/ajcs.20.14.07.p2049
E. M. Bardiviesso, Natália de Brito Lima Lanna, A. S. Aguilar, S. Bezerra, R. A. Pelvine, P. G. Freitas, Francisca Karla Kelly Da Silva, Marcela Sant’anna Cordeiro da Silva, A. Cardoso
The recommendation of fertilization for zucchini (Cucurbita pepo) cultivation is usually made with no differentiation from the other cucurbit crops. Thus, there is a need to examine the doses of fertilizers used for zucchini production, as well as the suitability for cultivars, seasons, and cultivation regions. The aim of this study was to evaluate the influence of sulfur doses at topdressing fertilization and the supply of organic compost at planting on the production and quality of zucchini seeds. A total of eight treatments were studied in a randomized block design with a factorial scheme 4x2, corresponding to four sulfur doses at topdressing (0, 57, 114 and 173 kg ha-1 of S), in the presence (30 t ha-1) and absence of organic compost at planting. The fruits were harvested 62 DAT, and after harvest, the fruits remained in rest for 15 days in order to reach the uniformity of the physiological maturity of the seeds. The production characteristics evaluated were average fresh weight, diameter, dry weight and length of the fruits, fruit yield per plant, weight of a thousand seeds, number and seed weight. Regarding the seed quality, the percentage of germination, germination speed index, first count of the germination test, and electric conductivity, were evaluated. The supply of organic compost at planting had a positive effect on the production and physiological quality of the seeds of zucchini produced. The sulfur applied at topdressing improved the presence of the organic compost and also the production of seeds. Hence, it is recommended to apply 30 t ha-1 of organic compost at planting in association with 57 kg ha-1 of S at topdressing for higher seed yield and quality.
西葫芦(Cucurbita pepo)栽培的施肥建议通常与其他葫芦作物没有区别。因此,有必要检查西葫芦生产中使用的肥料剂量,以及对品种、季节和栽培区域的适用性。本研究旨在评价追肥时硫用量和种植时有机肥用量对西葫芦种子产量和品质的影响。采用随机区组设计,采用4x2析因方案,对8个处理进行了研究,分别为施肥时施硫量为0、57、114和173 kg hm -1,种植时施硫量为30 t hm -1和不施有机堆肥。果实收获62天,收获后静置15天,以达到种子生理成熟的均匀性。评价的生产性状为果实的平均鲜重、直径、干重和长度、单株产量、千粒重、数量和种子重。对种子质量进行发芽率、发芽速度指数、发芽试验第一次计数、电导率等评价。种植时施用有机堆肥对西葫芦产量和种子生理品质有积极影响。追肥时施用的硫改善了有机堆肥的存在,也提高了种子的产量。因此,建议在播种时施用30吨/公顷的有机堆肥,并在追肥时施用57公斤/公顷的S,以提高种子产量和质量。
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引用次数: 1
Stalk dry mass and industrial yield of 16 varieties of sugar cane cultivated under water restriction 限水栽培16个甘蔗品种茎秆干质量及工业产量
Pub Date : 2020-07-20 DOI: 10.21475/ajcs.20.14.07.p1899
J. C. R. Anjos, D. Casaroli, J. A. Junior, A. W. P. Evangelista, R. Battisti, M. Mesquita
The great extension of the cultivated area, associated with the low water availability to supply the sugarcane demand in the periods of drought and the high evapotranspiration demand, requires varieties adapted to these specific conditions. The aim of this study was to assess 16 sugarcane varieties regarding the efficiency in water use (EWU) and in photosynthetically active radiation (PAR), cultivated under a water restriction, in the production of stalks dry matter, sugar and alcohol. The experiment was carried out in the Brazilian savannah biome, Goiás State, during the 2011/2012 crop year, in a randomized blocks design, with four repetitions. The treatments consisted of 16 varieties of sugarcane cultivated on a supply of only 50% of the water demand demanded by the crop. The EWU and EUPAR of the varieties were evaluated for the production of stalk dry mass (SDM), sugar and alcohol. During the crop cycle there was sufficient precipitation to supply the water demand of sugarcane; however, the irregular distribution of rainfall resulted in a water deficit of -697 mm during its cycle. The varieties IAC 91-1099, CTC-15, CTC-11, SP 86-0042 and IAC 87-3396 showed higher EWU and better photosynthetically active radiation for the production of stalks dry matter, sugar and alcohol. IACSP 94-2094 and CTC 09 varieties presented the same efficiency in industrial yield and lower dry matter yield than the five following varieties IAC 91-1099, CTC-15, CTC-11, SP 86-0042 and IAC 87-3396. Therefore, these last varieties are the most efficient in water use and photosynthetically active radiation aiming the stalks dry matter, sugar and alcohol production under water restriction.
耕地面积的扩大,与干旱时期满足甘蔗需求的低水分供应和高蒸散需求有关,需要适应这些特定条件的品种。本研究的目的是评价16个甘蔗品种在限水栽培条件下的水分利用效率(EWU)和光合有效辐射效率(PAR),以及茎干物质、糖和酒精的生产效率。该实验于2011/2012作物年度在巴西热带草原生物群系Goiás州进行,采用随机区组设计,重复4次。这些处理包括16个品种的甘蔗,在只有作物需水量50%的情况下种植。评价了各品种茎秆干质量(SDM)、糖和酒精的EWU和EUPAR。在作物周期,有足够的降水来满足甘蔗的需水量;然而,降雨分布的不均匀性导致其在周期内亏水量为-697 mm。品种IAC 91-1099、CTC-15、CTC-11、SP 86-0042和IAC 87-3396在茎干物质、糖和酒精生产方面表现出较高的EWU和较好的光合有效辐射。与IAC 91-1099、CTC-15、CTC-11、SP 86-0042和IAC 87-3396相比,IACSP 94-2094和CTC 09的工业产量效率相同,干物质产量较低。因此,在水分限制条件下,这些品种在茎秆干物质、糖和酒精产量方面的水分利用和光合有效辐射效率最高。
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引用次数: 2
Chemical and microbiological evaluations of Platonia insignis Mart and Theobroma grandiflorum Schum pulps, native fruits from Brazilian Amazon 巴西亚马逊地区原产水果桔梗和桔梗果肉的化学和微生物学评价
Pub Date : 2020-07-20 DOI: 10.21475/ajcs.20.14.07.p2559
Patrycia Elen Costa Amorim, J. H. R. Araujo, Aline Priscilla Gomes da Silva, Jéssica dos Santos Almeida, M. O. Arruda, I. M. R. S. Serra, E. U. Alves, P. A. F. R. Melo
The aim of the present study was to evaluate chemical and microbiological parameters of industrialized and artisanal bacuri (Platonia insignis Mart) and cupuaçu (Theobroma grandiflorum Schum) pulps, native Brazilian Amazon fruits. Several fruit pulp brands were selected as follows: industrialized pulps (IP), brands A and B and artisanal pulps (AP), brands C and D. The chemical analyses were based on the determination of initial pH, total titratable acidity (TTA), total soluble solids (TSS) and their ratio (TSS/TTA). Microbiological analyzes quantified mold, yeast, Staphyloccus aureus and Salmonella spp. Their contamination were also determined. Chemical characterization demonstrated that bacuri pulps had lower level of TTA and TSS than cupuaçu pulp. However, ration between TSS and TAA showed higher level in bacuri pulp/brand AP/D. The microbiological analyses showed a ranging from 2.83x105 to 9.35x107 and 7.32x106 to 7.06x107 mold and yeast colony forming units (CFU/g) for bacuri and cupuaçu pulps, respectively. All pulps presented high amounts of Staphylococcus aureus. The presence of Salmonella was verified in industrialized pulp for both bacuri and cupuaçu fruits. We concluded that both industrialized and artisanal bacuri and cupuaçu pulps have poor microbiological food quality, indicating a health threat to the consumers.
本研究的目的是评价工业和手工生产的巴西亚马逊原生水果巴库利(Platonia insignis Mart)和cupuau (Theobroma citiflorum Schum)果肉的化学和微生物参数。选择了工业纸浆(IP) A、B品牌和手工纸浆(AP) C、d品牌。化学分析基于初始pH、总可滴定酸度(TTA)、总可溶性固形物(TSS)及其比值(TSS/TTA)的测定。微生物学分析对霉菌、酵母菌、金黄色葡萄球菌和沙门氏菌进行了定量分析,并对其污染情况进行了测定。化学表征表明,杆状藻浆的TTA和TSS含量低于cupuapuru浆。而TSS与TAA的比值在杆状菌浆/品牌AP/D中较高。微生物学分析结果表明,杆状菌浆的霉菌和酵母菌菌落形成单位(CFU/g)分别为2.83 × 105 ~ 9.35 × 107和7.32 × 106 ~ 7.06 × 107。所有纸浆均含有大量金黄色葡萄球菌。对巴库利果和瓜帕拉瓜果的工业化果肉中沙门氏菌的存在进行了验证。我们得出结论,无论是工业化还是手工生产的巴库里果肉和瓜帕拉瓜果肉,其微生物食品质量都很差,对消费者的健康构成威胁。
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引用次数: 1
An econometric model for demand of pulpwood market in Brazil 巴西纸浆木材市场需求的计量经济模型
Pub Date : 2020-07-20 DOI: 10.21475/ajcs.20.14.07.p2244
H. Angelo, Tomas V. Angelo, A. N. D. Almeida, P. G. A. Vasconcelos, M. Magliano, A. A. Brasil, Á. N. Souza, E. P. Miguel
The pulp industry has a great importance to the economy of Brazil and despite of being one of the biggest producer in the world this industry is still expanding in the country. In spite of the importance of the planted forests as main source for the pulp industry and other products, pulpwood for the cellulose market has received little attention in Brazil. Therefore, the objective of this research is the study of pulpwood demand in Brazil from 1994 to 2016, using econometric tools, where the demand equation was specified and adjusted by the Ordinary Least Squares method. The results showed that pulpwood price and the capacity of the pulp industry explain the pulpwood demand with good econometric results. The pulpwood demand is inelastic to price fluctuations and elastic to installed capacity. These results are consistent with the international estimations and they can assist projecting policies that promote more rational and sustainable management of the wood and consequently the forests.
纸浆工业对巴西的经济非常重要,尽管巴西是世界上最大的纸浆生产国之一,但纸浆工业在巴西仍在扩大。尽管人工林作为纸浆工业和其他产品的主要来源具有重要意义,但用于纤维素市场的纸浆木材在巴西很少受到关注。因此,本研究的目的是研究1994年至2016年巴西纸浆木材需求,使用计量经济学工具,其中需求方程被指定并通过普通最小二乘法进行调整。结果表明,纸浆价格和纸浆工业产能能够很好地解释纸浆需求。纸浆木材需求对价格波动无弹性,对装机容量有弹性。这些结果与国际估计一致,它们可以帮助预测促进更合理和可持续的木材管理的政策,从而促进森林。
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引用次数: 0
Effect of reduced spacing on relationship of physiological, morphological and productive traits of corn yield 缩短间距对玉米生理、形态及生产性状关系的影响
Pub Date : 2020-07-20 DOI: 10.21475/ajcs.20.14.07.p2630
Luan de Oliveira Nascimento, J. Ferreira, G. O. Nascimento, V. B. Santos, Clemeson Silva de Souza, Antonia Fabiana Barros de Lima, Francisco Ian de Oliveira Nascimento
Increasing corn grain production without devastating new forest areas is a viable alternative to controlling deforestation. However, increasing plant density in the area may alter plant morphophysiological and productive traits. The objective of this study was to characterize relationships between physiological, morphological and yield traits of corn plants, as well as the cause, effect and relationship of the traits on grain yield. The experiment was carried out in randomized complete block design with four replications. The corn hybrids (2B655PW, AG7088PRO3 and P4285YHR) were grown with row spacing of 40 cm, 60 cm, 80 cm, 95 cm. The evaluated traits physiological were: net photosynthesis (PN), stomatal conductance (Gs), intercellular concentration of CO2 (Ci), leaf transpiration (E), water use efficiency (WUE) and carboxylation efficiency (CE). The morphological were: plant height (PH) and ear insertion height (EIH), stem diameter (SD), and leaf area (LA) and the productive traits were the total number of ear per hectare (NE), number of grains per ear (NGE), grain mass per ear (GME), 100 grain weight (100GW) and grain yield (GY). The characteristics of maize hybrids cultivated in environment with reduced spacing (40cm, 60cm, 80cm, 95cm) of the 2016/2017 crop were investigated through the multicollinearity path analysis. The physiological, morphological and productive traits are considered sources of variation of cause and effect of corn yield in reduced spaced. This traits are essential for observations in maize breeding programs to obtain high yielding varieties in reduced spacing. In conclusion, the physiological (PN, CE, WUE, Ci, Gs), morphological (EIH, SD, LA) and productive (NE, GME) traits provide gains in maize grain yield via indirect selection when the crop is subjected to 40 cm row spacing.
在不破坏新森林的情况下增加玉米产量是控制森林砍伐的可行替代方案。然而,增加该地区的植物密度可能会改变植物的形态生理和生产性状。本研究旨在探讨玉米植株生理、形态与产量性状之间的关系,以及这些性状与产量之间的因果关系和相互关系。试验采用随机完全区组设计,设4个重复。玉米杂交种2B655PW、AG7088PRO3和P4285YHR行距分别为40、60、80、95 cm。生理性状包括净光合作用(PN)、气孔导度(Gs)、细胞间CO2浓度(Ci)、叶片蒸腾(E)、水分利用效率(WUE)和羧化效率(CE)。形态性状为株高(PH)、穗高(EIH)、茎粗(SD)和叶面积(LA),生产性状为每公顷穗数(NE)、每穗粒数(NGE)、每穗粒质量(GME)、百粒重(100GW)和产量(GY)。采用多重共线性通径分析方法,研究了2016/2017年度玉米杂交种在40cm、60cm、80cm、95cm减距环境下的性状。生理、形态和生产性状被认为是减少间距玉米产量因果变化的根源。这一性状对于在玉米育种计划中观察以获得小间距的高产品种是必不可少的。综上所述,当作物行间距为40 cm时,生理性状(PN、CE、WUE、Ci、Gs)、形态性状(EIH、SD、LA)和生产性状(NE、GME)通过间接选择提高了玉米产量。
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引用次数: 0
Post-emergence application of glufosinate on maize hybrids containing the phosphinothricin acetyltransferase gene (pat) 草铵膦在含磷丙氨酸乙酰转移酶基因玉米杂交种上的苗期施用
Pub Date : 2020-07-20 DOI: 10.21475/ajcs.20.14.07.p2241
F. H. Krenchinski, C. A. Carbonari, E. Castro, D. M. Rodrigues, V. Cesco, R. N. Costa, E. D. Velini
Several maize hybrids that present the phosphinothricin acetyltransferase gene (pat) are available in the market. However, these hybrids have different resistance levels to glufosinate herbicides. The objective of the present work was to evaluate the resistance of maize hybrids containing the pat gene (as a selection marker) to glufosinate. Field experiments were conducted in two sites in the 2016/2017 crop season, using a randomized block design with a 2×7 factorial arrangement and four replications. The treatments consisted of two glufosinate rates (0 and 500 g ha-1) and seven maize hybrids, six containing the pat gene as a selection marker (Herculex®, Agrisure-TL®, Herculex Yieldgard®, Leptra®, Viptera-3®, and Power-Core®) and one without the pat gene (VT PRO®). Two field experiments were conducted in different sites. The analyzed variables were: ammonia accumulation, electron transport rate (ETR), percentage of injuries, 100-grain weight, and grain yield. The glufosinate-susceptible maize hybrid presented higher ammonia accumulations, lower ETR, and high percentage of injuries (100%), which caused total loss of grain production. Considering the evaluated glufosinate-resistant maize hybrids, Viptera-3 and Agrisure-TL presented the highest ammonia accumulations and percentages of injuries, and lower ETR than the other hybrids. The grain yield of glufosinate-resistant maize hybrids was not reduced due to the application of the 500 g ha-1 of glufosinate. Thus, glufosinate-resistant maize hybrids containing the pat gene are resistant to the application of 500 g ha-1 of glufosinate, and this practice can be recommended for maize crops.
市场上有几种含有膦-丙氨酸乙酰转移酶基因(pat)的玉米杂交品种。然而,这些杂交种对草铵膦除草剂的抗性水平不同。本研究的目的是评价含有pat基因(作为选择标记)的玉米杂种对草铵膦的抗性。田间试验于2016/2017作物季在2个试验点进行,采用2×7因子随机区组设计,4个重复。这些处理包括两种草甘膦浓度(0和500 g ha-1)和7个玉米杂交品种,其中6个含有pat基因作为选择标记(Herculex®、agrisu - tl®、Herculex Yieldgard®、Leptra®、Viptera-3®和Power-Core®),1个不含pat基因(VT PRO®)。在不同地点进行了两次野外试验。分析变量为:氨积累、电子传递速率(ETR)、伤害率、百粒重和籽粒产量。草铵膦敏感玉米杂交种氨积累量高,ETR低,伤害率高(100%),造成粮食生产的全部损失。在所评价的抗草铵膦玉米杂交种中,Viptera-3和agrisu - tl的氨积累量和伤害率最高,ETR较低。施用500 g / ha的草铵膦并没有降低抗草铵膦玉米杂交种的产量。因此,含有pat基因的抗草铵膦玉米杂交种对施用500 g / ha的草铵膦具有抗性,这种做法可以推荐用于玉米作物。
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引用次数: 0
Simple sequence repeat markers revealed genetic divergence and population structure of okra [Abelmoschus esculentus] collections of diverse geographic origin 简单序列重复标记揭示了不同地理来源秋葵(Abelmoschus esculentus)的遗传分化和群体结构
Pub Date : 2020-07-20 DOI: 10.21475/ajcs.20.14.07.p984
W. Mohammed, Beyene A. Amelework, H. Shimelis
The study was carried out to assess the genetic diversity and population structure of okra collections from diverse geographic origin using selected polymorphic simple sequence repeat (SSR) markers. Thirty-two okra accessions collected from three geographic regions were genotyped using 16 selected SSR markers. The SSR markers generated a total of 71 loci of which 67% were polymorphic. Genetic distances among accessions ranged from 2.2 to 7.1, with a mean of 4.65. Gene diversity ranged from 0.10 to 0.78 with a mean of 0.52. Genetic clustering classified the accessions into three major clusters and four sub-clusters. Each cluster and sub-cluster consisted of accessions derived from different sources. Analysis of molecular variance revealed that 67%, 81% and 83% of the total genetic variation detected was found within populations of geographic origin, altitude and collection district, respectively. The observed moderate to high population differentiation could partly be attributed to limited germplasm exchange, agro-ecological differences, and partly by selection pressure. The present study revealed the presence of high genetic diversity and population divergence among okra collections from Ethiopia. The study demonstrated that a collection strategy for conservation of okra genetic resources should focus on sampling of representative genotypes covering wide geographic regions and altitudinal ranges of target agro-ecologies.
利用SSR标记对不同产地秋葵的遗传多样性和群体结构进行了分析。利用16个SSR标记对来自3个地理区域的32份秋葵材料进行基因分型。SSR标记共生成71个位点,其中67%为多态性位点。遗传距离在2.2 ~ 7.1之间,平均为4.65。基因多样性范围为0.10 ~ 0.78,平均值为0.52。遗传聚类将材料分为3大聚类和4个子聚类。每个簇和子簇由来自不同来源的条目组成。分子变异分析表明,总遗传变异的67%、81%和83%分别存在于地理来源群体、海拔高度群体和采集区群体内。种群分化程度中至高的部分原因是有限的种质交换和农业生态差异,部分原因是选择压力。本研究揭示了埃塞俄比亚秋葵种质资源存在较高的遗传多样性和种群差异。研究表明,秋葵遗传资源保护的收集策略应侧重于在目标农业生态的广泛地理区域和高度范围内取样具有代表性的基因型。
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引用次数: 1
Phenotypic response of barley landrace and its wild progenitor Hordeum spontaneum from Jordan to salt stress 约旦大麦地方品种及其野生祖先大麦对盐胁迫的表型响应
Pub Date : 2020-07-20 DOI: 10.21475/ajcs.20.14.07.p2277
A. H. A. Ghani, Khalid Al Abbsi, N. Thaher, Saddam Aref Al- Dalain, G. Rabadi, Farah Al Nasir
Populations of barley landrace [Hordeum vulgare (L.)] and wild barley progenitor [Hordeum spontaneum (L.)] were collected from 13 diverse eco-geographical regions in Jordan. In addition to two check varieties (Rum and Acsad 165) were collected from farmers’ fields. In total, 28 genotypes were evaluated during germination, early seedling and maturity stages for salt tolerance. Barley populations were exposed to tap water of 0.85 dS m-1 and saline solutions (NaCl, CaCl2 and MgSO4) of 4, 8, 12 and 16 dS m-1. Final germination percentage, the lengths of shoot and seminal root, growth and yield related traits were significantly decreased by increasing the level of salinity. Proline content was increased by increasing the level of salinity. A high level of salinity of 16 dS m-1 had a strong impact on reduction of grain yield and other agronomic traits in the examined populations. A considerable variation among populations was detectable. Barley landrace and wild barley that were collected from Tafila region were the most tolerant grown populations in Jordan. Interestingly, the ranking pattern of these two populations at the germination and seedling related-traits was consistent with the ranking pattern at the maturity phase. In conclusion, this study indicates that barley landrace and wild barley populations are potential donors for genes of salt tolerance that can be used to enhance salinity tolerance in barley.
在约旦13个不同的生态地理区域收集了大麦乡土品种Hordeum vulgare (L.)和野生大麦祖先Hordeum spontanum (L.)的种群。此外,从农民的田地里采集了两个检查品种(朗姆酒和Acsad 165)。共有28个基因型在萌发、苗期和成熟期对耐盐性进行了评价。大麦群体分别暴露于0.85 dS - m-1的自来水和4、8、12和16 dS - m-1的盐溶液(NaCl、CaCl2和MgSO4)中。随着盐度的升高,最终发芽率、芽、种根长度、生长和产量相关性状均显著降低。脯氨酸含量随盐度的升高而升高。16 dS m-1的高盐度对籽粒产量和其他农艺性状的降低有强烈影响。在人群中可以发现相当大的差异。在约旦,从塔菲拉地区采集的大麦和野生大麦是最具耐受性的生长群体。有趣的是,这两个群体在萌发和幼苗相关性状上的排名模式与成熟期的排名模式一致。综上所述,大麦地方品种和野生大麦群体是潜在的耐盐基因供体,可用于提高大麦的耐盐性。
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引用次数: 2
Associations between microorganism and maize plant to remedy mercury-contaminated soil 微生物与玉米植物修复汞污染土壤的关系
Pub Date : 2020-07-20 DOI: 10.21475/ajcs.20.14.07.p2731
L. Lavezzo, D. Delarica, A. Fostier, E. Rigobelo, S. Kandasamy, G. Lazarovits, Camila Chioda de Almeida, Danilo Olandino Souza, W. J. Melo
Mercury (Hg) is one of the most toxic metals and is not essential for any organism. In this study, the potential of maize plants in association with bacteria to treat oxisol contaminated with Hg (II) was evaluated. The experiment was conducted in a controlled environment, and pots with 2 kg of oxisol were contaminated with HgCl2 solution at a dose of 36 mg kg-1 of Hg in a 7x4 factorial scheme: control (soil without Hg(II) and microorganisms), T2= (soil with Hg(II) and without microorganisms), and T3= soil with Hg(II) + Enterobacter cloacae, T4= Hg(II) + Bacillus subtilis, T5= Hg(II) + Enterobacter sp., T6= Hg(II) + Staphylococcus epidermidis, and T7= Hg(II) + Bacillus sp. Total Hg quantification was performed by atomic absorption spectrophotometry. At the end of the experiment, the soil pH was significantly lower (0.3 to 0.4 pH unit) in the T2 (no inoculation), Enterobacter cloacae, Enterobacter sp. and Bacillus sp. treatments. Neither contamination of soil with Hg nor plant associations with bacteria led to differences in the root dry mass of maize plants. Maize plants associated with Staphylococcus epidermidis and Bacillus sp. bacteria had lower shoot biomass (71 and 50%) compared to the treatment 2. The best remedial effect was observed with the association of maize plants with Bacillus sp., which recovered 19.67% of Hg(II) in the soil when compared to control and treatment 2 and treatment with B. subtilis. The recommendation is the use of B. subtilis to decrease the toxicity caused by Hg(II).
汞(Hg)是毒性最强的金属之一,对任何生物体都不是必需的。在本研究中,评价了玉米植株与细菌联合处理被汞(II)污染的草甸的潜力。实验在受控环境下进行,在7x4析因方案中,以36mg kg-1的汞浓度污染装有2kg氧化索的容器。对照(不含Hg(II)和微生物的土壤),T2=含Hg(II)和不含微生物的土壤),T3=含Hg(II) +阴沟肠杆菌的土壤,T4= Hg(II) +枯草芽孢杆菌,T5= Hg(II) +肠杆菌,T6= Hg(II) +表皮葡萄球菌,T7= Hg(II) +芽孢杆菌。采用原子吸收分光光度法测定总汞。试验结束时,T2(未接种)、阴沟肠杆菌、肠杆菌和芽孢杆菌处理的土壤pH值显著降低(0.3 ~ 0.4 pH单位)。土壤汞污染和植物与细菌的关联都没有导致玉米植株根系干质量的差异。与处理2相比,与表皮葡萄球菌和芽孢杆菌相关的玉米植株的茎部生物量(71%和50%)较低。与对照、处理2和枯草芽孢杆菌处理相比,玉米植株与芽孢杆菌联合处理修复土壤Hg(II)的效果最好,回收率为19.67%。建议使用枯草芽孢杆菌来降低汞(II)引起的毒性。
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引用次数: 0
Selection of Saccharum spp. rhizobacteria with growth-promoting properties using PCA analysis 利用主成分分析法筛选具有促生长特性的糖根菌
Pub Date : 2020-07-01 DOI: 10.21475/ajcs.20.14.07.p2698
R. M. Santos, E. Rigobelo
The search for plant growth-promoting rhizobacteria is an ongoing need for the development of new bioinoculants for use in various crops, including sugarcane. Bacterial strains with various plant growth-promoting properties can contribute to sustainable agricultural production. The present study aimed to isolate, characterize and select sugarcane rhizobacteria from six different varieties through principal components analysis. This study selected 167 bacterial strains with the ability to fix nitrogen, produce indolacetic acid, exhibit cellulolytic activity, and solubilize phosphate and potassium were isolated. Of these 167 bacterial strains, seven were selected by principal component analysis and identified as belonging to the genera Staphylococcus, Enterobacter, Bacillus and Achromobacter. Bacillus thuringiensis IP21 presented higher potential for nitrogen fixation and CaPO4 and AlPO4 solubilization and a lower potential for K solubilization in sugarcane. Enterobacter asburiae IP24 was efficient in indolacetic acid production and CaPO4 and FePO4 solubilization and inefficient for Araxá apatite solubilization.
寻找促进植物生长的根瘤菌是开发用于包括甘蔗在内的各种作物的新型生物接种剂的持续需求。具有各种植物生长促进特性的菌株可以为可持续农业生产做出贡献。本研究旨在通过主成分分析对6个不同品种的甘蔗根瘤菌进行分离、鉴定和筛选。本研究选取了167株具有固定氮、产生吲哚乙酸、水解纤维素、溶解磷酸盐和钾的细菌进行分离。167株细菌经主成分分析筛选出7株,分别归属于葡萄球菌属、肠杆菌属、芽孢杆菌属和无色杆菌属。苏云金芽孢杆菌IP21在甘蔗中具有较高的固氮和增溶CaPO4和AlPO4的潜力,而增溶K的潜力较低。asbureenterobacter IP24对吲哚乙酸的生成和对CaPO4和FePO4的增溶效果较好,对arax磷灰石的增溶效果较差。
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引用次数: 0
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