Pub Date : 2019-11-02DOI: 10.3390/mol2net-05-06381
Ricardo S. P. Patiño, David Salazar-Valenzuela, Evencio Joel Medina Villamizar, N. G. Mogollón, Carolina Proaño-Bolaños, J. R. Almeida
In Ecuadorian Amazon, Bothrops atrox is the main snake associated with ophidic accidents. The only approved treatment for snakebite envenomation are antivenoms and their efficiency depends on the reference venom used in their production. It is important to mention that B. atrox is a species with wide geographic distribution, which can generate differences in the composition and phenotype of the venom between its different populations. This variability can be responsible for changes in the toxicological effects of snakebite among populations of the same species. With this in mind, the present study analyzed two venom samples from individuals from two Amazonian areas. Based on the results, differences in the abundance of the main families of toxins and their activities between both venoms were identified
{"title":"Comparative study of biochemical composition and enzymatic activity of Bothrops atrox venom","authors":"Ricardo S. P. Patiño, David Salazar-Valenzuela, Evencio Joel Medina Villamizar, N. G. Mogollón, Carolina Proaño-Bolaños, J. R. Almeida","doi":"10.3390/mol2net-05-06381","DOIUrl":"https://doi.org/10.3390/mol2net-05-06381","url":null,"abstract":"In Ecuadorian Amazon, Bothrops atrox is the main snake associated with ophidic accidents. The only approved treatment for snakebite envenomation are antivenoms and their efficiency depends on the reference venom used in their production. It is important to mention that B. atrox is a species with wide geographic distribution, which can generate differences in the composition and phenotype of the venom between its different populations. This variability can be responsible for changes in the toxicological effects of snakebite among populations of the same species. With this in mind, the present study analyzed two venom samples from individuals from two Amazonian areas. Based on the results, differences in the abundance of the main families of toxins and their activities between both venoms were identified","PeriodicalId":337320,"journal":{"name":"Proceedings of MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133159315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-02DOI: 10.3390/mol2net-05-06382
M. Torres, D. Mollocana, M. Erazo, X. Aguirre
{"title":"Molecular characterization of Moniliophthora roreri, causative agent of moniliasis in cocoa in three provinces of Ecuador: Los Ríos, Manabi and Santo Domingo de los Tsáchilas","authors":"M. Torres, D. Mollocana, M. Erazo, X. Aguirre","doi":"10.3390/mol2net-05-06382","DOIUrl":"https://doi.org/10.3390/mol2net-05-06382","url":null,"abstract":"","PeriodicalId":337320,"journal":{"name":"Proceedings of MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134255115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-02DOI: 10.3390/mol2net-05-06380
W. Caicedo, F. N. Ferreira, D. Viáfara, J. Olivares, Alexander Arias, J. Barrionuevo, Luis Oña, David Naula
To evaluate the chemical composition of the taro tuber ( Colocasia esculenta (L) Schott) of waste with and without processing for use in pig diets. In samples of natural tubers, solid state fermentation (SEF) and flour, it was determined: dry matter (DM), organic matter (OM), crude protein (CP), crude fiber (CF), ash, extract ethereal (EE), nitrogen-free extracts (NFE), gross energy (GE), digestible energy (DE) and metabolizable energy (ME). The experiment was conducted according to a completely randomized design, in the necessary cases the means were contrasted with the Duncan test with (p<0.05). The major (p<0.05) content in DM presented the flour. In relation to the OM, the highest contents were evidenced in the natural tuber and in flour. As for CP and ashes, the SEF had the best (p<0.05) contents. As for the CF and NFE, there were no differences (p>0.05) between the unprocessed and processed tuber. Regarding EE, GE, DE and ME, the natural tuber had the highest (p<0.05) concentrations, respectively. In conclusion, processed waste taro tubers constitute a good source of nutrients, guaranteeing a local alternative food of adequate nutritional characteristics for use in pigs.
{"title":"Chemical composition of the taro tuber (Colocasia esculenta L. Schott) of waste with and without processing for use in pigs","authors":"W. Caicedo, F. N. Ferreira, D. Viáfara, J. Olivares, Alexander Arias, J. Barrionuevo, Luis Oña, David Naula","doi":"10.3390/mol2net-05-06380","DOIUrl":"https://doi.org/10.3390/mol2net-05-06380","url":null,"abstract":"To evaluate the chemical composition of the taro tuber ( Colocasia esculenta (L) Schott) of waste with and without processing for use in pig diets. In samples of natural tubers, solid state fermentation (SEF) and flour, it was determined: dry matter (DM), organic matter (OM), crude protein (CP), crude fiber (CF), ash, extract ethereal (EE), nitrogen-free extracts (NFE), gross energy (GE), digestible energy (DE) and metabolizable energy (ME). The experiment was conducted according to a completely randomized design, in the necessary cases the means were contrasted with the Duncan test with (p<0.05). The major (p<0.05) content in DM presented the flour. In relation to the OM, the highest contents were evidenced in the natural tuber and in flour. As for CP and ashes, the SEF had the best (p<0.05) contents. As for the CF and NFE, there were no differences (p>0.05) between the unprocessed and processed tuber. Regarding EE, GE, DE and ME, the natural tuber had the highest (p<0.05) concentrations, respectively. In conclusion, processed waste taro tubers constitute a good source of nutrients, guaranteeing a local alternative food of adequate nutritional characteristics for use in pigs.","PeriodicalId":337320,"journal":{"name":"Proceedings of MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition","volume":"144 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127298432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-02DOI: 10.3390/mol2net-05-06383
C. Lozano
. Nowadays, researching of steroidal saponins (SE) is not present in Ecuador, a country where there is a less the science investment; in spite of owning endemic flora unexplored like Agave americana subps. andina (or the famous “penco azul”); it is a great specie for phytochemical and biotechnician studies. On the other hand, China has been the major SE productor since the end of twentieth century, and actuality, it progresses in prodrugs enhance. For this reason, the author of this short-paper explains how Ecuadorian biotechnicians can do an experimental design with a novel method of enzymatic hydrolysis. This innovative procedure uses a potato-dextrose subtract, which is a culture medium for yeasts of chaguarmishki. This method is useful for extracting O-glucuronidase, an enzyme that catalyze O-glycosides. These molecules are the active agent on Agave genus. Finally, the importance of this research is not for the advantages of SE on pharmaceutics or its human body effect; really, the principal benefit is the enhance of Ecuadorian pharmaceutical industry through a mass production with an investment on science investigation.
{"title":"Steroidal saponins isolation of Agave americana subsp. andina by enzymatic hydrolysis: a new approach.","authors":"C. Lozano","doi":"10.3390/mol2net-05-06383","DOIUrl":"https://doi.org/10.3390/mol2net-05-06383","url":null,"abstract":". Nowadays, researching of steroidal saponins (SE) is not present in Ecuador, a country where there is a less the science investment; in spite of owning endemic flora unexplored like Agave americana subps. andina (or the famous “penco azul”); it is a great specie for phytochemical and biotechnician studies. On the other hand, China has been the major SE productor since the end of twentieth century, and actuality, it progresses in prodrugs enhance. For this reason, the author of this short-paper explains how Ecuadorian biotechnicians can do an experimental design with a novel method of enzymatic hydrolysis. This innovative procedure uses a potato-dextrose subtract, which is a culture medium for yeasts of chaguarmishki. This method is useful for extracting O-glucuronidase, an enzyme that catalyze O-glycosides. These molecules are the active agent on Agave genus. Finally, the importance of this research is not for the advantages of SE on pharmaceutics or its human body effect; really, the principal benefit is the enhance of Ecuadorian pharmaceutical industry through a mass production with an investment on science investigation.","PeriodicalId":337320,"journal":{"name":"Proceedings of MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125646360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-02DOI: 10.3390/mol2net-05-06384
Ana Claudia Samaniego Villacis, Mauro Berta Ramasko, Laura Richmond, A. West
Blood disorders like B-thalassemia or sickle cell anaemia obligate patients to require constant blood transfusions. These, among others, make blood a highly demanded resource in the medical field. Blood has been obtained from in-vitro erythroid differentiation from embryonic stem cells or induced pluripotent stem cells, but this blood tends to produce embryonic haemoglobin. In this project, we aim to use a CRISPR/Cas9 modified version and an aptamer approach to alter the expression of the β-globin locus upregulating the gamma globin gene to produce foetal haemoglobin. Guide vectors were created targeting previously known sequences in the gamma haemoglobin gene. HEK cells were transfected with different combinations of CRISPR/Cas9 elements, as well as different combinations of aptamers/effectors, to test the amount of gene activation achieved. Gene expression analysis was performed through RTq/PCR. It was found that HbG activation can be achieved to some extent, however, the levels of gamma haemoglobin attained are very low compared with the normal levels of HbG expressed in other cell lines like K562. From the different grouping of vectors (dCas9 protein + aptamer/coating protein + activation effectors) tested, the combination of dCas9ES + PP7/PCP + VPH presented the best performance. References Basak, Anindita, and Vijay G. Sankaran. 2016. “Regulation of the Fetal Hemoglobin Silencing Factor BCL11A.” Annals of the New York Academy of Sciences 1368 (1): 25–30. doi:10.1111/nyas.13024. Borg, Joseph, Petros Papadopoulos, Marianthi Georgitsi, Laura Gutiérrez, Godfrey Grech, Pavlos Fanis, Marios Phylactides, et al. 2010. “Haploinsufficiency for the Erythroid Transcription Factor KLF1 Causes Hereditary Persistence of Fetal Hemoglobin.” Nature Genetics 42 (9): 801–5. doi:10.1038/ng.630. MOL2NET, 2019, 5, ISSN: 2624-5078 2 http://sciforum.net/conference/mol2net-05 Chavez, Alejandro, Marcelle Tuttle, Benjamin W. Pruitt, Ben Ewen-Campen, Raj Chari, Dmitry TerOvanesyan, Sabina J. Haque, et al. 2016. “Comparison of Cas9 Activators in Multiple Species.” Nature Methods 13 (7): 563–67. doi:10.1038/nmeth.3871. Chen, Baohui, Luke A. Gilbert, Beth A. Cimini, Joerg Schnitzbauer, Wei Zhang, Gene-Wei Li, Jason Park, et al. 2013. “Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System.” Cell 155 (7): 1479–91. doi:10.1016/j.cell.2013.12.001. Dominguez, Antonia A., Wendell A. Lim, and Lei S. Qi. 2016. “Beyond Editing: Repurposing CRISPR–Cas9 for Precision Genome Regulation and Interrogation.” Nature Reviews. Molecular Cell Biology 17 (1): 5–15. doi:10.1038/nrm.2015.2. Forget, Bernard G., and H. Franklin Bunn. 2013. “Classification of the Disorders of Hemoglobin.” Cold Spring Harbor Perspectives in Medicine 3 (2). doi:10.1101/cshperspect.a011684. Gilbert, Luke A., Matthew H. Larson, Leonardo Morsut, Zairan Liu, Gloria A. Brar, Sandra E. Torres, Noam Stern-Ginossar, et al. 2013. “CRISPR-Mediated Modular RNA-Guided Regulation of Transcripti
哺乳动物基因组:国际哺乳动物基因组学会官方杂志26(9-10):501-10。doi: 10.1007 / s00335 - 015 - 9565 - z。徐健，Vijay G. Sankaran, minni, Tobias F. Menne, Rishi V. Puram, Woojin Kim, Stuart H. Orkin。2010。“BCL11A对γ-球蛋白的转录沉默涉及与SOX6的远程相互作用和合作。”基因工程学报，24(8):783 - 798。doi: 10.1101 / gad.1897310。
{"title":"In vitro CRISPR-mediated gamma globin gene activation in HEK293","authors":"Ana Claudia Samaniego Villacis, Mauro Berta Ramasko, Laura Richmond, A. West","doi":"10.3390/mol2net-05-06384","DOIUrl":"https://doi.org/10.3390/mol2net-05-06384","url":null,"abstract":"Blood disorders like B-thalassemia or sickle cell anaemia obligate patients to require constant blood transfusions. These, among others, make blood a highly demanded resource in the medical field. Blood has been obtained from in-vitro erythroid differentiation from embryonic stem cells or induced pluripotent stem cells, but this blood tends to produce embryonic haemoglobin. In this project, we aim to use a CRISPR/Cas9 modified version and an aptamer approach to alter the expression of the β-globin locus upregulating the gamma globin gene to produce foetal haemoglobin. Guide vectors were created targeting previously known sequences in the gamma haemoglobin gene. HEK cells were transfected with different combinations of CRISPR/Cas9 elements, as well as different combinations of aptamers/effectors, to test the amount of gene activation achieved. Gene expression analysis was performed through RTq/PCR. It was found that HbG activation can be achieved to some extent, however, the levels of gamma haemoglobin attained are very low compared with the normal levels of HbG expressed in other cell lines like K562. From the different grouping of vectors (dCas9 protein + aptamer/coating protein + activation effectors) tested, the combination of dCas9ES + PP7/PCP + VPH presented the best performance. References Basak, Anindita, and Vijay G. Sankaran. 2016. “Regulation of the Fetal Hemoglobin Silencing Factor BCL11A.” Annals of the New York Academy of Sciences 1368 (1): 25–30. doi:10.1111/nyas.13024. Borg, Joseph, Petros Papadopoulos, Marianthi Georgitsi, Laura Gutiérrez, Godfrey Grech, Pavlos Fanis, Marios Phylactides, et al. 2010. “Haploinsufficiency for the Erythroid Transcription Factor KLF1 Causes Hereditary Persistence of Fetal Hemoglobin.” Nature Genetics 42 (9): 801–5. doi:10.1038/ng.630. MOL2NET, 2019, 5, ISSN: 2624-5078 2 http://sciforum.net/conference/mol2net-05 Chavez, Alejandro, Marcelle Tuttle, Benjamin W. Pruitt, Ben Ewen-Campen, Raj Chari, Dmitry TerOvanesyan, Sabina J. Haque, et al. 2016. “Comparison of Cas9 Activators in Multiple Species.” Nature Methods 13 (7): 563–67. doi:10.1038/nmeth.3871. Chen, Baohui, Luke A. Gilbert, Beth A. Cimini, Joerg Schnitzbauer, Wei Zhang, Gene-Wei Li, Jason Park, et al. 2013. “Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System.” Cell 155 (7): 1479–91. doi:10.1016/j.cell.2013.12.001. Dominguez, Antonia A., Wendell A. Lim, and Lei S. Qi. 2016. “Beyond Editing: Repurposing CRISPR–Cas9 for Precision Genome Regulation and Interrogation.” Nature Reviews. Molecular Cell Biology 17 (1): 5–15. doi:10.1038/nrm.2015.2. Forget, Bernard G., and H. Franklin Bunn. 2013. “Classification of the Disorders of Hemoglobin.” Cold Spring Harbor Perspectives in Medicine 3 (2). doi:10.1101/cshperspect.a011684. Gilbert, Luke A., Matthew H. Larson, Leonardo Morsut, Zairan Liu, Gloria A. Brar, Sandra E. Torres, Noam Stern-Ginossar, et al. 2013. “CRISPR-Mediated Modular RNA-Guided Regulation of Transcripti","PeriodicalId":337320,"journal":{"name":"Proceedings of MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition","volume":"90 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127582473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.3390/mol2net-05-06375
Favian Bayas Morejón, Riveliño Ramón Curay, Israel Goyes, D. Nuñez, K. Beltrán
{"title":"Fluoroquinolonas susceptibility analysis of arcobacter spp isolated of fresh cheese, from the city of guaranda","authors":"Favian Bayas Morejón, Riveliño Ramón Curay, Israel Goyes, D. Nuñez, K. Beltrán","doi":"10.3390/mol2net-05-06375","DOIUrl":"https://doi.org/10.3390/mol2net-05-06375","url":null,"abstract":"","PeriodicalId":337320,"journal":{"name":"Proceedings of MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition","volume":"6 7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123733347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.3390/mol2net-05-06368
T. Guerrero, S. Zapata
. Abstract Bacterial resistance to antibiotics is an emerging problem worldwide; antimicrobials used in animal husbandry are the most important factor in the propagation of resistance genes, because the amount of antibiotics used in the animal industry is greater than in the medical field. Since phages constitute a safe alternative for the control of human pathogenic bacteria in food, this project aimed to isolate and characterize Salmonella spp. bacteriophages and use them to reduce the presence of this pathogen in poultry at the farm level, without the use of antibiotics. We obtained phage cocktails that showed lytic activity in vitro against 83% of the Salmonella strains isolated from poultry farms. The cocktails with the greatest spectrum of activity against Salmonella were obtained from nearby polluted rivers. We were able to eliminate the pathogen from the chicken intestine when phages were applied 48 hours before the animals were slaughtered, but it is necessary to maintain a permanent monitoring of the phage's lytic activity against resistant strains of Salmonella.
{"title":"Bacteriophages as an alternative to the use of antibiotics in poultry breeding","authors":"T. Guerrero, S. Zapata","doi":"10.3390/mol2net-05-06368","DOIUrl":"https://doi.org/10.3390/mol2net-05-06368","url":null,"abstract":". Abstract Bacterial resistance to antibiotics is an emerging problem worldwide; antimicrobials used in animal husbandry are the most important factor in the propagation of resistance genes, because the amount of antibiotics used in the animal industry is greater than in the medical field. Since phages constitute a safe alternative for the control of human pathogenic bacteria in food, this project aimed to isolate and characterize Salmonella spp. bacteriophages and use them to reduce the presence of this pathogen in poultry at the farm level, without the use of antibiotics. We obtained phage cocktails that showed lytic activity in vitro against 83% of the Salmonella strains isolated from poultry farms. The cocktails with the greatest spectrum of activity against Salmonella were obtained from nearby polluted rivers. We were able to eliminate the pathogen from the chicken intestine when phages were applied 48 hours before the animals were slaughtered, but it is necessary to maintain a permanent monitoring of the phage's lytic activity against resistant strains of Salmonella.","PeriodicalId":337320,"journal":{"name":"Proceedings of MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition","volume":"144 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121299519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.3390/mol2net-05-06369
G. Méndez, L. Morales
{"title":"Carbamazepine degradation with isolated bacterias of Machángara river in Quito - Ecuador.","authors":"G. Méndez, L. Morales","doi":"10.3390/mol2net-05-06369","DOIUrl":"https://doi.org/10.3390/mol2net-05-06369","url":null,"abstract":"","PeriodicalId":337320,"journal":{"name":"Proceedings of MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124882321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-11-01DOI: 10.3390/mol2net-05-06376
Anuraj Nayarisseri, Ravina Khandelwal
Cancer stands as major cause of mortality in the world, and it's the morbidity has significantly increased in both developing and developed nations. In spite of the recent advancement in cancer therapies, the clinical follow-up still lags far behind.Recent studies show that, stem cells are bestowed with distinctive functions, like tumor cells relocation, immunosuppression and production of bioactive elements, that helps in cancer targeting that bypassobstacles.Recent understanding show that Preclinical stem cell-based strategies has proved potential for targeted anti-tumor therapy applications. Stem cell applications in modulation and remodeling of immune system happens to be frequent procedure used past ten years in successfully treating tumor.Generation of human somatic cells into induced pluripotent stem cells (iPSCs) has often been a time consuming laborious intensive and expensive process. Additionally, the major problem with iPSCs is their tendency to revert to original somatic state. Hence, a robust computational model in discovering genes/molecules necessary for iPSC generation and maintenance can be a major leap towards in stem cell research.The synergistic combination of genetic relationship data, advanced computing hardware and nonlinear algorithms and could make artificially-induced pluripotent stem cells (aiPSC) a near future reality. Genes or proteins that are known to be essential in human pluripotent stem cells (hPSC) could possibly be used for system modelling. The present investigation is aimed to develop an unsupervised deep machine learning technology for the prediction of genes relevant in aiPSC production and its maintenance for both common and rare diseases making it a cost-effective approach.
{"title":"A Machine learning approach for the prediction of efficient iPSC modeling","authors":"Anuraj Nayarisseri, Ravina Khandelwal","doi":"10.3390/mol2net-05-06376","DOIUrl":"https://doi.org/10.3390/mol2net-05-06376","url":null,"abstract":"Cancer stands as major cause of mortality in the world, and it's the morbidity has significantly increased in both developing and developed nations. In spite of the recent advancement in cancer therapies, the clinical follow-up still lags far behind.Recent studies show that, stem cells are bestowed with distinctive functions, like tumor cells relocation, immunosuppression and production of bioactive elements, that helps in cancer targeting that bypassobstacles.Recent understanding show that Preclinical stem cell-based strategies has proved potential for targeted anti-tumor therapy applications. Stem cell applications in modulation and remodeling of immune system happens to be frequent procedure used past ten years in successfully treating tumor.Generation of human somatic cells into induced pluripotent stem cells (iPSCs) has often been a time consuming laborious intensive and expensive process. Additionally, the major problem with iPSCs is their tendency to revert to original somatic state. Hence, a robust computational model in discovering genes/molecules necessary for iPSC generation and maintenance can be a major leap towards in stem cell research.The synergistic combination of genetic relationship data, advanced computing hardware and nonlinear algorithms and could make artificially-induced pluripotent stem cells (aiPSC) a near future reality. Genes or proteins that are known to be essential in human pluripotent stem cells (hPSC) could possibly be used for system modelling. The present investigation is aimed to develop an unsupervised deep machine learning technology for the prediction of genes relevant in aiPSC production and its maintenance for both common and rare diseases making it a cost-effective approach.","PeriodicalId":337320,"journal":{"name":"Proceedings of MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128742333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-24DOI: 10.3390/mol2net-05-06273
Luis A. Torres-Gómez, Dayrenis Garcia, J. Polo, L. Machin
The main objective of this study was to develop quantitative structure-activity relationships (QSAR) for the classification and prediction of anti-inflammatory activity. To this end, the ToSS-MoDE approximation was applied for the calculation of the spectral moments of the adjacency matrix between edges of the molecular graph with suppressed hydrogens, weighted on the main diagonal with moments of bond dipoles, bond distance, Van der Waals radius, polarizability and hydrophobicity to 509 active and inactive compounds. The calculated descriptors were used in the design of a training series and a prediction series. With the training series, a discriminant function was developed for the anti-inflammatory activity and another function to characterize the potential of these drugs using the Multivariate Linear Discriminant analysis, obtaining a good total classification of 96.07%. The model was validated by using the external prediction series, obtaining a good classification of 92.59%.
{"title":"Obtaining a computer-assisted QSAR model for the prediction of anti-inflammatory activity","authors":"Luis A. Torres-Gómez, Dayrenis Garcia, J. Polo, L. Machin","doi":"10.3390/mol2net-05-06273","DOIUrl":"https://doi.org/10.3390/mol2net-05-06273","url":null,"abstract":"The main objective of this study was to develop quantitative structure-activity relationships (QSAR) for the classification and prediction of anti-inflammatory activity. To this end, the ToSS-MoDE approximation was applied for the calculation of the spectral moments of the adjacency matrix between edges of the molecular graph with suppressed hydrogens, weighted on the main diagonal with moments of bond dipoles, bond distance, Van der Waals radius, polarizability and hydrophobicity to 509 active and inactive compounds. The calculated descriptors were used in the design of a training series and a prediction series. With the training series, a discriminant function was developed for the anti-inflammatory activity and another function to characterize the potential of these drugs using the Multivariate Linear Discriminant analysis, obtaining a good total classification of 96.07%. The model was validated by using the external prediction series, obtaining a good classification of 92.59%.","PeriodicalId":337320,"journal":{"name":"Proceedings of MOL2NET 2019, International Conference on Multidisciplinary Sciences, 5th edition","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116733007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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