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Phenotypic Characteristics of Shahi Strain of Nagpuri Buffaloes in Chandrapur District of Maharashtra, India 印度马哈拉施特拉邦Chandrapur地区那格普里水牛Shahi品系的表型特征
Q2 Veterinary Pub Date : 2020-03-09 DOI: 10.6000/1927-520x.2019.08.03.4
H. S. Hamand, R. Zinjarde, S. N. Rokde, N. Chore
: Phenotypic Characterization of Shahi Strain of Nagpuri Buffaloes was done in the Chandrapur District of Maharashtra, India. Two hundred and forty Shahi strain of Nagpuri female buffaloes of various age groups were observed in their home tract (n farms=24). It was found that black coat colour, black coloured muzzle, white patches on the legs, whitish eyes, white coloured tail switch, horizontal ear orientation, sword-shaped horns with pointed tip first turning backwards and then upwards, bowl-shaped udder are typical characteristics of this breed. The average live body weight, body length, chest girth, height at withers, head length, breadth of head of these buffaloes is (means ± standard deviation) 433 ± 8.4 kg, 137.08 ± 2.78 cm 180.81 ± 3.84 cm, 129.43 ± 2.26 cm, 54.18 ± 1.10 cm, 28.18 ± 0.34 cm in 3 years and above age group, respectively. The average daily milk yield and milk yield per lactation of Shahi strain of Nagpuri buffaloes were observed to be 4.06 ± 0.08 kg and 1,220.84 ± 41.57 kg, respectively.
在印度马哈拉施特拉邦Chandrapur地区对纳格普里水牛Shahi菌株进行了表型鉴定。在各年龄段的那格普里雌水牛240头(n个养殖场=24个)中进行了观察。研究发现,黑色的毛色,黑色的口吻,腿上的白色斑点,白色的眼睛,白色的尾巴开关,水平的耳朵方向,剑状的角,尖端先向后转,然后向上,碗状的乳房是该品种的典型特征。3岁及以上年龄组的平均活重、体长、胸围、肩高、头长、头宽分别为(均值±标准差)433±8.4 kg、137.08±2.78 cm、180.81±3.84 cm、129.43±2.26 cm、54.18±1.10 cm、28.18±0.34 cm。纳格普里沙希系水牛平均日产奶量为4.06±0.08 kg,单次泌乳量为1220.84±41.57 kg。
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引用次数: 0
Monoclonal Antibodies Specific to Water Buffalo (Bubalus bubalis) Myxovirus Resistance Protein_1 水牛黏液病毒抗性蛋白_1的单克隆抗体
Q2 Veterinary Pub Date : 2020-02-20 DOI: 10.6000/1927-520x.2020.09.03
Dam Van Phai, V. Sophie, Dubois Axel, Bui Tran Anh Dao, Desmecht Daniel
: Recombinant bubaline ( Bubalus bubalis ) myxovirus resistance protein 1 (bbMx1) was successfully expressed by an Escherichia coli expression system. After immunization and cell fusion, a set of ten mouse hybridomas producing mAbs to bbMx1 was established. The ten corresponding mAbs were further characterized using indirect ELISA, western blot analysis and immunocytofluorescent staining. Eight mAbs, designated 11C7, RD5, NF3, 9D1, FD4, PE6, 11A7 and 10F5, displayed binding abilities and specificity in the three formats. Moreover, combining NF3 (for capture) with RD5 (for detection), 9D1 with RD5, FD4 with 11C7 or PE6 with 11C7 generated a strong signal in a prototype sandwich-ELISA. The results suggest that the mAbs developed and characterized here provide an excellent starting point for developing diagnostic tools aimed at detecting viral infections in the water buffalo, whatever using immunoblotting, immunocytostaining or sandwich-ELISA.
:利用大肠杆菌表达系统成功表达了重组bubaline (Bubalus bubalis)黏液病毒抗性蛋白1 (bbMx1)。免疫和细胞融合后,建立了一组10只产生bbMx1单克隆抗体的小鼠杂交瘤。采用间接ELISA、western blot和免疫细胞荧光染色对10个对应的单抗进行进一步鉴定。8个单克隆抗体分别为11C7、RD5、NF3、9D1、FD4、PE6、11A7和10F5,在三种格式下均显示出结合能力和特异性。此外,将NF3(用于捕获)与RD5(用于检测)、9D1与RD5、FD4与11C7或PE6与11C7结合,在原型三明治- elisa中产生强信号。结果表明,本文开发和表征的单克隆抗体为开发用于检测水牛病毒感染的诊断工具提供了一个很好的起点,无论是使用免疫印迹法、免疫细胞染色法还是三明治elisa法。
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引用次数: 0
Scanning and Transmission Electron-Microscopic Studies on the Lingual Tonsil of the Buffalo (Bubalus bubalis) 水牛舌扁桃体的扫描透射电镜研究
Q2 Veterinary Pub Date : 2020-02-11 DOI: 10.6000/1927-520x.2020.09.02
Pawan Kumar, I. Girgiri
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引用次数: 0
Histological and Histochemical Studies on the Lingual Tonsil of the Buffalo (Bubalus bubalis) 水牛(Bubalus bubalis)舌扁桃体的组织学和组织化学研究
Q2 Veterinary Pub Date : 2019-12-31 DOI: 10.6000/1927-520x.2019.08.03.3
I. Girgiri, Pawan Kumar
: The study was conducted on lingual tonsil of six adult buffaloes (5-6 years of age) of the local mixed breed to explore histomorphological characteristics with functional significance. The mucosal surface of the lingual tonsil lined by stratified squamous keratinized epithelium was comprised of strata basale, spinosum, granulosum and corneum whereas towards the deeper folds the epithelium was stratified squamous non-keratinized. The latter epithelium modified into reticular epithelium especially toward the deeper portion and was characterized by a reduced number of epithelial cells, loss of distinct strata and heavy infiltration of lymphoid cells. The propria submucosa had loose irregular connective tissue along with glandular and lymphoid tissue. The lymphoid tissue was mainly distributed in the form of lymphoid follicles and diffused arrangement. The follicles of varying shapes and size showed darkly stained corona and lightly stained germinal centre were surrounded by parafollicular areas. The parafollicular areas possessed high endothelial venules (HEVs) with large sized endothelial cells having round to oval nuclei with distinctly visible centric or eccentric nucleoli. These HEVs are involved in trafficking of lymphocytes by transendothelial and interendothelial migration. The mucous glandular acini presented strong reactions for glycogen, acidic mucopolysaccharides, weakly sulfated mucosubstances, hyaluronic acid, sialomucins and mucins as demonstrated by different histochemical techniques. The secretions of acini also showed the presence of more than 4% content of cysteine. The presence of modified reticular epithelium and associated lymphoid tissue suggested that the lingual tonsil may be involved in sampling of oral antigens and transporting them to the underlying mucosal lymphoid tissue for processing and initiation of immune responses. The future microbiological studies may exploit the tonsil as a targeted organ for improved delivery of existing mucosal vaccines and development of new strategies for oral vaccines.
:本研究对6头当地杂交种成年水牛(5~6岁)的舌扁桃体进行了研究,以探讨其具有功能意义的组织形态学特征。舌扁桃体的粘膜表面由复层鳞状角化上皮组成,包括基底层、棘层、颗粒层和角质层,而朝向较深的褶皱,上皮为复层鳞状非角化上皮。后一种上皮改性为网状上皮,尤其是向较深的部分,其特征是上皮细胞数量减少、明显层丢失和淋巴细胞大量浸润。黏膜下层固有层有疏松的不规则结缔组织以及腺体和淋巴组织。淋巴组织主要以淋巴滤泡和扩散排列的形式分布。不同形状和大小的卵泡显示出染色较深的冠,染色较浅的生发中心被滤泡旁区域包围。滤泡旁区域具有高内皮小静脉(HEVs),大尺寸内皮细胞具有圆形至椭圆形细胞核,核仁明显可见中心或偏心。这些HEV通过跨内皮和内皮间迁移参与淋巴细胞的运输。不同的组织化学技术显示,粘液腺腺泡对糖原、酸性粘多糖、弱硫酸化粘物质、透明质酸、唾液酸和粘蛋白表现出强烈的反应。腺泡分泌物中半胱氨酸含量也超过4%。修饰的网状上皮和相关淋巴组织的存在表明,舌扁桃体可能参与口腔抗原的采样,并将其运输到下方的粘膜淋巴组织,以处理和启动免疫反应。未来的微生物学研究可能会利用扁桃体作为靶器官,改善现有粘膜疫苗的递送,并开发新的口服疫苗策略。
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引用次数: 1
Buffalo Milk Yield, Quality, and Marketing in Different Agro-Climatic Districts of Bangladesh 孟加拉国不同农业气候区的水牛奶产量、质量和销售
Q2 Veterinary Pub Date : 2019-12-31 DOI: 10.6000/1927-520x.2019.08.03.2
S. Rahman, M. Islam, M. Harun-ur-Rashid, N. R. Sarker, M. Siddiki, M. Islam, M. Islam
The study was aimed at assessing the productive performance of dairy buffalo and milk marketing approaches in different agro-climatic districts of Bangladesh. Three (03) districts of Bangladesh viz. Bhola, Mymensingh, and Dinajpur were chosen from the coastal, river basin and semi-arid region, respectively. A triangulation method of survey was used to collect the data and the components of the triangle were buffalo farms, buffalo farmers and buffalo herdsmen. The investigation duration was twelve (12) months. The study revealed that the highest milk yield (5 L/h/d; p=0.010) was found in the river basin and semi-arid region. Lactation yield was also recorded double in the river basin and semi-arid districts compared to coastal districts (p=0.000). In the case of lactation length, the river basin buffaloes possessed 33 and 36% longer than coastal and semi-arid districts, respectively. All the chemical components were found significantly different (p≤0.050) but fat. Among different agro-climatic districts, about 92% of milk was traded in the coastal region after meeting the household's need but it was noted that the farmers from the semi-arid region kept more than 21% of milk for family consumption (p=0.000). The highest unit price (BDT 72/L) of milk was observed in the river basin district (p=0.011). In conclusion, the current situations of buffalo farming and milk marketing approaches in Bangladesh, varies considerably.
该研究旨在评估孟加拉不同农业气候区水牛的生产性能和牛奶营销方法。孟加拉国的三(03)个地区,即Bhola、Mymensingh和Dinajpur,分别来自沿海、流域和半干旱地区。采用三角测量法收集数据,三角形的组成部分是水牛养殖场、水牛养殖户和水牛牧民。调查持续时间为十二(12)个月。研究表明,河流流域和半干旱地区的产奶量最高(5L/h/d;p=0.010)。与沿海地区相比,流域和半干旱地区的泌乳量也翻了一番(p=0.000)。就泌乳长度而言,流域水牛分别比沿海和半干旱区长33%和36%。除脂肪外,所有化学成分均存在显著差异(p≤0.050)。在不同的农业气候区中,约92%的牛奶在满足家庭需求后在沿海地区进行交易,但值得注意的是,半干旱地区的农民保留了21%以上的牛奶供家庭消费(p=0.000)。河流流域的牛奶单价最高(72孟加拉塔卡/升)(p=0.011)。总之,孟加拉国水牛养殖和牛奶营销方法的现状差异很大。
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引用次数: 7
Ultrasonographic Evaluation of Teat Structure and Detection of Prominent Annular Folds in Brazilian Dairy Buffaloes 巴西乳水牛乳头结构的超声评价及明显环状褶皱的检测
Q2 Veterinary Pub Date : 2019-11-09 DOI: 10.6000/1927-520x.2019.08.03.1
L. Bittner, Clinic for Ruminants, R. D. Ollhoff, J. D. Neto, J. Spilke, F. C. Pogliani, Jose L. Martinez, A. Starke
Udder and teat characteristics differ between buffaloes ( Bubalus bubalis ) and cattle ( Bos taurus ) and the differences are important in relation machine milk ability. Dairy buffalo milk out more slowly and are more difficult to milk than dairy cows, especially when milking machines are used. The goal of this study was to determine the ultrasonographic characteristics of udder and teat morphology in dairy buffaloes in Brazil, a country with a relatively recent history of buffalo farming. External assessment of the udder type and ultrasonography of the teat were done in 63 purebred Murrah and Mediterranian ´ Murrah dairy buffalo cows on three farms with different management systems. Udder types differed among farms; the rear-heavy udder type was most prevalent on two farms and the ball-shaped type occurred frequently on the third farm. Ultrasonographic measurements of the teat were made on one farm and showed correlations between the width of the teat tip and teat canal length, between the width of the teat tip and teat wall thickness and between the width of the teat tip and teat length. During the ultrasonographic examination of the teat, hyperechogenic membrane-like structures in the region of the annular folds, referred to as prominent annular folds (pAFs), were detected in at least one teat in 98 % of all buffaloes. These folds caused narrowing of the lumen between the teat cistern and gland cistern, but their physiological function and relevance concerning machine milk ability remain unclear. Further studies are needed to evaluate the effect of prominent annular folds on milk flow characteristics during mechanical milking in dairy buffaloes.
水牛(Bubalus bubalis)和牛(Bos taurus)的乳房和乳房特征不同,这些差异对机器挤奶能力的关系很重要。水牛奶出得慢,比奶牛更难挤,尤其是在使用挤奶机的时候。本研究的目的是确定巴西乳牛乳房和乳头形态的超声特征,巴西是一个水牛养殖历史相对较近的国家。在三个不同管理制度的农场,对63头纯种默拉和地中海默拉水牛进行了乳房类型的外部评估和乳房超声检查。不同农场的乳房类型不同;后重乳型在两个养殖场最为常见,球状乳型在第三个养殖场较为常见。在一个农场进行了乳头超声测量,显示了乳头宽度与乳管长度、乳头宽度与乳壁厚度以及乳头宽度与乳长之间的相关性。在乳头的超声检查中,在98%的水牛中至少在一个乳头中检测到环状褶皱区域的高回声膜样结构,称为突出环状褶皱(paf)。这些褶皱导致乳池和腺池之间的管腔变窄,但其生理功能及其与机器泌乳能力的相关性尚不清楚。需要进一步的研究来评估明显的环状褶皱对奶牛机械挤奶过程中奶流特性的影响。
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引用次数: 1
Effect of Dietary Supplementation of Rumen Undegradable Protein on Productive Performance of Early Lactating Buffaloes 日粮添加瘤胃不分解蛋白对早泌乳水牛生产性能的影响
Q2 Veterinary Pub Date : 2019-09-03 DOI: 10.6000/1927-520x.2019.08.02.4
Shraddhesh Kumar Tiwari, Nawalpur Nepal Madhyabindu Municipality, R. Shah, D. Nepali, M. Tariq, K. P. Acharya, Budhanilakantha Kathmandu Nepal Animal Quarantine Office
An experiment was conducted to study the effect of dietary supplementation of rumen un-degradable protein on productive performance of early lactating buffaloes for the duration of 90 days from February 5, 2014, to May 5, 2014. A total of twenty one lactating Murrah buffaloes in their early to mid-lactation were randomly selected and divided into three groups. Experimental diets were formulated into three- low (30.56% of dietary Crude Protein), medium (43.54% of dietary crude protein) and high (50.04% of dietary CP), iso-nitrogenous (16% CP) and iso-caloric (72% total digestible nutrients) rumen un-digestible protein (RUP) levels. A significant difference (p 0.05). Hence, it can be concluded that the diet containing a medium level of dietary CP as RUP improved the productive performance of early lactating buffaloes. Thus, diet containing the medium level of dietary CP as RUP should be given to improve the productive performance of early lactating buffaloes under the climatic settings of Nepal.
在2014年2月5日至2014年5月5日的90天内,研究了日粮中添加瘤胃不可降解蛋白对早期泌乳水牛生产性能的影响。随机选择21头泌乳早期至中期的Murrah水牛,分为三组。试验日粮分为低瘤胃不消化蛋白(占日粮粗蛋白的30.56%)、中瘤胃不消化蛋白质(占日料粗蛋白的43.54%)和高瘤胃不消化消化蛋白(CP的50.04%)、等氮(CP的16%)和等热量(总可消化营养素的72%)三个水平。有显著性差异(p0.05)。因此,可以得出结论,含有中等水平的CP作为RUP的日粮提高了早期泌乳水牛的生产性能。因此,在尼泊尔的气候条件下,应给予含有RUP等中等水平CP的日粮,以提高早期泌乳水牛的生产性能。
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引用次数: 0
Impact of the Iraq Marshlands Restoration Program on Livestock Population and Production in the Southern Marshes of Iraq 伊拉克沼泽地恢复计划对伊拉克南部沼泽地畜牧业人口和生产的影响
Q2 Veterinary Pub Date : 2019-09-03 DOI: 10.6000/1927-520x.2019.08.02.1
Asaad Y. Ayied, P. Reiss
: The USAID Iraq Marshlands Restoration Program (IMRP) promoted the application of two promising initiatives tested from 2004 to 2006: (1) the introduction of alfalfa and maize fodder to feed and improve the nutrition of buffalo and (2) the creation of a veterinary service to increase livestock production and health. The livestock sector in the marshlands has seen dramatic improvement since IMRP ended in 2006. These changes are across the board in the absolute number of livestock (from 66,700 heads in 2006 to 111,525 heads in 2017) and in their increasing diversity (buffalo were 74.33% of all livestock in 2006 but decreased to 70.33% in 2017). Buffalo, specifically, had greater birth weight (40 kg in 2006 and 45.6 kg in 2017), greater daily growth rates (0.3 kg in 2006 and 1-1.25 kg in 2017), higher daily milk yields (8.81 kg/day in 2006 and 9.69 kg/day in 2017), and longer lactation periods (150 days in 2006 and 238 days in 2017). There have been accompanying advances in the closely related meat and dairy markets. Live weight and market prices for buffalo and cattle have increased three-to four-fold. Rising daily milk yields also sparked the creation of cottage industries to produce dairy products using higher fat buffalo milk. These changes all point to an increasingly healthy and dynamic livestock sector in the marshlands. However, other anticipated changes in the marshlands did not meet expectations. In 2006, IMRP predicted a strong shift away from the historical dominance of buffalo ownership to a more diversified pattern of ownership that included the relatively newly introduced cattle and a rapid increase in sheep holdings, making the latter the dominate animal. Instead, buffaloes have continued to be the vastly most numerous animals owned in the marshlands. In 2017, they ranged from 65 to 75% of all livestock holdings. Greater access to reflooded marshes by buffalo herders and increased financial earnings realized from meat and dairy production may help to explain the continued reliance on buffalo ownership. IMRP’s introduction of a veterinary service dedicated to working in the marshlands villages was far less successful but highly regarded by Marsh Arabs, being the first time that the services were offered in the marshes.
美国国际开发署伊拉克沼泽地恢复计划(IMRP)促进了2004年至2006年试行的两项有希望的倡议的应用:(1)引进苜蓿和玉米饲料,以喂养和改善水牛的营养;(2)建立兽医服务,以增加牲畜生产和健康。自2006年IMRP结束以来,沼泽地的畜牧业有了显著改善。这些变化体现在牲畜的绝对数量(从2006年的66700头增加到2017年的111525头)和牲畜多样性的增加(2006年水牛占所有牲畜的74.33%,但在2017年下降到70.33%)。具体来说,水牛的出生体重较大(2006年为40公斤,2017年为45.6公斤),日生长率较高(2006年为0.3公斤,2017年为1-1.25公斤),日产奶量较高(2006年为8.81公斤/天,2017年为9.69公斤/天),泌乳期较长(2006年为150天,2017年为238天)。与此密切相关的肉类和乳制品市场也出现了相应的进步。水牛和牛的活重和市场价格增加了三到四倍。日牛奶产量的增加也促使了家庭手工业的兴起,他们使用高脂肪的水牛奶生产乳制品。这些变化都表明,沼泽地的畜牧业日益健康和充满活力。然而,沼泽地的其他预期变化没有达到预期。2006年,IMRP预测将从历史上以水牛为主导的所有制向更多样化的所有制模式转变,其中包括相对较新引进的牛和绵羊的快速增长,使后者成为主导动物。相反,水牛仍然是沼泽地中数量最多的动物。2017年,它们占所有牲畜存栏量的65%至75%。水牛牧民更容易进入被洪水淹没的沼泽,以及肉类和乳制品生产带来的经济收入增加,可能有助于解释人们对水牛所有权的持续依赖。IMRP引入了专门在沼泽地村庄工作的兽医服务,远没有那么成功,但受到沼泽阿拉伯人的高度评价,这是第一次在沼泽地提供服务。
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引用次数: 2
Anatomical Structure of Caudal Venae Cavae and its Anastomosis with Hepatic Veins in the Buffalo (Buballus Bubalis) 水牛尾腔静脉的解剖结构及其与肝静脉的吻合
Q2 Veterinary Pub Date : 2019-09-03 DOI: 10.6000/1927-520x.2019.08.02.3
H. Karimi, G. Ardalani, Alireza Mozzaffari
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引用次数: 0
Seasonal Variation of Calving in Murrah Buffalo in Bihar 比哈尔邦默拉水牛产犊的季节变化
Q2 Veterinary Pub Date : 2019-09-03 DOI: 10.6000/1927-520x.2019.08.02.2
V. Potdar, J. Khadse, S. Joshi, Y. Gaundare, N. Phadke, M. Swaminathan, A. Pande
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引用次数: 0
期刊
Journal of Buffalo Science
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