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해양 종속영양세균의 한천대사에 대한 새로운 통찰 对海洋从属营养细菌闲天代谢的新洞察
Pub Date : 2022-12-31 DOI: 10.33147/lsnrr.2022.30.1.53
Pathiraja Mudiyanselage Duleepa Pathiraja, In-Geol Choi
Agar is a key structural polysaccharide of red macroalgae which provides a rich habitat for marine heterotrophic bacteria in marine ecosystems. Marine agarolytic bacteria, that can use agar as the sole carbon source, are distributed in diverse marine environments from open coastal waters to the gut of marine herbivores. Agarolytic bacteria employ a combination of carbohydrate-active enzymes (CAZymes) for the depolymerization of agar. Extensive studies on the genomic architecture of the agarolytic bacteria suggested that genes encoding these CAZymes are arranged in polysaccharide utilization loci (PUL). Agar hydrolyzing enzymes (agarases) are categorized into; β-agarase (GH16, GH50, GH86, and GH118), α-agarase (GH96), neoagarooligosaccharide hydrolase (GH117), and agarolytic β-galactosidase (GH2). The molecular functionality, structural elements, and catalytic mechanisms of agarases belonging to different GH families show unique characteristics. L-AHG, one of the main constituents in agar, is a rare monosaccharide and its metabolic pathway is exclusively found in marine agarolytic bacteria. Recent trends in the agarolytic systems are mostly focused on the sequence data to visualize the universal agarolytic enzyme repertoire and the evolution of the agarolytic pathway in marine heterotrophic bacteria. In addition, increasing attention is paid to understanding the oligosaccharide transport mechanisms and transcriptional regulation of genes in PUL. In this review, we will cover a comprehensive overview of genomic architecture, structural and functional analysis of agar hydrolyzing enzymes, and agar metabolism in marine heterotrophic bacteria.
琼脂是红藻的关键结构多糖,为海洋生态系统中的海洋异养细菌提供了丰富的栖息地。海洋溶脂菌以琼脂为唯一碳源,分布在从沿海开阔水域到海洋食草动物肠道的各种海洋环境中。溶脂细菌使用碳水化合物活性酶(CAZymes)的组合来解聚琼脂。对溶脂菌基因组结构的广泛研究表明,编码这些酶的基因位于多糖利用位点(PUL)上。琼脂水解酶(agarases)分为;β-琼脂酶(GH16、GH50、GH86和GH118)、α-琼脂酶(GH96)、新琼脂寡糖水解酶(GH117)和水解琼脂酶(GH2)。不同生长激素家族的琼脂酶在分子功能、结构成分和催化机理等方面表现出独特的特点。L-AHG是琼脂的主要成分之一,是一种罕见的单糖,其代谢途径仅存在于海洋溶脂菌中。近年来,海洋异养细菌对琼脂酶的研究主要集中在序列数据上,以直观地显示普遍的琼脂酶库和琼脂酶降解途径的演变。此外,人们越来越关注PUL中低聚糖的转运机制和基因的转录调控。在这篇综述中,我们将全面介绍基因组结构,琼脂水解酶的结构和功能分析,以及海洋异养细菌的琼脂代谢。
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引用次数: 0
대표적인 NADPH oxidase 저해제: 저해 방법, 효능, 그리고 다양한 질병에 대한 쓰임새 具有代表性的NADPH oxidase阻滞剂:阻抗方法、功效以及对多种疾病的应用
Pub Date : 2022-12-31 DOI: 10.33147/lsnrr.2022.30.1.33
Se-Hoon Shim, Yongseok Choi
NADPH Oxidase(NOX) induced reactive oxygen species(ROS) generation found on several types of cells including in leukocytes, artery wall, and brain. The human isoform complex comprises NOX1, NOX2, NOX3, NOX4, NOX5, DUOX1 and DUOX2. Each NOX isoforms consists of multi-subunit complex including membrane-associated enzymes and cytosolic subunits. NOX activation is step-by-step process where cytosolic subunits are assembled and translocated to the membrane, leading to transport electrons to oxygen, and finally to produce superoxide. Abnormal production of superoxide via NOX has been shown to play an important role in various diseases related to inflammation, cell growth, and progressive degenerative in cells. So, developing NOX inhibitors has been thought to be promising treatment and published many small molecules. This review will focus on representative NOX inhibitors with their chemical properties, potency on NOX, mechanisms, and limitation of use as a drug.
NADPH氧化酶(NOX)诱导活性氧(ROS)的产生,在几种类型的细胞中发现,包括白细胞、动脉壁和脑。人类异构体复合体包括NOX1、NOX2、NOX3、NOX4、NOX5、DUOX1和DUOX2。每种氮氧化物异构体由多亚基复合物组成,包括膜相关酶和细胞质亚基。氮氧化物活化是一个循序渐进的过程,其中细胞质亚基被组装并转移到膜上,导致电子传递到氧气,最终产生超氧化物。通过NOX异常产生超氧化物已被证明在与炎症、细胞生长和细胞进行性退行性疾病相关的各种疾病中发挥重要作用。因此,开发NOX抑制剂被认为是有前途的治疗方法,并发表了许多小分子。本文将重点介绍具有代表性的氮氧化物抑制剂的化学性质、对氮氧化物的抑制作用、作用机制以及作为药物使用的局限性。
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引用次数: 0
Leaching Behavior of Metallic Elements from Abandoned Mine Sites in Varying Environmental Factors 不同环境因素下废矿区金属元素的浸出行为
Pub Date : 2022-12-31 DOI: 10.33147/lsnrr.2022.30.1.87
Younji Lee, Minseok Park, Seunghun Hyun
Improper management of abandoned mine sites can cause heavy metal contamination of down-gradient agricultural soil, groundwater, and surface water. In this study, we investigated the effects of changes in environmental factors on the leaching behavior of the heavy metals and suggested future research direction for better risk management of the abandoned mine sites under climate change. Numerous studies showed that the concentration of heavy metals in the leachate frequently exceed the environmental criteria even though the leachable heavy metal was negligible (< 1 % of the total concentration), which indicates that mine soils can be long-term resources of contamination. From the result of batch and column experiments, it has been found that soil properties (e.g., soil pH, redox potential, etc.) affect the leaching behavior by solubility change, complex formation, precipitation, etc. Two-site kinetic leaching models were mainly applied for understanding nonequilibrium leaching behavior of the metals due to heterogeneous physical structure and various binding sites of the soil. Meanwhile, it was also reported that the change in hydrodynamic properties due to rainfall pattern can influence leaching behavior by changing the interfacial interaction between soil and water. Several researchers recognized that extreme weather condition (high temperature and increased drought period) due to climate change can elevate initial leaching concentration of the heavy metal. In reality, climate change can cause the nonequilibrium leaching of the metals by influencing hydrodynamic condition and chemical stability of the soil system. Therefore, future works to precisely predict the heavy metal leaching behavior across abandoned mine sites are necessary and risk management of these sites in response to climate change should be designed.
矿山废弃地管理不当,会对下坡农业土壤、地下水和地表水造成重金属污染。本研究探讨了环境因素变化对重金属浸出行为的影响,并提出了气候变化下废弃矿区风险管理的未来研究方向。大量研究表明,即使可浸出的重金属可以忽略不计(<总浓度的1%),但渗滤液中重金属的浓度也经常超过环境标准,这表明矿山土壤可能是长期的污染资源。从批式和柱式实验的结果来看,土壤性质(如土壤pH、氧化还原电位等)通过溶解度变化、络合物形成、沉淀等影响淋滤行为。二元动力学淋滤模型主要用于理解由于土壤的非均匀物理结构和不同结合位点导致的金属的非平衡淋滤行为。同时,降雨模式引起的水动力特性变化可以通过改变土壤与水之间的界面相互作用来影响淋滤行为。一些研究人员认识到,气候变化导致的极端天气条件(高温和干旱期增加)可以提高重金属的初始浸出浓度。在现实中,气候变化可通过影响土壤系统的水动力条件和化学稳定性导致金属的非平衡淋滤。因此,未来有必要对废弃矿区的重金属浸出行为进行精确预测,并设计废弃矿区应对气候变化的风险管理。
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引用次数: 0
차세대 항생제에 내성을 나타내는 식품 유래 슈퍼박테리아의 출현과 분자역학적 특성 显示对新一代抗生素耐药性的食物来源超级细菌的出现和分子力学特性
Pub Date : 2022-12-31 DOI: 10.33147/lsnrr.2022.30.1.1
Sun-Hee Kim, Jong-Hyun Yoo, Jae-Soon Oh, Hataek Kwon, Young-Min Chi, Min-Hyeok Cha
Superbacteria are antimicrobial resistant bacteria, and their emergence and widespread became great threats to humanity. Next-generation antimicrobials were developed to deal with existing antimicrobial resistance, but resistant bacteria emerged and spread immediately due to indiscriminate use of antimicrobials. In particular, the foodborne superbacteria are highly serious problem because they are closely related to the overall human life. In this article, we explored the distribution and resistance level of foodborne colistin-resistant Salmonella and linezolid-resistant Enterococcus among through the latest research papers about their resistance and molecular epidemiological transmission route. The transmission of resistance factors between food-derived superbacteria and other environment was also confirmed for the case of next-generation antimicrobials. Therefore, it is increasingly important to develop national and global resistance monitoring projects with One-health scheme.
超级细菌是对抗菌素具有耐药性的细菌,它们的出现和广泛传播对人类构成了巨大威胁。开发新一代抗菌素是为了应对现有的抗菌素耐药性,但由于滥用抗菌素,耐药细菌立即出现并传播。特别是,食源性超级细菌是一个非常严重的问题,因为它们与人类的整体生活密切相关。本文通过对食源性耐粘菌素沙门氏菌和耐利奈唑胺肠球菌耐药性及分子流行病学传播途径的最新研究,探讨了两种细菌的分布及耐药水平。新一代抗菌剂的耐药因子在食物来源的超级细菌和其他环境之间的传播也得到了证实。因此,利用“一种健康计划”制定国家和全球耐药性监测项目变得越来越重要。
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引用次数: 0
인간 허피스바이러스에 대한 G-quadruplex 결합 리간드의 항바이러스 효과 G-quadruplex结合配体对人类hupeis病毒的抗病毒效果
Pub Date : 2022-12-31 DOI: 10.33147/lsnrr.2022.30.1.23
Ji Ho Han, Moon Jung Song
G-quadruplexes (G4s) are noncanonical secondary nucleic acid structures constituted by stacking of guanine rich planar shaped tetrad formations that form a complex. G4s are implicated for various important roles in key cellular processes transcription, translation, telomere maintenance, epigenetic regulation, replication, and recombination. G-quadruplexes were first discovered as important structures in oncology, but for the past decade its relevance in viruses is becoming more evident. Human herpesviruses are DNA viruses of the Herpesviridae family and are unique in characteristic with two types of infection which can be distinguished by lytic and latency establishment in the host. During latency the virus maintains lifelong dormancy and intermittently undergoes reactivation, causing the host medical problems. Recently there are increasing number of reports regarding role of G4s in viral genomes and the potential antiviral efficacy of G4 ligands, including G4s in latency. Many results suggest viral G4s play significant roles in the virus life cycle and treatment of G4 ligands exhibit antiviral activities in both lytic and latent infections. In this review, the importance of G4s in herpesvirus genomes will be introduced with the potent G4 ligands used to study these mechanisms and finally explain the distinct functional properties of each G4 ligands.
g -四聚体(G4s)是由富含鸟嘌呤的平面四聚体叠合而成的非典型二级核酸结构。G4s在转录、翻译、端粒维持、表观遗传调控、复制和重组等关键细胞过程中发挥着重要作用。g -四联体最初是作为肿瘤中的重要结构被发现的,但在过去的十年中,它在病毒中的相关性变得越来越明显。人类疱疹病毒是疱疹病毒科的DNA病毒,具有独特的特征,有两种感染类型,可以通过在宿主体内的溶解性和潜伏期建立来区分。在潜伏期,病毒保持终身休眠,并间歇性地经历重新激活,导致宿主出现医疗问题。近年来,关于G4s在病毒基因组中的作用以及G4配体(包括潜伏期中的G4s)的潜在抗病毒功效的报道越来越多。许多研究结果表明,病毒G4s在病毒生命周期中发挥着重要作用,G4配体的治疗在裂解性和潜伏性感染中都表现出抗病毒活性。本文将介绍G4s在疱疹病毒基因组中的重要性,以及用于研究这些机制的有效G4配体,并最终解释每种G4配体的不同功能特性。
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引用次数: 0
인간 ATP 결합카세트 수퍼페미리 단백질 분류를 위한 서브페미리 특이적인 모티프 프로화일링 用于人类ATP结合卡式超肺美里蛋白质分类的亚肺美里特殊主题proceleing
Pub Date : 2022-12-31 DOI: 10.33147/lsnrr.2022.30.1.41
Gregory E. Sims, In-Geol Choi
The ATP binding cassette (ABC) transporter superfamily is ubiquitous in all three domains of life. The cellular and molecular functions of ABC transporters are diverse and essential to all living organisms. In human, mutations associated with ABC transporters are known to cause many genetic diseases. Although the members of ABC transporter superfamily share common sequential feature, the subfamily recognition is not a trivial task because of its domain complexity. The functional annotation begins with subfamily recognition. Here, we showed that a motif profiling provides simple and intuitive approach to recognize the subfamily level. Along with relation to the structural feature, the motif profiling approach will be a guide to annotate and understand molecular functions of subfamilies with a big superfamily
ATP结合盒转运蛋白超家族在生命的所有三个领域中都是普遍存在的。ABC转运体的细胞和分子功能是多种多样的,对所有生物体都是必不可少的。在人类中,已知与ABC转运蛋白相关的突变可引起许多遗传疾病。尽管ABC转运蛋白超家族的成员具有共同的序列特征,但由于其域的复杂性,子家族识别并不是一项简单的任务。功能注释从子族识别开始。在这里,我们表明,基序分析提供了一个简单和直观的方法来识别亚族水平。与结构特征的关系,基序分析方法将为注释和理解具有大超家族的亚家族的分子功能提供指导
{"title":"인간 ATP 결합카세트 수퍼페미리 단백질 분류를 위한 서브페미리 특이적인 모티프 프로화일링","authors":"Gregory E. Sims, In-Geol Choi","doi":"10.33147/lsnrr.2022.30.1.41","DOIUrl":"https://doi.org/10.33147/lsnrr.2022.30.1.41","url":null,"abstract":"The ATP binding cassette (ABC) transporter superfamily is ubiquitous in all three domains of life. The cellular and molecular functions of ABC transporters are diverse and essential to all living organisms. In human, mutations associated with ABC transporters are known to cause many genetic diseases. Although the members of ABC transporter superfamily share common sequential feature, the subfamily recognition is not a trivial task because of its domain complexity. The functional annotation begins with subfamily recognition. Here, we showed that a motif profiling provides simple and intuitive approach to recognize the subfamily level. Along with relation to the structural feature, the motif profiling approach will be a guide to annotate and understand molecular functions of subfamilies with a big superfamily","PeriodicalId":316688,"journal":{"name":"Institute of Life Science and Natural Resources","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134454230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Linking the Korean Emission Trading Scheme with the EU Emission Trading Scheme: What to Consider and How 连接韩国排放交易计划与欧盟排放交易计划:考虑什么和如何考虑
Pub Date : 2022-12-31 DOI: 10.33147/lsnrr.2022.30.1.101
Kun A Kim, S. Yang
The world has put various efforts to reduce greenhouse gas emissions to respond to the threat of climate change. Recently, the EU announced to introduce the 'Carbon Border Adjustment Mechanism (CBAM)' which imposes a sort of tariff to the import products from the countries which do not impose the proper level of carbon reduction cost. Korea, as a major exporter to the EC, should respond to the CBAM to reduce the damage. Linking the Korean Emission Trading Scheme with the EU counterpart would be one way. For such a linkage, it is necessary to consider not only technical, administrative, and economic conditions, but also political and popular factors. This study seek several considerations and a proper method for the linkage.
为了应对气候变化的威胁,世界各国在减少温室气体排放方面做出了各种努力。最近,欧盟宣布引入“碳边界调整机制”(CBAM),即对来自未征收适当碳减排成本的国家的进口产品征收某种关税。韩国作为欧盟的主要出口国,应该积极应对CBAM,减少损失。将韩国的排放交易计划与欧盟的相应计划联系起来将是一种方法。要实现这种联动,不仅要考虑技术、行政和经济条件,还要考虑政治和民众因素。本文探讨了联动的几点考虑和合适的方法。
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引用次数: 0
Investigation of automated nutrient solution feeding variables based on the slab weight for efficient water and nutrient management of paprika production 基于板重的自动化营养液进料变量对辣椒生产中水分和养分有效管理的研究
Pub Date : 2022-12-31 DOI: 10.33147/lsnrr.2022.30.1.67
Jongyei Kim, J. Baek, Jongyun Kim
This study was conducted to investigate potential variables that can be used in slab weight-based automated nutrient solution feeding algorithms for efficient water and nutrient management for paprika production in Korea. A slab weight measurement system was installed at paprika production farms in two provinces (Gangwon and Gyeongsangnam-do) in Korea, and we analyzed the fertigation data at production farms, and to investigate the variables applicable to the weight control-based automated nutrient feeding system. As the result, the difference in the amount of fertigation was mostly dependent on the growing season rather than the region, and the daily fertigation amount was determined by the number of fertigation applications per day, suggesting environmental data such as temperature and relative humidity would be the required variable for season estimation, and the number of fertigation applications would be the control variable for automated fertigation system. The sunrise and sunset times were the most critical variables for fertigation timing, suggesting the weather information will need to be integrated into the system. The demand for fertigation was different depending on the crop growth stages, thus numerical variables that can quantify the crop growth stages should be developed further. Further study for the leaching fraction would be required to use it for the potential control variable, since complex considerations for the electrical conductivity of the nutrient solution supplied and drained and the fertigation amount per application would affect the leaching fraction value. Consequently, the crop weight change data could provide the information for analysis of nutrient feeding in the production farms, and we could consider the various measurement and control variables that can be used in the weight-based automated nutrient feeding system.
本研究旨在研究可用于基于板重的自动营养液饲喂算法的潜在变量,以实现韩国辣椒生产中有效的水和养分管理。在韩国两个道(江原和庆尚南道)的辣椒生产农场安装了板坯重量测量系统,分析了生产农场的施肥数据,并调查了适用于基于体重控制的自动化营养喂养系统的变量。结果表明,施肥量的差异主要取决于生长季节而非区域,日施肥量由日施肥次数决定,表明温度、相对湿度等环境数据可作为季节估算的必要变量,施肥次数可作为自动化施肥系统的控制变量。日出和日落时间是施肥时间最关键的变量,这表明需要将天气信息整合到系统中。作物生育期对施肥的需求不同,因此需要进一步开发量化作物生育期的数值变量。为了将其作为潜在控制变量,需要对浸出分数进行进一步的研究,因为对供排水营养液的电导率和每次施用的施肥量的复杂考虑会影响浸出分数值。因此,作物体重变化数据可以为生产农场的营养喂养分析提供信息,我们可以考虑在基于体重的自动化营养喂养系统中使用各种测量和控制变量。
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引用次数: 0
Response to Salinity Stress in Plants and nitrogen effect: About hormone signaling pathway and root development 植物对盐胁迫的响应及氮素效应:关于激素信号通路与根系发育
Pub Date : 2022-12-31 DOI: 10.33147/lsnrr.2022.30.1.15
Won Je Le, Hojoung Lee
Around the world, 110 billion hectares of cultivated land in more than 100 countries are affected by soil salinity due to rapid climate change. Salinity and drought stress among various abiotic stresses are the main causes of significant decrease in crop production worldwide. In the 21st century, there are also predictions that 50% of the arable land will be affected by soil salinity. To alleviate the salt stress of crops and further increase production, mechanisms related to various physiological phenomena related to plant salt stress should be identified at the molecular level. In addition, plants can not move, so in order to successfully grow and develop in a given environment, various nutrients including micronutrients such as iron, manganese, and zinc are needed as well as large nutrients such as nitrogen, phosphorus and potassium. Most of these soil nutrients are absorbed into the plant body through the underground root system of a wide range of plants. In particular, nitrogen is one of the essential macronutrients for plant growth and crop productivity. Plants evolved various mechanisms to adapt to unbalanced nitrogen conditions. However, plants absorb nitrates with the help of nitrogen-fixing-related bacteria around their roots, as they cannot absorb nitrogen directly from the atmosphere. To meet the growing demand for food resources, the large amount of synthetic nitrogen fertilizer supplied to crops increased dramatically as synthetic nitrogen fertilizers began to be developed, resulting in a significant increase in crop yields. However, most of the nitrates deposited in the soil are not absorbed by plants but spread to the surrounding environment, leading to serious environmental and ecological pollution. Thus, identification of regulatory genes associated with in-depth understanding of nitrate absorption, assimilation and their use mechanisms is a key prerequisite for improving nitrogen use efficiency (NUE) in crops, and consequently is critical for maintaining agricultural stability.
由于气候迅速变化,全球100多个国家的1100亿公顷耕地受到土壤盐碱化的影响。在各种非生物胁迫中,盐胁迫和干旱胁迫是全球作物产量显著下降的主要原因。在21世纪,也有人预测,50%的耕地将受到土壤盐碱化的影响。为了缓解作物的盐胁迫,进一步提高产量,需要从分子水平上认识植物盐胁迫相关的各种生理现象的相关机制。此外,植物不能移动,因此为了在特定环境中成功生长和发育,需要各种营养物质,包括铁、锰和锌等微量营养物质以及氮、磷和钾等大量营养物质。这些土壤养分大部分通过各种植物的地下根系被植物体吸收。特别是氮是植物生长和作物生产所必需的大量营养元素之一。植物进化出各种机制来适应不平衡的氮条件。然而,由于植物不能直接从大气中吸收氮,因此植物吸收硝酸盐需要根部周围与固氮有关的细菌的帮助。为了满足日益增长的粮食资源需求,随着合成氮肥的开发,大量的合成氮肥供应给作物,导致作物产量显著提高。然而,沉积在土壤中的硝酸盐大部分没有被植物吸收,而是扩散到周围环境,造成了严重的环境和生态污染。因此,鉴定与深入了解硝酸盐吸收、同化及其利用机制相关的调控基因是提高作物氮素利用效率(NUE)的关键前提,对维持农业稳定至关重要。
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引用次数: 0
Impacts of the Fukushima Nuclear Disaster on the Domestic Seafood Price: An Event Study on Food Safety 福岛核灾难对国内海产品价格的影响:食品安全事件研究
Pub Date : 2022-12-31 DOI: 10.33147/lsnrr.2022.30.1.115
Hongkwon Yoo, Won-jun Chung, Hanyong Shim, Byeongseon Seo
The principal objective of this study is to provide an assessment on the effect of the contaminated water leakage at the Fukushima nuclear power plant on the price of domestic seafood such as pollock and mackerel using the event study. For the analysis, we design an event study by constructing the period before the leakage of contaminated water as the estimation window, and the period from the time of the occurrence of contaminated water leakage as the evaluation window. Based on the long-run equilibrium relationship between domestic seafood price and import price, our study investigates that the price of domestic seafood deviates from the equilibrium relationship with the import price after the leak of contaminated water. The empirical results demonstrate evidence that domestic seafood prices deviates from the equilibrium relationship in the evaluation window after the leakage of contaminated water. Consequently the price deviation indicates abnormal movement, which was found persistent and significant.
本研究的主要目的是利用事件研究评估福岛核电站污染水泄漏对鳕鱼和鲭鱼等国内海产品价格的影响。为了进行分析,我们设计了一个事件研究,将污染水泄漏前的时间段作为估计窗口,将污染水泄漏发生后的时间段作为评价窗口。本文基于国内海产品价格与进口价格的长期均衡关系,考察污染水泄漏后,国内海产品价格偏离了与进口价格的均衡关系。实证结果表明,污染水泄漏后,国内海产品价格偏离了评价窗口内的均衡关系。因此,价格偏离表明异常运动,这是发现持续和显著。
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引用次数: 0
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