Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.2044
Dahvia Nursriyanti, Ani Kartini, M. Mutmainnah
Hepatocellular carcinoma (HCC) is the most common malignant tumor with a poor prognosis and is known as the silent killer. The currently recommended HCC staging system is Barcelona Clinic Liver Cancer (BCLC). The Alpha-fetoprotein (AFP) test is still used to manage HCC patients. The purpose was to find out the AFP comparations and to decide cut-off values for each stage according to BCLC. Cross-sectional retrospective study using secondary data from medical records of Outpatients Clinic and Inpatients from January 2016 to October 2021 at Dr. Wahidin Sudirohusodo Hospital. A total of 432 samples based on BCLC were taken with AFP values and analyzed in this study. The male sample was 72.7%. The highest age is 46-65 years (62.7%). The etiology of HCC mostly is hepatitis B (72.5%). The most BCLC systems were BCLC B (53.7%), followed by BCLC A (25.9%), BCLC D (12.5%), and BCLC C (7.87%). There was a significant difference in AFP values between BCLC stages and the highest significance was BCLC D, followed by BCLC C, B, and A (p=0.05). The cut-off results values at the BCLC stage were BCLC A <21.84 ng/mL, BCLC B: 21.84 ng/mL (sensitivity 90.3%, specificity 85.7%), BCLC C: 478 ng/mL (sensitivity and specificity 100%), BCLC D: 7.693 ng/mL (sensitivity and specificity 100%). There was a significant difference in AFP values between BCLC stages and the highest is BCLC D. Based on the results of calculating the cut-off value, which has good sensitivity and specificity, AFP can be used as a determinant of stage progression of HCC.
{"title":"Analysis of Alfa-Fetoprotein as A Staging Determiner of Hepatocellular Carcinoma Progresivity","authors":"Dahvia Nursriyanti, Ani Kartini, M. Mutmainnah","doi":"10.24293/ijcpml.v29i3.2044","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.2044","url":null,"abstract":"Hepatocellular carcinoma (HCC) is the most common malignant tumor with a poor prognosis and is known as the silent killer. The currently recommended HCC staging system is Barcelona Clinic Liver Cancer (BCLC). The Alpha-fetoprotein (AFP) test is still used to manage HCC patients. The purpose was to find out the AFP comparations and to decide cut-off values for each stage according to BCLC. Cross-sectional retrospective study using secondary data from medical records of Outpatients Clinic and Inpatients from January 2016 to October 2021 at Dr. Wahidin Sudirohusodo Hospital. A total of 432 samples based on BCLC were taken with AFP values and analyzed in this study. The male sample was 72.7%. The highest age is 46-65 years (62.7%). The etiology of HCC mostly is hepatitis B (72.5%). The most BCLC systems were BCLC B (53.7%), followed by BCLC A (25.9%), BCLC D (12.5%), and BCLC C (7.87%). There was a significant difference in AFP values between BCLC stages and the highest significance was BCLC D, followed by BCLC C, B, and A (p=0.05). The cut-off results values at the BCLC stage were BCLC A <21.84 ng/mL, BCLC B: 21.84 ng/mL (sensitivity 90.3%, specificity 85.7%), BCLC C: 478 ng/mL (sensitivity and specificity 100%), BCLC D: 7.693 ng/mL (sensitivity and specificity 100%). There was a significant difference in AFP values between BCLC stages and the highest is BCLC D. Based on the results of calculating the cut-off value, which has good sensitivity and specificity, AFP can be used as a determinant of stage progression of HCC.\u0000 ","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126575801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.2012
Ellen Kurniawati Tungka, Yuyun Widaningsih, Fitriani Mangarengi
Patients with Acute Kidney Injury (AKI) who require hemodialysis had been reported to have a mortality rate of 50-70% in the last 30 years. Platelet and platelet index are two of the important hematological markers to be analyzed in AKI. This study aimed to analyze the levels of MPV, PCT, PDW, and total platelets in patients with AKI. A retrospective study using medical record data of 122 patients with AKI from January 2019 to December 2020 was conducted in Dr. Wahidin Sudirohusodo Hospital, Makassar. Patients were grouped based on disease outcomes and consisted of patients who died in the hospital and patients who recovered. Measurement of MPV, PCT, PDW, and platelet count was performed using a hematology analyzer. Mann-Whitney and independent T-tests were used for statistical analysis. The subjects of this study consisted of 80 AKI patients who died and 42 who recovered, the most affected age was > 56 years old. The mean MPV was significantly higher in subjects who died in the hospital (10.31±1.53 fL) than in patients who recovered (9.5±1.39 fL) (p<0.01). Mean PCT was higher in subjects who died in the hospital (0.85%) than in patients who recovered (0.18%), despite statistical insignificance (p>0.05). Mean PDW was higher in subjects who died in the hospital (14.2 fL) than in patients who recovered (13.2 fL), despite statistical insignificance (p>0.05). Mean platelet was lower in subjects who died in the hospital (174.3 x109L) than in patients who recovered (215.6 x109L), despite statistical insignificance (p>0.05). Mean platelet volume levels were elevated in AKI patients with poor outcomes, possibly associated with the pathogenesis of more severe inflammation caused by hyperaggregation and peripheral destruction of platelets, which provide positive feedback to the bone marrow to produce larger and more active platelets. The MPV levels were significantly higher in AKI patients with poor outcomes, whereas PCT, PDW, and total platelet levels were not significant.
{"title":"The Analysis of MPV, Plateletcrit, Platelet Distribution Width, and Total Platelets in AKI","authors":"Ellen Kurniawati Tungka, Yuyun Widaningsih, Fitriani Mangarengi","doi":"10.24293/ijcpml.v29i3.2012","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.2012","url":null,"abstract":"Patients with Acute Kidney Injury (AKI) who require hemodialysis had been reported to have a mortality rate of 50-70% in the last 30 years. Platelet and platelet index are two of the important hematological markers to be analyzed in AKI. This study aimed to analyze the levels of MPV, PCT, PDW, and total platelets in patients with AKI. A retrospective study using medical record data of 122 patients with AKI from January 2019 to December 2020 was conducted in Dr. Wahidin Sudirohusodo Hospital, Makassar. Patients were grouped based on disease outcomes and consisted of patients who died in the hospital and patients who recovered. Measurement of MPV, PCT, PDW, and platelet count was performed using a hematology analyzer. Mann-Whitney and independent T-tests were used for statistical analysis. The subjects of this study consisted of 80 AKI patients who died and 42 who recovered, the most affected age was > 56 years old.\u0000The mean MPV was significantly higher in subjects who died in the hospital (10.31±1.53 fL) than in patients who recovered (9.5±1.39 fL) (p<0.01). Mean PCT was higher in subjects who died in the hospital (0.85%) than in patients who recovered (0.18%), despite statistical insignificance (p>0.05). Mean PDW was higher in subjects who died in the hospital (14.2 fL) than in patients who recovered (13.2 fL), despite statistical insignificance (p>0.05). Mean platelet was lower in subjects who died in the hospital (174.3 x109L) than in patients who recovered (215.6 x109L), despite statistical insignificance (p>0.05). Mean platelet volume levels were elevated in AKI patients with poor outcomes, possibly associated with the pathogenesis of more severe inflammation caused by hyperaggregation and peripheral destruction of platelets, which provide positive feedback to the bone marrow to produce larger and more active platelets. The MPV levels were significantly higher in AKI patients with poor outcomes, whereas PCT, PDW, and total platelet levels were not significant.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116760417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-02DOI: 10.24293/ijcpml.v26i1.2181
Nordjannah Nordjannah, Ani Kartini, Darmawaty Er
Pleural effusion is an abnormal accumulation of pleural fluid in the pleural cavity due to excessive transudation or exudation. Light's criteria is used as the standard method to distinguish between exudates and transudates. Some recent studies reported misclassifications which led to development of several alternative criteria, such as Heffner's criteria. The purpose of this study was to determine the sensitivity and specificity of Heffner's criteria to determine the type of pleural effusion. This research was an observational study with a cross-sectional method using a pleural effusion of patients at the Clinical Pathology Laboratory Installation at the Wahidin Sudirohusodo Hospital in July 2018. Total protein, LDH, and cholesterol levels were measured in all samples that met the inclusion and exclusion criteria. There were 45 pleural effusion samples that consisted of 30 transudate and 15 exudate samples. Based on clinical diagnosis, the Light's criteria showed 3 misclassifications and Heffner's criteria obtained showed 2 misclassifications. Based on the data above, the statistical data showed that Light's criteria had a sensitivity of 96.7% and specificity of 86.7%. Heffner's criteria had a sensitivity of 100% and specificity of 86.7%. Heffner's criteria can be used an alternative method to determine the type of pleural effusion because it showed a better sensitivity and specificity than Light's criteria.
{"title":"Evaluation of Pleural Effusion Type Determination Based on Light's and Heffner's Criteria","authors":"Nordjannah Nordjannah, Ani Kartini, Darmawaty Er","doi":"10.24293/ijcpml.v26i1.2181","DOIUrl":"https://doi.org/10.24293/ijcpml.v26i1.2181","url":null,"abstract":"Pleural effusion is an abnormal accumulation of pleural fluid in the pleural cavity due to excessive transudation or exudation. Light's criteria is used as the standard method to distinguish between exudates and transudates. Some recent studies reported misclassifications which led to development of several alternative criteria, such as Heffner's criteria. The purpose of this study was to determine the sensitivity and specificity of Heffner's criteria to determine the type of pleural effusion. This research was an observational study with a cross-sectional method using a pleural effusion of patients at the Clinical Pathology Laboratory Installation at the Wahidin Sudirohusodo Hospital in July 2018. Total protein, LDH, and cholesterol levels were measured in all samples that met the inclusion and exclusion criteria. There were 45 pleural effusion samples that consisted of 30 transudate and 15 exudate samples. Based on clinical diagnosis, the Light's criteria showed 3 misclassifications and Heffner's criteria obtained showed 2 misclassifications. Based on the data above, the statistical data showed that Light's criteria had a sensitivity of 96.7% and specificity of 86.7%. Heffner's criteria had a sensitivity of 100% and specificity of 86.7%. Heffner's criteria can be used an alternative method to determine the type of pleural effusion because it showed a better sensitivity and specificity than Light's criteria.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134215452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.24293/ijcpml.v29i2.1984
Andy Inna Agustina, Samad R, Juliyani S, M. Arif
Blood donation often causes the depletion of iron stores in the body resulting in anemia. One of the markers to assess the hemoglobin content in reticulocytes is Ret-He. This study aims to analyze Ret-He in routine and non-routine blood donors. This was a cross-sectional study using primary data from routine and non-routine blood donors at the Blood Transfusion Technical Implementation Unit of South Sulawesi Province from June 2021 to September 2021. The study population was blood donors who met the donor selection requirements. The research samples were blood donors who qualified as routine and non-routine blood donors. For a total of 66 blood donors, namely 33 routine blood donors and 33 non-routine blood donors, the mean Ret-He of routine blood donors (33.6 pg) was lower than that of non-routine blood donors (35 pg). The Mann-Whitney test showed a significant difference between routine and non-routine blood donors (p=0.008). Ret-He levels in routine blood donors were significantly lower than in non-routine blood donors. This is because routine blood donors donate blood more frequently and regularly, affecting the iron reserves in the donor's body. The study showed that the Ret-He level of routine blood donors was lower than that of non-routine blood donors but was still within the normal values of Ret-He (normal value: 30.2 pg - 36.7 pg). Ret-He levels in routine blood donors were lower than non-routine blood donors but were still within the normal range.
{"title":"Analysis of Reticulocyte Hemoglobin Equivalent in Routine and Non-Routine Blood Donors in Makassar","authors":"Andy Inna Agustina, Samad R, Juliyani S, M. Arif","doi":"10.24293/ijcpml.v29i2.1984","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i2.1984","url":null,"abstract":"Blood donation often causes the depletion of iron stores in the body resulting in anemia. One of the markers to assess the hemoglobin content in reticulocytes is Ret-He. This study aims to analyze Ret-He in routine and non-routine blood donors. This was a cross-sectional study using primary data from routine and non-routine blood donors at the Blood Transfusion Technical Implementation Unit of South Sulawesi Province from June 2021 to September 2021. The study population was blood donors who met the donor selection requirements. The research samples were blood donors who qualified as routine and non-routine blood donors. For a total of 66 blood donors, namely 33 routine blood donors and 33 non-routine blood donors, the mean Ret-He of routine blood donors (33.6 pg) was lower than that of non-routine blood donors (35 pg). The Mann-Whitney test showed a significant difference between routine and non-routine blood donors (p=0.008). Ret-He levels in routine blood donors were significantly lower than in non-routine blood donors. This is because routine blood donors donate blood more frequently and regularly, affecting the iron reserves in the donor's body. The study showed that the Ret-He level of routine blood donors was lower than that of non-routine blood donors but was still within the normal values of Ret-He (normal value: 30.2 pg - 36.7 pg). Ret-He levels in routine blood donors were lower than non-routine blood donors but were still within the normal range.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122469148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.24293/ijcpml.v29i2.1830
Evelin Vianetha Prima Snak, I. Wande, Ni Nyoman Mahartini
Malaria is caused by Plasmodium, which is transmitted through the bite of infected female Anopheles mosquitoes. Plasmodium falciparum causes the most severe form of malaria and can be life-threatening. A 63-year-old male with decreased consciousness, fever, chills, vomiting, and joint pain. The patient works in the Ivory Coast, malaria-endemic areas. Physical examination found clouding of consciousness and jaundice. Laboratory examination results are leukocytosis with eosinophilia and thrombocytopenia, increased of Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), total bilirubin, direct and indirect bilirubin, Blood Urea Nitrogen (BUN), creatinine, decreased of estimated Glomerular Filtration Rate (e-GFR), decreased random plasma glucose. Urinalysis showed macroscopic hematuria, positive blood, and protein are found, and erythrocyte sediment is increased. A blood gas analysis examination revealed metabolic acidosis. Rapid Diagnostic Test (RDT) showed positive for Plasmodium falciparum. The blood smear showed leukocytosis with eosinophilia and thrombocytopenia and the ring-form trophozoites stage of Plasmodium falciparum. The definitive diagnosis of falciparum malaria is confirmed by microscopic peripheral blood smear and malaria RDT for antigen detection. An overall investigation concluded the patient diagnosed is severe falciparum malaria with various complications including hypoglycemia, jaundice, and acute kidney failure. The patient died on the first day after being treated in Sanglah Hospital, Denpasar.
{"title":"Severe Falciparum Malaria with Multiple Complications in Sanglah Hospital Denpasar","authors":"Evelin Vianetha Prima Snak, I. Wande, Ni Nyoman Mahartini","doi":"10.24293/ijcpml.v29i2.1830","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i2.1830","url":null,"abstract":"Malaria is caused by Plasmodium, which is transmitted through the bite of infected female Anopheles mosquitoes. Plasmodium falciparum causes the most severe form of malaria and can be life-threatening. A 63-year-old male with decreased consciousness, fever, chills, vomiting, and joint pain. The patient works in the Ivory Coast, malaria-endemic areas. Physical examination found clouding of consciousness and jaundice. Laboratory examination results are leukocytosis with eosinophilia and thrombocytopenia, increased of Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), total bilirubin, direct and indirect bilirubin, Blood Urea Nitrogen (BUN), creatinine, decreased of estimated Glomerular Filtration Rate (e-GFR), decreased random plasma glucose. Urinalysis showed macroscopic hematuria, positive blood, and protein are found, and erythrocyte sediment is increased. A blood gas analysis examination revealed metabolic acidosis. Rapid Diagnostic Test (RDT) showed positive for Plasmodium falciparum. The blood smear showed leukocytosis with eosinophilia and thrombocytopenia and the ring-form trophozoites stage of Plasmodium falciparum. The definitive diagnosis of falciparum malaria is confirmed by microscopic peripheral blood smear and malaria RDT for antigen detection. An overall investigation concluded the patient diagnosed is severe falciparum malaria with various complications including hypoglycemia, jaundice, and acute kidney failure. The patient died on the first day after being treated in Sanglah Hospital, Denpasar.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128955557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.24293/ijcpml.v29i2.2001
Ariosta Setyadi, Purwanto Adhipireno, L. Suromo, Charles Limantoro, A. Setiawan, Jessica Christanti, D. Retnoningrum, Nyoman Suci Widiastiti
Ubiquinone is an antioxidant that plays a role in preventing endothelial damage, thereby reducing the risk of myocardial infarction. In myocardial infarction, there is a decrease in ubiquinone levels and energy production in the form of ATP. Both stimulate anaerobic metabolism, which increases lactate dehydrogenase and lactate levels. This study aimed to analyze the correlation between ubiquinone levels, lactate dehydrogenase levels, and lactate levels in patients with acute myocardial infarction. This study was an analytical observational study with a cross-sectional approach. The normality of data was analyzed using the Kolmogorov-Smirnov test, and the correlation among variables was analyzed using the Spearman Rank test. The number of research subjects was 52, consisting of 25 research subjects with STEMI and 27 with NSTEMI. The median of ubiquinone, LDH, and lactate levels was 12.52 ng/mL (5.6–412.2); 310 U/L (3-1212); and 4 mmol/L (0.8 – 22), respectively. The correlation test results between ubiquinone levels with LDH levels obtained p=0.4 with r=-0.35; correlation test results between LDH levels and lactate levels obtained p=0.09, with r = -0.14. There was no correlation between acute myocardial infarct patients' ubiquinone levels, LDH levels, and lactate levels in AMI patients.
泛醌是一种抗氧化剂，在防止内皮损伤中起作用，从而降低心肌梗死的风险。在心肌梗死中，泛素水平和ATP形式的能量产生降低。两者都刺激无氧代谢，从而增加乳酸脱氢酶和乳酸水平。本研究旨在分析急性心肌梗死患者血清泛醌水平、乳酸脱氢酶水平和乳酸水平的相关性。本研究是一项横断面分析性观察研究。采用Kolmogorov-Smirnov检验分析数据的正态性，采用Spearman秩检验分析变量间的相关性。研究对象52人，其中STEMI 25人，NSTEMI 27人。泛醌、LDH和乳酸水平中位数为12.52 ng/mL (5.6-412.2);310 u / l (3-1212);和4 mmol/L(0.8 ~ 22)。泛醌水平与LDH水平的相关性检验结果为p=0.4, r=-0.35;LDH水平与乳酸水平的相关性检验结果p=0.09, r = -0.14。急性心肌梗死患者的泛素水平、LDH水平与AMI患者的乳酸水平无相关性。
{"title":"Correlation between Ubiquinone Levels, Lactate Dehydrogenase, and Lactate on Acute Myocardial Infarction","authors":"Ariosta Setyadi, Purwanto Adhipireno, L. Suromo, Charles Limantoro, A. Setiawan, Jessica Christanti, D. Retnoningrum, Nyoman Suci Widiastiti","doi":"10.24293/ijcpml.v29i2.2001","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i2.2001","url":null,"abstract":"Ubiquinone is an antioxidant that plays a role in preventing endothelial damage, thereby reducing the risk of myocardial infarction. In myocardial infarction, there is a decrease in ubiquinone levels and energy production in the form of ATP. Both stimulate anaerobic metabolism, which increases lactate dehydrogenase and lactate levels. This study aimed to analyze the correlation between ubiquinone levels, lactate dehydrogenase levels, and lactate levels in patients with acute myocardial infarction. This study was an analytical observational study with a cross-sectional approach. The normality of data was analyzed using the Kolmogorov-Smirnov test, and the correlation among variables was analyzed using the Spearman Rank test. The number of research subjects was 52, consisting of 25 research subjects with STEMI and 27 with NSTEMI. The median of ubiquinone, LDH, and lactate levels was 12.52 ng/mL (5.6–412.2); 310 U/L (3-1212); and 4 mmol/L (0.8 – 22), respectively. The correlation test results between ubiquinone levels with LDH levels obtained p=0.4 with r=-0.35; correlation test results between LDH levels and lactate levels obtained p=0.09, with r = -0.14. There was no correlation between acute myocardial infarct patients' ubiquinone levels, LDH levels, and lactate levels in AMI patients.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116621949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.24293/ijcpml.v29i2.1970
Maria Sekar Cahyaningrum, D. Ariningrum, W. Primaningtyas, T. Ardyanto
COVID-19 has been declared a global pandemic affecting many countries, including Indonesia. The worsening of COVID-19 patients may go unnoticed because one of the clinical manifestations of COVID-19 is silent hypoxia, where the patients’ blood oxygen saturation drops with no visible symptoms. Blood gas analysis is needed to detect silent hypoxia. This study aims to confirm whether blood gas analysis results correlate with the mortality of critical COVID-19 patients. This was a retrospective cohort study, the samples were taken from the medical records of critical COVID-19 patients from May 2020-July 2021. The variables observed were gender, age, comorbidities, oxygen saturation (SaO2), partial pressure of oxygen (PaO2), partial pressure of carbon dioxide (PaCO2), bicarbonate (HCO3-), and blood pH. Descriptive statistics, bivariate, and multivariate analyses were done. There were 210 samples, where 137 patients survived (65.24%) and 73 patients died (34.76%). The data analysis showed that comorbidities and blood pH significantly correlate with the mortality of critical COVID-19 patients, with p-values < 0.05; indicating that comorbidities and blood pH can be used as mortality predictors in critical COVID-19 patients. The RR for comorbidities and blood pH were 2.194 and 2>294, respectively, with CI 95% 1.202-4.833 for comorbidities and 1.151-5.295 for blood pH. The cut-off value used for blood pH was 7.310.
{"title":"Blood Gas Analysis as A Predictor of Mortality in Critical COVID-19 Patients in Dr. Moewardi Hospital Surakarta","authors":"Maria Sekar Cahyaningrum, D. Ariningrum, W. Primaningtyas, T. Ardyanto","doi":"10.24293/ijcpml.v29i2.1970","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i2.1970","url":null,"abstract":"COVID-19 has been declared a global pandemic affecting many countries, including Indonesia. The worsening of COVID-19 patients may go unnoticed because one of the clinical manifestations of COVID-19 is silent hypoxia, where the patients’ blood oxygen saturation drops with no visible symptoms. Blood gas analysis is needed to detect silent hypoxia. This study aims to confirm whether blood gas analysis results correlate with the mortality of critical COVID-19 patients. This was a retrospective cohort study, the samples were taken from the medical records of critical COVID-19 patients from May 2020-July 2021. The variables observed were gender, age, comorbidities, oxygen saturation (SaO2), partial pressure of oxygen (PaO2), partial pressure of carbon dioxide (PaCO2), bicarbonate (HCO3-), and blood pH. Descriptive statistics, bivariate, and multivariate analyses were done. There were 210 samples, where 137 patients survived (65.24%) and 73 patients died (34.76%). The data analysis showed that comorbidities and blood pH significantly correlate with the mortality of critical COVID-19 patients, with p-values < 0.05; indicating that comorbidities and blood pH can be used as mortality predictors in critical COVID-19 patients. The RR for comorbidities and blood pH were 2.194 and 2>294, respectively, with CI 95% 1.202-4.833 for comorbidities and 1.151-5.295 for blood pH. The cut-off value used for blood pH was 7.310.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126395857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.24293/ijcpml.v29i2.2049
L. B. Kurniawan, Martina Rentauli Sihombing, Endy Adnan, G. Soraya, T. Esa, Yuyun Widaningsih, U. Bahrun, M. Arif
Previous researches have demonstrated associations between high obesity indices with increased risk of metabolic and cardiovascular disorders. It has also been established that abnormalities of lipoprotein metabolism have an important role in atherogenesis and that non-menopausal females are protected from atherosclerotic cardiovascular events relative to menopausal females and males. This study aimed to investigate the relationship between obesity indices such as body mass index (BMI), waist circumference (WC), body fat percentage (BF), and visceral fat (VF) with apolipoprotein B/apolipoprotein A1 ratio in non-menopausal Indonesian adult females. A total of 75 non-menopausal Indonesian adult females were included as subjects in this cross-sectional study. Measured indices included BMI, WC, BF, and VF. Measurement of apolipoprotein B and A1 were performed by immunoturbidimetry, followed by calculation of the ratio. A cut-off value of 0.8 was used to define high apolipoprotein B/apolipoprotein A1 ratio. Apolipoprotein B/Apolipoprotein A1 ratio was significantly correlated with BMI (r=0.384, p=0.001), WC (r=0.363, p=0.001), BF (r=0.385, p=0.001), VF (r=0.380, p=0.001). The area under curve of BF (0.754) was slightly larger than BMI (0.722), VF (0.721), and WC (0.686) in predicting high apolipoprotein B/apolipoprotein A1 ratio. A positive significant correlation was observed between obesity indices with the apolipoprotein B/apolipoprotein A1 ratio. Obesity indices could be used to predict high apolipoprotein B/apolipoprotein A1 ratio.
{"title":"Obesity Indices Could Predict High Apolipoprotein B/Apolipoprotein A1 Ratio in Non-menopausal Indonesian Adult Females","authors":"L. B. Kurniawan, Martina Rentauli Sihombing, Endy Adnan, G. Soraya, T. Esa, Yuyun Widaningsih, U. Bahrun, M. Arif","doi":"10.24293/ijcpml.v29i2.2049","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i2.2049","url":null,"abstract":"Previous researches have demonstrated associations between high obesity indices with increased risk of metabolic and cardiovascular disorders. It has also been established that abnormalities of lipoprotein metabolism have an important role in atherogenesis and that non-menopausal females are protected from atherosclerotic cardiovascular events relative to menopausal females and males. This study aimed to investigate the relationship between obesity indices such as body mass index (BMI), waist circumference (WC), body fat percentage (BF), and visceral fat (VF) with apolipoprotein B/apolipoprotein A1 ratio in non-menopausal Indonesian adult females. A total of 75 non-menopausal Indonesian adult females were included as subjects in this cross-sectional study. Measured indices included BMI, WC, BF, and VF. Measurement of apolipoprotein B and A1 were performed by immunoturbidimetry, followed by calculation of the ratio. A cut-off value of 0.8 was used to define high apolipoprotein B/apolipoprotein A1 ratio. Apolipoprotein B/Apolipoprotein A1 ratio was significantly correlated with BMI (r=0.384, p=0.001), WC (r=0.363, p=0.001), BF (r=0.385, p=0.001), VF (r=0.380, p=0.001). The area under curve of BF (0.754) was slightly larger than BMI (0.722), VF (0.721), and WC (0.686) in predicting high apolipoprotein B/apolipoprotein A1 ratio. A positive significant correlation was observed between obesity indices with the apolipoprotein B/apolipoprotein A1 ratio. Obesity indices could be used to predict high apolipoprotein B/apolipoprotein A1 ratio.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122886040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.24293/ijcpml.v29i2.1991
Agnes Theresia Motulo, R. Muhiddin, Nadirah Rasyid Ridha, M. Arif, Agus Alim Abdullah
Assessment of treatment response is needed in the management of iron deficiency anemia (IDA). This study aims to analyze erythrocyte indices (MCH, MCV, MCHC) and Ret-He as indicators of IDA diagnosis and treatment response. A prospective cohort study in children ages 2-18 years old in orphanages throughout Makassar. Grouped into normal group and therapy group, consisting of IDA and iron deficiency groups. Elemental iron therapy 3mg/kg/day was given. Levels of MCV (fl), MCH (pg), MCHC (g/dL), and Ret-He (pg) were measured before and on the 8th day of therapy. The normality test of numerical variable data used the Kolmogorov-Smirnov test. The statistical test used the Mann-Whitney test, Wilcoxon Signed Rank test, and the Kruskal-Wallis test. Diagnostic value and cut-off determination using ROC curve analysis. Test results were significant if p<0.05. The sample consisted of 40 subjects each in both normal and therapy groups. The therapy group was divided into 7 IDA and 33 iron deficiency. ROC IDA curve on MCV obtained a cut-off of 76 fl, a sensitivity of 100%, a specificity of 95%, NPP of 77.8%, NPN of 100%, MCH obtained a cut-off of 25 pg, a sensitivity of 100%, 97.5% specificity of 97.5%, NPP of 87.5%, NPN of 100%, Ret-He obtained cut-off 29 pg, sensitivity of 100%, specificity of 95%, NPP of 77.5%, NPN of 100%. MCV levels increased by 7.3% (p<0.05) while Ret-He increased by 19.6% (p<0.05) after therapy. The ROC curve coordinate on IDA showed that cut-off levels of MCV 76 fl, MCH 25 pg, and Ret-He 29 pg provided optimal sensitivity and specificity. Increasing MCV after therapy described increasing levels in erythrocyte and hematocrit synthesis. Increasing Ret-He after therapy described improving erythropoiesis quality. MCV, MCH, and Ret-He as indicators of diagnosing IDA. MCV and Ret-He monitor the success of IDA treatment response.
{"title":"Analysis of Erythrocyte Indices and Reticulocyte Hemoglobin Equivalent in Iron Deficiency Anemia on Treatment","authors":"Agnes Theresia Motulo, R. Muhiddin, Nadirah Rasyid Ridha, M. Arif, Agus Alim Abdullah","doi":"10.24293/ijcpml.v29i2.1991","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i2.1991","url":null,"abstract":"Assessment of treatment response is needed in the management of iron deficiency anemia (IDA). This study aims to analyze erythrocyte indices (MCH, MCV, MCHC) and Ret-He as indicators of IDA diagnosis and treatment response. A prospective cohort study in children ages 2-18 years old in orphanages throughout Makassar. Grouped into normal group and therapy group, consisting of IDA and iron deficiency groups. Elemental iron therapy 3mg/kg/day was given. Levels of MCV (fl), MCH (pg), MCHC (g/dL), and Ret-He (pg) were measured before and on the 8th day of therapy. The normality test of numerical variable data used the Kolmogorov-Smirnov test. The statistical test used the Mann-Whitney test, Wilcoxon Signed Rank test, and the Kruskal-Wallis test. Diagnostic value and cut-off determination using ROC curve analysis. Test results were significant if p<0.05. The sample consisted of 40 subjects each in both normal and therapy groups. The therapy group was divided into 7 IDA and 33 iron deficiency. ROC IDA curve on MCV obtained a cut-off of 76 fl, a sensitivity of 100%, a specificity of 95%, NPP of 77.8%, NPN of 100%, MCH obtained a cut-off of 25 pg, a sensitivity of 100%, 97.5% specificity of 97.5%, NPP of 87.5%, NPN of 100%, Ret-He obtained cut-off 29 pg, sensitivity of 100%, specificity of 95%, NPP of 77.5%, NPN of 100%. MCV levels increased by 7.3% (p<0.05) while Ret-He increased by 19.6% (p<0.05) after therapy. The ROC curve coordinate on IDA showed that cut-off levels of MCV 76 fl, MCH 25 pg, and Ret-He 29 pg provided optimal sensitivity and specificity. Increasing MCV after therapy described increasing levels in erythrocyte and hematocrit synthesis. Increasing Ret-He after therapy described improving erythropoiesis quality. MCV, MCH, and Ret-He as indicators of diagnosing IDA. MCV and Ret-He monitor the success of IDA treatment response.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114885779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-05DOI: 10.24293/ijcpml.v29i2.1971
Rafika Ulandari, L. B. Kurniawan, Nurahmi Nurahmi, D. Muhadi
Patients with type 2 diabetes mellitus (T2DM) have increased followed by complications including diabetic foot ulcer. Systemic inflammatory conditions in T2DM with diabetic foot ulcers can be assessed by inflammatory markers. Neutrophil Lymphocyte Ratio (NLR) is a good indicator of systemic inflammatory conditions. A retrospective study of the medical record at Dr. Wahidin Sudirohusodo Hospital, Makassar from September 2019 – September 2021 involved 120 patients consisting of 60 patients for each group of T2DM with and without diabetic foot ulcers. Leukocytes, neutrophils, lymphocytes, and NLR based on routine blood results using the flow cytometry method. Mann-Whitney test was used for comparison between the two groups on NLR and Kruskal-Wallist test was used for the relationship between NLR and Wagner classification. There was a significant difference in leukocytes, neutrophils, lymphocytes, and NLR in T2DM patients with diabetic foot ulcers compared to those without 16.2±8.6 and 9.8±4.2 103/µL (p<0.001); 13.3±8.4 and 5.0±3.8 103/µL(p<0.001); 1.6±1.7 and 2.5±2.5 103/µL(p<0.001); 10.0±10.1 and 3.5±4.5, respectively. The relationship between the NLR and Wagner classification was the highest at Wagner grade 5 (12.87±5.0) and the lowest was at Wagner grade 2 (6.18±7.83) with significant statistical test results (p<0.037). There was increasing NLR in T2DM with diabetic foot ulcers due to systemic inflammation. The NLR integrates different immune pathways, such as neutrophils as an inflammatory response and lymphocytes controlling the inflammatory response. Lymphocytes count and NLR level on T2DM with diabetic foot ulcer were higher than those without diabetic foot ulcer.
{"title":"Analysis of NLR in Type 2 Diabetes Mellitus with and without Diabetic Foot Ulcer","authors":"Rafika Ulandari, L. B. Kurniawan, Nurahmi Nurahmi, D. Muhadi","doi":"10.24293/ijcpml.v29i2.1971","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i2.1971","url":null,"abstract":"Patients with type 2 diabetes mellitus (T2DM) have increased followed by complications including diabetic foot ulcer. Systemic inflammatory conditions in T2DM with diabetic foot ulcers can be assessed by inflammatory markers. Neutrophil Lymphocyte Ratio (NLR) is a good indicator of systemic inflammatory conditions. A retrospective study of the medical record at Dr. Wahidin Sudirohusodo Hospital, Makassar from September 2019 – September 2021 involved 120 patients consisting of 60 patients for each group of T2DM with and without diabetic foot ulcers. Leukocytes, neutrophils, lymphocytes, and NLR based on routine blood results using the flow cytometry method. Mann-Whitney test was used for comparison between the two groups on NLR and Kruskal-Wallist test was used for the relationship between NLR and Wagner classification. There was a significant difference in leukocytes, neutrophils, lymphocytes, and NLR in T2DM patients with diabetic foot ulcers compared to those without 16.2±8.6 and 9.8±4.2 103/µL (p<0.001); 13.3±8.4 and 5.0±3.8 103/µL(p<0.001); 1.6±1.7 and 2.5±2.5 103/µL(p<0.001); 10.0±10.1 and 3.5±4.5, respectively. The relationship between the NLR and Wagner classification was the highest at Wagner grade 5 (12.87±5.0) and the lowest was at Wagner grade 2 (6.18±7.83) with significant statistical test results (p<0.037). There was increasing NLR in T2DM with diabetic foot ulcers due to systemic inflammation. The NLR integrates different immune pathways, such as neutrophils as an inflammatory response and lymphocytes controlling the inflammatory response. Lymphocytes count and NLR level on T2DM with diabetic foot ulcer were higher than those without diabetic foot ulcer.\u0000 ","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128575694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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