Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.1835
Ni Komang Krisnawati, N. Mulyantari, Ni Nyoman Mahartini
Herpes Simplex Virus (HSV) is a common cause of infectious encephalitis in children, which can lead to severe neurological sequels. There is a new perspective suggesting that herpes simplex encephalitis plays an important role in triggering the synthesis of NMDA receptor antibodies. There have been no data on the incidence of anti-NMDA receptor encephalitis in Indonesia. Herpes simplex encephalitis was found in these patients as a risk factor for anti-NMDA receptor encephalitis. A 9-year-old male patient complained of seizures such as jerking of the right hand and smacking of the lips along with fever for 4 days before being admitted to the hospital. Complaints began with slurred speech and t inability of fingers to hold objects for the previous 8 days. From the physical examination, it was reported that the patient looked seriously ill, blood pressure was 110/80 mmHg, pulse was 117 beats per minute, the temperature was 38.5 C, and respiratory rate was 24 times per minute. Another physical examination was within normal limits. The results of a complete blood count at the time of initial treatment of patients showed mild leukocytosis due to a mild increase in monocytes, whereas blood gas and electrolyte analysis showed hypoventilation at the beginning of hospitalization in which mixed acid-base disturbance, respiratory acidosis, and metabolic alkalosis occurred. Analysis of cerebrospinal fluid in these patients showed mononuclear pleocytosis and the results of immunoserological test in serum indicated HSV1 and HSV2 infection and detected anti-NMDA receptor. Both clinical and laboratory findings in this study supported encephalitis. Herpes simplex virus infection can be a risk factor for patients suffering from anti-NMDA receptor encephalitis.
{"title":"Anti- N-MethyL-D-Aspartate Receptor Encephalitis in Children Infected with Herpes Simplex","authors":"Ni Komang Krisnawati, N. Mulyantari, Ni Nyoman Mahartini","doi":"10.24293/ijcpml.v29i3.1835","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.1835","url":null,"abstract":"Herpes Simplex Virus (HSV) is a common cause of infectious encephalitis in children, which can lead to severe neurological sequels. There is a new perspective suggesting that herpes simplex encephalitis plays an important role in triggering the synthesis of NMDA receptor antibodies. There have been no data on the incidence of anti-NMDA receptor encephalitis in Indonesia. Herpes simplex encephalitis was found in these patients as a risk factor for anti-NMDA receptor encephalitis. A 9-year-old male patient complained of seizures such as jerking of the right hand and smacking of the lips along with fever for 4 days before being admitted to the hospital. Complaints began with slurred speech and t inability of fingers to hold objects for the previous 8 days. From the physical examination, it was reported that the patient looked seriously ill, blood pressure was 110/80 mmHg, pulse was 117 beats per minute, the temperature was 38.5 C, and respiratory rate was 24 times per minute.\u0000Another physical examination was within normal limits. The results of a complete blood count at the time of initial treatment of patients showed mild leukocytosis due to a mild increase in monocytes, whereas blood gas and electrolyte analysis showed hypoventilation at the beginning of hospitalization in which mixed acid-base disturbance, respiratory acidosis, and metabolic alkalosis occurred. Analysis of cerebrospinal fluid in these patients showed mononuclear pleocytosis and the results of immunoserological test in serum indicated HSV1 and HSV2 infection and detected anti-NMDA receptor. Both clinical and laboratory findings in this study supported encephalitis. Herpes simplex virus infection can be a risk factor for patients suffering from anti-NMDA receptor encephalitis.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124439957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.2018
A. Indrati, Yehezkiel Yonathan, H. Hendro, Lazuardi Dwipa, C. Kartasasmita
Geriatric anemia can cause many problems and is a major health condition worldwide and in Indonesia. It is important to know the characteristics and prevalence of anemia among geriatrics in Indonesia, especially in West Java. By using simple hematology parameters to detect anemia in geriatrics, and then giving adequate treatment, morbidity, and mortality due to anemia may decrease in geriatrics. This study aimed to identify the type of anemia, etiology, age, gender, and prevalence of elderly patients with anemia in West Java. This was a cross-sectional study. Patients above 59 years old attending the geriatric clinic in Hasan Sadikin General Hospital and the geriatric community was included in this study to be evaluated for anemia, the characteristics of anemia (normocytic, microcytic, and macrocytic) based on MCV and MCHC, age group (<=65 and > 65 year old), and gender. Among 247 elderly patients, anemia was found in 33 patients (13.64%) and mostly found in females. Mostly represented as normochromic normocytic anemia (78.8%) and microcytic hypochromic anemia (21.2%), respectively. Individuals older than 65 years old were mostly presented with microcytic hypochromic anemia (66.7%). Meanwhile, normocytic normochromic anemia was more commonly found in individuals below 65 years old (12.1%). Anemia was commonly found in people older than 65 years old. The most common type of anemia in geriatrics in West Java was normocytic normochromic. Females experience anemia more than males.
{"title":"Characteristics of Anemia in the Elderly: Hospital-Based Study in West Java","authors":"A. Indrati, Yehezkiel Yonathan, H. Hendro, Lazuardi Dwipa, C. Kartasasmita","doi":"10.24293/ijcpml.v29i3.2018","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.2018","url":null,"abstract":"Geriatric anemia can cause many problems and is a major health condition worldwide and in Indonesia. It is important to know the characteristics and prevalence of anemia among geriatrics in Indonesia, especially in West Java. By using simple hematology parameters to detect anemia in geriatrics, and then giving adequate treatment, morbidity, and mortality due to anemia may decrease in geriatrics. This study aimed to identify the type of anemia, etiology, age, gender, and prevalence of elderly patients with anemia in West Java. This was a cross-sectional study. Patients above 59 years old attending the geriatric clinic in Hasan Sadikin General Hospital and the geriatric community was included in this study to be evaluated for anemia, the characteristics of anemia (normocytic, microcytic, and macrocytic) based on MCV and MCHC, age group (<=65 and > 65 year old), and gender. Among 247 elderly patients, anemia was found in 33 patients (13.64%) and mostly found in females. Mostly represented as normochromic normocytic anemia (78.8%) and microcytic hypochromic anemia (21.2%), respectively. Individuals older than 65 years old were mostly presented with microcytic hypochromic anemia (66.7%). Meanwhile, normocytic normochromic anemia was more commonly found in individuals below 65 years old (12.1%). Anemia was commonly found in people older than 65 years old. The most common type of anemia in geriatrics in West Java was normocytic normochromic. Females experience anemia more than males.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133792744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.2031
I. Timan, A. Aryati
Every clinical laboratory has to establish its reference ranges for every parameter analyzed. These reference values are intervals considered normal in a healthy person's physiological condition. It will be used by the clinician or other health professionals to interpret the laboratory test results of the patient for making diagnostic decisions, monitoring patient therapy, and predicting the prognosis and it is also used in epidemiology studies. A reference range is defined as an interval in which 95% of a reference population’s values fall. It is very crucial to establish reference intervals for the local population as it is sometimes affected by ethnicity, nutrition, food habits, and economic and other local conditions. Hematology is the most common test performed in a clinical laboratory, and the most used parameter by the clinician including pediatricians. There up to now no well-established reference interval for hematological parameters in pediatrics and children in Indonesia. The reference interval used for hematology parameters in Indonesia is based on reference intervals from other countries or the manufacturer of the hematology analyzers used in a particular laboratory. Establishing a population-specific hematology reference interval is very difficult for pediatrics and children, so it is rarely performed. This study aims to compile the results of hematology parameters from Indonesian children to establish an Indonesian reference value for hematology. The method used was compiling data for the hematology parameter intervals based on studies performed in the Indonesian population and presented as 95% confidence intervals. A pediatric reference range for several hematology parameters has been established based on 3 groups of age in Indonesian children.
{"title":"Hematology Reference Values in Indonesian Children","authors":"I. Timan, A. Aryati","doi":"10.24293/ijcpml.v29i3.2031","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.2031","url":null,"abstract":"Every clinical laboratory has to establish its reference ranges for every parameter analyzed. These reference values are intervals considered normal in a healthy person's physiological condition. It will be used by the clinician or other health professionals to interpret the laboratory test results of the patient for making diagnostic decisions, monitoring patient therapy, and predicting the prognosis and it is also used in epidemiology studies. A reference range is defined as an interval in which 95% of a reference population’s values fall. It is very crucial to establish reference intervals for the local population as it is sometimes affected by ethnicity, nutrition, food habits, and economic and other local conditions. Hematology is the most common test performed in a clinical laboratory, and the most used parameter by the clinician including pediatricians. There up to now no well-established reference interval for hematological parameters in pediatrics and children in Indonesia. The reference interval used for hematology parameters in Indonesia is based on reference intervals from other countries or the manufacturer of the hematology analyzers used in a particular laboratory. Establishing a population-specific hematology reference interval is very difficult for pediatrics and children, so it is rarely performed. This study aims to compile the results of hematology parameters from Indonesian children to establish an Indonesian reference value for hematology. The method used was compiling data for the hematology parameter intervals based on studies performed in the Indonesian population and presented as 95% confidence intervals. A pediatric reference range for several hematology parameters has been established based on 3 groups of age in Indonesian children.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"52 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116594939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.2020
M. Aditya, B. Senaputra, Rina A. Sidharta, Lusi Oka
Hepatitis B Virus (HBV) infection causes inflammation of the liver, which has a high prevalence in both Indonesia and the world. Serum HBV deoxyribonucleic acid (DNA) is important in determining the initiation therapy for Chronic Hepatitis B (CHB) patients. However, it has several limitations. Precore protein 22 kilodalton (p22cr) is synthesized from the HBV gene in hepatocytes, representing covalently closed circle (ccc) DNA. This study aimed to analyze the diagnostic performance of p22cr levels on HBV DNA in CHB patients. An observational analytic study with a cross-sectional approach was conducted on 83 CHB patients who were examined at the Clinical Pathology Laboratory of Dr. Moewardi General Academic Hospital in December 2020. Blood plasma samples were taken for HBV DNA and p22cr examination by using Polymerase Chain Reaction (PCR) and Enzyme-Linked Immunosorbent Assay (ELISA), respectively. The cut-off level of p22cr was determined by the Receiver Operating Curve (ROC) with the widest area Under the Curve (AUC). Sensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV), Positive Likelihood Ratio (PLR), Negative Likelihood Ratio (NLR), and accuracy were calculated for the diagnostic performance of p22cr. The cut-off point of p22cr on HBV DNA > 20,000 IU/mL was 7.440 ng/mL with AUC 0.693 (p=0.003). The diagnostic performance of p22cr levels on HBV DNA obtained 44.44% sensitivity, 82.98% specificity, 66.67% PPV, 66.10% NPV, 2.61 PLR, 0.67 NLR, and 66.27% accuracy. P22cr level has a good specificity so it can be an alternative examination of HBV DNA in making decisions on therapy in patients with chronic hepatitis B. Further research needs to be done using HBcrAg and excluding elderly patients.
{"title":"Diagnostic Performance of Precore Protein 22 Kilodalton Levels of HBV DNA in Chronic Hepatitis B Patients","authors":"M. Aditya, B. Senaputra, Rina A. Sidharta, Lusi Oka","doi":"10.24293/ijcpml.v29i3.2020","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.2020","url":null,"abstract":"Hepatitis B Virus (HBV) infection causes inflammation of the liver, which has a high prevalence in both Indonesia and the world. Serum HBV deoxyribonucleic acid (DNA) is important in determining the initiation therapy for Chronic Hepatitis B (CHB) patients. However, it has several limitations. Precore protein 22 kilodalton (p22cr) is synthesized from the HBV gene in hepatocytes, representing covalently closed circle (ccc) DNA. This study aimed to analyze the diagnostic performance of p22cr levels on HBV DNA in CHB patients. An observational analytic study with a cross-sectional approach was conducted on 83 CHB patients who were examined at the Clinical Pathology Laboratory of Dr. Moewardi General Academic Hospital in December 2020. Blood plasma samples were taken for HBV DNA and p22cr examination by using Polymerase Chain Reaction (PCR) and Enzyme-Linked Immunosorbent Assay (ELISA), respectively. The cut-off level of p22cr was determined by the Receiver Operating Curve (ROC) with the widest area Under the Curve (AUC). Sensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV), Positive Likelihood Ratio (PLR), Negative Likelihood Ratio (NLR), and accuracy were calculated for the diagnostic performance of p22cr. The cut-off point of p22cr on HBV DNA > 20,000 IU/mL was 7.440 ng/mL with AUC 0.693 (p=0.003). The diagnostic performance of p22cr levels on HBV DNA obtained 44.44% sensitivity, 82.98% specificity, 66.67% PPV, 66.10% NPV, 2.61 PLR, 0.67 NLR, and 66.27% accuracy. P22cr level has a good specificity so it can be an alternative examination of HBV DNA in making decisions on therapy in patients with chronic hepatitis B. Further research needs to be done using HBcrAg and excluding elderly patients.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123675096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.2048
N. Nurjannah, Nurahmi Nurahmi, L. B. Kurniawan
There are more than 3.4 million deaths every year in the world due to overweight and obesity. Obesity is a chronic proinflammatory condition marked by increased lipid and adipose tissues, leading to ectopic fat accumulation with increased levels of proinflammatory cytokines. Obesity causes a progressive increase in galectin-3 expression, especially in visceral and subcutaneous adipose tissue in experimental animals. Galectin-3 is upregulated in obesity and is defined as a proinflammatory molecule that can cause insulin resistance. This study aims to analyze differences in galectin-3 levels between obese and non-obese subjects. This was a cross-sectional study, using a total of 80 subjects. The study was conducted throughout August 2022. The samples were grouped into obese and non-obese based on Body Mass Index (BMI). The galectin-3 measurement used the Enzyme-Linked Immunosorbent Assay (ELISA) method. Statistical tests used the Mann-Whitney test and Spearmen rho; the test results were significant if the p-value <0.05. The samples were divided into obese and non-obese groups, each group had 40 people. Galectin-3 levels in the obese group were 0.9±0.36 ng/mL significantly higher than the non-obese group’s 0.43±0.11 ng/mL, (p<0.001). There was a strong positive correlation between levels of galectin-3 and BMI, (r=0.866, p<0.001). There is a positive correlation between serum galectin-3 levels and BMI. The greater value of the BMI, the higher levels of galectin-3.
{"title":"Correlation Analysis of Galectin-3 Serum Level in Obesity with and without Obesity","authors":"N. Nurjannah, Nurahmi Nurahmi, L. B. Kurniawan","doi":"10.24293/ijcpml.v29i3.2048","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.2048","url":null,"abstract":"There are more than 3.4 million deaths every year in the world due to overweight and obesity. Obesity is a chronic proinflammatory condition marked by increased lipid and adipose tissues, leading to ectopic fat accumulation with increased levels of proinflammatory cytokines. Obesity causes a progressive increase in galectin-3 expression, especially in visceral and subcutaneous adipose tissue in experimental animals. Galectin-3 is upregulated in obesity and is defined as a proinflammatory molecule that can cause insulin resistance. This study aims to analyze differences in galectin-3 levels between obese and non-obese subjects. This was a cross-sectional study, using a total of 80 subjects. The study was conducted throughout August 2022. The samples were grouped into obese and non-obese based on Body Mass Index (BMI). The galectin-3 measurement used the Enzyme-Linked Immunosorbent Assay (ELISA) method. Statistical tests used the Mann-Whitney test and Spearmen rho; the test results were significant if the p-value <0.05. The samples were divided into obese and non-obese groups, each group had 40 people. Galectin-3 levels in the obese group were 0.9±0.36 ng/mL significantly higher than the non-obese group’s 0.43±0.11 ng/mL, (p<0.001). There was a strong positive correlation between levels of galectin-3 and BMI, (r=0.866, p<0.001). There is a positive correlation between serum galectin-3 levels and BMI. The greater value of the BMI, the higher levels of galectin-3.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134353117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.2151
Alya Rahmaditya Arfan, L. B. Kurniawan, M. Arif, Husaini Umar, Nurahmi, Burhanuddin Bahar
Type 2 Diabetes Mellitus (T2DM) is characterized by the reduced ability of insulin responses, leading to difficulty in processing blood sugar. It is caused by a combination of two main factors: damaged insulin secretion by pancreatic beta-cells and the inability of insulin-sensitive tissues to respond to insulin. Blood sugar monitoring in T2DM is done by measuring glycated hemoglobin or Hemoglobin A1c (HbA1c). Insulin Like Growth Factor-1 (IGF-1) is the primary mediator of growth hormone known to play a pivotal biological role in growth and metabolism. This study aims to analyze the relationship between HbA1c levels and serum IGF-1 levels in T2DM patients. The method used was observational analytic with a cross-sectional design. There were 60 T2DM patients involved as research subjects consisting of 26 males and 34 females. HbA1c examination was carried out using the Boronete Affinity Assay, while IGF-1 examination was performed using the Enzyme-Linked Immunosorbent Assay (ELISA) sandwich method. The statistical analysis results showed that the average value and standard deviation of serum IGF-1 levels in controlled T2DM was higher (5740.23±4320.60 pg/mL) than that of uncontrolled T2DM (4843.18±3375.63 pg/mL), showed no significant difference (p=0.462) and no correlation between HbA1c and serum IGF-1 levels in T2DM subjects (r=-0.005 p=0.972). It was concluded that there was no significant relationship between HbA1c levels and serum IGF-1 levels in patients with T2DM.
2型糖尿病(T2DM)的特点是胰岛素反应能力降低，导致血糖处理困难。它是由两个主要因素共同引起的:胰腺β细胞的胰岛素分泌受损，以及胰岛素敏感组织无法对胰岛素作出反应。T2DM患者的血糖监测是通过测量糖化血红蛋白或糖化血红蛋白(HbA1c)来完成的。胰岛素样生长因子-1 (Insulin Like Growth Factor-1, IGF-1)是生长激素的主要调节因子，在生长和代谢中起着关键的生物学作用。本研究旨在分析T2DM患者HbA1c水平与血清IGF-1水平的关系。使用的方法是观察分析与横断面设计。研究对象为60例T2DM患者，其中男性26例，女性34例。HbA1c检测采用Boronete亲和试验，IGF-1检测采用酶联免疫吸附试验(ELISA)夹心法。统计分析结果显示，控制T2DM患者血清IGF-1水平的平均值(5740.23±4320.60 pg/mL)高于未控制T2DM患者(4843.18±3375.63 pg/mL)，差异无统计学意义(p=0.462)， HbA1c与T2DM患者血清IGF-1水平无相关性(r=-0.005 p=0.972)。结论:T2DM患者HbA1c水平与血清IGF-1水平无显著相关性。
{"title":"Analysis of the Relationship between HbA1c and Serum IGF-1 Levels in Patients with T2DM","authors":"Alya Rahmaditya Arfan, L. B. Kurniawan, M. Arif, Husaini Umar, Nurahmi, Burhanuddin Bahar","doi":"10.24293/ijcpml.v29i3.2151","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.2151","url":null,"abstract":"Type 2 Diabetes Mellitus (T2DM) is characterized by the reduced ability of insulin responses, leading to difficulty in processing blood sugar. It is caused by a combination of two main factors: damaged insulin secretion by pancreatic beta-cells and the inability of insulin-sensitive tissues to respond to insulin. Blood sugar monitoring in T2DM is done by measuring glycated hemoglobin or Hemoglobin A1c (HbA1c). Insulin Like Growth Factor-1 (IGF-1) is the primary mediator of growth hormone known to play a pivotal biological role in growth and metabolism. This study aims to analyze the relationship between HbA1c levels and serum IGF-1 levels in T2DM patients. The method used was observational analytic with a cross-sectional design. There were 60 T2DM patients involved as research subjects consisting of 26 males and 34 females. HbA1c examination was carried out using the Boronete Affinity Assay, while IGF-1 examination was performed using the Enzyme-Linked Immunosorbent Assay (ELISA) sandwich method. The statistical analysis results showed that the average value and standard deviation of serum IGF-1 levels in controlled T2DM was higher (5740.23±4320.60 pg/mL) than that of uncontrolled T2DM (4843.18±3375.63 pg/mL), showed no significant difference (p=0.462) and no correlation between HbA1c and serum IGF-1 levels in T2DM subjects (r=-0.005 p=0.972). It was concluded that there was no significant relationship between HbA1c levels and serum IGF-1 levels in patients with T2DM.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126176512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.2047
Patachna Junita, R. Pakasi, L. B. Kurniawan
Obesity is excessive body fat and was associated with the importance of metabolic and endocrine problems in somatotropic secretion in functional obesity. Ghrelin is an acylated peptide hormone produced by the stomach, which is a mediator of the growth hormone secretory receptor. The activity of ghrelin stimulates the release of growth hormone, and appetite and stimulates the metabolism of carbohydrates. Circulating ghrelin levels in healthy people increase during fasting and decrease after meals. This study aims to analyze the difference in ghrelin levels among obese and non-obese subjects. A cross-sectional design research was conducted in August 2022. The samples consisted of obese and non-obese subjects based on Body Mass Index (BMI). Ghrelin levels were measured using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The data were statistically analyzed using the Mann-Whitney and Spearman tests. p<0.05 was reported significant. The samples consisted of 80 obese and non-obese subjects, 39 males and 41 females. There was no difference in ghrelin serum levels in the obese group (1.45±2 ng/mL) compared to the non-obese group (0.67±0.25 ng/mL) with p =0.233 (p>0.05). There was a positive correlation between ghrelin levels and BMI (r=0.247). There was no difference in ghrelin levels between the obese group and the non-obese group, and there was a positive correlation between ghrelin levels and BMI. A higher BMI would lead to higher ghrelin levels.
{"title":"Analysis of Serum Ghrelin Levels and BMI in Obese and Non-Obese Subjects","authors":"Patachna Junita, R. Pakasi, L. B. Kurniawan","doi":"10.24293/ijcpml.v29i3.2047","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.2047","url":null,"abstract":"Obesity is excessive body fat and was associated with the importance of metabolic and endocrine problems in somatotropic secretion in functional obesity. Ghrelin is an acylated peptide hormone produced by the stomach, which is a mediator of the growth hormone secretory receptor. The activity of ghrelin stimulates the release of growth hormone, and appetite and stimulates the metabolism of carbohydrates. Circulating ghrelin levels in healthy people increase during fasting and decrease after meals. This study aims to analyze the difference in ghrelin levels among obese and non-obese subjects. A cross-sectional design research was conducted in August 2022. The samples consisted of obese and non-obese subjects based on Body Mass Index (BMI). Ghrelin levels were measured using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The data were statistically analyzed using the Mann-Whitney and Spearman tests. p<0.05 was reported significant. The samples consisted of 80 obese and non-obese subjects, 39 males and 41 females. There was no difference in ghrelin serum levels in the obese group (1.45±2 ng/mL) compared to the non-obese group (0.67±0.25 ng/mL) with p =0.233 (p>0.05). There was a positive correlation between ghrelin levels and BMI (r=0.247). There was no difference in ghrelin levels between the obese group and the non-obese group, and there was a positive correlation between ghrelin levels and BMI. A higher BMI would lead to higher ghrelin levels.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123545666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.2019
D. Anggraini, Prima Adelin
Chronic Kidney Disease (CKD) is a very common clinical problem in elderly patients and is associated with increased morbidity and mortality. The proportion of the elderly is predicted to further rise to 20% by the year 2030 caused by the demographic change from a pattern of high birth rates and high mortality to low birth rates. Delayed mortality has contributed to the rise in the elderly population. The aging process involves physiological and nutritional changes that will affect the nutritional status of the elderly, leading to malnutrition and overweight. Aging is associated with considerable changes in body composition, higher BMI is associated with improved survival in patients with CKD and very low BMI levels have been consistently associated with high all-cause mortality in the elderly. The changes in Body Fat Percentage (BFP) in the elderly will affect the estimated Glomerular Filtration Rate (eGFR) in the elderly. This study aimed to determine the correlation between anthropometric measurement and kidney function in the elderly population. this research is an analytic observational study with a cross-sectional design, which involved 42 elderly people with age > 60 years in Guguak District, West Sumatera, Indonesia. Anthropometric measurement using the Waist-to-Height-Ratio (WHtR) method, Waist-to-Hip Ratio (WHR), Waist Circumference (WC), and Body Mass Index (BMI) and eGFR were calculated using the Cockcroft and Gault formula. The results of this study showed that the mean age of the elderly was 68±7.58 years, which consisted of 29.3% male and 70.7% female. The Pearson correlation test between WHR and eGFR obtained p<0.05 with r= 0.439 and the Pearson’s correlation test between BMI and eGFR obtained p<0.05 with r= 0.425. There was a moderate correlation between WHR with eGFR and BMI with eGFR in the elderly population.
{"title":"Correlation between Anthropometric Measurement and Kidney Function in the Elderly to Detection of Chronic Kidney Disease","authors":"D. Anggraini, Prima Adelin","doi":"10.24293/ijcpml.v29i3.2019","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.2019","url":null,"abstract":"Chronic Kidney Disease (CKD) is a very common clinical problem in elderly patients and is associated with increased morbidity and mortality. The proportion of the elderly is predicted to further rise to 20% by the year 2030 caused by the demographic change from a pattern of high birth rates and high mortality to low birth rates. Delayed mortality has contributed to the rise in the elderly population. The aging process involves physiological and nutritional changes that will affect the nutritional status of the elderly, leading to malnutrition and overweight. Aging is associated with considerable changes in body composition, higher BMI is associated with improved survival in patients with CKD and very low BMI levels have been consistently associated with high all-cause mortality in the elderly. The changes in Body Fat Percentage (BFP) in the elderly will affect the estimated Glomerular Filtration Rate (eGFR) in the elderly. This study aimed to determine the correlation between anthropometric measurement and kidney function in the elderly population. this research is an analytic observational study with a cross-sectional design, which involved 42 elderly people with age > 60 years in Guguak District, West Sumatera, Indonesia. Anthropometric measurement using the Waist-to-Height-Ratio (WHtR) method, Waist-to-Hip Ratio (WHR), Waist Circumference (WC), and Body Mass Index (BMI) and eGFR were calculated using the Cockcroft and Gault formula. The results of this study showed that the mean age of the elderly was 68±7.58 years, which consisted of 29.3% male and 70.7% female. The Pearson correlation test between WHR and eGFR obtained p<0.05 with r= 0.439 and the Pearson’s correlation test between BMI and eGFR obtained p<0.05 with r= 0.425. There was a moderate correlation between WHR with eGFR and BMI with eGFR in the elderly population.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132722623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.2022
Wita Prominensa, Rina A. Sidharta, Lusi Oka, JB Wardhani, MI Suparyatmo, Diah Pramudianti
Chronic Hepatitis B (CHB) is a concern for Chronic Liver Disease (CLD) and causes a 74% mortality rate in Asia Pacific. World Health Organization (WHO) showed Indonesia is the highest second country of Hepatitis B (HB) in the South East Asian Region, Central Java is the highest in Java and Dr. Moewardi Hospital (RSDM) Surakarta in 2019 increased to 15%. Liver biopsy is fibrosis gold standard staging. It has limitations and requires invasive procedure pain in 40% of patients. This study aimed to determine M2BPGi diagnostic test against to transient elastography (TE) Fibroscan® (sensitivity 85.7%, specificity 81.6%) as a predictor of significant liver fibrosis of CHB in RSDM. Fibroscan® examination was performed on patients diagnosed with CHB by a clinician performed at the endoscopy department of RSDM, whereas laboratory tests were carried out from December 2020 to January 2021. Plasma M2BPG-I cut-off value was determined using Receiving Operating Characteristic (ROC) curve, M2BPGi levels were measured sandwich ELISA using spectrophotometry at a wavelength of 450 ± 2 nm. A total of 70 subjects was divided into 35 subjects with significant and 35 subjects with non-significant fibrosis. The results of the statistical calculation showed that plasma M2BPGi levels had a cut-off of 12.939 ng/mL (mean value of 17.841 ng/mL with significant fibrosis at 16.74 ng/mL and non-significant fibrosis at 10.14 ng/mL) had a moderate performance as a marker of liver fibrosis in CHB (71.4% sensitivity; 68.6% specificity; 69.4% PPV; 70.6% NPV and PLR 2.273), NR 0.417 with AUC of 0.727, CI 96% (0.681-0.0906). M2BPGi plasma levels at a cut-off of 12.939 ng/mL had a moderate performance as a predictor of significant liver fibrosis in chronic hepatitis B patients.
{"title":"Diagnostic Performance of Mac 2–Binding Protein Glycosylation Isomer in Chronic Hepatitis B","authors":"Wita Prominensa, Rina A. Sidharta, Lusi Oka, JB Wardhani, MI Suparyatmo, Diah Pramudianti","doi":"10.24293/ijcpml.v29i3.2022","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.2022","url":null,"abstract":"Chronic Hepatitis B (CHB) is a concern for Chronic Liver Disease (CLD) and causes a 74% mortality rate in Asia Pacific. World Health Organization (WHO) showed Indonesia is the highest second country of Hepatitis B (HB) in the South East Asian Region, Central Java is the highest in Java and Dr. Moewardi Hospital (RSDM) Surakarta in 2019 increased to 15%. Liver biopsy is fibrosis gold standard staging. It has limitations and requires invasive procedure pain in 40% of patients. This study aimed to determine M2BPGi diagnostic test against to transient elastography (TE) Fibroscan® (sensitivity 85.7%, specificity 81.6%) as a predictor of significant liver fibrosis of CHB in RSDM. Fibroscan® examination was performed on patients diagnosed with CHB by a clinician performed at the endoscopy department of RSDM, whereas laboratory tests were carried out from December 2020 to January 2021. Plasma M2BPG-I cut-off value was determined using Receiving Operating Characteristic (ROC) curve, M2BPGi levels were measured sandwich ELISA using spectrophotometry at a wavelength of 450 ± 2 nm. A total of 70 subjects was divided into 35 subjects with significant and 35 subjects with non-significant fibrosis. The results of the statistical calculation showed that plasma M2BPGi levels had a cut-off of 12.939 ng/mL (mean value of 17.841 ng/mL with significant fibrosis at 16.74 ng/mL and non-significant fibrosis at 10.14 ng/mL) had a moderate performance as a marker of liver fibrosis in CHB (71.4% sensitivity; 68.6% specificity; 69.4% PPV; 70.6% NPV and PLR 2.273), NR 0.417 with AUC of 0.727, CI 96% (0.681-0.0906). M2BPGi plasma levels at a cut-off of 12.939 ng/mL had a moderate performance as a predictor of significant liver fibrosis in chronic hepatitis B patients.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"209 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133350815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-27DOI: 10.24293/ijcpml.v29i3.2021
Anne Marrya, B. Sidharta, Lusi Oka Wardhani, J. Suparyatmo, M. Pramudianti
Liver fibrosis caused by chronic hepatitis B infection leads to significant mortality and morbidity. Monitoring and evaluation of liver fibrosis progression depend on the ability to detect the fibrosis. Liver biopsy as a gold standard for liver fibrosis is an invasive technique, while Fibroscan ® with transient elastography as a non-invasive technique has a limitation. Therefore, a biomarker is needed to detect liver fibrosis. Beta-catenin is a multifunctional protein, which has a Wnt-regulated transcription factor and resides in hepatocytes. Hepatitis B virus infection activates Wnt/beta-catenin and affects the expression of target genes for liver fibrosis. This study aimed to analyze the diagnostic performance of plasma beta-catenin levels using transient elastography as a standard reference to assess the degree of liver fibrosis in patients with chronic hepatitis B infection. This was an observational analytic study with a cross-sectional design. The analysis was performed on 70 chronic Hepatitis B patients between December 2020 and January 2021 at Dr. Moewardi Hospital, Surakarta. This ROC analysis was used to determine the cut-off point. The best AUC point was chosen using a 2x2 diagnostic test table. The cut-off point for plasma beta-catenin was 73.132 pg/mL and AUC was 0.793 (CI 95%: 0.681-0.906; p<0.001), indicating that the results were statistically significant with p<0.05. Sensitivity of 74.3%; specificity of 71.4%; PPV of 72.2%; NPV of 73.5%; LR (+) 2.6; LR (-) 0.36 were obtained. The beta-catenin level was <73.132 pg/mL. A total of 26 subjects were at risk for liver fibrosis with transient elastography >8 kPa. Plasma beta-catenin levels had moderate performance as a liver fibrosis marker.
{"title":"Beta-Catenin Plasma Test: Liver Fibrosis Degree Assessment in Chronic Hepatitis B Patients","authors":"Anne Marrya, B. Sidharta, Lusi Oka Wardhani, J. Suparyatmo, M. Pramudianti","doi":"10.24293/ijcpml.v29i3.2021","DOIUrl":"https://doi.org/10.24293/ijcpml.v29i3.2021","url":null,"abstract":"Liver fibrosis caused by chronic hepatitis B infection leads to significant mortality and morbidity. Monitoring and evaluation of liver fibrosis progression depend on the ability to detect the fibrosis. Liver biopsy as a gold standard for liver fibrosis is an invasive technique, while Fibroscan ® with transient elastography as a non-invasive technique has a limitation. Therefore, a biomarker is needed to detect liver fibrosis. Beta-catenin is a multifunctional protein, which has a Wnt-regulated transcription factor and resides in hepatocytes. Hepatitis B virus infection activates Wnt/beta-catenin and affects the expression of target genes for liver fibrosis. This study aimed to analyze the diagnostic performance of plasma beta-catenin levels using transient elastography as a standard reference to assess the degree of liver fibrosis in patients with chronic hepatitis B infection. This was an observational analytic study with a cross-sectional design. The analysis was performed on 70 chronic Hepatitis B patients between December 2020 and January 2021 at Dr. Moewardi Hospital, Surakarta. This ROC analysis was used to determine the cut-off point. The best AUC point was chosen using a 2x2 diagnostic test table. The cut-off point for plasma beta-catenin was 73.132 pg/mL and AUC was 0.793 (CI 95%: 0.681-0.906; p<0.001), indicating that the results were statistically significant with p<0.05. Sensitivity of 74.3%; specificity of 71.4%; PPV of 72.2%; NPV of 73.5%; LR (+) 2.6; LR (-) 0.36 were obtained. The beta-catenin level was <73.132 pg/mL. A total of 26 subjects were at risk for liver fibrosis with transient elastography >8 kPa. Plasma beta-catenin levels had moderate performance as a liver fibrosis marker.","PeriodicalId":354500,"journal":{"name":"Indonesian Journal of Clinical Pathology and Medical Laboratory","volume":"188 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116453194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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