Ethanol extract of arabica coffee leaves has various nutritious compounds such as flavonoids. With the existence of these beneficial compounds, extract can be developed into a facial wash gel so that it can be applied to facial skin care. In making facial wash gel, a gelling agent is required as a base to form a gel consistency. This study aims to determine the consistency of a good gel base in facial wash using the gelling agents Carbopol (F1 and F2), HPMC (F3 and F4) and Na CMC (F5 and F6). Results of the organoleptic test gel facial wash were blackish brown in color with a distinctive fragrance and semi-solid form on all formulation. The test results on the F1, F2, F3, F4, F5 and F6 obtained successive results of pH 4.68; 4.61; 4.76; 4.79; 4.67; and 4.67; viscosity 2468 cp; 3414 cp; 3420 cp; 3543 cp; 3379 cps and 3461 cps; foam stability 95.00%; 96.92%; 98.14%; 97.91%; 95.74% and 94.44%. Homogeneity and stability results show homogeneous and stable results in all formulas. Based on the research results, it can be concluded that all the formulas made meet the SNI requirements.
{"title":"FORMULASI SEDIAAN GEL FACIAL WASH EKSTRAK ETANOL DAUN KOPI ARABIKA (Coffea arabica L.) DENGAN BERBAGAI GELLING AGENT","authors":"Yahdian Rasyadi, Diza Sartika, Nadya Dini Fitri","doi":"10.36387/jifi.v6i1.1373","DOIUrl":"https://doi.org/10.36387/jifi.v6i1.1373","url":null,"abstract":"Ethanol extract of arabica coffee leaves has various nutritious compounds such as flavonoids. With the existence of these beneficial compounds, extract can be developed into a facial wash gel so that it can be applied to facial skin care. In making facial wash gel, a gelling agent is required as a base to form a gel consistency. This study aims to determine the consistency of a good gel base in facial wash using the gelling agents Carbopol (F1 and F2), HPMC (F3 and F4) and Na CMC (F5 and F6). Results of the organoleptic test gel facial wash were blackish brown in color with a distinctive fragrance and semi-solid form on all formulation. The test results on the F1, F2, F3, F4, F5 and F6 obtained successive results of pH 4.68; 4.61; 4.76; 4.79; 4.67; and 4.67; viscosity 2468 cp; 3414 cp; 3420 cp; 3543 cp; 3379 cps and 3461 cps; foam stability 95.00%; 96.92%; 98.14%; 97.91%; 95.74% and 94.44%. Homogeneity and stability results show homogeneous and stable results in all formulas. Based on the research results, it can be concluded that all the formulas made meet the SNI requirements.","PeriodicalId":306551,"journal":{"name":"Jurnal Insan Farmasi Indonesia","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130354354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fitrah Shafran Ilahi, Abdul Mahmud Yumassik, Mochammad Maulidie Alfiannor Saputera
Digital pharmacy has been growing in the current era, so the need for an application to help the performance of a pharmacist is indispensable. Pharmacy management information system applications that have been circulating are known to have not used a drug expiration date notification system to email. The purpose of this study was to find out whether the drug storage application design can control drug expiration in a website-based pharmacy. The research method is carried out by making website-based software by applying data flow diagrams (DFD). The research procedure is to first design a data flow diagram consisting of drug data input, purchase transaction data input, sales transaction data input, drug storage data input and transaction report input. After that, create a use case design consisting of two, namely admin and cashier. The last step is to create a block diagram flow with notifications of drugs approaching expiration in the form of incoming e-mails to users. The results showed that the design of the drug storage application system that was made could operate properly and had been tested independently. The drug storage application has not yet been tested by the public, so further testing is needed.
{"title":"PEMBUATAN APLIKASI PENYIMPANAN OBAT (APO) DALAM PENGONTROLAN MASA KADALUARSA OBAT DI APOTEK BERBASIS WEB","authors":"Fitrah Shafran Ilahi, Abdul Mahmud Yumassik, Mochammad Maulidie Alfiannor Saputera","doi":"10.36387/jifi.v6i1.1301","DOIUrl":"https://doi.org/10.36387/jifi.v6i1.1301","url":null,"abstract":"Digital pharmacy has been growing in the current era, so the need for an application to help the performance of a pharmacist is indispensable. Pharmacy management information system applications that have been circulating are known to have not used a drug expiration date notification system to email. The purpose of this study was to find out whether the drug storage application design can control drug expiration in a website-based pharmacy. The research method is carried out by making website-based software by applying data flow diagrams (DFD). The research procedure is to first design a data flow diagram consisting of drug data input, purchase transaction data input, sales transaction data input, drug storage data input and transaction report input. After that, create a use case design consisting of two, namely admin and cashier. The last step is to create a block diagram flow with notifications of drugs approaching expiration in the form of incoming e-mails to users. The results showed that the design of the drug storage application system that was made could operate properly and had been tested independently. The drug storage application has not yet been tested by the public, so further testing is needed.","PeriodicalId":306551,"journal":{"name":"Jurnal Insan Farmasi Indonesia","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133815964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mindiya Fatmi, Septia Andini, R. Ambarwati, Ikrom Khaulah
Hair dye is a cosmetic preparation intended to give the hair a new color or restore the original color. Purple sweet potato tuber skin contains more anthocyanin that can be used in the manufacture of hair dye preparations. The extract was prepared by maceration using 96% ethanol. Hair dyes were made into 4 formulas: Formula 0 was not added with a concentration of copper II sulfate, while Formulas 1, 2, and 3 were added with copper II sulfate concentrations of 0.5%, 1%, and 1.5%. The tests included organoleptic tests, homogeneity, pH, viscosity, hair coloring stability tests, including stability tests against washing and sunburn, and preference tests. The results showed that the hair dye gel preparations were homogeneous, had a distinctive odor of hair dye, and had brown (F0), brownish green (F1), and blackish green (F2 and F3) colors. The pH of the preparation ranged from 4.6 to 6.3, and the viscosity test ranged from 8243.5-8583.5 Cp. In the staining stabili
{"title":"EFEK PEWARNAAN RAMBUT DARI EKSTRAK KULIT UMBI UBI UNGU (Ipomoea batatas L.) DENGAN VARIASI KONSENTRASI CU SULFAT SEBAGAI PEMBANGKIT WARNA","authors":"Mindiya Fatmi, Septia Andini, R. Ambarwati, Ikrom Khaulah","doi":"10.36387/jifi.v6i1.1368","DOIUrl":"https://doi.org/10.36387/jifi.v6i1.1368","url":null,"abstract":"Hair dye is a cosmetic preparation intended to give the hair a new color or restore the original color. Purple sweet potato tuber skin contains more anthocyanin that can be used in the manufacture of hair dye preparations. The extract was prepared by maceration using 96% ethanol. Hair dyes were made into 4 formulas: Formula 0 was not added with a concentration of copper II sulfate, while Formulas 1, 2, and 3 were added with copper II sulfate concentrations of 0.5%, 1%, and 1.5%. The tests included organoleptic tests, homogeneity, pH, viscosity, hair coloring stability tests, including stability tests against washing and sunburn, and preference tests. The results showed that the hair dye gel preparations were homogeneous, had a distinctive odor of hair dye, and had brown (F0), brownish green (F1), and blackish green (F2 and F3) colors. The pH of the preparation ranged from 4.6 to 6.3, and the viscosity test ranged from 8243.5-8583.5 Cp. In the staining stabili","PeriodicalId":306551,"journal":{"name":"Jurnal Insan Farmasi Indonesia","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126671811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Novia Ariani, Erna Prihandiwati, Hairulia Intan Wuri, M. Apriawan
South Kalimantan Province has the highest prevalence rate in Indonesia, which is 44.13%. Data from the Banjarmasin Indah Health Center Report for 2021 shows that there are 1,161 cases of hypertension and have an elderly Posyandu with the highest disease of hypertension. Efforts to improve the health status of the elderly by increasing the knowledge of the elderly through the provision of drug information, one of which is through the media of leaflets. This study aims to determine the effectiveness of providing information on hypertension drugs using leaflets for elderly patients at the Posyandu Lansia Melati. The study used a quasi-experimental design with the one group pre and post test method. Data collection prospectively in March-April 2022 using a total sampling technique. Data analysis using the Wilcoxon test to see the effectiveness of providing drug information. The results showed the level of knowledge before being given drug information was in the good category at 23.22% and after being given drug information through leaflets there was a significant change with the good category at 100% (p <0.05). The conclusion of the study showed providing information on hypertension drugs using leaflets was effective in increasing the level of knowledge of hypertensive patients.
{"title":"PEMANFAATAN MEDIA LEAFLET TERHADAP TINGKAT PENGETAHUAN PASIEN HIPERTENSI LANSIA DI POSYANDU LANSIA MELATI","authors":"Novia Ariani, Erna Prihandiwati, Hairulia Intan Wuri, M. Apriawan","doi":"10.36387/jifi.v6i1.1379","DOIUrl":"https://doi.org/10.36387/jifi.v6i1.1379","url":null,"abstract":"South Kalimantan Province has the highest prevalence rate in Indonesia, which is 44.13%. Data from the Banjarmasin Indah Health Center Report for 2021 shows that there are 1,161 cases of hypertension and have an elderly Posyandu with the highest disease of hypertension. Efforts to improve the health status of the elderly by increasing the knowledge of the elderly through the provision of drug information, one of which is through the media of leaflets. This study aims to determine the effectiveness of providing information on hypertension drugs using leaflets for elderly patients at the Posyandu Lansia Melati. The study used a quasi-experimental design with the one group pre and post test method. Data collection prospectively in March-April 2022 using a total sampling technique. Data analysis using the Wilcoxon test to see the effectiveness of providing drug information. The results showed the level of knowledge before being given drug information was in the good category at 23.22% and after being given drug information through leaflets there was a significant change with the good category at 100% (p <0.05). The conclusion of the study showed providing information on hypertension drugs using leaflets was effective in increasing the level of knowledge of hypertensive patients.","PeriodicalId":306551,"journal":{"name":"Jurnal Insan Farmasi Indonesia","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131418227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mahfur, Khafidz Mahbub, Nada Safira Salsabila, M. Istiqomah
Sponges are known have many metabolites compounds and potential to be used as raw materials for medicines. spongesare have the ability to form symbiosis with microorganisms. Rhabdastrela sp is have to contain various secondary metabolites, especially the Triterpenes group. The fermentation process is a method to produce abundant secondary metabolite from microorganisms such as symbiont fungi. This study aims to determine the optimal fermentation time for the symbiont fungus Rhabdastrella sp. by looking at the secondary metabolites produced and their antibacterial activity. This research is included of experimental research. Cultivation of the sponge Rhabdastrella sp. for the growth of symbiont fungi. Followed by a fermentation process with variations in time with 2,4,6,8,10,12, and 14 days to get the secondary metabolites produced. Liquid liquid extraction was carried out to obtain secondary metabolites. The final stage is TLC profile and test the antibacterial activity of extract using the well-diffusion method. The results showed that the long fermentation time affected the secondary metabolites obtained, and their antibacterial activity. The profile of secondary metabolites showed that the 12th day of fermentation had the most complex secondary metabolites and the highest yield and had a clear zone of 12.5 ± 0.26 mm.
{"title":"OPTIMASI WAKTU FERMENTASI JAMUR SIMBION DARI SPONGE Rhabdastrella SP. DAN UJI AKTIVITAS ANTIBAKTERINYA","authors":"M. Mahfur, Khafidz Mahbub, Nada Safira Salsabila, M. Istiqomah","doi":"10.36387/jifi.v6i1.1333","DOIUrl":"https://doi.org/10.36387/jifi.v6i1.1333","url":null,"abstract":"Sponges are known have many metabolites compounds and potential to be used as raw materials for medicines. spongesare have the ability to form symbiosis with microorganisms. Rhabdastrela sp is have to contain various secondary metabolites, especially the Triterpenes group. The fermentation process is a method to produce abundant secondary metabolite from microorganisms such as symbiont fungi. This study aims to determine the optimal fermentation time for the symbiont fungus Rhabdastrella sp. by looking at the secondary metabolites produced and their antibacterial activity. This research is included of experimental research. Cultivation of the sponge Rhabdastrella sp. for the growth of symbiont fungi. Followed by a fermentation process with variations in time with 2,4,6,8,10,12, and 14 days to get the secondary metabolites produced. Liquid liquid extraction was carried out to obtain secondary metabolites. The final stage is TLC profile and test the antibacterial activity of extract using the well-diffusion method. The results showed that the long fermentation time affected the secondary metabolites obtained, and their antibacterial activity. The profile of secondary metabolites showed that the 12th day of fermentation had the most complex secondary metabolites and the highest yield and had a clear zone of 12.5 ± 0.26 mm.","PeriodicalId":306551,"journal":{"name":"Jurnal Insan Farmasi Indonesia","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125510223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sasqia Indri Maharani, Hery Muhammad Ansory, Inaratul Rhizky Hanifah
Isomyristicin results from myristicin isomerization and is reported to have the same biological activity as myristicin, an antidepressant. Myristicin at a specific dose causes toxicity, so it is necessary to study isomyristicin, which is estimated to have the same biological activity as myristicin. However, there has been no test related to isomyristicin having antidepressant activity. Therefore, this study aimed to determine the antidepressant activity and the optimum dose of isomyristicin to decrease the immobility time of male white mice (Mus musculus). This study used 30 male white mice divided into six groups: positive control, negative control, normal control, and isomyristicin dose 1.5 mg/Kg BW, 3 mg/Kg BW, and 6 mg/Kg BW mice. The method to measure the immobility time of male white mice is the Forced Swimming Test (FST) and Tail Suspension Test (TST) to induce depression. To analyze the normality data using a Shapiro Wilk and a one-way ANOVA to analyze the significant difference in the data. The result showed all doses of isomyristicin have the ability to antidepressant, and the optimal dose was 1,5 mg/kg BW mice with 82 % as the decrease percentage and proved by post hoc with sig. p (<0,05).
{"title":"UJI AKTIVITAS ANTIDEPRESAN ISOMIRISTISIN TERHADAP IMMOBILITY TIME MENCIT PUTIH JANTAN (MUS MUSCULUS)","authors":"Sasqia Indri Maharani, Hery Muhammad Ansory, Inaratul Rhizky Hanifah","doi":"10.36387/jifi.v6i1.1279","DOIUrl":"https://doi.org/10.36387/jifi.v6i1.1279","url":null,"abstract":"Isomyristicin results from myristicin isomerization and is reported to have the same biological activity as myristicin, an antidepressant. Myristicin at a specific dose causes toxicity, so it is necessary to study isomyristicin, which is estimated to have the same biological activity as myristicin. However, there has been no test related to isomyristicin having antidepressant activity. Therefore, this study aimed to determine the antidepressant activity and the optimum dose of isomyristicin to decrease the immobility time of male white mice (Mus musculus). This study used 30 male white mice divided into six groups: positive control, negative control, normal control, and isomyristicin dose 1.5 mg/Kg BW, 3 mg/Kg BW, and 6 mg/Kg BW mice. The method to measure the immobility time of male white mice is the Forced Swimming Test (FST) and Tail Suspension Test (TST) to induce depression. To analyze the normality data using a Shapiro Wilk and a one-way ANOVA to analyze the significant difference in the data. The result showed all doses of isomyristicin have the ability to antidepressant, and the optimal dose was 1,5 mg/kg BW mice with 82 % as the decrease percentage and proved by post hoc with sig. p (<0,05).","PeriodicalId":306551,"journal":{"name":"Jurnal Insan Farmasi Indonesia","volume":"114 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116150006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amaliyah Wahyuni, Abdul Mahmud Yumassik, Isninoriyah Isninoriyah
Inventory management of pharmaceutical supplies and medical consumables at pharmacies is very important to be considered, because management that is not according to procedures will result in inappropriate use, there will be many expired drugs and budget overlaps. The purpose of this study was to describe the management of pharmaceutical supplies and medical consumables at the Halim Medika Pharmacy Banjarbaru which includes planning, procurement, receipt, storage, expiration checks, control, destruction and withdrawal, recording and reporting. This research was a non-experimental. Data were collected using observation sheets based on the SOP of the Halim Medika Pharmacy and the Minister of Health Regulation No. 73, 2016. The sample in this study were all inventory management of pharmaceutical supplies and medical consumables at the Halim Medika Pharmacy Banjarbaru in February-March 2022. Data analysis was carried out by calculating the percentage of conformity. The results showed that 91% of the activities were appropriate, namely planning, procurement, and receiving activities, 9% were not appropriate, including storage and inspection activities for expiration and based on Permenkes No. 73/2016, 100% activities were in accordance with all, namely control activities, destruction and withdrawal, recording and reporting.
{"title":"GAMBARAN PENGELOLAAN PERSEDIAAN PERBEKALAN FARMASI DAN BAHAN MEDIS HABIS PAKAI DI APOTEK HALIM MEDIKA BANJARBARU","authors":"Amaliyah Wahyuni, Abdul Mahmud Yumassik, Isninoriyah Isninoriyah","doi":"10.36387/jifi.v6i1.1302","DOIUrl":"https://doi.org/10.36387/jifi.v6i1.1302","url":null,"abstract":"Inventory management of pharmaceutical supplies and medical consumables at pharmacies is very important to be considered, because management that is not according to procedures will result in inappropriate use, there will be many expired drugs and budget overlaps. The purpose of this study was to describe the management of pharmaceutical supplies and medical consumables at the Halim Medika Pharmacy Banjarbaru which includes planning, procurement, receipt, storage, expiration checks, control, destruction and withdrawal, recording and reporting. This research was a non-experimental. Data were collected using observation sheets based on the SOP of the Halim Medika Pharmacy and the Minister of Health Regulation No. 73, 2016. The sample in this study were all inventory management of pharmaceutical supplies and medical consumables at the Halim Medika Pharmacy Banjarbaru in February-March 2022. Data analysis was carried out by calculating the percentage of conformity. The results showed that 91% of the activities were appropriate, namely planning, procurement, and receiving activities, 9% were not appropriate, including storage and inspection activities for expiration and based on Permenkes No. 73/2016, 100% activities were in accordance with all, namely control activities, destruction and withdrawal, recording and reporting.","PeriodicalId":306551,"journal":{"name":"Jurnal Insan Farmasi Indonesia","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121220023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alda Safitri Maria Ulfa, Emelda Emelda, Muhammad Abdurrahman Munir, Nanik Sulistyani
Papaya plant (Carica papaya L.) is a plant of Caricaceae family. Papaya has many health benefits, such as papaya seeds (Carica papaya L.) which can be used as medicine. This research is purely experimental with a research design to determine effect of maceration and soxhletation extraction methods on the standardization of specific and non-specific parameters of papaya seeds (Carica papaya L.). The result show extraction method did not affect the standardization of specific parameters (total phenolic content) which showed no significant difference to the total phenolic content (standard gallic acid) as seen from the results of the analysis p sig 0.184 (p>0.05). The extraction method showed that there was a significant difference to the water content with a p sig of 0.046 (p <0.05). The total ash content and specific gravity showed a significant difference to the extraction method with a p sig 0.000 (p <0.05). The conclusion of this study is that there is an influence of maceration and soxhletation extraction methods on non-specific parameters, moisture content, total ash content and specific gravity. Meanwhile, there was no effect of soxhletation and maceration extraction methods on specific parameters total phenolic content.
番木瓜(Carica Papaya L.)是一种番木瓜科植物。木瓜有许多健康益处，如番木瓜种子(Carica Papaya L.)可以用作药物。本研究为纯实验研究，旨在确定浸渍法和浸提法对番木瓜种子特异性和非特异性参数标准化的影响。结果表明，提取方法不影响具体参数(总酚含量)的标准化，与总酚含量(标准没食子酸)的标准化差异无统计学意义(p sig 0.184 (p>0.05))。不同提取方法对水含量的影响差异显著(p <0.05)， p < 0.046。总灰分和比重与提取方法差异显著(p <0.05)， p < 0.000。本研究的结论是，浸渍和浸渍提取方法对非特异性参数、水分含量、总灰分含量和比重都有影响。同时，浸渍法和浸渍法对总酚含量的具体参数没有影响。
{"title":"PENGARUH METODE EKSTRAKSI MASERASI DAN SOKLETASI TERHADAP STANDARDISASI PARAMETER SPESIFIK DAN NON SPESIFIK EKSTRAK ETANOL BIJI PEPAYA (Carica papaya L.)","authors":"Alda Safitri Maria Ulfa, Emelda Emelda, Muhammad Abdurrahman Munir, Nanik Sulistyani","doi":"10.36387/jifi.v6i1.1387","DOIUrl":"https://doi.org/10.36387/jifi.v6i1.1387","url":null,"abstract":"Papaya plant (Carica papaya L.) is a plant of Caricaceae family. Papaya has many health benefits, such as papaya seeds (Carica papaya L.) which can be used as medicine. This research is purely experimental with a research design to determine effect of maceration and soxhletation extraction methods on the standardization of specific and non-specific parameters of papaya seeds (Carica papaya L.). The result show extraction method did not affect the standardization of specific parameters (total phenolic content) which showed no significant difference to the total phenolic content (standard gallic acid) as seen from the results of the analysis p sig 0.184 (p>0.05). The extraction method showed that there was a significant difference to the water content with a p sig of 0.046 (p <0.05). The total ash content and specific gravity showed a significant difference to the extraction method with a p sig 0.000 (p <0.05). The conclusion of this study is that there is an influence of maceration and soxhletation extraction methods on non-specific parameters, moisture content, total ash content and specific gravity. Meanwhile, there was no effect of soxhletation and maceration extraction methods on specific parameters total phenolic content.","PeriodicalId":306551,"journal":{"name":"Jurnal Insan Farmasi Indonesia","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122283427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fitriana Novita Sari, A. Indrayanti, Pudiastuti Rahayu Sawarno Putri
Free radicals can cause various kinds of degenerative diseases and skin aging. Free radicals can be neutralized with an enzymatic antioxidant, namely superoxide dismutase (SOD). One of the plants that has high SOD activity is salam (Syzygium polyanthum). The aim of this study was to determine the activity of the crude SOD extract of bay leaves precipitated with ammonium sulfate salt at various concentrations of 25, 50, 75%. The study began with plant determination, enzyme extraction, precipitation of enzyme extracts with ammonium sulfate concentrations of 25, 50, 75%, measurement of total protein content and SOD activity test using the WST-1 method to calculate the percent inhibition. The results were statistically analyzed using one way ANOVA. The results showed that the total protein content of the crude extract of SOD and the results of precipitation with ammonium sulfate 25, 50, and 75% respectively were 9.38; 6.36; 8.50; and 11.6 mg/mL. Meanwhile, the percentage inhibition values were 73.13; 34.33; 65.67; and 80.59%. The highest SOD activity was obtained in the crude extract of the enzyme precipitated with 75% ammonium sulfate salt, which was 80.59%.
{"title":"UJI AKTIVITAS ENZIM SUPEROKSIDA DISMUTASE (SOD) EKSTRAK DAUN SALAM (Syzygium polyanthum) DENGAN METODE WATER SOLUBLE TETRAZOLIUM SALT-1 (WST-1)","authors":"Fitriana Novita Sari, A. Indrayanti, Pudiastuti Rahayu Sawarno Putri","doi":"10.36387/jifi.v6i1.1270","DOIUrl":"https://doi.org/10.36387/jifi.v6i1.1270","url":null,"abstract":"Free radicals can cause various kinds of degenerative diseases and skin aging. Free radicals can be neutralized with an enzymatic antioxidant, namely superoxide dismutase (SOD). One of the plants that has high SOD activity is salam (Syzygium polyanthum). The aim of this study was to determine the activity of the crude SOD extract of bay leaves precipitated with ammonium sulfate salt at various concentrations of 25, 50, 75%. The study began with plant determination, enzyme extraction, precipitation of enzyme extracts with ammonium sulfate concentrations of 25, 50, 75%, measurement of total protein content and SOD activity test using the WST-1 method to calculate the percent inhibition. The results were statistically analyzed using one way ANOVA. The results showed that the total protein content of the crude extract of SOD and the results of precipitation with ammonium sulfate 25, 50, and 75% respectively were 9.38; 6.36; 8.50; and 11.6 mg/mL. Meanwhile, the percentage inhibition values were 73.13; 34.33; 65.67; and 80.59%. The highest SOD activity was obtained in the crude extract of the enzyme precipitated with 75% ammonium sulfate salt, which was 80.59%.","PeriodicalId":306551,"journal":{"name":"Jurnal Insan Farmasi Indonesia","volume":"60 s79","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120835737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wiwi Kusumasari, Ana Indrayati, Lukito Mindi Cahyo
Tofu waste that is discharged into the aquatic environment has a negative impact on the quality of the water around the tofu liquid waste disposal site. Industrial enzyme production is currently dominated by proteases. Proteases in the pharmaceutical field support the absorption of proteins in the digestive tract, as active cosmetic ingredients. This study aims to isolate, identify protease-producing bacteria from tofu wastewater, determine the total protein content and enzyme activity index of 5 isolates of protease-producing bacteria. Tofu wastewater bacteria were isolated with Skim Milk Agar medium. 5 bacterial isolates were selected which produced the widest clear zones, different colony characters, identified by morphological tests, gram staining tests, and biochemical tests. Five isolates were made into a bacterial suspension isolated from crude protease extract and the protease level was determined using the Bicinchoninic Acid method, and the protease activity was tested. Five bacterial colonies showed that colony 1 and colony 2 included Gram positive bacteria, colonies 3, 4 and 5 included Gram negative bacteria. The value of total protein content in the tofu industrial wastewater from the five colonies respectively: 219.93; 833,267; 421,267; 81,267; and 38.6 µg/µL. The highest proteolytic activity test was in sample 2 of 2.13.
{"title":"AKTIVITAS PROTEASE EKSTRASELULER BAKTERI YANG DIISOLASI DARI LIMBAH CAIR INDUSTRI TAHU","authors":"Wiwi Kusumasari, Ana Indrayati, Lukito Mindi Cahyo","doi":"10.36387/jifi.v6i1.1266","DOIUrl":"https://doi.org/10.36387/jifi.v6i1.1266","url":null,"abstract":"Tofu waste that is discharged into the aquatic environment has a negative impact on the quality of the water around the tofu liquid waste disposal site. Industrial enzyme production is currently dominated by proteases. Proteases in the pharmaceutical field support the absorption of proteins in the digestive tract, as active cosmetic ingredients. This study aims to isolate, identify protease-producing bacteria from tofu wastewater, determine the total protein content and enzyme activity index of 5 isolates of protease-producing bacteria. Tofu wastewater bacteria were isolated with Skim Milk Agar medium. 5 bacterial isolates were selected which produced the widest clear zones, different colony characters, identified by morphological tests, gram staining tests, and biochemical tests. Five isolates were made into a bacterial suspension isolated from crude protease extract and the protease level was determined using the Bicinchoninic Acid method, and the protease activity was tested. Five bacterial colonies showed that colony 1 and colony 2 included Gram positive bacteria, colonies 3, 4 and 5 included Gram negative bacteria. The value of total protein content in the tofu industrial wastewater from the five colonies respectively: 219.93; 833,267; 421,267; 81,267; and 38.6 µg/µL. The highest proteolytic activity test was in sample 2 of 2.13.","PeriodicalId":306551,"journal":{"name":"Jurnal Insan Farmasi Indonesia","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115139452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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