Asyeih Sabernejad, Abdolhadi Bashar, Marhab Rihan, N. Kazem, M. Hassanshahian
Introduction: Diesel oil is the most used petroleum product in Iran and other countries. The majority of diesel oil is stored in underground reservoirs and Fuel stations. This product can heavily pollute the adjacent soil. Diesel oil pollution has some ecological effects on soil that disturb the composition and diversity of the microbial community. The present research aimed to investigate the effects of diesel oil pollution on two different types of soil. Materials and Methods: To examine the effects of diesel oil on microbial communities, two different types of soil (industrial and forest types) were collected from Kerman province, Iran. Six microcosms were designed based on three microcosms existing in each type of soil, including unpolluted microcosm, polluted microcosm, and polluted microcosm with nutrients (Nitrogen and Phosphor). Some factors were assayed in each microcosm during 120 days of the experiment. These factors included total heterotrophic bacteria, total diesel oil-degrading bacteria, dehydrogenase enzyme, and diesel oil biodegradation. Results: The quantity of diesel oil-degrading bacteria was significantly lower than heterotrophic bacteria in all soil microcosms. The quantity of diesel oil-degrading bacteria had a decrement pattern until day 60 of the experiment, but after that, these bacteria had an increment pattern. The best dehydrogenase activity between different microcosms was related to polluting microcosms with diesel oil except for farmland soil. The highest biodegradation of diesel oil in all studied soil types belonged to the industrial microcosm (95%). Statistical analysis of the results indicated a significant correlation between the most probable number quantity of heterotrophic bacteria and other assayed factors. Forest soil was significantly different from other soil types. Conclusion: Given the obtained results of the current research, that forest soil is more sensitive to diesel oil pollution, compared to industrial soil. It is, therefore, possible to propose appropriate strategies for the bioremediation of different studied soil types.
{"title":"Comparing the Effects of Diesel Oil Pollution on Forest and Industrial Soil Microbial Community","authors":"Asyeih Sabernejad, Abdolhadi Bashar, Marhab Rihan, N. Kazem, M. Hassanshahian","doi":"10.58803/rbes.v1i1.2","DOIUrl":"https://doi.org/10.58803/rbes.v1i1.2","url":null,"abstract":"Introduction: Diesel oil is the most used petroleum product in Iran and other countries. The majority of diesel oil is stored in underground reservoirs and Fuel stations. This product can heavily pollute the adjacent soil. Diesel oil pollution has some ecological effects on soil that disturb the composition and diversity of the microbial community. The present research aimed to investigate the effects of diesel oil pollution on two different types of soil. \u0000Materials and Methods: To examine the effects of diesel oil on microbial communities, two different types of soil (industrial and forest types) were collected from Kerman province, Iran. Six microcosms were designed based on three microcosms existing in each type of soil, including unpolluted microcosm, polluted microcosm, and polluted microcosm with nutrients (Nitrogen and Phosphor). Some factors were assayed in each microcosm during 120 days of the experiment. These factors included total heterotrophic bacteria, total diesel oil-degrading bacteria, dehydrogenase enzyme, and diesel oil biodegradation. \u0000Results: The quantity of diesel oil-degrading bacteria was significantly lower than heterotrophic bacteria in all soil microcosms. The quantity of diesel oil-degrading bacteria had a decrement pattern until day 60 of the experiment, but after that, these bacteria had an increment pattern. The best dehydrogenase activity between different microcosms was related to polluting microcosms with diesel oil except for farmland soil. The highest biodegradation of diesel oil in all studied soil types belonged to the industrial microcosm (95%). Statistical analysis of the results indicated a significant correlation between the most probable number quantity of heterotrophic bacteria and other assayed factors. Forest soil was significantly different from other soil types. \u0000Conclusion: Given the obtained results of the current research, that forest soil is more sensitive to diesel oil pollution, compared to industrial soil. It is, therefore, possible to propose appropriate strategies for the bioremediation of different studied soil types.","PeriodicalId":385847,"journal":{"name":"Research in Biotechnology and Environmental Science","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125410168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Salari, Z. Bayat, S. Dabaghi, Fatemeh Salahshoori Niaei
Introduction: Increasing the concentration of Boron in drinking water, wastewater, and irrigation have negative effects on the human environment. This pollution can be partially removed by the application of phytoremediation technologies using algae or aquatic plants. The aim of the current study was to determine the biosorption capacity of the algae Spirogyra sp. for Boron from industrial wastewater and examine the best elimination conditions using different parameters. Materials and Methods: In this study, 100 g of fresh algal biomass was collected from the industrial wastewater of a copper mine located in Kerman, Iran. At first, algae was selected among various algal species concerning abundance and resistance ability to high concentrations of Boron. Then, removal of Boron by the algal was examined in terms of algae biomass levels (2 and 4 gr), incubation time intervals (2, 12. 24. 48, and 72 hours), and different concentrations of Boron (5, 10, 15, 25, and 100 ppm) on the were examined. The experiment was factorial with a completely randomized design framework and three replications. Results: The results presented that the elimination of Boron from industrial wastewater was performed by biomass of algae Spirogyra sp. The maximum Boron absorption was achieved at concentrations of 5 ppm and an incubation time of 12 hours. The absorption of Boron was higher in 4 gr than in 2 gr of algae biomass treatment. Conclusion: It can be concluded that algae Spirogyra sp. has a strong potential for boron removal in industrial wastewater containing boron ions.
{"title":"Biosorption of Boron from Industrial Wastewater by Green Algae Spirogyra sp.","authors":"Z. Salari, Z. Bayat, S. Dabaghi, Fatemeh Salahshoori Niaei","doi":"10.58803/rbes.v1i1.3","DOIUrl":"https://doi.org/10.58803/rbes.v1i1.3","url":null,"abstract":"Introduction: Increasing the concentration of Boron in drinking water, wastewater, and irrigation have negative effects on the human environment. This pollution can be partially removed by the application of phytoremediation technologies using algae or aquatic plants. The aim of the current study was to determine the biosorption capacity of the algae Spirogyra sp. for Boron from industrial wastewater and examine the best elimination conditions using different parameters. \u0000Materials and Methods: In this study, 100 g of fresh algal biomass was collected from the industrial wastewater of a copper mine located in Kerman, Iran. \u0000At first, algae was selected among various algal species concerning abundance and resistance ability to high concentrations of Boron. Then, removal of Boron by the algal was examined in terms of algae biomass levels (2 and 4 gr), incubation time intervals (2, 12. 24. 48, and 72 hours), and different concentrations of Boron (5, 10, 15, 25, and 100 ppm) on the were examined. The experiment was factorial with a completely randomized design framework and three replications. \u0000Results: The results presented that the elimination of Boron from industrial wastewater was performed by biomass of algae Spirogyra sp. The maximum Boron absorption was achieved at concentrations of 5 ppm and an incubation time of 12 hours. The absorption of Boron was higher in 4 gr than in 2 gr of algae biomass treatment. \u0000Conclusion: It can be concluded that algae Spirogyra sp. has a strong potential for boron removal in industrial wastewater containing boron ions.","PeriodicalId":385847,"journal":{"name":"Research in Biotechnology and Environmental Science","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128532946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Urinary tract infection (UTI) is a bacterial infection that affects the urinary tract. The bacterial infection of the upper urinary tract is called nephritis. The purpose of this study was to evaluate the antibacterial effect of Valeriana officinalis and Ciprofloxacin on kidney histopathology in rats with pyelonephritis by Pseudomonas. Materials and Methods: In this study, 48 male Wistar rats were divided into six groups and each group had two replicates. The first group was considered a control and received 0.1 mg/ kg/day of saline daily for a month. The second group received a single injection of 0.5 McFarland of microbial suspension per kilogram of animal into the pelvis of the right kidney of the rats, resulting in pyelonephritis. The third group of rats received 0.9 mg/kg/day methanolic extract of Valeriana officinalis intraperitoneally for a month. The fourth group of rats received Ciprofloxacin intraperitoneally for a month at a dose of 0.6 mg/kg/day. Group five contained rats with pyelonephritis that received the antibiotic (Ciprofloxacin) at a dosage of 0.6 mg/kg/day. Group six with pyelonephritis received 0.9 mg/kg/day of Valeriana officinalis extract. The inflammation in the cortex, Pelvic, medulla, and tissue sections was studied at the end of the study. Results: The rats that received Valeriana officinalis extract improved pelvic and medullary tissue, the site of Pseudomonas bacteria, and prevented the destruction of renal cortex tissue. The rats that received Ciprofloxacin had fewer medullary and tissue inflammations. Conclusion: According to the results of this research, the extract not only improved the tissue of the pelvis and medulla, which is where Pseudomonas bacteria live, but it also inhibits the degradation of renal cortex tissue. It was proven to diminish medullary inflammation to some extent, but in rats, it exacerbated the loss of renal cortex tissue.
{"title":"Effects of Valeriana officinalis and Ciprofloxacin on Kidney Histopathology in Rats Pyelonephritis by Pseudomonas aeruginosa","authors":"Fatemeh Salahshoori Niaei, Akbar Farah Taj Navab","doi":"10.58803/rbes.v1i1.5","DOIUrl":"https://doi.org/10.58803/rbes.v1i1.5","url":null,"abstract":"Introduction: Urinary tract infection (UTI) is a bacterial infection that affects the urinary tract. The bacterial infection of the upper urinary tract is called nephritis. The purpose of this study was to evaluate the antibacterial effect of Valeriana officinalis and Ciprofloxacin on kidney histopathology in rats with pyelonephritis by Pseudomonas. \u0000Materials and Methods: In this study, 48 male Wistar rats were divided into six groups and each group had two replicates. The first group was considered a control and received 0.1 mg/ kg/day of saline daily for a month. The second group received a single injection of 0.5 McFarland of microbial suspension per kilogram of animal into the pelvis of the right kidney of the rats, resulting in pyelonephritis. The third group of rats received 0.9 mg/kg/day methanolic extract of Valeriana officinalis intraperitoneally for a month. The fourth group of rats received Ciprofloxacin intraperitoneally for a month at a dose of 0.6 mg/kg/day. Group five contained rats with pyelonephritis that received the antibiotic (Ciprofloxacin) at a dosage of 0.6 mg/kg/day. Group six with pyelonephritis received 0.9 mg/kg/day of Valeriana officinalis extract. The inflammation in the cortex, Pelvic, medulla, and tissue sections was studied at the end of the study. \u0000Results: The rats that received Valeriana officinalis extract improved pelvic and medullary tissue, the site of Pseudomonas bacteria, and prevented the destruction of renal cortex tissue. The rats that received Ciprofloxacin had fewer medullary and tissue inflammations. \u0000Conclusion: According to the results of this research, the extract not only improved the tissue of the pelvis and medulla, which is where Pseudomonas bacteria live, but it also inhibits the degradation of renal cortex tissue. It was proven to diminish medullary inflammation to some extent, but in rats, it exacerbated the loss of renal cortex tissue.","PeriodicalId":385847,"journal":{"name":"Research in Biotechnology and Environmental Science","volume":"233 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114430439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-01DOI: 10.58803/rbes.2022.1.1.06
R. Sahebi, Nazanin Akbari, Z. Bayat, Mohammad Rashidmayvan, Amin Mansoori, M. Beihaghi
Autophagy is a well-known vital process in cells and plays a significant role in biological evolution, the immune system, and cell death. It can be effective in fatal disorders, such as nervous system degeneration, autoimmune diseases, and cancer. Autophagy has a dual role; on the one hand, it increases cell survival, and on the other hand, it causes cell death in advanced stages although no agreement has yet been accomplished on the role of autophagy in cellular processes. There is evidence that autophagic signaling regulation is inversely related to oncogenic signaling. Numerous commonly activated oncogenes (class I PtdIns3K, Akt, TOR, Bcl-2) inhibit autophagy, while commonly mutated or epigenetically silenced tumor suppressor genes (p53, PTEN, TSC1/TSC2) promote autophagy. Autophagy promotes cancer progression by supplying sufficient nutrients that enable cancer cell growth. FIP200, a related- autophagy protein, interacts with ATG 13 and induces autophagy. Increased autophagy causes the interaction of Becklin 118 with HER2, resulting in an increase in tumorigenesis. In order to make complete use of the autophagic properties in cancer treatment, further studies on its role in disease in the different biologics fields are essential. Cancer stem cells (CSCs) can regenerate, cause cancer, and enhance resistance to treatment, metastasis, and recurrence. Autophagy moderates stressful conditions and promotes resistance to anticancer therapy. In addition, autophagy regulates the ability of radiation in CSCs and leads to failure in anticancer therapies. Hence, autophagy is a potential therapeutic target for metastasis resistance and anticancer therapy recurrence. Regulation of autophagy using autophagy modulators alone does not improve the therapeutic effects of anticancer reagents. In contrast, it has supplied nutrients for cancer cells. Consequently, clinical trials aiming for autophagy through a combination of autophagy modulations and anticancer reagents are crucial to consider autophagy as a potentially effective therapeutic strategy in anticancer therapy.
{"title":"A Summary of Autophagy Mechanisms in Cancer Cells","authors":"R. Sahebi, Nazanin Akbari, Z. Bayat, Mohammad Rashidmayvan, Amin Mansoori, M. Beihaghi","doi":"10.58803/rbes.2022.1.1.06","DOIUrl":"https://doi.org/10.58803/rbes.2022.1.1.06","url":null,"abstract":"Autophagy is a well-known vital process in cells and plays a significant role in biological evolution, the immune system, and cell death. It can be effective in fatal disorders, such as nervous system degeneration, autoimmune diseases, and cancer. Autophagy has a dual role; on the one hand, it increases cell survival, and on the other hand, it causes cell death in advanced stages although no agreement has yet been accomplished on the role of autophagy in cellular processes. There is evidence that autophagic signaling regulation is inversely related to oncogenic signaling. Numerous commonly activated oncogenes (class I PtdIns3K, Akt, TOR, Bcl-2) inhibit autophagy, while commonly mutated or epigenetically silenced tumor suppressor genes (p53, PTEN, TSC1/TSC2) promote autophagy. Autophagy promotes cancer progression by supplying sufficient nutrients that enable cancer cell growth. FIP200, a related- autophagy protein, interacts with ATG 13 and induces autophagy. Increased autophagy causes the interaction of Becklin 118 with HER2, resulting in an increase in tumorigenesis. In order to make complete use of the autophagic properties in cancer treatment, further studies on its role in disease in the different biologics fields are essential. Cancer stem cells (CSCs) can regenerate, cause cancer, and enhance resistance to treatment, metastasis, and recurrence. Autophagy moderates stressful conditions and promotes resistance to anticancer therapy. In addition, autophagy regulates the ability of radiation in CSCs and leads to failure in anticancer therapies. Hence, autophagy is a potential therapeutic target for metastasis resistance and anticancer therapy recurrence. Regulation of autophagy using autophagy modulators alone does not improve the therapeutic effects of anticancer reagents. In contrast, it has supplied nutrients for cancer cells. Consequently, clinical trials aiming for autophagy through a combination of autophagy modulations and anticancer reagents are crucial to consider autophagy as a potentially effective therapeutic strategy in anticancer therapy.","PeriodicalId":385847,"journal":{"name":"Research in Biotechnology and Environmental Science","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131741618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：