{"title":"A Study of Prevalence of Co-Morbidities, Self- Management and Awareness in Type II Multifactorial Diabetes Mellitus Patients: A Cross Sectional Study Conducted in The Darjeeling Hills Region of West Bengal, India","authors":"Yuvraj Gurung, Karun Chhetri","doi":"10.53555/jaz.v44i5.2898","DOIUrl":"https://doi.org/10.53555/jaz.v44i5.2898","url":null,"abstract":"","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139164595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The proposed HPLC Method was found to be simple,precise,accurate and economicalfor the estimation of dutasteride in Bulk and tablet dosage form. The present work, developed HPLC method was found suitable and validated. Luna – C-18 (250 mm x 4.6 mm, 20 µL) column was used for the assay. Mobile phase was Acetonitrile and Water in the ratio 80:20. Linearity was established in the range of 5-100 µg/ml with a coefficient correlation of 0.9998 and eluents were monitored at 242 nm and at 1 ml/min. flow rate. The Retention time was found to be 5.9 min. The efficiency of the column, expressed as the number of theoretical plates for six replicate injections was 11566 ± 119.695 (%CV1.03%) and USP tailing factor was 1.25 ± 0.012 (%CV 0.79). The regression equation for the calibration plot was Y = 0.0618 X – 0.008. Intra-day precision from 0.166 to 1.449 and Inter-day precision from 0.186 to 1.449. The% recovery of dutasteride from API and dosage form were 100.70% and 99.66%. The drug was subjected to stress conditions such as Oxidative and Photodegradation, Considerable degradation was found to occure in acidic and basic medium on heating at 100°C but no degradation was observed at room temperature. The drug was exposed to 10% H2O2{heating at 100°C forming degradation product at RT: 2.557}. The LOD and LOQ was 0.257µg and 0.779 µg respectively.When the flow rate was reduced, the retention time of dutasteride increased.The assay result with analyst #1 and Analyst #2 were % Assay = 98.40% (%RSD = 0.13%) and % Assay = 99.07% (%RSD =0.53) respectively. Robustness and ruggednesswasobserved that results were well within acceptance limits of 98–102%, with %RSD ±2.0%, indicating the method is rugged and provides consistent and reliable results which are not affected by small changes in experimental conditions. This method can be used for routine analysis of dutasteride. Running Title: A New Method Development and Validation of 5α-Reductase inhibitor using R-HPLC.
{"title":"A New Method Development and Validation of 5α Reductase Inhibitor (Dutasteride) Using R-HPLC In Bulk and Pharmaceutical Dosage Form (Dexamethasone Use as An Internal Standard)","authors":"Mahtab Ali, D. K. Sharma","doi":"10.17762/jaz.v44i5.2772","DOIUrl":"https://doi.org/10.17762/jaz.v44i5.2772","url":null,"abstract":"The proposed HPLC Method was found to be simple,precise,accurate and economicalfor the estimation of dutasteride in Bulk and tablet dosage form. The present work, developed HPLC method was found suitable and validated. Luna – C-18 (250 mm x 4.6 mm, 20 µL) column was used for the assay. Mobile phase was Acetonitrile and Water in the ratio 80:20. Linearity was established in the range of 5-100 µg/ml with a coefficient correlation of 0.9998 and eluents were monitored at 242 nm and at 1 ml/min. flow rate. The Retention time was found to be 5.9 min. The efficiency of the column, expressed as the number of theoretical plates for six replicate injections was 11566 ± 119.695 (%CV1.03%) and USP tailing factor was 1.25 ± 0.012 (%CV 0.79). The regression equation for the calibration plot was Y = 0.0618 X – 0.008. Intra-day precision from 0.166 to 1.449 and Inter-day precision from 0.186 to 1.449. The% recovery of dutasteride from API and dosage form were 100.70% and 99.66%. The drug was subjected to stress conditions such as Oxidative and Photodegradation, Considerable degradation was found to occure in acidic and basic medium on heating at 100°C but no degradation was observed at room temperature. The drug was exposed to 10% H2O2{heating at 100°C forming degradation product at RT: 2.557}. The LOD and LOQ was 0.257µg and 0.779 µg respectively.When the flow rate was reduced, the retention time of dutasteride increased.The assay result with analyst #1 and Analyst #2 were % Assay = 98.40% (%RSD = 0.13%) and % Assay = 99.07% (%RSD =0.53) respectively. Robustness and ruggednesswasobserved that results were well within acceptance limits of 98–102%, with %RSD ±2.0%, indicating the method is rugged and provides consistent and reliable results which are not affected by small changes in experimental conditions. This method can be used for routine analysis of dutasteride. Running Title: A New Method Development and Validation of 5α-Reductase inhibitor using R-HPLC.","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138973217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-14DOI: 10.17762/jaz.v44is7.2721
Fatima M Inamdar, Sateesh Ambesange, Renuka Mane, Hasan Hussain, Sahil Wagh, Prachi Lakhe
This paper represents a thorough method for integrating emotions, texttospeech conversion, and state of the art voice cloning. The paper focuses on novel background noise adaptation, emotional voice synthesis, and multi-speaker voice cloning for better speech synthesis. The synthesis of emotive voices, multi-speaker voice cloning, and creative methods for modifying background noise to improve speech synthesis quality are among the topics covered in this study. Additionally, the study explores the domain of emotional artificial intelligence by adding a variety of emotions to artificial voices, improving user engagement through sympathetic reactions. The study also looks at how background noise can be altered to change it from a disturbing to a silent, non-disruptive state. The texttospeech systems usability in noisy conditions is greatly enhanced by this improvement. By integrating these components, the project makes a substantial contribution to text to speech, emotional AI, and voice cloning, creating new avenues for human-computer connection.
{"title":"Voice Cloning Using Artificial Intelligence and Machine Learning: A Review","authors":"Fatima M Inamdar, Sateesh Ambesange, Renuka Mane, Hasan Hussain, Sahil Wagh, Prachi Lakhe","doi":"10.17762/jaz.v44is7.2721","DOIUrl":"https://doi.org/10.17762/jaz.v44is7.2721","url":null,"abstract":"This paper represents a thorough method for integrating emotions, texttospeech conversion, and state of the art voice cloning. The paper focuses on novel background noise adaptation, emotional voice synthesis, and multi-speaker voice cloning for better speech synthesis. The synthesis of emotive voices, multi-speaker voice cloning, and creative methods for modifying background noise to improve speech synthesis quality are among the topics covered in this study. Additionally, the study explores the domain of emotional artificial intelligence by adding a variety of emotions to artificial voices, improving user engagement through sympathetic reactions. The study also looks at how background noise can be altered to change it from a disturbing to a silent, non-disruptive state. The texttospeech systems usability in noisy conditions is greatly enhanced by this improvement. By integrating these components, the project makes a substantial contribution to text to speech, emotional AI, and voice cloning, creating new avenues for human-computer connection.","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138974159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food waste utilisation is the very current global concern at today’s world. Fruits and vegetables are the foremost part of balanced diet. The cycle of healthy diet is not fulfilled without fruits. Furthermore, fruit peels possess also great benefits for people health. Researcher are trying to make new food products from different food wastes. From recent research study it was found that pomegranate peels, mango peels, orange peels, banana peels which contribute enormous good efficacy to provide good health status. These waste constituent help to develop different new fortified food products, which contain lots of bioactive compounds that helps to mitigate acute to chronic ailments. This review article will be discussed an up-to-date information about uses of food wastes for manufacturing new fortified food products.
{"title":"A Review on Food Wastes for Manufacturing New Food Products","authors":"Samapti Bedi, 2Souvik Tewari, Bidyut Bandhopadhyay, Prathiksa Pramanik","doi":"10.17762/jaz.v44is6.2797","DOIUrl":"https://doi.org/10.17762/jaz.v44is6.2797","url":null,"abstract":"Food waste utilisation is the very current global concern at today’s world. Fruits and vegetables are the foremost part of balanced diet. The cycle of healthy diet is not fulfilled without fruits. Furthermore, fruit peels possess also great benefits for people health. Researcher are trying to make new food products from different food wastes. From recent research study it was found that pomegranate peels, mango peels, orange peels, banana peels which contribute enormous good efficacy to provide good health status. These waste constituent help to develop different new fortified food products, which contain lots of bioactive compounds that helps to mitigate acute to chronic ailments. This review article will be discussed an up-to-date information about uses of food wastes for manufacturing new fortified food products.","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138974670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-14DOI: 10.17762/jaz.v44is7.2808
Catherine Moanis Labib, Prof Dr. Zakaria Mowafy Emam Mowafy, Prof. Dr. Ayman Abdel Samea Gaber, Assist Prof. Dr. Khadra Mohamed Ali
Objective: to evaluate the efficacy of GA-AS laser against microcurrent electrical stimulation on postmastectomy shoulder pain as well as lymphedema. Method: a double blinded randomized controlled study. sixty breast cancer patients with lymph-edema stage I and II ,40-55 years old were assigned randomly into study -group A, n = 20 or study -group B, (n = 20) in addition to control group C, (n = 20). The study group was given 20 minutes GA-AS laser, 90 minutes complete decongestive therapy (CDT). study -group B received 20 minutes of microcurrent electrical stimulation (MENS) and 90minutes CDT, control group C was given 90 minutes CDT. All treatment interventions were applied at a frequency of three sessions per week for 12 weeks. Arm volume calculated by circumference measurement, Shoulder and Hand (DASH) questionnaire as well as pain intensity utilizing a visual analogue scale (VAS) were measured at before and following 12 weeks of treatment. Results: a statistically significant difference has been detected in limb volume, DASH questionnaire as well as VAS favoring the study group A ((p = 0.001).) and the study group B ((p = 0.001). following 12 weeks of intervention. The mean (SD) for limb-volume, DASH questionnaire as well as pain score were 1236.74 ± 43.34, 27.21 ± 7.16, and 3.20 ± 0.62in the study group A, and 1331.76 ± 46.92, 34.52 ± 4.38 and 3.90 ± 0.78in the study group B and 1390.18 ± 58.77 ml, 40.68 ± 6.93and 5.30 ± 0.86in the control group C respectively. Conclusion: adding GA-AS laser and microcurrent electrical stimulation to the conventional physical therapy program had a valuable effect than traditional physical therapy program alone in improving postmastectomy shoulder pain as well as lymphedema.
目的：评估 GA-AS 激光与微电流电刺激对乳腺癌切除术后肩部疼痛和淋巴水肿的疗效。方法：双盲随机对照研究。60 名淋巴水肿 I 期和 II 期乳腺癌患者（40-55 岁）被随机分配到研究 A 组（20 人）或研究 B 组（20 人）以及对照 C 组（20 人）。研究 A 组接受 20 分钟 GA-AS 激光治疗和 90 分钟完全缓解充血疗法（CDT），研究 B 组接受 20 分钟微电流电刺激（MENS）和 90 分钟 CDT，对照 C 组接受 90 分钟 CDT。所有治疗干预均以每周三次的频率进行，为期 12 周。在治疗前和治疗 12 周后，通过周长测量、肩部和手部（DASH）问卷以及视觉模拟量表（VAS）测量疼痛强度来计算手臂的体积。结果：在干预 12 周后，研究 A 组（（P = 0.001））和研究 B 组（（P = 0.001））在肢体体积、DASH 问卷和 VAS 方面存在明显差异。研究 A 组的肢体体积、DASH 问卷和疼痛评分的平均值（标清）分别为（1236.74 ± 43.34）、（27.21 ± 7.16）和（3.20 ± 0.62）；研究 B 组的肢体体积、DASH 问卷和疼痛评分的平均值（标清）分别为（1331.76 ± 46.92）、（34.52 ± 4.38）和（3.90 ± 0.78）；对照 C 组的肢体体积、DASH 问卷和疼痛评分的平均值（标清）分别为（1390.18 ± 58.77）毫升、（40.68 ± 6.93）毫升和（5.30 ± 0.86）毫升。结论：在传统物理治疗方案的基础上增加 GA-AS 激光和微电流电刺激，在改善乳房切除术后肩部疼痛和淋巴水肿方面比单独使用传统物理治疗方案更有价值。
{"title":"Post-Mastectomy Shoulder Pain and Lymphedema Responses to Ga-As Laser Versus Microcurrent Electrical Stimulation","authors":"Catherine Moanis Labib, Prof Dr. Zakaria Mowafy Emam Mowafy, Prof. Dr. Ayman Abdel Samea Gaber, Assist Prof. Dr. Khadra Mohamed Ali","doi":"10.17762/jaz.v44is7.2808","DOIUrl":"https://doi.org/10.17762/jaz.v44is7.2808","url":null,"abstract":"Objective: to evaluate the efficacy of GA-AS laser against microcurrent electrical stimulation on postmastectomy shoulder pain as well as lymphedema. Method: a double blinded randomized controlled study. sixty breast cancer patients with lymph-edema stage I and II ,40-55 years old were assigned randomly into study -group A, n = 20 or study -group B, (n = 20) in addition to control group C, (n = 20). The study group was given 20 minutes GA-AS laser, 90 minutes complete decongestive therapy (CDT). study -group B received 20 minutes of microcurrent electrical stimulation (MENS) and 90minutes CDT, control group C was given 90 minutes CDT. All treatment interventions were applied at a frequency of three sessions per week for 12 weeks. Arm volume calculated by circumference measurement, Shoulder and Hand (DASH) questionnaire as well as pain intensity utilizing a visual analogue scale (VAS) were measured at before and following 12 weeks of treatment. Results: a statistically significant difference has been detected in limb volume, DASH questionnaire as well as VAS favoring the study group A ((p = 0.001).) and the study group B ((p = 0.001). following 12 weeks of intervention. The mean (SD) for limb-volume, DASH questionnaire as well as pain score were 1236.74 ± 43.34, 27.21 ± 7.16, and 3.20 ± 0.62in the study group A, and 1331.76 ± 46.92, 34.52 ± 4.38 and 3.90 ± 0.78in the study group B and 1390.18 ± 58.77 ml, 40.68 ± 6.93and 5.30 ± 0.86in the control group C respectively. Conclusion: adding GA-AS laser and microcurrent electrical stimulation to the conventional physical therapy program had a valuable effect than traditional physical therapy program alone in improving postmastectomy shoulder pain as well as lymphedema.","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139001656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Hemiparesis is a debilitating condition characterized by weakened muscle strength in one side of the body, often caused by stroke or brain injury. Rehabilitation is crucial for improving upper limb muscle strength and functional recovery in individuals with hemiparesis. Mobile-based Virtual Reality (VR) programs have emerged as an innovative and cost-effective approach to augment conventional physiotherapy in rehabilitation. This study investigates the efficacy of a low-cost mobile VR intervention in enhancing upper limb muscle strength and motivating individuals with hemiparesis to actively engage in their recovery. Methods: A quasi-experimental study was conducted with 20 participants, divided into two groups: an experimental group receiving the combined intervention of mobile-based VR programs and conventional physiotherapy, and a control group receiving only conventional physiotherapy. The intervention spanned four weeks, with sessions held three days a week. Pre-test and post-test measurements were taken for various upper limb muscle groups using Manual Muscle Testing (MMT). Correlation analyses were conducted to assess the effectiveness of the intervention. Results: The results revealed a strong and statistically significant positive correlation between pre-test and post-test MMT scores in the experimental group, indicating substantial improvements in upper limb muscle strength. In contrast, the control group exhibited more modest gains. The integration of mobile-based VR programs led to increased motivation and engagement among participants, contributing to better adherence to the rehabilitation routine. Conclusion: The integration of affordable mobile VR solutions in hemiparesis rehabilitation presents a promising approach to empower individuals on their journey to recovery. By improving upper limb muscle strength and motivating active participation in rehabilitation, mobile VR interventions like "CyberRehab" have the potential to revolutionize the field of hemiparesis recovery, ultimately enhancing the quality of life for those affected by this condition.
{"title":"Cyberrehab: Empowering Hemiparesis Recovery with Affordable Mobile Virtual Reality Solutions for Upper Limb Strength","authors":"Anchit Gugnani, Nandini Kushwaha, Danishta Danishta, Richa Uniyal, Niketa Shobhit, Neha Vyas","doi":"10.17762/jaz.v44is7.2739","DOIUrl":"https://doi.org/10.17762/jaz.v44is7.2739","url":null,"abstract":"Background: Hemiparesis is a debilitating condition characterized by weakened muscle strength in one side of the body, often caused by stroke or brain injury. Rehabilitation is crucial for improving upper limb muscle strength and functional recovery in individuals with hemiparesis. Mobile-based Virtual Reality (VR) programs have emerged as an innovative and cost-effective approach to augment conventional physiotherapy in rehabilitation. This study investigates the efficacy of a low-cost mobile VR intervention in enhancing upper limb muscle strength and motivating individuals with hemiparesis to actively engage in their recovery. Methods: A quasi-experimental study was conducted with 20 participants, divided into two groups: an experimental group receiving the combined intervention of mobile-based VR programs and conventional physiotherapy, and a control group receiving only conventional physiotherapy. The intervention spanned four weeks, with sessions held three days a week. Pre-test and post-test measurements were taken for various upper limb muscle groups using Manual Muscle Testing (MMT). Correlation analyses were conducted to assess the effectiveness of the intervention. Results: The results revealed a strong and statistically significant positive correlation between pre-test and post-test MMT scores in the experimental group, indicating substantial improvements in upper limb muscle strength. In contrast, the control group exhibited more modest gains. The integration of mobile-based VR programs led to increased motivation and engagement among participants, contributing to better adherence to the rehabilitation routine. Conclusion: The integration of affordable mobile VR solutions in hemiparesis rehabilitation presents a promising approach to empower individuals on their journey to recovery. By improving upper limb muscle strength and motivating active participation in rehabilitation, mobile VR interventions like \"CyberRehab\" have the potential to revolutionize the field of hemiparesis recovery, ultimately enhancing the quality of life for those affected by this condition.","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139002198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-14DOI: 10.17762/jaz.v44is7.2735
Gunjan Hasijani
Purpose: The purpose of this study is to find the impact of Perceived Supervisor Support on Stress. Design/Methodology/Approach: This study is based on descriptive cum causal research design and a sample of 200 employees was randomly selected from various Aviation employees working at the ground level. Originality/value: The study examined the impact of Supervisor’s Support on Employee Stress.
{"title":"A Study on impact of Perceived Supervisor Support on Employee Stress in Aviation Sector","authors":"Gunjan Hasijani","doi":"10.17762/jaz.v44is7.2735","DOIUrl":"https://doi.org/10.17762/jaz.v44is7.2735","url":null,"abstract":"Purpose: The purpose of this study is to find the impact of Perceived Supervisor Support on Stress. \u0000Design/Methodology/Approach: This study is based on descriptive cum causal research design and a sample of 200 employees was randomly selected from various Aviation employees working at the ground level. \u0000Originality/value: The study examined the impact of Supervisor’s Support on Employee Stress.","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139002648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-14DOI: 10.17762/jaz.v44is1.2801
Elyor Imomov Abdikarimovich
This thesis explores the multifaceted nature of linguodidactics, addressing issues related to multilingualism, technology integration, language assessment, teacher training, language policies, inclusivity, motivation, and emerging trends. By examining these issues, this research aims to contribute to the enhancement of language teaching and learning practices, ultimately fostering effective communication and intercultural understanding in an increasingly globalized world.
{"title":"Some Issues of Modern Linguodidactics","authors":"Elyor Imomov Abdikarimovich","doi":"10.17762/jaz.v44is1.2801","DOIUrl":"https://doi.org/10.17762/jaz.v44is1.2801","url":null,"abstract":"This thesis explores the multifaceted nature of linguodidactics, addressing issues related to multilingualism, technology integration, language assessment, teacher training, language policies, inclusivity, motivation, and emerging trends. By examining these issues, this research aims to contribute to the enhancement of language teaching and learning practices, ultimately fostering effective communication and intercultural understanding in an increasingly globalized world.","PeriodicalId":35945,"journal":{"name":"Journal of Advanced Zoology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138971276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. D. Loupatty, Edward J. Dompeipen, M. Radiena, J. P. Kolanus
Seaweed is the key to developing habitat in deep sea, coastal, and estuarine environments. Seaweed, humans, and animals have been interrelated since the beginning, especially as a food source of fiber, protein, vitamins, antioxidants, and bioactive compounds. Natural antioxidants are seen as a superior and safe method of fighting free radicals. Sources of antioxidants usually come from land plants and rarely from seaweed. Silpau is a seaweed and a local food ingredient in Maluku Province, Indonesia, and has the potential as an antioxidant. Antioxidant and bioactive compounds are needed by humans and animals as antibodies to fight various negative influences from the environment. This research aimed to isolate and characterize active antioxidant compounds and the toxicity of silpau extracts. The extraction of the active compound begins with a single maceration method for 3 x 24 hours with methanol solvent, processing with the partition extraction method for subfraction separation using column chromatography with the silica gel stationary phase and the mobile phase solvent n-hexane: ethyl acetate with a ratio (50: 1 ~ 1: 1) gradient. The sample toxicity was tested using the Brine Shrimp Lethality Test method. The trial activity used the Free Radical Reduction method with 2,2-diphenyl-1-pikrilhidrazil as a reagent. This research succeeded in isolating one bioactive compound from the glare green macroalgae. Based on the phytochemical analysis and FT-IR spectrophotometer, sub-fraction IV was identified as a triterpenoid class of compounds that has an IC50 value of 126,582 μg / ml and is considered an antioxidant.
海藻是开发深海、沿海和河口环境栖息地的关键。海藻、人类和动物从一开始就相互关联，尤其是作为纤维、蛋白质、维生素、抗氧化剂和生物活性化合物的食物来源。天然抗氧化剂被认为是对抗自由基的一种优质安全的方法。抗氧化剂的来源通常来自陆地植物，很少来自海藻。Silpau 是一种海藻，也是印度尼西亚马鲁古省的一种当地食材，具有抗氧化剂的潜力。人类和动物都需要抗氧化剂和生物活性化合物作为抗体来对抗环境中的各种负面影响。这项研究旨在分离和鉴定活性抗氧化化合物以及西帕提取物的毒性。首先用甲醇溶剂进行 3 x 24 小时的单次浸渍法提取活性化合物，然后用硅胶固定相和流动相溶剂正己烷：乙酸乙酯比例（50:1 ~ 1:1）梯度的柱层析分区萃取法进行亚馏分分离。样品毒性采用盐水虾致死试验法进行测试。试验活性采用自由基还原法，试剂为 2,2-二苯基-1-pikrilhidrazil。这项研究成功地从眩绿色大型藻类中分离出一种生物活性化合物。根据植物化学分析和傅立叶变换红外分光光度计，子馏分 IV 被确定为一种三萜类化合物，其 IC50 值为 126,582 μg /ml，被认为是一种抗氧化剂。
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Pub Date : 2023-12-14DOI: 10.17762/jaz.v44is1.2802
Yigitaliyeva Muhlisa Ahadovna
This article is devoted to the isomorphic and allomorphic aspect of proverbs in English and Uzbek.
本文专门讨论英语和乌兹别克语中谚语的同构和异构问题。
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