Pub Date : 2023-09-30DOI: 10.18619/2072-9146-2023-5-37-42
S. A. Borovaya, N. G. Boginskaya, A. G. Klykov
Background . Various plant hormones are used (cytokinins, auxins) to increase the regeneration efficiency and the net reproduction rate of buckwheat in vitro . However, the growth and development rates of plantlets have been noted to be low under these conditions. For this reason, search for the plant extracts that are able to stimulate the regenerative ability of plants is a promising direction of biotechnological research. Materials and methods . Aseptic single-node cuttings of common buckwheat plantlets (varieties Dikul and Izumrud) were grown on MS nutrient media with plant extracts from Fagopyrum esculentum and Reynoutria japonica (0.1, 0.5, and 1%) for 21 days. The following morphobiological paramaters of the plantlets were evaluated: plant height, the number of internodes, the number of leaves, leaf length, and the number and length of roots. Results . Dealcoholized aqueous solutions of the extracts from F. esculentum and R. japonica in the studied concentrations (0.1-1%) significantly stimulated the growth and development of the buckwheat plantlets increasing their net reproduction rate (4.00-6.00) and rhizogenesis. The media with the plant extracts in concentrations of 0.1-0.5% were observed to produce the strongest positive effect. As the result, the morphobiological characteristics of the plantlets and the success rate of the micropropagation were the highest.
{"title":"Using plant extracts for the micropropagation of buckwheat","authors":"S. A. Borovaya, N. G. Boginskaya, A. G. Klykov","doi":"10.18619/2072-9146-2023-5-37-42","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-5-37-42","url":null,"abstract":"Background . Various plant hormones are used (cytokinins, auxins) to increase the regeneration efficiency and the net reproduction rate of buckwheat in vitro . However, the growth and development rates of plantlets have been noted to be low under these conditions. For this reason, search for the plant extracts that are able to stimulate the regenerative ability of plants is a promising direction of biotechnological research. Materials and methods . Aseptic single-node cuttings of common buckwheat plantlets (varieties Dikul and Izumrud) were grown on MS nutrient media with plant extracts from Fagopyrum esculentum and Reynoutria japonica (0.1, 0.5, and 1%) for 21 days. The following morphobiological paramaters of the plantlets were evaluated: plant height, the number of internodes, the number of leaves, leaf length, and the number and length of roots. Results . Dealcoholized aqueous solutions of the extracts from F. esculentum and R. japonica in the studied concentrations (0.1-1%) significantly stimulated the growth and development of the buckwheat plantlets increasing their net reproduction rate (4.00-6.00) and rhizogenesis. The media with the plant extracts in concentrations of 0.1-0.5% were observed to produce the strongest positive effect. As the result, the morphobiological characteristics of the plantlets and the success rate of the micropropagation were the highest.","PeriodicalId":32278,"journal":{"name":"Ovosi Rossii","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136344664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-29DOI: 10.18619/2072-9146-2023-5-24-27
A. S. Kornilov, N. A. Sakara, I. A. Vanyushkina
At the Primorskaya vegetable experimental station – branch of the Federal state budgetary scientific institution «Federal scientific vegetable center», selection and introduction into production of a new legume crop for Russia – adzuki cowpea. The varieties Asia (for universal cultivation) and Far East (for horticultural vegetable growing) were created and included in the State Register of the Russian Federation. Promising specimens have been created, which, in terms of their biological and economic indicators, are not inferior to the varieties Asia and Far East. Primary seed production of new varieties is underway and their introduction into farms and garden vegetable growing. The grain yield of cowpea-adzuki universal samples ranged from 3.15 t/ha (Asia variety, standard) to 2.77 t/ha (sample POOS 31-15). The highest grain productivity was observed in the standard (Asia - 22.5 g/plant) and in sample POOS 44-15 (20.8 g/plant). The Asia variety and the promising sample POOS 31-15 (20.2 pieces/plant) produce the most beans per plant. It was noted that the Dalnevostochnaya variety has the greatest resistance to cracking of beans during their ripening (7.2 points). Samples for universal cultivation have above average resistance to bean cracking (5.8-6.5 points). The plant height of promising samples is quite favorable for combine harvesting: 64 (POOS 31-15) – 71 (POOS 39-15) cm. Due to the fact that the flowering of cowpea-adzuki is extended, the ripening of beans is rarely 100%. For promising samples, this indicator is high and amounts to 95.5% (POOS 44-15) - 99.8% (POOS 31-15), which fully complies with the ATT requirements (more than 90%). The creation of varieties of a new vegetable crop for Russia will improve the economics of agricultural production in the difficult climatic conditions of the Far Eastern Federal District.
{"title":"Results of breeding work on cowpea-adzuki (Vigna angularis (Willd)) for the monsoonal climate of the Russian Far East","authors":"A. S. Kornilov, N. A. Sakara, I. A. Vanyushkina","doi":"10.18619/2072-9146-2023-5-24-27","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-5-24-27","url":null,"abstract":"At the Primorskaya vegetable experimental station – branch of the Federal state budgetary scientific institution «Federal scientific vegetable center», selection and introduction into production of a new legume crop for Russia – adzuki cowpea. The varieties Asia (for universal cultivation) and Far East (for horticultural vegetable growing) were created and included in the State Register of the Russian Federation. Promising specimens have been created, which, in terms of their biological and economic indicators, are not inferior to the varieties Asia and Far East. Primary seed production of new varieties is underway and their introduction into farms and garden vegetable growing. The grain yield of cowpea-adzuki universal samples ranged from 3.15 t/ha (Asia variety, standard) to 2.77 t/ha (sample POOS 31-15). The highest grain productivity was observed in the standard (Asia - 22.5 g/plant) and in sample POOS 44-15 (20.8 g/plant). The Asia variety and the promising sample POOS 31-15 (20.2 pieces/plant) produce the most beans per plant. It was noted that the Dalnevostochnaya variety has the greatest resistance to cracking of beans during their ripening (7.2 points). Samples for universal cultivation have above average resistance to bean cracking (5.8-6.5 points). The plant height of promising samples is quite favorable for combine harvesting: 64 (POOS 31-15) – 71 (POOS 39-15) cm. Due to the fact that the flowering of cowpea-adzuki is extended, the ripening of beans is rarely 100%. For promising samples, this indicator is high and amounts to 95.5% (POOS 44-15) - 99.8% (POOS 31-15), which fully complies with the ATT requirements (more than 90%). The creation of varieties of a new vegetable crop for Russia will improve the economics of agricultural production in the difficult climatic conditions of the Far Eastern Federal District.","PeriodicalId":32278,"journal":{"name":"Ovosi Rossii","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135248144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-29DOI: 10.18619/2072-9146-2023-05-28-36
A. V. Yanchenko, A. F. Bukharov, A. Y. Fedosov
The development of classical methods of soaking and growing seeds to the method of hydropriming and its further improvement based on the introduction of new knowledge and integrated approaches are shown. Priming is a controlled hydration of seeds (saturation with a limited amount of water), which causes the activation of metabolism, the initiation of germination (preventing the appearance of a root) and ensuring the subsequent reversibility of this process (by drying) while maintaining a stimulating effect. P.A. Genkel, who presented a thorough theoretical justification and detailed experimental confirmation of the effectiveness of pre-sowing soaking and subsequent drying (often multiple times) to increase drought and salt tolerance of plants. For a complex of methods for influencing seeds with water and osmotic solutions, W. Heidecker proposed the term priming, which is widely used in the English language, but at first did not take root in Russian literature. The characteristic of the internal reasons for the decrease in the viability of seeds, associated primarily with the aging process, during which the depletion of nutrient reserves, the accumulation of growth inhibitors, mutagens, reactive oxygen species and other harmful substances, the denaturation of proteins and nucleic acids, the oxidation of unsaturated lipids and external (abiotic and biotic), and their interactions. The article provides information about agents and methods, technical solutions and priming techniques. The role of water, conditions, factors, parameters, fundamental morpho-anatomical, physiological and biochemical substantiation of the germination process are described. The shortcomings of priming and ways to overcome them are outlined on the basis of an in-depth study of the metabolic processes occurring in the process of priming and subsequent germination in order to understand the mechanisms for increasing the sowing and productive properties of seeds. The necessity of maximizing the practice of applying priming, especially in difficult environmental conditions, was noted.
{"title":"Priming – innovative development of methodology preparation of seeds for sowing (review)","authors":"A. V. Yanchenko, A. F. Bukharov, A. Y. Fedosov","doi":"10.18619/2072-9146-2023-05-28-36","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-05-28-36","url":null,"abstract":"The development of classical methods of soaking and growing seeds to the method of hydropriming and its further improvement based on the introduction of new knowledge and integrated approaches are shown. Priming is a controlled hydration of seeds (saturation with a limited amount of water), which causes the activation of metabolism, the initiation of germination (preventing the appearance of a root) and ensuring the subsequent reversibility of this process (by drying) while maintaining a stimulating effect. P.A. Genkel, who presented a thorough theoretical justification and detailed experimental confirmation of the effectiveness of pre-sowing soaking and subsequent drying (often multiple times) to increase drought and salt tolerance of plants. For a complex of methods for influencing seeds with water and osmotic solutions, W. Heidecker proposed the term priming, which is widely used in the English language, but at first did not take root in Russian literature. The characteristic of the internal reasons for the decrease in the viability of seeds, associated primarily with the aging process, during which the depletion of nutrient reserves, the accumulation of growth inhibitors, mutagens, reactive oxygen species and other harmful substances, the denaturation of proteins and nucleic acids, the oxidation of unsaturated lipids and external (abiotic and biotic), and their interactions. The article provides information about agents and methods, technical solutions and priming techniques. The role of water, conditions, factors, parameters, fundamental morpho-anatomical, physiological and biochemical substantiation of the germination process are described. The shortcomings of priming and ways to overcome them are outlined on the basis of an in-depth study of the metabolic processes occurring in the process of priming and subsequent germination in order to understand the mechanisms for increasing the sowing and productive properties of seeds. The necessity of maximizing the practice of applying priming, especially in difficult environmental conditions, was noted.","PeriodicalId":32278,"journal":{"name":"Ovosi Rossii","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135247994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-29DOI: 10.18619/2072-9146-2023-5-18-23
I. B. Korottseva, A. S. Ermolaev, G. A. Khimich
Species Cucurbita pep o L., which includes marrow, squash, hard-barked pumpkin and a number of other crops, has a high polymorphism both in plant structure and in shape, size, color of the bark and fruit pulp. The color of the bark of the fruits of this species can be white, cream, yellow, green, orange with or without a pattern. Appearance can greatly influence the marketing success of new hybrids among the target audience. This is explained by the fact that there are strong regional consumer preferences regarding the color of fruits, although most often, on the shelves of chain stores, in fresh or canned form, you can find fruits of bright, saturated colors and shades. At the same time, brightly colored fruits are more often used for canning, while light-colored ones are used for processing into caviar [1]. Paris H.S. and Brown R.N. summarized the identification of more than 80 gene loci that affect fruit color in Cucurbita pepo L., some of which are multi-allelic [2]. The color of the fruit in hard-barked pumpkin depends on the presence and interaction between the loci responsible for this trait, and many other factors, including growing conditions [3,4]. In cucurbits, in different phenophases, different genes are responsible for color [5–7]. All this makes the selection of summer squash and scallop on the basis of the color of the fruit bark very difficult. Knowing the genetics of coloration can make this task easier. In this article, we tried to summarize the results of studies presented in foreign literature sources on the study of Cucurbita pepo L. genes responsible for the color of the surface of the fruit and pulp, its intensity, the presence or absence of a pattern of various colors and configurations. No domestic studies on this topic could be found.
葫芦属(Cucurbita pep o L.)包括西葫芦、南瓜、硬皮南瓜等多种作物,在植物结构、形状、大小、树皮和果肉颜色等方面都具有高度的多态性。这种水果的树皮颜色可以是白色、奶油色、黄色、绿色、橙色,有或没有图案。外观可以极大地影响新混合动力车在目标受众中的营销成功。这可以用这样一个事实来解释:尽管在连锁店的货架上,新鲜或罐装的水果通常都是明亮、饱和的颜色和色调,但区域消费者对水果的颜色有很强的偏好。同时,颜色鲜艳的水果多用于罐装,而浅色的水果多用于加工成鱼子酱[1]。Paris H.S.和Brown R.N.总结了影响瓜类果实颜色的80多个基因位点的鉴定,其中一些是多等位基因[2]。硬皮南瓜果实的颜色取决于负责该性状的基因座的存在和相互作用,以及许多其他因素,包括生长条件[3,4]。在葫芦中,在不同的物候期,不同的基因负责颜色[5-7]。这使得根据果皮颜色选择夏南瓜和扇贝非常困难。了解颜色的遗传学可以使这项任务变得更容易。在本文中,我们试图总结在国外文献来源的研究结果,负责葫芦的果实和果肉表面的颜色,其强度,存在或不存在各种颜色和配置图案的基因的研究。国内尚无关于该课题的研究。
{"title":"The genetics of fruit color in Cucurbita pepo L.","authors":"I. B. Korottseva, A. S. Ermolaev, G. A. Khimich","doi":"10.18619/2072-9146-2023-5-18-23","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-5-18-23","url":null,"abstract":"Species Cucurbita pep o L., which includes marrow, squash, hard-barked pumpkin and a number of other crops, has a high polymorphism both in plant structure and in shape, size, color of the bark and fruit pulp. The color of the bark of the fruits of this species can be white, cream, yellow, green, orange with or without a pattern. Appearance can greatly influence the marketing success of new hybrids among the target audience. This is explained by the fact that there are strong regional consumer preferences regarding the color of fruits, although most often, on the shelves of chain stores, in fresh or canned form, you can find fruits of bright, saturated colors and shades. At the same time, brightly colored fruits are more often used for canning, while light-colored ones are used for processing into caviar [1]. Paris H.S. and Brown R.N. summarized the identification of more than 80 gene loci that affect fruit color in Cucurbita pepo L., some of which are multi-allelic [2]. The color of the fruit in hard-barked pumpkin depends on the presence and interaction between the loci responsible for this trait, and many other factors, including growing conditions [3,4]. In cucurbits, in different phenophases, different genes are responsible for color [5–7]. All this makes the selection of summer squash and scallop on the basis of the color of the fruit bark very difficult. Knowing the genetics of coloration can make this task easier. In this article, we tried to summarize the results of studies presented in foreign literature sources on the study of Cucurbita pepo L. genes responsible for the color of the surface of the fruit and pulp, its intensity, the presence or absence of a pattern of various colors and configurations. No domestic studies on this topic could be found.","PeriodicalId":32278,"journal":{"name":"Ovosi Rossii","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135249121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-29DOI: 10.18619/2072-9146-2023-5-5-17
Ya. P. Lobachevsky, A. S. Dorokhov, A. V. Sibirev
The main limiting factor in the development of the vegetable growing industry is the low technical level of vegetable cultivation. It should be noted that many works in domestic breeding, as well as in seed production of vegetable crops, are carried out manually. The decrease in the share of Russian varieties in the market of seed material of vegetable crops is largely due to the fact that the technological level and technical equipment of most originating institutions of Russian varieties are simply not comparable with the level of modern Western European breeding and seed production centers and companies that seek to increase the supply of seed material to the Russian market. One of the significant factors in increasing the efficiency of vegetable production is the use of highly efficient agricultural machinery and equipment. At the moment, there are no machines for performing the main operations of cultivating vegetable products in Russia, and the available agricultural machines for vegetable producers are on the verge of physical wear and tear and have long ago exhausted their depreciation resource. The existing foreign analogues presented on the Russian market do not fully meet the agrotechnical requirements imposed by Russian farmers on foreign-made machines. Based on the analysis of the technical support of the vegetable growing industry in the Russian Federation, the need for commodity producers in modern hightech complexes of machines for the production of vegetable crops is economically determined based on the total sown area of vegetable crops. The results of the statistical studies carried out made it possible to assess the current state of agricultural engineering for the production of the required number of machines for vegetable growing. The increase in sown (planting) areas and the expected increase in the production of the main types of crop products against the background of a decrease in the availability of agricultural machinery contributes to an increase in the load per unit of equipment and creates risks of a decrease in the level of agricultural mechanization. Currently, low volumes of domestic seed production are associated, among other things, with the low level of technical equipment of breeding and seed-growing organizations with specialized machines and equipment.
{"title":"The current state of technological support for vegetable crops production in the Russian Federation","authors":"Ya. P. Lobachevsky, A. S. Dorokhov, A. V. Sibirev","doi":"10.18619/2072-9146-2023-5-5-17","DOIUrl":"https://doi.org/10.18619/2072-9146-2023-5-5-17","url":null,"abstract":"The main limiting factor in the development of the vegetable growing industry is the low technical level of vegetable cultivation. It should be noted that many works in domestic breeding, as well as in seed production of vegetable crops, are carried out manually. The decrease in the share of Russian varieties in the market of seed material of vegetable crops is largely due to the fact that the technological level and technical equipment of most originating institutions of Russian varieties are simply not comparable with the level of modern Western European breeding and seed production centers and companies that seek to increase the supply of seed material to the Russian market. One of the significant factors in increasing the efficiency of vegetable production is the use of highly efficient agricultural machinery and equipment. At the moment, there are no machines for performing the main operations of cultivating vegetable products in Russia, and the available agricultural machines for vegetable producers are on the verge of physical wear and tear and have long ago exhausted their depreciation resource. The existing foreign analogues presented on the Russian market do not fully meet the agrotechnical requirements imposed by Russian farmers on foreign-made machines. Based on the analysis of the technical support of the vegetable growing industry in the Russian Federation, the need for commodity producers in modern hightech complexes of machines for the production of vegetable crops is economically determined based on the total sown area of vegetable crops. The results of the statistical studies carried out made it possible to assess the current state of agricultural engineering for the production of the required number of machines for vegetable growing. The increase in sown (planting) areas and the expected increase in the production of the main types of crop products against the background of a decrease in the availability of agricultural machinery contributes to an increase in the load per unit of equipment and creates risks of a decrease in the level of agricultural mechanization. Currently, low volumes of domestic seed production are associated, among other things, with the low level of technical equipment of breeding and seed-growing organizations with specialized machines and equipment.","PeriodicalId":32278,"journal":{"name":"Ovosi Rossii","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135249123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}