Pub Date : 2023-06-15DOI: 10.58465/natprobiotech.2023.02
V. Mursaliyeva, Balaussa Sarsenbek, T. Mukhanov, Akjunus Imanbayeva
Malacocarpus crithmifolius is an endangered rare relict with small size populations grown in a limited area. The purpose of this study was to initiate in vitro and micro replication of Malacocarpus crithmifolius. The vegetative and reproductive explants and Murashige & Skoog medium (MS) supplemented with different concentrations and combinations of cytokinin and auxins were used. The response reaction of different explants and the plant growth regulators' effects on shooting frequency, the number of shoots, callusogenesis, and rooting percent were investigated. Culturing generative explants on the MS medium containing 6-Benzylaminopurine (BAP) combined with indole-3-acetic acid (IAA) or indole-butyric acid (IBA) led to direct shoot regeneration. The bud mass appeared in the leaf axil under inflorescences after one week of cultivation on the initiated medium. The replication was reached by micro-cutting of induced additional shoots and 3-fold passaging them on the fresh mediums. The average multiplication result at MS with BAP in combination with 1-Naphthaleneacetic acid (NAA) or IAA composed 5.85 after three months of cultivation. In conclusion, the applicable explant and phytohormonal composition of nutrient medium for initiation and micropropagation of M. crithmifolius were developed.
孔雀草（Malacocarpus crithmifolius）是一种濒危的稀有孑遗植物，其种群规模较小，生长在有限的区域内。本研究的目的是启动 Malacocarpus crithmifolius 的体外和微型复制。研究使用了无性和生殖外植体以及添加了不同浓度和组合的细胞分裂素和辅助素的 Murashige & Skoog 培养基（MS）。研究了不同外植体的反应以及植物生长调节剂对抽薹频率、抽薹数量、胼胝质发生和生根率的影响。在含有 6-苄基氨基嘌呤（BAP）和吲哚-3-乙酸（IAA）或吲哚-丁酸（IBA）的 MS 培养基上培养的外植体可直接产生新芽。在启动培养基上培养一周后，花序下的叶腋中出现芽块。将诱导出的新芽微切后，在新鲜培养基上进行 3 倍传代，即可实现复制。在 MS 上用 BAP 与 1-萘乙酸（NAA）或 IAA 结合，经过三个月的培养，平均繁殖结果为 5.85。总之，该研究开发出了适用于 M. crithmifolius 萌发和微繁殖的外植体和营养培养基的植物激素成分。
{"title":"Conservation in Tissue Culture of Malacocarpus crithmifolius (Retz.) Fisch. C.A.Mey. - Relict Species from Mangyshlak","authors":"V. Mursaliyeva, Balaussa Sarsenbek, T. Mukhanov, Akjunus Imanbayeva","doi":"10.58465/natprobiotech.2023.02","DOIUrl":"https://doi.org/10.58465/natprobiotech.2023.02","url":null,"abstract":"Malacocarpus crithmifolius is an endangered rare relict with small size populations grown in a limited area. The purpose of this study was to initiate in vitro and micro replication of Malacocarpus crithmifolius. The vegetative and reproductive explants and Murashige & Skoog medium (MS) supplemented with different concentrations and combinations of cytokinin and auxins were used. The response reaction of different explants and the plant growth regulators' effects on shooting frequency, the number of shoots, callusogenesis, and rooting percent were investigated. Culturing generative explants on the MS medium containing 6-Benzylaminopurine (BAP) combined with indole-3-acetic acid (IAA) or indole-butyric acid (IBA) led to direct shoot regeneration. The bud mass appeared in the leaf axil under inflorescences after one week of cultivation on the initiated medium. The replication was reached by micro-cutting of induced additional shoots and 3-fold passaging them on the fresh mediums. The average multiplication result at MS with BAP in combination with 1-Naphthaleneacetic acid (NAA) or IAA composed 5.85 after three months of cultivation. In conclusion, the applicable explant and phytohormonal composition of nutrient medium for initiation and micropropagation of M. crithmifolius were developed.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139369702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-15DOI: 10.58465/natprobiotech.2023.04
R. Marzban, G. B. Eroglu
Bacterial entomopathogens, especially Bacillus thuringiensis (Bt), are the most popular entomopathogen in bacteria worldwide, thanks to their toxin genes and high virulence. In this context, to develop an alternative to chemical pesticides used to control agricultural and forest pest insects, Bt has been isolated from soils and many insects, and products in the form of formulations have been prepared. It is also known that Bt-based biopesticides are generally more specific and harmless to other organisms. The results demonstrate that countries have different conditions for releasing these organic products to the market and it is often difficult for scientists to obtain a license. This study discusses the current status of bacterial entomopathogens study in Iran and Turkey.
{"title":"Status of Bacterial Entomopathogens Used for Microbial Control of Arthropod Pests in Iran and Turkey","authors":"R. Marzban, G. B. Eroglu","doi":"10.58465/natprobiotech.2023.04","DOIUrl":"https://doi.org/10.58465/natprobiotech.2023.04","url":null,"abstract":"Bacterial entomopathogens, especially Bacillus thuringiensis (Bt), are the most popular entomopathogen in bacteria worldwide, thanks to their toxin genes and high virulence. In this context, to develop an alternative to chemical pesticides used to control agricultural and forest pest insects, Bt has been isolated from soils and many insects, and products in the form of formulations have been prepared. It is also known that Bt-based biopesticides are generally more specific and harmless to other organisms. The results demonstrate that countries have different conditions for releasing these organic products to the market and it is often difficult for scientists to obtain a license. This study discusses the current status of bacterial entomopathogens study in Iran and Turkey.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139369828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-15DOI: 10.58465/natprobiotech.2023.03
Omer Gok, Fatma Secer Celik, Canan Eroglu Gunes, E. Kurar
The most common cancer seen in men worldwide is prostate cancer. It ranks second in the deadliest cancer types. Viburnum opulus is a type of tree that produces small red fruits. Shell, fruit, flower and juice of V. opulus are widely used in various fields and traditional medicine. Studies have shown that V. opulus also has an anti-cancer effect. For this purpose, the apoptotic effect of V. opulus was investigated in the LNCaP prostate cancer cell line. LNCaP human prostate cancer cells were treated with V. opulus extract in a 1-200 µg/mL concentration range. IC50 dose of V. opulus extract was determined by cell viability test XTT. Expressions of genes related to apoptosis after extract application were determined by quantitative RT-PCR analysis. V. opulus showed the cytotoxic effect on the LNCaP cell line, and differences in the expressions of apoptosis-related genes were observed. Significant increases in BAX, CASP7, CASP8 and P53 expressions showed that the pro-apoptosis pathway was active, while CASP3 expression was significantly decreased. The extrinsic apoptosis pathway of might be effective due to increased CASP7 and CASP8 gene expression. However, the molecular mechanism of apoptotic activity of V. opulus needs to be investigated in more detail.
{"title":"Investigation of Viburnum opulus L. Apoptotic Effect on LNCaP Prostate Cancer Cell Line","authors":"Omer Gok, Fatma Secer Celik, Canan Eroglu Gunes, E. Kurar","doi":"10.58465/natprobiotech.2023.03","DOIUrl":"https://doi.org/10.58465/natprobiotech.2023.03","url":null,"abstract":"The most common cancer seen in men worldwide is prostate cancer. It ranks second in the deadliest cancer types. Viburnum opulus is a type of tree that produces small red fruits. Shell, fruit, flower and juice of V. opulus are widely used in various fields and traditional medicine. Studies have shown that V. opulus also has an anti-cancer effect. For this purpose, the apoptotic effect of V. opulus was investigated in the LNCaP prostate cancer cell line. LNCaP human prostate cancer cells were treated with V. opulus extract in a 1-200 µg/mL concentration range. IC50 dose of V. opulus extract was determined by cell viability test XTT. Expressions of genes related to apoptosis after extract application were determined by quantitative RT-PCR analysis. V. opulus showed the cytotoxic effect on the LNCaP cell line, and differences in the expressions of apoptosis-related genes were observed. Significant increases in BAX, CASP7, CASP8 and P53 expressions showed that the pro-apoptosis pathway was active, while CASP3 expression was significantly decreased. The extrinsic apoptosis pathway of might be effective due to increased CASP7 and CASP8 gene expression. However, the molecular mechanism of apoptotic activity of V. opulus needs to be investigated in more detail.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"114 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139369711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-15DOI: 10.58465/natprobiotech.2023.01
Fatma Secer Celik, Canan Eroglu Gunes, E. Kurar
Sinapic acid (SA) has been shown to have anti-bacterial, anti-cancer, anti-inflammatory, cardioprotective, hepatoprotective, and neuroprotective effects in pre-clinical and in vitro investigations. Especially in cancer studies, it has been shown that SA has an anti-cancer effect. SA reduced proliferation and induced apoptosis in cancer cells. In this study, the effect of SA on drug resistance in hepatocellular carcinoma was investigated. For this purpose, cells resistant to sorafenib were obtained. According to the gene expression analysis performed in resistant cells, the expression of MDR1, hCE-1 and hCE-2 increased significantly. Then, specific doses of sinapic acid were applied to both Hep3B and resistant Hep3B cell lines. The IC50 dose for Hep3B cells was determined by XTT analysis. The cytotoxic effect of SA was different in resistant and non-resistant cells. The IC50 dose of SA was increased in resistant cells compared to Hep3B cells. The chemotherapeutic applications of herbal chemicals have been widely studied. However, drug resistance against frequently used chemotherapeutics is the biggest obstacle to treatment. This study showed that the dose of SA applied to resistant cells should be increased. This suggests that when the resistance mechanism occurs, the herbal chemicals are also effused out of the cell like drugs. It is necessary to study the intracellular interactions of SA with further studies.
在临床前和体外研究中，西那皮酸（SA）已被证明具有抗菌、抗癌、消炎、保护心脏、保护肝脏和保护神经的作用。特别是在癌症研究中，研究表明南澳大利亚具有抗癌作用。南澳大利亚能减少癌细胞的增殖并诱导其凋亡。本研究调查了 SA 对肝细胞癌耐药性的影响。为此，研究人员获得了对索拉非尼耐药的细胞。根据耐药细胞的基因表达分析，MDR1、hCE-1 和 hCE-2 的表达明显增加。然后，将特定剂量的西那非酸应用于 Hep3B 和耐药 Hep3B 细胞系。通过 XTT 分析确定了 Hep3B 细胞的 IC50 剂量。在耐药和非耐药细胞中，SA 的细胞毒性作用是不同的。与 Hep3B 细胞相比，SA 在耐药细胞中的 IC50 剂量有所增加。中草药化学物质的化疗应用已被广泛研究。然而，对常用化疗药物的耐药性是治疗的最大障碍。这项研究表明，对耐药细胞使用的 SA 剂量应该增加。这表明，当出现耐药机制时，中草药化学物质也会像药物一样从细胞中流出。有必要进一步研究 SA 在细胞内的相互作用。
{"title":"Comparison of the Therapeutic Effect of Sinapic Acid in Drug-Resistant and Non-Resistant Hepatocellular Carcinoma Cells","authors":"Fatma Secer Celik, Canan Eroglu Gunes, E. Kurar","doi":"10.58465/natprobiotech.2023.01","DOIUrl":"https://doi.org/10.58465/natprobiotech.2023.01","url":null,"abstract":"Sinapic acid (SA) has been shown to have anti-bacterial, anti-cancer, anti-inflammatory, cardioprotective, hepatoprotective, and neuroprotective effects in pre-clinical and in vitro investigations. Especially in cancer studies, it has been shown that SA has an anti-cancer effect. SA reduced proliferation and induced apoptosis in cancer cells. In this study, the effect of SA on drug resistance in hepatocellular carcinoma was investigated. For this purpose, cells resistant to sorafenib were obtained. According to the gene expression analysis performed in resistant cells, the expression of MDR1, hCE-1 and hCE-2 increased significantly. Then, specific doses of sinapic acid were applied to both Hep3B and resistant Hep3B cell lines. The IC50 dose for Hep3B cells was determined by XTT analysis. The cytotoxic effect of SA was different in resistant and non-resistant cells. The IC50 dose of SA was increased in resistant cells compared to Hep3B cells. The chemotherapeutic applications of herbal chemicals have been widely studied. However, drug resistance against frequently used chemotherapeutics is the biggest obstacle to treatment. This study showed that the dose of SA applied to resistant cells should be increased. This suggests that when the resistance mechanism occurs, the herbal chemicals are also effused out of the cell like drugs. It is necessary to study the intracellular interactions of SA with further studies.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139369767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.58465/natprobiotech.2022.12
Birsen Atli, Beria Ozcakir, B. Isik, V. Mursaliyeva, R. Mammadov
Fungi are a biological family that includes multicellular and unicellular eukaryotic organisms in general. They are quite numerous and diverse. It is the second largest group of organisms on Earth after insects. Higher fungi (terrestrial fungi) currently constitute a large and efficiently untapped resource of new pharmacological crops with nutritional values are potentially powerful. Antioxidants prevent cellular damage caused by free radicals in living beings. It has been found that 36% of endophytic mushroom extracts with a high phenolic content show strong antioxidant effects. In one study, they reported that the antioxidant activities of the strains Aspergillus sp., A. niger, A. peyronelii and Chaetomium sp. ranged from 50% to 80% and that this antioxidant activity was due to their high phenolic content. In addition to the antioxidant properties of fungi, it has been shown as a result of research that they show antibacterial activity. Macrofungal β-glucans (β-1,6 glycosidic bonds and mainly β-1,3), which are known to have immunomodulatory and antitumor effects in cancer prevention, can be used in treatment strategies. At the same time, it has been shown in studies that these metabolites obtained from fungi cause fewer side effects when used in treatments, unlike chemical drugs. As a result of phytochemical research, the presence of flavonoids, anthraquinones, steroids, terpenoids, coumarins, phenols and tannins in fungal extracts in general was detected, while alkaloids and saponins were not found. It has been determined that the studies on mushrooms have gained versatile uses since they reveal secondary metabolite components with different biological activities in the structure of these group members.
{"title":"Secondary Metabolites in Fungi","authors":"Birsen Atli, Beria Ozcakir, B. Isik, V. Mursaliyeva, R. Mammadov","doi":"10.58465/natprobiotech.2022.12","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.12","url":null,"abstract":"Fungi are a biological family that includes multicellular and unicellular eukaryotic organisms in general. They are quite numerous and diverse. It is the second largest group of organisms on Earth after insects. Higher fungi (terrestrial fungi) currently constitute a large and efficiently untapped resource of new pharmacological crops with nutritional values are potentially powerful. Antioxidants prevent cellular damage caused by free radicals in living beings. It has been found that 36% of endophytic mushroom extracts with a high phenolic content show strong antioxidant effects. In one study, they reported that the antioxidant activities of the strains Aspergillus sp., A. niger, A. peyronelii and Chaetomium sp. ranged from 50% to 80% and that this antioxidant activity was due to their high phenolic content. In addition to the antioxidant properties of fungi, it has been shown as a result of research that they show antibacterial activity. Macrofungal β-glucans (β-1,6 glycosidic bonds and mainly β-1,3), which are known to have immunomodulatory and antitumor effects in cancer prevention, can be used in treatment strategies. At the same time, it has been shown in studies that these metabolites obtained from fungi cause fewer side effects when used in treatments, unlike chemical drugs. As a result of phytochemical research, the presence of flavonoids, anthraquinones, steroids, terpenoids, coumarins, phenols and tannins in fungal extracts in general was detected, while alkaloids and saponins were not found. It has been determined that the studies on mushrooms have gained versatile uses since they reveal secondary metabolite components with different biological activities in the structure of these group members.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117234143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.58465/natprobiotech.2022.11
M. Yildirim, Elif Aksakal, F. B. Ozgeris, Bunyamin Ozgeris, Arzu Gormez
Azides are significant compounds because of their biological effects on bacteria, viruses, fungi, and cancer. The antibacterial and anticancer properties of azide derivatives have been demonstrated in several microbial strains and cancer cell lines. The most significant microorganisms in command of hospital-acquired nosocomial infections include Acinetobacter baumannii (A. baumannii), Methicillin-resistant Staphylococcus aureus (MRSA), Candida albicans (C. albicans), and Candida dubliniensis (C. dubliniensis). These infections have become resistant to a variety of antibiotics. These resistant infections are frequently seen in cancer patients. Novel drug-active compounds are thus required that have both antibacterial and anticancer activity. This study examined previously synthesized 1-azido-5,6,7-trimethoxy-2,3-dihydro-1H-indene (4) for its antibacterial and anticancer activities. Antimicrobial activity was assessed using the disc diffusion technique, and minimum inhibitory concentration values were computed for zone formation in the examined pathogens. The study's findings indicate that while azide derivatives did not exhibit any effect against other pathogens, they suppressed bacterial growth with a zone diameter of 10 mm against A. baumannii. The MIC values of azide derivative against A. baumannii were 3.90 µg/ml. WST-8 analysis in Caco-2 cancer and healthy fibroblast cell line was used to determine the anticancer study. As a result of this analysis, the IC50 value was calculated to be 2.99 µM. There is little anticancer activity. A very low toxic effect of the azide compound on fibroblast cells was also observed. These findings suggest that this azide derivative may be tested as a potential antibacterial and anticancer agent.
{"title":"Biological Evaluation of Azide Derivative as Antibacterial and Anticancer Agents","authors":"M. Yildirim, Elif Aksakal, F. B. Ozgeris, Bunyamin Ozgeris, Arzu Gormez","doi":"10.58465/natprobiotech.2022.11","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.11","url":null,"abstract":"Azides are significant compounds because of their biological effects on bacteria, viruses, fungi, and cancer. The antibacterial and anticancer properties of azide derivatives have been demonstrated in several microbial strains and cancer cell lines. The most significant microorganisms in command of hospital-acquired nosocomial infections include Acinetobacter baumannii (A. baumannii), Methicillin-resistant Staphylococcus aureus (MRSA), Candida albicans (C. albicans), and Candida dubliniensis (C. dubliniensis). These infections have become resistant to a variety of antibiotics. These resistant infections are frequently seen in cancer patients. Novel drug-active compounds are thus required that have both antibacterial and anticancer activity. This study examined previously synthesized 1-azido-5,6,7-trimethoxy-2,3-dihydro-1H-indene (4) for its antibacterial and anticancer activities. Antimicrobial activity was assessed using the disc diffusion technique, and minimum inhibitory concentration values were computed for zone formation in the examined pathogens. The study's findings indicate that while azide derivatives did not exhibit any effect against other pathogens, they suppressed bacterial growth with a zone diameter of 10 mm against A. baumannii. The MIC values of azide derivative against A. baumannii were 3.90 µg/ml. WST-8 analysis in Caco-2 cancer and healthy fibroblast cell line was used to determine the anticancer study. As a result of this analysis, the IC50 value was calculated to be 2.99 µM. There is little anticancer activity. A very low toxic effect of the azide compound on fibroblast cells was also observed. These findings suggest that this azide derivative may be tested as a potential antibacterial and anticancer agent.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116542627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.58465/natprobiotech.2022.8
Damla Ruzgar, Yusuf Akbaba, Bunyamin Ozgeris, Arzu Gormez
The aminoindane ring is the most studied skeletal structure in synthetic chemistry, in the synthesis of intermediates and because it is a biologically active molecule. Bioactive properties of the aminoindane ring include antibacterial, antiviral, antiapoptotic, antipyretic, analgesic, anticonvulsant, and antiparkinsonian activities. The most important bacteria in responsibility of nosocomial infections obtained in hospitals across the world are Acinetobacter baumannii and Methicillin-resistant Staphylococcus aureus. MRSA and A. baumannii have become resistant to numerous antibiotics. The development of novel antibiotic drugs is thus urgently needed. This study as investigated at the antibacterial effects of previously synthsized compounds 8 and 9 on A. baumannii and MRSA disease microorganisms. Studies on antibacterial activity employed disc diffusion and microdilution techniques. The investigation revealed that whereas the pathogenic organisms of compounds 8 and 9 formed zones that inhibited bacterial development, no zones formed in compounds 5, 6, and 7. Compounds 8 and 9's zone diameters against A. baumannii and MRSA were estimated to be between 0.7 and 1.0 mm. The range of MIC values was 3.9025–15.625 µg/ml. These results was envisaged the conclusion that derivatives with an aminoindane ring may function as MRSA and A. baumannii antibacterial agents.
{"title":"Biological Evaluation of Aminoindane Derivatives as Antibacterial Agents","authors":"Damla Ruzgar, Yusuf Akbaba, Bunyamin Ozgeris, Arzu Gormez","doi":"10.58465/natprobiotech.2022.8","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.8","url":null,"abstract":"The aminoindane ring is the most studied skeletal structure in synthetic chemistry, in the synthesis of intermediates and because it is a biologically active molecule. Bioactive properties of the aminoindane ring include antibacterial, antiviral, antiapoptotic, antipyretic, analgesic, anticonvulsant, and antiparkinsonian activities. The most important bacteria in responsibility of nosocomial infections obtained in hospitals across the world are Acinetobacter baumannii and Methicillin-resistant Staphylococcus aureus. MRSA and A. baumannii have become resistant to numerous antibiotics. The development of novel antibiotic drugs is thus urgently needed. This study as investigated at the antibacterial effects of previously synthsized compounds 8 and 9 on A. baumannii and MRSA disease microorganisms. Studies on antibacterial activity employed disc diffusion and microdilution techniques. The investigation revealed that whereas the pathogenic organisms of compounds 8 and 9 formed zones that inhibited bacterial development, no zones formed in compounds 5, 6, and 7. Compounds 8 and 9's zone diameters against A. baumannii and MRSA were estimated to be between 0.7 and 1.0 mm. The range of MIC values was 3.9025–15.625 µg/ml. These results was envisaged the conclusion that derivatives with an aminoindane ring may function as MRSA and A. baumannii antibacterial agents.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131027643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.58465/natprobiotech.2022.9
Siddika Boztas, Buket Er Urganci, Mücahit Seçme, N. Keskin
Nepeta sp. are used diuretic, anti-spasmodic, antiasthmatic, expectorant, cough suppressant, sedative, antiseptic, antipyretic, antiallergy and as a muscle relaxant. Besides, it also has many biological activities such as antioxidant, antibacterial, antiinflammatory, anticancer, antiparasitic, antiplatelet, antiangiogenesis, antimicrobial, antiviral, antifungal, insecticide, cytotoxic, phytotoxic, genotoxic, and apoptotic activities. Osteosarcoma is the most common primary bone cancer in children and adolescents, and its diagnosis is difficult due to the known heterogeneous nature of its pathogenesis. Chemotherapy for osteosarcoma is far from satisfactory, especially because of the presence of metastases or poor response to combination chemotherapy. Various studies have been conducted to evaluate the anti-apoptotic, anticancer, and cytotoxic potentials of different essential oil extracts from different species of the Nepeta. This study was carried out to confirm the cytotoxic and apoptotic effect of essential oil obtained by hydrodistillation from the aerial parts (flower, leaf, seed and stem) of Nepeta italica subsp cadmea. To figure out apoptotic properties of N. italica subsp cadmea. MTT assay, qRT-PCR with Bcl‐2, caspase-9, Caspase-3, caspase-8, caspase-7, caspase-10, Bax gene primer and TUNEL assay accomplished. IC50 dose of N. italica subsp. cadmea oil was 10 μg/ml at 24 hours. Expression analysis showed that caspase8, caspase 10 were upregulated while Bcl-2 was downregulated. TUNEL assay results show the similar number of apoptotic cells in the control group then the Nepeta group. Therefore, it showed pro-apoptotic activity in conjunction with the expression profile.
用于利尿，抗痉挛，平喘，祛痰，止咳，镇静，防腐，解热，抗过敏和作为肌肉松弛剂。此外，它还具有抗氧化、抗菌、抗炎、抗癌、抗寄生虫、抗血小板、抗血管生成、抗菌、抗病毒、抗真菌、杀虫、细胞毒性、植物毒性、基因毒性和细胞凋亡活性等多种生物活性。骨肉瘤是儿童和青少年中最常见的原发性骨癌，由于其发病机制的异质性，其诊断很困难。骨肉瘤的化疗远不能令人满意，特别是因为存在转移或对联合化疗的反应较差。不同种类的荆芥精油提取物的抗凋亡、抗癌和细胞毒作用已被广泛研究。本研究旨在证实从意大利荆芥(Nepeta italica subsp cadmea)地上部分(花、叶、种子和茎)中提取的精油的细胞毒和细胞凋亡作用。目的:探讨意大利青霉(N. italica subsp cadmea)的凋亡特性。完成MTT检测、Bcl‐2、caspase-9、Caspase-3、caspase-8、caspase-7、caspase-10、Bax基因引物的qRT-PCR检测和TUNEL检测。意大利乳霉亚种的IC50剂量。24h时Cadmea油浓度为10 μg/ml。表达分析显示caspase8、caspase 10表达上调，Bcl-2表达下调。TUNEL实验结果显示，对照组的凋亡细胞数与Nepeta组相近。因此，它与表达谱一起显示促凋亡活性。
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Pub Date : 2022-12-15DOI: 10.58465/natprobiotech.2022.10
Büşra Yazıcılar, Y. Chang
Alfalfa is a forage crop that accounts for one of the best sources of protein and is commonly grown all over the world. In vitro callus and embryogenic induction of alfalfa have been investigated previously; however, most of the investigations were almost limited with callus formation. In this study, leaves of 5 Medicago sativa L. cultivars (Alsancak, Sazova, Plato Iside, and Bilensoy) have been used. Influences of culture medium contents and hormones applications on callus and embryogenic callus induction were determined to optimize in vitro culture mediums of alfalfa (0.0125 g kinetin 2,4 D 1 mg mL-1; 1 g kinetin 2,4 D 1 mg mL-1 ; 0.25 g kinetin 2,4 D 2 mg mL-1; 0.5 g kinetin 2,4 D 2 mg mL-1). Callus formation was detected at a rate of 74% in 5 different cultivars used in the experiment. The five alfalfa cultivars were classified into four categories in terms of embryogenic differentiation capacity. The tested alfalfa cultivars varied in their callus formation and embryogenic callus differentiation. Sazova, Alsancak, and Bilensoy were detected for better callus formation; similarly, the same cultivars responded with better embryogenic callus formation in the culture mediums including various hormone concentrations. The present study shows that our methods have beneficial impacts on the somatic embryo induced by alfalfa. However, it depends strongly on genotype, hormone concentrations and the other medium components.
紫花苜蓿是一种饲料作物，是蛋白质的最佳来源之一，在世界各地都有种植。紫花苜蓿的离体愈伤组织和胚胎诱导已有研究;然而，大多数调查几乎局限于愈伤组织的形成。本研究以苜蓿(Medicago sativa L.) 5个品种(Alsancak、Sazova、Plato Iside和Bilensoy)的叶片为材料。研究了培养基含量和激素用量对紫花苜蓿愈伤组织和胚性愈伤组织诱导的影响，优化了紫花苜蓿离体培养基(0.0125 g kinetin 2,4 D 1 mg mL-1;1 g激动素2,4 D 1 mg mL-1;0.25 g激动素2,4 D 2mg mL-1;0.5 g激动素2,4 D 2mg mL-1)。5个不同品种的愈伤组织形成率为74%。根据胚性分化能力将5个苜蓿品种分为4类。不同品种的愈伤组织形成和胚性愈伤组织分化存在差异。Sazova、Alsancak和Bilensoy愈伤组织形成较好;同样，在不同激素浓度的培养基中，相同品种的愈伤组织形成更好。本研究表明，我们的方法对紫花苜蓿诱导的体胚有有益的影响。然而，它在很大程度上取决于基因型、激素浓度和其他培养基成分。
{"title":"Embryogenic Callus Differentiation in Short-Term Callus Derived from Leaf Explants of Alfalfa Cultivars","authors":"Büşra Yazıcılar, Y. Chang","doi":"10.58465/natprobiotech.2022.10","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.10","url":null,"abstract":"Alfalfa is a forage crop that accounts for one of the best sources of protein and is commonly grown all over the world. In vitro callus and embryogenic induction of alfalfa have been investigated previously; however, most of the investigations were almost limited with callus formation. In this study, leaves of 5 Medicago sativa L. cultivars (Alsancak, Sazova, Plato Iside, and Bilensoy) have been used. Influences of culture medium contents and hormones applications on callus and embryogenic callus induction were determined to optimize in vitro culture mediums of alfalfa (0.0125 g kinetin 2,4 D 1 mg mL-1; 1 g kinetin 2,4 D 1 mg mL-1 ; 0.25 g kinetin 2,4 D 2 mg mL-1; 0.5 g kinetin 2,4 D 2 mg mL-1). Callus formation was detected at a rate of 74% in 5 different cultivars used in the experiment. The five alfalfa cultivars were classified into four categories in terms of embryogenic differentiation capacity. The tested alfalfa cultivars varied in their callus formation and embryogenic callus differentiation. Sazova, Alsancak, and Bilensoy were detected for better callus formation; similarly, the same cultivars responded with better embryogenic callus formation in the culture mediums including various hormone concentrations. The present study shows that our methods have beneficial impacts on the somatic embryo induced by alfalfa. However, it depends strongly on genotype, hormone concentrations and the other medium components.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127877057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-29DOI: 10.58465/natprobiotech.2022.7
O. Kocak
Macitentan is an endothelin receptor antagonist drug used in the treatment of pulmonary arterial hypertension (PAH). PAH is defined as a serious disease of the arteries (pulmonary arteries) that connect the lungs to the heart. The aim of our study is to develop and validate a new, sensitive, simple, accurate and reliable UV spectrophotometric method for the quantification of macitentan in human plasma. Using a wavelength of 269 nm, macitentan was quantitatively analyzed. For standard solution concentrations of 1–30 μg/mL and for human plasma concentrations of 2–30 μg/mL, respectively, the method was linear. By computing the correlation coefficient, linearity was determined. In standard solution and human plasma, these values were found to be 0.9996 and 0.9991, respectively. In terms of accuracy, sensitivity, precision, and repeatability, the developed spectrophotometric method was deemed satisfactory. In addition to this, this method could be directly and easily applied to both bulk and human plasma.
{"title":"A New Spectrophotometric Method for The Determination of Macitentan in Human Plasma","authors":"O. Kocak","doi":"10.58465/natprobiotech.2022.7","DOIUrl":"https://doi.org/10.58465/natprobiotech.2022.7","url":null,"abstract":"Macitentan is an endothelin receptor antagonist drug used in the treatment of pulmonary arterial hypertension (PAH). PAH is defined as a serious disease of the arteries (pulmonary arteries) that connect the lungs to the heart. The aim of our study is to develop and validate a new, sensitive, simple, accurate and reliable UV spectrophotometric method for the quantification of macitentan in human plasma. Using a wavelength of 269 nm, macitentan was quantitatively analyzed. For standard solution concentrations of 1–30 μg/mL and for human plasma concentrations of 2–30 μg/mL, respectively, the method was linear. By computing the correlation coefficient, linearity was determined. In standard solution and human plasma, these values were found to be 0.9996 and 0.9991, respectively. In terms of accuracy, sensitivity, precision, and repeatability, the developed spectrophotometric method was deemed satisfactory. In addition to this, this method could be directly and easily applied to both bulk and human plasma.","PeriodicalId":346817,"journal":{"name":"Natural Products and Biotechnology","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115171426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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