Background. The manor park in Laskowice (Kuyavian-Pomeranian Voivodeship) was established in naturalistic style in the 19th century. Since then it has been transformed considerably. The aim of this paper was to analyse the structure of the vascular flora before revitalisation of the park and to present proposals of specimens of the dendroflora to be protected as natural monuments. Material and methods. In 2015 and 2016 vascular plants, including monumental specimens of the dendroflora, were inventoried, as had been commissioned by the current administrator of the Dąbrowa Forest Inspectorate. The vascular flora was analysed for life forms, geographical, historical, sociological and ecological groups. Results. There were 203 taxa of the vascular flora in the park, including 8 valuable species – endangered and protected. The Poaceae, Asteraceae and Rosaceae families were the most abundant. There was a large share of alien species in the flora (27%), among which kenophytes were the most abundant. Hemicryptophytes were the predominant life form, followed by phanerophytes, therophytes and geophytes. Species of the following classes of non-forest habitats were predominant: Artemisietea vulgaris, Agropyretea intermedio-repentis and Molinio-Arrhenatheretea. There was a slightly smaller share of species of the Querco-Fagetea class. Both forest and ruderal species pointed to Tilio-Carpinetum as the potential forest community. Conclusions. The large share of alien species in the park flora indicated its anthropogenic character. The inventory of the flora provides the basis for revitalisation of the park with its natural values. 61 tree species and blooming ivy specimens were proposed for legal protection as natural monuments.
{"title":"The Structure of the Vascular Flora in the Manor Park in Laskowice","authors":"I. Paszek, Dorota Gawenda-Kempczyńska, T. Załuski","doi":"10.17306/J.NPT.00204","DOIUrl":"https://doi.org/10.17306/J.NPT.00204","url":null,"abstract":"Background. The manor park in Laskowice (Kuyavian-Pomeranian Voivodeship) was established in naturalistic style in the 19th century. Since then it has been transformed considerably. The aim of this paper was to analyse the structure of the vascular flora before revitalisation of the park and to present proposals of specimens of the dendroflora to be protected as natural monuments. Material and methods. In 2015 and 2016 vascular plants, including monumental specimens of the dendroflora, were inventoried, as had been commissioned by the current administrator of the Dąbrowa Forest Inspectorate. The vascular flora was analysed for life forms, geographical, historical, sociological and ecological groups. Results. There were 203 taxa of the vascular flora in the park, including 8 valuable species – endangered and protected. The Poaceae, Asteraceae and Rosaceae families were the most abundant. There was a large share of alien species in the flora (27%), among which kenophytes were the most abundant. Hemicryptophytes were the predominant life form, followed by phanerophytes, therophytes and geophytes. Species of the following classes of non-forest habitats were predominant: Artemisietea vulgaris, Agropyretea intermedio-repentis and Molinio-Arrhenatheretea. There was a slightly smaller share of species of the Querco-Fagetea class. Both forest and ruderal species pointed to Tilio-Carpinetum as the potential forest community. Conclusions. The large share of alien species in the park flora indicated its anthropogenic character. The inventory of the flora provides the basis for revitalisation of the park with its natural values. 61 tree species and blooming ivy specimens were proposed for legal protection as natural monuments.","PeriodicalId":366305,"journal":{"name":"Nauka Przyroda Technologie","volume":"138 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115079524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Utracone i poszukiwane wartości krajobrazu dolin rzecznych i zieleni śródmiejskiej w Szamotułach","authors":"P. Szumigała, P. Urbański","doi":"10.17306/J.NPT.00208","DOIUrl":"https://doi.org/10.17306/J.NPT.00208","url":null,"abstract":"","PeriodicalId":366305,"journal":{"name":"Nauka Przyroda Technologie","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131329190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Świerczyński, A. Stachowiak, M. Golcz-Polaszewska
{"title":"The influence of treatments applied to common ninebark (Physocarpus opulifolius (L.) Maxim.) ‘Diabolo’ cuttings on their rooting and growth","authors":"S. Świerczyński, A. Stachowiak, M. Golcz-Polaszewska","doi":"10.17306/J.NPT.00184","DOIUrl":"https://doi.org/10.17306/J.NPT.00184","url":null,"abstract":"","PeriodicalId":366305,"journal":{"name":"Nauka Przyroda Technologie","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130327293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Niżnikowski, M. Świątek, Ż. Szymańska, M. Ślęzak
Background. The study compared the body development and slaughter traits of male and female Polish Wrzosówka lambs and their F2 crossbreeds with Berrichon du Cher rams. Material and methods. The study on male and female Polish Wrzosówka lambs and their F2 crossbreeds with Berrichon du Cher rams was conducted at Prof. Adam Skoczylas Research Farm in Żelazna, Poland between 2012 and 2014. The body development of all the lambs was assessed, but only the rams were slaughtered to assess their carcass quality. The carcasses were assessed according to the EUROP grid. The left half-carcasses were divided into cuts. Results. The F2 crossbreeds were characterised by higher body weights and daily gains. Their carcasses contained less fat, had better fat consistency and higher slaughter value, were heavier and contained more valuable cuts than the Polish Wrzosówka sheep. Conclusion. The findings of the research on the development of a new synthetic line of sheep based on the Polish Wrzosówka sheep for the Central Poland market give grounds to initiate the procedure to register the new synthetic line.
背景。本研究比较了波兰Wrzosówka公羊、母羊及其F2杂交母羊与Berrichon du Cher公羊的身体发育和屠宰性状。材料和方法。2012年至2014年,在波兰Żelazna的Adam Skoczylas教授研究农场,对波兰雄性和雌性Wrzosówka羔羊及其与Berrichon du Cher公羊杂交的F2进行了研究。评估所有羔羊的身体发育,但仅屠宰公羊以评估其胴体质量。根据EUROP网格对胴体进行评估。剩下的半具尸体被分成几块。结果。F2杂交品种具有较高的体重和日增重。与波兰Wrzosówka羊相比,它们的胴体脂肪含量更少,脂肪稠度更好,屠宰价值更高,重量更重,切块更有价值。结论。为波兰中部市场开发以波兰Wrzosówka羊为基础的新合成羊系的研究结果为启动新合成羊系的注册程序提供了依据。
{"title":"A Comparison of the Body Development and Slaughter Value of Polish Wrzosówka Sheep and Their F2 Crossbreeds with Berrichon du Cher Sheep","authors":"R. Niżnikowski, M. Świątek, Ż. Szymańska, M. Ślęzak","doi":"10.17306/J.NPT.00212","DOIUrl":"https://doi.org/10.17306/J.NPT.00212","url":null,"abstract":"Background. The study compared the body development and slaughter traits of male and female Polish Wrzosówka lambs and their F2 crossbreeds with Berrichon du Cher rams. Material and methods. The study on male and female Polish Wrzosówka lambs and their F2 crossbreeds with Berrichon du Cher rams was conducted at Prof. Adam Skoczylas Research Farm in Żelazna, Poland between 2012 and 2014. The body development of all the lambs was assessed, but only the rams were slaughtered to assess their carcass quality. The carcasses were assessed according to the EUROP grid. The left half-carcasses were divided into cuts. Results. The F2 crossbreeds were characterised by higher body weights and daily gains. Their carcasses contained less fat, had better fat consistency and higher slaughter value, were heavier and contained more valuable cuts than the Polish Wrzosówka sheep. Conclusion. The findings of the research on the development of a new synthetic line of sheep based on the Polish Wrzosówka sheep for the Central Poland market give grounds to initiate the procedure to register the new synthetic line.","PeriodicalId":366305,"journal":{"name":"Nauka Przyroda Technologie","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125646613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background. The analysis of athletes’ body composition is particularly important for planning nutrition and training loads. Reproducibility and precision of measurements are particularly important in strength sports, when there are fluctuations in the body mass and composition around training. Precise monitoring of these changes with an adequate method enables prediction of the effects of inadequate diet or workload. The aim of the study was to assess body fat (%Fat) measurements with spectrophotometry (NIR), bioelectrical impedance (BIA) and the anthropometric technique (SF) and to compare them with air plethysmography (BP) as a reference method in a group of powerlifters. Material and methods. The study was conducted simultaneously on a group of 49 men aged 19– 26 years, practising classic powerlifting. The body fat content was measured with the Bod Pod (BP method), FUTREX 6100A/ZL (NIR method), Tanita MC 780 MA (BIA method), whereas skin thickness was measured with a Harpenden skinfold calliper (SF technique). Results. The total measurement accuracy coefficient for %Fat was 100% with BIA, 94% with SF and 74% with NIR, as compared with the reference method. In the group with the body fat content ≤ 20% (L-Fat-P) and > 20% (H-Fat-P) the accuracy coefficients were respectively 117 vs. 86% (BIA), 105 vs. 82% (SF) and 84 vs. 63% (NIR). The total correlation coefficients between the methods under analysis and the reference method were significant: 0.70 (SF), 0.61 (BIA), 0.43 (NIR). In the L-Fat-P group there was a significant correlation between the BP and SF methods (0.52), and in the H-Fat-P group – between the BP and BIA methods (0.53). There were relative differences in the total group, L-Fat-P and H-Fat-P between the BP and NIR methods: –20.7% and –9.2% vs. –37.1%, the BIA method: 8.9% and 23.6% vs. –14.2%, and the SF technique: –0.6% and 9.8% vs. –17.1%, respectively. Conclusions. The results indicated the need to select carefully the techniques analysing athletes’ body composition. Fat percentage measurement with the anthropometric technique was the most accurate; it was moderately overestimated when the bioelectrical impedance method was applied, whereas the spectrophotometric method resulted in significant underestimation. The accuracy of the measurements depended on the body fat content. In the future it is necessary to conduct research on endurance athletes as well as people who do not practise sports.
背景。对运动员身体成分的分析对于规划营养和训练负荷尤为重要。在力量运动中,当训练前后身体质量和组成有波动时,测量的可重复性和准确性尤为重要。用适当的方法对这些变化进行精确监测,可以预测饮食或工作负荷不足的影响。本研究的目的是评估用分光光度法(NIR)、生物电阻抗法(BIA)和人体测量技术(SF)测量的体脂(% fat),并将其与作为参考方法的空气体积脉搏图(BP)进行比较。材料和方法。这项研究同时对49名年龄在19 - 26岁之间的男性进行,他们都在练习经典的力量举。体脂含量采用Bod Pod (BP法)、FUTREX 6100A/ZL(近红外法)、Tanita MC 780 MA (BIA法)测定,皮肤厚度采用Harpenden皮褶卡尺(SF技术)测定。结果。与参考方法相比,BIA法测定%Fat的总准确度系数为100%,SF法为94%,NIR法为74%。体脂含量≤20% (L-Fat-P)和> 20% (H-Fat-P)组的准确度系数分别为117比86% (BIA)、105比82% (SF)和84比63% (NIR)。分析方法与参考方法的总相关系数分别为0.70 (SF)、0.61 (BIA)、0.43 (NIR)。在L-Fat-P组中,BP与SF法之间存在显著相关性(0.52),H-Fat-P组中BP与BIA法之间存在显著相关性(0.53)。BP法和NIR法的总组、L-Fat-P和H-Fat-P的相对差异分别为-20.7%和-9.2% vs. -37.1%, BIA法分别为8.9%和23.6% vs. -14.2%, SF法分别为-0.6%和9.8% vs. -17.1%。结论。结果表明,分析运动员身体成分的技术需要谨慎选择。人体测量法测定脂肪百分比最准确;应用生物电阻抗法可适度高估,而分光光度法可显著低估。测量的准确性取决于身体脂肪含量。未来有必要对耐力运动员以及不从事体育运动的人进行研究。
{"title":"A comparative analysis of the accuracy of body fat measurements using spectrophotometry, bioelectrical impedance and anthropometric technique vs. air displacement plethysmography in a group of powerlifters","authors":"M. Człapka-Matyasik, M. Grdeń","doi":"10.17306/J.NPT.00182","DOIUrl":"https://doi.org/10.17306/J.NPT.00182","url":null,"abstract":"Background. The analysis of athletes’ body composition is particularly important for planning nutrition and training loads. Reproducibility and precision of measurements are particularly important in strength sports, when there are fluctuations in the body mass and composition around training. Precise monitoring of these changes with an adequate method enables prediction of the effects of inadequate diet or workload. The aim of the study was to assess body fat (%Fat) measurements with spectrophotometry (NIR), bioelectrical impedance (BIA) and the anthropometric technique (SF) and to compare them with air plethysmography (BP) as a reference method in a group of powerlifters. Material and methods. The study was conducted simultaneously on a group of 49 men aged 19– 26 years, practising classic powerlifting. The body fat content was measured with the Bod Pod (BP method), FUTREX 6100A/ZL (NIR method), Tanita MC 780 MA (BIA method), whereas skin thickness was measured with a Harpenden skinfold calliper (SF technique). Results. The total measurement accuracy coefficient for %Fat was 100% with BIA, 94% with SF and 74% with NIR, as compared with the reference method. In the group with the body fat content ≤ 20% (L-Fat-P) and > 20% (H-Fat-P) the accuracy coefficients were respectively 117 vs. 86% (BIA), 105 vs. 82% (SF) and 84 vs. 63% (NIR). The total correlation coefficients between the methods under analysis and the reference method were significant: 0.70 (SF), 0.61 (BIA), 0.43 (NIR). In the L-Fat-P group there was a significant correlation between the BP and SF methods (0.52), and in the H-Fat-P group – between the BP and BIA methods (0.53). There were relative differences in the total group, L-Fat-P and H-Fat-P between the BP and NIR methods: –20.7% and –9.2% vs. –37.1%, the BIA method: 8.9% and 23.6% vs. –14.2%, and the SF technique: –0.6% and 9.8% vs. –17.1%, respectively. Conclusions. The results indicated the need to select carefully the techniques analysing athletes’ body composition. Fat percentage measurement with the anthropometric technique was the most accurate; it was moderately overestimated when the bioelectrical impedance method was applied, whereas the spectrophotometric method resulted in significant underestimation. The accuracy of the measurements depended on the body fat content. In the future it is necessary to conduct research on endurance athletes as well as people who do not practise sports.","PeriodicalId":366305,"journal":{"name":"Nauka Przyroda Technologie","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127144358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sylwia Marszałkiewicz, A. Siger, Elżbieta Radziejewska -Kubzdela, K. Ratusz, M. Rudzińska
{"title":"The physicochemical properties of cold-pressed camelina seed oils","authors":"Sylwia Marszałkiewicz, A. Siger, Elżbieta Radziejewska -Kubzdela, K. Ratusz, M. Rudzińska","doi":"10.17306/J.NPT.00206","DOIUrl":"https://doi.org/10.17306/J.NPT.00206","url":null,"abstract":"","PeriodicalId":366305,"journal":{"name":"Nauka Przyroda Technologie","volume":"256 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121175262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Dinh, H. Piekarska-Boniecka, Marta Rzańska-Wieczorek
{"title":"Aphidiinae (Hymenoptera, Braconidae) occurring in agricultural environments, forests and urban areas in Poland","authors":"D. Dinh, H. Piekarska-Boniecka, Marta Rzańska-Wieczorek","doi":"10.17306/J.NPT.00197","DOIUrl":"https://doi.org/10.17306/J.NPT.00197","url":null,"abstract":"","PeriodicalId":366305,"journal":{"name":"Nauka Przyroda Technologie","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133627297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of the usefulness of 25 varieties of Heuchera L. as ground covers","authors":"P. Czuchaj, S. Szczepaniak","doi":"10.17306/J.NPT.00221","DOIUrl":"https://doi.org/10.17306/J.NPT.00221","url":null,"abstract":"","PeriodicalId":366305,"journal":{"name":"Nauka Przyroda Technologie","volume":"18 23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131397770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agnieszka Kaźmierska, I. Bolesławska, J. Przysławski
Clinical research showed that skin lesions caused by the deficiency of n-6 fatty acids in the diet can be inhibited by oral administration of oils rich in gamma-linolenic acid (GLA). Gamma-linolenic acid is effective in the treatment of sores, erythema and eczema. It also improves defensive functions of the skin against infections, has anti-inflammatory properties and increases the blood perfusion in skin cells. It improves nutrition, oxygenation of the skin and disposal of toxins. In consequence, the skin colour and appearance improve. The acid is important for skin hydration as its deficiency results in dry skin. The study discusses the influence of n-6 polyunsaturated fatty acids on the functional status of the skin, featuring gamma-linolenic acid.
{"title":"The influence of polyunsaturated fatty acids on the skin, featuring the effect of gamma-linolenic acid","authors":"Agnieszka Kaźmierska, I. Bolesławska, J. Przysławski","doi":"10.17306/J.NPT.00205","DOIUrl":"https://doi.org/10.17306/J.NPT.00205","url":null,"abstract":"Clinical research showed that skin lesions caused by the deficiency of n-6 fatty acids in the diet can be inhibited by oral administration of oils rich in gamma-linolenic acid (GLA). Gamma-linolenic acid is effective in the treatment of sores, erythema and eczema. It also improves defensive functions of the skin against infections, has anti-inflammatory properties and increases the blood perfusion in skin cells. It improves nutrition, oxygenation of the skin and disposal of toxins. In consequence, the skin colour and appearance improve. The acid is important for skin hydration as its deficiency results in dry skin. The study discusses the influence of n-6 polyunsaturated fatty acids on the functional status of the skin, featuring gamma-linolenic acid.","PeriodicalId":366305,"journal":{"name":"Nauka Przyroda Technologie","volume":"57 s74","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113957205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iuliia Ielchishcheva, B. Stachowiak, Artur Szwengiel, A. Bozhkov
Background. The basidiomycete Rhodosporidium diobovatum is an oleaginous yeast capable of carotenoid synthesis. Scientific research on this yeast indicates that it can be a promising industrial producer of this pigment. The yeast is capable of assimilating numerous carbon substrates and it can grow under various, often extreme, environmental conditions. Wild strains can be isolated from soil, the depths of the sea and the surface of plants. They can grow both in the dark and in the presence of light. The aim of this study was to determine the capability of the Rh. diobovatum IMB Y-5023 yeast strain to produce and accumulate carotenoids when cultured on media with different carbohydrate concentration and composition and with low monosaccharide levels. The yeast was cultured at different illuminances. This research is of great theoretical and practical importance, because it may enable the use of various organic by-products from the agri-food industry as substrates for culturing Rh. diobovatum and for microbial production of carotenoids. This research also provides an opportunity to reduce the costs of the microbiological process by choosing optimal illuminance of the culture. Material and methods. Rhodosporidium diobovatum IMB Y-5023 was cultured on three test media: CM (carrot medium), BYP (wheat bran extract), and the YM medium with higher concentration of malt extract as the main source of oligosaccharides and with maltotriose added. Cultures were carried out at 22°C for 5 days at illuminance ranging from 0 to 5,000 lx. The carbohydrate composition of the media was determined before and after the culturing of Rh. diobovatum IMB Y-5023 by means of UHPLC coupled with tandem mass spectrometry (MS). The following parameters were also measured after culturing: biomass yield (DCW – dry cell weight, g/l), the concentration of carotenoids in the yeast biomass (mg/g DCW), the biomass yield coefficient (YX/S) Ielchishcheva, Iu., Stachowiak, B., Szwengiel, A., Bozhkov, A. (2017). Carbohydrate components of culture media as determinants of the Rhodosporidium diobovatum IMB Y-5023 yeast metabolism. Nauka Przyr. Technol., 11, 3, 291–303. http://dx.doi.org/10.17306/J.NPT.00210 292 and the carotenoid yield coefficient (YC/S). The samples were compared by means of cluster analysis (CA) and principal component analysis (PCA), which were used to visualise the relationships between the study variables and the samples. Results. The Rh. diobovatum IMB Y-5023 yeast strain grew and produced carotenoids on all the media. It assimilated the DP3 and DP4 oligosaccharides contained in the media. Regardless of the illuminance during culturing, the consumption of sugars in the test media remained at 90% or more. However, the concentration and type of carbohydrates in the test medium affected the course of their metabolism. Regardless of the illuminance, the highest concentration of pigments in the cells and the lowest yield of biomass were noted with YM. The initial concentr
{"title":"Carbohydrate components of culture media as determinants of the Rhodosporidium diobovatum IMB Y-5023 yeast metabolism","authors":"Iuliia Ielchishcheva, B. Stachowiak, Artur Szwengiel, A. Bozhkov","doi":"10.17306/J.NPT.00210","DOIUrl":"https://doi.org/10.17306/J.NPT.00210","url":null,"abstract":"Background. The basidiomycete Rhodosporidium diobovatum is an oleaginous yeast capable of carotenoid synthesis. Scientific research on this yeast indicates that it can be a promising industrial producer of this pigment. The yeast is capable of assimilating numerous carbon substrates and it can grow under various, often extreme, environmental conditions. Wild strains can be isolated from soil, the depths of the sea and the surface of plants. They can grow both in the dark and in the presence of light. The aim of this study was to determine the capability of the Rh. diobovatum IMB Y-5023 yeast strain to produce and accumulate carotenoids when cultured on media with different carbohydrate concentration and composition and with low monosaccharide levels. The yeast was cultured at different illuminances. This research is of great theoretical and practical importance, because it may enable the use of various organic by-products from the agri-food industry as substrates for culturing Rh. diobovatum and for microbial production of carotenoids. This research also provides an opportunity to reduce the costs of the microbiological process by choosing optimal illuminance of the culture. Material and methods. Rhodosporidium diobovatum IMB Y-5023 was cultured on three test media: CM (carrot medium), BYP (wheat bran extract), and the YM medium with higher concentration of malt extract as the main source of oligosaccharides and with maltotriose added. Cultures were carried out at 22°C for 5 days at illuminance ranging from 0 to 5,000 lx. The carbohydrate composition of the media was determined before and after the culturing of Rh. diobovatum IMB Y-5023 by means of UHPLC coupled with tandem mass spectrometry (MS). The following parameters were also measured after culturing: biomass yield (DCW – dry cell weight, g/l), the concentration of carotenoids in the yeast biomass (mg/g DCW), the biomass yield coefficient (YX/S) Ielchishcheva, Iu., Stachowiak, B., Szwengiel, A., Bozhkov, A. (2017). Carbohydrate components of culture media as determinants of the Rhodosporidium diobovatum IMB Y-5023 yeast metabolism. Nauka Przyr. Technol., 11, 3, 291–303. http://dx.doi.org/10.17306/J.NPT.00210 292 and the carotenoid yield coefficient (YC/S). The samples were compared by means of cluster analysis (CA) and principal component analysis (PCA), which were used to visualise the relationships between the study variables and the samples. Results. The Rh. diobovatum IMB Y-5023 yeast strain grew and produced carotenoids on all the media. It assimilated the DP3 and DP4 oligosaccharides contained in the media. Regardless of the illuminance during culturing, the consumption of sugars in the test media remained at 90% or more. However, the concentration and type of carbohydrates in the test medium affected the course of their metabolism. Regardless of the illuminance, the highest concentration of pigments in the cells and the lowest yield of biomass were noted with YM. The initial concentr","PeriodicalId":366305,"journal":{"name":"Nauka Przyroda Technologie","volume":" 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133017159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}