Pub Date : 2022-01-01DOI: 10.1590/2175-7860202273003
L. Matias, Hugo Pereira do Nascimento
Abstract This floristic survey of Alismataceae from the state of Espírito Santo aims to update the diversity and extinction vulnerability data for the species. The taxa here listed were obtained from Splink and Reflora Virtual Herbarium databases. A total of seven native taxa were registered for Espírito Santo state: Echinodorus grandiflorus, E. macrophyllus, Helanthium bolivianus, Hydrocleis nymphoides, Limnocharis flava, Sagittaria lancifolia and S. rhombifolia; the exotic species Alisma plantago-aquatica presented one single record. The populations occurred in flooded areas located at low altitudes and were primarily collected from some preserved areas and in the coastal region of the state. This study presents a taxonomic identification key, morphological descriptions and species illustrations. Comments on ecology, conservation and geographical distribution of the species are also presented.
{"title":"Flora of Espírito Santo, Brazil: Alismataceae","authors":"L. Matias, Hugo Pereira do Nascimento","doi":"10.1590/2175-7860202273003","DOIUrl":"https://doi.org/10.1590/2175-7860202273003","url":null,"abstract":"Abstract This floristic survey of Alismataceae from the state of Espírito Santo aims to update the diversity and extinction vulnerability data for the species. The taxa here listed were obtained from Splink and Reflora Virtual Herbarium databases. A total of seven native taxa were registered for Espírito Santo state: Echinodorus grandiflorus, E. macrophyllus, Helanthium bolivianus, Hydrocleis nymphoides, Limnocharis flava, Sagittaria lancifolia and S. rhombifolia; the exotic species Alisma plantago-aquatica presented one single record. The populations occurred in flooded areas located at low altitudes and were primarily collected from some preserved areas and in the coastal region of the state. This study presents a taxonomic identification key, morphological descriptions and species illustrations. Comments on ecology, conservation and geographical distribution of the species are also presented.","PeriodicalId":38672,"journal":{"name":"Rodriguesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67282751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2175-7860202273020
Claudio Nicoletti de Fraga, E. P. Fernandez, André Paviotti Fontana
Abstract The Flora of Espírito Santo presents three species of Helieae (Gentianaceae), circumscribed in the genera Prepusa, with two species, and Senaea with only one species. These poorly known species are described and illustrated here, and had their diagnostic characters and their similarities with other species discussed. In addition, this paper provides an emended key for species and geographic distribution maps of all species of Prepusa and Senaea from Flora of Espírito Santo, with additional comments on their conservation status.
{"title":"Flora of Espírito Santo: Prepusa and Senaea (Helieae, Gentianaceae)","authors":"Claudio Nicoletti de Fraga, E. P. Fernandez, André Paviotti Fontana","doi":"10.1590/2175-7860202273020","DOIUrl":"https://doi.org/10.1590/2175-7860202273020","url":null,"abstract":"Abstract The Flora of Espírito Santo presents three species of Helieae (Gentianaceae), circumscribed in the genera Prepusa, with two species, and Senaea with only one species. These poorly known species are described and illustrated here, and had their diagnostic characters and their similarities with other species discussed. In addition, this paper provides an emended key for species and geographic distribution maps of all species of Prepusa and Senaea from Flora of Espírito Santo, with additional comments on their conservation status.","PeriodicalId":38672,"journal":{"name":"Rodriguesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67283041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2175-7860202273021
P. Schwartsburd, N. T. L. Pena
Abstract As part of the Flora of Espírito Santo project, we present the taxonomic treatment of the fern family Saccolomataceae, with a key, descriptions, illustrations, and a list of examined specimens. Two species of Saccoloma occur in the state of Espírito Santo: S. elegans and S. nigrescens. Both species are endemic to the Brazilian Atlantic Rainforest.
{"title":"Flora of Espírito Santo: Saccolomataceae","authors":"P. Schwartsburd, N. T. L. Pena","doi":"10.1590/2175-7860202273021","DOIUrl":"https://doi.org/10.1590/2175-7860202273021","url":null,"abstract":"Abstract As part of the Flora of Espírito Santo project, we present the taxonomic treatment of the fern family Saccolomataceae, with a key, descriptions, illustrations, and a list of examined specimens. Two species of Saccoloma occur in the state of Espírito Santo: S. elegans and S. nigrescens. Both species are endemic to the Brazilian Atlantic Rainforest.","PeriodicalId":38672,"journal":{"name":"Rodriguesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67283053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2175-7860202273022
Marco Octávio de Oliveira Pellegrini, Julia Cristina Guarnier
Abstract Pontederiaceae is a small family of Monocots, composed of two genera (i.e., Heteranthera and Pontederia) and ca. 40 species. Two genera and 27 species are accepted for Brazil. In the state of Espírito Santo, Heteranthera is represented by two species and Pontederia by four species. Its species are exclusively found growing in aquatic or paludal, perennial or seasonal environments. We present descriptions, comments, and illustrations for the species.
{"title":"Flora of Espírito Santo: Pontederiaceae","authors":"Marco Octávio de Oliveira Pellegrini, Julia Cristina Guarnier","doi":"10.1590/2175-7860202273022","DOIUrl":"https://doi.org/10.1590/2175-7860202273022","url":null,"abstract":"Abstract Pontederiaceae is a small family of Monocots, composed of two genera (i.e., Heteranthera and Pontederia) and ca. 40 species. Two genera and 27 species are accepted for Brazil. In the state of Espírito Santo, Heteranthera is represented by two species and Pontederia by four species. Its species are exclusively found growing in aquatic or paludal, perennial or seasonal environments. We present descriptions, comments, and illustrations for the species.","PeriodicalId":38672,"journal":{"name":"Rodriguesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67283090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2175-7860202273031
Fátima Conceição de Jesus Freire, J. Silva-Pinheiro, Jayne Silva Santos, Arthur Gomes Lima da Silva, L. S. Camargos, L. Endres, G. C. Justino
Abstract Water deficiency is a major abiotic stress that limits biomass production and drives plant species distributions. We evaluate the effects of water deficiency on ecophysiological and biochemical parameters of seedlings of Tabebuia aurea. Plants were subjected to daily watering (control) and to stress by soil water deficiency for 29 days. Leaf area, plant biomass, gas exchange, SPAD index, maximum quantum yield (Fv / Fm), quantum yield of PSII (ΦPSII), superoxide dismutase (SOD) and L-ascorbate peroxidase (APX) activity, lipid peroxidation, and proline content were recorded. Plants responded to water deficit by reducing leaf area and accumulating proline. Stomatal conductance was reduced to limit the water loss by transpiration. However, limiting CO2 uptake caused reduction in photosynthesis and biomass. The excess of energy unutilized by photosynthesis reduced SPAD index and ΦPSII. As a result, we observed an increase in SOD and APX activity, protecting chloroplast membranes from further damages caused by lipid peroxidation. Our results indicate that T. aurea have capacity to survive under water deficiency reducing stomatal aperture, but affecting the rate of CO2 assimilation. Nevertheless, plants showed mechanisms to preventing damages to the photosynthetic apparatus. Such plasticity is an important adaptation for plants growing in dry environmental.
{"title":"Proline and antioxidant enzymes protect Tabebuia aurea (Bignoniaceae) from transitory water deficiency","authors":"Fátima Conceição de Jesus Freire, J. Silva-Pinheiro, Jayne Silva Santos, Arthur Gomes Lima da Silva, L. S. Camargos, L. Endres, G. C. Justino","doi":"10.1590/2175-7860202273031","DOIUrl":"https://doi.org/10.1590/2175-7860202273031","url":null,"abstract":"Abstract Water deficiency is a major abiotic stress that limits biomass production and drives plant species distributions. We evaluate the effects of water deficiency on ecophysiological and biochemical parameters of seedlings of Tabebuia aurea. Plants were subjected to daily watering (control) and to stress by soil water deficiency for 29 days. Leaf area, plant biomass, gas exchange, SPAD index, maximum quantum yield (Fv / Fm), quantum yield of PSII (ΦPSII), superoxide dismutase (SOD) and L-ascorbate peroxidase (APX) activity, lipid peroxidation, and proline content were recorded. Plants responded to water deficit by reducing leaf area and accumulating proline. Stomatal conductance was reduced to limit the water loss by transpiration. However, limiting CO2 uptake caused reduction in photosynthesis and biomass. The excess of energy unutilized by photosynthesis reduced SPAD index and ΦPSII. As a result, we observed an increase in SOD and APX activity, protecting chloroplast membranes from further damages caused by lipid peroxidation. Our results indicate that T. aurea have capacity to survive under water deficiency reducing stomatal aperture, but affecting the rate of CO2 assimilation. Nevertheless, plants showed mechanisms to preventing damages to the photosynthetic apparatus. Such plasticity is an important adaptation for plants growing in dry environmental.","PeriodicalId":38672,"journal":{"name":"Rodriguesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67283124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2175-7860202273024
Fernanda Savioli Scaravelli, P. H. Gaem, K. S. Valdemarin, E. Lucas, F. Mazine
Abstract Myrcia (Myrteae, Myrtaceae) is represented by 101 species in the Brazilian state of Espírito Santo, but local floristic surveys on this genus still lack in this centre of diversity and endemism. In this context, a taxonomic account of Myrcia in the Vale Natural Reserve (VNR), Linhares, Espírito Santo, is presented. Forty-six species were registered in this study, representing 46% of the richness of the genus in Espírito Santo. Twenty-five species (54%) are endemic to the Atlantic domain and seven are endemic to Espírito Santo. Myrcia scytophylla is reported in the Atlantic forest for the first time and M. cymatophylla, M. congestiflora, and M. ferruginosa are new occurrences in the state. Species composition revealed by this study considerably differs from that of a previous checklist. Representatives of Myrcia can be found in all main vegetation types of the VNR and the anthropic areas. The study site must be considered prioritary for conservation of Myrcia due to the presence of a rich assemblage, which also includes restrict and threatened species.
{"title":"Myrcia (Myrtaceae) in the Vale Natural Reserve, Linhares, Espírito Santo, Brazil","authors":"Fernanda Savioli Scaravelli, P. H. Gaem, K. S. Valdemarin, E. Lucas, F. Mazine","doi":"10.1590/2175-7860202273024","DOIUrl":"https://doi.org/10.1590/2175-7860202273024","url":null,"abstract":"Abstract Myrcia (Myrteae, Myrtaceae) is represented by 101 species in the Brazilian state of Espírito Santo, but local floristic surveys on this genus still lack in this centre of diversity and endemism. In this context, a taxonomic account of Myrcia in the Vale Natural Reserve (VNR), Linhares, Espírito Santo, is presented. Forty-six species were registered in this study, representing 46% of the richness of the genus in Espírito Santo. Twenty-five species (54%) are endemic to the Atlantic domain and seven are endemic to Espírito Santo. Myrcia scytophylla is reported in the Atlantic forest for the first time and M. cymatophylla, M. congestiflora, and M. ferruginosa are new occurrences in the state. Species composition revealed by this study considerably differs from that of a previous checklist. Representatives of Myrcia can be found in all main vegetation types of the VNR and the anthropic areas. The study site must be considered prioritary for conservation of Myrcia due to the presence of a rich assemblage, which also includes restrict and threatened species.","PeriodicalId":38672,"journal":{"name":"Rodriguesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67283142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2175-7860202273065
A. B. Oliveira, G. Antar, M. Trovó
Abstract This study details the floristic diversity of Mentheae (Lamiaceae - Nepetoideae) from the Itatiaia National Park, the first Protected Area in Brazil. Among the 30 species of Lamiaceae cited for the Park, we recorded 15 species belonging to Mentheae arranged in seven genera: Cunila (2 spp.), Hedeoma (1 sp.), Hesperozygis (1 sp.), Lepechinia (1 sp.), Prunella (1 sp.), Rhabdocaulon (1 sp.), and Salvia (8 spp.). The region presents significant diversity, considering the 62 native species of Mentheae recorded in the Brazilian Southeast. The species are distributed in the Park’s different vegetation types, mostly in shaded areas of the ombrophilous forests or throughout high-altitude grasslands. We provide a complete description for each species and an identification key, as well as comments on species distribution, habitat, morphological variation, and phenology.
{"title":"Mentheae (Nepetoideae - Lamiaceae) from the Itatiaia National Park, Brazil","authors":"A. B. Oliveira, G. Antar, M. Trovó","doi":"10.1590/2175-7860202273065","DOIUrl":"https://doi.org/10.1590/2175-7860202273065","url":null,"abstract":"Abstract This study details the floristic diversity of Mentheae (Lamiaceae - Nepetoideae) from the Itatiaia National Park, the first Protected Area in Brazil. Among the 30 species of Lamiaceae cited for the Park, we recorded 15 species belonging to Mentheae arranged in seven genera: Cunila (2 spp.), Hedeoma (1 sp.), Hesperozygis (1 sp.), Lepechinia (1 sp.), Prunella (1 sp.), Rhabdocaulon (1 sp.), and Salvia (8 spp.). The region presents significant diversity, considering the 62 native species of Mentheae recorded in the Brazilian Southeast. The species are distributed in the Park’s different vegetation types, mostly in shaded areas of the ombrophilous forests or throughout high-altitude grasslands. We provide a complete description for each species and an identification key, as well as comments on species distribution, habitat, morphological variation, and phenology.","PeriodicalId":38672,"journal":{"name":"Rodriguesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67283600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2175-7860202273050
A. M. Martelo-Solorzano, K. I. Lidueña-Pérez, J. P. Corredor-Prado
Abstract Drastic changes in dry tropical forest result in the loss of biological components and reveal the importance of studies on the biology of species living in it. The present study aimed to describe seed morphoanatomy, germination and post-seminal development of Bromeliaceae species in fragments of tropical dry forest in Sucre, Colombia. Seven species representing Bromelia and Tillandsia genera were evaluated. The results provide characteristics related to the habitat of each species and contribute to distinguish the genera evaluated: fruit and seed measurements, seed shape, plumose appendage, testa characteristics, and the aleurone layer, embryo endosperm ratio, types of reserves, constriction zone in the embryo and type of post-seminal development. Characteristics of plumose appendages and the presence of vascular bundles in the embryo also contribute to distinguish Tillandsia species. In T. elongata and T. flexuosa, high number of seeds per fruit (> 100), morphoanatomical aspects, high germination (> 92%) and plant formation (> 77%) percentages, and higher germination rate values (> 4.5) give them the potential capacity for establishment in this environment. Our results provide information with taxonomic and ecological relevance for bromeliads in dry tropical forest.
{"title":"Seed’s morpho-anatomy and post-seminal development of Bromeliaceae from tropical dry forest","authors":"A. M. Martelo-Solorzano, K. I. Lidueña-Pérez, J. P. Corredor-Prado","doi":"10.1590/2175-7860202273050","DOIUrl":"https://doi.org/10.1590/2175-7860202273050","url":null,"abstract":"Abstract Drastic changes in dry tropical forest result in the loss of biological components and reveal the importance of studies on the biology of species living in it. The present study aimed to describe seed morphoanatomy, germination and post-seminal development of Bromeliaceae species in fragments of tropical dry forest in Sucre, Colombia. Seven species representing Bromelia and Tillandsia genera were evaluated. The results provide characteristics related to the habitat of each species and contribute to distinguish the genera evaluated: fruit and seed measurements, seed shape, plumose appendage, testa characteristics, and the aleurone layer, embryo endosperm ratio, types of reserves, constriction zone in the embryo and type of post-seminal development. Characteristics of plumose appendages and the presence of vascular bundles in the embryo also contribute to distinguish Tillandsia species. In T. elongata and T. flexuosa, high number of seeds per fruit (> 100), morphoanatomical aspects, high germination (> 92%) and plant formation (> 77%) percentages, and higher germination rate values (> 4.5) give them the potential capacity for establishment in this environment. Our results provide information with taxonomic and ecological relevance for bromeliads in dry tropical forest.","PeriodicalId":38672,"journal":{"name":"Rodriguesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67283614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2175-7860202273041
P. Sena, N. Fonsêca, A. C. Lins-e-Silva
Abstract Dead organic matter represents an essential reservoir of carbon, especially that allocated in standing dead trees, coarse woody debris, and fine litter, playing a pivotal role in nutrient cycling and habitat provisioning. However, necromass is frequently disregarded in forest assessments. Here, we aimed to perform the first assessment of multiple necromass compartments in the Atlantic Forest of Northeast Brazil, providing a basis for future integrative studies related to necromass in this region. We registered 17 standing dead trees in 0.5 hectare and 239 logs of coarse woody debris. Necromass had 3.9 Mg.ha-1 of standing dead trees, 54.24 Mg.ha-1 of coarse woody debris and 7.2 Mg.ha-1 of litter. We indicate that standing dead trees and coarse debris were mostly in the intermediate and final stages of decomposition. Leaves were the dominant component of litter, and drier months had more litterfall. Finally, we highlight that assessing standing dead trees and coarse woody debris adds 25.6% on top of aboveground tree mass, improving information about organic matter storage in rainforest ecosystems. Our findings emphasize that the necromass compartment must be considered in forest assessments, also including small pieces of coarse woody debris, which could inform better practices of forest management.
{"title":"Non-negligible role of dead organic matter in a rainforest remnant in Northeast Brazil","authors":"P. Sena, N. Fonsêca, A. C. Lins-e-Silva","doi":"10.1590/2175-7860202273041","DOIUrl":"https://doi.org/10.1590/2175-7860202273041","url":null,"abstract":"Abstract Dead organic matter represents an essential reservoir of carbon, especially that allocated in standing dead trees, coarse woody debris, and fine litter, playing a pivotal role in nutrient cycling and habitat provisioning. However, necromass is frequently disregarded in forest assessments. Here, we aimed to perform the first assessment of multiple necromass compartments in the Atlantic Forest of Northeast Brazil, providing a basis for future integrative studies related to necromass in this region. We registered 17 standing dead trees in 0.5 hectare and 239 logs of coarse woody debris. Necromass had 3.9 Mg.ha-1 of standing dead trees, 54.24 Mg.ha-1 of coarse woody debris and 7.2 Mg.ha-1 of litter. We indicate that standing dead trees and coarse debris were mostly in the intermediate and final stages of decomposition. Leaves were the dominant component of litter, and drier months had more litterfall. Finally, we highlight that assessing standing dead trees and coarse woody debris adds 25.6% on top of aboveground tree mass, improving information about organic matter storage in rainforest ecosystems. Our findings emphasize that the necromass compartment must be considered in forest assessments, also including small pieces of coarse woody debris, which could inform better practices of forest management.","PeriodicalId":38672,"journal":{"name":"Rodriguesia","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67283817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1590/2175-7860202273043
W. D. M. Ferreira, Adriana Magno de Oliveira, Joyce Cavalcante Viana, R. M. Suzuki, J. R. G. D. Oliveira
Abstract Cyrtopodium paludicolum is a terrestrial orchid species, native to Brazil, whose natural propagation is jeopardized by the intensive collection from the wild and is threatened by agricultural expansion in Cerrado areas. In light of that, this investigation aimed at studying the in vitro germination and early development of C. paludicolum as influenced by culture medium, sucrose and growth regulators as well as its micropropagation by using dark-grown stem segments. A protocol for its acclimatization is also detailed. The effects of Murashige & Skoog (MS), Knudson C (KC) and Vacin & Went (VW) media on the in vitro germination and initial development were tested. The influence of different concentrations of BA, NAA, and of sucrose on plant multiplication and growth were evaluated. The possibility of using etiolated stem segments for micropropagation was also assessed. Acclimatization was accomplished in two phases by using three different substrates. The results showed that VW was the best medium for germination whereas for seedling formation KC was the most advantageous since they were healthy and vigorous. Sucrose at 2% favored the greatest seedling growth and development. Shoot and root proliferation and development were best promoted in the presence of 2.28/2.28 and 0.57/0.57 µM BA/NAA, respectively. The use of etiolated stem segments for micropropagation was effective. Successful acclimatization was accomplished by initially growing plants in community pots containing a 3:1 (v/v) mix of Bioplant and dried Sphagnum moss followed by their transfer to individual pots containing a 2:1 (v/v) mix of Bioplant and Ouro Negro substrates.
摘要:Cyrtopodium paludicolum是一种原产于巴西的陆生兰花，其自然繁殖受到野生采收和塞拉多地区农业扩张的威胁。鉴于此，本研究旨在研究培养基、蔗糖和生长调节剂对苜蓿离体萌发和早期发育的影响，并利用暗生茎段进行微繁。本文还详细介绍了其驯化方案。研究了Murashige & Skoog (MS)、Knudson C (KC)和Vacin & go (VW)培养基对离体萌发和初期发育的影响。研究了不同浓度的BA、NAA和蔗糖对植株增殖和生长的影响。利用黄化茎段进行微繁的可能性也进行了评估。采用三种不同的基质，分两个阶段进行驯化。结果表明，以VW培养基萌发效果最好，以KC培养基成苗效果最好，因其健康健壮。蔗糖浓度为2%时对幼苗生长发育最有利。BA/NAA浓度分别为2.28/2.28µM和0.57/0.57µM时，茎和根的增殖发育效果最好。利用黄化茎段进行微繁是有效的。成功的驯化是通过最初在含有3:1 (v/v)混合的生物植物和干苔藓的群落盆栽中种植植物，然后将其转移到含有2:1 (v/v)混合的生物植物和欧鲁黑底物的个体盆栽中完成的。
{"title":"Asymbiotic germination, initial development in vitro and acclimatization of Cyrtopodium paludicolum Hoehne, a Brazilian Savanna orchid species","authors":"W. D. M. Ferreira, Adriana Magno de Oliveira, Joyce Cavalcante Viana, R. M. Suzuki, J. R. G. D. Oliveira","doi":"10.1590/2175-7860202273043","DOIUrl":"https://doi.org/10.1590/2175-7860202273043","url":null,"abstract":"Abstract Cyrtopodium paludicolum is a terrestrial orchid species, native to Brazil, whose natural propagation is jeopardized by the intensive collection from the wild and is threatened by agricultural expansion in Cerrado areas. In light of that, this investigation aimed at studying the in vitro germination and early development of C. paludicolum as influenced by culture medium, sucrose and growth regulators as well as its micropropagation by using dark-grown stem segments. A protocol for its acclimatization is also detailed. The effects of Murashige & Skoog (MS), Knudson C (KC) and Vacin & Went (VW) media on the in vitro germination and initial development were tested. The influence of different concentrations of BA, NAA, and of sucrose on plant multiplication and growth were evaluated. The possibility of using etiolated stem segments for micropropagation was also assessed. Acclimatization was accomplished in two phases by using three different substrates. The results showed that VW was the best medium for germination whereas for seedling formation KC was the most advantageous since they were healthy and vigorous. Sucrose at 2% favored the greatest seedling growth and development. Shoot and root proliferation and development were best promoted in the presence of 2.28/2.28 and 0.57/0.57 µM BA/NAA, respectively. The use of etiolated stem segments for micropropagation was effective. Successful acclimatization was accomplished by initially growing plants in community pots containing a 3:1 (v/v) mix of Bioplant and dried Sphagnum moss followed by their transfer to individual pots containing a 2:1 (v/v) mix of Bioplant and Ouro Negro substrates.","PeriodicalId":38672,"journal":{"name":"Rodriguesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67283888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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