Pub Date : 2022-09-30DOI: 10.53346/wjcps.2022.1.1.0026
Modibo Coulibaly, Jean Luis Konan, Mary Laure Hauhouot Attoungbre, Dagui Monnet
Background: the blood electrolyte analysis is a routine laboratory test, the proper execution of which would help in the diagnosis of hydro-electrolyte disorders. We undertook to assess the quality of the sodium and potassium from the pre-pre-analytical phase to the post-analytical phase. Material and Methods: This was a cross-sectional study which took in the laboratory of biochemistry at the Institute of Cardiology, Abidjan, Ivory Coast from March 1st to March 31, 2009. We used the flame photometer to measure the sodium and potassium electrolytes level in the internal control Exatrol-Normal from Biolabo® and those of the clinical samples. The pre-pre-analytical quality indicators depending on the physician’s order, the pre-analytical quality indicators and the post-analytical indicators under the control of the laboratory and based on the NF standard ISO 15189 version 2012 have been determined. Data were captured into Microsoft Access [Microsoft Corporation, Redmond, WA] and then imported and analyzed using QI Macros SPC Software for Excel®. The monthly dispersion parameters of the Exatrol Normal were used to establish the Levey-Jennings diagram and the Wesgard’s rules were used for the interpretation. Results: a total of 112 electrolytes analysis order were received. For the pre-pre-analytical phase, the analysis of these requests revealed that 81 (72.3%) requests carried no clinical information. The non-compliance of the samples were mainly represented by the sampling under tight tourniquet 4 (3.6%), followed by the non-respect of the succession tubes during multiple sampling 3 (2.7%). For the analytical phase, the monthly Levey-Jennings diagram showed a dispersion of the Exatrol-Normal® values between the mean plus or minus 2 standard deviations [m ± 2SD]: 139.34 ± 2.84 mmol/L for sodium (Na+). For the potassium (K+), the values of Exatrol-Normal® were between [m± SD]: 4.2±0.78 mmol/L. The interpretation of the two Levey-Jennings diagrams by Wesgard’s rules did not found any statistically significant mistake with regard to the distribution of Na+ and K+ levels. For clinical samples, isolated hyponatremia was the most common disturbance (30.4%) followed by isolated hypokalemia (12.5%). At the post-analytical phase we observed a mean turnaround time of 34 minutes with extremes ranging from 23 to 95 minutes. One case (0.9%) of transcription error was noted. Conclusion: the internal quality control process is applied in the clinical biochemistry laboratory at the Institute of Cardiology, Abidjan. A systematic verification system of the different phases of the analytical process makes it possible to identify errors at all levels of the analytical process and to take corrective action if necessary. Better collaboration between clinicians requesting electrolyte analysis and biologists performing the analysis is necessary to improve the pre-pre-analytical phase and, beyond that, better patient care.
{"title":"Internal quality control of Na+ and K+ at clinical biochemistry laboratory","authors":"Modibo Coulibaly, Jean Luis Konan, Mary Laure Hauhouot Attoungbre, Dagui Monnet","doi":"10.53346/wjcps.2022.1.1.0026","DOIUrl":"https://doi.org/10.53346/wjcps.2022.1.1.0026","url":null,"abstract":"Background: the blood electrolyte analysis is a routine laboratory test, the proper execution of which would help in the diagnosis of hydro-electrolyte disorders. We undertook to assess the quality of the sodium and potassium from the pre-pre-analytical phase to the post-analytical phase. Material and Methods: This was a cross-sectional study which took in the laboratory of biochemistry at the Institute of Cardiology, Abidjan, Ivory Coast from March 1st to March 31, 2009. We used the flame photometer to measure the sodium and potassium electrolytes level in the internal control Exatrol-Normal from Biolabo® and those of the clinical samples. The pre-pre-analytical quality indicators depending on the physician’s order, the pre-analytical quality indicators and the post-analytical indicators under the control of the laboratory and based on the NF standard ISO 15189 version 2012 have been determined. Data were captured into Microsoft Access [Microsoft Corporation, Redmond, WA] and then imported and analyzed using QI Macros SPC Software for Excel®. The monthly dispersion parameters of the Exatrol Normal were used to establish the Levey-Jennings diagram and the Wesgard’s rules were used for the interpretation. Results: a total of 112 electrolytes analysis order were received. For the pre-pre-analytical phase, the analysis of these requests revealed that 81 (72.3%) requests carried no clinical information. The non-compliance of the samples were mainly represented by the sampling under tight tourniquet 4 (3.6%), followed by the non-respect of the succession tubes during multiple sampling 3 (2.7%). For the analytical phase, the monthly Levey-Jennings diagram showed a dispersion of the Exatrol-Normal® values between the mean plus or minus 2 standard deviations [m ± 2SD]: 139.34 ± 2.84 mmol/L for sodium (Na+). For the potassium (K+), the values of Exatrol-Normal® were between [m± SD]: 4.2±0.78 mmol/L. The interpretation of the two Levey-Jennings diagrams by Wesgard’s rules did not found any statistically significant mistake with regard to the distribution of Na+ and K+ levels. For clinical samples, isolated hyponatremia was the most common disturbance (30.4%) followed by isolated hypokalemia (12.5%). At the post-analytical phase we observed a mean turnaround time of 34 minutes with extremes ranging from 23 to 95 minutes. One case (0.9%) of transcription error was noted. Conclusion: the internal quality control process is applied in the clinical biochemistry laboratory at the Institute of Cardiology, Abidjan. A systematic verification system of the different phases of the analytical process makes it possible to identify errors at all levels of the analytical process and to take corrective action if necessary. Better collaboration between clinicians requesting electrolyte analysis and biologists performing the analysis is necessary to improve the pre-pre-analytical phase and, beyond that, better patient care.","PeriodicalId":350635,"journal":{"name":"World Journal of Chemical and Pharmaceutical Sciences","volume":"142 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128782726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-30DOI: 10.53346/wjcps.2022.1.1.0024
Sanchita Dewanjee, Farzia Akter, Md. Shahadat Hossain, Md. Ashraful Islam, Mohammad Hamid Al Muktadir, Md. Tofikul Islam, Md Kayes Mahmud
The present study was conducted to assess the cytotoxicity, thrombolytic, anthelmintic and antioxidant activity of mehanolic extract of Litsea monopetala (Family: Lauraceae) leaves in laboratory using in vitro methods. Cytotoxicity test was done by brine shrimp lethality bioassay where the extract concentration was 400, 200, 100, 50, 25, 12.5, 6.25, 3.125, 1.5625, 0.78125 (µg/ml). In vitro thrombolytic activity of Litsea monopetala was performed by clot lysis method using extract concentration 2.5, 5, 10 and 20 (mg/mL) in saline water. Anthelmintic activity test was done by using adult earthworms where 10, 20, 40, 60, 80 (mg/ml) extract concentration were used. Finally antioxidant activity was determined by total phenolic content determination using Folin-Ciocalteu reagent. The Litsea monopetala extract showed cytotoxic activity against brine shrimp nauplii and LC50 value was 41.05(µg/ml) and the investigated thrombolytic activity in our research was 9.52, 9.49, 13.64 and 17.50 % respectively as % of clot lysis. The paralysis time were at 76.75 min, 60 min, 51.75 min, 44.5 min and 64.5 min and death were at 90.50min, 63.75min, 55.50min, 44.75min and 71min. respectively. The Litsea monopetala extract displayed significant antioxidant activity which was 20.75 (mg of GAE / gm) of extracts. The activities observed could be attributed to presence of some of the phytochemicals which have been related with cytotoxic, thrombolytic, anthelmintic and antioxidant property.
{"title":"In vitro cytotoxic, thrombolytic, anthelmintic and antioxidant activities of Litsea monopetala: A medicinal plant","authors":"Sanchita Dewanjee, Farzia Akter, Md. Shahadat Hossain, Md. Ashraful Islam, Mohammad Hamid Al Muktadir, Md. Tofikul Islam, Md Kayes Mahmud","doi":"10.53346/wjcps.2022.1.1.0024","DOIUrl":"https://doi.org/10.53346/wjcps.2022.1.1.0024","url":null,"abstract":"The present study was conducted to assess the cytotoxicity, thrombolytic, anthelmintic and antioxidant activity of mehanolic extract of Litsea monopetala (Family: Lauraceae) leaves in laboratory using in vitro methods. Cytotoxicity test was done by brine shrimp lethality bioassay where the extract concentration was 400, 200, 100, 50, 25, 12.5, 6.25, 3.125, 1.5625, 0.78125 (µg/ml). In vitro thrombolytic activity of Litsea monopetala was performed by clot lysis method using extract concentration 2.5, 5, 10 and 20 (mg/mL) in saline water. Anthelmintic activity test was done by using adult earthworms where 10, 20, 40, 60, 80 (mg/ml) extract concentration were used. Finally antioxidant activity was determined by total phenolic content determination using Folin-Ciocalteu reagent. The Litsea monopetala extract showed cytotoxic activity against brine shrimp nauplii and LC50 value was 41.05(µg/ml) and the investigated thrombolytic activity in our research was 9.52, 9.49, 13.64 and 17.50 % respectively as % of clot lysis. The paralysis time were at 76.75 min, 60 min, 51.75 min, 44.5 min and 64.5 min and death were at 90.50min, 63.75min, 55.50min, 44.75min and 71min. respectively. The Litsea monopetala extract displayed significant antioxidant activity which was 20.75 (mg of GAE / gm) of extracts. The activities observed could be attributed to presence of some of the phytochemicals which have been related with cytotoxic, thrombolytic, anthelmintic and antioxidant property.","PeriodicalId":350635,"journal":{"name":"World Journal of Chemical and Pharmaceutical Sciences","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126262462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-30DOI: 10.53346/wjcps.2022.1.1.0025
Muhammad Arshad Ullah, Ali Hassan
Melissa officinalis L, also known as lemon balm, bee balm, honey balm, is a perennial herb. Lemon balm occurs naturally in sandy and scrubby areas but also grown on damp wasteland, at elevations ranging from sea level to the mountains. Melissa officinalis is cost-effective, and compared with the economic indicators of traditional crops grown on fertilized land; this herb provides much higher profits. The leaf of Melissa officinalis contains flavonoids (quercitrin, rhamnocitrin, luteolin), polyphenolic compounds (rosmarinic acid, caffeic acid, and protocatechuic acid), monoterpenoid aldehyde, monoterpene glycosides, triterpenes (ursolic and oleanolic acids), sesquiterpenes, tannins, and essential oils (citral). Antimicrobial activities of the extracts and of rosmarinic acid of this plant were evaluated and were confirmed. The essential oil of Melissa officinalis L was shown to have anti-inflammatory activities, in treating various diseases associated with inflammation and pain. Melissa officinalis L relieves stress-related effects. Extract have the potential to prevent oxidative damage by preventing free radical– mediated oxidative stress. Melissa officinalis showed strong reducing power and exhibited a significant inhibition of deoxyribose degradation. The high phenolic content and radical scavenging activities of extracts of Melissa officinalis L was confirmed.
{"title":"Medicinal benefits of lemon balm (Melissa officinalis) for human health","authors":"Muhammad Arshad Ullah, Ali Hassan","doi":"10.53346/wjcps.2022.1.1.0025","DOIUrl":"https://doi.org/10.53346/wjcps.2022.1.1.0025","url":null,"abstract":"Melissa officinalis L, also known as lemon balm, bee balm, honey balm, is a perennial herb. Lemon balm occurs naturally in sandy and scrubby areas but also grown on damp wasteland, at elevations ranging from sea level to the mountains. Melissa officinalis is cost-effective, and compared with the economic indicators of traditional crops grown on fertilized land; this herb provides much higher profits. The leaf of Melissa officinalis contains flavonoids (quercitrin, rhamnocitrin, luteolin), polyphenolic compounds (rosmarinic acid, caffeic acid, and protocatechuic acid), monoterpenoid aldehyde, monoterpene glycosides, triterpenes (ursolic and oleanolic acids), sesquiterpenes, tannins, and essential oils (citral). Antimicrobial activities of the extracts and of rosmarinic acid of this plant were evaluated and were confirmed. The essential oil of Melissa officinalis L was shown to have anti-inflammatory activities, in treating various diseases associated with inflammation and pain. Melissa officinalis L relieves stress-related effects. Extract have the potential to prevent oxidative damage by preventing free radical– mediated oxidative stress. Melissa officinalis showed strong reducing power and exhibited a significant inhibition of deoxyribose degradation. The high phenolic content and radical scavenging activities of extracts of Melissa officinalis L was confirmed.","PeriodicalId":350635,"journal":{"name":"World Journal of Chemical and Pharmaceutical Sciences","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131181915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was conducted to detect possible chemicals (phytoconstituents), and investigate antioxidant, antimicrobial and thrombolytic activities of the extract of Aphanamixis polystachya (stem bark). Phytochemical screening was carried out using the standard test methods of different chemical group. For investigating the antioxidant activity, two complementary test methods namely DPPH free radical scavenging assay and total phenolic content determination were carried out. For the evaluation of in Vitro antimicrobial activity, disc diffusion method, and to determine the thrombolytic activity, the method of Prasad et al., 2007 with minor modifications were used. The bark extracts were a rich source of phytochemicals. In DPPH free radical scavenging test, the Carbon tetra chloride soluble fraction showed the highest free radical scavenging activity with IC50 value 19.86 µg/ml. while compared to that of the reference standards ascorbic acid. Aphanamixis polystachya was also found as a good source of total phenolic contents. Moreover, the extracts revealed broad spectrum antimicrobial activity at the concentration of 400 µg/disc. By comparing with the negative control the mean clot lysis % was significant (p value <0.0009). Therefore, further studies are suggested to determine the active compounds responsible for the biological activities of the plant extracts.
摘要本研究主要对多stachya茎皮提取物进行化学成分检测，并对其抗氧化、抗菌和溶栓活性进行研究。采用不同化学基团的标准试验方法进行植物化学筛选。为了研究其抗氧化活性，采用了DPPH自由基清除试验和总酚含量测定两种互补试验方法。体外抗菌活性评价采用圆盘扩散法，溶栓活性测定采用Prasad et al.， 2007的方法，稍作修改。树皮提取物是植物化学物质的丰富来源。在DPPH自由基清除试验中，四氯化碳可溶性组分的自由基清除活性最高，IC50值为19.86µg/ml。同时与参比标准抗坏血酸进行比较。多水参也被发现是总酚含量的良好来源。此外，在浓度为400µg/盘时，提取物显示出广谱抗菌活性。与阴性对照比较，平均凝块溶解率显著(p值<0.0009)。因此，建议进一步研究确定植物提取物中具有生物活性的活性化合物。
{"title":"A Study on Aphanamixis polystachya for evaluation of phytochemical and pharmacological properties","authors":"Md. Eleas Kobir, Sanchita Dewanjee, Md. Shahadat Hossain, Mohammad Hasem Babu, Nusrat Jahan, Tanoy Saha, Shoukat Akbar, Md Sumon Miah, Md Shafiqul Islam, Md. Abdur Rahman, Amdadul Hoque, Sujoy Das, Md. Monirul Islam","doi":"10.53346/wjcps.2022.1.1.0022","DOIUrl":"https://doi.org/10.53346/wjcps.2022.1.1.0022","url":null,"abstract":"The present study was conducted to detect possible chemicals (phytoconstituents), and investigate antioxidant, antimicrobial and thrombolytic activities of the extract of Aphanamixis polystachya (stem bark). Phytochemical screening was carried out using the standard test methods of different chemical group. For investigating the antioxidant activity, two complementary test methods namely DPPH free radical scavenging assay and total phenolic content determination were carried out. For the evaluation of in Vitro antimicrobial activity, disc diffusion method, and to determine the thrombolytic activity, the method of Prasad et al., 2007 with minor modifications were used. The bark extracts were a rich source of phytochemicals. In DPPH free radical scavenging test, the Carbon tetra chloride soluble fraction showed the highest free radical scavenging activity with IC50 value 19.86 µg/ml. while compared to that of the reference standards ascorbic acid. Aphanamixis polystachya was also found as a good source of total phenolic contents. Moreover, the extracts revealed broad spectrum antimicrobial activity at the concentration of 400 µg/disc. By comparing with the negative control the mean clot lysis % was significant (p value <0.0009). Therefore, further studies are suggested to determine the active compounds responsible for the biological activities of the plant extracts.","PeriodicalId":350635,"journal":{"name":"World Journal of Chemical and Pharmaceutical Sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134508314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-30DOI: 10.53346/wjcps.2022.1.1.0023
Md. Shahadat Hossain, Sanchita Dewanjee, Md. Ashraful Islam, Mohammad Hamid Al Muktadir, Sushmita Karmokar, Md Kayes Mahmud, Mohammad Hasem Babu, Sujoy Das, Mohammad Shariful Islam, Sonia Ferdousy
The present study was conducted to detect possible phytochemicals and evaluate antioxidant, antimicrobial and cytotoxic activities of the extract of Ardisia solanacea. Phytochemical screening was carried out using the standard test methods of different chemical group. For investigating the antioxidant activity, two complementary test methods namely DPPH free radical scavenging assay and total phenolic content determination were carried out. For the evaluation of in vitro antimicrobial activity, disc diffusion method was used. Evaluation of cytotoxic activity was done using the brine shrimp lethality bioassay. In DPPH free radical scavenging test, the petroleum ether soluble fraction showed the highest free radical scavenging activity with IC50 value 40.04 μg/ml. while compared to that of the reference standards ascorbic acid. Ardisia solanacea was also found as a good source of total phenolic contents. Moreover, the extracts revealed moderate antimicrobial activity at the concentration of 400 μg/disc. In cytotoxic activity test, the petroleum ether soluble fraction showed significant cytotoxic potential (LC50 value of 0.703 μg/ml) among all the fractions comparing with that of standard vincristine (0.544 μg/ml). Therefore, further studies are suggested to determine the active compounds responsible for the pharmacological activities of the plant extracts.
{"title":"Characterization of phytochemicals and determination of antioxidant, antimicrobial and cytotoxic properties of the medicinal plant Ardisia solanacea","authors":"Md. Shahadat Hossain, Sanchita Dewanjee, Md. Ashraful Islam, Mohammad Hamid Al Muktadir, Sushmita Karmokar, Md Kayes Mahmud, Mohammad Hasem Babu, Sujoy Das, Mohammad Shariful Islam, Sonia Ferdousy","doi":"10.53346/wjcps.2022.1.1.0023","DOIUrl":"https://doi.org/10.53346/wjcps.2022.1.1.0023","url":null,"abstract":"The present study was conducted to detect possible phytochemicals and evaluate antioxidant, antimicrobial and cytotoxic activities of the extract of Ardisia solanacea. Phytochemical screening was carried out using the standard test methods of different chemical group. For investigating the antioxidant activity, two complementary test methods namely DPPH free radical scavenging assay and total phenolic content determination were carried out. For the evaluation of in vitro antimicrobial activity, disc diffusion method was used. Evaluation of cytotoxic activity was done using the brine shrimp lethality bioassay. In DPPH free radical scavenging test, the petroleum ether soluble fraction showed the highest free radical scavenging activity with IC50 value 40.04 μg/ml. while compared to that of the reference standards ascorbic acid. Ardisia solanacea was also found as a good source of total phenolic contents. Moreover, the extracts revealed moderate antimicrobial activity at the concentration of 400 μg/disc. In cytotoxic activity test, the petroleum ether soluble fraction showed significant cytotoxic potential (LC50 value of 0.703 μg/ml) among all the fractions comparing with that of standard vincristine (0.544 μg/ml). Therefore, further studies are suggested to determine the active compounds responsible for the pharmacological activities of the plant extracts.","PeriodicalId":350635,"journal":{"name":"World Journal of Chemical and Pharmaceutical Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128548195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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