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Cover Volume 14, No 2: Februari 2024 封面 第 14 卷第 2 期:2024 年 2 月
Pub Date : 2024-03-13 DOI: 10.24014/ja.v14i2.29163
Lutfi Arifin
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引用次数: 0
KARAKTERISASI MORFOLOGI DAN UJI ANTIFUNGI ISOLAT JAMUR Trichoderma spp. DARI TANAH GAMBUT TERHADAP PATOGEN PADA JARAK KEPYAR (Ricinus communis L.) CARACTERISASI MORFOLOGI DAN UJI ANTIFUNGI ISOLAT JAMUR Trichoderma spp. DARI TANAH GAMBUT TERHADAP PATOGEN PADA JARAK KEPYAR (Ricinus communis L.)
Pub Date : 2024-02-29 DOI: 10.24014/ja.v14i2.18479
Nurraodatul Aini, Atria Martina
Castor oil (Ricinus communis L.) is an oil producing commodities that plays a role in meeting industrial and economic needs. Infection with the pathogen fungi caused a decrease in the production of castor oil. Biological control using Trichoderma spp. has been widely used. Trichoderma spp. known as one of the benefical biofungicide because it has high antagonistic properties inhibiting the growth pathogenic fungi. The purpose of this study was to obtain isolates and characters as well antifungal test for Trichoderma spp. isolated from peat soil to control pathogen Aspergillus sp. on castor been (Ricinus communis L.). Trichoderma spp. isolated from peat soil in Meranti Islands, Riau. The antagonistic activity the isolates against pathogen Aspergillus sp. was studied in vitro using dual culture and in vivo assay. In this study, six isolates of Trichoderma spp have isolated. Trichoderma sp. GBA5 has the highest growth rate on PDA and TSM medium. Trichoderma sp. GBA3 and Trichoderma sp. GBA4 were most effective in percentage inhibition of mycelial growth of test pathogen i.e 84,55%  and 83,71% respectively. In in vivo assay, only Trichoderma sp. GBA5 inhibited pathogen with the percent infected castor capsule was 51.66%.
蓖麻油(Ricinus communis L.)是一种产油商品,在满足工业和经济需求方面发挥着重要作用。病原真菌感染导致蓖麻油产量下降。利用毛霉属真菌进行生物防治已得到广泛应用。毛霉属真菌被称为有益的生物杀真菌剂之一,因为它具有抑制病原真菌生长的高度拮抗特性。本研究的目的是从泥炭土中分离出毛霉菌属,对其进行分离、特征和抗真菌测试,以控制蓖麻(Ricinus communis L.)上的病原体曲霉菌属(Aspergillus sp.)。从廖内省默兰蒂群岛泥炭土中分离出的毛霉菌属(Trichoderma spp.通过双重培养和体内试验,研究了分离物对病原曲霉的拮抗活性。在这项研究中,共分离出 6 个毛霉属分离株。毛霉 GBA5 在 PDA 和 TSM 培养基上的生长率最高。Trichoderma sp. GBA3 和 Trichoderma sp. GBA4 对测试病原体菌丝生长的抑制率最高,分别为 84.55% 和 83.71%。在体内试验中,只有 GBA5 真菌对病原体有抑制作用,其感染蓖麻蒴果的百分比为 51.66%。
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引用次数: 0
HUBUNGAN POSISI APOKOL DALAM PERKECAMBAHAN AREN (Arenga pinnata Merr.) DENGAN PERTUMBUHAN KECAMBAH DAN KERAGAMAN GENETIK 桄榔(Arenga pinnata Merr.)播种中的果穗位置与萌芽生长和遗传多样性的关系
Pub Date : 2024-02-29 DOI: 10.24014/ja.v14i2.21882
Aswaldi Anwar, I. Dwipa, Dini Hervani, Afrima Sari
Sugar palm seed germination does indicate by the appearance of a tissue that extends like a sponge called apokol which is penetrating the hard seed coat and can occur in several positions in germinated sugar palm seeds. No research reports discuss the relationship between this apokol position and the phenology of sugar palm seed germination. This research aims to study the relationship between apokol position and the growth of sugar palm seedlings and their genetic diversity. The research applies Experiment in Completely Random Design with four treatments, each repeated five times. The four treatments are the apokol position, namely A: Apokol in right-center, B: Apokol in left-center, C: Apokol in left-bottom, and D: Apokol in right-bottom. The results showed that the fastest time for the coleoptile emergence was 40 days after transplanting in the right-center apokol position. Morphologically, there was no significant difference in the sugar palm seedling growth with each apokol position. On the other hand, we found that there were genetic diversities among them revealed by Simple Sequence Repeat (SSR) markers. The palm seeds with the right-center apokol position included the first cluster, and the second cluster consisted of left-center apokol, right- bottom apokol, and left-bottom apokol.
糖棕榈种子萌发时确实会出现一种像海绵一样延伸的组织,这种组织被称为apokol,它穿透坚硬的种皮,可以出现在萌发的糖棕榈种子的多个位置。目前还没有研究报告讨论这种apokol位置与糖棕榈种子萌发物候学之间的关系。本研究旨在研究apokol位置与糖棕榈幼苗生长及其遗传多样性之间的关系。研究采用完全随机设计试验,共有四个处理,每个处理重复五次。四种处理的凋落物位置分别为:A:凋落物在右中;B:凋落物在左中;C:凋落物在左下;D:凋落物在右下。结果表明,在右中心 Apokol 位置移植后 40 天,胚珠萌发最快。从形态上看,每个apokol位置的糖棕榈幼苗生长没有显著差异。另一方面,通过简单序列重复(SSR)标记,我们发现它们之间存在遗传多样性。第一群组包括右中apokol位置的棕榈种子,第二群组包括左中apokol、右下apokol和左下apokol。
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引用次数: 0
RESPON PERTUMBUHAN PROTOKORM ANGGREK SENDU (Grammatophyllum stapeliiflorum) DENGAN PENAMBAHAN BEBERAPA JENIS PUPUK DAUN SECARA IN VITRO 对 "茎叶藻类"(Grammatophyllum stapeliiflorum)的人工保护反应
Pub Date : 2024-02-29 DOI: 10.24014/ja.v14i2.17244
Mayta Novaliza Isda, Wasila Wusqa
Indonesia has 26,000 species of orchids that are very potential and profitable. Grammatophllum stapeliiflorum is an orchid species that is increasingly difficult to find in its natural habitat. The addition of foliar fertilizer containing macro and micro nutrients can increase plant growth. The purpose of this study was to determine the effect of foliar fertilizer type, concentration and the combination of the two factors on the growth of the orchid protocorm G. stapeliiflorum. This study used a factorial completely randomized design with two research factors, namely the type of foliar fertilizer with 2 treatment levels, B1 = Gandasil-D and B2 = Growmore. The second factor, fertilizer concentration with 4 treatment levels, namely A0 = control (without foliar fertilizer), A1 = 1 g/L, A2 = 2 g/L, and A3 = 3 g/L. This study consisted of 8 treatment combinations which were repeated 4 times, resulting in 32 experimental units. The parameters observed were the percentage of live explants, the percentage of browning, and the percentage of protocorms growth stages. Data were analyzed by Analysis of Variance (ANOVA) and continued with Duncan's Multiple Range Test (DMRT) at a 5% level. These results indicated that the application of foliar fertilizers with Gandasil-D and Growmore affects the percentage of live explants and protocorm growth phases and also increase the percentage browning until of 32,5%. Gandasil-D is better for protocorm growth from globular to torpedo. The control is better for forming shoot of 22,5% compared to the use of foliar fertilizer.
印度尼西亚有 26,000 种兰花,这些兰花潜力巨大,利润丰厚。Grammatophllum stapeliiflorum 是一种在自然栖息地越来越难找到的兰花品种。添加含有宏量和微量营养元素的叶面肥可以促进植物生长。本研究的目的是确定叶面肥的类型、浓度以及这两个因素的组合对兰花原茎 G. stapeliiflorum 生长的影响。本研究采用了完全随机的因子设计,其中有两个研究因子,即叶面肥的类型,有两个处理水平:B1 = Gandasil-D 和 B2 = Growmore。第二个因素是肥料浓度,有 4 个处理水平,即 A0 = 对照(无叶面肥)、A1 = 1 克/升、A2 = 2 克/升和 A3 = 3 克/升。这项研究包括 8 个处理组合,重复 4 次,共 32 个实验单元。观察参数包括活体外植体百分比、褐变百分比和原球茎生长阶段百分比。对数据进行了方差分析(ANOVA),并在 5%的水平上继续进行邓肯多重范围检验(DMRT)。这些结果表明,施用 Gandasil-D 和 Growmore 叶面肥会影响活体外植体和原球茎生长阶段的百分比,也会增加褐变的百分比,直到 32.5%。Gandasil-D 更适合原球茎从球状到鱼雷状的生长。与使用叶面肥相比,对照组更有利于形成 22.5%的新芽。
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引用次数: 0
EFEKTIVITAS BIOCHAR TERHADAP KETERSEDIAAN UNSUR HARA MIKRO PADA ULTISOL 生物炭对超微土壤中微量营养元素供应的影响
Pub Date : 2024-02-29 DOI: 10.24014/ja.v14i2.26686
Oksana Oksana, Irwan Taslapratama, Muhammad Ali Novia, Yusmar Mahmud, Tiara Septirosya, Raudhatu Shofiah
Biochar with a high organic matter composition is considered to be an ameliorant for Ultisol. Providing biochar as an ameliorant needs to be studied because it will cause an increase in micronutrients, most of which are excessive in Ultisol. This study aims to determine the effect of several levels of biochar dosage from corn cob as a base material on changes in micronutrients in Ultisol soil. This research was conducted in a greenhouse and in the Laboratory of Agrostology, Feed Industry and Soil Science, State Islamic University of Sultan Syarif Kasim Riau. Experiments with several doses of biochar as treatment, e.g., 0%, 5%, 10%, and 15% of the composition of the growing media were arranged in a Completely Randomized Design (CRD) with six replications. The parameters observed were the micronutrient content of Boron (B), Copper (Cu), Zinc (Zn), Iron (Fe) and Aluminum (Al). The experimental results showed that giving corn cob biochar with doses of 0.5%, 10% and 15% did not significantly change the levels of B, Cu and Zn Ultisol. Biochar at a dose of 10% significantly increased the soil Zn content and decreased Al-dd Ultisol solubility. 
高有机质成分的生物炭被认为是一种改善 Ultisol 条件的物质。将生物炭作为一种改良剂需要进行研究,因为它将导致微量营养元素的增加,而大多数微量营养元素在 Ultisol 中含量过高。本研究旨在确定以玉米芯为基质的生物炭的不同用量对 Ultisol 土壤中微量营养元素变化的影响。这项研究是在廖内苏丹-赛里夫-卡西姆国立伊斯兰大学的温室和农业学、饲料工业和土壤科学实验室进行的。实验采用完全随机设计法(CRD),将生物炭的不同剂量作为处理,如生长介质成分的 0%、5%、10% 和 15%,共六次重复。观察参数为硼(B)、铜(Cu)、锌(Zn)、铁(Fe)和铝(Al)的微量营养元素含量。实验结果表明,玉米芯生物炭的剂量为 0.5%、10% 和 15%,并没有显著改变硼、铜和锌的含量。剂量为 10%的生物炭明显增加了土壤中的锌含量,降低了 Al-dd Ultisol 的溶解度。
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引用次数: 0
PENGARUH WARNA CAHAYA TERHADAP MORFOGENESIS EKSPLAN KALUS BAWANG PUTIH (Allium sativum L.) SECARA IN-VITRO 光色对白玉米外植体(Allium sativum L.)体外繁殖的影响
Pub Date : 2024-02-29 DOI: 10.24014/ja.v14i2.25211
Sindi Ramadani, Nilla Kristina, Auzar Syarif, Elara Resigia
The quality of the Garlic seed bulbs needs to be improved by seed propagation through tissue culture techniques to get uniform and big cloves. Utilizing the suitable color spectrum of light is one way to stimulate plant morphogenesis. This study aimed to identify the influence of light color and obtain the best color in the morphogenesis of garlic callus explants. This research was carried out from June to September 2022 at the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Andalas. This study used a completely randomized design with three treatment levels: red, blue, and white. The F test analyzed observational data at a 5% level, followed by Duncan's multiple Range Test (DNMRT) at a 5% level. The results showed light color influences embryogenic callus formation and shoot formation from garlic callus explants. Blue light is better than red and white light in inducing embryogenic callus, increasing the rate of explants forming shoots but giving no significant difference in the length of the shoot.
大蒜种球的质量需要通过组织培养技术进行种子繁殖来提高,以获得均匀的大蒜瓣。利用合适的光色光谱是刺激植物形态发生的方法之一。本研究旨在确定光色对大蒜胼胝体外植体形态发生的影响,并获得最佳光色。这项研究于 2022 年 6 月至 9 月在安达卢西亚大学农学院组织培养实验室进行。该研究采用完全随机设计,有三个处理水平:红色、蓝色和白色。在 5%的水平上用 F 检验分析观察数据,然后在 5%的水平上用邓肯多重范围检验(DNMRT)分析观察数据。结果表明,光色对大蒜胼胝体外植体的胚胎胼胝形成和芽形成有影响。蓝光比红光和白光诱导胚化胼胝体的效果更好,能提高外植体形成芽的速度,但在芽的长度上没有显著差异。
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引用次数: 0
STUDI KARAKTERISTIK MORFOLOGI BEBERAPA VARIETAS TANAMAN SELADA (Lactuca sativa L.) HASIL INTRODUKSI 莴苣(Lactuca sativa L.)部分品种的形态特征研究 引言 结果
Pub Date : 2024-02-29 DOI: 10.24014/ja.v14i2.22476
Ade Sumiahadi, Yukarie Ayu Wulandari, Dirgahani Putri
Lettuce is a vegetable crop with a demand that continues to increase yearly. This situation is influenced by increasing public awareness of healthy lifestyles and urban farming practices. Diverse genetic sources are essential in plant breeding programs to form varieties with superior characteristics to increase productivity. One of the methods that can be done to improve genetic variation is by introduction. This research aims to study the morphological characteristics of three varieties of introduced lettuce. This research was conducted in the experimental field of the Faculty of Agriculture, Universitas Muhammadiyah Jakarta, from July to November 2022. The study compared three introduced lettuce varieties from Turkiye and three national commercial varieties. The morphological characteristics observed included qualitative (leaf shape, leaf vein shape, leaf tip shape, and leaf colour) and quantitative (crop height, number of leaves, leaf width, leaf length, harvest age, gross weight, and consumption weight). The results showed that only V4 had the same qualitative characteristics as its comparison variety (V1). The results also showed that in terms of quantitative characteristics, V4 and V5 had a higher resemblance to their comparator varieties (V1 and V2, respectively), while V6 had a further similarity to its comparator variety (V3). These introduced varieties have phenotypic variability that can be used as sources of plant genetics for plant breeding programs.
生菜是一种需求量逐年增加的蔬菜作物。公众对健康生活方式和城市耕作方式的认识不断提高,对这种情况产生了影响。在植物育种计划中,为了培育出具有优良特性的品种以提高产量,多样化的基因来源至关重要。引种是改善遗传变异的方法之一。本研究旨在研究三个引进莴苣品种的形态特征。该研究于 2022 年 7 月至 11 月在雅加达穆罕默迪亚大学(Universitas Muhammadiyah Jakarta)农学院的实验田进行。研究比较了三个从土耳其引进的莴苣品种和三个国内商业品种。观察到的形态特征包括定性特征(叶片形状、叶脉形状、叶尖形状和叶片颜色)和定量特征(作物高度、叶片数量、叶片宽度、叶片长度、收获年龄、毛重和消耗重量)。结果表明,只有 V4 的质量特性与对比品种(V1)相同。结果还显示,在数量特征方面,V4 和 V5 与参照品种(分别为 V1 和 V2)的相似度较高,而 V6 与参照品种(V3)的相似度更高。这些引进品种具有表型变异性,可作为植物育种计划的植物遗传资源。
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引用次数: 0
POTENTIAL EFFECT OF RHIZOBACTERIA Streptomyces sp. AND Bacillus sp. TO GEMINIVIRUS INFECTION AND RED CHILI (Capsicum annum L.) QUALITY PRODUCTION 链霉菌根杆菌和芽孢杆菌对双病毒感染和红辣椒的潜在影响质量生产
Pub Date : 2023-08-25 DOI: 10.24014/ja.v14i1.24689
Azizah Ridha Ulilalbab, S. Sulandari, T. Arwiyanto, S. Hartono
The chili yellow leaf curl disease is one of the biggest obctacle in chili’s production in Indonesia. This diseases only transmitted by whitefly (Bemisia tabaci). One of the disease controlling method is by using Plant Growth Promoting Rhizobacteria (PGPR) to reduce pesticide application for the health and environment. The aim of this research is to find out the effectiveness of Streptomyces sp. and Bacillus sp. in supressing yellow leaf curl disease, plant growth and chili harvest. This research has conducted at the experimental farmer’s field in Harjobinangun, Pakem, Sleman, Special Region of Yogyakarta. Using varieties of chili Twist 42, Streptomyces isolates which from Bacteriology Laboratory collection’s with 10-8 CFU/ml and Bacillus isolates which is from Mycrobiology Laboratory collection with  10-8 CFU/ml. The result of this research showed that the treatment of combination between Bacillus sp. and Streptomyces sp. could decrease and supress disease incidence and disease intensity if it compared with others treatment. Parameters of plant growth included plant high, stem diameter, root length, root volume and number of productive branch showed that single of Streptomyces sp. and Bacillus sp. without combination were better than combination and untreated.  The single application Streptomyces sp. or Bacillus sp. could increase amount of total number and weight of fruits.
辣椒黄卷叶病是印尼辣椒生产的最大障碍之一。本疾病仅由粉虱(烟粉虱)传播。利用植物促生根瘤菌(Plant Growth Promoting Rhizobacteria, PGPR)减少农药用量是防治病害的一种方法,有利于健康和环境。本研究的目的是了解链霉菌和芽孢杆菌对辣椒黄曲病、植株生长和收获的抑制效果。本研究在日惹特区Pakem、Sleman、Harjobinangun的试验田进行。利用麻花42辣椒品种,从细菌学实验室收集的链霉菌分离株为10-8 CFU/ml,从真菌学实验室收集的芽孢杆菌分离株为10-8 CFU/ml。本研究结果表明,与其他处理相比,芽孢杆菌与链霉菌联合处理可降低和抑制疾病发病率和疾病强度。株高、茎粗、根长、根体积和生产分枝数等生长指标表明,链霉菌与芽孢杆菌单株未组合的生长效果优于组合和未经处理的生长效果。单施链霉菌或芽孢杆菌可增加果实总数和重量。
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引用次数: 0
PENGARUH KONSENTRASI DAN LAMA PERENDAMAN ASAM SULFAT TERHADAP PERKECAMBAHAN BENIH DELIMA MERAH (Punica granatum L.) 聚合和长期浸泡硫酸对红豆发芽的影响(Punica granatum L)。
Pub Date : 2023-08-24 DOI: 10.24014/ja.v14i1.17481
Rita Elfianis, N. Putri, Syukria Ikhsan Zam
The problem of propagation of Punica granatum, was hard seed, and it difficult to germinate. One of the efforts to solve the problem is using H2SO4 as to breakdown of the hard seed. This study aimed to obtain the best concentration of H2SO4 and soaking time, as well as the interaction between H2SO4 concentration and soaking time on the germination of  Punica granatum seeds. This study used a completely randomized design (CRD) consisting of 2 factors. The first factor is H2SO4 concentration (0%, 20%, 40%, 60%, and 80%), and the second factor is soaking time (10, 15, and 20 minutes). Parameters observed were germination, germination rate, vigor index, germination height, root length, weight of sprouts, and dry weight of Punica granatum sprouts. The results showed the use of water was the best treatment on germination (85.30%), germination rate (17.37 days), vigor index (2.24%), germination height (7.80 cm), and root length of Punica granatum (7.05 cm), soaking time did not effect the germination of Punica granatum, and the interaction between the use of water and the soaking time 15 minutes was the best treatment on the parameters of fresh weight of sprouts (0.17 g) and dry weight of Punica granatum sprouts (0.13 g).
石榴的繁殖问题是种子坚硬,发芽困难。解决这个问题的方法之一是使用H2SO4来分解坚硬的种子。本研究旨在获得最佳H2SO4浓度和浸泡时间,以及H2SO4浓度和浸泡时间对石榴种子萌发的相互作用。本研究采用由2个因素组成的完全随机设计(CRD)。第一个影响因素是H2SO4浓度(0%、20%、40%、60%、80%),第二个影响因素是浸泡时间(10、15、20分钟)。观察了石榴芽的萌发率、发芽率、活力指数、萌发高度、根长、芽重和干重。结果表明:水处理对石榴芽的发芽率(85.30%)、发芽率(17.37 d)、活力指数(2.24%)、萌发高度(7.80 cm)和根长(7.05 cm)的影响最大,浸泡时间对石榴芽的萌发无影响,水处理与浸泡时间15 min的交互作用对石榴芽鲜重(0.17 g)和干重(0.13 g)的影响最大。
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引用次数: 0
PEMBERIAN FUNGI MIKORIZA ARBUSKULA DAN BIOCHAR TERHADAP PERTUMBUHAN DAN HASIL TANAMAN KEDELAI (Glycine max (L.) Merril) PADA TANAH ULTISOL 菌根菌和生物炭真菌用于大豆的生长和结果(gl)。在ULTISOL陆地上
Pub Date : 2023-08-24 DOI: 10.24014/ja.v14i1.16725
Sidik Yunedi, A. Perdana
Soybean is one of the main needs and sources of vegetable protein for Indonesian. National needs of  soybean continue to increase in line with the increase of population, but soybean production has not been able to meet national needs. Therefore, efforts need to be made to increase soybean production by utilizing marginal land with using arbuscular mycorrhizal fungi and biochar. The aim of this study was to determine the effect of single factors of arbuscular mycorrhizal fungi (AMF), biochar and interaction of these two factors on the growth and yield of soybean in Ultisol soil. This research was conducted from  December until March 2019. The research used 3 x 3 factorial completely randomized design with three replications. The first factor was AMF dosage consist of 3 levels : M0 (0 g per plant), M1 (15 g per plant), M2 (30 g per plant) and the second factor was type of biochar consist of 3 levels : B0 (without biochar), B1 (biochar of rice husk), B2 (biochar of coconut shell). The observation data that obtained were analyzed statistically using analysis of variance (ANOVA). The results of variance analysis were continued by Duncan's multiple range test at level 5%. The results showed that the treatment of AMF improved all growth and yield parameters and the single biochar can not improve all growth and yield parameters. The interaction between AMF and biochar did not improve all parameters of growth and yield of  soybean.
大豆是印尼植物蛋白的主要需求和来源之一。随着人口的增加,国家对大豆的需求也在不断增加,但大豆的产量一直不能满足国家的需求。因此,应努力利用边际土地,利用丛枝菌根真菌和生物炭提高大豆产量。本试验旨在研究丛枝菌根真菌(AMF)单因子、生物炭及其互作对Ultisol土壤中大豆生长和产量的影响。这项研究从12月到2019年3月进行。本研究采用3 × 3阶乘完全随机设计,3个重复。第一个因子是AMF用量,由M0 (0 g /株)、M1 (15 g /株)、M2 (30 g /株)3个水平组成;第二个因子是生物炭类型,由B0(不含生物炭)、B1(稻壳生物炭)、B2(椰壳生物炭)3个水平组成。获得的观察资料采用方差分析(ANOVA)进行统计学分析。方差分析结果在5%水平下继续进行Duncan多元极差检验。结果表明,AMF处理提高了所有生长和产量参数,而单一生物炭处理不能提高所有生长和产量参数。AMF与生物炭互作对大豆生长和产量的影响并不明显。
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引用次数: 0
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