Purpose - to determine the presence of genetic determinism on the part of thrombophilia and angiogenesis candidate genes, their prognostic value in the development of preeclampsia (РЕ) in women with retrochorial hematoma (RCH). Materials and methods. The course of pregnancy was analyzed in 137 women with the threat of miscarriage aged 20 to 47 years (the Group 1 - 60 patients with RCH, the Group 2 - 77 patients without hematoma). Additionally, polymorphisms of the thrombophilia genes, the vascular endothelial growth factor gene - A (VEGF-A-634C>G) and the nitric oxide synthase 3 gene (NOS3 -786 C>T) were studied. To assess the correlation dependencies, the water-soluble tyrosine kinase-1 (sFlt-1) / placental growth factor (PlGF) (sFlt-1/PlGF) ratio was determined using TRACE-technologe (BRAHSMS Kryptor). Results. The average age of women in the Group 1 was 31.2±0.6 (95% CI: 30.0-32.4) years, in the Group 2 was 32.2±0.6 (95% CI: 31.0-33.3) years (p=0.243 by t-criterion). The calculation of the risk of developing PE in women of both groups established a high risk of its development in 28 (46.7%) and 23 (29.9%), respectively (p=0.044 by 2). Significant correlations in pairs were revealed: F13A1 gene polymorphism (9 G>T) - a decrease in the level of PlGF (rs=-0.296; p=0.023) and an increase in the value of sFlt-1/PlGF at 32-33+6 weeks (rs=0.248; p=0.050); FGB-fibrinogen gene (455 G>A) - the presence of chronic arterial hypertension (CAH) in a pregnant woman (rs=0.260, p=0.002), an increased risk of developing PE in the trimester I (rs=0.180; p=0.038); PAI-1 gene - reduced level of sFlt-1 (rs=-0.331; p=0.010) and sFlt-1/PlGF at 32-33+6 weeks (rs=-0.310; p=0.015); ITGB3-β gene - the presence of CAH in a pregnant woman (rs=0.176; p=0.040), an increase in sFlt-1/PlGF (rs=0.221; p=0.087). There was a trend towards an increased risk of developing PE in the first trimester in carriers of the VEGF-A gene polymorphism (-634 C>G) (rs=0.226; p=0.083). NOS3 gene polymorphism was associated with increased sFlt-1 (rs=0.252; p=0.063) and sFlt-1/PlGF ratio at 32-33+6 weeks (rs=0.239; p=0.079). Conclusions. Calculation of the risk of developing PE in women with RCH found a high risk of its development. The presence of VEGF-A polymorphism (-634 C>G) leads to an increased risk of developing PE and other placenta-associated complications. The presence of the NOS3 gene polymorphism (-786 C>T) affects the level of sFlt-1, leading to its increase and a change in the level of the sFlt-1/PlGF ratio at 32-33+6 weeks, which indicates an increased risk of developing late PE. The best predictor value has a combination of studying not only clinical data and biochemical parameters, but also genetic determinism. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No confl
{"title":"Assessment of genetic susceptibility to the occurrence of placenta-associated complications in pregnant women with retrochorial hematomas in the trimester I of pregnancy","authors":"M. Yushchenko, Y. Duka","doi":"10.15574/pp.2023.93.12","DOIUrl":"https://doi.org/10.15574/pp.2023.93.12","url":null,"abstract":"Purpose - to determine the presence of genetic determinism on the part of thrombophilia and angiogenesis candidate genes, their prognostic value in the development of preeclampsia (РЕ) in women with retrochorial hematoma (RCH). Materials and methods. The course of pregnancy was analyzed in 137 women with the threat of miscarriage aged 20 to 47 years (the Group 1 - 60 patients with RCH, the Group 2 - 77 patients without hematoma). Additionally, polymorphisms of the thrombophilia genes, the vascular endothelial growth factor gene - A (VEGF-A-634C>G) and the nitric oxide synthase 3 gene (NOS3 -786 C>T) were studied. To assess the correlation dependencies, the water-soluble tyrosine kinase-1 (sFlt-1) / placental growth factor (PlGF) (sFlt-1/PlGF) ratio was determined using TRACE-technologe (BRAHSMS Kryptor). Results. The average age of women in the Group 1 was 31.2±0.6 (95% CI: 30.0-32.4) years, in the Group 2 was 32.2±0.6 (95% CI: 31.0-33.3) years (p=0.243 by t-criterion). The calculation of the risk of developing PE in women of both groups established a high risk of its development in 28 (46.7%) and 23 (29.9%), respectively (p=0.044 by 2). Significant correlations in pairs were revealed: F13A1 gene polymorphism (9 G>T) - a decrease in the level of PlGF (rs=-0.296; p=0.023) and an increase in the value of sFlt-1/PlGF at 32-33+6 weeks (rs=0.248; p=0.050); FGB-fibrinogen gene (455 G>A) - the presence of chronic arterial hypertension (CAH) in a pregnant woman (rs=0.260, p=0.002), an increased risk of developing PE in the trimester I (rs=0.180; p=0.038); PAI-1 gene - reduced level of sFlt-1 (rs=-0.331; p=0.010) and sFlt-1/PlGF at 32-33+6 weeks (rs=-0.310; p=0.015); ITGB3-β gene - the presence of CAH in a pregnant woman (rs=0.176; p=0.040), an increase in sFlt-1/PlGF (rs=0.221; p=0.087). There was a trend towards an increased risk of developing PE in the first trimester in carriers of the VEGF-A gene polymorphism (-634 C>G) (rs=0.226; p=0.083). NOS3 gene polymorphism was associated with increased sFlt-1 (rs=0.252; p=0.063) and sFlt-1/PlGF ratio at 32-33+6 weeks (rs=0.239; p=0.079). Conclusions. Calculation of the risk of developing PE in women with RCH found a high risk of its development. The presence of VEGF-A polymorphism (-634 C>G) leads to an increased risk of developing PE and other placenta-associated complications. The presence of the NOS3 gene polymorphism (-786 C>T) affects the level of sFlt-1, leading to its increase and a change in the level of the sFlt-1/PlGF ratio at 32-33+6 weeks, which indicates an increased risk of developing late PE. The best predictor value has a combination of studying not only clinical data and biochemical parameters, but also genetic determinism. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No confl","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"468 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121794222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose - to reveal the features of the course of the disease, childbirth, and the postpartum period in patients after assisted reproductive technologies (ART) against the background of sexually transmitted infections in the anamnesis. Materials and methods. 132 women with infertility on the background of sexually transmitted infections (STIs) in the anamnesis standard preparation for the ART program was carried out, of which 55 women completed the ART program with pregnancy and were included in the main group. The control group is 30 pregnant women without a period of infertility who became pregnant on their own. Statistical processing of research results was carried out using standard programs Microsoft Excel 5.0 and Statistica 8. Results. Pregnancy and childbirth in women after ART with a history of STIs occurs with a significantly high frequency of the following complications: threat of termination of pregnancy - 9 (16.4%); reproductive losses in the first half of pregnancy - 10 (18.2%); carrier of chronic viral and bacterial infection in the first half of pregnancy - in all women; early development of preeclampsia - 6 (13.3%); placental dysfunction - 26 (57.8%); fetal growth retardation syndrome - 17 (37.8%); fetal distress - 10 (22.2%); premature birth - 12 (26.7%); and high frequency of caesarean section - 40 (88.9%). Conclusions. The obtained results indicate that the development, implementation and application of an improved algorithm of pre-gravid preparation in women with a history of STIs before the ART program is relevant, which, in our opinion, can have a positive effect on the obstetric consequences of the delivery of these women. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
{"title":"Clinical course of pregnancy and delivery after assisted reproductive technologies in the background of sexually transmitted infections","authors":"O. Krotik","doi":"10.15574/pp.2023.93.19","DOIUrl":"https://doi.org/10.15574/pp.2023.93.19","url":null,"abstract":"Purpose - to reveal the features of the course of the disease, childbirth, and the postpartum period in patients after assisted reproductive technologies (ART) against the background of sexually transmitted infections in the anamnesis. Materials and methods. 132 women with infertility on the background of sexually transmitted infections (STIs) in the anamnesis standard preparation for the ART program was carried out, of which 55 women completed the ART program with pregnancy and were included in the main group. The control group is 30 pregnant women without a period of infertility who became pregnant on their own. Statistical processing of research results was carried out using standard programs Microsoft Excel 5.0 and Statistica 8. Results. Pregnancy and childbirth in women after ART with a history of STIs occurs with a significantly high frequency of the following complications: threat of termination of pregnancy - 9 (16.4%); reproductive losses in the first half of pregnancy - 10 (18.2%); carrier of chronic viral and bacterial infection in the first half of pregnancy - in all women; early development of preeclampsia - 6 (13.3%); placental dysfunction - 26 (57.8%); fetal growth retardation syndrome - 17 (37.8%); fetal distress - 10 (22.2%); premature birth - 12 (26.7%); and high frequency of caesarean section - 40 (88.9%). Conclusions. The obtained results indicate that the development, implementation and application of an improved algorithm of pre-gravid preparation in women with a history of STIs before the ART program is relevant, which, in our opinion, can have a positive effect on the obstetric consequences of the delivery of these women. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115945201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Atopic march (AM) occurs as the linear progression of atopic disorders (AtD) from atopic dermatitis (AD) phenotype to its combinations with other AtD: allergic rhinitis/rhinoconjunctivitis (AR/ARC) and bronchial asthma (BA). Thymic stromal lymphopoietin (TSLP) plays an important role in AtD pathogenesis. Single nucleotide variant (SNV) rs11466749 of the TSLP gene during the last decades showed the controversial roles of A/A, A/G and G/G genotypes in occurrence of the mono- and polyorgan AM phenotypes in children. Purpose - to determine the associations and risks of AM phenotypes with homozygous or heterozygous SNV rs11466749 genotypes of the TSLP gene in children. Materials and methods. The study involved 398 children aged 3 to 18 years old: 293 children in the main group and 105 - in the control group. Patients of the main group suffered from AtD in 6 different AM phenotypes: «AD», «ARARC», «BA», «AD+AR/ARC», «BA+AR/ARC», «AD+AR/ARC+BA»; patients of the control group suffered from gastrointestinal pathology. Patients of all cohorts were genotyped for genotype variants A/A, A/G and G/G rs11466749 TSLP by the polymerase chain reaction in real time. Pearson’ contingency coefficient (rb), Pearson test (ꭕ2), Fisher’s exact test, odds ratio (OR) with a 95% confidence interval (95% CI) were used for statistical processing of the obtained results. Results at p≤0.05 were considered statistically significant. Results. The homozygous A/A rs11466749 TSLP genotype was significantly most frequent in phenotypes with mono- or oligoorgan affection «AR/ARC» and polyorgan affection «BA+AR/ARC» and «AD+AR/ARC+BA»: 66,2%, 65.3% and 76.9% respectively (p<0.05). Heterozygous genotype A/G rs11466749 TSLP was the second most significant and frequent: «AR/ARC» - 31.0% (p<0.05), «BA+AR/ARC» - 31.9% (p=0.05-0.1) and «AD+AR/ARC+BA» - 11.5% (p<0.05). Genotype A/A rs11466749 TSLP was significantly associated and increased the development risks of the 3 specified AM phenotypes: «AR/ARC» - rb=0.156, OR=1.92 (95% CI: 1.03-3.58, p<0.05); «BA+AR/ARC» - rb=0.147, OR=1.84 (95% CI: 1.0-3.42, p<0.05); «AD+AR/ARC+BA» - rb=0.212, OR=3.27 (95% CI: 1.22-8.80, p<0.05). Genotype A/G rs11466749 TSLP was reliably associated and had a protective effect on the development of bespoke AM phenotypes: «AR/ARC» - rb=0.148, OR=0.53 (95% CI: 0.28-1.0, p<0.05); «BA+AR/ARC2 - rb=0.138, OR=0.55 (95% CI: 0.30-1.04, p=0.05-0.1); «AD+AR/ARC+BA» - rb=0.280, OR=0.15 (95% CI: 0.04-0.55, p<0.05). Conclusions. The homozygous genotype A/A SNV rs11466749 of TSLP gene significantly increases the risk of developing AM phenotypes «AR/ARC», «BA+AR/ARC» and «AD+AR/ARC+BA», and the heterozygous genotype A/G SNV rs11466749 of TSLP gene possesses a significantly protective effect on their development in children. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of
{"title":"Genotype-associated clinical markers of atopic phenotype development in children","authors":"V. Dytiatkovskyi","doi":"10.15574/pp.2023.93.45","DOIUrl":"https://doi.org/10.15574/pp.2023.93.45","url":null,"abstract":"Atopic march (AM) occurs as the linear progression of atopic disorders (AtD) from atopic dermatitis (AD) phenotype to its combinations with other AtD: allergic rhinitis/rhinoconjunctivitis (AR/ARC) and bronchial asthma (BA). Thymic stromal lymphopoietin (TSLP) plays an important role in AtD pathogenesis. Single nucleotide variant (SNV) rs11466749 of the TSLP gene during the last decades showed the controversial roles of A/A, A/G and G/G genotypes in occurrence of the mono- and polyorgan AM phenotypes in children. Purpose - to determine the associations and risks of AM phenotypes with homozygous or heterozygous SNV rs11466749 genotypes of the TSLP gene in children. Materials and methods. The study involved 398 children aged 3 to 18 years old: 293 children in the main group and 105 - in the control group. Patients of the main group suffered from AtD in 6 different AM phenotypes: «AD», «ARARC», «BA», «AD+AR/ARC», «BA+AR/ARC», «AD+AR/ARC+BA»; patients of the control group suffered from gastrointestinal pathology. Patients of all cohorts were genotyped for genotype variants A/A, A/G and G/G rs11466749 TSLP by the polymerase chain reaction in real time. Pearson’ contingency coefficient (rb), Pearson test (ꭕ2), Fisher’s exact test, odds ratio (OR) with a 95% confidence interval (95% CI) were used for statistical processing of the obtained results. Results at p≤0.05 were considered statistically significant. Results. The homozygous A/A rs11466749 TSLP genotype was significantly most frequent in phenotypes with mono- or oligoorgan affection «AR/ARC» and polyorgan affection «BA+AR/ARC» and «AD+AR/ARC+BA»: 66,2%, 65.3% and 76.9% respectively (p<0.05). Heterozygous genotype A/G rs11466749 TSLP was the second most significant and frequent: «AR/ARC» - 31.0% (p<0.05), «BA+AR/ARC» - 31.9% (p=0.05-0.1) and «AD+AR/ARC+BA» - 11.5% (p<0.05). Genotype A/A rs11466749 TSLP was significantly associated and increased the development risks of the 3 specified AM phenotypes: «AR/ARC» - rb=0.156, OR=1.92 (95% CI: 1.03-3.58, p<0.05); «BA+AR/ARC» - rb=0.147, OR=1.84 (95% CI: 1.0-3.42, p<0.05); «AD+AR/ARC+BA» - rb=0.212, OR=3.27 (95% CI: 1.22-8.80, p<0.05). Genotype A/G rs11466749 TSLP was reliably associated and had a protective effect on the development of bespoke AM phenotypes: «AR/ARC» - rb=0.148, OR=0.53 (95% CI: 0.28-1.0, p<0.05); «BA+AR/ARC2 - rb=0.138, OR=0.55 (95% CI: 0.30-1.04, p=0.05-0.1); «AD+AR/ARC+BA» - rb=0.280, OR=0.15 (95% CI: 0.04-0.55, p<0.05). Conclusions. The homozygous genotype A/A SNV rs11466749 of TSLP gene significantly increases the risk of developing AM phenotypes «AR/ARC», «BA+AR/ARC» and «AD+AR/ARC+BA», and the heterozygous genotype A/G SNV rs11466749 of TSLP gene possesses a significantly protective effect on their development in children. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of ","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116858968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pregnancy is a special state in a woman’s life, which can definitely be considered an «immunological paradox», because the growth of a genetically «alien» fetus takes place in a woman’s body. Despite the direct contact between cells of fetal origin (syncytiotrophoblast) and cells of the maternal immune system, which are in excess in the decidual membrane of the uterus, rejection of the semi-allogeneic fetus does not occur. A state of permanent natural immunological tolerance, during which the body does not respond to certain antigens expressed by trophoblast cells, while maintaining the ability to respond immunologically to other immunogens is the opposite phenomenon of the immune response, it is acquired by the body during its development and is not genetically determined. Purpose - to analyze the phasing of immunological changes in the mother’s body, which are aimed at the development and preservation of pregnancy, to specify their role in the correct flow of placental waves, prevention of the development of placental dysfunction and obstetric complications associated with it. A review of modern medical literature on the processes of immunological changes during trophoblast invasion and placentation in early pregnancy is given. For a more detailed understanding, the influence of each link of the immune response in the process of developing immune tolerance was analyzed. It has been established that for the development of a normal pregnancy there is a genetically programmed immune mechanism that ensures a decrease in the local and systemic immune response to the semi-alien implanted blastocyst, embryo and fetus. This is achieved through the step-by-step course of three phases of immunological shifts: the unfolded immune conflict; suppression of the immune response and intense immune conflict. The physiological course of gestation and the development of the placenta, in which the balance between the processes of neoangiogenesis and apoptosis is maintained, is ensured by adequate expression of HLA-G locus molecules by trophoblast cells, inhibition of Th1-type cytotoxic reactions against placenta cells by the mother's body. Analyzed changes in the cytokine balance, which shifts towards immunosuppressive cytokines, which suppress cellular immune reactions and stimulate the production of blocking antibodies, the quantitative composition of which can be considered decisive when carrying a genetically foreign fetus. Consequently, a relative secondary cellular immunodeficiency is formed, which is mainly due to a deficiency of circulating T-helper/inducers, a decrease in the CD4/CD8 immunoregulatory index and suppression of the functional activity of the T-cell immune system. It has been proven that impaired immunological tolerance, trophoblast invasion and vascular remodelling processes controlled by the maternal immune system at the local and systemic levels lead to early reproductive losses, premature birth, placental dysfunction, and are associated w
{"title":"Features of the functioning of immunological tolerance during pregnancy (literature review)","authors":"V. Panov, Y. Duka","doi":"10.15574/pp.2023.93.76","DOIUrl":"https://doi.org/10.15574/pp.2023.93.76","url":null,"abstract":"Pregnancy is a special state in a woman’s life, which can definitely be considered an «immunological paradox», because the growth of a genetically «alien» fetus takes place in a woman’s body. Despite the direct contact between cells of fetal origin (syncytiotrophoblast) and cells of the maternal immune system, which are in excess in the decidual membrane of the uterus, rejection of the semi-allogeneic fetus does not occur. A state of permanent natural immunological tolerance, during which the body does not respond to certain antigens expressed by trophoblast cells, while maintaining the ability to respond immunologically to other immunogens is the opposite phenomenon of the immune response, it is acquired by the body during its development and is not genetically determined. Purpose - to analyze the phasing of immunological changes in the mother’s body, which are aimed at the development and preservation of pregnancy, to specify their role in the correct flow of placental waves, prevention of the development of placental dysfunction and obstetric complications associated with it. A review of modern medical literature on the processes of immunological changes during trophoblast invasion and placentation in early pregnancy is given. For a more detailed understanding, the influence of each link of the immune response in the process of developing immune tolerance was analyzed. It has been established that for the development of a normal pregnancy there is a genetically programmed immune mechanism that ensures a decrease in the local and systemic immune response to the semi-alien implanted blastocyst, embryo and fetus. This is achieved through the step-by-step course of three phases of immunological shifts: the unfolded immune conflict; suppression of the immune response and intense immune conflict. The physiological course of gestation and the development of the placenta, in which the balance between the processes of neoangiogenesis and apoptosis is maintained, is ensured by adequate expression of HLA-G locus molecules by trophoblast cells, inhibition of Th1-type cytotoxic reactions against placenta cells by the mother's body. Analyzed changes in the cytokine balance, which shifts towards immunosuppressive cytokines, which suppress cellular immune reactions and stimulate the production of blocking antibodies, the quantitative composition of which can be considered decisive when carrying a genetically foreign fetus. Consequently, a relative secondary cellular immunodeficiency is formed, which is mainly due to a deficiency of circulating T-helper/inducers, a decrease in the CD4/CD8 immunoregulatory index and suppression of the functional activity of the T-cell immune system. It has been proven that impaired immunological tolerance, trophoblast invasion and vascular remodelling processes controlled by the maternal immune system at the local and systemic levels lead to early reproductive losses, premature birth, placental dysfunction, and are associated w","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115442355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Dudnyk, V. Furman, O. Kutsak, V. Pasik, O.P. Fedchyshen
Cystic fibrosis related diabetes (CFRD) is a rare pathology that combines genetically determined systemicity with damage to the exocrine glands, which leads to early manifestations of the disease, a clear exacerbation and chronicity of the process, with possible complications in bone remodeling with the occurrence of malignant tumors. Purpose - to acquaint practitioners with the peculiarities of manifestations, diagnosis and course of chondroblastic osteosarcoma in a child with CFRD. Features of the clinical course and differential diagnosis are described and given osteosarcoma of the proximal part of the left tibia against the background of CFRD in a child. The main diagnostic value of history, clinical picture, laboratory, instrumental, immunohistochemical and microscopic research methods was highlighted, in particular, a biopsy of this tumor. Microscopic examination of tissue biopsy revealed alternating areas of atypical cartilaginous tissue corresponding to the structure of chondrosarcoma 1-2 cm, solid proliferates of atypical fibroblast-like cells and areas of atypical and fibroblastic tissue containing elements of atypical osteogenesis. Periosteally - randomly oriented bone fragments at the place of the cortical plate, as well as impressions of the lower metaphysis of the thigh and the upper metaphysis of the tibia, near the knee joint. The immunohistochemical examination showed a clear differential diagnosis of the existing CD99-positive tumor cells (DAKO, clone 12E7). Some cells were positive for SATB2 (Cell Marque, clone EP281); tumor cells were negative for S-100 - SOX-10. The histological picture and immunophenotype of the tumor cells correspond to chondroblastic osteosarcoma. Osteosarcoma with genetic, metabolic and aplastic features developing in the setting of a comorbid background significantly complicates diagnosis and requires certain changes in the treatment tactics of CFRD (correction of prophylactic and basic therapy, metabolic and toxic disorders, including those caused by chemotherapy). The research was carried out in accordance with the principles of the Helsinki Declaration. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
{"title":"Chondroblastic osteosarcoma of the proximal left tibia in the setting of cystic fibrosis related diabetes mellitus. Clinical case","authors":"V. Dudnyk, V. Furman, O. Kutsak, V. Pasik, O.P. Fedchyshen","doi":"10.15574/pp.2023.93.123","DOIUrl":"https://doi.org/10.15574/pp.2023.93.123","url":null,"abstract":"Cystic fibrosis related diabetes (CFRD) is a rare pathology that combines genetically determined systemicity with damage to the exocrine glands, which leads to early manifestations of the disease, a clear exacerbation and chronicity of the process, with possible complications in bone remodeling with the occurrence of malignant tumors. Purpose - to acquaint practitioners with the peculiarities of manifestations, diagnosis and course of chondroblastic osteosarcoma in a child with CFRD. Features of the clinical course and differential diagnosis are described and given osteosarcoma of the proximal part of the left tibia against the background of CFRD in a child. The main diagnostic value of history, clinical picture, laboratory, instrumental, immunohistochemical and microscopic research methods was highlighted, in particular, a biopsy of this tumor. Microscopic examination of tissue biopsy revealed alternating areas of atypical cartilaginous tissue corresponding to the structure of chondrosarcoma 1-2 cm, solid proliferates of atypical fibroblast-like cells and areas of atypical and fibroblastic tissue containing elements of atypical osteogenesis. Periosteally - randomly oriented bone fragments at the place of the cortical plate, as well as impressions of the lower metaphysis of the thigh and the upper metaphysis of the tibia, near the knee joint. The immunohistochemical examination showed a clear differential diagnosis of the existing CD99-positive tumor cells (DAKO, clone 12E7). Some cells were positive for SATB2 (Cell Marque, clone EP281); tumor cells were negative for S-100 - SOX-10. The histological picture and immunophenotype of the tumor cells correspond to chondroblastic osteosarcoma. Osteosarcoma with genetic, metabolic and aplastic features developing in the setting of a comorbid background significantly complicates diagnosis and requires certain changes in the treatment tactics of CFRD (correction of prophylactic and basic therapy, metabolic and toxic disorders, including those caused by chemotherapy). The research was carried out in accordance with the principles of the Helsinki Declaration. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114807055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diseases of periodontal tissues, despite the developed treatment and prevention complexes, remain an urgent problem not only in modern dentistry but also in medicine in general. Periodontal tissue diseases occur as a result of a number of factors, both local and general. The pathogenesis of periodontal tissue diseases is complex and multilevel, with an important link being the increase in lipid peroxidation, weakening of the antioxidant defense system, as well as disruption of all metabolic processes characteristic of this disease under the influence of various factors, including smoking. Normally, the body’s lipid peroxidation system - the antioxidant defense system is in a state of dynamic equilibrium, functioning on the principle of feedback, supported by a certain organization of plasma and cellular lipids, a dynamic system of phospholipids and cholesterol (determining the lipid level of oxidation of cell membranes), and is a leading indicator of the body’s adaptive capabilities. Purpose - to study the biochemical parameters of oral fluid as markers for assessing the state of antioxidant and prooxidant systems in adolescent and young adult smokers. Materials and methods. The biochemical parameters of oral fluid were studied in 114 adolescents and young adults aged 15 to 24 years, who were divided into groups: the Group 1 included 26 people who regularly smoke traditional cigarettes; the Group 2 - 22 people who regularly smoke electronic cigarettes (vapes); the Group 3 - 23 people who regularly smoke tobacco heating devices (IQOS); the Group 4 - 43 people without a smoking habit. Biochemical parameters were studied: the activity of superoxide dismutase and catalase enzymes, dyne conjugants (DCs) and thiobarbituric acid-reactive products (TBA-RPs). Results. In patients of the Group 1, a decrease in the activity of superoxide dismutase in the oral fluid was found by 1.4 times compared with patients of the Group 4 (p<0.05); in patients of the Group 2 and the Group 3 - by 1.2 times, respectively (p<0.05). In patients of the Group 1, the enzyme activity was significantly reduced - by 2.6 times compared to patients of the Group 4 (p<0.05); in patients of the Group 2 and the Group 3 - by 1.8 times, respectively (p<0.05). In patients of the Group 1, an increase in the number of TBA-RPs was noted by 61% compared with patients of the Group 4; in patients of the Group 2 and the Group 3 - by 48% and 47%, respectively. In addition, the number of DCs in the oral fluid increased in the Group 1, as well as in the Group 2 and the Group 3 - by 29%, 17% and 15%, respectively, compared with the Group 4. Conclusions. In adolescent and young adult smokers, there is an increase in lipid peroxidation, which is determined by an increase in TBA-RPs and the level of DCs in the oral fluid, and a decrease in antioxidant properties, which is manifested by a decrease in the activity of superoxide dismutase and catalase enzymes, which can increase the destruction of membrane
{"title":"Biochemical indicators of oral fluid as markers for assessing the state of antioxidant-prooxidant systems in teenagers and young adults who smoke","authors":"I. Lisetska, M. Rozhko","doi":"10.15574/pp.2023.93.51","DOIUrl":"https://doi.org/10.15574/pp.2023.93.51","url":null,"abstract":"Diseases of periodontal tissues, despite the developed treatment and prevention complexes, remain an urgent problem not only in modern dentistry but also in medicine in general. Periodontal tissue diseases occur as a result of a number of factors, both local and general. The pathogenesis of periodontal tissue diseases is complex and multilevel, with an important link being the increase in lipid peroxidation, weakening of the antioxidant defense system, as well as disruption of all metabolic processes characteristic of this disease under the influence of various factors, including smoking. Normally, the body’s lipid peroxidation system - the antioxidant defense system is in a state of dynamic equilibrium, functioning on the principle of feedback, supported by a certain organization of plasma and cellular lipids, a dynamic system of phospholipids and cholesterol (determining the lipid level of oxidation of cell membranes), and is a leading indicator of the body’s adaptive capabilities. Purpose - to study the biochemical parameters of oral fluid as markers for assessing the state of antioxidant and prooxidant systems in adolescent and young adult smokers. Materials and methods. The biochemical parameters of oral fluid were studied in 114 adolescents and young adults aged 15 to 24 years, who were divided into groups: the Group 1 included 26 people who regularly smoke traditional cigarettes; the Group 2 - 22 people who regularly smoke electronic cigarettes (vapes); the Group 3 - 23 people who regularly smoke tobacco heating devices (IQOS); the Group 4 - 43 people without a smoking habit. Biochemical parameters were studied: the activity of superoxide dismutase and catalase enzymes, dyne conjugants (DCs) and thiobarbituric acid-reactive products (TBA-RPs). Results. In patients of the Group 1, a decrease in the activity of superoxide dismutase in the oral fluid was found by 1.4 times compared with patients of the Group 4 (p<0.05); in patients of the Group 2 and the Group 3 - by 1.2 times, respectively (p<0.05). In patients of the Group 1, the enzyme activity was significantly reduced - by 2.6 times compared to patients of the Group 4 (p<0.05); in patients of the Group 2 and the Group 3 - by 1.8 times, respectively (p<0.05). In patients of the Group 1, an increase in the number of TBA-RPs was noted by 61% compared with patients of the Group 4; in patients of the Group 2 and the Group 3 - by 48% and 47%, respectively. In addition, the number of DCs in the oral fluid increased in the Group 1, as well as in the Group 2 and the Group 3 - by 29%, 17% and 15%, respectively, compared with the Group 4. Conclusions. In adolescent and young adult smokers, there is an increase in lipid peroxidation, which is determined by an increase in TBA-RPs and the level of DCs in the oral fluid, and a decrease in antioxidant properties, which is manifested by a decrease in the activity of superoxide dismutase and catalase enzymes, which can increase the destruction of membrane","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126219445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Topicality. Up to 90% of patients with atopic dermatitis are colonized with S. aureus, with S. aureus predominance being unique to atopic dermatitis. TLR2 play a role in the presentation of S. aureus antigens in a course of atopic dermatits. Purpose - to investigate the association of rs4696480 polymorphism in TLR2 gene and S. aureus skin colonization. Materials and methods. The study included 101 patients with eczema and 84 healthy children. Skin swab cultures were taken. Subjects were classified as carriers if the cultures were positive, while those with culture found to be negative were classified as non-carriers. Genotyping for TLR2 rs4696480 was performed by using Real-time PCR. Results. We determined the prevalence of S. aureus carriage in a cohort study of atopic dermatitis patients in Ukraine. Skin culture for the presence of S. aureus was performed in 82 patients: 45.1% children had positive culture for S. aureus, 54.9% had a negative result. SCORing Atopic Dermatitis (SCORAD) index was significantly higher in S. aureus carriers (p<0.001). There was no difference in genotype distribution among patients and control group (OR=1.021 (95% CI 0.507-2.054) for AT genotype, OR=0.880 (95% CI 0.398-1.947) for TT genotype, р>0.05). AA genotype was significantly more frequent among S. aureus carriers (OR=2.745 (95% CI 0.865-8.708) for AT genotype, OR=7.000 (95% CI 1.852-26.462) for TT genotype. To our knowledge, the association of T16934A (rs4696480) and S. aureus colonization of lesion skin in children with atopic dermatitis have not been studied before. Conclusions. AA variant of TLR2 rs4696480 polymorphism predisposes to S. aureus colonization of skin in children with atopic dermatits. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the author.
时事性。高达90%的特应性皮炎患者有金黄色葡萄球菌定植，金黄色葡萄球菌优势是特应性皮炎所特有的。TLR2在特应性皮炎病程中参与金黄色葡萄球菌抗原的呈递。目的:探讨TLR2基因rs4696480多态性与金黄色葡萄球菌皮肤定植的关系。材料和方法。该研究包括101名湿疹患者和84名健康儿童。进行皮肤拭子培养。如果培养结果为阳性，受试者被归类为携带者，而那些培养结果为阴性的受试者被归类为非携带者。采用Real-time PCR对TLR2 rs4696480进行基因分型。结果。我们在乌克兰特应性皮炎患者的队列研究中确定了金黄色葡萄球菌携带的患病率。对82例患者进行皮肤金黄色葡萄球菌培养:45.1%的儿童金黄色葡萄球菌培养阳性，54.9%的儿童金黄色葡萄球菌培养阴性。金黄色葡萄球菌携带者的特应性皮炎(SCORAD)指数显著高于对照组(p0.05)。AA基因型在金黄色葡萄球菌携带者中更为常见(AT基因型OR=2.745 (95% CI 0.865 ~ 8.708)， TT基因型OR=7.000 (95% CI 1.852 ~ 26.462)。据我们所知，T16934A (rs4696480)与金黄色葡萄球菌在特应性皮炎患儿病变皮肤中的定植之间的关系尚未有研究。结论。TLR2 rs4696480多态性AA变异体易致金黄色葡萄球菌在特应性皮炎患儿皮肤定植这项研究是按照《赫尔辛基宣言》的原则进行的。研究方案经参与机构当地伦理委员会批准。获得患者的知情同意进行研究。作者未声明存在利益冲突。
{"title":"The association of rs4696480 polymorphism in TLR2 gene and Staphylococcus aureus skin colonization in children with atopic dermatitis","authors":"O. Mozyrska","doi":"10.15574/pp.2022.92.28","DOIUrl":"https://doi.org/10.15574/pp.2022.92.28","url":null,"abstract":"Topicality. Up to 90% of patients with atopic dermatitis are colonized with S. aureus, with S. aureus predominance being unique to atopic dermatitis. TLR2 play a role in the presentation of S. aureus antigens in a course of atopic dermatits. Purpose - to investigate the association of rs4696480 polymorphism in TLR2 gene and S. aureus skin colonization. Materials and methods. The study included 101 patients with eczema and 84 healthy children. Skin swab cultures were taken. Subjects were classified as carriers if the cultures were positive, while those with culture found to be negative were classified as non-carriers. Genotyping for TLR2 rs4696480 was performed by using Real-time PCR. Results. We determined the prevalence of S. aureus carriage in a cohort study of atopic dermatitis patients in Ukraine. Skin culture for the presence of S. aureus was performed in 82 patients: 45.1% children had positive culture for S. aureus, 54.9% had a negative result. SCORing Atopic Dermatitis (SCORAD) index was significantly higher in S. aureus carriers (p<0.001). There was no difference in genotype distribution among patients and control group (OR=1.021 (95% CI 0.507-2.054) for AT genotype, OR=0.880 (95% CI 0.398-1.947) for TT genotype, р>0.05). AA genotype was significantly more frequent among S. aureus carriers (OR=2.745 (95% CI 0.865-8.708) for AT genotype, OR=7.000 (95% CI 1.852-26.462) for TT genotype. To our knowledge, the association of T16934A (rs4696480) and S. aureus colonization of lesion skin in children with atopic dermatitis have not been studied before. Conclusions. AA variant of TLR2 rs4696480 polymorphism predisposes to S. aureus colonization of skin in children with atopic dermatits. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the author.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115804915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose - tо determine the optimized system of preoperative preparation of patients with arterial (AH) and pulmonary hypertension (PH). Materials and methods. During the work, 35 patients were examined, 25 (71.4%) hypertensive patients were prepared for surgical treatment, 19 (54.3%) of them underwent planned operations, 6 (17.1%) underwent surgical urgent intervention. 10 (28.6%) patients with PH were examined and prepared for surgery, in which surgical intervention was performed as planned. The patients who underwent planned operations were examined according to the standards of the medical institution, taking into account protocols of the European Association of Cardiologists (ESC) from 2022. Results. Stage I AH was diagnosed in 15 (42.9%) patients, and stage II - in 10 (28.6%) women. Stage I PH was detected in 7 (20%) patients, and stage II - in 3 (8.6%) women. When preparing patients for surgery, drug therapy of AH was carefully selected, which affected different links of the pathogenesis of the disease. In patients with PH, who are scheduled for surgical treatment, drug therapy in many cases is clearly regulated and prescribed in accordance with the clinical manifestations of the disease and the course of the perioperative period. No cardiovascular complications were detected in patients who were operated on as planned in compliance with the developed scheme of preoperative preparation. Blood pressure in most cases was within the age norm, there were no cases of venous thromboembolism, the severity of heart failure did not increase. Conclusions. It is recommended to strictly follow the scheme of preoperative preparation, starting from the prehospital stage of examination and treatment of patients with AH and PH. Determination and adherence to the optimized scheme of preoperative preparation proved to be effective, contributed to the absence of complications from the cardiovascular system, stabilization of blood pressure in the perioperative period, was characterized by the absence of cases of venous thromboembolism, and the severity of heart failure did not increase. The study was performed in accordance with the principles of the Declaration of Helsinki. The study protocol was approved by the Local Ethics Committee of the institution. Informed consent of patients was obtained for the study. No conflict of interests was declared by the authors.
{"title":"Preoperative management of patients with arterial and pulmonary hypertension combined with gynecological and surgical diseases","authors":"V. Dronova, O. Dronov, R. Teslyuk, O. Mokryk","doi":"10.15574/pp.2022.92.5","DOIUrl":"https://doi.org/10.15574/pp.2022.92.5","url":null,"abstract":"Purpose - tо determine the optimized system of preoperative preparation of patients with arterial (AH) and pulmonary hypertension (PH). Materials and methods. During the work, 35 patients were examined, 25 (71.4%) hypertensive patients were prepared for surgical treatment, 19 (54.3%) of them underwent planned operations, 6 (17.1%) underwent surgical urgent intervention. 10 (28.6%) patients with PH were examined and prepared for surgery, in which surgical intervention was performed as planned. The patients who underwent planned operations were examined according to the standards of the medical institution, taking into account protocols of the European Association of Cardiologists (ESC) from 2022. Results. Stage I AH was diagnosed in 15 (42.9%) patients, and stage II - in 10 (28.6%) women. Stage I PH was detected in 7 (20%) patients, and stage II - in 3 (8.6%) women. When preparing patients for surgery, drug therapy of AH was carefully selected, which affected different links of the pathogenesis of the disease. In patients with PH, who are scheduled for surgical treatment, drug therapy in many cases is clearly regulated and prescribed in accordance with the clinical manifestations of the disease and the course of the perioperative period. No cardiovascular complications were detected in patients who were operated on as planned in compliance with the developed scheme of preoperative preparation. Blood pressure in most cases was within the age norm, there were no cases of venous thromboembolism, the severity of heart failure did not increase. Conclusions. It is recommended to strictly follow the scheme of preoperative preparation, starting from the prehospital stage of examination and treatment of patients with AH and PH. Determination and adherence to the optimized scheme of preoperative preparation proved to be effective, contributed to the absence of complications from the cardiovascular system, stabilization of blood pressure in the perioperative period, was characterized by the absence of cases of venous thromboembolism, and the severity of heart failure did not increase. The study was performed in accordance with the principles of the Declaration of Helsinki. The study protocol was approved by the Local Ethics Committee of the institution. Informed consent of patients was obtained for the study. No conflict of interests was declared by the authors.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131389761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose - is to study the effect of smoking on the biochemical indicators of oral fluid in teenagers and young adults. Materials and methods. The study of biochemical indicators of oral fluid was carried out of 114 teenagers and young adults from 15 to 24 years’ old who were divided into groups: the Group I included 26 people who regularly smoke traditional cigarettes; the Group II - 22 people who regularly smoke electronic cigarettes (Vapes); the Group III - 23 people who regularly smoke devices for heating tobacco (IQOS); the Group IV - 43 people without the harmful habit of smoking. Biochemical indicators were studied: the activity of alkaline and acid phosphatase, the content of inorganic phosphorus and calcium. Results. The average values of alkaline phosphatase activity in the examined subjects of the I group were 1.5 times lower compared to persons of teenagers and young adults who do not smoke (IV group), (p<0.05). Alkaline phosphatase activity was also 1.2 times lower in people who smoke alternative types of cigarettes compared to non-smokers (p<0.05). At the same time, an increased activity of acid phosphatase was found, the indicators of which also depended on the type of smoking. Thus, the activity of acid phosphatase was 3.6 times higher in the examined subjects of the I group compared to persons of teenagers and young adults who do not smoke (IV group), (p<0.05). In people who smoke alternative types of cigarettes, the activity of acid phosphatase was 2.2 times higher compared to people who do not smoke (p<0.05). In the oral fluid of teenagers and young adults who have a bad habit, an increased content of calcium and a reduced content of inorganic phosphorus were found compared to those examined in the IV group. Conclusions. The obtained data indicate a violation of the normal functional activity of the organs of the oral cavity under the influence of a bad habit. Changes in the biochemical parameters of the oral fluid occur in adolescents and young adults who smoke. Oral fluid parameters may be an early prognostic test for assessing oral health in teenagers and young adults who smoke. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
{"title":"The influence of smoking on the biochemical indicators of oral fluid in teenagers and young adults who smoke","authors":"I. Lisetska","doi":"10.15574/pp.2022.92.37","DOIUrl":"https://doi.org/10.15574/pp.2022.92.37","url":null,"abstract":"Purpose - is to study the effect of smoking on the biochemical indicators of oral fluid in teenagers and young adults. Materials and methods. The study of biochemical indicators of oral fluid was carried out of 114 teenagers and young adults from 15 to 24 years’ old who were divided into groups: the Group I included 26 people who regularly smoke traditional cigarettes; the Group II - 22 people who regularly smoke electronic cigarettes (Vapes); the Group III - 23 people who regularly smoke devices for heating tobacco (IQOS); the Group IV - 43 people without the harmful habit of smoking. Biochemical indicators were studied: the activity of alkaline and acid phosphatase, the content of inorganic phosphorus and calcium. Results. The average values of alkaline phosphatase activity in the examined subjects of the I group were 1.5 times lower compared to persons of teenagers and young adults who do not smoke (IV group), (p<0.05). Alkaline phosphatase activity was also 1.2 times lower in people who smoke alternative types of cigarettes compared to non-smokers (p<0.05). At the same time, an increased activity of acid phosphatase was found, the indicators of which also depended on the type of smoking. Thus, the activity of acid phosphatase was 3.6 times higher in the examined subjects of the I group compared to persons of teenagers and young adults who do not smoke (IV group), (p<0.05). In people who smoke alternative types of cigarettes, the activity of acid phosphatase was 2.2 times higher compared to people who do not smoke (p<0.05). In the oral fluid of teenagers and young adults who have a bad habit, an increased content of calcium and a reduced content of inorganic phosphorus were found compared to those examined in the IV group. Conclusions. The obtained data indicate a violation of the normal functional activity of the organs of the oral cavity under the influence of a bad habit. Changes in the biochemical parameters of the oral fluid occur in adolescents and young adults who smoke. Oral fluid parameters may be an early prognostic test for assessing oral health in teenagers and young adults who smoke. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115569323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Dudnyk, N.O. Buhlova, I. Morozova, T.Y. Kukuruza
Background. Digestive tract diseases occupy one of the first places in the structure of childhood pathology, including duodenal ulcers (DU), the development of which in 70-100% of cases is associated with H.pylori infection. It is found that the important role in the development of inflammatory process in digestive tract have toll-like receptors 4 (TLR4), due to reactiveness to H.pylori. It is proved that growth factors, in particular epidermal growth factor (EGF), are able to influence on the healing processes in the mucous membrane. It is advisable to include drugs of reparative action in complex treatment of pediatric DU. Purpose - to evaluate the effect of the reparative action drug in the treatment of pediatric DU, taking into account the dynamics of TLR4 and EGF in blood serum. Materials and methods. The total clinical group consisted of 39 children aged 7-18 years with H.pylori-associated duodenal ulcer. In 20 patients of the group 1 with DU a standard triple regimen was used. In 19 patients of the group 2 a drug of reparative action was included. The content of TLR4 and EGF in serum were determined by ELISA. Results. It was found that the level of TLR 4 decreased by 15.1% and 27.5% after a course of treatment in patients of the group 1 and in the group 2, respectively сompared to a level before treatment. It is showed that in patients of the group 1 and the group 2 after 3 weeks from the start of treatment there were changes in the content of EGF manifested by a decrease by 19.81% and 23.3%, сompared to a level before treatment. Сonclusions. Our results indicated that after the course of treatment the content of TLR4 and EGF in the group 1 of patients was lower by 12.37% and 3.49% respectively, than in the group 2. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
{"title":"The effect of the reparative action drug in treatment of pediatric duodenal ulcer","authors":"V. Dudnyk, N.O. Buhlova, I. Morozova, T.Y. Kukuruza","doi":"10.15574/pp.2022.92.33","DOIUrl":"https://doi.org/10.15574/pp.2022.92.33","url":null,"abstract":"Background. Digestive tract diseases occupy one of the first places in the structure of childhood pathology, including duodenal ulcers (DU), the development of which in 70-100% of cases is associated with H.pylori infection. It is found that the important role in the development of inflammatory process in digestive tract have toll-like receptors 4 (TLR4), due to reactiveness to H.pylori. It is proved that growth factors, in particular epidermal growth factor (EGF), are able to influence on the healing processes in the mucous membrane. It is advisable to include drugs of reparative action in complex treatment of pediatric DU. Purpose - to evaluate the effect of the reparative action drug in the treatment of pediatric DU, taking into account the dynamics of TLR4 and EGF in blood serum. Materials and methods. The total clinical group consisted of 39 children aged 7-18 years with H.pylori-associated duodenal ulcer. In 20 patients of the group 1 with DU a standard triple regimen was used. In 19 patients of the group 2 a drug of reparative action was included. The content of TLR4 and EGF in serum were determined by ELISA. Results. It was found that the level of TLR 4 decreased by 15.1% and 27.5% after a course of treatment in patients of the group 1 and in the group 2, respectively сompared to a level before treatment. It is showed that in patients of the group 1 and the group 2 after 3 weeks from the start of treatment there were changes in the content of EGF manifested by a decrease by 19.81% and 23.3%, сompared to a level before treatment. Сonclusions. Our results indicated that after the course of treatment the content of TLR4 and EGF in the group 1 of patients was lower by 12.37% and 3.49% respectively, than in the group 2. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128512843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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