Pub Date : 2021-12-28DOI: 10.29238/teknolabjournal.v10i2.310
Fitri Nuroini, K. Ratnaningrum, Mochamad Restinu Alya Soejoto, S. S. Dewi, G. S. A. Putri
Infection by Staphylococcus aureus, which contains alpha hemolysis and toxins that induce skin necrosis, can occur if incisions are left untreated. Infectious wound treatment that isn't dosed properly might have negative effects including bacterial resistance, hence plant-based solutions like binahong (Anredera cordifolia (Ten.) Steenis) leaf extract is needed. Alkaloids, saponins, and flavonoids found in binahong leaf act as anti-inflammatory, antiseptic, increase fibroblast cells, and increase collagen production throughout the healing process and scar tissue creation. Collagen production begins on day 3 of the proliferative stage and continues to increase until day 21. The goal of the study was to determine the thickness of collagen tissue using Masson's Trichrome staining on the 8th day of wound healing infected with S. aureus after administering binahong leaf extract. The research method used a completely randomized design with 4 research groups, normal control, negative control, treatment 25% (P1), and 50% (P2) concentrations of binahong leaf extract. S. aureus was cultured using BHI, MC, and BHIA media (Mc Farlan standard 0.5; 20L). Wounds were made on the back skin of the rat with a 2cm of length and 2mm of depth, then induced by S. aureus. The Binahong leaf extract was applied to the wound area in the morning and evening for 8th days. The paraffin method was used to prepare slides of the skin tissue, 10% NBF as fixation solution, and stained with Masson’s Trichrome. The thickness was measured in 5 fields of view at 400x magnification with a score of 0-4. The results showed that the P2 group had the same average collagen thickness as the normal control group, which was 50% in each field of view (score 3). While the average thickness of the P1 group was 25% in each field of view (score 2). These results indicate that the administration of binahong leaf extract at 25% and 50% concentrations can stimulate the formation of collagen on S. aureus-infected wounds.
{"title":"Binahong leaf extract activity in the 8th day of wound healing infected with Staphylococcus aureus towards collagen tissue","authors":"Fitri Nuroini, K. Ratnaningrum, Mochamad Restinu Alya Soejoto, S. S. Dewi, G. S. A. Putri","doi":"10.29238/teknolabjournal.v10i2.310","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v10i2.310","url":null,"abstract":"Infection by Staphylococcus aureus, which contains alpha hemolysis and toxins that induce skin necrosis, can occur if incisions are left untreated. Infectious wound treatment that isn't dosed properly might have negative effects including bacterial resistance, hence plant-based solutions like binahong (Anredera cordifolia (Ten.) Steenis) leaf extract is needed. Alkaloids, saponins, and flavonoids found in binahong leaf act as anti-inflammatory, antiseptic, increase fibroblast cells, and increase collagen production throughout the healing process and scar tissue creation. Collagen production begins on day 3 of the proliferative stage and continues to increase until day 21. The goal of the study was to determine the thickness of collagen tissue using Masson's Trichrome staining on the 8th day of wound healing infected with S. aureus after administering binahong leaf extract. The research method used a completely randomized design with 4 research groups, normal control, negative control, treatment 25% (P1), and 50% (P2) concentrations of binahong leaf extract. S. aureus was cultured using BHI, MC, and BHIA media (Mc Farlan standard 0.5; 20L). Wounds were made on the back skin of the rat with a 2cm of length and 2mm of depth, then induced by S. aureus. The Binahong leaf extract was applied to the wound area in the morning and evening for 8th days. The paraffin method was used to prepare slides of the skin tissue, 10% NBF as fixation solution, and stained with Masson’s Trichrome. The thickness was measured in 5 fields of view at 400x magnification with a score of 0-4. The results showed that the P2 group had the same average collagen thickness as the normal control group, which was 50% in each field of view (score 3). While the average thickness of the P1 group was 25% in each field of view (score 2). These results indicate that the administration of binahong leaf extract at 25% and 50% concentrations can stimulate the formation of collagen on S. aureus-infected wounds.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44644007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-26DOI: 10.29238/teknolabjournal.v10i2.315
Hedya Nadhrati Surura, H. Abdullah, Sahna Ferdinand Ginting, Erny Tandanu, Refi Ikhtiari
This research aims to develop a silver nanoparticle (AgNPs) using several plant extracts for antibacterial application. The papaya seed extract has been fractioned by n-hexane, ethyl acetate, water, and ethanol. The n-hexane fraction was the only fraction that succeeded in synthesizing AgNPs. The characterization methods showed AgNPs marked at 430 nm with UV-Vis and 1640 cm-1 with FTIR. SEM observed the aggregation of spherical AgNPs at the 200 nm scale. The particle size of 91.3 nm was measured with PSA that confirmed the nanoscale of the synthesized material. All fractions contained alkaloid compound, and ethyl acetate fraction showed a group of indole with specific wavenumber at 2623 cm-1, 1737 cm-1, and 1237 cm-1 representing N-H, C=O, C-N, respectively. All fractions at every concentration (25%, 50%, 75%, 100%) have been tested and showed the medium effect on bacterial growth inhibition. Among all fractions, the AgNPs n-hexane fraction has the highest bacterial effect, which was indicated by mean values of inhibition zone 7.2 mm against S.aureus, as well as 6.6 mm against E.coli. ANOVA analysis showed that AgNPs n-hexane fraction has a significant inhibition zone compared to other fractions against S.aureus (p=0.002), but not significant to E.coli (p=0.128). The insignificant results on E.coli because of gram-negative bacteria's biophysical characteristics, such as membrane cell wall and flagellin. This research emphasized that AgNPs could be synthesized via a green process of nucleation by using plant extract that effectively inhibits the growth of S.aureus and E.coli. Further studies on the mechanism of the antibacterial effect at the molecular level might be investigated soon.
{"title":"Green synthesis of silver nanoparticles from papaya seed extracts with alkaloid content for antibacterial application","authors":"Hedya Nadhrati Surura, H. Abdullah, Sahna Ferdinand Ginting, Erny Tandanu, Refi Ikhtiari","doi":"10.29238/teknolabjournal.v10i2.315","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v10i2.315","url":null,"abstract":"This research aims to develop a silver nanoparticle (AgNPs) using several plant extracts for antibacterial application. The papaya seed extract has been fractioned by n-hexane, ethyl acetate, water, and ethanol. The n-hexane fraction was the only fraction that succeeded in synthesizing AgNPs. The characterization methods showed AgNPs marked at 430 nm with UV-Vis and 1640 cm-1 with FTIR. SEM observed the aggregation of spherical AgNPs at the 200 nm scale. The particle size of 91.3 nm was measured with PSA that confirmed the nanoscale of the synthesized material. All fractions contained alkaloid compound, and ethyl acetate fraction showed a group of indole with specific wavenumber at 2623 cm-1, 1737 cm-1, and 1237 cm-1 representing N-H, C=O, C-N, respectively. All fractions at every concentration (25%, 50%, 75%, 100%) have been tested and showed the medium effect on bacterial growth inhibition. Among all fractions, the AgNPs n-hexane fraction has the highest bacterial effect, which was indicated by mean values of inhibition zone 7.2 mm against S.aureus, as well as 6.6 mm against E.coli. ANOVA analysis showed that AgNPs n-hexane fraction has a significant inhibition zone compared to other fractions against S.aureus (p=0.002), but not significant to E.coli (p=0.128). The insignificant results on E.coli because of gram-negative bacteria's biophysical characteristics, such as membrane cell wall and flagellin. This research emphasized that AgNPs could be synthesized via a green process of nucleation by using plant extract that effectively inhibits the growth of S.aureus and E.coli. Further studies on the mechanism of the antibacterial effect at the molecular level might be investigated soon.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45103892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-26DOI: 10.29238/teknolabjournal.v10i2.291
Axel Fortunio Shiloputra, A. A. Parikesit, J. T. Darmawan, Viona Pricillia, D. D. R. Turista, A. Ansori
The recent outbreak of SARS-CoV-2 across the globe and the absence of a specific cure against the disease lead the scientific community to investigate some alternative indigenous treatments. SARS-CoV-2 is the virus responsible for the coronavirus ailment 2019 (COVID-19). This virus has 4 auxiliary proteins namely the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The main proteases and RNA dependent RNA polymerase are also essential structures by which the virus replicates and survives. Each of these proteins are structures of the virus that are potential targets for drugs which are leads in the drug discovery process of any drug for the virus. Currently available treatments are not specific to the disease and therefore carry unwanted adverse effects that can be highly dangerous and sometimes fatal. Many of these treatments are supplementary in nature or based on repurposed drugs from other viral outbreaks. Alternatives of conventional drugs are required to control the spread and severity of the disease. Allicin, curcumin and their derivatives have been researched for their antiviral property and shown to have good binding affinity towards SARS-CoV-2 structures essential in their survival, especially the main proteases and RNA dependent RNA polymerases. The structural bioinformatics tools have elicited methods to predict the bioactivity of the natural product-based compounds. Apart from the beneficial medication that they offer, natural products carry along other advantages for the current pandemic situation in terms of supply, logistics, and affordability.
{"title":"An Overview of the Curcumin-Based and Allicin Bioactive Compounds as potential treatment to SARS-CoV-2 with structural bioinformatics tools","authors":"Axel Fortunio Shiloputra, A. A. Parikesit, J. T. Darmawan, Viona Pricillia, D. D. R. Turista, A. Ansori","doi":"10.29238/teknolabjournal.v10i2.291","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v10i2.291","url":null,"abstract":"The recent outbreak of SARS-CoV-2 across the globe and the absence of a specific cure against the disease lead the scientific community to investigate some alternative indigenous treatments. SARS-CoV-2 is the virus responsible for the coronavirus ailment 2019 (COVID-19). This virus has 4 auxiliary proteins namely the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The main proteases and RNA dependent RNA polymerase are also essential structures by which the virus replicates and survives. Each of these proteins are structures of the virus that are potential targets for drugs which are leads in the drug discovery process of any drug for the virus. Currently available treatments are not specific to the disease and therefore carry unwanted adverse effects that can be highly dangerous and sometimes fatal. Many of these treatments are supplementary in nature or based on repurposed drugs from other viral outbreaks. Alternatives of conventional drugs are required to control the spread and severity of the disease. Allicin, curcumin and their derivatives have been researched for their antiviral property and shown to have good binding affinity towards SARS-CoV-2 structures essential in their survival, especially the main proteases and RNA dependent RNA polymerases. The structural bioinformatics tools have elicited methods to predict the bioactivity of the natural product-based compounds. Apart from the beneficial medication that they offer, natural products carry along other advantages for the current pandemic situation in terms of supply, logistics, and affordability.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43052238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-21DOI: 10.29238/teknolabjournal.v10i1.238
Lina Rahmawati Rizkuloh, A. Pratita, Mediana Mediana, A. Yuliana
Toxicity prediction is very important for the development and design of new drugs because computational toxicity estimates are not only faster than the determination of toxic doses in animals, but can also help reduce the number of animal trials. The test uses pkCSM, Protox II, Toxtree, preADMET and T.E.S.T. From the results of research that has been carried out on 57 pigment derivated compounds of Monascus sp. mold, the results of the pkCSM application are 39 test compounds for the Protox-II application there is 1 compound, the Toxtree application produces 1 compound, for the PreADMET application 4 safe compounds are produced, and for the T.E.S.T application produces 1 compound because it fulfills one of the aspects of ICH (International Conference on Harmonization) S9: non-clinical evaluation for anticancer pharmaceutical 2010 and has the potential as a candidate for anticancer drugs. .
毒性预测对于新药的开发和设计非常重要，因为计算毒性估计不仅比确定动物毒性剂量更快，而且还有助于减少动物试验的次数。测试使用pkCSM, Protox II, Toxtree, preADMET和T.E.S.T.。从对红曲霉霉菌的57种色素衍生化合物进行的研究结果来看，pkCSM应用的结果是39种测试化合物，Protox-II应用产生1种化合物，Toxtree应用产生1种化合物，preADMET应用产生4种安全化合物。并为T.E.S.T申请生产1种化合物，因为它满足ICH(国际协调会议)S9的一个方面:2010年抗癌药物的非临床评价，并有潜力作为抗癌药物的候选药物。
{"title":"In silico study in toxicity parameters of Pigment Derivated Compounds of Monascus sp. mold as a cervical anti-cancer drugs candidate","authors":"Lina Rahmawati Rizkuloh, A. Pratita, Mediana Mediana, A. Yuliana","doi":"10.29238/teknolabjournal.v10i1.238","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v10i1.238","url":null,"abstract":"Toxicity prediction is very important for the development and design of new drugs because computational toxicity estimates are not only faster than the determination of toxic doses in animals, but can also help reduce the number of animal trials. The test uses pkCSM, Protox II, Toxtree, preADMET and T.E.S.T. From the results of research that has been carried out on 57 pigment derivated compounds of Monascus sp. mold, the results of the pkCSM application are 39 test compounds for the Protox-II application there is 1 compound, the Toxtree application produces 1 compound, for the PreADMET application 4 safe compounds are produced, and for the T.E.S.T application produces 1 compound because it fulfills one of the aspects of ICH (International Conference on Harmonization) S9: non-clinical evaluation for anticancer pharmaceutical 2010 and has the potential as a candidate for anticancer drugs. \u0000.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44404982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-05DOI: 10.29238/teknolabjournal.v10i1.285
Linda Augustien Makalew, Elne Vieke Rambi, Muhammad Ali Makaminan, Risman S Duka, T. Hiola
The Covid-19 pandemic that has caught the attention of not only Indonesia but the rest of the world. The 3M movement, namely using masks, washing hands and keeping a distance to minimize the transmission of tuberculosis, is very useful for the eradication of TB tuberculosis. However, this is a challenge in itself for microscopic officers at the Microscopic Reference Center (MRC) in education and handling of sputum for tuberculosis patients who are currently being treated. The purpose of this study was to test VisKomLAM 1, 2, 3 and 4 in bridging communication between officers in sputum management education for tuberculosis sufferers at each examination during the Covid-19 pandemic. The research locations took MRC Tuminting, Wawonasa and Tikala Baru in Manado City and MRC Telaga in Gorontalo District. Analysis with paired t test showed a significance below 5%, which means that VisKomLAM 1, 2, 3 and 4 can bridge education from officers to tuberculosis sufferers in the Covid-19 pandemic era. It is recommended that health workers always strive to promote tuberculosis health in achieving tuberculosis elimination by 2050.
{"title":"Digital communication in health promotion in handling tuberculosis sputum","authors":"Linda Augustien Makalew, Elne Vieke Rambi, Muhammad Ali Makaminan, Risman S Duka, T. Hiola","doi":"10.29238/teknolabjournal.v10i1.285","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v10i1.285","url":null,"abstract":"The Covid-19 pandemic that has caught the attention of not only Indonesia but the rest of the world. The 3M movement, namely using masks, washing hands and keeping a distance to minimize the transmission of tuberculosis, is very useful for the eradication of TB tuberculosis. However, this is a challenge in itself for microscopic officers at the Microscopic Reference Center (MRC) in education and handling of sputum for tuberculosis patients who are currently being treated. The purpose of this study was to test VisKomLAM 1, 2, 3 and 4 in bridging communication between officers in sputum management education for tuberculosis sufferers at each examination during the Covid-19 pandemic. The research locations took MRC Tuminting, Wawonasa and Tikala Baru in Manado City and MRC Telaga in Gorontalo District. Analysis with paired t test showed a significance below 5%, which means that VisKomLAM 1, 2, 3 and 4 can bridge education from officers to tuberculosis sufferers in the Covid-19 pandemic era. It is recommended that health workers always strive to promote tuberculosis health in achieving tuberculosis elimination by 2050.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42683777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-05DOI: 10.29238/teknolabjournal.v10i1.286
Elne Vieke Rambi, D. Sukandar, Linda Augustien Makalew, Yohanis A. Tomastola, Ketrina Konoralma
Diabetes mellitus (DM) with pulmonary tuberculosis (TB) is an infectious disease if not educated regularly, there will be a high risk of drug resistance and even some anti-tuberculosis drugs. This study aims to identify anti-tuberculosis drug resistance in DM patients with TB in Manado City. The population in the study types 2 DM patients as amount 80 patients. Based on TCM/GenExpert examination from 47 respondents, there were 17 respondents positive multi drugs resistance rifampicin (RR). Sampling taking based on inclusion criteria, i.e., have had type DM for five years, had suffered TB MDR RR based on GenXpert examination as much as 17 respondents followed in the resistance test with Sputum TB culture and MGIT method. The result of the study showed that MDR DM-TB with MGIT method as followed is obtained from 17 samples, six samples (35.30%) resistance INH 0.4 mg and 1 sample (5.88%) MDR canamycin, and still sensitive INH 0.4 mg and camaycin is ten samples (58.82%). This study results could be used to program planning of prevention and controlling efforts TB-DM in this treatment obedience and regimen anti-tuberculosis medicine for MDR-TB patients.
{"title":"Multi drugs resistance to Diabetes Mellitus patients with tuberculosis in Manado City","authors":"Elne Vieke Rambi, D. Sukandar, Linda Augustien Makalew, Yohanis A. Tomastola, Ketrina Konoralma","doi":"10.29238/teknolabjournal.v10i1.286","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v10i1.286","url":null,"abstract":"Diabetes mellitus (DM) with pulmonary tuberculosis (TB) is an infectious disease if not educated regularly, there will be a high risk of drug resistance and even some anti-tuberculosis drugs. This study aims to identify anti-tuberculosis drug resistance in DM patients with TB in Manado City. The population in the study types 2 DM patients as amount 80 patients. Based on TCM/GenExpert examination from 47 respondents, there were 17 respondents positive multi drugs resistance rifampicin (RR). Sampling taking based on inclusion criteria, i.e., have had type DM for five years, had suffered TB MDR RR based on GenXpert examination as much as 17 respondents followed in the resistance test with Sputum TB culture and MGIT method. The result of the study showed that MDR DM-TB with MGIT method as followed is obtained from 17 samples, six samples (35.30%) resistance INH 0.4 mg and 1 sample (5.88%) MDR canamycin, and still sensitive INH 0.4 mg and camaycin is ten samples (58.82%). This study results could be used to program planning of prevention and controlling efforts TB-DM in this treatment obedience and regimen anti-tuberculosis medicine for MDR-TB patients.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42427865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-05DOI: 10.29238/teknolabjournal.v10i1.279
Fitri Nuroini
Kualitas pewarnaan Diff-Quick bergantung pada beberapa faktor diantaranya adalah metode fiksasi yaitu fiksasi kering dan fiksasi basah. Kedua metode tersebut memiliki kekurangannya masing-masing, fiksasi kering dapat menyebabkan pecahnya sel sedangkan fiksasi basah lebih memakan waktu dan biaya dalam pengerjaannya, namun fiksasi kering lebih sering digunakan karena lebih cepat dan murah. Tujuan penelitian untuk membandingkan hasil pewarnaan Diff-Quick sediaan sitologi yang difiksasi dengan metode fiksasi kering dan metode fiksasi basah. Jenis penelitian adalah penelitian eksperimen. Sampel diperoleh dari RS K.R.M.T. Wongsonegoro Semarang berjumlah 36 sediaan yang dibagi menjadi tiga kelompok yaitu kelompok kontrol, kelompok fiksasi kering, dan kelompok fiksasi basah. Ketiga kelompok tersebut diwarnai dengan Diff-Quick. Pewarnaan sediaan fiksasi kering memberikan hasil 4 sediaan kurang baik, 5 sediaan baik, dan 3 sediaan sangat baik sedangkan pewarnaan sediaan fiksasi basah memberikan hasil 0 sediaan kurang baik, 8 sediaan baik, dan 4 sediaan sangat baik. Hal ini menunjukkan hasil pewarnaan sediaan yang difiksasi basah memiliki kualitas yang cenderung lebih baik dan lebih konsisten dibanding hasil pewarnaan sediaan fiksasi kering. Meskipun secara statistik melalui uji Post-Hoc menunjukkan tidak ada perbedaan, kualitas pewarnaan Diff-Quick sediaan sitologi yang difiksasi dengan metode fiksasi basah lebih baik dari pada fiksasi kering.
{"title":"Air-Dried and Wet Fixation on Fine Needle Aspiration Biopsy (FNAB) Specimen","authors":"Fitri Nuroini","doi":"10.29238/teknolabjournal.v10i1.279","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v10i1.279","url":null,"abstract":"Kualitas pewarnaan Diff-Quick bergantung pada beberapa faktor diantaranya adalah metode fiksasi yaitu fiksasi kering dan fiksasi basah. Kedua metode tersebut memiliki kekurangannya masing-masing, fiksasi kering dapat menyebabkan pecahnya sel sedangkan fiksasi basah lebih memakan waktu dan biaya dalam pengerjaannya, namun fiksasi kering lebih sering digunakan karena lebih cepat dan murah. Tujuan penelitian untuk membandingkan hasil pewarnaan Diff-Quick sediaan sitologi yang difiksasi dengan metode fiksasi kering dan metode fiksasi basah. Jenis penelitian adalah penelitian eksperimen. Sampel diperoleh dari RS K.R.M.T. Wongsonegoro Semarang berjumlah 36 sediaan yang dibagi menjadi tiga kelompok yaitu kelompok kontrol, kelompok fiksasi kering, dan kelompok fiksasi basah. Ketiga kelompok tersebut diwarnai dengan Diff-Quick. Pewarnaan sediaan fiksasi kering memberikan hasil 4 sediaan kurang baik, 5 sediaan baik, dan 3 sediaan sangat baik sedangkan pewarnaan sediaan fiksasi basah memberikan hasil 0 sediaan kurang baik, 8 sediaan baik, dan 4 sediaan sangat baik. Hal ini menunjukkan hasil pewarnaan sediaan yang difiksasi basah memiliki kualitas yang cenderung lebih baik dan lebih konsisten dibanding hasil pewarnaan sediaan fiksasi kering. Meskipun secara statistik melalui uji Post-Hoc menunjukkan tidak ada perbedaan, kualitas pewarnaan Diff-Quick sediaan sitologi yang difiksasi dengan metode fiksasi basah lebih baik dari pada fiksasi kering.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44191664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-03DOI: 10.29238/TEKNOLABJOURNAL.V10I1.261
G. Erdal, N. Isiksacan, R. Korkusuz, P. Kasapoğlu, Kadriye Kart Yasaroglu
Gender differences affect the frequency and course of many diseases. This study aimed to determine the gender distribution in HIV-infected patients and investigate the relationship between gender and immune response. The study included HIV-infected patients who followed up in our hospital in 2018. The patients were divided into HIV RNA negative patients (Group 1) and HIV RNA positive patients (Group 2). Patients with diseases that may affect the immune system and those using drugs that affect the immune system were excluded from the study. The evaluation was made of 549 patients, as 305 patients (45 females 14.75%) in Group 1 and 224 patients (23 females, 9.43%) in Group 2. When the CD4/CD8 ratio of male and female patients was compared in both groups, a lower rate was determined in females (0.71-0.58) than males (0.82-0.93). A negative correlation was determined between HIV RNA and the CD4/CD8 ratio in premenopausal females (p=0.045) and males (≤45 years p=0.0001). Clinical studies of HIV infection have demonstrated better initial viremia control in females with primary infection, faster disease progression, and stronger immune activation than males for the same level of viral replication.
{"title":"Does gender affect immune response in HIV patients?","authors":"G. Erdal, N. Isiksacan, R. Korkusuz, P. Kasapoğlu, Kadriye Kart Yasaroglu","doi":"10.29238/TEKNOLABJOURNAL.V10I1.261","DOIUrl":"https://doi.org/10.29238/TEKNOLABJOURNAL.V10I1.261","url":null,"abstract":"Gender differences affect the frequency and course of many diseases. This study aimed to determine the gender distribution in HIV-infected patients and investigate the relationship between gender and immune response. The study included HIV-infected patients who followed up in our hospital in 2018. The patients were divided into HIV RNA negative patients (Group 1) and HIV RNA positive patients (Group 2). Patients with diseases that may affect the immune system and those using drugs that affect the immune system were excluded from the study. The evaluation was made of 549 patients, as 305 patients (45 females 14.75%) in Group 1 and 224 patients (23 females, 9.43%) in Group 2. When the CD4/CD8 ratio of male and female patients was compared in both groups, a lower rate was determined in females (0.71-0.58) than males (0.82-0.93). A negative correlation was determined between HIV RNA and the CD4/CD8 ratio in premenopausal females (p=0.045) and males (≤45 years p=0.0001). Clinical studies of HIV infection have demonstrated better initial viremia control in females with primary infection, faster disease progression, and stronger immune activation than males for the same level of viral replication.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43489231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-02DOI: 10.29238/teknolabjournal.v10i1.266
Osman Ozudogru, E. Yerlikaya, N. Alayunt, Z. Çambay
The purpose of this study is to investigate the healing effect of Favipiravir used in pre-intensive care treatment of patients diagnosed with COVID-19 in order to elucidate the pathogenesis and complications of coronavirus. The data regarding the clinical findings of the patients in the hospital information system and the biochemical parameters made standard in the treatment/follow-up of COVID 19 were taken from the system and evaluated retrospectively. In addition, it was examined as a whole with mild, moderate and severe pulmonary involvement compared to CT findings. Hemogram, coagulation and biochemistry parameters used in the diagnosis and follow-up of COVID-19 were evaluated. SPSS 22.0 statistics program for Windows was used in statistical analysis to evaluate the data obtained from patient files and hospital information system. There is no definitive treatment protocol within the scope of treatment. Drug studies are currently ongoing. In this study, the first clinical findings, treatment types and recovery times of patients diagnosed with COVID-19, the healing effect of favipiravir used before intensive care were determined. Between group 1 (those who started treatment within 0-5 days) and group 2 (those who started treatment within 6-10 days), after 5 days of favipravir treatment, when serum parameters were compared, favipravir treatment was statistically significantly lower in the first group that was started early, WBC, Neutrophil, Creatine, CK, CRP, D-Dimer, PCT, LDH. By collecting the data obtained as a result of the research, early deaths can be prevented worldwide. Our study recommending alternative treatment approaches is important for the protection of patients' quality of life. In this study, when all biochemical markers were evaluated together, it was evaluated that starting Favipiravir treatment early was beneficial in treating COVID-19 disease.
{"title":"Evaluation of favipiravir treatment before intensive care in COVID-19 patients","authors":"Osman Ozudogru, E. Yerlikaya, N. Alayunt, Z. Çambay","doi":"10.29238/teknolabjournal.v10i1.266","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v10i1.266","url":null,"abstract":"The purpose of this study is to investigate the healing effect of Favipiravir used in pre-intensive care treatment of patients diagnosed with COVID-19 in order to elucidate the pathogenesis and complications of coronavirus. The data regarding the clinical findings of the patients in the hospital information system and the biochemical parameters made standard in the treatment/follow-up of COVID 19 were taken from the system and evaluated retrospectively. In addition, it was examined as a whole with mild, moderate and severe pulmonary involvement compared to CT findings. Hemogram, coagulation and biochemistry parameters used in the diagnosis and follow-up of COVID-19 were evaluated. SPSS 22.0 statistics program for Windows was used in statistical analysis to evaluate the data obtained from patient files and hospital information system. There is no definitive treatment protocol within the scope of treatment. Drug studies are currently ongoing. In this study, the first clinical findings, treatment types and recovery times of patients diagnosed with COVID-19, the healing effect of favipiravir used before intensive care were determined. Between group 1 (those who started treatment within 0-5 days) and group 2 (those who started treatment within 6-10 days), after 5 days of favipravir treatment, when serum parameters were compared, favipravir treatment was statistically significantly lower in the first group that was started early, WBC, Neutrophil, Creatine, CK, CRP, D-Dimer, PCT, LDH. By collecting the data obtained as a result of the research, early deaths can be prevented worldwide. Our study recommending alternative treatment approaches is important for the protection of patients' quality of life. In this study, when all biochemical markers were evaluated together, it was evaluated that starting Favipiravir treatment early was beneficial in treating COVID-19 disease.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46251665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-02DOI: 10.29238/teknolabjournal.v10i1.276
A. Kartika, M. S. Fitria, Vanny Oktaviola
Caulerpa racemosa is a green algae consumed by people in northern coastal areas. C. racemosa has a habitat attached to the shallow seabed. C. racemosa usualy consumed fresh without any cooking process so that the contamination of microorganisms can be eaten. Molecular identification using 16S rRNA is needed to determine the type of bacterial contaminants in C. racemosa. The isolates of C. racemosa were cultured in HIA, BAP, and BHI media. Bacteria from BHI media were isolated by DNA, PCR for 16S rRNA gene, and sequencing. Bacteria isolate C. racemosa was found to have the α-hemolytic ability in BAP media. The sequencing analysis showed that the three bacterial colonies of C. racemosa isolate had high similarity with V. parahemolyticus, Caldalkalibacillus mannanilyticus, and Exiguobacterium profundum.
{"title":"Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa","authors":"A. Kartika, M. S. Fitria, Vanny Oktaviola","doi":"10.29238/teknolabjournal.v10i1.276","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v10i1.276","url":null,"abstract":"Caulerpa racemosa is a green algae consumed by people in northern coastal areas. C. racemosa has a habitat attached to the shallow seabed. C. racemosa usualy consumed fresh without any cooking process so that the contamination of microorganisms can be eaten. Molecular identification using 16S rRNA is needed to determine the type of bacterial contaminants in C. racemosa. The isolates of C. racemosa were cultured in HIA, BAP, and BHI media. Bacteria from BHI media were isolated by DNA, PCR for 16S rRNA gene, and sequencing. Bacteria isolate C. racemosa was found to have the α-hemolytic ability in BAP media. The sequencing analysis showed that the three bacterial colonies of C. racemosa isolate had high similarity with V. parahemolyticus, Caldalkalibacillus mannanilyticus, and Exiguobacterium profundum.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44168653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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