Following the biopsychosocial model of medicine, irritable bowel syndrome (IBS) is a heterogeneous disorder, which is caused by multiple factors in different combinations. However, in most clinical cases probiotics are included in the treatment of IBS due to their influence on intestinal bacterial colonization, and immune, metabolic and motoric activity of the gut. The purpose - to evaluate the efficiency of probiotics (Lactobacillus reuteri DSM 17938) in children with IBS and to determine the optimal duration of the treatment. Materials and methods. We examined 108 children aged 6-12 years with a verified diagnosis of IBS, according to Rome criteria IV. Assessment of the main clinical symptoms was obtained by 4-point Likert scale. Enzyme immunoassay RIDASCREEN Calprotectin (R-Biopharm AG, Germany) was used for the quantitative determination of calprotectin in stool samples. Data were processed using Microsoft Excel 2016 and analysed with GraphPad (Prism 5.0). Results. Our study revealed the heterogenety of IBS in children not only according to clinical subtype, but also due to the trigger factor of the disorder. Patients with stress-related IBS were characterized by the higher level of asthenic syndrome and autonomic dysfunction (р=0.0003). In contrast, children with post-infectious IBS had higher concentration of fecal calprotectin, which is a result of low-grade intestinal inflammation (р=0.0003). After 10 days and 1 month since the beginning of treatment, we have observed a significant decrease in the severity of clinical syndromes, but the level of fecal calprotectin remained elevated. Conclusions. The efficiency of probiotics was confirmed by positive dynamics of clinical signs and the level of fecal calprotectin in children with various clinical subtypes of IBS. Even in the absence of any symptoms, the optimal duration of the use of Lactobacillus reuteri DSM 17938 in children with IBS should be 1-3 months. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of all participating institutions. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
{"title":"Efficiency of probiotics in children with irritable bowel syndrome: optimal duration of the treatment","authors":"M. Semen, O. Lychkovska, V. Şemen, A.J. Malakhova","doi":"10.15574/pp.2022.91.41","DOIUrl":"https://doi.org/10.15574/pp.2022.91.41","url":null,"abstract":"Following the biopsychosocial model of medicine, irritable bowel syndrome (IBS) is a heterogeneous disorder, which is caused by multiple factors in different combinations. However, in most clinical cases probiotics are included in the treatment of IBS due to their influence on intestinal bacterial colonization, and immune, metabolic and motoric activity of the gut. The purpose - to evaluate the efficiency of probiotics (Lactobacillus reuteri DSM 17938) in children with IBS and to determine the optimal duration of the treatment. Materials and methods. We examined 108 children aged 6-12 years with a verified diagnosis of IBS, according to Rome criteria IV. Assessment of the main clinical symptoms was obtained by 4-point Likert scale. Enzyme immunoassay RIDASCREEN Calprotectin (R-Biopharm AG, Germany) was used for the quantitative determination of calprotectin in stool samples. Data were processed using Microsoft Excel 2016 and analysed with GraphPad (Prism 5.0). Results. Our study revealed the heterogenety of IBS in children not only according to clinical subtype, but also due to the trigger factor of the disorder. Patients with stress-related IBS were characterized by the higher level of asthenic syndrome and autonomic dysfunction (р=0.0003). In contrast, children with post-infectious IBS had higher concentration of fecal calprotectin, which is a result of low-grade intestinal inflammation (р=0.0003). After 10 days and 1 month since the beginning of treatment, we have observed a significant decrease in the severity of clinical syndromes, but the level of fecal calprotectin remained elevated. Conclusions. The efficiency of probiotics was confirmed by positive dynamics of clinical signs and the level of fecal calprotectin in children with various clinical subtypes of IBS. Even in the absence of any symptoms, the optimal duration of the use of Lactobacillus reuteri DSM 17938 in children with IBS should be 1-3 months. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of all participating institutions. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134600020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Dynnik, O. Dynnik, S. Novokhatska, A.E. Druzhinina
The purpose - to identify significant prognostic criteria for the development of a complicated course of oligomenorrhea. Materials and methods. A clinical and hormonal examination of 64 adolescent girls with oligomenorrhea aged 12-18 was carried out. The following were determined: physical with determination of body mass index, sexual development, parameters of the hormonal background, vitamin D status, lipid spectrum and the state of carbohydrate metabolism. Results. The most informative sings regarding the formation of metabolic complications of the course of oligomenorrhea were selected by determining the informative clinical and anamnestic signs, hormonal indicators and the status of vitamin D. The following clinical parameters, which may indicate a complicated course of oligomenorrhea with disturbances in carbohydrate metabolism, had the greatest overall informativeness: macrosomia at birth; the mother’s age is more than 35 years at the time of our patient’s birth; in a girl, the age of menarche is up to 11 years, the presence of comorbid pathology (especially cardiovascular disorders) and body weight deficiency. The greatest prognostic significance from the hormonal parameters had high levels of luteinizing hormone, testosterone, total cholesterol, and low estradiol. The clinical, anamnestic and hormonal indicators that may indicate the formation of complications from lipid spectrum disorders were found. It was: deviation of the body weight at birth in both sides, high serum levels of prolactin, cortisol, normative follicle-stimulating hormone, and a moderate decrease in the level of vitamin D. Conclusions. Significant risk factors for the formation of metabolic complications in girls with oligomenorrhea have been identified. Using them makes it possible to timely determine the prognosis of the course of oligomenorrhea in the majority of patients already at the early stages of the formation of this pathology, to decide on therapy and the implementation of therapeutic and preventive measures in the future. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the SI «Institute of Health Care of Children and Adolescents of the NAMS of Ukraine». The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
{"title":"Risk factors for the development of complicated oligomenorrhea in adolescent girls","authors":"V. Dynnik, O. Dynnik, S. Novokhatska, A.E. Druzhinina","doi":"10.15574/pp.2022.91.29","DOIUrl":"https://doi.org/10.15574/pp.2022.91.29","url":null,"abstract":"The purpose - to identify significant prognostic criteria for the development of a complicated course of oligomenorrhea. Materials and methods. A clinical and hormonal examination of 64 adolescent girls with oligomenorrhea aged 12-18 was carried out. The following were determined: physical with determination of body mass index, sexual development, parameters of the hormonal background, vitamin D status, lipid spectrum and the state of carbohydrate metabolism. Results. The most informative sings regarding the formation of metabolic complications of the course of oligomenorrhea were selected by determining the informative clinical and anamnestic signs, hormonal indicators and the status of vitamin D. The following clinical parameters, which may indicate a complicated course of oligomenorrhea with disturbances in carbohydrate metabolism, had the greatest overall informativeness: macrosomia at birth; the mother’s age is more than 35 years at the time of our patient’s birth; in a girl, the age of menarche is up to 11 years, the presence of comorbid pathology (especially cardiovascular disorders) and body weight deficiency. The greatest prognostic significance from the hormonal parameters had high levels of luteinizing hormone, testosterone, total cholesterol, and low estradiol. The clinical, anamnestic and hormonal indicators that may indicate the formation of complications from lipid spectrum disorders were found. It was: deviation of the body weight at birth in both sides, high serum levels of prolactin, cortisol, normative follicle-stimulating hormone, and a moderate decrease in the level of vitamin D. Conclusions. Significant risk factors for the formation of metabolic complications in girls with oligomenorrhea have been identified. Using them makes it possible to timely determine the prognosis of the course of oligomenorrhea in the majority of patients already at the early stages of the formation of this pathology, to decide on therapy and the implementation of therapeutic and preventive measures in the future. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the SI «Institute of Health Care of Children and Adolescents of the NAMS of Ukraine». The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129897347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Smoking remains one of the serious medical and social problems of today. According to the information center on the problems of alcohol, smoking and drugs in Ukraine, 19 million people aged 15 and older smoke cigarettes (34% of them smoke daily, 6% smoke occasionally), which is the highest indicator among European countries. Smokers develop addiction, which can be physical, psychological and social in nature. The purpose - to determine the prevalence of bad habits, the degree of physical and psychological dependence among teenagers and young adults. Materials and methods. To achieve the goal, a survey was conducted using a specially developed questionnaire of 136 teenagers and young adults aged 15 to 24. To assess the degree of physical and psychological dependence, the Fageström test was conducted, the motivation to quit smoking, and the determination of the type of behavior of a smoker. Results of the study showed that this bad habit occurs both among teenagers and among respondents of young age. Among all respondents, 32.4±1.5% declared themselves to be daily smokers. Among the surveyed smokers, 17.6% of respondents had very weak nicotine addiction, 39.1% had low, 23.5% had moderate, 16.2% had high, and 3.6% had very high. When evaluating the types of smoker’s behavior, it was established that the leading type among respondents is “support” - found in 42.8%; the second place is occupied by the “relaxation” type - in 22.5%; in the third place in terms of frequency of detection is the type “playing with a cigarette”, detected in 20.5%. Respondents have average motivation to quit smoking - 52.1%, high motivation - 36.2% and low motivation - 11.7%. Conclusions. The conducted research showed that the problem of smoking among teenagers and young adults is relevant. The obtained results of the study indicate the need to develop individual programs for this contingent, regularly conduct motivational talks, as well as the simultaneous use of several different measures to combat tobacco smoking. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
{"title":"Assessment of the degree of psychological and physical dependence among teenagers and young adults who smoke","authors":"I. Lisetska, M. Rozhko","doi":"10.15574/pp.2022.91.48","DOIUrl":"https://doi.org/10.15574/pp.2022.91.48","url":null,"abstract":"Smoking remains one of the serious medical and social problems of today. According to the information center on the problems of alcohol, smoking and drugs in Ukraine, 19 million people aged 15 and older smoke cigarettes (34% of them smoke daily, 6% smoke occasionally), which is the highest indicator among European countries. Smokers develop addiction, which can be physical, psychological and social in nature. The purpose - to determine the prevalence of bad habits, the degree of physical and psychological dependence among teenagers and young adults. Materials and methods. To achieve the goal, a survey was conducted using a specially developed questionnaire of 136 teenagers and young adults aged 15 to 24. To assess the degree of physical and psychological dependence, the Fageström test was conducted, the motivation to quit smoking, and the determination of the type of behavior of a smoker. Results of the study showed that this bad habit occurs both among teenagers and among respondents of young age. Among all respondents, 32.4±1.5% declared themselves to be daily smokers. Among the surveyed smokers, 17.6% of respondents had very weak nicotine addiction, 39.1% had low, 23.5% had moderate, 16.2% had high, and 3.6% had very high. When evaluating the types of smoker’s behavior, it was established that the leading type among respondents is “support” - found in 42.8%; the second place is occupied by the “relaxation” type - in 22.5%; in the third place in terms of frequency of detection is the type “playing with a cigarette”, detected in 20.5%. Respondents have average motivation to quit smoking - 52.1%, high motivation - 36.2% and low motivation - 11.7%. Conclusions. The conducted research showed that the problem of smoking among teenagers and young adults is relevant. The obtained results of the study indicate the need to develop individual programs for this contingent, regularly conduct motivational talks, as well as the simultaneous use of several different measures to combat tobacco smoking. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125335696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose - to analyze modern approaches to understanding the mechanisms of occurrence and etiological factors of acute urticaria in children. Acute urticaria remains a common pathology in the structure of emergency conditions. That is why there is a need for a unified approach to understanding the mechanisms of its occurrence, as well as the management of such patients. The results of modern research on the interpretation of the causes of the appearance of acute urticaria in childhood are given. Identification of an etiological factor is not always successful; triggers or risk factors can be confirmed only in 21-55% of cases. However, this fact does not diminish the interest in the problem. Taking into account the fact that the mechanisms of urticaria can be immune or non-immune, their clinical features are given, detailing possible etiological factors. Considerable attention is paid to the mechanisms of mast cell degranulation, which can be caused by immune effects (IgE, activation of the complement system, immune complexes); direct influence of certain chemical or physical factors on fat cells without the development of a hypersensitivity reaction (histamine release); or instability of mast cell membranes (congenital defects). The authors highlight good reasons among medicines and food products that have a pronounced histamine-releasing ability, which helps the practicing doctor in understanding the mechanisms of urticaria. Given the children’s age, many researchers are interested in the role of infectious agents in the pathogenesis of urticaria. It is generally known about the high prevalence of acute infectious pathology in children, as well as the higher infectious index especially in children of the first years of life. According to the results of numerous publications, an obvious connection with viral and bacterial infections has been established in more than 80% of children with acute urticaria. It is often difficult for the clinician that acute viral infections can cause rashes on the skin, which must be differentiated from urticaria. It is appropriate to recall that clinically, urticaria can be manifested by a large number of diseases: somatic, oncological, autoimmune, which requires careful differentiation. No conflict of interests was declared by the authors.
{"title":"Modern views on etiopathogenetic mechanisms of acute urticaria in children","authors":"N. Banadyha, A. Nakonechna","doi":"10.15574/pp.2022.91.54","DOIUrl":"https://doi.org/10.15574/pp.2022.91.54","url":null,"abstract":"The purpose - to analyze modern approaches to understanding the mechanisms of occurrence and etiological factors of acute urticaria in children. Acute urticaria remains a common pathology in the structure of emergency conditions. That is why there is a need for a unified approach to understanding the mechanisms of its occurrence, as well as the management of such patients. The results of modern research on the interpretation of the causes of the appearance of acute urticaria in childhood are given. Identification of an etiological factor is not always successful; triggers or risk factors can be confirmed only in 21-55% of cases. However, this fact does not diminish the interest in the problem. Taking into account the fact that the mechanisms of urticaria can be immune or non-immune, their clinical features are given, detailing possible etiological factors. Considerable attention is paid to the mechanisms of mast cell degranulation, which can be caused by immune effects (IgE, activation of the complement system, immune complexes); direct influence of certain chemical or physical factors on fat cells without the development of a hypersensitivity reaction (histamine release); or instability of mast cell membranes (congenital defects). The authors highlight good reasons among medicines and food products that have a pronounced histamine-releasing ability, which helps the practicing doctor in understanding the mechanisms of urticaria. Given the children’s age, many researchers are interested in the role of infectious agents in the pathogenesis of urticaria. It is generally known about the high prevalence of acute infectious pathology in children, as well as the higher infectious index especially in children of the first years of life. According to the results of numerous publications, an obvious connection with viral and bacterial infections has been established in more than 80% of children with acute urticaria. It is often difficult for the clinician that acute viral infections can cause rashes on the skin, which must be differentiated from urticaria. It is appropriate to recall that clinically, urticaria can be manifested by a large number of diseases: somatic, oncological, autoimmune, which requires careful differentiation. No conflict of interests was declared by the authors.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124569710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose - to study the level of the serum fatty acids in children with autism spectrum disorders (ASD); to compare omega-6/omega-3 ratio depending of the clinical manifestation epileptic encephalopathy (EE) and ASD and electroencephalography data (EEG). Materials and methods. The study examined 101 children aged 2-6 years (mean age 4.02±0.95 years) with clinical manifestations ASD, who had been treated in a neuropsychiatric unit of the SI «Institute of Pediatrics, Obstetrics and Gynecology named after academician O.M. Lukyanova of the NAMS of Ukraine». According to the clinical features of epileptic encephalopathies and ASD, EEG monitoring data formed three groups: the group 1 - 35 children with clinical manifestations of ASD but without epileptic seizures and epileptiform changes in the EEG; the group 2 - 32 children with cognitive disintegration, clinical manifestations of ASD in which parents and health professionals did not note or noted a single history of epileptic seizures, but with epileptiform changes in the EEG; the group 3 - 34 children with clinical manifestations of ASD in which parents or health professionals have noted epileptic seizures and epileptiform changes in the EEG. In all children the level of blood fatty acids was determined by gas-liquid chromatography. Results. Indicators of fatty acid concentration indicate that the serum of children showed a significantly low level of omega-3 polyunsaturated fatty acids (PUFA) and saturation of omega-6 po PUFA in all groups of children. The level of concentration of the main metabolites of essential fatty acids reflects the imbalance due to deficiency of essential linolenic acid and its metabolite - docosapentaenoic acid, especially in children with ASD and epileptiform changes in the EEG (group 2), (p<0.01). Among the omega-6 PUFA found a high content of essential linoleic acid and its metabolite - arachidonic acid, which causes the total excess of this group of PUFA, more pronounced in the groups 1 and 2. The level of index of unsaturated fatty acids in children from the group 1 was 10.1, in children from the group 2 - 9.8, and in the group 3 - 9.3. The obtained results of the assessment of the unsaturation index reflect the lower level of index of unsaturated fatty acids in children of the group 3 who have manifestations of ASD and epileptic seizures compared to other groups (p<0.05, r=0.99). Conclusions. The data is a reliable evidence that children with ASD, epileptic seizures and epileptiform changes in the EEG have higher levels of omega-6 PUFA and arachidonic acid and omega-3 and docosahexaenoic acid deficiency. The most pronounced imbalance in both the ratio of omega-6 / omega-3 and arachidonic acid / docosahexaenoic acid in the group of children with ASD and epileptiform changes in the EEG. These results may support the theory of changes in the properties of the phospholipid membrane and ion channels of nerve cells and require further study using electron microscopy. The res
{"title":"Features of omega-3/omega-6 fatty acid metabolism in children with epileptic encephalopathies and autism spectrum disorders","authors":"L. Kyrylova, О.О. Yuzva, O. Miroshnikov","doi":"10.15574/pp.2022.90.31","DOIUrl":"https://doi.org/10.15574/pp.2022.90.31","url":null,"abstract":"Purpose - to study the level of the serum fatty acids in children with autism spectrum disorders (ASD); to compare omega-6/omega-3 ratio depending of the clinical manifestation epileptic encephalopathy (EE) and ASD and electroencephalography data (EEG). Materials and methods. The study examined 101 children aged 2-6 years (mean age 4.02±0.95 years) with clinical manifestations ASD, who had been treated in a neuropsychiatric unit of the SI «Institute of Pediatrics, Obstetrics and Gynecology named after academician O.M. Lukyanova of the NAMS of Ukraine». According to the clinical features of epileptic encephalopathies and ASD, EEG monitoring data formed three groups: the group 1 - 35 children with clinical manifestations of ASD but without epileptic seizures and epileptiform changes in the EEG; the group 2 - 32 children with cognitive disintegration, clinical manifestations of ASD in which parents and health professionals did not note or noted a single history of epileptic seizures, but with epileptiform changes in the EEG; the group 3 - 34 children with clinical manifestations of ASD in which parents or health professionals have noted epileptic seizures and epileptiform changes in the EEG. In all children the level of blood fatty acids was determined by gas-liquid chromatography. Results. Indicators of fatty acid concentration indicate that the serum of children showed a significantly low level of omega-3 polyunsaturated fatty acids (PUFA) and saturation of omega-6 po PUFA in all groups of children. The level of concentration of the main metabolites of essential fatty acids reflects the imbalance due to deficiency of essential linolenic acid and its metabolite - docosapentaenoic acid, especially in children with ASD and epileptiform changes in the EEG (group 2), (p<0.01). Among the omega-6 PUFA found a high content of essential linoleic acid and its metabolite - arachidonic acid, which causes the total excess of this group of PUFA, more pronounced in the groups 1 and 2. The level of index of unsaturated fatty acids in children from the group 1 was 10.1, in children from the group 2 - 9.8, and in the group 3 - 9.3. The obtained results of the assessment of the unsaturation index reflect the lower level of index of unsaturated fatty acids in children of the group 3 who have manifestations of ASD and epileptic seizures compared to other groups (p<0.05, r=0.99). Conclusions. The data is a reliable evidence that children with ASD, epileptic seizures and epileptiform changes in the EEG have higher levels of omega-6 PUFA and arachidonic acid and omega-3 and docosahexaenoic acid deficiency. The most pronounced imbalance in both the ratio of omega-6 / omega-3 and arachidonic acid / docosahexaenoic acid in the group of children with ASD and epileptiform changes in the EEG. These results may support the theory of changes in the properties of the phospholipid membrane and ion channels of nerve cells and require further study using electron microscopy. The res","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121098482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose - to familiarize general practitioners and pediatricians with the real risks and modern, relevant principles of the observation of children with rheumatic diseases during the COVID-19 pandemic. It was described which risks should be primarily considered in patients with rheumatic diseases during the COVID-19 pandemic. It was described the data from the literature on the safety of treatment of children with rheumatic diseases and the peculiarities of their management against the background of the pandemic of infection, caused by the SARS-CoV-2 virus. It was generalized the data on the purpose and frequency of the laboratory tests depending on the received therapy is given. It was focused on the leading issues of monitoring children with rheumatic diseases, adaptation of these patients in the conditions of the COVID-19 pandemic. It was described in detail some differences in the follow-up of patients receiving biological and basic therapy. Emphasis is placed on issues of the monitoring treatment with methotrexate, which is the most often prescribed in pediatric rheumatology. There were discussed the main features of the infectious syndrome in children with rheumatic diseases depending on the prescribed medications (biological drugs and basic treatment). There were proposed the tactics of the first-line doctors in providing medical care to children with rheumatic diseases in the event of the development of an intercurrent infection. It is noted which changes in the treatment of rheumatic disease should be applied to the rheumatic patient in case of the development of an infectious process. Emphasis is placed on the management of pediatric patients with a rheumatic profile in a case of the contact with a patient with COVID-19, asymptomatic carrier or disease of COVID-19. There were described the issues of the vaccination of children with rheumatic diseases. There were added a new data of agreed modern recommendations on calendar and additional vaccine prophylaxis of children with rheumatic diseases, including vaccination against COVID-19. No conflict of interests was declared by the authors. Keywords: children, rheumatic diseases, management, vaccination, COVID-19.
{"title":"Rheumatic diseases in children during the COVID-19 pandemic: what the pediatrician and family physician should know","authors":"O. Oshlianska, O. Okhotnikova, L. Kvashnina","doi":"10.15574/pp.2022.90.51","DOIUrl":"https://doi.org/10.15574/pp.2022.90.51","url":null,"abstract":"Purpose - to familiarize general practitioners and pediatricians with the real risks and modern, relevant principles of the observation of children with rheumatic diseases during the COVID-19 pandemic. It was described which risks should be primarily considered in patients with rheumatic diseases during the COVID-19 pandemic. It was described the data from the literature on the safety of treatment of children with rheumatic diseases and the peculiarities of their management against the background of the pandemic of infection, caused by the SARS-CoV-2 virus. It was generalized the data on the purpose and frequency of the laboratory tests depending on the received therapy is given. It was focused on the leading issues of monitoring children with rheumatic diseases, adaptation of these patients in the conditions of the COVID-19 pandemic. It was described in detail some differences in the follow-up of patients receiving biological and basic therapy. Emphasis is placed on issues of the monitoring treatment with methotrexate, which is the most often prescribed in pediatric rheumatology. There were discussed the main features of the infectious syndrome in children with rheumatic diseases depending on the prescribed medications (biological drugs and basic treatment). There were proposed the tactics of the first-line doctors in providing medical care to children with rheumatic diseases in the event of the development of an intercurrent infection. It is noted which changes in the treatment of rheumatic disease should be applied to the rheumatic patient in case of the development of an infectious process. Emphasis is placed on the management of pediatric patients with a rheumatic profile in a case of the contact with a patient with COVID-19, asymptomatic carrier or disease of COVID-19. There were described the issues of the vaccination of children with rheumatic diseases. There were added a new data of agreed modern recommendations on calendar and additional vaccine prophylaxis of children with rheumatic diseases, including vaccination against COVID-19. No conflict of interests was declared by the authors. Keywords: children, rheumatic diseases, management, vaccination, COVID-19.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116516312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The formation of myocardial dysfunction in neonatal sepsis remains a topical issue in current neonatal practice. Myocardial dysfunction with neonatal sepsis is found to promote a double increase of neonatal loss rate, and maintenance of an adequate cardiac output is very important predictor for survival of patients with neonatal sepsis. Purpose - to study clinico-paraclinical markers of myocardial dysfunction in case of an alternative term of neonatal sepsis debut for timely verification and rational correction of cardiovascular disorders. Materials and methods. Considering various terms of neonatal sepsis manifestation with its early and late variants, a comparative analysis of clinical-paraclinical markers of generalized infectious-inflammatory process was made in 26 neonates at the stage of obstetrical aid (retrospective analysis) and in the intensive care departments for neonates and their resuscitation (prospective follow-up). According to the results of the analysis of medical records of newborns, the diagnosis of early neonatal sepsis was established in 14 (53.8%) newborns who were included in the clinical group I of the study, and the diagnosis of late neonatal sepsis was established in 12 (46.2%) newborns who were included in the clinical group II. Results. After birth general condition of patients from the group I was assessed as of moderate severity - in 1 (7.1%) and severe - in 13 (92.9%) neonates; in the group II the 2 neonates (16.7%) were in the condition of moderate severity (р>0.05) and 10 (83.3%) neonates were in severe condition (р>0.05). Assessment of cardiovascular constituent in the neonatal groups of comparison according to the constellation scale nSOFA in the means values did not differ reliably, though it was indicative of a tendency to deeper disorders in case of late neonatal sepsis. Conclusions. Sepsis-induced myocardial dysfunction is manifested by increase in the content of cardio-specific markers (creatine phosphokinase, МВ fraction, troponin I). An increased content of troponin I in the blood higher than that of the norm in case of late neonatal sepsis possesses a high inclination (83.3%) to the formation of myocardial dysfunction. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors. Keywords: neonates, neonatal sepsis, myocardial dysfunction.
{"title":"Myocardial dysfunction as a component of multiple organ discrepancy with neonatal sepsis","authors":"N. Kretsu, О. Koloskova, О.О. Shakhova","doi":"10.15574/pp.2022.90.12","DOIUrl":"https://doi.org/10.15574/pp.2022.90.12","url":null,"abstract":"The formation of myocardial dysfunction in neonatal sepsis remains a topical issue in current neonatal practice. Myocardial dysfunction with neonatal sepsis is found to promote a double increase of neonatal loss rate, and maintenance of an adequate cardiac output is very important predictor for survival of patients with neonatal sepsis. Purpose - to study clinico-paraclinical markers of myocardial dysfunction in case of an alternative term of neonatal sepsis debut for timely verification and rational correction of cardiovascular disorders. Materials and methods. Considering various terms of neonatal sepsis manifestation with its early and late variants, a comparative analysis of clinical-paraclinical markers of generalized infectious-inflammatory process was made in 26 neonates at the stage of obstetrical aid (retrospective analysis) and in the intensive care departments for neonates and their resuscitation (prospective follow-up). According to the results of the analysis of medical records of newborns, the diagnosis of early neonatal sepsis was established in 14 (53.8%) newborns who were included in the clinical group I of the study, and the diagnosis of late neonatal sepsis was established in 12 (46.2%) newborns who were included in the clinical group II. Results. After birth general condition of patients from the group I was assessed as of moderate severity - in 1 (7.1%) and severe - in 13 (92.9%) neonates; in the group II the 2 neonates (16.7%) were in the condition of moderate severity (р>0.05) and 10 (83.3%) neonates were in severe condition (р>0.05). Assessment of cardiovascular constituent in the neonatal groups of comparison according to the constellation scale nSOFA in the means values did not differ reliably, though it was indicative of a tendency to deeper disorders in case of late neonatal sepsis. Conclusions. Sepsis-induced myocardial dysfunction is manifested by increase in the content of cardio-specific markers (creatine phosphokinase, МВ fraction, troponin I). An increased content of troponin I in the blood higher than that of the norm in case of late neonatal sepsis possesses a high inclination (83.3%) to the formation of myocardial dysfunction. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors. Keywords: neonates, neonatal sepsis, myocardial dysfunction.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124184135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Infertile marriage is a complex socio-economic problem; the frequency of infertility in Ukraine is 17-20%, which prompts further study of its mechanisms, including immune ones, in order to develop appropriate methods of correction. Purpose - to characterize the phenotypic features of T-cells and their subpopulation of T-helpers (CD3+CD4+) with the expression of intracellular cytokines in women with a history of infertility at different stages of pregnancy. Materials and methods. Relative levels of immunocompetent cells (cl) of blood, including intracellular expression of cytokines CD3+CD4+cells, in 436 non-pregnant (the reference group n) and 514 pregnant women with infertility at the first trimester and the second trimester of pregnancy were determined using a cellular cytofluorimeter and the corresponding test systems. Characteristics of indicators in pregnant women at the different terms in groups were analyzed: a - 4-7 weeks (140 women); b - 8-9 weeks (163 women); c - 10-12 weeks (133 women); d - 13-18 weeks (63 women); e - 19-28 weeks (15 women) compared to the group n and among themselves. Results. The first 12 weeks of pregnancy in women with a history of infertility are characterized by high blood levels of T-helpers (CD3+CD4+-cl), and their activation according to the expression of markers CD25 and HLA-DR, and for 18 weeks - intracellular secretion of pro-inflammatory cytokines IFN-γ + and TNF-α, high expression of anti-inflammatory IL-4 was observed throughout the 27 weeks of follow-up. The percentage of women with high levels in the blood of CD3+CD4+IFN-γ+- and CD3+CD4+TNF-α+-L is the highest during the first 12 weeks, from 13th week these indicators for TNF-α, and with 20th - for IFN-γ do not differ from non-pregnant ones. Since the beginning of pregnancy, the relative number of women with low expression of IL-4 and IL-10 T-helpers has significantly decreased; within 13-18 weeks, the percentage of such pregnant women is significantly increased to the reference group, and such women are the most vulnerable due to the possible weak reaction of the anti-inflammatory link to the state of tolerance. Conclusions. The characteristics of peripheral blood immune system cell levels according to their CD phenotypes and intracellular cytokine expression indicate their participation in pregnancy mechanisms at different stages and individual analysis allows for negative predictions and improvement of pregnancy course in women with a history of infertility. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors. Keywords: CD-phenotype of immunocompetent cells, intracellular cytokines, pregnant women, history of infertility.
{"title":"Features of CD3 +- and CD3+CD4 +-peripheral blood cells at different pregnancy stages in women with anamnese infertility","authors":"L. Tumanova, E.V. Kolomiіets","doi":"10.15574/pp.2022.90.5","DOIUrl":"https://doi.org/10.15574/pp.2022.90.5","url":null,"abstract":"Infertile marriage is a complex socio-economic problem; the frequency of infertility in Ukraine is 17-20%, which prompts further study of its mechanisms, including immune ones, in order to develop appropriate methods of correction. Purpose - to characterize the phenotypic features of T-cells and their subpopulation of T-helpers (CD3+CD4+) with the expression of intracellular cytokines in women with a history of infertility at different stages of pregnancy. Materials and methods. Relative levels of immunocompetent cells (cl) of blood, including intracellular expression of cytokines CD3+CD4+cells, in 436 non-pregnant (the reference group n) and 514 pregnant women with infertility at the first trimester and the second trimester of pregnancy were determined using a cellular cytofluorimeter and the corresponding test systems. Characteristics of indicators in pregnant women at the different terms in groups were analyzed: a - 4-7 weeks (140 women); b - 8-9 weeks (163 women); c - 10-12 weeks (133 women); d - 13-18 weeks (63 women); e - 19-28 weeks (15 women) compared to the group n and among themselves. Results. The first 12 weeks of pregnancy in women with a history of infertility are characterized by high blood levels of T-helpers (CD3+CD4+-cl), and their activation according to the expression of markers CD25 and HLA-DR, and for 18 weeks - intracellular secretion of pro-inflammatory cytokines IFN-γ + and TNF-α, high expression of anti-inflammatory IL-4 was observed throughout the 27 weeks of follow-up. The percentage of women with high levels in the blood of CD3+CD4+IFN-γ+- and CD3+CD4+TNF-α+-L is the highest during the first 12 weeks, from 13th week these indicators for TNF-α, and with 20th - for IFN-γ do not differ from non-pregnant ones. Since the beginning of pregnancy, the relative number of women with low expression of IL-4 and IL-10 T-helpers has significantly decreased; within 13-18 weeks, the percentage of such pregnant women is significantly increased to the reference group, and such women are the most vulnerable due to the possible weak reaction of the anti-inflammatory link to the state of tolerance. Conclusions. The characteristics of peripheral blood immune system cell levels according to their CD phenotypes and intracellular cytokine expression indicate their participation in pregnancy mechanisms at different stages and individual analysis allows for negative predictions and improvement of pregnancy course in women with a history of infertility. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors. Keywords: CD-phenotype of immunocompetent cells, intracellular cytokines, pregnant women, history of infertility.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125259957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose - to improve diagnostics and making multisystem inflammatory syndrome diagnosis in children based on the study of the clinical course and determination of laboratory and instrumental features. Materials and methods. 15 cases of multisystem inflammatory syndrome in children who were hospitalized in the Infectious Diseases Boxed Department No.1 of the Vinnytsya Regional Children’s Clinical Hospital in 2021 were analyzed. Results. The most common symptom was fever. Gastrointestinal disorders (53.33%), neurocognitive and vegetative symptoms (86.67%) were also found in children. Arthralgias, myalgias and pains in the lower extremities were in 33.33% of children, respiratory symptoms - in 20.00% of ones. Almost half of the children had skin rashes at physical examination. Scleritis and conjunctivitis was also noted in the quarter children. Leukocytosis was detected in 8 children (53.33%) in the results of blood count. Majority of children had elevated erythrocyte sedimentation rate, C-reactive protein and fibrinogen. D-dimer was increased in all children. 46.67% of children, according to chest ultrasound had fluid in the pleural cavity up to 7-10 mm, signs of interstitial pulmonary edema, increased echo-signals along the axillary line and the consolidation zone in the middle right lung from 3 mm to 6 mm. 60.0% of children had enlarged liver, spleen, fluid in the abdomen and enlarged multiple mesenteric lymph nodes according abdominal ultrasound. Conclusions. The diagnosis of multisystem inflammatory syndrome should be made according to the main criteria. It requires differential diagnosis with a number of infectious and systemic diseases. Prolonged fever and neurocognitive symptoms were the most common symptoms in hospitalized patients. Gastrointestinal and cardiovascular disorders, skin rashes prevailed. Typical laboratory changes were elevated markers of inflammation, evidence of coagulopathy and markers of previous coronavirus infection. Significant features among echosonographic changes were enlargement of parenchymal organs and signs of effusion in the cavities. Child protection from the impact of coronavirus infection should be improved. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors. Keywords: multisystem inflammatory syndrome, MIS-C, SARS-CoV-2, children.
{"title":"Clinical and laboratory-instrumental characteristic of multisystem inflammatory syndrome in children","authors":"L. Yushchenko, G. Mantak, V. S. Oliynyk","doi":"10.15574/pp.2022.90.44","DOIUrl":"https://doi.org/10.15574/pp.2022.90.44","url":null,"abstract":"Purpose - to improve diagnostics and making multisystem inflammatory syndrome diagnosis in children based on the study of the clinical course and determination of laboratory and instrumental features. Materials and methods. 15 cases of multisystem inflammatory syndrome in children who were hospitalized in the Infectious Diseases Boxed Department No.1 of the Vinnytsya Regional Children’s Clinical Hospital in 2021 were analyzed. Results. The most common symptom was fever. Gastrointestinal disorders (53.33%), neurocognitive and vegetative symptoms (86.67%) were also found in children. Arthralgias, myalgias and pains in the lower extremities were in 33.33% of children, respiratory symptoms - in 20.00% of ones. Almost half of the children had skin rashes at physical examination. Scleritis and conjunctivitis was also noted in the quarter children. Leukocytosis was detected in 8 children (53.33%) in the results of blood count. Majority of children had elevated erythrocyte sedimentation rate, C-reactive protein and fibrinogen. D-dimer was increased in all children. 46.67% of children, according to chest ultrasound had fluid in the pleural cavity up to 7-10 mm, signs of interstitial pulmonary edema, increased echo-signals along the axillary line and the consolidation zone in the middle right lung from 3 mm to 6 mm. 60.0% of children had enlarged liver, spleen, fluid in the abdomen and enlarged multiple mesenteric lymph nodes according abdominal ultrasound. Conclusions. The diagnosis of multisystem inflammatory syndrome should be made according to the main criteria. It requires differential diagnosis with a number of infectious and systemic diseases. Prolonged fever and neurocognitive symptoms were the most common symptoms in hospitalized patients. Gastrointestinal and cardiovascular disorders, skin rashes prevailed. Typical laboratory changes were elevated markers of inflammation, evidence of coagulopathy and markers of previous coronavirus infection. Significant features among echosonographic changes were enlargement of parenchymal organs and signs of effusion in the cavities. Child protection from the impact of coronavirus infection should be improved. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors. Keywords: multisystem inflammatory syndrome, MIS-C, SARS-CoV-2, children.","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130640586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The harmful habit of smoking is an urgent and important socio-medical problem that has become an epidemic, including in Ukraine. It is a matter of concern that more than 500,000 young people join this bad habit every year. Today, smoking is a modified risk factor for the formation and progression of many diseases, including dental pathology among different age groups, especially among teenagers and young adults. The oral cavity is the first barrier to tobacco smoke with toxins and carcinogens that are part of it. It is known that the pathogenesis of many diseases is accompanied by a nonspecific generalized response of the organism in the form of endogenous intoxication (EI) syndrome, the severity of which may be a criterion for the severity of the pathological process and affect its course. Medium-weight molecules (MWM) are a common marker of EI in biological fluids among metabolites that can be used to assess the severity of disease. The universally accepted marker of EI in biological liquids among metabolites, which gives a possibility to assess the severity of the disease, is medium-weight molecules (MWM) - a class, which combines chemically differently structured components with a mass between 500 and 5000 Da and pronounced biologic activity. Purpose - to determine the degree of EI in the oral fluid by the level of MWM in teenagers and young adults who smoke. Materials and methods. It is studied the dental status of 114 teenagers and young adults aged 15 to 24 years, which was divided into groups: group I included 26 people who regularly smoke traditional cigarettes; group II - 22 people who regularly smoke electronic cigarettes (Vapes); group III - 23 people who regularly smoke tobacco heating devices (IQOS); group IV - 43 people without a bad habit of smoking. The degree of EI was determined by the rate of MWM in oral fluid by the express method according to a modified method Gabrielyan NI et al., 1984. Results. The analyze of the rate in the oral fluid of peptide residues (MWM254) in persons of the group I was exhibited 1.4 times more than in persons of the group IV (p<0.001). There was a similar tendency in the other groups - the group I and the group II had 1.3 times more MWM254 (p<0.05) and 1.2 times more (p<0.001). There was also a difference in the nucleotide fillings (MWM280) in oral fluid of the study groups. Thus, in persons of the group I it was found MWM280 1.6 times more than in persons of the group IV (p<0.001) and 1.3 times more (p<0.05) in persons of the groups II and III respectively. The increase of nucleotide-peptide index was determined depending on the presence and type of malodorous behavior in the study participants. Conclusions. The obtained results indicate the development of EI in teenagers and young adults who smoke, as indicated by the increase in the level of MWM in the oral fluid in the subjects, a marker of endotoxicosis. It was found that the degree of endogenous intoxication depends on the type of smoking, as
吸烟的有害习惯是一个紧迫和重要的社会医学问题，已成为一种流行病，包括在乌克兰。每年有50多万年轻人染上这种坏习惯，这是一个令人担忧的问题。今天，吸烟是许多疾病形成和发展的一个危险因素，包括不同年龄组，特别是青少年和年轻人的牙齿病理。口腔是抵御含有毒素和致癌物质的烟草烟雾的第一道屏障。众所周知，许多疾病的发病机制都伴随着机体以内源性中毒(EI)综合征形式的非特异性全身性反应，其严重程度可能是病理过程严重程度的一个标准，并影响其病程。中等重量分子(MWM)是生物体液代谢产物中常见的EI标记物，可用于评估疾病的严重程度。在生物液体代谢物中，普遍接受的EI标记物是中等重量分子(MWM)，这类分子结合了化学结构不同的成分，质量在500至5000 Da之间，具有明显的生物活性，可以评估疾病的严重程度。目的-通过吸烟的青少年和年轻成年人的MWM水平来确定口腔液中EI的程度。材料和方法。研究了114名15 ~ 24岁的青少年和青壮年的牙齿状况，将其分为两组:一组26人经常抽传统香烟;第二组——22名经常吸电子烟的人;第三组- 23人经常使用烟草加热装置(IQOS);第四组:43人没有吸烟的坏习惯。根据Gabrielyan NI et al.， 1984的改进方法，用快速法测定口服液中MWM的含量来测定EI的程度。结果。ⅰ组人口服液中肽残基(MWM254)的分析率是ⅳ组人的1.4倍(p<0.001)。其他组也有类似的趋势，I组和II组的MWM254分别是1.3倍(p<0.05)和1.2倍(p<0.001)。研究组口服液中的核苷酸填充物(MWM280)也存在差异。因此，在I组中，MWM280分别是IV组的1.6倍(p<0.001)和III组的1.3倍(p<0.05)。核苷酸-肽指数的增加取决于研究参与者的气味行为的存在和类型。结论。所获得的结果表明，吸烟的青少年和年轻人的EI的发展，正如受试者口服液中MWM水平的增加所表明的那样，这是内毒素中毒的标志。结果发现，内源性中毒的程度取决于吸烟类型，以及病理过程的发展程度。这项研究是按照《赫尔辛基宣言》的原则进行的。研究方案经参与机构当地伦理委员会批准。获得患者的知情同意进行研究。作者未声明存在利益冲突。关键词:青少年，内源性中毒，中重分子，吸烟。
{"title":"Determination of endogenous intoxication in teenagers and young adults who smoke","authors":"I. Lisetska, M. Rozhko","doi":"10.15574/pp.2022.90.39","DOIUrl":"https://doi.org/10.15574/pp.2022.90.39","url":null,"abstract":"The harmful habit of smoking is an urgent and important socio-medical problem that has become an epidemic, including in Ukraine. It is a matter of concern that more than 500,000 young people join this bad habit every year. Today, smoking is a modified risk factor for the formation and progression of many diseases, including dental pathology among different age groups, especially among teenagers and young adults. The oral cavity is the first barrier to tobacco smoke with toxins and carcinogens that are part of it. It is known that the pathogenesis of many diseases is accompanied by a nonspecific generalized response of the organism in the form of endogenous intoxication (EI) syndrome, the severity of which may be a criterion for the severity of the pathological process and affect its course. Medium-weight molecules (MWM) are a common marker of EI in biological fluids among metabolites that can be used to assess the severity of disease. The universally accepted marker of EI in biological liquids among metabolites, which gives a possibility to assess the severity of the disease, is medium-weight molecules (MWM) - a class, which combines chemically differently structured components with a mass between 500 and 5000 Da and pronounced biologic activity. Purpose - to determine the degree of EI in the oral fluid by the level of MWM in teenagers and young adults who smoke. Materials and methods. It is studied the dental status of 114 teenagers and young adults aged 15 to 24 years, which was divided into groups: group I included 26 people who regularly smoke traditional cigarettes; group II - 22 people who regularly smoke electronic cigarettes (Vapes); group III - 23 people who regularly smoke tobacco heating devices (IQOS); group IV - 43 people without a bad habit of smoking. The degree of EI was determined by the rate of MWM in oral fluid by the express method according to a modified method Gabrielyan NI et al., 1984. Results. The analyze of the rate in the oral fluid of peptide residues (MWM254) in persons of the group I was exhibited 1.4 times more than in persons of the group IV (p<0.001). There was a similar tendency in the other groups - the group I and the group II had 1.3 times more MWM254 (p<0.05) and 1.2 times more (p<0.001). There was also a difference in the nucleotide fillings (MWM280) in oral fluid of the study groups. Thus, in persons of the group I it was found MWM280 1.6 times more than in persons of the group IV (p<0.001) and 1.3 times more (p<0.05) in persons of the groups II and III respectively. The increase of nucleotide-peptide index was determined depending on the presence and type of malodorous behavior in the study participants. Conclusions. The obtained results indicate the development of EI in teenagers and young adults who smoke, as indicated by the increase in the level of MWM in the oral fluid in the subjects, a marker of endotoxicosis. It was found that the degree of endogenous intoxication depends on the type of smoking, as","PeriodicalId":330226,"journal":{"name":"UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127053560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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