Pub Date : 2022-06-15DOI: 10.58465/natprobiotech.2022.5
K. S. Kumar, I. Bezirganoglu, Büşra Yazıcılar, Merve Simsek Geyik, Fatma Ozge Aslan
Vicia sativa L. is one of most significant forages all over the world, but yield is decreased by cold stress. This study aimed to investigate the cold stress mechanism of V. sativaunder tissue culture in response to some biochemical analysis. In this study, six V. sativacultivars (Tarım Beyazı, Ankara Moru, 24 Çilli,Kansur and Aygün) were carried out to determine cold resistance in vitroconditions. Doğu Beyazı cultivar was used as a cold resistant plant. For the cold acclimation, two weeks old seedlings were incubated in the test chamber set at 4°C for 14 days to induce cold stress. Leaf samples were obtained at 14 days after cold treatment for physiological analysis evaluation. Cold resistant cultivars were chosen using values of thermal degrees. Moreover, antioxidant enzyme activities were determined at cold acclimated and non-acclimated seedlings. Peroxidase enzyme activities gradually increased at cold acclimation compared to those seedlings at non-acclimated.The highest POX activity was found at Ankara Moru seedlings, while the lowest activity was found at Aygün seedlings. SOD and APX activities were detected inversely at cold acclimation compared to those seedlings at non-acclimated. ChlorophyII A, chlorophyII B and carotenoid values were also determined at cold acclimated seedlings. The highest activities and pigment values were detected at Tarım Beyazı seedlings. Whereas, the lowest activities were found at Ankara Moru and Aygün cultivars. Our results displayed that cold acclimation linked to SOD, APX and POX activities and pigment estimation at in vitro conditions.
{"title":"The Impact of Cold Acclimatization on Antioxidant Enzyme Activity of Vicia sativa L.","authors":"K. S. Kumar, I. Bezirganoglu, Büşra Yazıcılar, Merve Simsek Geyik, Fatma Ozge Aslan","doi":"10.58465/natprobiotech.2022.5","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.5","url":null,"abstract":"Vicia sativa L. is one of most significant forages all over the world, but yield is decreased by cold stress. This study aimed to investigate the cold stress mechanism of V. sativaunder tissue culture in response to some biochemical analysis. In this study, six V. sativacultivars (Tarım Beyazı, Ankara Moru, 24 Çilli,Kansur and Aygün) were carried out to determine cold resistance in vitroconditions. Doğu Beyazı cultivar was used as a cold resistant plant. For the cold acclimation, two weeks old seedlings were incubated in the test chamber set at 4°C for 14 days to induce cold stress. Leaf samples were obtained at 14 days after cold treatment for physiological analysis evaluation. Cold resistant cultivars were chosen using values of thermal degrees. Moreover, antioxidant enzyme activities were determined at cold acclimated and non-acclimated seedlings. Peroxidase enzyme activities gradually increased at cold acclimation compared to those seedlings at non-acclimated.The highest POX activity was found at Ankara Moru seedlings, while the lowest activity was found at Aygün seedlings. SOD and APX activities were detected inversely at cold acclimation compared to those seedlings at non-acclimated. ChlorophyII A, chlorophyII B and carotenoid values were also determined at cold acclimated seedlings. The highest activities and pigment values were detected at Tarım Beyazı seedlings. Whereas, the lowest activities were found at Ankara Moru and Aygün cultivars. Our results displayed that cold acclimation linked to SOD, APX and POX activities and pigment estimation at in vitro conditions.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116088954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-15DOI: 10.58465/natprobiotech.2022.4
Canan Eroglu Gunes, Mücahit Seçme, E. Kurar, Huseyin Donmez
Pancreatic cancer is known to have one of the highest mortality rates among other cancer. Monoterpenes are natural compounds obtained from essential oils of plants. Carvacrol is a monoterpene phenolic derived from essential oils of various aromatic plants.In this study, the effects of carvacrol on proliferation, apoptosis and metastasis of PANC-1 human pancreatic cancer cells were investigated. XTT assay was used to determine cell proliferation and viability. mRNA level expression levels of BAX, BCL2, CASP3, CASP7, CASP8, CASP9, CYCS, FADD, FAS and P53 were evaluated for determining the effect of carvacrol on apoptosis by qRT-PCR analysis. In addition, gene expressions of CDH1, CDH2, TIMP1, TIMP2, TIMP3, ZEB1 and ZEB2 were analyzed to evaluate its effect onmetastasis. Carvacrol inhibited the PANC-1 pancreatic cancer cell proliferation in dose and time dependent manner. Carvacrol treatment induced apoptosis by changing the expressions of genes important in apoptosis. Moreover, it suppressed the metastasis by effecting CDH1, CDH2, TIMP2, TIMP3 and ZEB2 expressions. In conclusion, carvacrol showed apoptotic and anti-metastatic effects in PANC-1 human pancreatic cancer cells. It is thereby concluded that carvacrol may have a therapeutic potential in pancreatic cancer.
{"title":"Apoptotic and Anti-Metastatic Effect of Carvacrol in PANC-1 Human Pancreatic Cancer Cells","authors":"Canan Eroglu Gunes, Mücahit Seçme, E. Kurar, Huseyin Donmez","doi":"10.58465/natprobiotech.2022.4","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.4","url":null,"abstract":"Pancreatic cancer is known to have one of the highest mortality rates among other cancer. Monoterpenes are natural compounds obtained from essential oils of plants. Carvacrol is a monoterpene phenolic derived from essential oils of various aromatic plants.In this study, the effects of carvacrol on proliferation, apoptosis and metastasis of PANC-1 human pancreatic cancer cells were investigated. XTT assay was used to determine cell proliferation and viability. mRNA level expression levels of BAX, BCL2, CASP3, CASP7, CASP8, CASP9, CYCS, FADD, FAS and P53 were evaluated for determining the effect of carvacrol on apoptosis by qRT-PCR analysis. In addition, gene expressions of CDH1, CDH2, TIMP1, TIMP2, TIMP3, ZEB1 and ZEB2 were analyzed to evaluate its effect onmetastasis. Carvacrol inhibited the PANC-1 pancreatic cancer cell proliferation in dose and time dependent manner. Carvacrol treatment induced apoptosis by changing the expressions of genes important in apoptosis. Moreover, it suppressed the metastasis by effecting CDH1, CDH2, TIMP2, TIMP3 and ZEB2 expressions. In conclusion, carvacrol showed apoptotic and anti-metastatic effects in PANC-1 human pancreatic cancer cells. It is thereby concluded that carvacrol may have a therapeutic potential in pancreatic cancer.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132536042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-15DOI: 10.58465/natprobiotech.2022.6
Emel Yilmaz Gokdogan, B. Burun
Turkey has a rich flora in terms of plant biodiversity, and economically important Salviaspecies because of medicinal properties are in danger of extinction. To prevent the extinction of Salvia species is important to solvingthe problems of seed germination and micropropagation. In this review, in vitrostudies conducted on Salvia species from Turkish flora were listed by specifying danger categories. Seed germination studies carried out 18 Salvia species [S. candidissima Vahl., S. cyanescens Boiss. & Balansa (endemic/LR-lc), S. dichroantha Stapf (endemic/LR-lc), S. fruticosaMill. (VU), S. macrosiphon Boiss., S. microstegia Boiss. et Bal.,S. nemorosa L., S. officinalis L., S. pomifera ssp. pomiferaL. (VU), S. sclarea L., S. siirticaKahraman, Celep & Doğan sp. nov.(endemic/CR), S. smyrnaeaBoiss. (endemic/EN), S. tomentosa Mill., S. verbenaca L., S. verticillata L., S. virgata Jacq.,S. viridis L.]. In vitro culture studies were conducted on 16 Salvia species [S. aethiopis L.,S. albimaculata Hedge and Huber-Morath(endemic/VU)-unsuccessful result, S. cadmica Boiss. (endemic/LR-lc), S. candidissima Vahlssp. occidentalis Hedge,S. chrysophyllaStapf (endemic/LR-cd), S. cryptantha Montbret et Aucher ex Bentham(endemic/LR-lc), S. euphraticaMontbret et Aucher ex. Bentham var. euphratica(endemic/LR-cd)-unsuccessful result, S. fruticosa(VU), S. nemorosa, S. nydeggeri Hub.-Mor.(endemic/EN)-unsuccessful result, S. officinalis, S. sclarea, S. tomentosa, S. verticillata ssp. verticillata, S. virgata,S. viridis].In this review has been reported up to now in vitro cultures have been carried out 26 of 99 Salviaspecies, has clearly shows that new protocols should be established to protect endemic and threatened Salvia species.
{"title":"The Studies on Seed Germination and in Vitro Cultures of Salvia L.Species from Turkish Flora","authors":"Emel Yilmaz Gokdogan, B. Burun","doi":"10.58465/natprobiotech.2022.6","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.6","url":null,"abstract":"Turkey has a rich flora in terms of plant biodiversity, and economically important Salviaspecies because of medicinal properties are in danger of extinction. To prevent the extinction of Salvia species is important to solvingthe problems of seed germination and micropropagation. In this review, in vitrostudies conducted on Salvia species from Turkish flora were listed by specifying danger categories. Seed germination studies carried out 18 Salvia species [S. candidissima Vahl., S. cyanescens Boiss. & Balansa (endemic/LR-lc), S. dichroantha Stapf (endemic/LR-lc), S. fruticosaMill. (VU), S. macrosiphon Boiss., S. microstegia Boiss. et Bal.,S. nemorosa L., S. officinalis L., S. pomifera ssp. pomiferaL. (VU), S. sclarea L., S. siirticaKahraman, Celep & Doğan sp. nov.(endemic/CR), S. smyrnaeaBoiss. (endemic/EN), S. tomentosa Mill., S. verbenaca L., S. verticillata L., S. virgata Jacq.,S. viridis L.]. In vitro culture studies were conducted on 16 Salvia species [S. aethiopis L.,S. albimaculata Hedge and Huber-Morath(endemic/VU)-unsuccessful result, S. cadmica Boiss. (endemic/LR-lc), S. candidissima Vahlssp. occidentalis Hedge,S. chrysophyllaStapf (endemic/LR-cd), S. cryptantha Montbret et Aucher ex Bentham(endemic/LR-lc), S. euphraticaMontbret et Aucher ex. Bentham var. euphratica(endemic/LR-cd)-unsuccessful result, S. fruticosa(VU), S. nemorosa, S. nydeggeri Hub.-Mor.(endemic/EN)-unsuccessful result, S. officinalis, S. sclarea, S. tomentosa, S. verticillata ssp. verticillata, S. virgata,S. viridis].In this review has been reported up to now in vitro cultures have been carried out 26 of 99 Salviaspecies, has clearly shows that new protocols should be established to protect endemic and threatened Salvia species.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114687749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-05DOI: 10.58465/natprobiotech.2022.3
Burak Muhammed Oner, E. Ilhan, Ayşe Gül Kasapoğlu, Selman Muslu, Ahmed Sidar Aygoren, Sumeyra Ucar, E. Yaprak, Murat Isiyel, Recep Aydinyurt, M. Aydın
Nonexpressor of pathogenesis-related genes 1 (NPR1) could be found in the regulationof pathways of salicylic acid (SA) signal transduction and systemic acquired resistance (SAR) in plants. It has been found that the members of NPR-like gene family are interconnectingwith biotic and abiotic stresses in several plants, but it has not yet been performed in common bean. In this study, in silicoanalyzes of Phaseolus vulgaris L. NPR-likegene family members were performed at the genome level. As a result of these in silico analyzes, four NPR1genes were identified and it was found that these genes have been located on four different P. vulgarischromosomes. The length of PvNPR-like proteins ranges from 475-590 amino acid residues. The theoretical isoelectric point (pI) range were found between 5.75-6.36 and all show acidic properties. As a result of phylogenetic analyzes performed between NPR-like genes of P. vulgaris, Glycine max(L.) Merr and Arabidopsis thaliana(L.) Heynh. plants, NPR-likegenes were found to be divided into three different groups (A, B and C). It has been determined that PvNPR-likegenes contain cis-actingelements that function in many biological processes, including stress and hormone responses. Differences in gene expression levels under salt and drought stress indicated that PvNPR-likegenes respond differently under stress conditions. The outcomes of this studywill supply the needed basics for understanding the function and evolutionary history of PvNPR-like genes.
{"title":"Genome Wide Analysis and Characterization of NPR-likeGene Family of Phaseolus vulgaris L.","authors":"Burak Muhammed Oner, E. Ilhan, Ayşe Gül Kasapoğlu, Selman Muslu, Ahmed Sidar Aygoren, Sumeyra Ucar, E. Yaprak, Murat Isiyel, Recep Aydinyurt, M. Aydın","doi":"10.58465/natprobiotech.2022.3","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.3","url":null,"abstract":"Nonexpressor of pathogenesis-related genes 1 (NPR1) could be found in the regulationof pathways of salicylic acid (SA) signal transduction and systemic acquired resistance (SAR) in plants. It has been found that the members of NPR-like gene family are interconnectingwith biotic and abiotic stresses in several plants, but it has not yet been performed in common bean. In this study, in silicoanalyzes of Phaseolus vulgaris L. NPR-likegene family members were performed at the genome level. As a result of these in silico analyzes, four NPR1genes were identified and it was found that these genes have been located on four different P. vulgarischromosomes. The length of PvNPR-like proteins ranges from 475-590 amino acid residues. The theoretical isoelectric point (pI) range were found between 5.75-6.36 and all show acidic properties. As a result of phylogenetic analyzes performed between NPR-like genes of P. vulgaris, Glycine max(L.) Merr and Arabidopsis thaliana(L.) Heynh. plants, NPR-likegenes were found to be divided into three different groups (A, B and C). It has been determined that PvNPR-likegenes contain cis-actingelements that function in many biological processes, including stress and hormone responses. Differences in gene expression levels under salt and drought stress indicated that PvNPR-likegenes respond differently under stress conditions. The outcomes of this studywill supply the needed basics for understanding the function and evolutionary history of PvNPR-like genes.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126534109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-20DOI: 10.58465/natprobiotech.2022.2
Esra Yildirim Servi, Hüseyin Servi
Medicinal plants have been preferred sources against drug-resistant pathogens recently. The antimicrobial activities of essential oils have been known for a long time. In the present research, the chemical composition and antibacterial activity of commercial essential oil of Helichrysum italicum (Roth) G. Don (HIE) were evaluated. Seventy components which constitute 95.7%, were identified in the oil. The major constituents of HIE were neryl acetate (15.6%), α-pinene (11.5%), and γ-curcumene (11.3%). The oilwas screened for antibacterial activity by agar well diffusion and broth microdilution methods. The essential oil displayed remarkable antibacterial activity against test bacteria, except for Escherichia coli, which displayed resistance. The minimum inhibitory concentration (MIC) of essential oil were 5 μg/mL for Staphylococcus aureus and Pseudomonas aeruginosa, 10 μg/mL for Bacillus cereus and B. subtilis. These results suggested that the commercial oil of H. italicum has the potential to be used as a natural antibacterial agent.
近年来药用植物已成为抗耐药病原菌的首选来源。精油的抗菌作用早已为人所知。本研究对意大利蜡菊(Helichrysum italicum, Roth) G. Don, HIE)商品精油的化学成分和抑菌活性进行了评价。在油中鉴定出70种成分，占95.7%。HIE的主要成分为醋酸橙酯(15.6%)、α-蒎烯(11.5%)和γ-姜黄烯(11.3%)。采用琼脂孔扩散法和肉汤微量稀释法对其抑菌活性进行了筛选。除大肠杆菌耐药外，精油对其他细菌均有明显的抑菌活性。精油对金黄色葡萄球菌和铜绿假单胞菌的最低抑菌浓度为5 μg/mL，对蜡样芽孢杆菌和枯草芽孢杆菌的最低抑菌浓度为10 μg/mL。这些结果表明，意大利莲商品油具有作为天然抗菌剂的潜力。
{"title":"Chemical Composition and Antibacterial Activity of Helichrysum italicum (Roth) G. Don Commercial Essential Oil from Serbia","authors":"Esra Yildirim Servi, Hüseyin Servi","doi":"10.58465/natprobiotech.2022.2","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.2","url":null,"abstract":"Medicinal plants have been preferred sources against drug-resistant pathogens recently. The antimicrobial activities of essential oils have been known for a long time. In the present research, the chemical composition and antibacterial activity of commercial essential oil of Helichrysum italicum (Roth) G. Don (HIE) were evaluated. Seventy components which constitute 95.7%, were identified in the oil. The major constituents of HIE were neryl acetate (15.6%), α-pinene (11.5%), and γ-curcumene (11.3%). The oilwas screened for antibacterial activity by agar well diffusion and broth microdilution methods. The essential oil displayed remarkable antibacterial activity against test bacteria, except for Escherichia coli, which displayed resistance. The minimum inhibitory concentration (MIC) of essential oil were 5 μg/mL for Staphylococcus aureus and Pseudomonas aeruginosa, 10 μg/mL for Bacillus cereus and B. subtilis. These results suggested that the commercial oil of H. italicum has the potential to be used as a natural antibacterial agent.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"46 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131030818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-05-15DOI: 10.58465/natprobiotech.2022.1
Orhan Dincay, H. Civelek
As a result of the unconscious use of pesticides, honeybees are adversely affected, thus causing colony losses all over the world. In this research, different extraction and clean-up methods were compared on bee samples based on the QuEChERS method which is the most widely used pesticide analysis method in food. This research includes application studies for the multi residue method (MRM), which allows analysis of more than one pesticide. During the extraction phase, 7 different methods were compared, while the method with the most suitable repeatability and recovery values among these methods was taken to the clean-up phase. In the clean-up phase, 5 different clean-up methods were tried on the method which yielded the best values in extraction. In terms of average percentage recovery values, the 1stextraction method ranks first with 72 active substances between 70-120%, and the 3rd clean-up method with 92 active substances. Thus, while the number of active substances with suitable recovery values in the selected extraction method was 72, it increased to 92 with the continuation of the clean-up method, achieving a success rate of 92%. It is recommended to use this method in pesticide residue analyzes in bees with its outstanding features such as completing the analyzes in a short time, working easily with the infrastructure that can be found in all control laboratories, low analysis costs, ease of use, and reliability.
{"title":"Comparison of Extraction and Clean-up Methods Modified in Different Ways for the Determination of Pesticide Residues in Bees","authors":"Orhan Dincay, H. Civelek","doi":"10.58465/natprobiotech.2022.1","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.1","url":null,"abstract":"As a result of the unconscious use of pesticides, honeybees are adversely affected, thus causing colony losses all over the world. In this research, different extraction and clean-up methods were compared on bee samples based on the QuEChERS method which is the most widely used pesticide analysis method in food. This research includes application studies for the multi residue method (MRM), which allows analysis of more than one pesticide. During the extraction phase, 7 different methods were compared, while the method with the most suitable repeatability and recovery values among these methods was taken to the clean-up phase. In the clean-up phase, 5 different clean-up methods were tried on the method which yielded the best values in extraction. In terms of average percentage recovery values, the 1stextraction method ranks first with 72 active substances between 70-120%, and the 3rd clean-up method with 92 active substances. Thus, while the number of active substances with suitable recovery values in the selected extraction method was 72, it increased to 92 with the continuation of the clean-up method, achieving a success rate of 92%. It is recommended to use this method in pesticide residue analyzes in bees with its outstanding features such as completing the analyzes in a short time, working easily with the infrastructure that can be found in all control laboratories, low analysis costs, ease of use, and reliability.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128731189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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