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Development of a procedure involving artifact examination to determine the species affiliation of its biological material, as illustrated by an attempt to demystify an alleged Nazi lampshade made from human skin. 开发了一种涉及人工制品检查的程序,以确定其生物材料的物种归属,如试图揭开一个由人类皮肤制成的所谓纳粹灯罩的神秘面纱。
Q4 Medicine Pub Date : 2022-01-01 DOI: 10.4467/16891716AMSIK.22.024.17624
Maciej Trzciński, Anna Karpiewska, Agnieszka Hałoń, Anna Jonkisz, Arleta Lebioda, Tomasz Jurek, Tadeusz Dobosz
Objectives The main purpose of the study was to identify the species origin of the material from which the incriminating lampshade bought at a flea market had been made. Methods The histological and molecular biology methods commonly used in forensic genetics were selected to achieve this goal. The DNA for the research was isolated using a QIAamp DNA Mini Kit (Qiagen) according to the manufacturer's protocol for tissues. The quantitative and qualitative evaluation of genetic material was carried out by the real-time PCR method with a Quantifiler Duo DNA Quantification Kit (Applied Biosystems). Specific genetic markers of mtDNA of cattle, equines, deer, wild boar, and sheep were selected to identify species. Results Histological tests showed that the lampshade had been made from intestinal flaps. The DNA from sample tested positive for cattle. The test results dispelled the suspicion that the researched lampshade had been made from human skin.-hour journey. The second case is a 55-year-old male assaulted with double punches in his chest and declared dead on arrival at the hospital after 30 minutes. A medicolegal autopsy and thorough investigation, in both cases, revealed cardiac tamponade due to ventricular rupture with no underlying pathology. Conclusion The proposed testing method can be used to verify the origin of the artifacts misleadingly described as made from human skin. To our knowledge, such artifacts can be found in museums and private collections. Further-more, it has been widely believed until now that human-skin products, mainly lampshades, were mass-produced in Nazi concentration camps, mainly in Buchenwald.
目的:本研究的主要目的是确定在跳蚤市场上购买的灯罩所使用的材料的物种来源。方法:采用法医遗传学常用的组织生物学和分子生物学方法。本研究使用QIAamp DNA迷你试剂盒(Qiagen)根据制造商的组织规程分离DNA。采用Quantifiler Duo DNA定量试剂盒(Applied Biosystems)实时荧光定量PCR法对遗传物质进行定量和定性评价。选择牛、马、鹿、野猪和羊的mtDNA特异性遗传标记进行物种鉴定。结果:组织学检查显示,灯罩已由肠瓣制成。牛的DNA检测呈阳性。测试结果消除了人们对所研究的灯罩是由人体皮肤制成的怀疑。小时的旅程。第二个病例是一名55岁的男性,他的胸部遭到两拳袭击,30分钟后被送往医院时被宣布死亡。法医解剖和彻底的调查,在这两个情况下,发现心脏填塞由于心室破裂没有潜在的病理。结论:所提出的检测方法可用于验证被误导为人皮制品的来源。据我们所知,这些文物可以在博物馆和私人收藏中找到。此外,直到现在,人们还普遍认为,人类皮肤产品,主要是灯罩,是在纳粹集中营(主要是布痕瓦尔德)大量生产的。
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引用次数: 0
Filter mask as a new candidate of personal belonging used in cadaver identification - a case report. 过滤口罩作为一种新的个人归属物在尸体鉴定中的应用——一例报告。
Q4 Medicine Pub Date : 2022-01-01 DOI: 10.4467/16891716AMSIK.22.004.16233
Marcin Tomsia, Kornelia Droździok, Mariusz Kobek

The case report presents an identification process based on DNA isolated from personal belongings, including a filter mask. In May 2021, an unidentified 65-year-old male corpse was revealed by the city's outskirts road. Since it was impossible to use material from living relatives for comparative analysis, the samples of personal belongings of the alleged victim were used instead: clippings of the filtering face piece type 2 (FFP2) face mask (parts adhering to the nose and the earlobes, the central part of the mask), swabs from the razor (blade and shaft), toothbrush shaft, and toothbrush filaments clippings. The presented case indicates the need for collecting a wide range of samples for genetic analyses, including filter masks as an alternative item of personal belonging.

该病例报告介绍了一种基于从个人物品(包括过滤口罩)中分离的DNA的识别过程。2021年5月,一具身份不明的65岁男性尸体在该市郊区的道路上被发现。由于不可能使用在世亲属的材料进行比较分析,因此使用了所称受害者的个人物品样本:过滤面罩2型(FFP2)的剪报(附着在鼻子和耳坠上的部分,面罩的中心部分)、剃刀(刀片和轴)的拭子、牙刷轴和牙刷丝的剪报。所提出的案例表明,需要收集广泛的样本进行遗传分析,包括过滤口罩作为个人财产的替代项目。
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引用次数: 0
[Medicolegal opinions in France in XVII Century]. [17世纪法国的法医学观点]。
Q4 Medicine Pub Date : 2022-01-01 DOI: 10.4467/16891716AMSIK.22.003.16232
Malwina K Dębicka

This article is a synthesis of the history of medicolegal opinions in France in the 17th century. Mainly two research methods were used in the work - the historical method and the descriptive method. The article aims to present the knowledge of French forensic medicine in a historical and legal context. It shows the pioneering research of medics and surgeons, presents the legal regulations of that time, and emphasizes the high level of French science compared to other European countries. It is worth adding that from the earliest times the issues with the assessment of health and life were assigned to medical experts - jurors (the edict of 1603 médecins jurés et chirurgiens jurés). During this period, forensic and medical examinations became a duty. People became interested in the mental health of the accused, as well as in the inspection of the reproductive organs in the event of a divorce. Italian surgery had a huge impact on the history of medicolegal opinions in the French territories. Interestingly, France was the first country to separate judicial and medical issues from tasks related to the medical police.

这篇文章综合了17世纪法国的法医学观点史。本研究主要采用了两种研究方法:历史研究法和描述研究法。本文旨在介绍法国法医学在历史和法律背景下的知识。它展示了医生和外科医生的开创性研究,展示了当时的法律法规,并强调了法国与欧洲其他国家相比的高科学水平。值得补充的是,从最早的时候起,健康和生命评估的问题就被指派给医学专家——陪审员(1603年《msamudecins juret chirurgiens jur》法令)。在此期间,法医和医疗检查成为一项职责。人们开始关心被告的心理健康,以及在离婚情况下对生殖器官的检查。意大利外科手术对法国领土上的医法学观点产生了巨大的影响。有趣的是,法国是第一个将司法和医疗问题与与医务警察有关的任务分开的国家。
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引用次数: 0
Simultaneous poisoning of 48 birds of prey - bendiocarb determination with the use of UHPLC-ESI-MS/MS method in fatal case from Eastern Europe. 东欧48只猛禽同时中毒——高效液相色谱-高效液相色谱-质谱联用法测定杀虫威
Q4 Medicine Pub Date : 2022-01-01 DOI: 10.4467/16891716AMSIK.22.009.16807
Agnieszka Chłopaś-Konowałek, Marcin Zawadzki, Łukasz Kurach, Olga Wachełko, Rafał Ciaputa, Kaja Tusiewicz, Paweł Szpot

Aim: Bendiocarb is used against a wide range of insects but has already been withdrawn from the market in some countries. It poses a high risk to birds as they can accidentally ingest it while searching for food, followed by toxic effects. This paper presents the results of toxicological and histopathological studies of 48 cases of intentional birds of prey poisoning with bendiocarb in Eastern Europe, specifically Poland.

Material and methods: A novel ultra-high-performance liquid chromatography-triple quadrupole-tandem mass spectrometry (UHPLC-ESI-MS/MS) method for bendiocarb determination in animal liver samples was developed and fully validated. The sample preparation technique was based on one-step precipitation of proteins with cold acetonitrile. The internal standard used was carbaryl-d7. Full time of analysis was less than 10 minutes. The application of the UHPLC-ESI-MS/MS method allowed us to achieve the lowest LOQ (1 ng/g) of bendiocarb in biological samples to date.

Results: Necropsies and histopathological examinations of common ravens (Corvus corax), western marsh harriers (Circus aeruginosus), red kites (Milvus milvus), and a white-tailed eagle (Haliaeetus albicilla) revealed multi-organ toxicity manifested as congestion, oedema, or stagnation of blood. An analytical investigation confirmed the presence of bendiocarb in liver in the 1808-7721 ng/g range. Furthermore, the presence of this compound was qualitatively confirmed in the stomach and beak contents and also in the bait located near the deceased animals.

Conclusions: A comprehensive forensic examination is crucial to monitor wildlife fatalities, especially applying a combined analytical and histopathological approach to identify and eliminate highly toxic substances which pose a threat to the ecosystem.

目的:恶虫威用于对付多种昆虫,但在一些国家已经从市场上撤出。它对鸟类构成了很高的风险,因为它们在寻找食物时可能不小心摄入了它,然后产生毒性作用。本文介绍了在东欧,特别是波兰的48例故意用苯虫威毒死猛禽的毒理学和组织病理学研究的结果。材料与方法:建立了一种超高效液相色谱-三重四极杆串联质谱(UHPLC-ESI-MS/MS)测定动物肝脏样品中苯并威的新方法,并进行了充分验证。样品制备技术基于冷乙腈一步沉淀蛋白质。所使用的内标为卡巴瑞尔- 7。整个分析时间不到10分钟。应用UHPLC-ESI-MS/MS方法,我们在生物样品中获得了迄今为止最低的LOQ (1 ng/g)。结果:对普通乌鸦(Corvus corax)、西部沼泽鹞(Circus aeruginosus)、红鸢(Milvus Milvus)和白尾鹰(Haliaeetus albicilla)的尸检和组织病理学检查显示多器官毒性,表现为充血、水肿或瘀血。一项分析调查证实,在1808-7721纳克/克范围内,肝脏中存在恶虫威。此外,该化合物的存在定性地证实了胃和喙内容物以及位于死亡动物附近的诱饵的存在。结论:全面的法医检查对于监测野生动物死亡至关重要,特别是应用分析和组织病理学相结合的方法来识别和消除对生态系统构成威胁的高毒性物质。
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引用次数: 0
[Analysis of suicide cases in the autopsy material of the Department of Forensic Medicine Collegium Medicum in Bydgoszcz, University of Nicolaus Copernicus in Toruń in 2011-2020]. [2011-2020年托洛茨基哥白尼大学比得哥什法医学院法医系尸检材料中自杀病例分析]。
Q4 Medicine Pub Date : 2022-01-01 DOI: 10.4467/16891716AMSIK.22.010.16808
Anna Dropiewska-Nowak, Magdalena Cychowska

Aim: Analysis of suicides on the basis of forensic post mortem examinations conducted at the Department of Forensic Medicine in Bydgoszcz in comparison with data from previous years and other centers.

Material and methods: The assessment included 6,564 forensic medical opinions from the post mortem examinations carried out in 2011-2020, including 1,073 cases of suicide, taking into account the sex and age of the victims, the place where the suicide was committed, the influence of alcohol or other intoxicants, methods of committing suicides and date (month and year).

Results: Suicides accounted for 16.34% of all post mortem examinations. The most common method of suicide was hanging - 73%. The second most common way to take your own life was fall from height - 8%. The most numerous age group among those committing suicide are people between 51 and 60 years of age. In most cases, the suicide victims were sober. There is a significant difference between the state of sobriety and the gender of the victim. Male victims were much more often under the influence of alcohol during the suicide. 14% of all suicides were committed by women, and 86% by men. Death as a result of suicide took place somewhat more often within the boundaries of large urban agglomerations than in towns and villages.

Conclusions: The research showed an increase in the suicide rate compared to the previous years. Hanging is still the most common form of suicide. There has been an increase in the number of suicides in the elderly and an increase in the number of so-called combined suicides.

目的:根据比得哥什市法医学部门法医尸检的数据,与往年及其他中心的数据进行比较分析自杀事件。材料和方法:评估包括从2011-2020年进行的尸检中得出的6 564份法医意见,其中包括1 073起自杀案件,同时考虑到受害者的性别和年龄、自杀地点、酒精或其他麻醉品的影响、自杀方法和日期(月份和年份)。结果:自杀占所有尸检的16.34%。最常见的自杀方式是上吊,占73%。第二常见的自杀方式是从高处坠落,占8%。自杀人数最多的年龄组是51岁至60岁的人。在大多数情况下,自杀者都是清醒的。在清醒状态和受害者的性别之间存在显著差异。男性受害者在自杀时往往受到酒精的影响。14%的自杀者是女性,86%是男性。自杀造成的死亡在大城市的边界内比在城镇和村庄更常发生。结论:研究表明,与前几年相比,自杀率有所上升。上吊仍然是最常见的自杀方式。老年人自杀的人数有所增加,所谓的联合自杀人数也有所增加。
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引用次数: 0
Recovery techniques for contact DNA traces. 接触DNA痕迹恢复技术。
Q4 Medicine Pub Date : 2022-01-01 DOI: 10.4467/16891716AMSIK.22.016.17394
Agnieszka Gołaszewska

Donor DNA profiling can serve at least two purposes: 1) to enhance the evidential value of DNA deposited on garments/ items and 2) to provide valuable tactical information during crime scene investigation. In this review, different types of methods for the recovery of the contact DNA traces have been summarized. Additionally, with the available techniques, the unique characteristics and limitations thereof have been overviewed. The aim of this paper is to review the techniques of touch traces collection.

供体DNA分析至少有两个目的:1)提高存放在服装/物品上的DNA的证据价值;2)在犯罪现场调查期间提供有价值的战术信息。本文综述了几种恢复接触DNA痕迹的方法。此外,利用可用的技术,概述了其独特的特征和局限性。本文的目的是回顾触摸痕迹收集技术。
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引用次数: 0
[The use of clinical molecular and genetic tests in forensic medical opinions]. [在法医意见中使用临床分子和基因测试]。
Q4 Medicine Pub Date : 2022-01-01 DOI: 10.4467/16891716AMSIK.22.005.16234
Rafał Skowronek

Modern molecular biology and clinical genetics have the ability to diagnose many diseases, the presence or absence of which is important in various areas of forensic medical opinion. The aim of the study is to present examples of possible non-standard application of clinical molecular-genetic tests in forensic medicine. From among all the opinions prepared so far at the Department of Forensic Medicine in Katowice, three were selected two of which concerned post-autopsy conclusions relating to the cause of death, and in one case - opinion based on the files of a case related to the suspicion of a maltreated child syndrome in an infant. In all these cases, the results of the molecular and genetic tests were negative, which was taken into account by the experts when formulating the final conclusions of the opinion. The use of clinical molecular and genetic tests in forensic medicine is possible and always brings important additional information that broadens the possibilities of opinion-making and facilitates diagnosis. The areas in which this type of research may be particularly useful are: diagnostics of the basis of thromboembolic complications, modern integrated morphological and molecular diagnostics of neoplasms and diagnostics of genetic defects of connective tissue in children suspected of having abused child syndrome.

现代分子生物学和临床遗传学有能力诊断许多疾病,这些疾病的存在与否在法医意见的各个领域都很重要。该研究的目的是提出在法医学中可能的非标准应用临床分子基因测试的例子。在卡托维兹法医司迄今编写的所有意见中,选出了三份意见,其中两份涉及与死因有关的尸检后结论,还有一份意见是根据与怀疑一名婴儿患有受虐待儿童综合症有关的案件档案提出的意见。在所有这些案件中,分子和基因检测的结果都是阴性的,专家们在制定意见的最后结论时考虑到了这一点。在法医中使用临床分子和基因测试是可能的,并且总是带来重要的额外信息,从而扩大了发表意见的可能性并促进了诊断。这类研究可能特别有用的领域是:血栓栓塞并发症的基础诊断,肿瘤的现代综合形态学和分子诊断,以及怀疑患有虐待儿童综合症的儿童结缔组织遗传缺陷的诊断。
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引用次数: 0
Restraint asphyxia. An analysis of the circumstances and mechanism of death in agitated, physically restrained individuals. 克制窒息。对激动、身体受约束的个体的死亡情况和机制的分析。
Q4 Medicine Pub Date : 2022-01-01 DOI: 10.4467/16891716AMSIK.22.002.16231
Tomasz Konopka, Krzysztof Woźniak, Artur Moskała, Paweł Kopacz, Marcin Strona, Ewa Rzepecka-Woźniak, Piotr Kluza, Ewa Juźwik-Kopacz, Filip Bolechała

Recent years saw frequent media reports of young people who die while they are being arrested by the police. Death in these circumstances affects people who are agitated and restrained with the use of force, with their autopsies indicating no unequivocally traumatic cause of death. The goal of this study was to identify the mechanism and circumstances of sudden deaths in agitated individuals who are being restrained. Ten cases evaluated at our center since 2010 were included in this study: nine involved forensic postmortem examination and one involved casefile analysis. In each case there was sudden cardiac arrest or at least a loss of consciousness, and the cardiopulmonary resuscitation proved ineffective. In six cases the cause of agitation was acute psychosis, in four it was an effect of narcotics, usually in high concentration. Conjunctival petechiae were detected in only five cases. The most probable cause of death in the evaluated cases was the combination of physical exertion caused by pathological psychomotor agitation and forcible restraint, hindering the function of the respiratory system. This mechanism is known as restraint asphyxia.

近年来,媒体经常报道年轻人在被警察逮捕时死亡。这些情况下的死亡影响的是被激怒和被武力约束的人,他们的尸检没有明确的创伤性死亡原因。本研究的目的是确定被约束的激动个体猝死的机制和情况。本研究纳入本中心自2010年以来评估的10例病例,其中9例涉及法医尸检,1例涉及案件档案分析。在每个病例中,都出现了心脏骤停或至少失去意识,心肺复苏被证明无效。在六个病例中,躁动的原因是急性精神病,在四个病例中,这是麻醉剂的作用,通常是高浓度的。结膜积点仅5例。在评估的病例中,最可能的死亡原因是病理性精神运动性激动引起的体力消耗和强制约束的结合,阻碍了呼吸系统的功能。这种机制被称为抑制性窒息。
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引用次数: 0
Verification of insertion-deletion markers (InDels) and microsatellites (STRs) as subsidiary tools for inferring Slavic population ancestry. 插入-删除标记(InDels)和微卫星(STRs)作为推断斯拉夫人群祖先的辅助工具的验证。
Q4 Medicine Pub Date : 2022-01-01 DOI: 10.4467/16891716AMSIK.22.015.17393
Karolina Hołub, Boris A Malyarchuk, Miroslava V Derenko, Nataša Kovačević-Grujičić, Milena Stevanović, Danijela Drakulić, Slobodan Davidović, Tomasz Grzybowski

Genetic markers for the prediction of biogeographical ancestry have proved to be effective tools for law enforcement agencies for many years now. In this study, we attempted to assess the potential of insertion-deletion markers (InDel) and microsatellites (STRs) as subsidiary polymorphisms for inference of Slavic population ancestry. For that purpose, we genotyped Slavic-speaking populations samples from Belarus, the Czech Republic, Poland, Serbia, Ukraine and Russia in 46 InDels and 15 STRs by PCR and capillary electrophoresis and analyzed for between-population differentiation with the use of distance-based methods (FST, principal component analysis and multidimensional scaling). Additionally, we studied a sample from a Polish individual of well-documented genealogy whose biogeographic ancestry had previously been inferred by commercial genomic services using autosomal single nucleotide polymorphisms (SNPs), mitochondrial DNA and Y-SNP markers. For comparative purposes, we used genotype data collected in the "forInDel" browser and allele frequencies from previously published papers. The results obtained for InDels and STRs show that the Slavic populations constitute a genetically homogeneous group, with the exception of the Czechs differing clearly from the other tested populations. The analysis of the known Polish sample in the Snipper application proves the usefulness of the InDel markers on the continental level only. Conversely, microsatellites not only improve prediction, but are also informative if considered as an independent set of ancestry markers.

多年来,用于预测生物地理祖先的遗传标记已被证明是执法机构的有效工具。在这项研究中,我们试图评估插入-删除标记(InDel)和微卫星(STRs)作为推断斯拉夫人群祖先的辅助多态性的潜力。为此,我们通过PCR和毛细管电泳对来自白俄罗斯、捷克、波兰、塞尔维亚、乌克兰和俄罗斯的46个InDels和15个STRs的斯拉夫语人群样本进行了基因分型,并使用基于距离的方法(FST、主成分分析和多维标度)分析了群体间的差异。此外,我们还研究了一个波兰个体的样本,该个体的家谱记录良好,其生物地理祖先此前已通过商业基因组服务使用常染色体单核苷酸多态性(snp)、线粒体DNA和Y-SNP标记推断出来。为了进行比较,我们使用了在“forInDel”浏览器中收集的基因型数据和先前发表的论文中的等位基因频率。从InDels和STRs中获得的结果表明,除了捷克人与其他测试人群明显不同外,斯拉夫人口构成了一个基因同质的群体。对Snipper应用程序中已知的波兰样品的分析证明了InDel标记仅在大陆水平上的有用性。相反,微卫星不仅可以提高预测,而且如果作为一组独立的祖先标记,还可以提供信息。
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引用次数: 0
Medico-legal analysis of cases of children who died suddenly due to pneumonia undiagnosed in their lifetime. 儿童一生中未确诊肺炎猝死病例的法医学分析。
Q4 Medicine Pub Date : 2022-01-01 DOI: 10.4467/16891716AMSIK.22.020.17620
Michał Szczepański, Rafał Skowronek, Beata Sarecka-Hujar, Ilona Kopyta

Pneumonia is one of the most common causes of children's hospitalization and death. The aim of the study was a medico-legal analysis of children who died suddenly due to pneumonia undiagnosed in their lifetime. The research was of a retrospective character and consisted in an analysis of prosecution files. The study included 47 children who died between 2011-2018 in whom pneumonia after post-mortem examination was indicated as the cause of death, as well as children in whom the cause of death, despite additional post-mortem examinations, including histopathological tests, was not established. In some cases, under additional post-mortem examinations, additional targeted immunohistochemical staining of selected lung sections was performed to establish the diagnosis. In children with prodromal symptoms, histopathological examination showed significantly more frequent atelectasis than in children without prodromal symptoms. Pneumonia is a significant clinical problem. Especially in young children, it may proceed with- out any symptoms that would cause such a diagnosis to be made. A properly conducted post-mortem diagnosis supplemented by immunohistochemical examinations allows to reduce the number of unexplained deaths in children.

肺炎是儿童住院和死亡的最常见原因之一。这项研究的目的是对一生中因未确诊的肺炎而突然死亡的儿童进行医学-法律分析。这项研究是回顾性的,包括对起诉档案的分析。该研究包括2011-2018年期间死亡的47名儿童,其中尸检后的肺炎被认为是死亡原因,以及尽管进行了包括组织病理学检查在内的其他尸检检查,但死因仍未确定的儿童。在一些病例中,在额外的死后检查下,对选定的肺切片进行额外的靶向免疫组织化学染色以确定诊断。在有前驱症状的儿童中,组织病理学检查显示肺不张明显多于无前驱症状的儿童。肺炎是一个重要的临床问题。特别是在年幼的儿童中,它可能在没有任何症状的情况下进行,而这些症状会导致做出这样的诊断。适当的尸检诊断加上免疫组织化学检查可以减少儿童不明原因死亡的数量。
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引用次数: 0
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