Pub Date : 2019-07-11DOI: 10.24931/2413-9432-2012-1-1-3-5
В. А. Титова
The application of photodynamic therapy combined with local laser hyperthermia in patients with exhausted surgical treatment, radiotherapy and other cancer therapy is reviewed. Pre-requisites and relevancy of concurrent use of these methods in different groups of patients. The experience for this concurrent treatment in 60 patients with malignant tumors of oral, vulvar, rectal and other mucosa is summarized. Indications and eligibility criteria are represented, the efficiency and safety of developed methods are illustrated by case-reports.
{"title":"Роль и место фотодинамической терапии в мультимодальных программах лечения злокачественных опухолей","authors":"В. А. Титова","doi":"10.24931/2413-9432-2012-1-1-3-5","DOIUrl":"https://doi.org/10.24931/2413-9432-2012-1-1-3-5","url":null,"abstract":"The application of photodynamic therapy combined with local laser hyperthermia in patients with exhausted surgical treatment, radiotherapy and other cancer therapy is reviewed. Pre-requisites and relevancy of concurrent use of these methods in different groups of patients. The experience for this concurrent treatment in 60 patients with malignant tumors of oral, vulvar, rectal and other mucosa is summarized. Indications and eligibility criteria are represented, the efficiency and safety of developed methods are illustrated by case-reports.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"1 1","pages":"3-5"},"PeriodicalIF":0.0,"publicationDate":"2019-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46481992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-10DOI: 10.24931/2413-9432-2019-8-2-14-18
Г. П. Попова, Я. А. Накатис, М. А. Рымша
Inflammatory diseases of the sinuses – one of the most common nosologies in the practice of otorhinolaryngologist; its pathogenesis is well studied, and treatment recommendations are detailed. Following them, however, cannot completely prevent chronic disease or recurrence. Antimicrobial photodynamic therapy is a promising method of treating sinusitis, which has proved its effectiveness, but has not yet been widely used. This work describes our experience in photodynamic therapy with chlorin e6 of chronic sinusitis using a new laser diode-based irradiation source. For patients who had previous sinus surgery an adapter for penetration into the sinus through anastomosis was developed and tested. First group of the patients underwent photodynamic therapy (PDT) according to the conventional scheme, using a laser with a wavelength of 662 nm; while the second one underwent PDT using a 405 nm laser. With daily washing of the nasal sinus, the period of inflammation relief (evaluated by the absence of pathological discharge during washing) amounted to 3.8 full days on average in the first group of patients, compared to 5.4 days on average for the second group. We carried out the comparative analysis of the treatment results based on clinical assessment and radiological evaluation (CT) at the time of discharge from the hospital and 1 month later.
{"title":"Сравнение эффективности фотодинамической терапии при использовании источников излучения с различными длинами волн в лечении синуситов","authors":"Г. П. Попова, Я. А. Накатис, М. А. Рымша","doi":"10.24931/2413-9432-2019-8-2-14-18","DOIUrl":"https://doi.org/10.24931/2413-9432-2019-8-2-14-18","url":null,"abstract":"Inflammatory diseases of the sinuses – one of the most common nosologies in the practice of otorhinolaryngologist; its pathogenesis is well studied, and treatment recommendations are detailed. Following them, however, cannot completely prevent chronic disease or recurrence. Antimicrobial photodynamic therapy is a promising method of treating sinusitis, which has proved its effectiveness, but has not yet been widely used. This work describes our experience in photodynamic therapy with chlorin e6 of chronic sinusitis using a new laser diode-based irradiation source. For patients who had previous sinus surgery an adapter for penetration into the sinus through anastomosis was developed and tested. First group of the patients underwent photodynamic therapy (PDT) according to the conventional scheme, using a laser with a wavelength of 662 nm; while the second one underwent PDT using a 405 nm laser. With daily washing of the nasal sinus, the period of inflammation relief (evaluated by the absence of pathological discharge during washing) amounted to 3.8 full days on average in the first group of patients, compared to 5.4 days on average for the second group. We carried out the comparative analysis of the treatment results based on clinical assessment and radiological evaluation (CT) at the time of discharge from the hospital and 1 month later.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"8 1","pages":"14-18"},"PeriodicalIF":0.0,"publicationDate":"2019-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48004494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-10DOI: 10.24931/2413-9432-2019-8-2-19-24
A. N. Tumanina, A. Polezhaev, V. Apanasevich, L. Gurina, M. Volkov, A. Y. Tarasenko, E. Filonenko
Photodynamic therapy (PDT) is a worthy alternative to surgical esophageal resection or endoscopic mucosal resection and dissection (EMR, ESD) in patients with superficial esophageal cancer with severe concomitant diseases as well as in patients with a common form of esophageal cancer with severe malignant dysphagia. Patients with superficial (7) and advanced (15) esophageal cancer received PDT as an independent method and as a supplement to complex treatment. Radachlorin was used as a photosensitizer at a dose of 0.6–0.8 mg/kg, administered intravenously 3 hours before irradiation. A PDT session was carried out using a laser with a wavelength of 662 nm. The light dose used was 150–300 J/cm2 . The use of PDT made it possible to achieve the full effect in 7 (100%) patients in the group of superficial (T1a-T1b) esophageal cancer where PDT was either the only method of treatment or in combination with radiation therapy. In the group of patients with stenotic cancer the use of PDT made it possible to achieve full recovery of food intake after recanalization for 20% of patients, and partial – for 66.7%. Thus, complete natural food intake was restored for 86.7% of patients which improved their quality of life. PDT is also a method of choice for cancer of the upper esophagus as esophageal stenting in this situation can cause unwanted subjective sensations.
{"title":"Experience of using photodynamic therapy in the treatment of esophageal cancer","authors":"A. N. Tumanina, A. Polezhaev, V. Apanasevich, L. Gurina, M. Volkov, A. Y. Tarasenko, E. Filonenko","doi":"10.24931/2413-9432-2019-8-2-19-24","DOIUrl":"https://doi.org/10.24931/2413-9432-2019-8-2-19-24","url":null,"abstract":"Photodynamic therapy (PDT) is a worthy alternative to surgical esophageal resection or endoscopic mucosal resection and dissection (EMR, ESD) in patients with superficial esophageal cancer with severe concomitant diseases as well as in patients with a common form of esophageal cancer with severe malignant dysphagia. Patients with superficial (7) and advanced (15) esophageal cancer received PDT as an independent method and as a supplement to complex treatment. Radachlorin was used as a photosensitizer at a dose of 0.6–0.8 mg/kg, administered intravenously 3 hours before irradiation. A PDT session was carried out using a laser with a wavelength of 662 nm. The light dose used was 150–300 J/cm2 . The use of PDT made it possible to achieve the full effect in 7 (100%) patients in the group of superficial (T1a-T1b) esophageal cancer where PDT was either the only method of treatment or in combination with radiation therapy. In the group of patients with stenotic cancer the use of PDT made it possible to achieve full recovery of food intake after recanalization for 20% of patients, and partial – for 66.7%. Thus, complete natural food intake was restored for 86.7% of patients which improved their quality of life. PDT is also a method of choice for cancer of the upper esophagus as esophageal stenting in this situation can cause unwanted subjective sensations. ","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68973897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-31DOI: 10.24931/2413-9432-2019-8-1-29-37
A. Kaprin, A. Trushin, M. P. Golovachenko, V. Ivanova-Radkevich, V. Chissov, E. Filonenko
This article presents the results of a clinical study that examined the diagnostic efficacy of fluorescent diagnostics (FD) of non-muscularinvasive bladder cancer using a photosensitizer of FD of malignant neoplasms – 5-aminolevulinic acid hexyl ester (5-ALA HE) compared with standard cystoscopy. The study involved 110 patients. The study began with intravesical administration of 50 ml of 0.2% solution of 5-ALA HE, the exposure time was 1 hour, after which the drug was removed from the organ. During the next hour, the mucous membranes were examined in two cystoscopy modes, followed by a standard transurethral resection of all urothelium sites with suspicion for tumor lesion based on white light and visible red fluorescence, and a control blind biopsy from the visually unchanged and non-fluorescent mucous tissue in each patient. The results of the study indicate the high effectiveness of the developed FD methodology with 5-ALA HE in detecting non-muscularinvasive bladder cancer during intravesical administration of the drug, due to selective accumulation of hexasens-induced PPIX in the tumor tissue compared with healthy mucosa. Compared with the results of standard cystoscopy, fluorescence diagnostics significantly increased diagnostic sensitivity by 24.4% (from 75.1% to 99.5%), diagnostic accuracy – by 15.8% (from 82.4% to 98.2%) and a negative predictive value – by 33.2% (from 65.8% to 99%) (p≤0.05). Additionally, a total of 37 (33.6%) patients was found to have 63 foci of fluorescence with a diameter of 2.5 to 3.0 mm. 59 of these were morphologically confirmed to contain cancer cells.
本文介绍了一项临床研究的结果，该研究使用恶性肿瘤FD光敏剂- 5-氨基乙酰丙酸己基酯(5-ALA HE)荧光诊断(FD)与标准膀胱镜检查比较非肌肉浸润性膀胱癌的诊断效果。这项研究涉及110名患者。研究开始时，膀胱内给药50 ml 0.2%的5-ALA HE溶液，暴露时间为1小时，然后将药物从器官中取出。在接下来的一个小时内，用两种膀胱镜检查粘膜，然后根据白光和可见红色荧光对所有怀疑有肿瘤病变的尿路上皮部位进行标准经尿道切除术，并对每个患者的视觉不变和非荧光粘膜组织进行对照盲活检。该研究结果表明，由于六体素诱导的PPIX在肿瘤组织中选择性积累，与健康粘膜相比，在膀胱内给药期间，开发的FD方法与5-ALA HE在检测非肌肉侵袭性膀胱癌方面具有很高的有效性。与标准膀胱镜检查结果相比，荧光诊断的诊断敏感性提高了24.4%(从75.1%提高到99.5%)，诊断准确性提高了15.8%(从82.4%提高到98.2%)，阴性预测值提高了33.2%(从65.8%提高到99%)(p≤0.05)。此外，共有37例(33.6%)患者发现63个荧光灶，直径为2.5至3.0 mm，其中59例经形态学证实含有癌细胞。
{"title":"Improving the efficiency of bladder cancer diagnostic cystoscopy with 5-ALA hexyl ester","authors":"A. Kaprin, A. Trushin, M. P. Golovachenko, V. Ivanova-Radkevich, V. Chissov, E. Filonenko","doi":"10.24931/2413-9432-2019-8-1-29-37","DOIUrl":"https://doi.org/10.24931/2413-9432-2019-8-1-29-37","url":null,"abstract":"This article presents the results of a clinical study that examined the diagnostic efficacy of fluorescent diagnostics (FD) of non-muscularinvasive bladder cancer using a photosensitizer of FD of malignant neoplasms – 5-aminolevulinic acid hexyl ester (5-ALA HE) compared with standard cystoscopy. The study involved 110 patients. The study began with intravesical administration of 50 ml of 0.2% solution of 5-ALA HE, the exposure time was 1 hour, after which the drug was removed from the organ. During the next hour, the mucous membranes were examined in two cystoscopy modes, followed by a standard transurethral resection of all urothelium sites with suspicion for tumor lesion based on white light and visible red fluorescence, and a control blind biopsy from the visually unchanged and non-fluorescent mucous tissue in each patient. The results of the study indicate the high effectiveness of the developed FD methodology with 5-ALA HE in detecting non-muscularinvasive bladder cancer during intravesical administration of the drug, due to selective accumulation of hexasens-induced PPIX in the tumor tissue compared with healthy mucosa. Compared with the results of standard cystoscopy, fluorescence diagnostics significantly increased diagnostic sensitivity by 24.4% (from 75.1% to 99.5%), diagnostic accuracy – by 15.8% (from 82.4% to 98.2%) and a negative predictive value – by 33.2% (from 65.8% to 99%) (p≤0.05). Additionally, a total of 37 (33.6%) patients was found to have 63 foci of fluorescence with a diameter of 2.5 to 3.0 mm. 59 of these were morphologically confirmed to contain cancer cells.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68973829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-31DOI: 10.24931/2413-9432-2019-8-1-18-23
E. Plotnikova, V. O. Stramova, N. Morozova, A. Plyutinskaya, P. Ostroverkhov, M. Grin, A. Mironov, R. Yakubovskaya, A. Kaprin
The aim of the paper was to obtain a stable micellar emulsion of potent photosensitizer (PS) –O-propyloxime-N-propoxybacteriopurpurinimide methyl ester absorbing light in long-wave region of the spectrum (λmax= 800±2 нм). Solubilizates of the dye based on different surfactants (Kolliphor ELP, Poloxamer 407, Emuxol 268) were obtained. Taking into account the physical and chemical parameters, the most potent micellar emulsion for injection was selected and characterized. The emulsion based on Kolliphor ELP remains stable for 4 months, with no changes in the fluorescence spectrum and absorption, as well as the particle diameter.
本文的目的是制备一种稳定的强效光敏剂(PS) - o -丙氧基肟- n -丙氧基细菌嘌呤酰亚胺甲酯胶束乳液，在光谱的长波区(λmax= 800±2 нм)吸收光。得到了不同表面活性剂(Kolliphor ELP, poloxam407, Emuxol 268)对染料的增溶剂。综合考虑理化参数，选择了最有效的注射用胶束乳液，并对其进行了表征。基于Kolliphor ELP的乳液在4个月内保持稳定，荧光光谱和吸收没有变化，颗粒直径也没有变化。
{"title":"Solubilization of hydrophobic bacteriochlorin-based photosensitizers in micelles of surfactants","authors":"E. Plotnikova, V. O. Stramova, N. Morozova, A. Plyutinskaya, P. Ostroverkhov, M. Grin, A. Mironov, R. Yakubovskaya, A. Kaprin","doi":"10.24931/2413-9432-2019-8-1-18-23","DOIUrl":"https://doi.org/10.24931/2413-9432-2019-8-1-18-23","url":null,"abstract":"The aim of the paper was to obtain a stable micellar emulsion of potent photosensitizer (PS) –O-propyloxime-N-propoxybacteriopurpurinimide methyl ester absorbing light in long-wave region of the spectrum (λmax= 800±2 нм). Solubilizates of the dye based on different surfactants (Kolliphor ELP, Poloxamer 407, Emuxol 268) were obtained. Taking into account the physical and chemical parameters, the most potent micellar emulsion for injection was selected and characterized. The emulsion based on Kolliphor ELP remains stable for 4 months, with no changes in the fluorescence spectrum and absorption, as well as the particle diameter.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68973776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-14DOI: 10.24931/2413-9432-2018-7-4-35-40
В. М. Легостаев, О. Ю. Бабенков, Г. В. Балицкий
We present a clinical case with a complete endoscopic and clinical effect after endoscopic treatment of a patient with laryngeal cancer involving the upper third of the esophagus. The patient was treated as follows: conformal radiation therapy TFD = 40 gr, targeted chemotherapy using Cetuximab (total dose of 1800 mg). 1.5 months after the end of the treatment, a residual laryngopharyngeal tumor with a spread into the upper third of the esophagus was found during videolaryngoscopy examination. The result of the following histological examination was G2 squamous cell carcinoma. From August 2015 to February 2017, the patient underwent 8 photodynamic therapy sessions in combination with argon plasma coagulation. A control videolaryngoscopy, carried out 1 month after the fnal session, showed complete tumor regression without cicatricial deformity and narrowing of the esophageal lumen.
{"title":"Комбинированное эндоскопическое лечение больного раком гортаноглотки с распространением на верхнюю треть пищевода","authors":"В. М. Легостаев, О. Ю. Бабенков, Г. В. Балицкий","doi":"10.24931/2413-9432-2018-7-4-35-40","DOIUrl":"https://doi.org/10.24931/2413-9432-2018-7-4-35-40","url":null,"abstract":"We present a clinical case with a complete endoscopic and clinical effect after endoscopic treatment of a patient with laryngeal cancer involving the upper third of the esophagus. The patient was treated as follows: conformal radiation therapy TFD = 40 gr, targeted chemotherapy using Cetuximab (total dose of 1800 mg). 1.5 months after the end of the treatment, a residual laryngopharyngeal tumor with a spread into the upper third of the esophagus was found during videolaryngoscopy examination. The result of the following histological examination was G2 squamous cell carcinoma. From August 2015 to February 2017, the patient underwent 8 photodynamic therapy sessions in combination with argon plasma coagulation. A control videolaryngoscopy, carried out 1 month after the fnal session, showed complete tumor regression without cicatricial deformity and narrowing of the esophageal lumen.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"7 1","pages":"35-40"},"PeriodicalIF":0.0,"publicationDate":"2019-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49543736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-14DOI: 10.24931/2413-9432-2018-7-4-23-34
I. Osmakov, T. Savelieva, V. Loschenov, S. Goryajnov, A. Potapov
The paper presents the results of a comparative study of methods of cluster analysis of optical intraoperative spectroscopy data during surgery of glial tumors with varying degree of malignancy. The analysis was carried out both for individual patients and for the entire dataset. The data were obtained using combined optical spectroscopy technique, which allowed simultaneous registration of diffuse reflectance spectra of broadband radiation in the 500–600 nm spectral range (for the analysis of tissue blood supply and the degree of hemoglobin oxygenation), fluorescence spectra of 5‑ALA induced protoporphyrin IX (Pp IX) (for analysis of the malignancy degree) and signal of diffusely reflected laser light used to excite Pp IX fluorescence (to take into account the scattering properties of tissues). To determine the threshold values of these parameters for the tumor, the infltration zone and the normal white matter, we searched for the natural clusters in the available intraoperative optical spectroscopy data and compared them with the results of the pathomorphology. It was shown that, among the considered clustering methods, EM‑algorithm and k‑means methods are optimal for the considered data set and can be used to build a decision support system (DSS) for spectroscopic intraoperative navigation in neurosurgery. Results of clustering relevant to thepathological studies were also obtained using the methods of spectral and agglomerative clustering. These methods can be used to postprocess combined spectroscopy data.
{"title":"Cluster analysis of the results of intraoperative optical spectroscopic diagnostics In brain glioma neurosurgery","authors":"I. Osmakov, T. Savelieva, V. Loschenov, S. Goryajnov, A. Potapov","doi":"10.24931/2413-9432-2018-7-4-23-34","DOIUrl":"https://doi.org/10.24931/2413-9432-2018-7-4-23-34","url":null,"abstract":"The paper presents the results of a comparative study of methods of cluster analysis of optical intraoperative spectroscopy data during surgery of glial tumors with varying degree of malignancy. The analysis was carried out both for individual patients and for the entire dataset. The data were obtained using combined optical spectroscopy technique, which allowed simultaneous registration of diffuse reflectance spectra of broadband radiation in the 500–600 nm spectral range (for the analysis of tissue blood supply and the degree of hemoglobin oxygenation), fluorescence spectra of 5‑ALA induced protoporphyrin IX (Pp IX) (for analysis of the malignancy degree) and signal of diffusely reflected laser light used to excite Pp IX fluorescence (to take into account the scattering properties of tissues). To determine the threshold values of these parameters for the tumor, the infltration zone and the normal white matter, we searched for the natural clusters in the available intraoperative optical spectroscopy data and compared them with the results of the pathomorphology. It was shown that, among the considered clustering methods, EM‑algorithm and k‑means methods are optimal for the considered data set and can be used to build a decision support system (DSS) for spectroscopic intraoperative navigation in neurosurgery. Results of clustering relevant to thepathological studies were also obtained using the methods of spectral and agglomerative clustering. These methods can be used to postprocess combined spectroscopy data.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48430580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-14DOI: 10.24931/2413-9432-2018-7-4-16-22
A. Budko, Z. G. Deichman, G. Meerovich, L. Borisova, I. G. Мeerovich, A. Lantsova, Kulbachevskaya N.Yu. Kulbachevskaya N.Yu. Kulbachevskaya N.Yu.
The present work is devoted to the study of pharmacokinetics of infrared photosensitizer (PS) based on hydroxyaluminium tetra‑3‑phenylthiophthalocyanine in a sterically stabilized liposomal form. The study was carried out on adult female mice. The PS was administered once intravenously at a dose of 6 mg / kg. Evaluation of the PS accumulation dynamics in the mice tissues and organs was performed at time intervals from 5 minutes to 7 days using spectral‑fluorescent method. The maximum accumulation of the PS photoactive form was recorded in lungs (32 µg / g in the interval of 5–30 minutes after introduction), liver (20.8 µg / g in the interval of 4–24 hours after introduction) and spleen (28 µg / g 4 hours after introduction). At the same time, by the end of the observation period (7 days after administration), trace amounts of the PS photoactive form were still detected in the liver and the spleen at a calculated concentration of 0.5‑1 µg / g. The PS accumulated the least in muscles and skin. The fluorescent signal from the PS accumulated in skin was detectable almost immediately, and its concentration remained at the same level (1.2‑1.5 µg / g) for up to 3 days of observation. In the muscles, the concentration of the PS reached 1.5 µg / g 15 minutes after administration, and then gradually decreased until 0.25 µg / g at 24 hours. Data on the pharmacokinetics of PS in blood, basic organs and tissues of animals were obtained, pharmacokinetic parameters were calculated. 7 days after the administration, the PS concentration in the skin and muscles was below the detection limit. The studies confrmed that PEGylation of the PS liposomal form slows down the process of its capture by reticulo‑endothelial system. It was shown that the PS circulates in blood and organs of mice for a long time and it completely distributes only when 4 hours pass after administration.
{"title":"Study of pharmacokinetics of liposomal photosensitiser based on hydroxyaluminium tetra-3-phenylthiophthalocyanine on mice","authors":"A. Budko, Z. G. Deichman, G. Meerovich, L. Borisova, I. G. Мeerovich, A. Lantsova, Kulbachevskaya N.Yu. Kulbachevskaya N.Yu. Kulbachevskaya N.Yu.","doi":"10.24931/2413-9432-2018-7-4-16-22","DOIUrl":"https://doi.org/10.24931/2413-9432-2018-7-4-16-22","url":null,"abstract":"The present work is devoted to the study of pharmacokinetics of infrared photosensitizer (PS) based on hydroxyaluminium tetra‑3‑phenylthiophthalocyanine in a sterically stabilized liposomal form. The study was carried out on adult female mice. The PS was administered once intravenously at a dose of 6 mg / kg. Evaluation of the PS accumulation dynamics in the mice tissues and organs was performed at time intervals from 5 minutes to 7 days using spectral‑fluorescent method. The maximum accumulation of the PS photoactive form was recorded in lungs (32 µg / g in the interval of 5–30 minutes after introduction), liver (20.8 µg / g in the interval of 4–24 hours after introduction) and spleen (28 µg / g 4 hours after introduction). At the same time, by the end of the observation period (7 days after administration), trace amounts of the PS photoactive form were still detected in the liver and the spleen at a calculated concentration of 0.5‑1 µg / g. The PS accumulated the least in muscles and skin. The fluorescent signal from the PS accumulated in skin was detectable almost immediately, and its concentration remained at the same level (1.2‑1.5 µg / g) for up to 3 days of observation. In the muscles, the concentration of the PS reached 1.5 µg / g 15 minutes after administration, and then gradually decreased until 0.25 µg / g at 24 hours. Data on the pharmacokinetics of PS in blood, basic organs and tissues of animals were obtained, pharmacokinetic parameters were calculated. 7 days after the administration, the PS concentration in the skin and muscles was below the detection limit. The studies confrmed that PEGylation of the PS liposomal form slows down the process of its capture by reticulo‑endothelial system. It was shown that the PS circulates in blood and organs of mice for a long time and it completely distributes only when 4 hours pass after administration.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68974106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-14DOI: 10.24931/2413-9432-2018-7-4-11-15
V. Kravtsov, E. S. Pirozhenko, K. V. Pavelec, M. A. Protchenkov, U. A. Drozd
Cytological studies on lymph nodes of abdominal lymphodissection zone after local intraoperative photodynamic therapy (IOPDT) of gastrointestinal cancers were carried out. As a result of the PDT, the metastatic cells are destroyed, their cytoplasmic membranes and the cytoplasm disappears, leaving behind interphase nuclei ("naked nuclei") (p<0,0001). Cytological confrmation of apoptosis (the presence of apoptotic bodies) in metastatic lymph nodes after IOPDT sessions on the lymph nodes of the abdominal lymphodissection zone is also presented.
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