Natural plasmids are present in fermented foods ranging from yogurts to sourdoughs, sausages, kimchi or pickling crabs. In this study, we describe the natural plasmids present in a product obtained by fermentation of bovine milk and colostrum. We used a microarray consisting in a chip covered in short DNA sequences that are specific to target microorganisms for a total of approximately 12,000 species. As far as plasmids are concerned, the array yielded 48 iterations, each one corresponding to a unique plasmid target. We discuss the putative role of some of these plasmids in contributing to the known properties of probiotics in supporting health and longevity.
{"title":"Natural Plasmids in a Swiss Fermented Milk and Colostrum Product assessed by Microbiome Array","authors":"S. Pacini, M. Ruggiero","doi":"10.18689/mjim-1000123","DOIUrl":"https://doi.org/10.18689/mjim-1000123","url":null,"abstract":"Natural plasmids are present in fermented foods ranging from yogurts to sourdoughs, sausages, kimchi or pickling crabs. In this study, we describe the natural plasmids present in a product obtained by fermentation of bovine milk and colostrum. We used a microarray consisting in a chip covered in short DNA sequences that are specific to target microorganisms for a total of approximately 12,000 species. As far as plasmids are concerned, the array yielded 48 iterations, each one corresponding to a unique plasmid target. We discuss the putative role of some of these plasmids in contributing to the known properties of probiotics in supporting health and longevity.","PeriodicalId":304005,"journal":{"name":"Madridge Journal of Immunology","volume":"32 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120994423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sexually transmitted diseases are an important issue in the world because to the high incidence and the potential permanent consequences of general and reproductive health. Sexually transmitted diseases are contagious diseases that are transmitted by direct contact during sexual intercourse (vaginal, oral or anal) with an infected person.
{"title":"Adolescent Venereal Diseases","authors":"Siniša Franjić","doi":"10.18689/mjim-1000122","DOIUrl":"https://doi.org/10.18689/mjim-1000122","url":null,"abstract":"Sexually transmitted diseases are an important issue in the world because to the high incidence and the potential permanent consequences of general and reproductive health. Sexually transmitted diseases are contagious diseases that are transmitted by direct contact during sexual intercourse (vaginal, oral or anal) with an infected person.","PeriodicalId":304005,"journal":{"name":"Madridge Journal of Immunology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129004934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
It is one of the most common endocrine system disorders characterized by irregular menses, hyper androgenism, and polycystic ovaries that affect women in their reproductive age. Family history of PCOD may have getting the chance about 50%, followed by insulin resistance and inflammation has all been linked to excess release of androgen production. But now a day’s most of the girls and women addict to eat junk foods like fried food items and that food doesn’t have a good quality. PCOD may induces the other health related problems like anxiety, depression, irregular menses, insulin resistance, sleep apnea, and high blood pressure.
{"title":"In Current Scenario various Etiological and Risk Factors those induce Polycystic Ovarian Syndrome: A Brief Review","authors":"G. Nagaraju, S. Subrahmanyam","doi":"10.18689/mjim-1000121","DOIUrl":"https://doi.org/10.18689/mjim-1000121","url":null,"abstract":"It is one of the most common endocrine system disorders characterized by irregular menses, hyper androgenism, and polycystic ovaries that affect women in their reproductive age. Family history of PCOD may have getting the chance about 50%, followed by insulin resistance and inflammation has all been linked to excess release of androgen production. But now a day’s most of the girls and women addict to eat junk foods like fried food items and that food doesn’t have a good quality. PCOD may induces the other health related problems like anxiety, depression, irregular menses, insulin resistance, sleep apnea, and high blood pressure.","PeriodicalId":304005,"journal":{"name":"Madridge Journal of Immunology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122381217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To evaluate the anticancer activity of fruits of Momordica dioica by using MTT assay. The aqueous extract of fruits of Momordica dioica was tested against ovarian carcinoma of PA-I cell lines and human cervical cancer of Hela cell line at various concentrations. The anticancer effect of the aqueous extracts on cell inhibition was studied using MTT assay. The results showed that aqueous extract of fruits of Momordica dioica inhibited growth of PA-I and Hela cells at IC 50 concentration is 40 µg/mL respectively. Alterations in the cell morphology were also observed after treatment of the cell lines with fruit extract. The present work revealed that Momordica dioica contains some important chemical constituents extracted using aqueous as solvent that can be used further in the management of cancer treatment.
{"title":"Anticancer activity of Fruits of Momordica Dioica by using MTT assay","authors":"R. Ahirrao","doi":"10.18689/mjim-1000120","DOIUrl":"https://doi.org/10.18689/mjim-1000120","url":null,"abstract":"To evaluate the anticancer activity of fruits of Momordica dioica by using MTT assay. The aqueous extract of fruits of Momordica dioica was tested against ovarian carcinoma of PA-I cell lines and human cervical cancer of Hela cell line at various concentrations. The anticancer effect of the aqueous extracts on cell inhibition was studied using MTT assay. The results showed that aqueous extract of fruits of Momordica dioica inhibited growth of PA-I and Hela cells at IC 50 concentration is 40 µg/mL respectively. Alterations in the cell morphology were also observed after treatment of the cell lines with fruit extract. The present work revealed that Momordica dioica contains some important chemical constituents extracted using aqueous as solvent that can be used further in the management of cancer treatment.","PeriodicalId":304005,"journal":{"name":"Madridge Journal of Immunology","volume":"116 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122987643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gliomas are the most common subgroup of primary malignant brain tumors in adults, constituting >40% of all primary CNS neoplasms. Although all gliomas originate from neuroepithelial tissues, they vary considerably in morphology, location, genetic alterations and in their response to therapy. The most malignant of gliomas (Grade IV) is Glioblastoma Multiforme (GBM), causing over 10,000 deaths each year in the US alone. Despite robust therapeutic advances, median survival for GBM still remains 14-20 months with very high tumor recurrence rate. Various investigation modalities are available for establishing diagnosis of glioma, like: CT scan, MRI, X-ray, spinal tap, angiogram, myelogram & biopsy, though, histopathology represents the gold standard for their typing & grading. However, even this remains unsatisfactory because of the lack of reproducibility and absence of precision. Development of objective, diagnostic, prognostic & predictive markers for these lethal neoplasms is therefore a priority. Biomarkers for glioma can be identified in various biological samples like: DNA, mRNA, cell surface receptors, transcription factors, secretory proteins, metabolites or processes such as proliferation, angiogenesis or apoptosis. Tumor biomarkers help oncologists in managing gliomas at various levels, from screening till assessment of longitudinal response to therapy. Identifying the molecular & pathogenetic characteristics of glioma regulation network may increase the precision of customized medication. Further, the proteomic approach has the potential to identify novel diagnostic, prognostic and therapeutic biomarkers. In the near future, improved proteomic profiling is anticipated to bring about a merger of biology, engineering and informatics, with a profound impact on glioma research and treatment. Optimization of experimental design and validation in independent cohorts, improved multiplex proteomic methodologies and bioinformatics tools, and their integration with genetic and metabolomic profiling technologies promise to play critical roles in the post proteomics era of cancer diagnosis and treatment.
{"title":"Review on Immunological Biomarkers in Gliomas","authors":"C. Nagalakshmi, N. Santhosh","doi":"10.18689/mjim-1000119","DOIUrl":"https://doi.org/10.18689/mjim-1000119","url":null,"abstract":"Gliomas are the most common subgroup of primary malignant brain tumors in adults, constituting >40% of all primary CNS neoplasms. Although all gliomas originate from neuroepithelial tissues, they vary considerably in morphology, location, genetic alterations and in their response to therapy. The most malignant of gliomas (Grade IV) is Glioblastoma Multiforme (GBM), causing over 10,000 deaths each year in the US alone. Despite robust therapeutic advances, median survival for GBM still remains 14-20 months with very high tumor recurrence rate. Various investigation modalities are available for establishing diagnosis of glioma, like: CT scan, MRI, X-ray, spinal tap, angiogram, myelogram & biopsy, though, histopathology represents the gold standard for their typing & grading. However, even this remains unsatisfactory because of the lack of reproducibility and absence of precision. Development of objective, diagnostic, prognostic & predictive markers for these lethal neoplasms is therefore a priority. Biomarkers for glioma can be identified in various biological samples like: DNA, mRNA, cell surface receptors, transcription factors, secretory proteins, metabolites or processes such as proliferation, angiogenesis or apoptosis. Tumor biomarkers help oncologists in managing gliomas at various levels, from screening till assessment of longitudinal response to therapy. Identifying the molecular & pathogenetic characteristics of glioma regulation network may increase the precision of customized medication. Further, the proteomic approach has the potential to identify novel diagnostic, prognostic and therapeutic biomarkers. In the near future, improved proteomic profiling is anticipated to bring about a merger of biology, engineering and informatics, with a profound impact on glioma research and treatment. Optimization of experimental design and validation in independent cohorts, improved multiplex proteomic methodologies and bioinformatics tools, and their integration with genetic and metabolomic profiling technologies promise to play critical roles in the post proteomics era of cancer diagnosis and treatment.","PeriodicalId":304005,"journal":{"name":"Madridge Journal of Immunology","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122820766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Sanyaolu, D. Hammoudi, O. Badaru, Ifeoluwa Adekanye, Adeola Akinwekomi, Kateryna Shyshkova
The latency of resting HIV-1 in CD4+ T cells is the obstacle to complete destruction of the virus in patients that have been given highly active antiretroviral therapy (HAART). This latency occurs early during acute infection but remains silent in the host cells; however it is still capable of making infectious proviruses if antiviral therapy is stopped. The goal of HAART therapy is to reduce the replication levels of HIV-1 to undetectable levels in serum of infected individuals. HIV-1 therapy involves the use of multiple drugs because of the ability of the virus to easily acquire drug resistance to an inhibitor. Resistance develops due to the diversity of HIV-1 genome within several individuals. Gene therapy approaches have been shown to be somewhat safer but have not been proven yet in human models. The process of HIV-1 latency is multifactorial and involves several molecular pathways which are still being studied. This study reviews HIV-1 latency as well as its therapeutic implication.
{"title":"The Mechanism of HIV-1 Latency and Therapeutic Implication","authors":"A. Sanyaolu, D. Hammoudi, O. Badaru, Ifeoluwa Adekanye, Adeola Akinwekomi, Kateryna Shyshkova","doi":"10.18689/mjim-1000118","DOIUrl":"https://doi.org/10.18689/mjim-1000118","url":null,"abstract":"The latency of resting HIV-1 in CD4+ T cells is the obstacle to complete destruction of the virus in patients that have been given highly active antiretroviral therapy (HAART). This latency occurs early during acute infection but remains silent in the host cells; however it is still capable of making infectious proviruses if antiviral therapy is stopped. The goal of HAART therapy is to reduce the replication levels of HIV-1 to undetectable levels in serum of infected individuals. HIV-1 therapy involves the use of multiple drugs because of the ability of the virus to easily acquire drug resistance to an inhibitor. Resistance develops due to the diversity of HIV-1 genome within several individuals. Gene therapy approaches have been shown to be somewhat safer but have not been proven yet in human models. The process of HIV-1 latency is multifactorial and involves several molecular pathways which are still being studied. This study reviews HIV-1 latency as well as its therapeutic implication.","PeriodicalId":304005,"journal":{"name":"Madridge Journal of Immunology","volume":"310 5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127493539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Gadiri, H. Meriche, A. Alliouch-Kerboua, A. Atik
Results: The mean age of patients was 51 years, the diagnosis was: 14 cases of GPA, 21 cases of microscopic polyangiitis (MPA), 04 cases of EGPA, 53 subjects had signs of overlap between the GPA and MPA. The clinical picture was dominated by renal disease followed by lung disease and rheumatologic signs. Some patients had cardiac involvement. 71 patients had p-ANCA (77, 2%), of which 43 was anti-MPO specificity (46.7%), 21 patients had c-ANCA (22.8%), including 9 with a specific anti-PR3 (98%), 40 patients showed no 2 searched specificities (43.4%).
{"title":"ANCA Vasculitis in Algeria","authors":"S. Gadiri, H. Meriche, A. Alliouch-Kerboua, A. Atik","doi":"10.18689/MJIM-1000117","DOIUrl":"https://doi.org/10.18689/MJIM-1000117","url":null,"abstract":"Results: The mean age of patients was 51 years, the diagnosis was: 14 cases of GPA, 21 cases of microscopic polyangiitis (MPA), 04 cases of EGPA, 53 subjects had signs of overlap between the GPA and MPA. The clinical picture was dominated by renal disease followed by lung disease and rheumatologic signs. Some patients had cardiac involvement. 71 patients had p-ANCA (77, 2%), of which 43 was anti-MPO specificity (46.7%), 21 patients had c-ANCA (22.8%), including 9 with a specific anti-PR3 (98%), 40 patients showed no 2 searched specificities (43.4%).","PeriodicalId":304005,"journal":{"name":"Madridge Journal of Immunology","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130869305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this editorial we propose an approach for improving brain lymphatic flow in autism through lymphatic drainage massage that is a strategy successfully utilized in a variety of neurological conditions where lymph coming from the brain finds obstacles at the level of deep cervical nodes. We estimate that manual lymphatic massage at the level of deep cervical nodes improves brain lymphatic drainage possibly resulting in decrease of neuroinflammation that is a hallmark of autism. In addition to stimulating lymphatic drainage, we theorize that such an approach may work also as vagus nerve stimulation with the additional benefit of targeting the larynx in a manner similar to that outlined for laryngeal manual therapy. This therapy is utilized for the treatment of dysphonia, and its effects on speech, a function that is typically altered in autism, may contribute to the overall improvement of symptoms. We propose that this type of inexpensive, safe and accessible approach can be beneficial to autistic subjects and constitute a new and promising treatment. We are convinced that the described approach will play a pivotal role in future options for autism treatment, possibly in combination with other therapeutic approaches such as behavioral therapies or nutritional supplementation.
{"title":"Manual Lymphatic Drainage in Autism Treatment","authors":"Nicola Antonucci, S. Pacini, M. Ruggiero","doi":"10.18689/MJIM-1000116","DOIUrl":"https://doi.org/10.18689/MJIM-1000116","url":null,"abstract":"In this editorial we propose an approach for improving brain lymphatic flow in autism through lymphatic drainage massage that is a strategy successfully utilized in a variety of neurological conditions where lymph coming from the brain finds obstacles at the level of deep cervical nodes. We estimate that manual lymphatic massage at the level of deep cervical nodes improves brain lymphatic drainage possibly resulting in decrease of neuroinflammation that is a hallmark of autism. In addition to stimulating lymphatic drainage, we theorize that such an approach may work also as vagus nerve stimulation with the additional benefit of targeting the larynx in a manner similar to that outlined for laryngeal manual therapy. This therapy is utilized for the treatment of dysphonia, and its effects on speech, a function that is typically altered in autism, may contribute to the overall improvement of symptoms. We propose that this type of inexpensive, safe and accessible approach can be beneficial to autistic subjects and constitute a new and promising treatment. We are convinced that the described approach will play a pivotal role in future options for autism treatment, possibly in combination with other therapeutic approaches such as behavioral therapies or nutritional supplementation.","PeriodicalId":304005,"journal":{"name":"Madridge Journal of Immunology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125442017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aims to examine hematological parameters for early diagnosis of retained placenta (RP) during three stages of postpartum periods in dairy cattle. For this purpose, thirty post calving dairy cows with RP (group I) and healthy (group II) were selected (n=15 each). The cows were placed into 3 stages of period, from 1 to 3 weeks after parturition. Then, blood samples were collected from two groups. No significant differences were observed in hematological indices between two groups of cattle. The results showed that RP does not cause to significant changes in some hematological parameters as well as total protein and albumin (Alb) when compared to the healthy cows (p<0.05). Also, significant increases in the concentration of fibrinogen (Fb) was observed in diseased cows during 1 to 2 weeks when compared to the healthy (p<0.05). Our results indicated, in spite of hematological parameter, some serum indices including Fib could be successfully applied as indicators for RP in post calving dairy cattle. Therefore, blood indices cannot be used for early diagnosis of RP in dairy cattle.
{"title":"Towards to Changes in Blood Indices as Early Diagnosis of Retained Placenta","authors":"E. Fathi, H. Hamali","doi":"10.18689/MJIM-1000115","DOIUrl":"https://doi.org/10.18689/MJIM-1000115","url":null,"abstract":"This study aims to examine hematological parameters for early diagnosis of retained placenta (RP) during three stages of postpartum periods in dairy cattle. For this purpose, thirty post calving dairy cows with RP (group I) and healthy (group II) were selected (n=15 each). The cows were placed into 3 stages of period, from 1 to 3 weeks after parturition. Then, blood samples were collected from two groups. No significant differences were observed in hematological indices between two groups of cattle. The results showed that RP does not cause to significant changes in some hematological parameters as well as total protein and albumin (Alb) when compared to the healthy cows (p<0.05). Also, significant increases in the concentration of fibrinogen (Fb) was observed in diseased cows during 1 to 2 weeks when compared to the healthy (p<0.05). Our results indicated, in spite of hematological parameter, some serum indices including Fib could be successfully applied as indicators for RP in post calving dairy cattle. Therefore, blood indices cannot be used for early diagnosis of RP in dairy cattle.","PeriodicalId":304005,"journal":{"name":"Madridge Journal of Immunology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123388404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jm Favela-Hernández, I. Balderas-Rentería, G. Guerrero
1 Universidad Autónoma de Zacatecas, Unidad Académica de Ciencias Biológicas, Lab de Immunobiologia, Campus II-UAZ, Zacatecas, Zac, México 2Instituto Multidisciplinario de Ciencias (AVICENA), Campus Torreón, Coahuila, México 3 Universidad Autónoma de Nuevo León, Departamento de Investigación y Posgrado de la, Facultad de Ciencias Químicas, Lab de Ingeniería Genética y Genómica, Monterrey, Nvo León, México
{"title":"The Potential of a Commercial Product based on Bacillus thuringiensis Cry1A-Cry2A Toxins as an Immunogen and Adjuvant","authors":"Jm Favela-Hernández, I. Balderas-Rentería, G. Guerrero","doi":"10.18689/mjim-1000114","DOIUrl":"https://doi.org/10.18689/mjim-1000114","url":null,"abstract":"1 Universidad Autónoma de Zacatecas, Unidad Académica de Ciencias Biológicas, Lab de Immunobiologia, Campus II-UAZ, Zacatecas, Zac, México 2Instituto Multidisciplinario de Ciencias (AVICENA), Campus Torreón, Coahuila, México 3 Universidad Autónoma de Nuevo León, Departamento de Investigación y Posgrado de la, Facultad de Ciencias Químicas, Lab de Ingeniería Genética y Genómica, Monterrey, Nvo León, México","PeriodicalId":304005,"journal":{"name":"Madridge Journal of Immunology","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133225528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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