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First report of the Fusarium arthrosporioides/avenaceum complex causing olive fruit rot in Italy 节孢镰刀菌/燕麦复合体在意大利引起橄榄果实腐烂的首次报告
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12198
F. Brugneti, S. Turco, M. I. Drais, I. Giubilei, A. Mazzaglia
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引用次数: 0
First report of Cocksfoot mottle virus infecting wheat in Estonia 爱沙尼亚小麦感染鸡瘟斑驳病毒的首个报告
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12206
M. Sõmera, P. Sooväli
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引用次数: 0
Aspergillus niger causing fruit rot disease on rose apple in Malaysia 马来西亚玫瑰苹果果腐病的黑曲霉
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12216
J. R. Al‐Obaidi, N. Rahmad, N. M. Hanafi
The rose apple (Syzygium samarangense) is a tropical fruit native to Southeast Asia. Rose apple cultivation occupies c. 3,300 hectares of land in Malaysia with an annual production value exceeding US$9 million (Al-Obaidi et al., 2018). However, fruit rot disease results in the loss of approximately one-third of annual production, causing significant economic hardship. Between 2017 to 2020, rose apples in many farms located in Johor, Malaysia displayed symptoms of fruit rot, characterised by dark, sunken circular lesions on ripening fruits (Figure 1). To identify the causal agent, infected fruit tissues (c. 5 × 5 mm2) were surface sterilised using 1.5% sodium hypochlorite for one minute followed by 70% ethanol for another minute. The tissue was then rinsed with sterile distilled water and placed onto potato dextrose agar (PDA). Plates were incubated at 25°C for seven days and colonies grew that initially appeared white, then turned dark green (Figure 2). Based on the morphological characteristics, particularly the range of conidia sizes (3.0 to 5.4 μm), shape (ellipsoidal) and the colour, the fungus was identified as Aspergillus niger (Silva et al., 2011; Li et al., 2020). For molecular confirmation, the internal transcribed spacer (ITS) region was amplified using ITS1 and ITS4 primers (Santos et al., 2016). The PCR amplicon was sequenced (GenBank Accession No. MF362179) and shared 100% identity with A. niger (MG228418.1, MW188561.1 and KY378943.1). To confirm the pathogenicity of the A. niger isolate (ABI-JJ2), healthy, detached rose apple fruits were surface sterilised by immersing them in a 6% sodium hypochlorite solution for five minutes, followed by washing with 70% ethanol for one minute. Wounded fruits (pin-pricked) were then inoculated with agar plugs containing mycelium of A. niger. Control fruits were inoculated with sterile PDA plugs. Four fruits were used for each treatment. The fruits were placed in plastic boxes and incubated at 25°C with a relative humidity of 80% for seven days. Three days after inoculation, all the inoculated fruits exhibited typical Aspergillus fruit rot symptoms, resembling those seen in the field. The control fruits were unaffected and showed no symptoms. Aspergillus niger was reisolated from the lesions on the inoculated fruit, fulfilling Koch's postulates. Aspergillus niger is responsible for widespread diseases in many plants and is frequently found as a contaminant in food (Lima et al., 2019). This study demonstrates the virulence of A. niger on rose apple, resulting in reduced fruit quality and production. To our knowledge, this is the first record of A. niger causing fruit rot on rose apples in Malaysia, and globally. More research is needed into the pathogenicity and epidemiology of A. niger on rose apples, to aid the development of targeted solutions and limit the effects of this disease. The authors would like to thank the National Institutes of Biotechnology Malaysia and Universiti Pendidikan Sultan I
玫瑰苹果(Syzygium samarangense)是一种原产于东南亚的热带水果。马来西亚玫瑰苹果种植面积约为3300公顷,年产值超过900万美元(al - obaidi et al., 2018)。然而,果腐病导致大约三分之一的年产量损失,造成严重的经济困难。在2017年至2020年期间,马来西亚柔佛的许多农场的玫瑰苹果都出现了水果腐烂的症状,其特征是成熟果实上的深色凹陷圆形病变(图1)。为了确定致病因子,用1.5%次氯酸钠对受感染的水果组织(c. 5 × 5 mm2)表面消毒一分钟,然后用70%乙醇再消毒一分钟。然后用无菌蒸馏水冲洗组织,并放置在马铃薯葡萄糖琼脂(PDA)上。培养皿在25°C下孵育7天,菌落生长,最初呈现白色,然后变成深绿色(图2)。根据形态学特征,特别是分生孢子大小(3.0至5.4 μm)、形状(椭球形)和颜色的范围,该真菌被鉴定为黑曲霉(Silva et al., 2011;Li等人,2020)。为了进行分子鉴定,使用ITS1和ITS4引物扩增内部转录间隔区(ITS) (Santos et al., 2016)。PCR扩增子测序(GenBank登录号:;MF362179),与A. niger (MG228418.1, MW188561.1和KY378943.1)具有100%的同源性。为了确认黑黑杆菌分离物(abijj2)的致病性,将健康的分离玫瑰苹果果实在6%次氯酸钠溶液中浸泡5分钟,然后用70%乙醇洗涤1分钟,对其进行表面消毒。然后用含有黑曲霉菌丝体的琼脂塞接种伤果(针刺)。对照果实用无菌PDA插头接种。每个处理使用4个水果。将果实置于塑料箱中,在25°C、相对湿度80%的条件下培养7天。接种3 d后,所有接种的果实均表现出典型的曲霉腐病症状,与田间观察结果相似。对照果实不受影响,没有表现出任何症状。黑曲霉从接种过的果实的损伤处重新分离出来,实现了科赫的假设。黑曲霉(Aspergillus niger)是许多植物广泛病害的罪魁祸首,经常被发现是食品中的污染物(Lima et al., 2019)。本研究证实了黑曲霉对玫瑰苹果的毒力,导致果实品质和产量下降。据我们所知,这是黑曲霉在马来西亚乃至全球首次引起玫瑰苹果果实腐烂的记录。需要对黑曲霉对玫瑰苹果的致病性和流行病学进行更多的研究,以帮助开发有针对性的解决方案并限制这种疾病的影响。作者要感谢马来西亚国立生物技术研究所和Pendidikan Sultan Idris大学在本研究期间提供的帮助。
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引用次数: 0
First report of a ‘Candidatus Phytoplasma aurantifolia’ (16SrII‐D) isolate associated with little leaf and stunting of Dracaena reflexa in India 在印度首次报道一种与龙血树小叶和发育迟缓有关的“Candidatus PhytopPlasma aurantifolia”(16SrII-D)分离物
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12194
K. Arunodhayam, N. Mamatha, K. Gopal, G. Sandhya, S. Susmitha, B. Srinivasulu, D. Naik, M. Reddy, B. V. B. Reddy
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引用次数: 0
First report of Alternaria alternata causing bud and blossom blight on olive in Bosnia and Herzegovina 在波斯尼亚和黑塞哥维那首次报道引起橄榄芽和花枯萎病的互花疫病
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12214
A. Crnogorac, A. Mandić, S. Godena, E. Petrović, S. Matić
During 2020 and 2021, symptoms of withering on olive (Olea europaea) followed by necrosis on buds and flowers were observed in the Ljubuški and Mostar regions of western Herzegovina. These symptoms were observed in two of the ten orchards surveyed and on 83 olive (cv. Oblica) trees in the affected orchards (Figure 1), reaching a disease incidence of 60%. Small pieces from lesion margins were plated on potato dextrose agar and after five days' incubation at 25°C under a 12 hr light/dark regime, a single fungus consistently developed. The 10-day-old monoconidial cultures of two isolates (FAZ HZ and KRA HZ) were plated on potato carrot agar (PCA) and DRYES medium for morphological characterisation (Andersen et al., 2001; Simmons, 2007). Developed fungal colonies were olivaceous and cultures grown on PCA produced brown, branched and septate conidiophores with ellipsoid or ovoid conidia. Mean conidial size was 24.8 ±1.4 × 10.1 ±0.6 μm (Figure 2). Observed morphological features identified the fungus as Alternaria section alternata (Woudenberg et al., 2015). Fungal DNA from two isolates was amplified by PCR using primers for ITS (White et al., 1990), rpb2, endoPG, and Alt a 1 (Woudenberg et al., 2015). Amplicons of 540 bp (ITS), 904 bp (rpb2), 460 bp (endoPG), and 492 bp (Alt a 1) were produced from the FAZ HZ and KRA HZ isolates and sequenced (GenBank Accession Nos. OP010267 and OP010268 for ITS, OP038921 and OP038922 for rpb2, OP972863 and OP972864 for endoPG, and OP972865 and OP972866 for Alt a 1, respectively). A BLASTn analysis of these sequences showed 100% identity with the reference strain CBS 117.44 of A. alternata (Fries) Keissler in the ITS (KP124303), rpb2 (KP124772), endoPG (KP124001), and Alt a 1 (KP123854) regions. Phylogenetic analyses were done using the Maximum Likelihood method based on concatenated data set and reference CBS strains using MEGA 11 software (Tamura et al., 2021). Both olive isolates clustered in the same group as A. alternata and were clearly separated from closely related Alternaria species (Figure 3), thus confirming the morphological identification and BLASTn analyses. Thirty three-year-old seedlings of olive cv. Oblica were inoculated by suspension spraying (105 conidia/ml) in three different tests and maintained in a greenhouse for 10 days at 25- 28°C with a photoperiod of 12 hr light/12 hr dark. The same number of control plants were inoculated with sterilised distilled water. Necrotic flowers and buds appeared on infected seedlings, and A. alternata was consistently reisolated (Figure 4). No symptoms appeared on the control plants. This is the first report of A. alternata causing bud and blossom blight on olive in Bosnia and Herzegovina and, to the best of our knowledge, the first report of the fungus on any plant species in the country. Lagogianni et al. (2017) have previously reported A. alternata causing blight on olive in Greece. Since Mostar and Ljubuski are the major olive-producing areas and the increasi
2020年和2021年期间,在黑塞哥维那西部Ljubuški和莫斯塔尔地区观察到橄榄树(Olea europaea)出现枯萎症状,随后出现芽和花坏死。这些症状在调查的10个果园中的2个果园和83个橄榄(cv。在受影响的果园(图1)树木,达到60%的发病率。病灶边缘的小块被涂在马铃薯葡萄糖琼脂上,在25°C下12小时的光/暗条件下孵育5天后,单一真菌持续发育。将两个分离株(FAZ HZ和KRA HZ)的10日龄单分生菌培养物置于马铃薯胡萝卜琼脂(PCA)和dryyes培养基上进行形态学表征(Andersen等,2001;西蒙斯,2007)。发育的真菌菌落为橄榄状,在PCA上培养的真菌菌落产生棕色、分枝和隔生的分生孢子,分生孢子为椭圆形或卵圆形。平均分生孢子大小为24.8±1.4 × 10.1±0.6 μm(图2)。观察到的形态学特征鉴定该真菌为Alternaria section alternata (Woudenberg et al., 2015)。利用ITS (White et al., 1990)、rpb2、endoPG和Alt a1 (Woudenberg et al., 2015)引物对两株真菌DNA进行PCR扩增。从FAZ HZ和KRA HZ分离株中产生540 bp (ITS)、904 bp (rpb2)、460 bp (endoPG)和492 bp (Alt a1)的扩增子并测序(GenBank登录号分别为ITS的OP010267和OP010268, rpb2的OP038921和OP038922, endoPG的OP972863和OP972864, Alt a1的OP972865和OP972866)。经BLASTn分析,这些序列与A. alternata (Fries) Keissler的参考菌株CBS 117.44在ITS (KP124303)、rpb2 (KP124772)、endoPG (KP124001)和Alt a1 (KP123854)区同源性100%。系统发育分析采用最大似然法,基于串联数据集和参考CBS菌株,使用MEGA 11软件(Tamura等,2021)。这两个橄榄树分离株都与A. alternata聚在同一类群中,并且明显从密切相关的Alternaria物种中分离出来(图3),从而证实了形态学鉴定和BLASTn分析。三十三岁橄榄树幼苗。采用悬浮喷洒法(105个分生孢子/ml)接种三种不同试验的Oblica,在25 ~ 28℃的温室中培养10天,光周期为12小时/12小时。用灭菌蒸馏水接种相同数量的对照植株。被感染的幼苗出现了坏死的花和芽,并且互生草不断被重新分离(图4)。对照植株没有出现任何症状。这是在波斯尼亚和黑塞哥维那首次报道引起橄榄芽和花枯萎病的A. alternata,据我们所知,也是该国首次报道这种真菌感染任何植物物种。Lagogianni等人(2017)此前报道了在希腊引起橄榄枯萎病的a.s alternata。由于Mostar和Ljubuski是主要的橄榄产区,而且橄榄在该国的重要性日益增加,这种多食性病原体可能对橄榄生产构成严重威胁,并可能蔓延到其他作物。广泛的监测和早期诊断对于这种疾病的适当管理至关重要。本研究由克罗地亚科学基金会安装研究项目资助UIP-2020-02-7413天然生物活性化合物作为控制橄榄细菌和其他真菌病原体的潜在抗菌剂来源(anti - microbi - ol)。
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引用次数: 0
First report of Avocado sunblotch viroid in avocado in Kenya 肯尼亚牛油果类日斑病病毒首次报道
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12212
P. Kibwage, E. Avedi, H. Kipkoech, E. Muthomi, M. Oronje, I. Macharia, T. Mutui
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引用次数: 0
First report of Pyrenophora avenicola causing leaf spot disease on barley 大麦叶斑病病原菌的首次报道
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12213
P. C. C. Rodrigues, P. Angelotti‐Zampar, G. Seron, H. R. Feksa, D. Tessmann
{"title":"First report of Pyrenophora avenicola causing leaf spot disease on barley","authors":"P. C. C. Rodrigues, P. Angelotti‐Zampar, G. Seron, H. R. Feksa, D. Tessmann","doi":"10.1002/ndr2.12213","DOIUrl":"https://doi.org/10.1002/ndr2.12213","url":null,"abstract":"","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48107216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First report of Snowdrop virus Y and Turnip yellows virus in Narcissus sp. in the United Kingdom Snowdrop病毒Y和萝卜黄病毒在英国水仙花属中的首次报道
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12200
S. Forde, V. Harju, A. Skelton, I. Adams, A. Fowkes, H. Pufal, S. McGreig, C. Conyers, R. Ward, L. Frew, A. Buxton-Kirk, M. Kelly, A. Fox
{"title":"First report of Snowdrop virus Y and Turnip yellows virus in Narcissus sp. in the United Kingdom","authors":"S. Forde, V. Harju, A. Skelton, I. Adams, A. Fowkes, H. Pufal, S. McGreig, C. Conyers, R. Ward, L. Frew, A. Buxton-Kirk, M. Kelly, A. Fox","doi":"10.1002/ndr2.12200","DOIUrl":"https://doi.org/10.1002/ndr2.12200","url":null,"abstract":"","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48771742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First report of a ‘Candidatus Phytoplasma asteris’‐related strain (16SrI‐B) associated with three Sempervivum cultivars in Hungary 匈牙利一株与三个Sempervivum品种相关的“Candidatus PhytopPlasma asteris”相关菌株(16SrI‐B)的首次报告
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12199
O. Viczián, J. Fodor, J. Ágoston, E. Mergenthaler
{"title":"First report of a ‘Candidatus Phytoplasma asteris’‐related strain (16SrI‐B) associated with three Sempervivum cultivars in Hungary","authors":"O. Viczián, J. Fodor, J. Ágoston, E. Mergenthaler","doi":"10.1002/ndr2.12199","DOIUrl":"https://doi.org/10.1002/ndr2.12199","url":null,"abstract":"","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48816715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fusarium acutatum is a major pathogen contributing to basal rot of onion in India 尖锐镰刀菌是印度洋葱基腐病的主要病原菌
Q3 Agricultural and Biological Sciences Pub Date : 2023-04-01 DOI: 10.1002/ndr2.12176
G. Bhat, A. Rajakumara, S. Bhangigoudra, U. Karthik, G. Shivakumar, B. B. Madalageri, P. Noojibail, R. Anandalakshmi
{"title":"Fusarium acutatum\u0000 is a major pathogen contributing to basal rot of onion in India","authors":"G. Bhat, A. Rajakumara, S. Bhangigoudra, U. Karthik, G. Shivakumar, B. B. Madalageri, P. Noojibail, R. Anandalakshmi","doi":"10.1002/ndr2.12176","DOIUrl":"https://doi.org/10.1002/ndr2.12176","url":null,"abstract":"","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42732222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
New Disease Reports
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