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Using plant extracts for the micropropagation of buckwheat 植物提取物在荞麦微繁中的应用
Pub Date : 2023-09-30 DOI: 10.18619/2072-9146-2023-5-37-42
S. A. Borovaya, N. G. Boginskaya, A. G. Klykov
Background . Various plant hormones are used (cytokinins, auxins) to increase the regeneration efficiency and the net reproduction rate of buckwheat in vitro . However, the growth and development rates of plantlets have been noted to be low under these conditions. For this reason, search for the plant extracts that are able to stimulate the regenerative ability of plants is a promising direction of biotechnological research. Materials and methods . Aseptic single-node cuttings of common buckwheat plantlets (varieties Dikul and Izumrud) were grown on MS nutrient media with plant extracts from Fagopyrum esculentum and Reynoutria japonica (0.1, 0.5, and 1%) for 21 days. The following morphobiological paramaters of the plantlets were evaluated: plant height, the number of internodes, the number of leaves, leaf length, and the number and length of roots. Results . Dealcoholized aqueous solutions of the extracts from F. esculentum and R. japonica in the studied concentrations (0.1-1%) significantly stimulated the growth and development of the buckwheat plantlets increasing their net reproduction rate (4.00-6.00) and rhizogenesis. The media with the plant extracts in concentrations of 0.1-0.5% were observed to produce the strongest positive effect. As the result, the morphobiological characteristics of the plantlets and the success rate of the micropropagation were the highest.
背景。利用多种植物激素(细胞分裂素、生长素)提高体外荞麦的再生效率和净繁殖率。然而,在这些条件下,植株的生长发育速度很低。因此,寻找能够刺激植物再生能力的植物提取物是生物技术研究的一个有前途的方向。材料和方法。将普通荞麦(Dikul和Izumrud品种)的无菌单节扦插在含有荞麦(Fagopyrum esculentum)和粳稻(Reynoutria japonica)植物提取物(0.1、0.5和1%)的MS营养培养基上培养21天。评价了植株的形态生物学参数:株高、节间数、叶数、叶长、根数和根长。结果。研究浓度(0.1 ~ 1%)下的荞麦提取物脱醇水溶液显著促进了荞麦植株的生长发育,提高了其净繁殖率(4.00 ~ 6.00)和根生。植物提取物浓度为0.1-0.5%的培养基产生的积极作用最强。结果表明,该植株的形态生物学特性和微繁成功率最高。
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引用次数: 0
Results of breeding work on cowpea-adzuki (Vigna angularis (Willd)) for the monsoonal climate of the Russian Far East 俄罗斯远东季风气候条件下豇豆(野生)选育工作结果
Pub Date : 2023-09-29 DOI: 10.18619/2072-9146-2023-5-24-27
A. S. Kornilov, N. A. Sakara, I. A. Vanyushkina
At the Primorskaya vegetable experimental station – branch of the Federal state budgetary scientific institution «Federal scientific vegetable center», selection and introduction into production of a new legume crop for Russia – adzuki cowpea. The varieties Asia (for universal cultivation) and Far East (for horticultural vegetable growing) were created and included in the State Register of the Russian Federation. Promising specimens have been created, which, in terms of their biological and economic indicators, are not inferior to the varieties Asia and Far East. Primary seed production of new varieties is underway and their introduction into farms and garden vegetable growing. The grain yield of cowpea-adzuki universal samples ranged from 3.15 t/ha (Asia variety, standard) to 2.77 t/ha (sample POOS 31-15). The highest grain productivity was observed in the standard (Asia - 22.5 g/plant) and in sample POOS 44-15 (20.8 g/plant). The Asia variety and the promising sample POOS 31-15 (20.2 pieces/plant) produce the most beans per plant. It was noted that the Dalnevostochnaya variety has the greatest resistance to cracking of beans during their ripening (7.2 points). Samples for universal cultivation have above average resistance to bean cracking (5.8-6.5 points). The plant height of promising samples is quite favorable for combine harvesting: 64 (POOS 31-15) – 71 (POOS 39-15) cm. Due to the fact that the flowering of cowpea-adzuki is extended, the ripening of beans is rarely 100%. For promising samples, this indicator is high and amounts to 95.5% (POOS 44-15) - 99.8% (POOS 31-15), which fully complies with the ATT requirements (more than 90%). The creation of varieties of a new vegetable crop for Russia will improve the economics of agricultural production in the difficult climatic conditions of the Far Eastern Federal District.
在滨海蔬菜试验站——联邦国家预算科学机构“联邦科学蔬菜中心”的分支机构,选择并引进了一种新的俄罗斯豆类作物——小豆。亚洲品种(用于普遍种植)和远东品种(用于园艺蔬菜种植)被创建并列入俄罗斯联邦国家登记册。在生物和经济指标方面,已培育出不逊于亚洲和远东品种的有前景的品种。新品种的初级种子生产正在进行中,并将其引入农场和花园蔬菜种植。豇豆-小豆通用样品的籽粒产量从3.15 t/ha(亚洲标准品种)到2.77 t/ha(样品POOS 31-15)不等。在标准(亚洲- 22.5 g/株)和样品POOS 44-15 (20.8 g/株)中观察到最高的粮食产量。亚洲品种和有前途的样品POOS 31-15(20.2粒/株)每株产量最多。据指出,Dalnevostochnaya品种在成熟过程中具有最大的抗开裂性(7.2分)。普遍栽培的样品抗豆裂性高于平均水平(5.8-6.5点)。有希望的样品株高为64 (POOS 31-15) - 71 (POOS 39-15) cm,非常适合联合收获。由于豇豆的开花时间较长,所以很少100%成熟。对于有希望的样品,该指标较高,达到95.5% (POOS 44-15) - 99.8% (POOS 31-15),完全符合ATT要求(90%以上)。为俄罗斯创造一种新的蔬菜作物品种将改善远东联邦区恶劣气候条件下农业生产的经济性。
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引用次数: 0
Priming – innovative development of methodology preparation of seeds for sowing (review) 启动——播种种子制备方法的创新发展(综述)
Pub Date : 2023-09-29 DOI: 10.18619/2072-9146-2023-05-28-36
A. V. Yanchenko, A. F. Bukharov, A. Y. Fedosov
The development of classical methods of soaking and growing seeds to the method of hydropriming and its further improvement based on the introduction of new knowledge and integrated approaches are shown. Priming is a controlled hydration of seeds (saturation with a limited amount of water), which causes the activation of metabolism, the initiation of germination (preventing the appearance of a root) and ensuring the subsequent reversibility of this process (by drying) while maintaining a stimulating effect. P.A. Genkel, who presented a thorough theoretical justification and detailed experimental confirmation of the effectiveness of pre-sowing soaking and subsequent drying (often multiple times) to increase drought and salt tolerance of plants. For a complex of methods for influencing seeds with water and osmotic solutions, W. Heidecker proposed the term priming, which is widely used in the English language, but at first did not take root in Russian literature. The characteristic of the internal reasons for the decrease in the viability of seeds, associated primarily with the aging process, during which the depletion of nutrient reserves, the accumulation of growth inhibitors, mutagens, reactive oxygen species and other harmful substances, the denaturation of proteins and nucleic acids, the oxidation of unsaturated lipids and external (abiotic and biotic), and their interactions. The article provides information about agents and methods, technical solutions and priming techniques. The role of water, conditions, factors, parameters, fundamental morpho-anatomical, physiological and biochemical substantiation of the germination process are described. The shortcomings of priming and ways to overcome them are outlined on the basis of an in-depth study of the metabolic processes occurring in the process of priming and subsequent germination in order to understand the mechanisms for increasing the sowing and productive properties of seeds. The necessity of maximizing the practice of applying priming, especially in difficult environmental conditions, was noted.
介绍了从传统的种子浸种法发展到水浸法的过程,以及在引入新知识和综合方法的基础上对水浸法的进一步改进。灌浆是一种控制种子水化(用有限的水饱和),它导致新陈代谢的激活,发芽的开始(防止根的出现),并确保这一过程的后续可逆性(通过干燥),同时保持刺激效果。P.A. Genkel,他提出了彻底的理论论证和详细的实验证实了播前浸泡和随后干燥(通常多次)提高植物耐旱性和耐盐性的有效性。对于用水和渗透溶液影响种子的一系列方法,W. Heidecker提出了“启动”(priming)一词,这个词在英语中广泛使用,但最初并没有在俄罗斯文学中扎根。种子活力下降的内在原因的特征,主要与衰老过程有关,在此过程中,营养储备的消耗,生长抑制剂,诱变剂,活性氧和其他有害物质的积累,蛋白质和核酸的变性,不饱和脂质的氧化和外部(非生物和生物),以及它们之间的相互作用。本文提供了有关代理和方法,技术解决方案和启动技术的信息。介绍了水的作用、条件、因素、参数、基本形态解剖、发芽过程的生理生化基础。本文在深入研究种子在萌发过程中发生的代谢过程的基础上,概述了启动的缺点和克服方法,以了解提高种子播种和生产性能的机制。有人指出,必须最大限度地使用引燃剂,特别是在困难的环境条件下。
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引用次数: 0
The genetics of fruit color in Cucurbita pepo L. 瓜类果实颜色的遗传学研究。
Pub Date : 2023-09-29 DOI: 10.18619/2072-9146-2023-5-18-23
I. B. Korottseva, A. S. Ermolaev, G. A. Khimich
Species Cucurbita pep o L., which includes marrow, squash, hard-barked pumpkin and a number of other crops, has a high polymorphism both in plant structure and in shape, size, color of the bark and fruit pulp. The color of the bark of the fruits of this species can be white, cream, yellow, green, orange with or without a pattern. Appearance can greatly influence the marketing success of new hybrids among the target audience. This is explained by the fact that there are strong regional consumer preferences regarding the color of fruits, although most often, on the shelves of chain stores, in fresh or canned form, you can find fruits of bright, saturated colors and shades. At the same time, brightly colored fruits are more often used for canning, while light-colored ones are used for processing into caviar [1]. Paris H.S. and Brown R.N. summarized the identification of more than 80 gene loci that affect fruit color in Cucurbita pepo L., some of which are multi-allelic [2]. The color of the fruit in hard-barked pumpkin depends on the presence and interaction between the loci responsible for this trait, and many other factors, including growing conditions [3,4]. In cucurbits, in different phenophases, different genes are responsible for color [5–7]. All this makes the selection of summer squash and scallop on the basis of the color of the fruit bark very difficult. Knowing the genetics of coloration can make this task easier. In this article, we tried to summarize the results of studies presented in foreign literature sources on the study of Cucurbita pepo L. genes responsible for the color of the surface of the fruit and pulp, its intensity, the presence or absence of a pattern of various colors and configurations. No domestic studies on this topic could be found.
葫芦属(Cucurbita pep o L.)包括西葫芦、南瓜、硬皮南瓜等多种作物,在植物结构、形状、大小、树皮和果肉颜色等方面都具有高度的多态性。这种水果的树皮颜色可以是白色、奶油色、黄色、绿色、橙色,有或没有图案。外观可以极大地影响新混合动力车在目标受众中的营销成功。这可以用这样一个事实来解释:尽管在连锁店的货架上,新鲜或罐装的水果通常都是明亮、饱和的颜色和色调,但区域消费者对水果的颜色有很强的偏好。同时,颜色鲜艳的水果多用于罐装,而浅色的水果多用于加工成鱼子酱[1]。Paris H.S.和Brown R.N.总结了影响瓜类果实颜色的80多个基因位点的鉴定,其中一些是多等位基因[2]。硬皮南瓜果实的颜色取决于负责该性状的基因座的存在和相互作用,以及许多其他因素,包括生长条件[3,4]。在葫芦中,在不同的物候期,不同的基因负责颜色[5-7]。这使得根据果皮颜色选择夏南瓜和扇贝非常困难。了解颜色的遗传学可以使这项任务变得更容易。在本文中,我们试图总结在国外文献来源的研究结果,负责葫芦的果实和果肉表面的颜色,其强度,存在或不存在各种颜色和配置图案的基因的研究。国内尚无关于该课题的研究。
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引用次数: 0
The current state of technological support for vegetable crops production in the Russian Federation 俄罗斯联邦蔬菜作物生产技术支助的现状
Pub Date : 2023-09-29 DOI: 10.18619/2072-9146-2023-5-5-17
Ya. P. Lobachevsky, A. S. Dorokhov, A. V. Sibirev
The main limiting factor in the development of the vegetable growing industry is the low technical level of vegetable cultivation. It should be noted that many works in domestic breeding, as well as in seed production of vegetable crops, are carried out manually. The decrease in the share of Russian varieties in the market of seed material of vegetable crops is largely due to the fact that the technological level and technical equipment of most originating institutions of Russian varieties are simply not comparable with the level of modern Western European breeding and seed production centers and companies that seek to increase the supply of seed material to the Russian market. One of the significant factors in increasing the efficiency of vegetable production is the use of highly efficient agricultural machinery and equipment. At the moment, there are no machines for performing the main operations of cultivating vegetable products in Russia, and the available agricultural machines for vegetable producers are on the verge of physical wear and tear and have long ago exhausted their depreciation resource. The existing foreign analogues presented on the Russian market do not fully meet the agrotechnical requirements imposed by Russian farmers on foreign-made machines. Based on the analysis of the technical support of the vegetable growing industry in the Russian Federation, the need for commodity producers in modern hightech complexes of machines for the production of vegetable crops is economically determined based on the total sown area of vegetable crops. The results of the statistical studies carried out made it possible to assess the current state of agricultural engineering for the production of the required number of machines for vegetable growing. The increase in sown (planting) areas and the expected increase in the production of the main types of crop products against the background of a decrease in the availability of agricultural machinery contributes to an increase in the load per unit of equipment and creates risks of a decrease in the level of agricultural mechanization. Currently, low volumes of domestic seed production are associated, among other things, with the low level of technical equipment of breeding and seed-growing organizations with specialized machines and equipment.
蔬菜种植技术水平低是制约蔬菜种植业发展的主要因素。应该指出的是,国内育种以及蔬菜作物制种的许多工作都是手工进行的。俄罗斯品种在蔬菜作物种子材料市场上所占份额的下降,主要是由于大多数俄罗斯品种原产机构的技术水平和技术设备根本无法与现代西欧育种和种子生产中心以及寻求增加向俄罗斯市场供应种子材料的公司的水平相比。提高蔬菜生产效率的重要因素之一是使用高效的农业机械设备。目前,俄罗斯没有从事蔬菜产品种植主要作业的机器,可供蔬菜生产者使用的农机已经处于物理磨损的边缘,早已耗尽了折旧资源。俄罗斯市场上现有的国外类似产品不能完全满足俄罗斯农民对外国制造机器的农业技术要求。根据对俄罗斯联邦蔬菜种植业技术支持的分析,对蔬菜作物生产的现代高科技机器综合体的商品生产者的需求是根据蔬菜作物的总播种面积在经济上确定的。所进行的统计研究的结果使评估生产所需数量的蔬菜种植机器的农业工程的现状成为可能。在农业机械可用性减少的背景下,播种(种植)面积的增加和主要作物产品产量的预期增加导致单位设备负荷的增加,并造成农业机械化水平下降的风险。目前,国内种子产量低,除其他外,与育种和种子种植组织拥有专门机器和设备的技术设备水平低有关。
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