Bulletin de Philosophie Medievale最新文献

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Leuven: “A Question of Life and Death. Living and Dying in Medieval Latin Philosophy, 1200-1500” 鲁汶:《生死问题》《中世纪拉丁哲学的生与死(1200-1500)》

Q3 Arts and Humanities

Bulletin de Philosophie Medievale

Pub Date : 2019-01-01 DOI: 10.1484/j.bpm.5.118709

Tilke Nelis

引用次数: 0

Paris: “Thomas Aquinas on the Human Body. Nature and Destiny” 巴黎:《托马斯·阿奎那论人体》《自然与命运》

Q3 Arts and Humanities

Bulletin de Philosophie Medievale

Pub Date : 2019-01-01 DOI: 10.1484/j.bpm.5.118711

Marta Borgo

引用次数: 0

Unbiased Detection of Somatic Copy Number Aberrations in cfDNA of Lung Cancer Cases and High-Risk Controls with Low Coverage Whole Genome Sequencing. 利用低覆盖率全基因组测序技术无偏见地检测肺癌病例和高危对照组 cfDNA 中的体细胞拷贝数畸变

Q3 Arts and Humanities

Bulletin de Philosophie Medievale

Pub Date : 2016-01-01 DOI: 10.1007/978-3-319-42044-8_6

Fiona Taylor, James Bradford, Penella J Woll, Dawn Teare, Angela Cox

Molecular profiling using low coverage whole genome sequencing of cell free DNA (cfDNA) represents a non-targeted approach to identify multiple somatic copy number alterations (SCNA) across different lung cancer subtypes. We aim to establish that SCNA can be detected in cfDNA of lung cancer cases.Standard protocols were followed to process matched cfDNA, formalin-fixed paraffin embedded (FFPE) tumour and lymphocyte DNA. Copy number profiles for cfDNA or FFPE DNA were normalised to profiles from matched lymphocyte DNA with the software CNAnorm. Technical sensitivity was determined by spiking different proportions of FFPE tumour DNA into cfDNA from controls.The median genome coverage was 0.26X (range 0.05X-0.97X). For two advanced stage cases there was a positive correlation between copy number ratio profiles of matched cfDNA and FFPE DNA (r = 0.62, p < 0.0001 and r = 0.75, p < 0.0001). There was no correlation for four advanced and two early stage cases. There were low magnitude copy number aberrations detected in high-risk controls (N = 5). We detected spiked FFPE DNA derived SCNAs with a tumour fraction as low as 10 % of cfDNA.Our preliminary results demonstrate non-invasive detection of tumour-derived copy number alterations in advanced lung cancer cases with low coverage whole genome sequencing. Clinical characteristics and treatment may influence whether SCNA are detected in cfDNA.

利用细胞游离DNA（cfDNA）的低覆盖率全基因组测序进行分子剖析是一种非靶向方法，可鉴定不同肺癌亚型的多种体细胞拷贝数改变（SCNA）。我们的目标是在肺癌病例的cfDNA中检测到SCNA。我们按照标准协议处理匹配的cfDNA、福尔马林固定石蜡包埋（FFPE）肿瘤和淋巴细胞DNA。用 CNAnorm 软件将 cfDNA 或 FFPE DNA 的拷贝数图谱与匹配的淋巴细胞 DNA 的图谱进行归一化处理。通过在对照组的cfDNA中添加不同比例的FFPE肿瘤DNA来确定技术灵敏度。在两个晚期病例中，匹配的 cfDNA 和 FFPE DNA 的拷贝数比率图谱呈正相关（r = 0.62，p < 0.0001 和 r = 0.75，p < 0.0001）。四个晚期病例和两个早期病例没有相关性。在高危对照组（N = 5）中检测到了低度拷贝数畸变。我们检测到了加标 FFPE DNA 衍生的 SCNA，其肿瘤部分在 cfDNA 中的占比低至 10%。临床特征和治疗可能会影响是否能在cfDNA中检测到SCNA。

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引用次数: 0

Alchemy and medicine in the Middle Ages. 中世纪的炼金术和医学。

Q3 Arts and Humanities

Bulletin de Philosophie Medievale

Pub Date : 1996-01-01 DOI: 10.1484/j.bpm.3.513

C Crisciani

引用次数: 2

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