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Diversity of Ordo Lepidoptera In Mangkok Resort, Sebangau National Park, Central Kalimantan 中加里曼丹Sebangau国家公园Mangkok度假村鳞翅目动物的多样性
Pub Date : 2018-12-30 DOI: 10.20956/IJAB.V2I2.5815
A. Faqih
The diversity of the butterfly in Sebangau National Park, particularly in the Mangkok Resort area has different morphological features in each species. The differences distinguish each species between families. Steps to know the diversity species or populations can be measured and identified based on similarities or differences in Shannon-Wiener's diversity analysis. This research was conducted by random purposive sampling using insect net and food trap. Observation and sampling were conducted in the area of natural forest and forest restoration during the dry and rainy seasons. The results showed, the species obtained and identified as many as 25 species from 4 families namely Papilionidae, Nymphalidae, Pieridae and Lycaenidae. Butterflies obtained from food trap are 24 individuals from 5 species. The level of diversity, evenness and richness of the species of butterflies in each region during the rainy and dry seasons which are the location of the study are included in the "medium" category with the average value of the diversity index, the evenness and the richness of the butterfly species of 2.502. The relative dominance and relative abundance of the butterfly species in the Mangkok resort shows no species communities that are extreme dominant from species other.
Sebangau国家公园,特别是Mangkok度假区蝴蝶的多样性在每个物种中都有不同的形态特征。不同的物种在不同的科之间有区别。了解多样性物种或种群的步骤可以根据Shannon Wiener多样性分析中的相似性或差异进行测量和识别。本研究采用有目的的随机抽样方法,使用防虫网和食物捕捉器进行。在旱季和雨季,在天然林和森林恢复区进行了观测和采样。结果表明,该物种共鉴定出蝶科、睡蝶科、粉蝶科、石蝶科4科25种。从食物捕捉器中获得的蝴蝶有来自5个物种的24个个体。研究所在地的每个地区在雨季和旱季的蝴蝶物种多样性、均匀性和丰富度水平均属于“中等”类别,蝴蝶物种的多样性指数、均匀度和丰富度的平均值为2.502。Mangkok度假村蝴蝶物种的相对优势和相对丰度表明,没有任何物种群落比其他物种具有极端优势。
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引用次数: 0
Ligation, Transformation and Characterization of Rv 1926c Mycobacterium tuberculosis to Escherichia coli JM 109 For Latent Tuberculosis Immunodiagnostic 结核分枝杆菌Rv 1926c与大肠杆菌JM109的连接、转化及特性研究
Pub Date : 2018-12-30 DOI: 10.20956/IJAB.V2I2.5813
R. Agus
Tuberculosis caused by Mycobacterium tuberculosis is the biggest infectious disease causing human death in the world. The main challenge in controlling tuberculosis is to quickly and accurately diagnose tuberculosis infection. Several kits have been produced to diagnose tuberculosis, but have different sensitivity and specificity. This shows that the kit is not yet ideal for diagnosing tuberculosis, so the search for candidates for specific antigens still needs to be done. One potential antigen is the Rv 1926c encoding MPT 63 protein. This protein is known to induce Th1 cells and produce IFN λ from PBMC cells of patients infected with tuberculosis. The purpose of this study was to clone the Rv 1926c from Mycobacterium tuberculosis as a tuberculosis immunodiagnostic kit. The method used is isolating Rv 1926c with PCR, ligation to pGEM-T vector and transformation to E.coli host cell JM 109. Clone characterization was carried out by PCR and migration analysis. The results obtained are the recombinant clones obtained have successfully inserted with the Rv 1926 c
由结核分枝杆菌引起的结核病是世界上导致人类死亡的最大传染病。控制结核病的主要挑战是快速准确地诊断结核病感染。已经生产了几种诊断结核病的试剂盒,但灵敏度和特异性不同。这表明该试剂盒尚不适合诊断结核病,因此仍需寻找特异性抗原的候选者。一种潜在的抗原是编码MPT63蛋白的Rv 1926c。已知该蛋白可诱导Th1细胞,并从感染结核病的患者的PBMC细胞中产生IFNλ。本研究的目的是从结核分枝杆菌中克隆Rv 1926c作为结核病免疫诊断试剂盒。所使用的方法是用PCR分离Rv 1926c,连接到pGEM-T载体并转化到大肠杆菌宿主细胞JM 109。通过PCR和迁移分析进行克隆鉴定。所获得的结果是所获得的重组克隆已成功插入Rv 1926 c
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引用次数: 0
Vegetation structure, species diversity, and mangrove zonation patterns in the Tanjung Panjang Nature Reserve Area, Gorontalo, Indonesia 印尼哥伦达洛丹戎班江自然保护区植被结构、物种多样性和红树林地带性格局
Pub Date : 2018-12-25 DOI: 10.20956/ijab.v2i2.5752
D. W. Baderan, R. Utina, Nurain Lapolo
This study aimed to analyze the structure of vegetation, diversity and patterns of mangrove zonation in the Tanjung Panjang Nature Reserve area. Data collection of mangrove vegetation structure was carried out by using the line transect method (plot measuring 20 m x 20 m), measurement of environmental parameters that supported mangrove life, and 12 soil samples carried out by laboratory tests. The sample distribution was 18 research sample points. The results showed that mangrove vegetation density both at the level of trees, saplings and seedlings was in the category of total damage with density values at tree level 0.04-0.22 ind/ha), sapling 0- 0.07ind/ha), and seedlings 0.02-0.08 ind/Ha). The percentage of closure types ranged from 0% - 38%, meaning that the criteria of damage was rare, which was <50%, and some were included in the criteria of total damage, which was 0%. The index of mangrove species diversity was sequential starting from the level of trees, saplings and seedlings, which were 0.37-1.53, 0.00-0.31, and 0.00, where the tree level diversity index was in the medium category, and the sapling and seedling levels were in the low category.
本研究旨在分析丹戎班江自然保护区的植被结构、多样性和红树林带格局。采用样线法(样地面积为20 m × 20 m)收集红树林植被结构数据,测量支持红树林生命的环境参数,并通过实验室测试采集12个土壤样品。样本分布为18个研究样本点。结果表明:红树林乔木、幼树和幼苗水平的植被密度均处于总损害范畴,密度值分别为乔木水平0.04 ~ 0.22 ind/ha、幼树水平0 ~ 0.07ind/ha和幼苗水平0.02 ~ 0.08 ind/ha;闭合类型的百分比在0% ~ 38%之间,这意味着损伤标准很少,小于50%,一些闭合类型包含在总损伤标准中,为0%。红树林物种多样性指数从乔木、幼树和幼苗水平依次为0.37 ~ 1.53、0.00 ~ 0.31和0.00,其中乔木水平多样性指数处于中等水平,幼树和幼苗水平多样性指数处于低水平。
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引用次数: 9
Bioaccumulation Monitoring of Heavy Metal Chromium (Cr) in Fish Oreochromis mossambicus in The Floating Net Cultivation in Sidoarjo Krembangan River Sidoarjo Krembangan河浮网养殖中罗非鱼体内重金属铬的生物累积监测
Pub Date : 2018-12-25 DOI: 10.20956/IJAB.V2I2.5089
Sonny Kristianto
ABSTRACTFloating Net is a simple alternative cultivation methods. The river there is a garden in the Krembangan District Sidarjoi which is used as a place of cultivation of  Floating net fish oreochromis mossambicus (Oreochromis mossambicus), on the other hand is where waste disposal by the local community and some industries . This research aims to know the accumulation of heavy metals chromium (Cr) in the flesh of fish oreochromis mossambicus (Oreochromis mossambicus) and find out the feasibility of fish consumption oreochromis mossambicus at floating net on the river Krembangan Sidoaarjo. This research are observatif by using Purposive Random Sampling techniques. test the content of heavy metal chromium (Cr) in the flesh of fish oreochromis mossambicus (Oreochromis mossambicus) method using AAS (Atomic Absorbtion Spectrofotometry). The data generated in the analysis in quantitative descriptive.  In this research show that the heavy metal content of chromium (Cr) in the flesh of fish oreochromis mossambicus highs station III of 0.52 mg/Kg; I station of 0.51 mg/Kg II station of 0.48 mg/Kg on a sampling sedangkang the morning, at night the highest value on the station III of 0.50 mg/Kg; I station of 0.46 mg/Kg and the II of 0.39 mg/Kg. Average deposits of heavy metal Cr on fish oreochromis mossambicus (o. mossambicus) is still under the raw quality of the already established by the Director-General of POM 1989 i.e. amounted to 2.5 mg/kg but fall into the category bioakumulasi medium, so that fish oreochromis mossambicus is still worthy of consumption, but need to watch out for, considering the Cr are toxic, carcinogenic, bioakumulatif and biomagnifikasi would potentially interfere with health.  
ABSTRACT浮网是一种简单的替代栽培方法。这条河在Krembangan区Sidarjoi有一个花园,用作养殖浮网鱼oreochromis mossambicus(Oreochromismossambius)的地方,另一方面也是当地社区和一些行业处理废物的地方。本研究旨在了解墨鱼(oreochromis mossambicus)肉中重金属铬(Cr)的积累,并探讨在Krembangan Sidoarjo河浮网中食用墨鱼的可行性。本研究采用目的随机抽样技术进行观察。用原子吸收光谱法测定石首鱼(oreochromis mossambicus)鱼肉中重金属铬的含量。在分析中产生的数据采用定量描述性。本研究表明,该鱼肉中铬(Cr)的重金属含量高达0.52mg/Kg;Ⅰ站为0.51毫克/公斤Ⅱ站为0.48毫克/公斤,在早晨一次取样色当康,夜间最高值在Ⅲ站为0.50毫克/公斤;I站为0.46mg/Kg,II站为0.39mg/Kg。莫桑比克罗非鱼(o.mossambicus)上重金属Cr的平均沉积量仍低于POM 1989总干事已经确定的原始质量,即2.5 mg/kg,但属于生物库穆拉西培养基类别,因此莫桑比克罗非鱼仍然值得食用,但需要注意,因为铬是有毒、致癌的,生物累积和生物放大可能会干扰健康。
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引用次数: 0
Identification of Pseudomonas fluorescens using different biochemical tests 不同生化试验鉴定荧光假单胞菌
Pub Date : 2018-12-25 DOI: 10.20956/IJAB.V2I2.5260
Bikram Nepali, S. Bhattarai, J. Shrestha
Pseudomonas fluorescens not only enhances the plant growth but also controls the fungal pathogens by production of anti fungal metabolites. The objective of this experiment was to identify P. fluorescens using different biochemical tests. This research was carried out in Plant Pathology Laboratory at Agriculture and Forestry University (AFU), Rampur, Chitwan, Nepal. The result of this experiment indicated that P.  fluorescens gave positive result for Catalase test, Gelatin liquefaction, Fluorescent pigment and Oxidase test but negative result for starch hydrolysis test. The colony of P. fluorescens was maximum in maize seed than that of rice seed after two hours of inoculation, whereas higher number of colony was found in rice seed than that of maize seed after twenty four hours of seed inoculation. This findings is useful for identifying colony of P. fluorescens  per seed which is necessary for better seedling growth and effective biological control of pathogens.
荧光假单胞菌不仅能促进植物生长,还能通过产生抗真菌代谢产物来控制真菌病原体。本实验的目的是使用不同的生化测试来鉴定荧光假单胞菌。这项研究是在尼泊尔奇特万拉姆普尔农林大学植物病理学实验室进行的。实验结果表明,荧光假单胞菌在过氧化氢酶试验、明胶液化试验、荧光色素试验和氧化酶试验中均呈阳性,而在淀粉水解试验中呈阴性。接种2小时后,荧光假单胞菌在玉米种子中的菌落数大于水稻种子,而接种24小时后,水稻种子中的细菌菌落数高于玉米种子。这一发现有助于鉴定每个种子的荧光假单胞菌菌落,这对于更好的幼苗生长和有效的病原体生物控制是必要的。
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引用次数: 8
Agronomic performance and genotypic diversity for morphological traits among early maize genotypes 早期玉米形态性状的农艺性能和基因型多样性
Pub Date : 2018-12-25 DOI: 10.20956/IJAB.V2I2.5633
B. Adhikari, J. Shrestha, Bishal Dhakal, B. Joshi, Naba Raj Bhatta
Detailed information on the genetic diversity between maize germplasm (Zea mays L.) is useful for their systematic and efficient use in breeding programs. Fourteen early maize genotypes were studied to assess their performance and genotypic diversity at Doti, Nepal in 2015. Days to tasseling, days to silking, plant height, ear height, ear length, ear diameter and grain yield were significant among genotypes. Genotype SO3TEY-PO-BM, COMPOL-NIOBP and ACROSS-99402 were found higher yielder with earlier maturity. Days to tasseling (0.85), days to silking (0.82), plant height (0.79), ear length (0.71) and ear diameter (0.66) were found highly heritable traits. Grain yield (0.39) and ear height (0.47) medium and remaining traits showed low heritability. High PCV was observed for grain yield (35.10%), number of plants/plot (34.46%), tesseling silking interval (26.85%), harvested ears/plot (24.45%) and husk cover rating (22.85%) where other traits showed medium to low PCV. Grain yield showed high GCV (21.96%), ear height and husk cover had medium and remaining traits showed low GCV (<10%). Plant height (r₌0.498), harvested plants/plot (r₌0.412), harvested ear/plot (r₌0.762), ear length (r₌0.472) and ear diameter (r₌0.470) showed significant positive correlation with grain yield. The yield can be improved if selection applied in favor of those yield components.
玉米种质(Zea mays L.)之间遗传多样性的详细信息有助于其在育种计划中的系统和有效利用。2015年,在尼泊尔多蒂对14种早期玉米基因型进行了研究,以评估其表现和基因型多样性。离穗天数、离丝天数、株高、穗高、穗长、穗径和籽粒产量在各基因型间具有显著性差异。基因型SO3TEY-PO-BM、COMPOL-NIOBP和ACROSS-99402的产量越高,成熟度越早。雄穗天数(0.85)、吐丝天数(0.82)、株高(0.79)、穗长(0.71)和穗径(0.66)具有高度遗传性。产量(0.39)和穗高(0.47)中等和剩余性状的遗传力较低。在籽粒产量(35.10%)、株数/小区(34.46%)、吐丝间隔(26.85%)、收获穗/小区(24.45%)和外壳覆盖率(22.85%)方面观察到较高的PCV,而其他性状表现出中等至较低的PCV。籽粒产量GCV较高(21.96%),穗高和谷壳覆盖率中等,其余性状GCV较低(<10%)。植物高度(r₌0.498),收获的植物/地块(r₌0.412),收获穗/地块(r₌0.762),穗长(r₌0.472)和耳部直径(r₌0.470)与产量呈显著正相关。如果选择有利于这些产量成分,产量可以提高。
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引用次数: 15
Productivity of maize (Zea mays L.) as affected by varieties and sowing dates 玉米品种和播期对产量的影响
Pub Date : 2018-12-25 DOI: 10.20956/IJAB.V2I2.4673
D. B. Gurung, B. Bhandari, J. Shrestha, M. Tripathi
Genotypic yield potential of maize varieties is greatly affected by sowing dates. In order to investigate the effects of sowing dates and varieties on the grain yield of maize, the field experiment was carried out at research field of National Maize Research Program (NMRP), Rampur, Chitwan, Nepal from April 2009 to March 2010.  Three varieties namely Rampur Composite, Arun-2 and Gaurav were sown at every week. The results of experiment showed that interaction effect of variety and sowing date on grain yield of maize was significant. Rampur Composite produced highest grain yield (6.1 t/ha) in August and lowest yield (2.6 t/ha) in May. Similarly Arun-2 produced highest yield (4.6 t/ha) in August and lowest yield (2.1 t/ha) in May. Gaurav produced highest grain yield (5.1 t/ha) in September followed by 4.9, 4.8 and 4.6 t/ha in February, July and August respectively and lowest yield (1.5 t/ha) in November. The sowing date was highly significant on grain production. The highest grain production was 5.1 t/ha in August followed by in February (4.9 t/ha), September (4.6 t/ha) and March (4.4 t/ha) respectively. The lowest grain yield was produced in May (2.4 t/ha). Therefore it was concluded that August planting was best for higher grain production of maize varieties (Rampur Composite, Arun-2 and Gaurav) in terai region of Nepal.
玉米品种的基因型产量潜力受播种日期的影响很大。为了研究播种日期和品种对玉米产量的影响,于2009年4月至2010年3月在国家玉米研究计划(NMRP)位于尼泊尔奇特旺拉姆普尔的研究田进行了田间试验,每周播种拉姆普尔复合玉米、阿伦-2玉米和高拉夫玉米三个品种。试验结果表明,品种和播期对玉米产量的交互作用显著。Rampur Composite在8月产量最高(6.1吨/公顷),5月产量最低(2.6吨/公顷。播种日期对粮食生产具有重要意义。粮食产量最高的是8月份的5.1吨/公顷,其次是2月份(4.9吨/公顷)、9月份(4.6吨/公顷。5月份粮食产量最低(2.4吨/公顷)。因此,得出的结论是,在尼泊尔泰莱地区,8月份种植玉米最有利于提高玉米品种(Rampur Composite、Arun-2和Gaurav)的粮食产量。
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引用次数: 6
The Potential of Algae Spyrogyra peipingensis to Produce Nanogold in Batch Culture Medium 北平Spyrogyra藻类在分批培养基中生产纳米金的潜力
Pub Date : 2018-07-28 DOI: 10.20956/IJAB.V2I1.4450
Arbaina Syahdinnur, Agusrahman Ekaputra Abas, Chitriani Armidha
The biosynthesis of gold nanoparticles by using algae Spirogyra peipingensis was conducted. This research aimed to determine biosynthesis of gold nanoparticles by using algae Spirogyra peipingensis with different concentration and incubation time. The synthesis of gold nanoparticles using HauCl4 solution with variations of concentration 5 ppm, 10 ppm, 15 ppm and 20 ppm respectively in 1 liter of aquabidest. Algae Spirogyra peipingensis was grown in HauCl4 medium with the addition of 0.2 gr Sulfahri-01 nutrient. Then each 5 gram Spirogyra algae incubated in HAuCl4 medium with the addition of Sulfahri-01 0.2 gram nutrients. 4 hours long incubation under exposure to sunlight. Nanoparticle size determination is done by looking at the color that appears in the solution. From the results of the research, it is known that Spirogyra peipingensis algae is able to synthesize gold nanoparticles characterized by the color change in algae biomass from green to purple color after being treated and forming gold nanoparticles with size 40-60 NM. The best-used HAuCl4 consent is 5 ppm with the smallest particle size. KeywordIntracelullar, Spirogyra peipingensis,HAuCl4,Biosynthesis.  Article HistoryReceived 8 May 2017 Accepted 3 July 2017                                                                               
利用北平螺旋体藻进行了纳米金的生物合成。本研究旨在利用不同浓度和培养时间的北平螺旋藻来测定金纳米颗粒的生物合成。使用HauCl4溶液合成金纳米颗粒,在1升坐浴盆中浓度分别为5ppm、10ppm、15ppm和20ppm。在添加了0.2克Sulfahri-01营养素的HauCl4培养基中生长了北平螺藻。然后,每5克Spirogyra藻类在添加了0.2克Sulfahri-01营养物质的HAuCl4培养基中孵育。在阳光下培养4小时。纳米粒子尺寸的测定是通过观察溶液中出现的颜色来完成的。研究结果表明,北平螺旋藻能够合成金纳米粒子,其特征是经过处理后,藻类生物量由绿色变为紫色,形成大小为40-60nm的金纳米粒子。最佳使用的HAuCl4浓度为5ppm,颗粒尺寸最小。关键词洗脱液内,北平螺旋体,HAuCl4,生物合成。文章历史接收日期2017年5月8日接受日期2017年7月3日
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引用次数: 0
Water Feasibility Study of Bengawan Solo River for Irrigation: The Need for Technology to Solve Rice Field Pollution in Sragen, Indonesia 班加湾梭罗河灌溉用水可行性研究:解决印尼斯拉格根稻田污染的技术需求
Pub Date : 2018-07-28 DOI: 10.20956/IJAB.V2I1.3971
S. Prajoko
The aims of this study are to determine the feasibility of Bengawan Solo River water for irrigation of rice field in Sragen, Central Java and to find solution to the problem. The data used in this study were obtained from various literatures related to the issues discussed. The collected data were then selected and analyzed with descriptive argumentative. The result indicates that the water quality of Bengawan Solo River has dropped below the water quality standard grade IV according to Government Regulation Number 82 Year 2001. The use of water from this river has caused the quality of rice to decline below the rice quality standard according to Indonesian National Standard (SNI) 01.6128-2008 due to the bioaccumulation of heavy metals on rice crops, i.e. copper (Cu), mercury (Hg), cadmium (Cd), and Chromium (Cr) exceeded the threshold. Solution proposed to overcome this problem is with counseling programs to farmers conducted by relevant agencies on the use of rice field pollution control technology by physics, chemistry, and biology.
本研究的目的是确定Bengawan Solo河水灌溉中爪哇Sragen稻田的可行性,并找到解决问题的方法。本研究中使用的数据来自与所讨论问题相关的各种文献。然后选择收集的数据,并用描述性论证进行分析。结果表明,Bengawan Solo河的水质已降至2001年第82号政府法规规定的四级水质标准以下。由于重金属在水稻作物上的生物累积,即铜(Cu)、汞(Hg)、镉(Cd)和铬(Cr)超过了阈值,因此使用该河流的水导致水稻质量下降至低于印度尼西亚国家标准(SNI)01.6128-2008规定的水稻质量标准。为解决这一问题,建议相关机构向农民提供物理、化学和生物等方面的稻田污染控制技术使用咨询计划。
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引用次数: 1
Effect planting dates and varieties on infestation of maize stem borer Chilo partellus (Swinhoe) 种植日期和品种对玉米茎螟虫侵染的影响
Pub Date : 2018-07-28 DOI: 10.20956/ijab.v2i1.4512
P. Thakur, G. Bhandari, J. Shrestha, Buddhi B. Achhami
Studies on the effect of different planting dates and varieties of maize on the infestation of maize stem borer (Chilo partellus) were carried out at research field of National Maize Research Program, Rampur, Chitwan, Nepal in  2010/11 and 2011/12. Three maize varieties namely namely Arun-2, Rampur Composite and Gaurav (hybrid) were grown at weekly interval upto 52 weeks from January to December. The results revealed that the damage was higher in Arun-2 (38.9%) followed by Gaurav (35.4%) and Rampur Composite (30.8%) respectively. Higher damage was observed in April (58.2%) and the damage was gradually decreased toward the month of January/February (13.2 to 25.6%) and November/December (13.3 to 16.5%). Lower infestation was observed in winter season (22.5%) followed by spring (47.1%) and summer season (47.0%) respectively. This study suggests that the percent infestation of maize stem borer can be minimized by planting Rampur Composite variety in winter season.
2010/11年和2011/12年,在尼泊尔奇特旺拉姆普尔国家玉米研究计划的研究区,研究了不同种植日期和玉米品种对玉米玉米螟(Chilo partellus)侵扰的影响。从1月到12月,三个玉米品种,即Arun-2、Rampur Composite和Gaurav(杂交),每周间隔52周种植。结果表明,Arun-2(38.9%)的损伤程度更高,其次是Gaurav(35.4%)和Rampur复合材料(30.8%)。4月的虫害率较高(58.2%),1月/2月(13.2%-25.6%)和11月/12月(13.3%-16.5%)的虫害率逐渐下降。冬季(22.5%)虫害率较低,其次是春季(47.1%)和夏季(47.0%)。这项研究表明,在冬季种植Rampur复合品种可以最大限度地减少玉米玉米螟的侵扰率。
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引用次数: 3
期刊
International Journal of Applied Biology
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