Nigerian Journal of Physiological Sciences最新文献

COVID-19 caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) enters the host cells through attachment to the Angiotensin Converting Enzyme-2 receptors (ACE-2) on the host cells. ACE-2 is known to affect renal functions, vasoconstriction and fluid homeostasis. Thus, the impact of SARS-CoV-2 infection on renal functional parameters is worth investigating. Plasma obtained from whole blood samples collected from newly diagnosed COVID-19 patients were analysed for albumin, urea, creatinine, Na, K, Cl and HCO3 using auto analysers. All newly diagnosed patients were immediately admitted for managed at the Infectious Disease Center, Olodo in Ibadan the capital of Oyo State, South Western Nigeria. The results obtained were evaluated to determine the frequency of derangements in the renal parameters of patients with the COVID-19 disease. It was observed that 57.1%, 37.8%, 32.7%, 28.1%, 18.7%, 17.8% and 3.4% of newly diagnosed COVID-19 patients had values of Cl, creatinine, albumin, Na, K, HCO3 and urea respectively outside the reference ranges. While 43.3%, 4.7%, 2.5%, 2.5%, 2.0%, 1.7% and 1.0% of COVID-19 patients had values of Cl, creatinine, Na, K, albumin, Urea and HCO3 respectively above the reference ranges. Of all admitted patients, 33.1%, 30.7%, 25.6%, 16.8%, 16.3%, 13.8% and 1.7% had creatinine, albumin, Na, HCO3, K, Cl and urea values respectively below reference ranges. The changes in renal function parameters of newly diagnosed COVID-19 patients portend that renal failure is imminent in poorly managed COVID-19 patients and this has immunopathology implications during SAR-COV-2 infection.

Mercury is an environmental neurotoxicant that triggers structural and physiological alterations in different brain parts. The hippocampus is associated with learning and memory, and injury to this brain part may lead to behavioural and cognitive changes. Phoenix dactylifera (date palm) has been demonstrated to possess a variety of medical benefits. This study comparatively assessed the neuroprotective property of aqueous and ethanol fruit pulp extracts of P. dactylifera in a rat model of mercury-triggered hippocampal changes using microscopic examinations. Twenty-eight Wistar rats were divided into seven groups (I-VII, n=4). Group I (control) was administered distilled water (2ml/kg); group II was administered mercuric chloride, HgCl2 (5mg/kg); group III was administered vitamin C (100mg/kg) as reference drug +HgCl2; groups IV and V were administered aqueous extract (250mg/kg and 500mg/kg, respectively) +HgCl2, while groups VI and VII were administered ethanol extract (250mg/kg and 500mg/kg, respectively) +HgCl2. Extracts' neuroprotective property were evaluated using histological and histometric assessments of CA1 and CA3 hippocampal sub-regions. Results revealed cytoarchitectural changes including karyopyknosis, basophilic necrosis and remarkably decreased histometric features of hippocampal pyramidal neurons in HgCl2-treated group relative to control. Administration of the extracts remarkably ameliorated mercury-induced degenerative changes by preservation of cytoarchitectural features comparable to reference drug. Comparatively, neuroprotective efficacies of the extracts are relatively similar, especially at doses of 500mg/kg and could be attributed to antioxidant activities of constituent phytochemicals. Results suggest that aqueous and ethanol fruit pulp extracts of P. dactylifera may prove efficacious in ameliorating mercury-triggered microscopic alterations in the hippocampus of Wistar rats.

This study aimed at assessing the knowledge base and perception of telemedicine among veterinary students in Ghana.  It involved a cross sectional survey and online structured questionnaires were administered to 114 veterinary students to access their knowledge of telemedicine and the perceived utilisation, benefits, complexity and disadvantages of telemedicine. Descriptive statistics analysis was performed on the data collected using SPSS version 20 and Pearson Chi-square test was used to determine the significant association between categorical variables which were grouped. Statistical significance was tested at 5% significance level. The students exhibited good knowledge and perception of telemedicine with a mean response of 4.947 ± 1.374 and 3.473±1.115; represented by 86.8% and 78.9% of the students respectively. 92.1% had heard of the term 'telemedicine' from the internet and social media platforms. The level of study of veterinary students had a significant effect (p<0.05) on their knowledge of telemedicine; knowledge increases with an increase in the year of study. Veterinary students showed good knowledge level and perception of telemedicine. However, exposure to the use of telemedicine system is low. Issues of patients' information privacy being threatened by the usage of telemedicine were indicated. Development, incorporation of telemedicine in the curriculum in formative years of veterinary students training is critical to ensure effective and efficient training of students in telemedicine in the COVID era.

  Kolaviron is a mixture of bi-flavonoids from seed Garcinia kola seed, and has been previously shown to exhibit Nrf2 antioxidant-mediated inhibition of neuroinflammation in LPS-activated BV2 microglia. In this study, we investigated neuroprotective effects of kolaviron in LPS-induced memory impairment in rats. Wistar rats (225-250) g was used for this study. Memory impairment was induced with the systematic administration of 250 µg/mg lipopolysaccharide (LPS). The effect of kolaviron on the cognition and learning processes were assessed using the behavioral responses in the Morris water maze model. Effects of LPS injections on the physiological activities were assessed by biochemical assays before and after treatment. Peripheral administration of LPS showed reduction in the cognitive and locomotor process. It also led to reductions in the core body temperature, superoxide dismutase (SOD), and catalase levels, with an increase in Membrane lipid-peroxidation (MDA), intracellular glutathione (GSH) and nitric oxide (NO2). These pro-inflammatory mediators produced in response to LPS are hypothesized to affect cognition, and kolaviron was able to ameliorate the effect by significantly improving the cognitive and learning processes, revealed in the reduction of escape latency and path-length during the probe trial and increase in time spent within the quadrant during retrieval using Morris water maze. Similarly, LPS at 250 µg/kg induced a hypothermic effect in the treated animals. Kolaviron significantly was able to ameliorate the level of SOD and CAT by causing a significant increase while it caused a significant reduction in the level of NO2, GSH, and MDA. Kolaviron has considerable anti-inflammatory potentials, reducing lipopolysaccharide activation of macrophages. The memory-enhancing activity of kolaviron was comparable to Sulindac sulfide (a non-steroidal anti-inflammatory drug).

In this study, the effects of maternal exposure to Bonny Light Crude Oil (BLCO) on reproductive functions of the offspring was investigated in Wistar rats. Ten pregnant rats were divided into two groups (n=5). Group 1 served as the control, it was administered 0.75ml/Kg bwt/day normal saline and Group 2 was administered 0.75ml/Kg bwt/day BLCO. Serum hormonal profile, sperm indices, estrous cycle length and pubertal timing were assessed as measures of reproductive function. Tissue Malondialdehyde, Catalase and SOD activities were assessed as indices of oxidative stress. Results obtained showed that BLCO significantly (p<0.05) reduced birth weight, anogenital distance (AGD) at birth, sperm count, motility and normal morphology, serum testosterone, testicular and epididymal SOD and catalase activities in the male offsprings. However, days of preputial separation, relative weight of testis and epididymis, testicular and epididymal MDA were significantly (p<0.05) raised by gestational exposure to BLCO. In the female offspring, birth weight, AGD at birth, relative weight of ovaries and uterus, SOD, catalase activities, serum LH were significantly reduced by BLCO exposure during gestation. Moreover, uterine and testicular MDA, serum estradiol and FSH were significantly increased by BLCO treatment during gestation. In conclusion, maternal exposure to BLCO during gestation may alter reproductive indices in the offspring and increased occurrence of oxidative stress in reproductive structures in male and female offspring of Wistar rats.

Pregnancy is thus characterized by an anti-inflammatory milieu in the second trimester despite a pro-inflammatory response in the first and third trimesters.  Nonetheless a disproportionate inflammatory response is risky in pregnancy. This retrospective study evaluated the mid-gestational expression of inflammatory and anti-inflammatory cytokines in HIV infected pregnant women at their first antenatal visit. Archived serum samples were collected from seventy (n=70) black pregnant women, attending a primary health care centre in KwaZulu-Natal, South Africa. The demographic and clinical profiles were procured from patient medical records and cytokine levels were measured in all samples. A statistical significance (p<0.05) was noted for IP-10 between the HIV positive and HIV negative groups for the inflammatory, anti-inflammatory cytokines and chemokines when stratified by HIV status. Likewise, IL-7 was statistically different when the HIV population was stratified based on ART usuage. Significant correlations were noted between IL-7 and birthweight (r=0.35, p<0.05); IFN-δ and maternal age (r=-0.27, p<0.05); TNF-α and gestational age (r=0.26, p<0.05); VEGF and systolic blood pressure (r=0.40, p<0.05); IL-4 and gestational age (r=-0.30, p<0.05). A positive correlation was noted for inflammatory IL-1b with anti-inflammatory IL-5, IL-5 and FGF basic; inflammatory IL-2 with anti-inflammatory IL-5, IL-10 as well as FGF basic. A negative correlation between the inflammatory IL-12 with anti-inflammatory IL-1ra and Il-4 as well as that between IL-17A with IL-10 was noted. This study reveals midgestational variation in serum inflammatory and anti-inflammatory immunologic profile of pregnant women, irrespective of the use of antiretroviral therapy.  This disparity in the susceptible HIV infected women will affect progression of pregnancy and encourage fetal morbidity and mortality.

Human immunodeficiency virus (HIV) infection remains a health challenge in Nigeria, and women of reproductive age are disproportionately infected. P53 protein, D-dimer, serum ferritin, CD4 cell count, haemoglobin concentration and haematocrit levels were measured among non-pregnant women of reproductive age living with HIV infection in order to assess the impact of HIV infection on maternal health. A hundred and sixty-two subjects categorised into three groups of 54 persons each involving; newly diagnosed, subjects on highly active antiretroviral therapy (HAART) and apparently healthy control subjects were recruited. Blood samples were analyzed for haemoglobin concentration, haematocrit, CD4 cell count, serum ferritin, D-dimer and p53 protein levels by standard methods. The CD4 cell count, serum p53 protein, and Hb Conc. were significantly lower, while serum ferritin was higher in the newly diagnosed group (p=0.001), followed by the group on HAART (p=0.001) compared to the controls. D-dimer level was significantly lower in the control group (2899.11±670.73pg/ml) than both newly diagnosed (4842.44±489.40pg/ml) and HAART (4660.31±519.83pg/ml) groups, while significant decrease in haematocrit was observed between the newly diagnosed group (0.336±0.07l/l) as against both treated (0.378±0.04l/l) and control (0.362±0.02l/l) groups. D-dimer correlated negatively with serum p53 protein level  among the newly diagnosed subjects  and with Hb Conc. among subjects undergoing treatment.  The study concludes that women of reproductive age living with HIV infection showed higher D-dimer and lower tumour suppression protein levels as well as anaemia and reduced immune response. The newly diagnosed subjects were more affected.

Tooth-wear signatures obtained from maxillary carnassial fourth premolar teeth of raccoons in three ecologic regions in Nigeria testified to segregations in diet of the species with more abrasive diet in specimens from coastal south-western areas compared to more vegetal diet content of those from middle belt and northern areas. Endoloph assessments showed sexually dimorphic mesowear signals between and within locations suggestive that males are more exposed to dental wears compared to females; Male and female specimens from rainforest zone had 40.2% and 34.2% respectively, Sudan Savanna zone had 46.8% and 40.6% for females and males while 67.6% and 44.3% for Sahel zone specimens in similar order. We investigated dietary resource use for sustained survivability within limits of interspecific spatial overlaps using seasonal rainfall indices between two years. There was 86% per high dental occlusal surface relief in the specimens from the savannas while 32% per low relief was observed in South-Western badgers teeth samples. This study observed a change in habitat use as a predisposing factor to sub-regional dental wear differences among age groups as well as sexes of species from three geographic climatic areas. The richness of the eco-habitat/life expectancy found in the rain forest can be ascribed to diet availability which is reduced in the savanna areas. The study suggests minimal change in habitat use as a predisposing factor in sub-regional species dental relief differences observed among age groups and sexes of the species from three geographic climatic areas and also represents quality of the eco-habitats.

Many physiological factors such as ethnicity, sex and blood group have been proven to have roles to play in determining the bleeding and clotting time of an individual. Earlier studies had conflicting results about the bleeding and clotting time in an individual concerning physiological factors. The objective of the study was to study the bleeding and clotting time of an individual with respect to their sex and blood group. This cross-sectional study involved 134 undergraduate students between the ages of 17-25years. Blood group was determined using a standard anti-sera, clotting time was determined using the capillary method while the bleeding time was determined using Duke's filter paper method. In this study, blood group B was predominate. The bleeding time and clotting time were significantly longer in females compared to that of males. The clotting time was longer in individuals with blood group O but the bleeding time among the blood groups was not significantly different. However, the subject size needs to be increased in further studies.

Bats and human biological structures are believed to be similar in terms of phylogeny, reproductive biology, and early development. Adequate knowledge of placental morphology will have important implications for research and in comparative anatomy. This report is a part of on-going studies on the African fruit bat species and is a case report from an incidental discovery of the foetus and placenta in an African fruit bat (Epomops franqueti) captured for research.