Pub Date : 2020-08-24DOI: 10.21203/rs.3.rs-57941/v1
Aifeng Liu, Jixin Chen, Shuwei Gong, Q. Wei, Ye Yuan
Background: The main role of the scaffold materials is to enable cells to survive in the scaffold binding as while as to further promote their proliferation and differentiation ability. For mesenchymal stem cell, the scaffold could provide an environment for them to maintain their phenotype, and synthesize all necessary molecules and proteins. Generally, scaffold materials for stem cell need to possess basic characteristics such as high porosity, large surface area, surface rigidity and biodeg-radability. Thus, the two-dimensional graphene oxide (GO) with oxygen-containing functional groups may be suitable scaffold materials for mesenchymal stem cell culture. In this study, the effect of GO on the value-added differentiation activity of mesenchymal stem cell was systematically investigated. Results: It was found that low concentration of GO and sufficient concentration of umbilical cord mesenchymal stem cells are suitable for the second Co-culture. Furthermore, the addition of hyaluronic acid will make this culture more evenly distributed. Conclusions: The adsorption of GO on umbilical cord mesenchymal stem cells can also make the two closely linked, which avoids the impact of animal joint activities on cells.
{"title":"Effects of Co-Culture of Graphene Oxide Scaffolds with Different Concentrations and Umbilical Mesenchymal Stem Cells on the Proliferation and Differentiation of Stem Cells","authors":"Aifeng Liu, Jixin Chen, Shuwei Gong, Q. Wei, Ye Yuan","doi":"10.21203/rs.3.rs-57941/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-57941/v1","url":null,"abstract":"\u0000 Background: The main role of the scaffold materials is to enable cells to survive in the scaffold binding as while as to further promote their proliferation and differentiation ability. For mesenchymal stem cell, the scaffold could provide an environment for them to maintain their phenotype, and synthesize all necessary molecules and proteins. Generally, scaffold materials for stem cell need to possess basic characteristics such as high porosity, large surface area, surface rigidity and biodeg-radability. Thus, the two-dimensional graphene oxide (GO) with oxygen-containing functional groups may be suitable scaffold materials for mesenchymal stem cell culture. In this study, the effect of GO on the value-added differentiation activity of mesenchymal stem cell was systematically investigated. Results: It was found that low concentration of GO and sufficient concentration of umbilical cord mesenchymal stem cells are suitable for the second Co-culture. Furthermore, the addition of hyaluronic acid will make this culture more evenly distributed. Conclusions: The adsorption of GO on umbilical cord mesenchymal stem cells can also make the two closely linked, which avoids the impact of animal joint activities on cells.","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123711029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-08-04DOI: 10.21203/rs.3.rs-52410/v1
Kehinde Sowunmi, Sofiyah Modupeola Adebayo, Ayobami Adesiyan Lawal, A. Kade, G. Kaur
We evaluated the toxicity of hepatic, hematological, and oxidative effects of binapacryl on female albino mice. The animals were treated orally with 50, 100, 150 and 200 mg/kg body weight of the binapacryl, on a daily basis for a period of 15 days. Distilled water was used as control treatment. Samples of blood and hepatic tissue were collected at the end of the treatment. Hepatotoxicity was monitored by quantitative analysis of the serum enzymes ALT, AST, γ-GT, urea and creatinine. We also investigated liver tissues histopathologically. Alterations of hematological parameters were monitored by RBC, WBC, hemoglobin, hematocrit, MCV, MCH, and MCHC. Significant increases in the levels of hepatic enzymes (ALT, AST, and γ-GT) were observed for both binapacryl treatments, but no considerable differences were found by histological analysis. The hematological parameters showed significant alterations (200mg/kg body weight) with reductions of RBC, hematocrit, and hemoglobin, together with a significant increase of MCV. There was an important increase in lipid peroxidation at both dosage levels, together with an NPSH decrease in the hepatic tissue, and significant changes in these parameters were observed only at the higher dose rate. The results of this study indicate that binapacryl can promote hematological and hepatic alterations, even at subacute exposure, which could be related to the induction of reactive oxygen species.
{"title":"Evaluation of Biochemical, Hematological and Oxidative Parameters in Mice exposed to the Binapacryl","authors":"Kehinde Sowunmi, Sofiyah Modupeola Adebayo, Ayobami Adesiyan Lawal, A. Kade, G. Kaur","doi":"10.21203/rs.3.rs-52410/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-52410/v1","url":null,"abstract":"\u0000 We evaluated the toxicity of hepatic, hematological, and oxidative effects of binapacryl on female albino mice. The animals were treated orally with 50, 100, 150 and 200 mg/kg body weight of the binapacryl, on a daily basis for a period of 15 days. Distilled water was used as control treatment. Samples of blood and hepatic tissue were collected at the end of the treatment. Hepatotoxicity was monitored by quantitative analysis of the serum enzymes ALT, AST, γ-GT, urea and creatinine. We also investigated liver tissues histopathologically. Alterations of hematological parameters were monitored by RBC, WBC, hemoglobin, hematocrit, MCV, MCH, and MCHC. Significant increases in the levels of hepatic enzymes (ALT, AST, and γ-GT) were observed for both binapacryl treatments, but no considerable differences were found by histological analysis. The hematological parameters showed significant alterations (200mg/kg body weight) with reductions of RBC, hematocrit, and hemoglobin, together with a significant increase of MCV. There was an important increase in lipid peroxidation at both dosage levels, together with an NPSH decrease in the hepatic tissue, and significant changes in these parameters were observed only at the higher dose rate. The results of this study indicate that binapacryl can promote hematological and hepatic alterations, even at subacute exposure, which could be related to the induction of reactive oxygen species.","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114458256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ghaffari, S. Rakhshanderou, M. Riahi, Y. Mehrabi, H. Soori, Javad Harooni
Background: No behavior as much as the use of a seatbelt affects the reduction of the severity of injuries caused by driving accidents. So far, there has not been sufficient evidence of predictive factors regarding the use of seatbelts in bus passengers. The purpose of this study was to determine the predictors of seatbelt use in passengers of intercity buses. Methods: In this cross-sectional study, 458 passengers of intercity bus terminals participated. The sampling method was multistage in this study. To collect data, a researcher-made questionnaire whose validity and reliability were confirmed was used. The collected data were analyzed using SPSS software version 22 and logistic regression test. Results: The study population included 47% of men and 53% of women with an average age of 28.35 ± 6.1. Police stations, high bus speeds, night, and inappropriate weather conditions were among the situations in which the use of a seatbelts was more on the bus. The results of the logistic regression test in the third model showed that perceived barriers, law and supervision, subjective norms, environment, and bus type predict the use of seat belts significantly (P <0.000). Conclusions: It seems that in addition to interventions designed to increase the knowledge and attitude of passengers and enhance the use of seatbelts on the bus, a special look is also needed at important people who influence the behavior of the people (subjective norms).
{"title":"Behavior and non-behavior Predictors of seat belt use amongst Iranian Bus Passengers","authors":"M. Ghaffari, S. Rakhshanderou, M. Riahi, Y. Mehrabi, H. Soori, Javad Harooni","doi":"10.21203/rs.2.20834/v1","DOIUrl":"https://doi.org/10.21203/rs.2.20834/v1","url":null,"abstract":"\u0000 Background: No behavior as much as the use of a seatbelt affects the reduction of the severity of injuries caused by driving accidents. So far, there has not been sufficient evidence of predictive factors regarding the use of seatbelts in bus passengers. The purpose of this study was to determine the predictors of seatbelt use in passengers of intercity buses. Methods: In this cross-sectional study, 458 passengers of intercity bus terminals participated. The sampling method was multistage in this study. To collect data, a researcher-made questionnaire whose validity and reliability were confirmed was used. The collected data were analyzed using SPSS software version 22 and logistic regression test. Results: The study population included 47% of men and 53% of women with an average age of 28.35 ± 6.1. Police stations, high bus speeds, night, and inappropriate weather conditions were among the situations in which the use of a seatbelts was more on the bus. The results of the logistic regression test in the third model showed that perceived barriers, law and supervision, subjective norms, environment, and bus type predict the use of seat belts significantly (P <0.000). Conclusions: It seems that in addition to interventions designed to increase the knowledge and attitude of passengers and enhance the use of seatbelts on the bus, a special look is also needed at important people who influence the behavior of the people (subjective norms).","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132866729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Long-acting reversible contraceptive methods are the most effective, safe, convenient and cost-effective compared to other modern contraceptive methods. The prevalence of long-acting reversible contraceptive methods utilization among married women in the study area was only 5% out of users of all methods and eligible group, which is comparatively low compared to the regional and national targets. Objective: To identify determinants of long-acting reversible contraceptive methods utilization among married women of the reproductive age group in Ambo town, 2016 Methods: Community based unmatched case-control study was conducted in Ambo town among married women of reproductive age group. 140 users and 280 non-users were randomly included. Bivariable and multivariable logistic regression was used to identify candidate variables and independent predictor variables respectively. Adjusted odds ratios together with their corresponding 95% CI were calculated to assess the strength of association and statistical significance. Result: 139 users and 279 non-users were interviewed giving a response rate of 99%. Moderate level of knowledge on long-acting reversible contraceptive methods (AOR= 8.73, 95%CI: 3.08 - 24.77), Good level of knowledge (AOR=13.99, 95%CI: 4.93-39.71), spousal discussion on long-acting reversible contraceptive methods (AOR=2.88, 95%CI: 1.3-6.36), positive attitude toward long-acting reversible contraceptive methods (AOR=7.07, 95% CI: 3.77-13.24), Women from households in the poorest wealth quintile (AOR= 6.83, 95%CI: 2.68-17.38), Women from households in the medium wealth quintile (AOR=5.83, 95%CI: (2.23- 15.23) were positive determinants ,whereas intention to give birth in the future (AOR=0.09, 95%CI: 0.02- 0.36) and woman’s expectation of restriction to methods use (AOR=0.2, 95%CI: 0.08- 0.48) were negative determinants of long-acting reversible contraceptive methods utilization. Conclusion: Good knowledge, moderate knowledge, positive attitude, wealth index and spousal discussion were positive determinants, while intention to give birth in the future and woman’s expectation of restriction to method use were negative determinants of long-acting reversible contraceptive methods utilization. Stakeholders should emphasize behavioral change communication to increase knowledge on long-acting reversible contraceptive methods, strengthen inter-spousal discussion and bring a positive attitude toward long-acting reversible contraceptive methods. Keywords: long-acting reversible contraceptive methods, married women of reproductive age, Ambo town
{"title":"Determinants Of Long-Acting Reversible Contraceptive Methods Utilization Among Married Women Of Reproductive Age Group In Ambo Town, Oromia Region, West Ethiopia, 2016: A Case Control Study","authors":"D. R. Soboka, F. Tessema, Mamo Nigatu","doi":"10.21203/rs.2.12465/v1","DOIUrl":"https://doi.org/10.21203/rs.2.12465/v1","url":null,"abstract":"\u0000 Background: Long-acting reversible contraceptive methods are the most effective, safe, convenient and cost-effective compared to other modern contraceptive methods. The prevalence of long-acting reversible contraceptive methods utilization among married women in the study area was only 5% out of users of all methods and eligible group, which is comparatively low compared to the regional and national targets.\u0000Objective: To identify determinants of long-acting reversible contraceptive methods utilization among married women of the reproductive age group in Ambo town, 2016\u0000Methods: Community based unmatched case-control study was conducted in Ambo town among married women of reproductive age group. 140 users and 280 non-users were randomly included. Bivariable and multivariable logistic regression was used to identify candidate variables and independent predictor variables respectively. Adjusted odds ratios together with their corresponding 95% CI were calculated to assess the strength of association and statistical significance.\u0000Result: 139 users and 279 non-users were interviewed giving a response rate of 99%. Moderate level of knowledge on long-acting reversible contraceptive methods (AOR= 8.73, 95%CI: 3.08 - 24.77), Good level of knowledge (AOR=13.99, 95%CI: 4.93-39.71), spousal discussion on long-acting reversible contraceptive methods (AOR=2.88, 95%CI: 1.3-6.36), positive attitude toward long-acting reversible contraceptive methods (AOR=7.07, 95% CI: 3.77-13.24), Women from households in the poorest wealth quintile (AOR= 6.83, 95%CI: 2.68-17.38), Women from households in the medium wealth quintile (AOR=5.83, 95%CI: (2.23- 15.23) were positive determinants ,whereas intention to give birth in the future (AOR=0.09, 95%CI: 0.02- 0.36) and woman’s expectation of restriction to methods use (AOR=0.2, 95%CI: 0.08- 0.48) were negative determinants of long-acting reversible contraceptive methods utilization.\u0000Conclusion: Good knowledge, moderate knowledge, positive attitude, wealth index and spousal discussion were positive determinants, while intention to give birth in the future and woman’s expectation of restriction to method use were negative determinants of long-acting reversible contraceptive methods utilization.\u0000Stakeholders should emphasize behavioral change communication to increase knowledge on long-acting reversible contraceptive methods, strengthen inter-spousal discussion and bring a positive attitude toward long-acting reversible contraceptive methods.\u0000Keywords: long-acting reversible contraceptive methods, married women of reproductive age, Ambo town","PeriodicalId":319653,"journal":{"name":"Journal of Veterinary Health Science","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115286821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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