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Further Evaluation of the performance of Shugor, Dubasi and Watish subtypes of Sudanese Desert sheep: Under Sedentary and Improved Production Systems 苏丹沙漠羊Shugor、Dubasi和Watish亚型生产性能的进一步评价:在定居和改进的生产系统下
Pub Date : 2022-09-01 DOI: 10.31559/vmph2022.3.3.3
A. Sulieman, A. Bashar, A. Elamin, H.A. El Tahir, S. Sulieman
The present study was conducted in the National Sheep Research Station in Elhuda, Sudan where the sheep flocks are kept for scientific research purposes. The aim of the study is to evaluate two systems of sheep productivity namely: the prevailing annual single lamb cropping (sheer-sedentary) and induced three lamb cropping per two years to which feed supplementation is provided (improved – sedentary). Three subtypes: Shugor, Dubasi and Watish in Sudan Desert sheep were used for a three –lamp crops, per two years, in addition to feed supplementation, to represent an improved-sedentary system, (B) against a sheer sedentary (A), that represents an annual single-lamp-crop system. The improved sedentary was associated with reductions in the overall average values of the traits: ewes losses (1.95 vs 3.09%), ewe barrenness (17.30 vs 28.67%) and lambing interval (257.97 vs 353.50 days ), whereas it enhanced lambing rate (82.73 vs72.67 %), litter size at weaning measured: as lambs weaned per ewes exposed for breeding (1.01 vs 0.67) and as lambs weaned per ewes that actually lambed (1.12 vs 0.93) improved adjusted lamb weaning weight per ewe at 60 days of lamb age (13.62 vs 10.58 kg) , increased lamb survival rate (93.91 vs 75.63%) and eventually promoted productivity index (18.36 vs 5.25kg) of lambs weaned per ewe per year. These overall average values were calculated out of the mean values of the measured traits shown in tables (1 and 2) of the text. Furthermore, the comparison between the three subtype sheep groups for their performance as affected by the improved sedentary against the sheer - sedentary revealed similar trends of response as above, that culminated in the Shugor subtype having the greatest productivity index mean value (20.82 kg) as subsequently compared with that of either the subtype Dubasi (17.56 kg) or the Watish subtype (16.70 kg). It is concluded that these results do encourage the adoption and projection on the improved-sedentary system, specially, under the surroundings of irrigated agriculture land use to provide extra income to the welfare of the farmer.
本研究是在苏丹埃尔胡达的国家绵羊研究站进行的,那里饲养的羊群用于科学研究目的。该研究的目的是评估两种绵羊生产系统,即:普遍的每年一次羔羊种植(纯放牧)和每两年诱导三次羔羊种植,并提供饲料补充(改进-放牧)。三种亚型:苏丹沙漠羊Shugor、Dubasi和Watish,除饲料补充外,每两年进行三灯作物种植,代表改进的久坐系统(B)与纯粹久坐系统(a),代表每年一次单灯作物系统。久坐的改善与以下性状的总体平均值降低有关:母羊损失(1.95 vs 3.09%)、母羊不育(17.30 vs 28.67%)和产羔间隔(257.97 vs 353.50天),而它提高了产羔率(82.73 vs 72.67%),断奶时的产仔量测量:每只母羊的断奶羔羊数(1.01 vs 0.67)和每只母羊的断奶羔羊数(1.12 vs 0.93)提高了60日龄每只母羊的调整断奶重(13.62 vs 10.58 kg),提高了羔羊成活率(93.91 vs 75.63%),最终提高了每只母羊每年断奶羔羊的生产指数(18.36 vs 5.25kg)。这些总体平均值是根据文本表(1和2)中所示的测量特征的平均值计算出来的。此外,对三种亚型绵羊群体在改善久坐和完全久坐影响下的表现进行比较,发现了与上述相似的反应趋势,最终Shugor亚型具有最高的生产力指数平均值(20.82 kg),随后与Dubasi亚型(17.56 kg)或Watish亚型(16.70 kg)相比。结论是,这些结果确实鼓励采用和预测改进的定居系统,特别是在灌溉农业土地利用的环境下,为农民的福利提供额外的收入。
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引用次数: 0
Live Weight and Live Weight Changes and Some Relationships of Certain Reproductive Traits in the Breeding Female Shugor and Dubasi Sub-Types of Sudan Desert Sheep 苏丹沙漠羊舒戈尔亚型和杜巴斯亚型母羊的活重、活重变化及某些生殖性状的关系
Pub Date : 2022-09-01 DOI: 10.31559/vmph2022.3.3.1
A. Sulieman, A. Bashar, H.A. El Tahir, S. Sulieman
This study was carried out at the National Sheep Research Station in Elhuda, Sudan to investigate the situation of female sheep conditions while in gestation and to consult some aspects associated to pregnancy parameters and others on live weight were collected, processed and statistically tested in addition to variability consideration. Thus, live weight changes its variability and some relationships for certain reproductive traits in the Shugor and Dubasi subtypes of Sudanese Desert sheep ewes were studied from mating events to the immediate postpartum date. The overall live weight changes during this period resulted in an increase of 22.8 and 27.6% in the live weight of either of these two ewe groups ,respectively, but with an overall greater in live weight variability within the Shugor as contrasted with that within their contemporary Dubasi ewe group (11.33vs 8.27%, CV), having periodic mean value which ranges from 10.70 -13.5% CV and 7.2 – 8.5% CV, respectively, for the Shugor and Dubasi ewe groups. The combined contribution of the foetal lamb weights, foetal placenta fluids weights composed 20.0% for the Shugor and 17.9% for the Dubasi of the breeding (mating) live weight of each while the weight of the lamb at birth and that of the associated placenta had mean values of 11.1 ±0.06% and 1.23 ± 0.06, respectively, of the Shugor pre-partum ewe live weight but the corresponding mean proportional values in the Dubasi group were 9.48 ± 0.49% and 1.20 ±0.08 % .The regression of gestation length, post-partum ewe live weight and lamb birth weight had mean values of + 0.37 , + 0.71 and 0.002 as related to the breeding (mating) ewe live weight of the Shugor whereas the corresponding regression values in the Dubasi ewes were +0.03 ,+ 0.67 and + 0.03. None of these contrasting parameter values was significantly different in the two subtype groups. Similarly, the regression values of the placenta weight on the lamb birth weight were not significantly different, respecttively, - 0.02 and + 0.032 in the Shugor and Dubasi ewe groups. It is concluded that live weights variations in breeding females should be made manageable to their least possible to allow for more solid data, on the female and its offspring, as this is conductive to performance development.
本研究是在苏丹Elhuda的国家绵羊研究站进行的,目的是调查母羊妊娠期的状况,并咨询与妊娠参数有关的一些方面,除了考虑变异性外,还收集、处理和统计检验了其他有关活重的数据。因此,研究了苏丹沙漠羊Shugor和Dubasi亚型母羊从交配事件到产后的某些生殖性状之间的活重变化及其变异性。在此期间,总体活重变化导致这两个母羊组的活重分别增加22.8%和27.6%,但与同时代杜巴斯母羊组相比,舒戈尔母羊组的总体活重变化率更大(11.33vs 8.27%, CV),舒戈尔和杜巴斯母羊组的周期性平均值分别为10.70 -13.5% CV和7.2 - 8.5% CV。绵羊胎重、胎液重量对绵羊繁殖(配种)活重的贡献率分别为20.0%和17.9%,羔羊出生时体重和相关胎盘重量的平均值分别为11.1±0.06%和1.23±0.06%。舒戈尔母羊妊娠期长、产后母羊活重和羔羊初生重与种(交)母羊活重相关的回归均值分别为+ 0.37、+ 0.71和0.002,而与杜巴斯母羊相关的回归均值分别为+0.03、+ 0.67和+0.03。这些对比参数值在两个亚型组中均无显著差异。同样,舒格尔母羊和杜巴斯母羊胎盘重量对羔羊出生体重的回归值也无显著差异,分别为- 0.02和+ 0.032。结论是,繁殖雌性的活重变化应尽可能控制在最低限度,以便获得关于雌性及其后代的更可靠的数据,因为这有助于性能发展。
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引用次数: 0
Assessment of the Effect of Sodium Hypochlorite, An Artificial Exsheathment Medium, on the Viability of Nematode Larvae 次氯酸钠人工培养培养基对线虫幼虫活力的影响
Pub Date : 2022-05-01 DOI: 10.31559/vmph2022.3.2.2
E. Elowni, G. H. Abdelnabi, M. Ahmad
Sodium hypochlorite (NaOCl) is used extensively as an artificial exsheathment medium of nematode larvae in various studies, such as comparing the efficacy of drugs, and assessment of resistance to anthelmintics or evaluation of plant extracts as anthelmintics due to its unique capacity for tissue dissolution. Studies with NaOCl by other authors indicate that the compound, although highly effective as an exsheathing agent, significantly lowers the infectivity of the exsheathed larvae produced, suggesting reduced viability. We used Strongyloides papillosus larvae, a nematode that naturally lacks a protective sheath and a potentially highly motile organism, as a model to exclude or confirm possible negative effects on the viability of the parasite. Motility was taken as a viability assay. Larvae were designated as actively motile, sluggish, or immotile. Results were presented as additional supplementary movie files. The viability of larvae is dependent on both the concentration and the time of exposure to the compound. There are certain concentration (C) and time (T) limits beyond which viability is impaired (0.3% ˃ C ˃ 0.2%; 10 min ˃ T ˃ 5 min). It is concluded that results from studies where NaOCl is used as an exsheathment medium should be interpreted with caution as the compound is capable of reducing the viability of larvae. It may even induce structural damage.
由于其独特的组织溶解能力,次氯酸钠(NaOCl)被广泛用作各种研究中线虫幼虫的人工出鞘培养基,如药物疗效比较、对驱虫药的抗性评估或植物提取物作为驱虫药的评估。其他作者对NaOCl的研究表明,该化合物虽然作为一种高效的脱毛剂,但显著降低了被脱毛的幼虫的传染性,表明生存能力降低。我们使用乳突圆线虫幼虫作为模型,以排除或确认可能对寄生虫生存能力产生的负面影响。乳突圆线虫是一种天然缺乏保护鞘的线虫,是一种潜在的高度运动性生物。以运动性为活力测定。幼虫被指定为活动活跃、迟缓或不活动。结果以附加的补充影片文件的形式呈现。幼虫的生存能力取决于暴露于化合物的浓度和时间。有一定的浓度(C)和时间(T)限制,超过了浓度(C)和时间(T),活力就会受损(0.3%);(10分钟)。因此,使用NaOCl作为脱毛培养基的研究结果应谨慎解释,因为该化合物能够降低幼虫的生存能力。它甚至可能导致结构损坏。
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引用次数: 0
Nutrient Intake and Digestibility of Red Sokoto Goats Fed Different Forms of Neem Leaves, Supplemented with a Concentrate Diet 不同形式印楝叶加精料饲粮对红索科托山羊营养物质摄入和消化率的影响
Pub Date : 2022-05-01 DOI: 10.31559/vmph2022.3.2.3
L. M. Ibrahim, J. Ocheja, B. Yahaya, M. Sadiq, B. Agyo, J. Uguru, I. Madu, M. Yahaya
The study evaluated the nutrient intake and digestibility of red sokoto goats fed different forms of neem (Azadirachta indica) leaves and a concentrate diet. The experiment was carried out at the small ruminant’s unit of the Teaching and Research Farm, Federal University of Kashere. Gombe state, Nigeria. Sixteen red sokoto goats (bucks) aged between 7 to 9 months, with weight range of 6.8 to 7.2 kg were allotted into four (4) treatments of four goats each. The goats were fed fresh neem leaves, neem leaves hay, neem leaves meal and neem leaves silage at 300g/goat/day for treatments 1, 2, 3 and 4 respectively; and concentrate diet at 125g/goat/day for a duration of sixty-three (63) days. Water was served ad-libitum. The goats were housed individually in pens the nutrient intake was calculated from the proximate composition of the diets and the neem leaves as well as the feed intake records. In the last 2 weeks of the experiment, the goats were fitted with harnesses forfeacal collection, a seven-day adjustment period was allowed for the goats to get used to the harnesses, feacal collection lasted for 7 days, the collected feaces were prepared appropriately and then analyzed for their proximate composition, using the method of AOAC, (2000), and thereafter used to calculate the dry matter and nutrient digestibility. The experimental design was a completely randomized design; data were analyzed using a one-way analysis of variance. The significant means were separated by Least Significant Difference using SAS 9.4, 2018 version statistical package. The concentrate and the neem leaves were analyzed for their proximate composition using the method of AOAC 2000. Values for nutrient intake were all not significantly (P>0.05) different except dry matter intake (46.18 – 51.45g/day/W 0.75).The digestibility values were not significantly (P<0.05) different except that of nitrogen free extracts (61.93 – 66. 79). Conclusively, fresh Neem leaves (T1) was the most preferred by the goats in terms of intake, it also had the best array of nutrients. The fresh neem leaves (T1) evaluated was recommended for feeding goats especially during the long dry season. Further research should be carried out using other species of ruminants such as sheep and cattle.
本研究评价了红索科托山羊饲喂不同形式印楝叶和精料日粮的营养摄入量和消化率。实验是在Kashere联邦大学教学和研究农场的小反刍动物单元进行的。尼日利亚贡贝州。16只7 - 9个月大、体重6.8 - 7.2公斤的红索科托山羊被分成4组,每组4只。处理1、2、3、4分别饲喂新鲜印楝叶、印楝叶干草、印楝叶粕和印楝叶青贮300g/只/d;精料饲粮剂量为125g/只/d,试验期63 d。水是随意供应的。将山羊单独圈养在围栏中,根据日粮的近似组成和印楝叶以及采食量记录计算营养摄入量。在试验的最后2周,对试验山羊进行套套收集,并给予7 d的调整期,使山羊适应套套,收集7 d的粪便,对收集到的粪便进行适当的处理,然后使用AOAC(2000)法分析其近似成分,然后计算干物质和营养物质消化率。实验设计为完全随机设计;数据分析采用单因素方差分析。采用sas9.4, 2018版统计软件包,以Least significant Difference分隔显著均值。用AOAC 2000法对浓缩物和印楝叶进行了近似成分分析。除干物质采食量(46.18 ~ 51.45g/d /W 0.75)外,各组营养物质采食量差异均不显著(P < 0.05)。除无氮提取物的消化率为61.93 ~ 66外,各组消化率差异不显著(P<0.05)。79)。综上所述,新鲜印楝叶(T1)是山羊最喜欢的摄取量,其营养成分也最好。所评价的鲜楝叶(T1)被推荐用于喂养山羊,特别是在漫长的旱季。进一步的研究应使用其他种类的反刍动物,如羊和牛。
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引用次数: 0
Seroprevalence and Risk Factors of Human Brucellosis and Toxoplasmosis in Ghazni Province of Afghanistan 阿富汗加兹尼省人类布鲁氏菌病和弓形虫病的血清患病率和危险因素
Pub Date : 2022-05-01 DOI: 10.31559/vmph2022.3.2.1
Sayed Sharif Abdali, A. Samadi
Brucellosis and toxoplasmosis are considered neglected zoonotic diseases with a worldwide distribution, which are endemic in Afghanistan. A cross-sectional study was carried out from May 24, 2021, to Feb 20, 2022 to determine the seroprevalence and risk factors associated with human brucellosis and toxoplasmosis in suspected patients admitted to the public and private laboratories of Ghazni province of Afghanistan. To detect Brucella IgM. a semi-quantitative agglutination test was used, while for the detection of Toxoplasma IgM and IgG, a chromatographic immunoassay was applied. Seroprevalence of brucellosis and toxoplasmosis were 32.1% (76/237) and 19.6% (18/92) in tested patients, respectively. Whereas, only in five cases dual infections were found. The majority of brucellosis positive cases were among the age group >45 years old (57.1%; p<0.05), in district residents (43.5%; p>0.05), and among those with abortion (30.6%; p<0.05) and history of positive brucellosis cases (50.0%; p<0.05) in their family members. High seropositive cases of toxoplasmosis were observed in males (50.0%; p>0.05), 21-45 years old people (20.8%; p>0.05), those who owned dogs and cats (p>0.05), among those with the history of abortion cases in their animals (41.7%; p<0.05), and positive toxoplasmosis cases in their family members (66.7.0%; p≤0.05). The odds of being seropositive for brucellosis was higher in those that had history of abortion (OR: 4.3; 95%CI: 1.3-4.2) and brucellosis cases (OR: 7.1; 95%CI: 1.7-28.9) among their family members. Furthermore, the odds of being seropositive for toxoplasmosis was 13.2 times more (95%CI: 2.1, 82.2; p<0.05) in those which had history of abortion cases in their animals. The high seroprevalence could be attributed to the study population being drawn from suspected cases rather than the general population. To assess the cross-connection of infections in human and animal populations, it would be more beneficial to study a large number of human and animal samples concurrently.
布鲁氏菌病和弓形虫病被认为是被忽视的人畜共患疾病,分布在世界各地,在阿富汗流行。从2021年5月24日至2022年2月20日开展了一项横断面研究,以确定阿富汗加兹尼省公立和私立实验室收治的疑似患者的人间布鲁氏菌病和弓形虫病的血清患病率和相关危险因素。检测布鲁氏菌IgM。采用半定量凝集试验,采用层析免疫法检测弓形虫IgM和IgG。布鲁氏菌病和弓形虫病的血清阳性率分别为32.1%(76/237)和19.6%(18/92)。而双重感染仅5例。布鲁氏菌病阳性病例以>45岁年龄组居多(57.1%;P0.05),流产组(30.6%;P0.05), 21 ~ 45岁(20.8%;P >0.05),养狗、养猫者(P >0.05),有动物流产史者占41.7%;P <0.05),家庭成员弓形虫病阳性病例占66.7.0%;p≤0.05)。有流产史的患者布鲁氏菌病血清阳性的几率更高(OR: 4.3;95%CI: 1.3-4.2)和布鲁氏菌病病例(OR: 7.1;95%CI: 1.7 ~ 28.9)。此外,弓形虫病血清阳性的几率是13.2倍(95%CI: 2.1, 82.2;P <0.05)。高血清阳性率可归因于研究人群来自疑似病例,而不是一般人群。为了评估人类和动物群体感染的交叉联系,同时研究大量的人类和动物样本将更有益。
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引用次数: 0
Occurrence of Aflatoxins in Livers and Milk of Camels in Saudi Arabia 沙特阿拉伯骆驼肝脏和牛奶中黄曲霉毒素的发生
Pub Date : 2022-01-01 DOI: 10.31559/vmph2022.3.1.1
F. Al-hizab, S. Barakat, Y. Hussein
The objective of the present study was to detect and quantify aflatoxins in liver and milk of camels. HPLC method was used to detect different aflatoxins. Liver samples were collected from slaughterhouses and milk samples from breeder's sites in Eastern Province, Saudi Arabia. Samples of camel livers and milk (250 samples of each) were collected and analyzed. About 26.4 and 16% of livers showed a contamination level in the range of 0.4-1 ng/g aflatoxin B1 and B2, respectively, but there were no detectable levels of G1, G2, M1 and M2. About 29.7% of milk samples had a range of aflatoxin M1 of 15-50 ng/l and 6% had a range of 50-100 ng/l. However, M2 was not detected in any milk sample. Based on regulatory limits, the present values of aflatoxin in livers and milk were within the internationally accepted limits (permissible limits of aflatoxins are 0.5 ppb for liver and liquid milk, according to the Saudi Organization for Standardization, Meteorology and Quality, (SASO).
本研究的目的是检测和量化骆驼肝脏和牛奶中的黄曲霉毒素。采用高效液相色谱法检测黄曲霉毒素。从沙特阿拉伯东部省的屠宰场收集肝脏样本,并从饲养场收集牛奶样本。收集和分析了骆驼肝脏和牛奶的样本(各250个样本)。黄曲霉毒素B1和B2在0.4-1 ng/g范围内的污染比例分别为26.4%和16%,G1、G2、M1和M2未检出。约29.7%的牛奶样品中黄曲霉毒素M1的范围为15-50 ng/l, 6%的牛奶样品中黄曲霉毒素M1的范围为50-100 ng/l。然而,牛奶样品中未检测到M2。根据监管限制,肝脏和牛奶中黄曲霉毒素的当前值在国际公认的限制范围内(根据沙特标准化、气象和质量组织(SASO)的数据,肝脏和液态牛奶中黄曲霉毒素的允许限量为0.5 ppb)。
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引用次数: 0
Urinary Schistosomiasis and its Associated Risk Factors Among Inhabitants of Internally Displaced Peoples’ (IDP) Camps in Maiduguri, North-eastern Nigeria: A Cross-sectional Study 尼日利亚东北部迈杜古里境内流离失所者(IDP)营地居民尿路血吸虫病及其相关危险因素:一项横断面研究
Pub Date : 2022-01-01 DOI: 10.31559/vmph2022.3.1.2
H. I. Musa, A. Muhammad, A. O. Tijjani, A. Saidu, M. M. Gashua, M. Goje, Deborah Tosin Ojo, Muhammad Auwal Idris
Urinary schistosomiasis is a tropical parasitic disease caused by Schistosoma haematobium and is usually associated with lesions in the genito-urinary tracts of affected people. A cross-sectional study was designed to examine factors influencing the occurrence of the disease among inhabitants of the IDP camps located in Maiduguri between October 2016 and June 2019. Information on demography, major sources of water supply and water-contact behaviours were obtained using pre-validated structured questionnaire. Urine samples were collected and examined for Schistosoma haematobium eggs using standard methods. A sample yielding at least one egg was considered positive while a sample that did not yield any was considered to be negative. The characteristics and disease risk factors were compared between respondents that tested positive and those that were negative for the infection using univariable statistical analysis and factors found to be significant were further analyzed using multivariable logistic regression. The results showed the overall prevalence of infection among the IDPs to be 21.9% (95% Confidence Interval, 95% CI= 19.54-24.42%). The multivariable logistic regression model showed that the statistically significant (p<0.05) predictors of infection including sex, occupation (c2 = 9.873, df=4, p=0.04), participation in water sporting activity and having washed/bathed in open water body before displacement, presence of signs of the disease in a tent/room member, knowledge of the disease and major source of water supply (c2 = 72.467, df=2, p<0.001) before the displacement. The relatively high prevalence of the disease in the study area may have negative effects on the reproduction and productivity of the IDPs. Based on the identified risk factors, behavioural changes, improved sanitation and safe water supply were recommended as control measures against urinary schistosomiasis in the study area.
尿路血吸虫病是一种由血血吸虫引起的热带寄生虫病,通常与患者的生殖-尿路病变有关。一项横断面研究旨在研究2016年10月至2019年6月期间迈杜古里境内流离失所者营地居民中疾病发生的影响因素。使用预先验证的结构化问卷获得了有关人口统计、主要供水来源和水接触行为的信息。收集尿液样本,并使用标准方法检查血血吸虫卵。产生至少一个卵子的样本被认为是阳性的,而没有产生任何卵子的样本被认为是阴性的。采用单变量统计分析比较感染阳性和阴性应答者的特征和疾病危险因素,并采用多变量logistic回归分析发现显著的因素。结果显示,境内流离失所者的总体感染率为21.9%(95%可信区间,95% CI= 19.54 ~ 24.42%)。多变量logistic回归模型显示,性别、职业(c2 = 9.873, df=4, p=0.04)、迁移前参加水上运动和在开放水域洗澡、在帐篷/房间成员中存在疾病迹象、对疾病的了解和主要供水来源(c2 = 72.467, df=2, p<0.001)是迁移前感染的有统计学意义(p<0.05)的预测因子。该疾病在研究地区相对较高的流行率可能对国内流离失所者的繁殖和生产力产生负面影响。根据已确定的危险因素,建议改变行为、改善卫生设施和安全供水作为研究地区尿路血吸虫病的控制措施。
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引用次数: 0
Occurrence of adulterants and preservatives in the Milk Sold in Rural areas of Omdurman, Sudan 苏丹恩图曼农村地区销售的牛奶中存在掺假和防腐剂
Pub Date : 2021-09-01 DOI: 10.31559/vmph2021.2.3.1
Asged, A. A. Mohammed, I. El Zubeir
In the present investigation, the chemical composition of milk was determined and the detection of the presence of some additives and preservatives in raw cow milk obtained from rural area of Omdurman city Sudan was done. The milk samples (n= 120) were obtained from rural areas of North and South Omdurman. Significantly, (P≤0.05) higher values for the chemical constituents of the examined raw milk samples were reported in the samples from South Omdurman compared to those from North Omdurman. In addition, the results showed that the added water was found in 16.7% of the examined milk samples. Also, formaldehyde was used as a preservative in 19.2% of the samples, while boric acid was used as preservative in 25% of the milk samples in South Omdurman. However, sodium bicarbonate, hydrogen peroxide and starch were not detected. It was concluded that milk distributed in North and South Omdurman contains some adulterants. Hence control and monitoring programs should be directed towards the hygienic quality of milk during production and marketing.
本研究对产自苏丹奥姆杜尔曼市农村地区的生牛奶进行了化学成分测定和添加剂、防腐剂的检测。牛奶样本(n= 120)来自恩图曼北部和南部农村地区。值得注意的是,与北恩图曼相比,南恩图曼的原料奶样品中化学成分的含量更高(P≤0.05)。此外,检测结果显示,16.7%的牛奶样本中发现了添加的水。此外,19.2%的样品中使用了甲醛作为防腐剂,而在南恩图曼,25%的牛奶样品中使用了硼酸作为防腐剂。但未检出碳酸氢钠、过氧化氢和淀粉。结论是分布在恩图曼北部和南部的牛奶含有一些掺假物质。因此,控制和监测程序应针对牛奶在生产和销售过程中的卫生质量。
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引用次数: 0
Structural and functional effects of single nucleotide polymorphism on canine cytochrome b 5 reductase - in silico 犬细胞色素b5还原酶-硅单核苷酸多态性的结构和功能影响
Pub Date : 2021-09-01 DOI: 10.31559/vmph2021.2.3.2
M. S. Ibrahim
The objectives of this study were to investigate the effects of single nucleotide polymorphism in Canine cytochrome b5 reductase using computational methods. Data was obtained from database of National Centre for Biotechnology Information (db SNP) and computational software was used for the analysis. The 3D protein structure was predicted using phyre 2 server. PANTHER analysis predicted the effect of single nucleotide polymorphism (substitution of Isoleucine for Leucine at position 194) as damaging. Analysis using the Mutpred 2 web application also indicated deleterious effects of the amino acid substitution. Molecular mechanisms of structural changes in the amino acid were determined using Mutpred 2 to be altered ordered interface, gain of allosteric sites and altered metal binding. The study indicated that the substitution of Isoleucine by Leucine at position 194 of the amino acid sequence (Ile 194 Leu) resulted in the destabilization of the amino acid structure leading to functional deviation in canine cytochrome b5 reductase.
本研究的目的是利用计算方法研究单核苷酸多态性对犬细胞色素b5还原酶的影响。数据来源于国家生物技术信息中心数据库(db SNP),采用计算软件进行分析。利用phyre2 server对蛋白的三维结构进行预测。PANTHER分析预测单核苷酸多态性(异亮氨酸取代194位亮氨酸)的影响是有害的。使用Mutpred 2 web应用程序的分析也表明了氨基酸取代的有害影响。利用Mutpred 2确定了氨基酸结构变化的分子机制,以改变有序界面,获得变构位点和改变金属结合。研究表明,犬细胞色素b5还原酶的氨基酸序列(Ile 194 Leu)第194位被亮氨酸取代异亮氨酸导致氨基酸结构不稳定,导致功能偏离。
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引用次数: 1
Prevalence of gastrointestinal nematodes of cattle slaughtered at the lugbe slaughter slab, federal capital territory (FCT), Abuja 在阿布贾联邦首都地区lugbe屠宰场屠宰的牛的胃肠道线虫患病率
Pub Date : 2020-09-01 DOI: 10.31559/vmph2020.1.3.11
Onyekanihu Amaka Lilian, A. O. Bukola, T. Sam, O. N. Maxwell, M. Rabiu
Gastrointestinal nematodes (GIN) are known to be major constraint to cattle well-being and productive performance worldwide. Their prevalence in Abuja Municipal Area Council (AMAC), Abuja is not well studied. This study was carried out to investigate the prevalence of nematodes of cattle slaughtered at the Lugbe slaughter slab of the Federal Capital Territory, Abuja, Nigeria. Between the months of November 2017 and March 2018, a total of two hundred (200) faecal samples were collected and analysed using direct and floatation methods. Of these, 66 (33%) were infected with gastrointestinal nematode parasites. Five (5) genera of nematodes were identified; Ascaris spp, 24 (36.4%); Strongyles spp, 20 (30.3%); Trichostrongylus spp, 10 (15.2%); Nematodirus spp, 8 (12.1%) and Trichuris spp, 4 (6.1%). Mixed infection of these parasites involved oocysts which accounted for 27 (13.5%) and was statistically significant (p<0.05). In relation to sex, prevalence in female (55%) were higher than male (28%), while adults had higher prevalence (36.4%) than in the young (29.9%). The prevalence rate of nematode parasite ova was higher in both female and young cattle than the male and adult cattle, respectively. There was no statistical difference (P>0.05) between the age of cattle and prevalence of nematode parasites. Statistical results showed no association (P>0.05) between the nematode parasite ova and the months which the cattle were slaughtered. However, there was an association (P<0.5) between the prevalence of nematode parasites and the sex of the cattle. Our findings revealed that there were GIN in the screened animals. Appropriate nematode parasites control measures using novel and sustainable approaches including sustainable integrated parasite management (sIPM) have been recommended.
众所周知,胃肠道线虫(GIN)是全球牛健康和生产性能的主要制约因素。它们在阿布贾市地区委员会(AMAC)的流行情况没有得到很好的研究。开展这项研究是为了调查在尼日利亚阿布贾联邦首都地区Lugbe屠宰场屠宰的牛的线虫流行情况。在2017年11月至2018年3月期间,共收集了200份粪便样本,并使用直接法和浮法进行了分析。其中66例(33%)感染了胃肠道线虫寄生虫。鉴定出线虫5属;蛔虫属24只(36.4%);Strongyles spp, 20只(30.3%);毛线虫10株(15.2%);线虫8种(12.1%),滴虫4种(6.1%)。牛卵囊混合感染27例(13.5%),牛年龄与线虫寄生虫感染率之间有统计学意义(p0.05)。统计结果显示,线虫虫卵与屠宰月份之间无显著相关性(P < 0.05)。然而,线虫寄生虫的患病率与牛的性别之间存在相关性(P<0.5)。我们的研究结果表明,筛选的动物中存在GIN。建议采用新颖和可持续的方法,包括可持续寄生虫综合管理(sIPM),采取适当的线虫寄生虫控制措施。
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引用次数: 0
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Veterinary Medicine and Public Health Journal
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