Pub Date : 2019-01-13DOI: 10.24931/2413-9432-2018-7-4-4-10
Т П Артемьева, Д. А. Церковский
The aim of study is to evaluate the tolerability and effectiveness of photodynamic therapy as an organ‑preserving treatment in patients with vulvar leukoplakia. 50 patients with a verifed diagnosis of «vulvar leukoplakia» were included in the study. The age varied from 27 to 74 years. The method of treatment assumed the use of the photosensitizer рhotolon (RUE «Belmedpreparaty», Belarus) administered intravenously in doses of 1.8–2.5 mg/kg. Photoirradiation of pathological foci was carried out 2.5–3 hours after intravenous injection of photolon® using a semiconductor laser «UPL PDT» (LEMT, Belarus, λ=661 nm) at exposure doses from 30 to 100 J/cm2 with a power density of 100–170 mW/cm2. The treatment was performed under medical anesthesia. The results of treatment were evaluated using clinical data. Adverse reactions and complications after the introduction of the photosensitizer and photoirradiation have not been observed. Complete clinical regression of the treated pathological foci was noted in 100% of cases with a follow‑up observation 1 month after the treatment. At follow‑up after 3 months, local recurrences of the disease were detected in 4 cases, which were successfully treated with repeated photo‑dynamic therapy sessions. The percentage of complete regressions was 92%, partial – 8%. The obtained results allow judging on the possibility of using photodynamic therapy in the treatment of patients with vulvar leukoplakia, which allows to preserve the organ and obtain a satisfactory functional and cosmetic result.
{"title":"Фотодинамическая терапия при лейкоплакии вульвы","authors":"Т П Артемьева, Д. А. Церковский","doi":"10.24931/2413-9432-2018-7-4-4-10","DOIUrl":"https://doi.org/10.24931/2413-9432-2018-7-4-4-10","url":null,"abstract":"The aim of study is to evaluate the tolerability and effectiveness of photodynamic therapy as an organ‑preserving treatment in patients with vulvar leukoplakia. 50 patients with a verifed diagnosis of «vulvar leukoplakia» were included in the study. The age varied from 27 to 74 years. The method of treatment assumed the use of the photosensitizer рhotolon (RUE «Belmedpreparaty», Belarus) administered intravenously in doses of 1.8–2.5 mg/kg. Photoirradiation of pathological foci was carried out 2.5–3 hours after intravenous injection of photolon® using a semiconductor laser «UPL PDT» (LEMT, Belarus, λ=661 nm) at exposure doses from 30 to 100 J/cm2 with a power density of 100–170 mW/cm2. The treatment was performed under medical anesthesia. The results of treatment were evaluated using clinical data. Adverse reactions and complications after the introduction of the photosensitizer and photoirradiation have not been observed. Complete clinical regression of the treated pathological foci was noted in 100% of cases with a follow‑up observation 1 month after the treatment. At follow‑up after 3 months, local recurrences of the disease were detected in 4 cases, which were successfully treated with repeated photo‑dynamic therapy sessions. The percentage of complete regressions was 92%, partial – 8%. The obtained results allow judging on the possibility of using photodynamic therapy in the treatment of patients with vulvar leukoplakia, which allows to preserve the organ and obtain a satisfactory functional and cosmetic result.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"7 1","pages":"4-10"},"PeriodicalIF":0.0,"publicationDate":"2019-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42792206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.24931/2413-9432-2019-8-2-25-30
I. Reshetov, A. Fatyanova, Yu. V. Babaeva, M. M. Gafarov, K. V. Ogdanskaya, T. E. Suhova, S. Korenev, M. V. Denisenko, Y. Romanko
Currently, photodynamic therapy (PDT) remains the most effective treatment for actinic keratosis (AK). With the increase in the incidence of AK, mainly due to the popularization of recreation in countries with increased insolation, there is an increasing interest in developing new methods of diagnostics and treatment and improving the existing ones. Studies that are aimed at determining the final efficacy of PDT, taking into account the resulting adverse reactions and long-term cosmetic results, are becoming increasingly popular. The nature of the light needed to excite a photosensitizer (PS) opens up new possibilities in the field of experimental studies that are aimed at reducing adverse reactions with similar efficacy of the applied therapy. In the review article, we presented the results of our own and foreign studies on the diagnosis and treatment of AK for 2017–2019, namely: we determined the possibilities of using sources with natural and short-wave radiation at different depths of skin lesions; presented a classification of the growth of AK in the basal layer of the epidermis, which increases the possibility of predicting the outcomes of the disease; showed the prevailing efficiency of fluorescent diagnostics compared with traditional diagnostic methods; evaluated the advantages of PDT using natural light and artificial sources of radiation; described the possibility of using a combination of drugs to increase the effectiveness of PDT in difficult to treat areas and in AK foci with a high degree of damage to the basal layer of the epidermis.
{"title":"Modern aspects of photodynamic therapy of actinic keratoses","authors":"I. Reshetov, A. Fatyanova, Yu. V. Babaeva, M. M. Gafarov, K. V. Ogdanskaya, T. E. Suhova, S. Korenev, M. V. Denisenko, Y. Romanko","doi":"10.24931/2413-9432-2019-8-2-25-30","DOIUrl":"https://doi.org/10.24931/2413-9432-2019-8-2-25-30","url":null,"abstract":"Currently, photodynamic therapy (PDT) remains the most effective treatment for actinic keratosis (AK). With the increase in the incidence of AK, mainly due to the popularization of recreation in countries with increased insolation, there is an increasing interest in developing new methods of diagnostics and treatment and improving the existing ones. Studies that are aimed at determining the final efficacy of PDT, taking into account the resulting adverse reactions and long-term cosmetic results, are becoming increasingly popular. The nature of the light needed to excite a photosensitizer (PS) opens up new possibilities in the field of experimental studies that are aimed at reducing adverse reactions with similar efficacy of the applied therapy. In the review article, we presented the results of our own and foreign studies on the diagnosis and treatment of AK for 2017–2019, namely: we determined the possibilities of using sources with natural and short-wave radiation at different depths of skin lesions; presented a classification of the growth of AK in the basal layer of the epidermis, which increases the possibility of predicting the outcomes of the disease; showed the prevailing efficiency of fluorescent diagnostics compared with traditional diagnostic methods; evaluated the advantages of PDT using natural light and artificial sources of radiation; described the possibility of using a combination of drugs to increase the effectiveness of PDT in difficult to treat areas and in AK foci with a high degree of damage to the basal layer of the epidermis.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68974396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-18DOI: 10.24931/2413-9432-2018-7-3-47-56
S. Petrov, R. P. Poleva
Selective laser trabeculoplasty (SLT) is the “golden standard” of laser glaucoma surgery. Its efficacy can be compared to pharmacological therapy, while in some cases its advantages may even lead to a more stable hypotensive effect. SLT may be used as a primary treatment for primary open-angle glaucoma patients and patients with ocular hypertension, while also considered safe and effective in cases when a repeat procedure is required. SLT may potentially decrease the demand for antiglaucoma drugs, improve patient’s response to treatment, make the treatment more comfortable and overall increase the patient’s quality of life. New modifications of standard laser treatment procedures have been emerging lately. This article summarizes scientific data on the efficacy and safety of the new generation of laser trabeculoplasty. It specifies the charateristics of micropulse laser trabeculoplasty (MLT), pattern-scanning trabeculoplasty (PLT) and titaniumsapphire laser trabeculoplasty (TSLT) and recounts the latest research dedicated to them.
{"title":"New approaches to selective laser trabeculoplasty","authors":"S. Petrov, R. P. Poleva","doi":"10.24931/2413-9432-2018-7-3-47-56","DOIUrl":"https://doi.org/10.24931/2413-9432-2018-7-3-47-56","url":null,"abstract":"Selective laser trabeculoplasty (SLT) is the “golden standard” of laser glaucoma surgery. Its efficacy can be compared to pharmacological therapy, while in some cases its advantages may even lead to a more stable hypotensive effect. SLT may be used as a primary treatment for primary open-angle glaucoma patients and patients with ocular hypertension, while also considered safe and effective in cases when a repeat procedure is required. SLT may potentially decrease the demand for antiglaucoma drugs, improve patient’s response to treatment, make the treatment more comfortable and overall increase the patient’s quality of life. New modifications of standard laser treatment procedures have been emerging lately. This article summarizes scientific data on the efficacy and safety of the new generation of laser trabeculoplasty. It specifies the charateristics of micropulse laser trabeculoplasty (MLT), pattern-scanning trabeculoplasty (PLT) and titaniumsapphire laser trabeculoplasty (TSLT) and recounts the latest research dedicated to them.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68973865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-18DOI: 10.24931/2413-9432-2018-7-3-21-28
L. A. Vasilev, N. Panov, V. Kapinus, M. Kaplan, I. Kostyuk, A. Kaprin
This work presents the results of performing intraoperative photodynamic therapy (IOPDT) on 22 patients with recurrent pelvic tumors(cervical cancer – in 18 patients, cancer of the corpus uteri – in 3 patients, cancer of the anal canal – in 1 patient). Prior to the PDT procedure, the patients were injected with photolon photosensitizer (PS) at a dose of 1.0–1.1 mg/kg. After the injection of PS, local fluorescence spectroscopy of tumor lesions was performed to determine the accumulation of drug in various areas of tumors and healthy tissue. Intraoperative laser irradiation was carried out 3–5 hours after the photolon injection with light at 662 nm wavelength using "Latus-2" laser device with a power density of 140 mW/cm2and the density of light energy of 40–60 J/cm2, the number of irradiation fields was 3–5 depending on the anatomical features.The follow-up period after surgical treatment combined with PDT was from 6 to 24 months. Analyzing the immediate results of the treatment, there were no undesirable events or increase in the number of postoperative complications compared to patients treated without IOPDT. Were registered: transient increase in ALT and AST levels – in 5 patients (13.6%), reduction of oxygenation during anesthesia – in 20 (90.9%), transient fevers in the postoperative period – in 7 (31.8%).It was noted that IOPDT with photolon drug, while slightly extending the time of the operation, is well tolerated by patients and does not lead to an increase in the number of early postoperative complications or the length of hospitalization.
{"title":"Intraoperative fluorescent spectroscopy and photodynamic therapy of recurrent pelvis minor tumors with local radiation damage","authors":"L. A. Vasilev, N. Panov, V. Kapinus, M. Kaplan, I. Kostyuk, A. Kaprin","doi":"10.24931/2413-9432-2018-7-3-21-28","DOIUrl":"https://doi.org/10.24931/2413-9432-2018-7-3-21-28","url":null,"abstract":"This work presents the results of performing intraoperative photodynamic therapy (IOPDT) on 22 patients with recurrent pelvic tumors(cervical cancer – in 18 patients, cancer of the corpus uteri – in 3 patients, cancer of the anal canal – in 1 patient). Prior to the PDT procedure, the patients were injected with photolon photosensitizer (PS) at a dose of 1.0–1.1 mg/kg. After the injection of PS, local fluorescence spectroscopy of tumor lesions was performed to determine the accumulation of drug in various areas of tumors and healthy tissue. Intraoperative laser irradiation was carried out 3–5 hours after the photolon injection with light at 662 nm wavelength using \"Latus-2\" laser device with a power density of 140 mW/cm2and the density of light energy of 40–60 J/cm2, the number of irradiation fields was 3–5 depending on the anatomical features.The follow-up period after surgical treatment combined with PDT was from 6 to 24 months. Analyzing the immediate results of the treatment, there were no undesirable events or increase in the number of postoperative complications compared to patients treated without IOPDT. Were registered: transient increase in ALT and AST levels – in 5 patients (13.6%), reduction of oxygenation during anesthesia – in 20 (90.9%), transient fevers in the postoperative period – in 7 (31.8%).It was noted that IOPDT with photolon drug, while slightly extending the time of the operation, is well tolerated by patients and does not lead to an increase in the number of early postoperative complications or the length of hospitalization.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68974087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-18DOI: 10.24931/2413-9432-2018-7-3-43-46
R. Yakubovskaya, A. Pankratov, E. Filonenko, E. Lukyanets, V. Ivanova-Radkevich, A. Trushin, A. Kaprin
A comparative experimental study of the specific activity of drugs based on 5-aminolevulinic acid (5-ALA) and its hexyl ester (5-ALA HE) was carried out. Their ability to induce the synthesis of photoactive protoporphyrin IX in the healthy tissues of the rabbit bladder when instilling the drug solutions at various concentrations has been estimated. It was shown that 5-ALA HE results in the induction and accumulation of PPIX in the rabbit bladder epithelium at much lower concentrations than 5-ALA. Thus, a significant increase in the fluorescence intensity in comparison with the control was achieved by instillation of 5-ALA HE solution in the rabbit’ bladder at a concentration of only 0.0001% (fluorescence intensity 2.20±0.60 a.u.), and for 5-ALA – only when using a solution at a concentration of 0.3% (fluorescence intensity 2.60±1.02 a.u.).
{"title":"Comparative experimental study of 5-ALA and 5-ALA hexyl ester specific activity","authors":"R. Yakubovskaya, A. Pankratov, E. Filonenko, E. Lukyanets, V. Ivanova-Radkevich, A. Trushin, A. Kaprin","doi":"10.24931/2413-9432-2018-7-3-43-46","DOIUrl":"https://doi.org/10.24931/2413-9432-2018-7-3-43-46","url":null,"abstract":"A comparative experimental study of the specific activity of drugs based on 5-aminolevulinic acid (5-ALA) and its hexyl ester (5-ALA HE) was carried out. Their ability to induce the synthesis of photoactive protoporphyrin IX in the healthy tissues of the rabbit bladder when instilling the drug solutions at various concentrations has been estimated. It was shown that 5-ALA HE results in the induction and accumulation of PPIX in the rabbit bladder epithelium at much lower concentrations than 5-ALA. Thus, a significant increase in the fluorescence intensity in comparison with the control was achieved by instillation of 5-ALA HE solution in the rabbit’ bladder at a concentration of only 0.0001% (fluorescence intensity 2.20±0.60 a.u.), and for 5-ALA – only when using a solution at a concentration of 0.3% (fluorescence intensity 2.60±1.02 a.u.).","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44989703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-18DOI: 10.24931/2413-9432-2018-7-3-29-35
A. Zikiryahodjaev, M. Ermoshchenkova, V. Chissov, I. M. Shirokih
Currently, reconstructive-plastic surgery is becoming the standard for surgical and combined treatment of breast cancer (BC) patients.With a one-stage reconstruction of the breast, the use of biological implants that can replace muscle autografts and, consequently, reduce trauma, blood loss and operation time, and also to avoid defects in the donor zones is becoming topical. From 2014 to 2017, 151 reconstructive operations on 121 patients with BC (average age 41.5 years) using synthetic and biological materials were carried out in P.Herzen Moscow Oncology Research Institute. 0 stage of BC TisN0M0 was diagnosed in 11 (9.1%) patients, I stage – in 52 (42.9%), IIA – in 41 (33.9%), IIB – in 9 (7.4%), IIIA – in 4 (3.3%), IIIB – in 2 (1.7%), IIIC – in 2 (1.7%). To strengthen the lower slope, a biological implant – Permacol acellular dermal matrix, was used in 34 cases. Cosmetic result was rated as excellent in 20 (58.9%) cases, good in 11 (32.3%), unsatisfactory in 3 (8,8%). Biological materials are an important addition to various options for breast reconstruction. The criterion for selecting the material for strengthening the lower slope of the breast with subcutaneous or cutaneous mastectomy for cancer with a one-stage reconstruction using a silicone endoprosthesis is the pinch-test value.
{"title":"Biological materials in breast cancer reconstructive surgery","authors":"A. Zikiryahodjaev, M. Ermoshchenkova, V. Chissov, I. M. Shirokih","doi":"10.24931/2413-9432-2018-7-3-29-35","DOIUrl":"https://doi.org/10.24931/2413-9432-2018-7-3-29-35","url":null,"abstract":"Currently, reconstructive-plastic surgery is becoming the standard for surgical and combined treatment of breast cancer (BC) patients.With a one-stage reconstruction of the breast, the use of biological implants that can replace muscle autografts and, consequently, reduce trauma, blood loss and operation time, and also to avoid defects in the donor zones is becoming topical. From 2014 to 2017, 151 reconstructive operations on 121 patients with BC (average age 41.5 years) using synthetic and biological materials were carried out in P.Herzen Moscow Oncology Research Institute. 0 stage of BC TisN0M0 was diagnosed in 11 (9.1%) patients, I stage – in 52 (42.9%), IIA – in 41 (33.9%), IIB – in 9 (7.4%), IIIA – in 4 (3.3%), IIIB – in 2 (1.7%), IIIC – in 2 (1.7%). To strengthen the lower slope, a biological implant – Permacol acellular dermal matrix, was used in 34 cases. Cosmetic result was rated as excellent in 20 (58.9%) cases, good in 11 (32.3%), unsatisfactory in 3 (8,8%). Biological materials are an important addition to various options for breast reconstruction. The criterion for selecting the material for strengthening the lower slope of the breast with subcutaneous or cutaneous mastectomy for cancer with a one-stage reconstruction using a silicone endoprosthesis is the pinch-test value.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68973740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-10DOI: 10.24931/2413-9432-2018-7-3-36-42
Д. А. Кравчук
Malaria causes a serious health problem in the tropical and subtropical regions of the globe. In many cases, the consequences of this disease are fatal. Therefore, a simple, fast, accurate and affordable diagnostic system for the early detection of this disease is necessary for the timely administration of antimalarial drugs. The malarial parasite, during its intra-erythrocyte development, processes a significant amount of hemoglobin, which in this case turns into a hem form called hemozoin. Hemozoin and hemoglobin have different molar extinction coefficients at certain optical wavelengths, hence, light absorption and an optoacoustic signal (OAS) from the infected cell will be different from that of a healthy cell. The paper describes the developed theoretical model intended for studying the influence of intra-erythrocyte malarial parasite development on optoacoustic signals. The OAS were calculated based on the models of healthy and infected blood modeled on the basis of a 3D model. The simulated OAS were analyzed in the temporal and frequency domains to obtain signs of infection at various stages. The calculated OAS spectra have different amplitude levels, which indicates that the optoacoustic method can be useful for differentiating various intraerythrocyte stages of the malarial parasite. The carried out modeling and the results obtained allow us to continue working on the creation of an optoacoustic flow cytometer.
{"title":"Математическая модель обнаружения внутриэритроцитарных инфекций с помощью оптоакустического метода","authors":"Д. А. Кравчук","doi":"10.24931/2413-9432-2018-7-3-36-42","DOIUrl":"https://doi.org/10.24931/2413-9432-2018-7-3-36-42","url":null,"abstract":"Malaria causes a serious health problem in the tropical and subtropical regions of the globe. In many cases, the consequences of this disease are fatal. Therefore, a simple, fast, accurate and affordable diagnostic system for the early detection of this disease is necessary for the timely administration of antimalarial drugs. The malarial parasite, during its intra-erythrocyte development, processes a significant amount of hemoglobin, which in this case turns into a hem form called hemozoin. Hemozoin and hemoglobin have different molar extinction coefficients at certain optical wavelengths, hence, light absorption and an optoacoustic signal (OAS) from the infected cell will be different from that of a healthy cell. The paper describes the developed theoretical model intended for studying the influence of intra-erythrocyte malarial parasite development on optoacoustic signals. The OAS were calculated based on the models of healthy and infected blood modeled on the basis of a 3D model. The simulated OAS were analyzed in the temporal and frequency domains to obtain signs of infection at various stages. The calculated OAS spectra have different amplitude levels, which indicates that the optoacoustic method can be useful for differentiating various intraerythrocyte stages of the malarial parasite. The carried out modeling and the results obtained allow us to continue working on the creation of an optoacoustic flow cytometer.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"7 1","pages":"36-42"},"PeriodicalIF":0.0,"publicationDate":"2018-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68973789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-07-05DOI: 10.18008/1816-5095-2018-2-176-181
S. Trufanov, A. Fedorov, V. R. Mamikonyan, L. Tekeeva, S. A. Malozhen, A. Karamyan
Purpose: to develop the optimal (safe and effective) method of diamond burr polishing of Bowman’s membrane (DBPBM) for the possible use it in the treatment of the recurrent corneal erosion syndrome (RCES) in the experiment.Material and methods. DBPBM in different modifications of the method and the mechanical deepithelization were performed in experiment on 19 human cadaver eyes. We use system Ophtho-Burr for DBPBM that includes a tip and ophthalmic burrs with diameter 1mm, 2.5 mm, 5 mm, varying the speed of rotation of burr. For 3 eyes — polishing with the application of burr with a diameter of 5 mm, the rotation frequency of 4000 rpm using 2 passes over the surface of the cornea. For 3 eyes — burr with a diameter of 2.5 mm, rotation frequency of 4000 rpm in 2 perpendicular passes. For 3 eyes — diameter burr 1 mm at speed of 8000 rpm in 2 perpendicular passes. For 3 eyes — diameter burr 1 mm at speed 4000 rpm with 4 passes along the surface of the cornea. For 4 eyes — diameter was 1 mm at speed of 4000 rpm in 2 perpendicular passes. For 3 the eyes was conducted mechanical deepithelization. Subsequent histopathological investigation of the cornea was performed by method of semi-thin section, with polychrome staining.Results. The obtained results of histological studies of DBPBM in different versions on cadaver cornea is allowed to choose the optimal variant of surgical intervention for use in clinical conditions. It consisted in a selection of burr with a diameter of 1 mm, the rotation frequency of the 4000 rpm and 2 perpendicular passes with a moderate compression of the cornea. The abovementioned technique of intervention allows removing completely the corneal epithelium with a basal membrane and save uniform in thickness, fully deepitelizationed Bowman’s membrane.Conclusion.Careful and accurate removal of dysplastic corneal epithelium in cases of the recurrent corneal erosion syndrome using proposed method in clinical conditions should contribute to the creation optimal environments for adequate reepithelization with steady epithelial-stromal adhesion. The remaining nearly intact Bowman’s membrane after procedure can prevent some postoperative complications such as haze and induced refractive disorders.
目的:探索鲍曼膜金刚石毛刺抛光(DBPBM)的最佳(安全有效)方法，以期在实验中用于治疗复发性角膜侵蚀综合征(RCES)。材料和方法。在19只人的尸体眼睛上进行了不同修改方法的DBPBM和机械深度化实验。我们使用系统眼科毛刺DBPBM，包括一个尖端和直径1mm, 2.5 mm, 5mm的眼科毛刺，改变毛刺的旋转速度。对3只眼睛进行抛光，使用直径为5mm的毛刺，旋转频率为4000转/分，在角膜表面使用2次。对于直径为2.5毫米的3眼毛刺，在2个垂直的通道中旋转频率为4000 rpm。对于3眼直径毛刺1毫米在速度8000转/分钟在2个垂直通道。以4000转/分钟的速度沿角膜表面进行4次毛刺，3眼直径毛刺1毫米。4眼直径为1毫米，速度为4000转/分钟，在2个垂直通道。对3只眼进行机械深度摘除术。采用半薄切片法对角膜进行组织病理学检查，并进行彩色染色。不同版本的DBPBM在尸体角膜上的组织学研究结果，允许选择最佳的手术干预变体用于临床条件。它包括选择直径为1mm的毛刺，旋转频率为4000 rpm，垂直通过2次，适度压缩角膜。结论:在临床条件下，在复发性角膜侵蚀综合征病例中，采用上述干预技术，仔细准确地去除发育不良的角膜上皮，有助于为充分的再上皮形成和稳定的上皮-间质粘连创造最佳环境。术后保留的几乎完整的鲍曼膜可以防止一些术后并发症，如雾霭和诱发的屈光不正。
{"title":"Experimental Study of Diamond Burr Polishing Methods of Bowman’s Membrane","authors":"S. Trufanov, A. Fedorov, V. R. Mamikonyan, L. Tekeeva, S. A. Malozhen, A. Karamyan","doi":"10.18008/1816-5095-2018-2-176-181","DOIUrl":"https://doi.org/10.18008/1816-5095-2018-2-176-181","url":null,"abstract":"Purpose: to develop the optimal (safe and effective) method of diamond burr polishing of Bowman’s membrane (DBPBM) for the possible use it in the treatment of the recurrent corneal erosion syndrome (RCES) in the experiment.Material and methods. DBPBM in different modifications of the method and the mechanical deepithelization were performed in experiment on 19 human cadaver eyes. We use system Ophtho-Burr for DBPBM that includes a tip and ophthalmic burrs with diameter 1mm, 2.5 mm, 5 mm, varying the speed of rotation of burr. For 3 eyes — polishing with the application of burr with a diameter of 5 mm, the rotation frequency of 4000 rpm using 2 passes over the surface of the cornea. For 3 eyes — burr with a diameter of 2.5 mm, rotation frequency of 4000 rpm in 2 perpendicular passes. For 3 eyes — diameter burr 1 mm at speed of 8000 rpm in 2 perpendicular passes. For 3 eyes — diameter burr 1 mm at speed 4000 rpm with 4 passes along the surface of the cornea. For 4 eyes — diameter was 1 mm at speed of 4000 rpm in 2 perpendicular passes. For 3 the eyes was conducted mechanical deepithelization. Subsequent histopathological investigation of the cornea was performed by method of semi-thin section, with polychrome staining.Results. The obtained results of histological studies of DBPBM in different versions on cadaver cornea is allowed to choose the optimal variant of surgical intervention for use in clinical conditions. It consisted in a selection of burr with a diameter of 1 mm, the rotation frequency of the 4000 rpm and 2 perpendicular passes with a moderate compression of the cornea. The abovementioned technique of intervention allows removing completely the corneal epithelium with a basal membrane and save uniform in thickness, fully deepitelizationed Bowman’s membrane.Conclusion.Careful and accurate removal of dysplastic corneal epithelium in cases of the recurrent corneal erosion syndrome using proposed method in clinical conditions should contribute to the creation optimal environments for adequate reepithelization with steady epithelial-stromal adhesion. The remaining nearly intact Bowman’s membrane after procedure can prevent some postoperative complications such as haze and induced refractive disorders.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67552133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-25DOI: 10.24931/2413-9432-2018-7-2-25-36
I. Romanishkin, D. Pominova, P. Grachev, V. Makarov, A. Vanetsev, E. Orlovskaya, A. Baranchikov, I. Sildos, V. Loschenov, Y. Orlovskii, A. Ryabova
Laser hyperthermia is one of the promising methods for treatment of oncological diseases. For routine clinical use of hyperthermia, it is necessary to control the uniformity and localization of heat within the tumor. Local heating can be achieved by using special thermal agents, such as nanoparticles doped with rare-earth ions. Measurement of the temperature of the thermal agents will allow timely regulation of the applied laser radiation excitation power and optimization of the hyperthermia process.The paper presents the results of a study on the non-invasive determination of the YPO4 nanoparticles doped with Nd3+ temperature with sensitivity of 0.2% °С-1 in 30-60°С temperature range. The temperature of the nanoparticles was calculated from the Nd3+ luminescence spectra in the 800-1000 nm range under excitation into 4F5/2 energy state by 805 nm laser. A calibration procedure for recalculating the ratio of the luminescence intensities from the Stark sublevels of the 4F3/2 Nd3+ state into the values of the real NP temperature in accordance with the Boltzmann distribution is given. An algorithm for calculating luminescence intensities for individual Stark components is proposed. After calculating the intensities corresponding to each individual Stark component, all the intensities related to the transition from the upper and lower Stark sublevels of the 4F3/2 state are summed, and then their ratio is calculated. The resulting ratio is normalized to the value of the ratio at room temperature and, in accordance with the calibration dependence, is recalculated into the NP heating temperature. It was demonstrated that the investigated 1%Nd3+:YPO4 nanoparticles can be used as "primary” thermometers that do not require additional recalibration to evaluate the temperature in the range used for hyperthermia.
{"title":"NONINVASIVE ESTIMATION OF THE LOCAL TEMPERATURE OF BIOTISSUES HEATING UNDER THE ACTION OF LASER IRRADIATION FROM THE LUMINESCENCE SPECTRA OF Nd3+ IONS","authors":"I. Romanishkin, D. Pominova, P. Grachev, V. Makarov, A. Vanetsev, E. Orlovskaya, A. Baranchikov, I. Sildos, V. Loschenov, Y. Orlovskii, A. Ryabova","doi":"10.24931/2413-9432-2018-7-2-25-36","DOIUrl":"https://doi.org/10.24931/2413-9432-2018-7-2-25-36","url":null,"abstract":"Laser hyperthermia is one of the promising methods for treatment of oncological diseases. For routine clinical use of hyperthermia, it is necessary to control the uniformity and localization of heat within the tumor. Local heating can be achieved by using special thermal agents, such as nanoparticles doped with rare-earth ions. Measurement of the temperature of the thermal agents will allow timely regulation of the applied laser radiation excitation power and optimization of the hyperthermia process.The paper presents the results of a study on the non-invasive determination of the YPO4 nanoparticles doped with Nd3+ temperature with sensitivity of 0.2% °С-1 in 30-60°С temperature range. The temperature of the nanoparticles was calculated from the Nd3+ luminescence spectra in the 800-1000 nm range under excitation into 4F5/2 energy state by 805 nm laser. A calibration procedure for recalculating the ratio of the luminescence intensities from the Stark sublevels of the 4F3/2 Nd3+ state into the values of the real NP temperature in accordance with the Boltzmann distribution is given. An algorithm for calculating luminescence intensities for individual Stark components is proposed. After calculating the intensities corresponding to each individual Stark component, all the intensities related to the transition from the upper and lower Stark sublevels of the 4F3/2 state are summed, and then their ratio is calculated. The resulting ratio is normalized to the value of the ratio at room temperature and, in accordance with the calibration dependence, is recalculated into the NP heating temperature. It was demonstrated that the investigated 1%Nd3+:YPO4 nanoparticles can be used as \"primary” thermometers that do not require additional recalibration to evaluate the temperature in the range used for hyperthermia.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41347915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-25DOI: 10.24931/2413-9432-2018-7-2-4-12
M. Kholodtsova, P. Grachev, W. Blondel, P. Zelenkov, Alexander A. Potapov, I. Shcherbakov, V. Loschenov
The abilities of the optical probe for fluorescence-guided stereotactic biopsy were investigated by means of a multifiber probe under 532 or 632.8 nm excitation. The set of multilayered phantoms representing a border between normal brain-tissue and a tumor with photosensitizer and gold nanoparticles (spheres or stars) in different concentrations were made to investigate the macroscopic parameters observed during the neurosurgical biopsy sample collection by means of the optical probe. These investigantions will allow to define the border of a brain tumor and control the type of tissue being collected by a stereotactic cannula during surgery. The impact of gold nanospheres and gold nanostars added in different concentrations to the bottom-layer of phantom was analyzed by fluorescence quenching or enhancement due to energy transfer between nanoparticles and fluorescent molecules. The results allow determining the tumor border and to make the biopsy uptake more efficient by observing the character of signal change while penetrating a tumor and while going out of it.
{"title":"APPLICATION OF DEVICES FOR SPACE-RESOLVED SPECTROSCOPY ON THE EXAMPLE OF TWO-LAYER PHANTOMS CONTAINING METALLIC NANOPARTICLES","authors":"M. Kholodtsova, P. Grachev, W. Blondel, P. Zelenkov, Alexander A. Potapov, I. Shcherbakov, V. Loschenov","doi":"10.24931/2413-9432-2018-7-2-4-12","DOIUrl":"https://doi.org/10.24931/2413-9432-2018-7-2-4-12","url":null,"abstract":"The abilities of the optical probe for fluorescence-guided stereotactic biopsy were investigated by means of a multifiber probe under 532 or \u0000632.8 nm excitation. The set of multilayered phantoms representing a border between normal brain-tissue and a tumor with photosensitizer \u0000and gold nanoparticles (spheres or stars) in different concentrations were made to investigate the macroscopic parameters observed during the \u0000neurosurgical biopsy sample collection by means of the optical probe. These investigantions will allow to define the border of a brain tumor and \u0000control the type of tissue being collected by a stereotactic cannula during surgery. The impact of gold nanospheres and gold nanostars added \u0000in different concentrations to the bottom-layer of phantom was analyzed by fluorescence quenching or enhancement due to energy transfer \u0000between nanoparticles and fluorescent molecules. The results allow determining the tumor border and to make the biopsy uptake more efficient \u0000by observing the character of signal change while penetrating a tumor and while going out of it.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49146893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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