It would seem that today the most important thing for many individuals is to have a good profile picture on online social networks. Accordingly, there is a serious possibility that addiction to social networking sites (SNS) can lead to psychosocial and mental disorders and other negative consequences on health-related issues. So, the aim of this study was to determine the association between the excessive use of social networking sites with the symptoms of depression and anxiety. This cross-sectional study was carried out at the University of Niš, Faculty of Medicine (Serbia) from September to December 2019. The study included 1,405 participants and all of them were assessed by using an appropriate questionnaire. The statistical analysis of the data included the binary logistic regression. The strongest predictor of whether one has high levels of depression symptoms was the state of conflict where the odds ratio (OR) was 10.28 (95% CI: 5.84 — 18.08). It has been observed that depression symptoms are also more common in those respondents with expressed withdrawal symptoms (OR = 4.27, 95% CI: 3.04 — 6.00), and with mood modification (OR = 1.82, 95% CI: 1.12 — 2.96). Anxiety symptoms, similar to those of depression, are most present in individuals with conflict state (OR = 10.31, 95% CI: 5.25 — 20.26), in those with withdrawal symptoms (OR = 6.83, 95% CI: 4.98 — 9.38), and then with mood modification (OR = 2.55, 95% CI: 1.60 — 4.07). The number of active accounts/profiles and number of devices, as well as state of salience and tolerance played no part in any of the cases. This study has shown a direct link between excessive SNS use and depression and anxiety symptoms. Further detailed research, especially longitudinal studies, is needed to understand the essential nature of these links. In any case, public health professionals should draw the attention of people, especially the younger population, in terms of limiting the use of social networks. Acta Medica Medianae 2021;60(1):28-37.
{"title":"THE ASSOCIATION BETWEEN DEPRESSION AND ANXIETY SYMPTOMS WITH SIX CORE COMPONENTS OF SOCIAL NETWORKING SITES ADDICTION: A CROSS-SECTIONAL STUDY IN SERBIA","authors":"T. Jovanović, A. Višnjić, Marko Gmijović","doi":"10.5633/AMM.2021.0104","DOIUrl":"https://doi.org/10.5633/AMM.2021.0104","url":null,"abstract":"It would seem that today the most important thing for many individuals is to have a good profile picture on online social networks. Accordingly, there is a serious possibility that addiction to social networking sites (SNS) can lead to psychosocial and mental disorders and other negative consequences on health-related issues. So, the aim of this study was to determine the association between the excessive use of social networking sites with the symptoms of depression and anxiety. This cross-sectional study was carried out at the University of Niš, Faculty of Medicine (Serbia) from September to December 2019. The study included 1,405 participants and all of them were assessed by using an appropriate questionnaire. The statistical analysis of the data included the binary logistic regression. The strongest predictor of whether one has high levels of depression symptoms was the state of conflict where the odds ratio (OR) was 10.28 (95% CI: 5.84 — 18.08). It has been observed that depression symptoms are also more common in those respondents with expressed withdrawal symptoms (OR = 4.27, 95% CI: 3.04 — 6.00), and with mood modification (OR = 1.82, 95% CI: 1.12 — 2.96). Anxiety symptoms, similar to those of depression, are most present in individuals with conflict state (OR = 10.31, 95% CI: 5.25 — 20.26), in those with withdrawal symptoms (OR = 6.83, 95% CI: 4.98 — 9.38), and then with mood modification (OR = 2.55, 95% CI: 1.60 — 4.07). The number of active accounts/profiles and number of devices, as well as state of salience and tolerance played no part in any of the cases. This study has shown a direct link between excessive SNS use and depression and anxiety symptoms. Further detailed research, especially longitudinal studies, is needed to understand the essential nature of these links. In any case, public health professionals should draw the attention of people, especially the younger population, in terms of limiting the use of social networks. Acta Medica Medianae 2021;60(1):28-37.","PeriodicalId":31409,"journal":{"name":"Acta Medica Medianae","volume":"60 1","pages":"28-37"},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44870541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE EFFECT OF OCCUPATIONAL STRESSORS IN PUBLIC COMPANIES ON TEMPORARY AND PERMANENT WORKING ABILITY OF WORKERS WITH ARTERIAL HYPERTENSION","authors":"Blerim Çupi","doi":"10.5633/AMM.2021.0108","DOIUrl":"https://doi.org/10.5633/AMM.2021.0108","url":null,"abstract":"","PeriodicalId":31409,"journal":{"name":"Acta Medica Medianae","volume":"60 1","pages":"63-67"},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48389799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The new coronavirus SARS-CoV-2 has quickly spread all over the globe causing a global pandemic The intrinsic properties of the virus make it potentially fatal, as COVID-19 disease severity varies among individuals with currently unknown background The world community is thus in a desperate need for novel antiviral drugs against SARS-CoV-2 In this work, we focus on macromolecular targets present on SARS-CoV-2 virus as potential targets for antiviral drugs In the continuation, we offer a brief presentation of drugs or drug candidates that act directly on virus life cycle and have promising effects in COVID-19 therapy (English) [ABSTRACT FROM AUTHOR] Novi koronavirus SARS-CoV-2 brzo se prosirio svetom, uzrokujuci globalnu pandemiju Karakteristicna svojstva virusa cine ga potencijalno fatalnim, jer težina bolesti COVID-19 varira među osobama, sa trenutno nepoznatom pozadinom Svetska zajednica stoga ocajnicki traga za novim antivirusnim lekovima protiv SARS-CoV-2 virusa U ovom radu, fokusiramo se na makromolekularne ciljeve prisutne na virusu SARS-CoV-2, kao i na potencijalne ciljeve za antivirusne lekove U nastavku nudimo kratku prezentaciju lekova ili kandidata za lekove, koji deluju direktno na životni ciklus virusa i imaju obecavajuce efekte u terapiji COVID-19 bolesti (Bosnian) [ABSTRACT FROM AUTHOR] Copyright of Acta Medica Medianae is the property of Acta Medica Medianae and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission However, users may print, download, or email articles for individual use This abstract may be abridged No warranty is given about the accuracy of the copy Users should refer to the original published version of the material for the full abstract (Copyright applies to all Abstracts )
新型冠状病毒SARS-CoV-2在全球范围内迅速传播，导致全球大流行，由于COVID-19疾病在目前背景未知的个体中严重程度不同，该病毒的内在特性使其具有潜在的致命性，因此国际社会迫切需要针对SARS-CoV-2的新型抗病毒药物。在本工作中，我们将重点关注SARS-CoV-2病毒存在的大分子靶点作为抗病毒药物的潜在靶点。新冠病毒SARS-CoV-2 brzo - se prosirio system, uzrokujuci globalnu pandemiju Karakteristicna svojstva virusa cine ga potenticijalno fatalm, jer težina bolesti COVID-19 varira među osobama，原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译:原译[摘要]未经版权所有人的明确书面许可，版权归Acta Medica Medianae所有，不得将其内容复制、通过电子邮件发送到多个网站或发布到某个列表服务器。本摘要可能被删节，不保证副本的准确性，用户应参考材料的原始出版版本(版权适用于所有摘要)
{"title":"OVERVIEW OF ANTIVIRAL DRUGS AGAINST SARS-CoV-2 FOR THE TREATMENT OF COVID-19","authors":"M. Anderluh, T. Tomašič, Ž. Jakopin","doi":"10.5633/AMM.2021.0101","DOIUrl":"https://doi.org/10.5633/AMM.2021.0101","url":null,"abstract":"The new coronavirus SARS-CoV-2 has quickly spread all over the globe causing a global pandemic The intrinsic properties of the virus make it potentially fatal, as COVID-19 disease severity varies among individuals with currently unknown background The world community is thus in a desperate need for novel antiviral drugs against SARS-CoV-2 In this work, we focus on macromolecular targets present on SARS-CoV-2 virus as potential targets for antiviral drugs In the continuation, we offer a brief presentation of drugs or drug candidates that act directly on virus life cycle and have promising effects in COVID-19 therapy (English) [ABSTRACT FROM AUTHOR] Novi koronavirus SARS-CoV-2 brzo se prosirio svetom, uzrokujuci globalnu pandemiju Karakteristicna svojstva virusa cine ga potencijalno fatalnim, jer težina bolesti COVID-19 varira među osobama, sa trenutno nepoznatom pozadinom Svetska zajednica stoga ocajnicki traga za novim antivirusnim lekovima protiv SARS-CoV-2 virusa U ovom radu, fokusiramo se na makromolekularne ciljeve prisutne na virusu SARS-CoV-2, kao i na potencijalne ciljeve za antivirusne lekove U nastavku nudimo kratku prezentaciju lekova ili kandidata za lekove, koji deluju direktno na životni ciklus virusa i imaju obecavajuce efekte u terapiji COVID-19 bolesti (Bosnian) [ABSTRACT FROM AUTHOR] Copyright of Acta Medica Medianae is the property of Acta Medica Medianae and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission However, users may print, download, or email articles for individual use This abstract may be abridged No warranty is given about the accuracy of the copy Users should refer to the original published version of the material for the full abstract (Copyright applies to all Abstracts )","PeriodicalId":31409,"journal":{"name":"Acta Medica Medianae","volume":"60 1","pages":"5-12"},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45672215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Cvetanovic, N. Zivkovic, M. Kostic, Miljana Džunić, B. Jovanovic
{"title":"ESTROGEN RECEPTOR AS A RESISTANCE MECHANISM TO TRASTUZUMAB","authors":"A. Cvetanovic, N. Zivkovic, M. Kostic, Miljana Džunić, B. Jovanovic","doi":"10.5633/AMM.2021.0105","DOIUrl":"https://doi.org/10.5633/AMM.2021.0105","url":null,"abstract":"","PeriodicalId":31409,"journal":{"name":"Acta Medica Medianae","volume":"60 1","pages":"38-47"},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44368143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kostic, Tatijana Tošić, R. Mitić, M. Radisavljevic, P. Milosevic, Slavko Živković, B. Stanojević
Here we present a case of a 10-year-old girl who was diagnosed with SWS at birth. The history of seizures and transient right-sided hemiparesis dates from the neonatal period. Portwine stains on the face, right hand and foot were present from the birth of the child which may suggest overlapping with KTS. When the girl suffered mild head trauma, some hours after, vomiting, aphasia, and hemiplegia occurred. The patient was admitted to the Clinic of Neurosurgery and after observation, she was commenced on symptomatic and antiepileptic therapy. Within the following 24 hours, aphasia resolved and hemiplegia turned into mild hemiparesis. As the neurological status improved and the patient felt better, after two more days of observation, the child was discharged from the hospital. Acta Medica Medianae 2021;60(1):74-78.
{"title":"HEAD TRAUMA IN A CHILD WITH STURGE-WEBER SYNDROME OVERLAPPING KLIPPEL-TRENAUNAY SYNDROME","authors":"A. Kostic, Tatijana Tošić, R. Mitić, M. Radisavljevic, P. Milosevic, Slavko Živković, B. Stanojević","doi":"10.5633/AMM.2021.0110","DOIUrl":"https://doi.org/10.5633/AMM.2021.0110","url":null,"abstract":"Here we present a case of a 10-year-old girl who was diagnosed with SWS at birth. The history of seizures and transient right-sided hemiparesis dates from the neonatal period. Portwine stains on the face, right hand and foot were present from the birth of the child which may suggest overlapping with KTS. When the girl suffered mild head trauma, some hours after, vomiting, aphasia, and hemiplegia occurred. The patient was admitted to the Clinic of Neurosurgery and after observation, she was commenced on symptomatic and antiepileptic therapy. Within the following 24 hours, aphasia resolved and hemiplegia turned into mild hemiparesis. As the neurological status improved and the patient felt better, after two more days of observation, the child was discharged from the hospital. Acta Medica Medianae 2021;60(1):74-78.","PeriodicalId":31409,"journal":{"name":"Acta Medica Medianae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47989299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ovarian cancer represents the third most common gynecological cancer worldwide, and the most lethal one. The aim of the study was to assess ovarian cancer incidence trend in the Nišava District in the period 1999-2017. Descriptive epidemiological method was applied. Data used for analyses were provided by the Cancer Register of the Institute of Public Health Niš and from the Serbian Cancer Registry. Incidence trends were based on crude and age-standardized incidence rates, calculated by the direct method of standardization per 100,000. Ovarian cancer represented 5.1% of all new registered malignant diseases in females with a total of 791 new cases. There were no cases of ovarian cancer registered in females before the age of 15. A statistically significant increasing trend with aging was registered. The average annual crude incidence rate was 22.0/100,000, while the average age-standardized incidence rate was 13.3/100,000. The highest age-standardized incidence rate was registered in 2016 (20.4), while the lowest rate was reported in 2002 (10.1). Ovarian cancer incidence trend, based on crude incidence rates, as well as on age-standardized incidence rates, increased. The highest age-adjusted incidence rates were in the municipalities of Svrljig (18.4) and Gadžin Han (14.0) and the lowest in the municipalities of Merošina (7.4) and Doljevac (9.0). Registration of unfavorable incidence trends of the disease indicate failure in the primary and secondary prevention in the past. It is of vital importance to provide more intensive and comprehensive activities that may lead to the reduction of risk factors and to early detection of the disease. Acta Medica Medianae 2021;60(1):13-18.
{"title":"OVARIAN CANCER INCIDENCE TREND IN THE NIŠAVA DISTRICT","authors":"B. Kocić, N. Rancic, Mirko V Ilić, I. Kocić","doi":"10.5633/AMM.2021.0102","DOIUrl":"https://doi.org/10.5633/AMM.2021.0102","url":null,"abstract":"Ovarian cancer represents the third most common gynecological cancer worldwide, and the most lethal one. The aim of the study was to assess ovarian cancer incidence trend in the Nišava District in the period 1999-2017. Descriptive epidemiological method was applied. Data used for analyses were provided by the Cancer Register of the Institute of Public Health Niš and from the Serbian Cancer Registry. Incidence trends were based on crude and age-standardized incidence rates, calculated by the direct method of standardization per 100,000. Ovarian cancer represented 5.1% of all new registered malignant diseases in females with a total of 791 new cases. There were no cases of ovarian cancer registered in females before the age of 15. A statistically significant increasing trend with aging was registered. The average annual crude incidence rate was 22.0/100,000, while the average age-standardized incidence rate was 13.3/100,000. The highest age-standardized incidence rate was registered in 2016 (20.4), while the lowest rate was reported in 2002 (10.1). Ovarian cancer incidence trend, based on crude incidence rates, as well as on age-standardized incidence rates, increased. The highest age-adjusted incidence rates were in the municipalities of Svrljig (18.4) and Gadžin Han (14.0) and the lowest in the municipalities of Merošina (7.4) and Doljevac (9.0). Registration of unfavorable incidence trends of the disease indicate failure in the primary and secondary prevention in the past. It is of vital importance to provide more intensive and comprehensive activities that may lead to the reduction of risk factors and to early detection of the disease. Acta Medica Medianae 2021;60(1):13-18.","PeriodicalId":31409,"journal":{"name":"Acta Medica Medianae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43941347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Katarina Danković, N. Stefanović, R. Veličković-Radovanović, B. Mitić, G. Paunović, M. Cvetković, T. Cvetković
Bradykinin (BK), a vasoactive peptide, is considered to exert the renoprotective and cardioprotective effects. Furthermore, most of its effects are mediated by the activation of BK type 2 receptor (B2R), whose level of expression is influenced by insertion/deletion (+9/-9) gene polymorphism. The aim of this study was to investigate the potential influence of B2R +9/-9 gene polymorphism on kidney function and blood pressure in kidney transplant recipients (KTRs) within the first post-transplantation year. Secondary, the study analyzed the correlation between hypertensive status and graft function, with respect to the B2R +9/-9 gene polymorphism. The pharmacogenetic study included 95 KTRs with clinical and biochemical parameters evaluated in three time-points (3, 6, 12 month). The graft function was assessed using the estimated glomerular filtration rate (eGFR), while systolic (SBP), diastolic (DBP), and mean blood pressure (MAP) were used as parameters for the evaluation of blood pressure control. The carriers of -9/-9 genotype had more stable graft function compared to both, heterozygotes and homozygous for +9 allele, not only in the early (up to 6 months) but as well in the late post-transplantation period (beyond 6 months). Also, the -9/-9 genotype carriers showed a tendency to significantly decrease MAP, SBP, and DBP in the observed period, whereby only in this patient group the significant association between hypertensive status and graft function was demonstrated. In conclusion, genotyping of B2R +9/-9 gene polymorphism alongside with biochemical and clinical parameters may identify patients with respect to the risk of more prompt decline of graft function. Acta Medica Medianae 2021;60(1):19-27.
{"title":"POTENTIAL SIGNIFICANCE OF BRADYKININ 2 RECEPTOR GENOTYPING IN KIDNEY TRANSPLANT RECIPIENTS","authors":"Katarina Danković, N. Stefanović, R. Veličković-Radovanović, B. Mitić, G. Paunović, M. Cvetković, T. Cvetković","doi":"10.5633/AMM.2021.0103","DOIUrl":"https://doi.org/10.5633/AMM.2021.0103","url":null,"abstract":"Bradykinin (BK), a vasoactive peptide, is considered to exert the renoprotective and cardioprotective effects. Furthermore, most of its effects are mediated by the activation of BK type 2 receptor (B2R), whose level of expression is influenced by insertion/deletion (+9/-9) gene polymorphism. The aim of this study was to investigate the potential influence of B2R +9/-9 gene polymorphism on kidney function and blood pressure in kidney transplant recipients (KTRs) within the first post-transplantation year. Secondary, the study analyzed the correlation between hypertensive status and graft function, with respect to the B2R +9/-9 gene polymorphism. The pharmacogenetic study included 95 KTRs with clinical and biochemical parameters evaluated in three time-points (3, 6, 12 month). The graft function was assessed using the estimated glomerular filtration rate (eGFR), while systolic (SBP), diastolic (DBP), and mean blood pressure (MAP) were used as parameters for the evaluation of blood pressure control. The carriers of -9/-9 genotype had more stable graft function compared to both, heterozygotes and homozygous for +9 allele, not only in the early (up to 6 months) but as well in the late post-transplantation period (beyond 6 months). Also, the -9/-9 genotype carriers showed a tendency to significantly decrease MAP, SBP, and DBP in the observed period, whereby only in this patient group the significant association between hypertensive status and graft function was demonstrated. In conclusion, genotyping of B2R +9/-9 gene polymorphism alongside with biochemical and clinical parameters may identify patients with respect to the risk of more prompt decline of graft function. Acta Medica Medianae 2021;60(1):19-27.","PeriodicalId":31409,"journal":{"name":"Acta Medica Medianae","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70776478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Veljković, Jovan Hadži-Đokić, G. Stanojevic, B. Brankovic, A. Skakić, Ivana Damjanović, Mladen Stojanović, A. Šmelcerović, G. Kocić
Colorectal cancer (CRC) is one of the main reasons for the mortality connected with tumor diseases. There is still a shortage of examination including the influence of urate pathway enzymes in the progressiveness and invasion of CRC, so the present study investigated the role of xanthine oxidase (XO), adenosine deaminase (ADA) and 5'-nucleotidase (5'-NT) activity, concerning TBA-reactive substances (TBARS) as an oxidative stress (OS) marker in progression, also an invasion of human colorectal cancer. We took tissue specimens from 50 patients with colon cancer, in all four TNM clinical stages of the disease. They were divided into 3 groups: cancer tissue, tissue surrounding the tumor and healthy control tissue group. We made 10% homogenates in which we conducted the study with proper methods. The activity of ADA and XO in tumor tissue and tissue adjacent to the tumor is statistically higher in comparison to healthy colon tissue. The 5'-NT is not significantly higher in carcinoma tissue. The highest activity of ADA and XO is in T2 and T3 tumor stages. TBARS has the highest concentration in T3 and T4 stages of the tumor. Presented results suggest that the possible cause of OS in colon carcinoma is high XO and ADA activity. It may include those enzymes in the transformation of the colon tissue, as well as in the progression of CRC. So, the ADA and XO activity might be helpful in determing the margins of colon resection. They can have significance in diagnosis, but in the prognosis of the disease as well.
{"title":"The importance of urate pathway enzymes activity and its relation with oxidative stress in progression and invasion of human colorectal cancer","authors":"A. Veljković, Jovan Hadži-Đokić, G. Stanojevic, B. Brankovic, A. Skakić, Ivana Damjanović, Mladen Stojanović, A. Šmelcerović, G. Kocić","doi":"10.5633/AMM.2021.0201","DOIUrl":"https://doi.org/10.5633/AMM.2021.0201","url":null,"abstract":"Colorectal cancer (CRC) is one of the main reasons for the mortality connected with tumor diseases. There is still a shortage of examination including the influence of urate pathway enzymes in the progressiveness and invasion of CRC, so the present study investigated the role of xanthine oxidase (XO), adenosine deaminase (ADA) and 5'-nucleotidase (5'-NT) activity, concerning TBA-reactive substances (TBARS) as an oxidative stress (OS) marker in progression, also an invasion of human colorectal cancer. We took tissue specimens from 50 patients with colon cancer, in all four TNM clinical stages of the disease. They were divided into 3 groups: cancer tissue, tissue surrounding the tumor and healthy control tissue group. We made 10% homogenates in which we conducted the study with proper methods. The activity of ADA and XO in tumor tissue and tissue adjacent to the tumor is statistically higher in comparison to healthy colon tissue. The 5'-NT is not significantly higher in carcinoma tissue. The highest activity of ADA and XO is in T2 and T3 tumor stages. TBARS has the highest concentration in T3 and T4 stages of the tumor. Presented results suggest that the possible cause of OS in colon carcinoma is high XO and ADA activity. It may include those enzymes in the transformation of the colon tissue, as well as in the progression of CRC. So, the ADA and XO activity might be helpful in determing the margins of colon resection. They can have significance in diagnosis, but in the prognosis of the disease as well.","PeriodicalId":31409,"journal":{"name":"Acta Medica Medianae","volume":"60 1","pages":"5-14"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70776524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Kundalic, Vesna Stojanovic, Miljana Pavlović, V. Zivkovic, M. Trandafilović, Jovana Čukuranović-Kokoris, Milorad Antić, I. Graovac
Based on the pathohistological examination of tonsillar tissue, chronic tonsillitis can be classified as chronic hypertrophic tonsillitis (CHT) and recurrent tonsillitis (RT). CD68 is a glycoprotein ubiquitously expressed on the cells of the monocyte-macrophage lineage, as well as on the dendritic cells. Macrophages and dendritic cells are major initiators, effectors, and regulators of immune response in the palatine tonsil. The aim of this paper was to examine microanatomical distribution of CD68-immunopositive cells and to determine their numerical areal density in morphological compartments of palatine tonsils with CHT and RT, in order to show the possible differences in antigen-presentation potential between these two pathological conditions. As a material we used tonsils taken after tonsillectomy, from patients of both sexes, aged 10-29 years: six tonsils with RT and nine tonsils with CHT. The quantification of CD68immunopositive cells by "ImageJ" software was performed on 5 µm thick serial paraffin tissue slices, which were stained immunohistochemically, by using monoclonal anti-CD68 antibody. The results of morphometrical analysis showed presence of CD68-immunopositive cells in all morphological compartments of tonsils with RT and CHT, being higher in number in RT compared with CHT. Statistically significant difference in numerical areal density of the CD68immunopositive cells was found in the germinal centers of lymphoid follicles (RT > CHT), and interfollicular lymphoid tissue (CHT > RT). The difference in the number of CD68-immunopositive cells might imply the different mechanisms involved in the infiltration of tonsillar tissue with CD68-immunopositive cells, as well as the different antigen-presenting potential in these two conditions.
{"title":"Expression of CD68 antigen in chronically diseased human palatine tonsil","authors":"B. Kundalic, Vesna Stojanovic, Miljana Pavlović, V. Zivkovic, M. Trandafilović, Jovana Čukuranović-Kokoris, Milorad Antić, I. Graovac","doi":"10.5633/AMM.2021.0206","DOIUrl":"https://doi.org/10.5633/AMM.2021.0206","url":null,"abstract":"Based on the pathohistological examination of tonsillar tissue, chronic tonsillitis can be classified as chronic hypertrophic tonsillitis (CHT) and recurrent tonsillitis (RT). CD68 is a glycoprotein ubiquitously expressed on the cells of the monocyte-macrophage lineage, as well as on the dendritic cells. Macrophages and dendritic cells are major initiators, effectors, and regulators of immune response in the palatine tonsil. The aim of this paper was to examine microanatomical distribution of CD68-immunopositive cells and to determine their numerical areal density in morphological compartments of palatine tonsils with CHT and RT, in order to show the possible differences in antigen-presentation potential between these two pathological conditions. As a material we used tonsils taken after tonsillectomy, from patients of both sexes, aged 10-29 years: six tonsils with RT and nine tonsils with CHT. The quantification of CD68immunopositive cells by \"ImageJ\" software was performed on 5 µm thick serial paraffin tissue slices, which were stained immunohistochemically, by using monoclonal anti-CD68 antibody. The results of morphometrical analysis showed presence of CD68-immunopositive cells in all morphological compartments of tonsils with RT and CHT, being higher in number in RT compared with CHT. Statistically significant difference in numerical areal density of the CD68immunopositive cells was found in the germinal centers of lymphoid follicles (RT > CHT), and interfollicular lymphoid tissue (CHT > RT). The difference in the number of CD68-immunopositive cells might imply the different mechanisms involved in the infiltration of tonsillar tissue with CD68-immunopositive cells, as well as the different antigen-presenting potential in these two conditions.","PeriodicalId":31409,"journal":{"name":"Acta Medica Medianae","volume":"60 1","pages":"51-56"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70776544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Daniela Benedeto-Stojanov, G. Koraćević, D. Stojanov, M. Koraćević, N. Ignjatovic
Proton pump inhibitors (PPIs) are the most potent drugs for suppressing gastric acid secretion. They are used in the treatment of acid-peptic disorders, including peptic ulcer disease, gastroesophageal reflux disease, Zollinger Ellison syndrome, in the eradication of Helicobacter pylori infection and ulcer prophylaxis. In the pharmacotherapy of these disorders, they have significantly suppressed the use of H2 blockers, like other, older groups of antisecretory drugs. Long-term PPI therapy leads to moderate hypergastrinemia (increased gastrin secretion) in 20-25% of patients. This hypergastrinemia results in rebound acid hypersecretion (RAHS) in 30-40% patients, who abruptly discontinue PPI. Most patients who abruptly discontinue PPI have symptoms of dyspepsia and gastroesophageal reflux, most commonly heartburn and a burning sensation in the esophagus. Therefore, care should be taken to properly discontinue PPI and reduce the dose of the drug before complete discontinuation. A less effective acid blocker (H2 blocker) can be switched, since H2 receptor blockers cause less pronounced hypergastrinemia and hyperplasia of enterochromaffin-like cells (ECL cells) compared to PPI.
{"title":"Rebound phenomenon of proton pump inhibitor therapy","authors":"Daniela Benedeto-Stojanov, G. Koraćević, D. Stojanov, M. Koraćević, N. Ignjatovic","doi":"10.5633/AMM.2021.0208","DOIUrl":"https://doi.org/10.5633/AMM.2021.0208","url":null,"abstract":"Proton pump inhibitors (PPIs) are the most potent drugs for suppressing gastric acid secretion. They are used in the treatment of acid-peptic disorders, including peptic ulcer disease, gastroesophageal reflux disease, Zollinger Ellison syndrome, in the eradication of Helicobacter pylori infection and ulcer prophylaxis. In the pharmacotherapy of these disorders, they have significantly suppressed the use of H2 blockers, like other, older groups of antisecretory drugs. Long-term PPI therapy leads to moderate hypergastrinemia (increased gastrin secretion) in 20-25% of patients. This hypergastrinemia results in rebound acid hypersecretion (RAHS) in 30-40% patients, who abruptly discontinue PPI. Most patients who abruptly discontinue PPI have symptoms of dyspepsia and gastroesophageal reflux, most commonly heartburn and a burning sensation in the esophagus. Therefore, care should be taken to properly discontinue PPI and reduce the dose of the drug before complete discontinuation. A less effective acid blocker (H2 blocker) can be switched, since H2 receptor blockers cause less pronounced hypergastrinemia and hyperplasia of enterochromaffin-like cells (ECL cells) compared to PPI.","PeriodicalId":31409,"journal":{"name":"Acta Medica Medianae","volume":"60 1","pages":"64-68"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70776589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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